LOC108628482 cDNA ORF clone, Ceratina calcarata

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC108628482
Entrez Gene ID	108628482
Full Name	anoctamin-8-like
Gene Type	protein-coding
Organism	Ceratina calcarata

mRNA and Protein(s)

mRNA	Protein	Name
XM_018030436.1	XP_017885925.1	anoctamin-8-like isoform X2
XM_018030438.2	XP_017885927.1	anoctamin-8-like isoform X4
XM_018030435.1	XP_017885924.1	anoctamin-8-like isoform X1
XM_018030437.1	XP_017885926.1	anoctamin-8-like isoform X3
XM_018030436.2	XP_017885925.1	anoctamin-8-like isoform X2
XM_018030438.1	XP_017885927.1	anoctamin-8-like isoform X4
XM_018030435.2	XP_017885924.1	anoctamin-8-like isoform X1
XM_018030437.2	XP_017885926.1	anoctamin-8-like isoform X3

Pathways from BioSystems
Gene Ontology

The following LOC108628482 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC108628482 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe149339	XM_018030435.2 Latest version!	Ceratina calcarata anoctamin-8-like (LOC108628482), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OCe149340	XM_018030436.2 Latest version!	Ceratina calcarata anoctamin-8-like (LOC108628482), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OCe149341	XM_018030437.2 Latest version!	Ceratina calcarata anoctamin-8-like (LOC108628482), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OCe149342	XM_018030438.2 Latest version!	Ceratina calcarata anoctamin-8-like (LOC108628482), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
View Old Accession Versions >>
OCe149340	XM_018030436.1	Ceratina calcarata anoctamin-8-like (LOC108628482), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OCe149342	XM_018030438.1	Ceratina calcarata anoctamin-8-like (LOC108628482), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OCe149339	XM_018030435.1	Ceratina calcarata anoctamin-8-like (LOC108628482), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OCe149341	XM_018030437.1	Ceratina calcarata anoctamin-8-like (LOC108628482), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC108628482 ( XM_018030435.1 ) cDNA ORF clone, Ceratina calcarata -> XP_017885924.1 Ceratina calcarata anoctamin-8-like (LOC108628482), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe149339
Clone ID Related Accession (Same CDS sequence)	XM_018030435.1 , XM_018030435.2
Accession Version	XM_018030435.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3396bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-08-30
Organism	Ceratina calcarata
Product	anoctamin-8-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017176396.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ceratina calcarata Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAGGCG GCGAGGGGTC CCCGATCAAT CTCCTCGACC AGCATCGTGC ATCGACCACC 
TCCGGCGAGT GCGCGAGTAC AAGTGACGAT GATGAGAAGA CTGCCACGGG CACCGTTTCC 
AGCACGATGA CGACCGTCTT CGCGGGCGAC GGTCTCAGAC GGAGGAAGGT CATACACGCG 
GCCAAAGAAA CCCTCGACAA GGCTTCGCGT CTGCTCAGAC GGAAGATACC ATGCACAGGC 
CACTTGATGA CCCCCCGCCG CCTATGGTTA CAAAAGGTTC CAACGCAGGA ATGCGACGTC 
GTGATGATGT TCCCGAGCGG AGCGAGCGAC GAGACCCTGA TGTGGCTTCT AGGCCGACTT 
CGGGCTGGAA CCCCGGGCTT GGTGGTGCAC GTGCGACACC ATGCCTCTTC AGATAGTTAC 
AGCTTTTACT TAACTGCGCC GTTTAGCGTA CTTTTGAAAG CAGCTGAGGA GGTGCATTTA 
CCGAAGACGC TGCGACAGGA GTTCGGCGGT GGACTGAAGG AGTTCGTTGG CTCCGAGGCG 
AGCTGCTTCG AGGGTAGTGA CGATGAAGCT CAGTTCTTCA CCACCCAGGA GAGGCAATCG 
CTGGTACTCC ACCTGCTGCA CACTCTCAGG GCGGGCCCAC AGGACCTCCA CAGCCTGGCC 
GGGCTCAAGA TGGTCGAAGG TCAAGCAATT ATTCCAAAGT GCATTTCAGC TGGGATCATA 
TCCCAGGTCT TCCCTCTTCA CGAATTGCCT GCGTTGGAGA AACTGCAACG GACATGGGTT 
CGTGCGTTTC TCAGCCCCCA ACCCCTGGAC GACATTTGTA AATACTTCGG AGTCAAGATT 
ACCATGTATT TCGCGTGGCT CGGTCATTAC ACCACCGCTT TGATAGTTCC AGCTGTAGTG 
GGTGCCATAT ATTGGATCGG CATCATCGGT AGGAATCAAG CAGTGGAGGA CGTGGCGTAC 
GTACTGTTCT CTGTGTTCAA CGTAATCTGG GCCACCGTTT ACCTGGAAAC TTGGAAAAGA 
AGGGGTGCCG AACTGGCCTA CAGGTGGGGC ACGTTGGATC AAAGGGACGA CCTACTTCTC 
GAACCTAGGC CCTTATTCAC GGGTACCTTG GAAATTTCAT CGGTGACTGG AAGACTGGAG 
CCGACGTACC CTAGATGGAA GAGGAACATG TTCCGGTATT TCGTCAGCGT GCCCATCATT 
GCAGCGTGCC TGTTCTTCGT TTTCATCGTG ATGATTCTCA GCTTTCAGAT ACAGGATTGG 
TGGGACGCAC GCCTCGAGTC TAGGGGATAC GGTTTCTGGT TGAGCTACGT GCCAAAAGTC 
TTACTCGCGG TGGTGATAGC TTTAATGGAC GAGGCTTACT TCAAGGTTGC CGTATGGTTG 
AACGACATGG AAAACTATCG GCTAGACACC GAGTACGAGA ATCATCTGAT CTACAAGGTG 
GCACTGTTTC AGTTTGTAAA CTCCTTCCTA TCGCTATTTT ACATCGCCTT CTACCTACAG 
GATCAGGAGA GGCTGAAAGA GCAATTGGCC GCTCTGCTGA TAGTCCGGCA GGTGATCGGG 
CATCTGAAAG AGTCTGCAGT GCCGTATCTG ATCGAACAGC TACGACTCGC AAGGTTGAGC 
TTCGAGCTTT TCGGCGCGCT GAGCCCCAGT GAGGCTCGTC CTCCTCCTGG CGAGGACACC 
GAGGAAAATG GGAAGCAGGA GGGCGGCGAT GAGAAGAACG ATCGACCGGA CAGTGCGAAA 
GCGAAGCAGA GAAACGTCAG CCAGGCCGAG CTGGAGAGTT CCCTCTACAG AGTAGGGCAT 
CCCACTAATT CTCTACTTAG TGACGTTACG TTCAAGTACG ACGGGGCGTT TTCAGAGCAT 
CTGGAAATGC TGTCGCAACT GGGATACGTC TGCCTGTTCT CCTCGGCTTT CCCTCTGGCA 
GCGATGTTAG CGTTGCTTGG AAATCTTTTT GAGCTACGAG GCGACGCTTT CAAGCTGTGC 
TTTGTACTTC AGCGACCGTT CGGCCGTCGG GTCTCCAGCA TTGGAACTTG GCAGAACGCC 
ATGGAGGCGA TGGGTCTGGT CGCTATTTTG GTGAACTGCG CGCTGATCGG TCTCAGCGGG 
CAGGTTCAAC GGATGTTCCC GGAGATGTCG GCCACTCAGA CGATTCTGTT GATAGTTGCC 
CTTGAACATA TCATGCTGGC GATACGATTC ATCATAATCT GCGCGATTCC CGACATACCG 
CATTGGGTGG CCACGGAAAT GGCCAAGGTA GAGTTCCTGA GGAGAGAGGC GGTCAGAAGG 
TTATCCTCAA CGCCATCACC GGAGCAGCAA CCGGCTACAG TGATAGGGAG ATTCGTGGTG 
AGTCCAGCAG ACGGTGAGAG CGAAGAGCAA CAATTGTTGT CAGAGGGCAA CGGCGACGCG 
ACGCCCTCCA GCATCCCGGA CACGCCGACG CTGGCGTCGA CCACCCAGAC GCCGAGCAGC 
CCTCCAACTC CTAGTGGTGC ATCCGTGCCG AGGATCTCGG AGACACCCTC CGCGGCTCAA 
ACTCCGGTCA GCTGTGCCAG CAGCGACTTG GGTAGTCCTT TACTGACCGA CAAGAGCATC 
ATAGGCAGCG TCCGAGACAT TCACCACGCG CCTAGGTGTT CCATTCCCGG TGGAGAACGA 
GGAAGGCGTT CAAGGGACTG GCTACAGACT GAATCGGAAG CGTCGGGTGG TGGCGATCAG 
TACAGCCACC ATCTGACGAT CGGTCCTCAC GGTGGTATAG ACTGGGTCCG AAGATTAGGT 
CTCGAGACTG CTGTTCGAAA ATCCAGCGAC TCCGAGATCA GCGGAGCTGG TACCGTCAGT 
GGAAGTGGAG ACGAGTTGAC TCTTCACAGG TCTACTGACT GCATCGTCTC GAAAGAACTC 
GCATCCTCAT CGGATAGCGA TCTCCTCAGA TCCGCCCCGC CATGGTCAGT GGCTCACAGG 
AATCAGAAGT TCCGTTTCTC GCCCGAACGA GAGAAGGAAC GGGAGAAGAC CGAGAAGCAG 
CAGTATCAAC AGTATCAGCG TGAAATTCAA GATCACAAGC AACAAGCAGC GTTGTACGCT 
GAAAAAGAGA AGGAGAAAGA GTCAAGTGCC CTCTCAGACT CGAGCAAAAC TTCCAGTCAG 
GAGGAGGAGA AGCCGTCGAA GGAGGATCGA GAGGCGAAGA AGACCAGGGT GAAGCAGAGT 
TTAATGAAAA GAGCCAGGTC CGTGGCGATC TTCTCGTTAA AGCTGAAGGA GCGGCGCGCG 
AGGGAGGCCG AGATGAAGGC GAAGGAGGCG GAGAGGGAAG CCAGGTGGCA ACAGCCGCAG 
TCCTGCGTCG GCGGCGAACT CTCTTGCATT CCCATAGAAA AGCTTATATC TGTGGACGAC 
ATCGCAGCCA TGGAGTTACG CAGACTTAAT CATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017885924.1
CDS	64..3459
Translation	MPGGEGSPINLLDQHRASTTSGECASTSDDDEKTATGTVSSTMTTVFAGDGLRRRKVIHAAKETLDKASRLLRRKIPCTGHLMTPRRLWLQKVPTQECDVVMMFPSGASDETLMWLLGRLRAGTPGLVVHVRHHASSDSYSFYLTAPFSVLLKAAEEVHLPKTLRQEFGGGLKEFVGSEASCFEGSDDEAQFFTTQERQSLVLHLLHTLRAGPQDLHSLAGLKMVEGQAIIPKCISAGIISQVFPLHELPALEKLQRTWVRAFLSPQPLDDICKYFGVKITMYFAWLGHYTTALIVPAVVGAIYWIGIIGRNQAVEDVAYVLFSVFNVIWATVYLETWKRRGAELAYRWGTLDQRDDLLLEPRPLFTGTLEISSVTGRLEPTYPRWKRNMFRYFVSVPIIAACLFFVFIVMILSFQIQDWWDARLESRGYGFWLSYVPKVLLAVVIALMDEAYFKVAVWLNDMENYRLDTEYENHLIYKVALFQFVNSFLSLFYIAFYLQDQERLKEQLAALLIVRQVIGHLKESAVPYLIEQLRLARLSFELFGALSPSEARPPPGEDTEENGKQEGGDEKNDRPDSAKAKQRNVSQAELESSLYRVGHPTNSLLSDVTFKYDGAFSEHLEMLSQLGYVCLFSSAFPLAAMLALLGNLFELRGDAFKLCFVLQRPFGRRVSSIGTWQNAMEAMGLVAILVNCALIGLSGQVQRMFPEMSATQTILLIVALEHIMLAIRFIIICAIPDIPHWVATEMAKVEFLRREAVRRLSSTPSPEQQPATVIGRFVVSPADGESEEQQLLSEGNGDATPSSIPDTPTLASTTQTPSSPPTPSGASVPRISETPSAAQTPVSCASSDLGSPLLTDKSIIGSVRDIHHAPRCSIPGGERGRRSRDWLQTESEASGGGDQYSHHLTIGPHGGIDWVRRLGLETAVRKSSDSEISGAGTVSGSGDELTLHRSTDCIVSKELASSSDSDLLRSAPPWSVAHRNQKFRFSPEREKEREKTEKQQYQQYQREIQDHKQQAALYAEKEKEKESSALSDSSKTSSQEEEKPSKEDREAKKTRVKQSLMKRARSVAIFSLKLKERRAREAEMKAKEAEREARWQQPQSCVGGELSCIPIEKLISVDDIAAMELRRLNH

Target ORF information:

RefSeq Version	XM_018030435.1
Organism	Ceratina calcarata
Definition	Ceratina calcarata anoctamin-8-like (LOC108628482), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018030435.1

ORF Insert Sequence:

ATGCCAGGCG GCGAGGGGTC CCCGATCAAT CTCCTCGACC AGCATCGTGC ATCGACCACC 
TCCGGCGAGT GCGCGAGTAC AAGTGACGAT GATGAGAAGA CTGCCACGGG CACCGTTTCC 
AGCACGATGA CGACCGTCTT CGCGGGCGAC GGTCTCAGAC GGAGGAAGGT CATACACGCG 
GCCAAAGAAA CCCTCGACAA GGCTTCGCGT CTGCTCAGAC GGAAGATACC ATGCACAGGC 
CACTTGATGA CCCCCCGCCG CCTATGGTTA CAAAAGGTTC CAACGCAGGA ATGCGACGTC 
GTGATGATGT TCCCGAGCGG AGCGAGCGAC GAGACCCTGA TGTGGCTTCT AGGCCGACTT 
CGGGCTGGAA CCCCGGGCTT GGTGGTGCAC GTGCGACACC ATGCCTCTTC AGATAGTTAC 
AGCTTTTACT TAACTGCGCC GTTTAGCGTA CTTTTGAAAG CAGCTGAGGA GGTGCATTTA 
CCGAAGACGC TGCGACAGGA GTTCGGCGGT GGACTGAAGG AGTTCGTTGG CTCCGAGGCG 
AGCTGCTTCG AGGGTAGTGA CGATGAAGCT CAGTTCTTCA CCACCCAGGA GAGGCAATCG 
CTGGTACTCC ACCTGCTGCA CACTCTCAGG GCGGGCCCAC AGGACCTCCA CAGCCTGGCC 
GGGCTCAAGA TGGTCGAAGG TCAAGCAATT ATTCCAAAGT GCATTTCAGC TGGGATCATA 
TCCCAGGTCT TCCCTCTTCA CGAATTGCCT GCGTTGGAGA AACTGCAACG GACATGGGTT 
CGTGCGTTTC TCAGCCCCCA ACCCCTGGAC GACATTTGTA AATACTTCGG AGTCAAGATT 
ACCATGTATT TCGCGTGGCT CGGTCATTAC ACCACCGCTT TGATAGTTCC AGCTGTAGTG 
GGTGCCATAT ATTGGATCGG CATCATCGGT AGGAATCAAG CAGTGGAGGA CGTGGCGTAC 
GTACTGTTCT CTGTGTTCAA CGTAATCTGG GCCACCGTTT ACCTGGAAAC TTGGAAAAGA 
AGGGGTGCCG AACTGGCCTA CAGGTGGGGC ACGTTGGATC AAAGGGACGA CCTACTTCTC 
GAACCTAGGC CCTTATTCAC GGGTACCTTG GAAATTTCAT CGGTGACTGG AAGACTGGAG 
CCGACGTACC CTAGATGGAA GAGGAACATG TTCCGGTATT TCGTCAGCGT GCCCATCATT 
GCAGCGTGCC TGTTCTTCGT TTTCATCGTG ATGATTCTCA GCTTTCAGAT ACAGGATTGG 
TGGGACGCAC GCCTCGAGTC TAGGGGATAC GGTTTCTGGT TGAGCTACGT GCCAAAAGTC 
TTACTCGCGG TGGTGATAGC TTTAATGGAC GAGGCTTACT TCAAGGTTGC CGTATGGTTG 
AACGACATGG AAAACTATCG GCTAGACACC GAGTACGAGA ATCATCTGAT CTACAAGGTG 
GCACTGTTTC AGTTTGTAAA CTCCTTCCTA TCGCTATTTT ACATCGCCTT CTACCTACAG 
GATCAGGAGA GGCTGAAAGA GCAATTGGCC GCTCTGCTGA TAGTCCGGCA GGTGATCGGG 
CATCTGAAAG AGTCTGCAGT GCCGTATCTG ATCGAACAGC TACGACTCGC AAGGTTGAGC 
TTCGAGCTTT TCGGCGCGCT GAGCCCCAGT GAGGCTCGTC CTCCTCCTGG CGAGGACACC 
GAGGAAAATG GGAAGCAGGA GGGCGGCGAT GAGAAGAACG ATCGACCGGA CAGTGCGAAA 
GCGAAGCAGA GAAACGTCAG CCAGGCCGAG CTGGAGAGTT CCCTCTACAG AGTAGGGCAT 
CCCACTAATT CTCTACTTAG TGACGTTACG TTCAAGTACG ACGGGGCGTT TTCAGAGCAT 
CTGGAAATGC TGTCGCAACT GGGATACGTC TGCCTGTTCT CCTCGGCTTT CCCTCTGGCA 
GCGATGTTAG CGTTGCTTGG AAATCTTTTT GAGCTACGAG GCGACGCTTT CAAGCTGTGC 
TTTGTACTTC AGCGACCGTT CGGCCGTCGG GTCTCCAGCA TTGGAACTTG GCAGAACGCC 
ATGGAGGCGA TGGGTCTGGT CGCTATTTTG GTGAACTGCG CGCTGATCGG TCTCAGCGGG 
CAGGTTCAAC GGATGTTCCC GGAGATGTCG GCCACTCAGA CGATTCTGTT GATAGTTGCC 
CTTGAACATA TCATGCTGGC GATACGATTC ATCATAATCT GCGCGATTCC CGACATACCG 
CATTGGGTGG CCACGGAAAT GGCCAAGGTA GAGTTCCTGA GGAGAGAGGC GGTCAGAAGG 
TTATCCTCAA CGCCATCACC GGAGCAGCAA CCGGCTACAG TGATAGGGAG ATTCGTGGTG 
AGTCCAGCAG ACGGTGAGAG CGAAGAGCAA CAATTGTTGT CAGAGGGCAA CGGCGACGCG 
ACGCCCTCCA GCATCCCGGA CACGCCGACG CTGGCGTCGA CCACCCAGAC GCCGAGCAGC 
CCTCCAACTC CTAGTGGTGC ATCCGTGCCG AGGATCTCGG AGACACCCTC CGCGGCTCAA 
ACTCCGGTCA GCTGTGCCAG CAGCGACTTG GGTAGTCCTT TACTGACCGA CAAGAGCATC 
ATAGGCAGCG TCCGAGACAT TCACCACGCG CCTAGGTGTT CCATTCCCGG TGGAGAACGA 
GGAAGGCGTT CAAGGGACTG GCTACAGACT GAATCGGAAG CGTCGGGTGG TGGCGATCAG 
TACAGCCACC ATCTGACGAT CGGTCCTCAC GGTGGTATAG ACTGGGTCCG AAGATTAGGT 
CTCGAGACTG CTGTTCGAAA ATCCAGCGAC TCCGAGATCA GCGGAGCTGG TACCGTCAGT 
GGAAGTGGAG ACGAGTTGAC TCTTCACAGG TCTACTGACT GCATCGTCTC GAAAGAACTC 
GCATCCTCAT CGGATAGCGA TCTCCTCAGA TCCGCCCCGC CATGGTCAGT GGCTCACAGG 
AATCAGAAGT TCCGTTTCTC GCCCGAACGA GAGAAGGAAC GGGAGAAGAC CGAGAAGCAG 
CAGTATCAAC AGTATCAGCG TGAAATTCAA GATCACAAGC AACAAGCAGC GTTGTACGCT 
GAAAAAGAGA AGGAGAAAGA GTCAAGTGCC CTCTCAGACT CGAGCAAAAC TTCCAGTCAG 
GAGGAGGAGA AGCCGTCGAA GGAGGATCGA GAGGCGAAGA AGACCAGGGT GAAGCAGAGT 
TTAATGAAAA GAGCCAGGTC CGTGGCGATC TTCTCGTTAA AGCTGAAGGA GCGGCGCGCG 
AGGGAGGCCG AGATGAAGGC GAAGGAGGCG GAGAGGGAAG CCAGGTGGCA ACAGCCGCAG 
TCCTGCGTCG GCGGCGAACT CTCTTGCATT CCCATAGAAA AGCTTATATC TGTGGACGAC 
ATCGCAGCCA TGGAGTTACG CAGACTTAAT CATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC108628482 ( XM_018030435.2 ) cDNA ORF clone, Ceratina calcarata -> XP_017885924.1 Ceratina calcarata anoctamin-8-like (LOC108628482), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe149339
Clone ID Related Accession (Same CDS sequence)	XM_018030435.1 , XM_018030435.2
Accession Version	XM_018030435.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3396bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-10-16
Organism	Ceratina calcarata
Product	anoctamin-8-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017176396.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Oct 17, 2018 this sequence version replaced XM_018030435.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ceratina calcarata Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAGGCG GCGAGGGGTC CCCGATCAAT CTCCTCGACC AGCATCGTGC ATCGACCACC 
TCCGGCGAGT GCGCGAGTAC AAGTGACGAT GATGAGAAGA CTGCCACGGG CACCGTTTCC 
AGCACGATGA CGACCGTCTT CGCGGGCGAC GGTCTCAGAC GGAGGAAGGT CATACACGCG 
GCCAAAGAAA CCCTCGACAA GGCTTCGCGT CTGCTCAGAC GGAAGATACC ATGCACAGGC 
CACTTGATGA CCCCCCGCCG CCTATGGTTA CAAAAGGTTC CAACGCAGGA ATGCGACGTC 
GTGATGATGT TCCCGAGCGG AGCGAGCGAC GAGACCCTGA TGTGGCTTCT AGGCCGACTT 
CGGGCTGGAA CCCCGGGCTT GGTGGTGCAC GTGCGACACC ATGCCTCTTC AGATAGTTAC 
AGCTTTTACT TAACTGCGCC GTTTAGCGTA CTTTTGAAAG CAGCTGAGGA GGTGCATTTA 
CCGAAGACGC TGCGACAGGA GTTCGGCGGT GGACTGAAGG AGTTCGTTGG CTCCGAGGCG 
AGCTGCTTCG AGGGTAGTGA CGATGAAGCT CAGTTCTTCA CCACCCAGGA GAGGCAATCG 
CTGGTACTCC ACCTGCTGCA CACTCTCAGG GCGGGCCCAC AGGACCTCCA CAGCCTGGCC 
GGGCTCAAGA TGGTCGAAGG TCAAGCAATT ATTCCAAAGT GCATTTCAGC TGGGATCATA 
TCCCAGGTCT TCCCTCTTCA CGAATTGCCT GCGTTGGAGA AACTGCAACG GACATGGGTT 
CGTGCGTTTC TCAGCCCCCA ACCCCTGGAC GACATTTGTA AATACTTCGG AGTCAAGATT 
ACCATGTATT TCGCGTGGCT CGGTCATTAC ACCACCGCTT TGATAGTTCC AGCTGTAGTG 
GGTGCCATAT ATTGGATCGG CATCATCGGT AGGAATCAAG CAGTGGAGGA CGTGGCGTAC 
GTACTGTTCT CTGTGTTCAA CGTAATCTGG GCCACCGTTT ACCTGGAAAC TTGGAAAAGA 
AGGGGTGCCG AACTGGCCTA CAGGTGGGGC ACGTTGGATC AAAGGGACGA CCTACTTCTC 
GAACCTAGGC CCTTATTCAC GGGTACCTTG GAAATTTCAT CGGTGACTGG AAGACTGGAG 
CCGACGTACC CTAGATGGAA GAGGAACATG TTCCGGTATT TCGTCAGCGT GCCCATCATT 
GCAGCGTGCC TGTTCTTCGT TTTCATCGTG ATGATTCTCA GCTTTCAGAT ACAGGATTGG 
TGGGACGCAC GCCTCGAGTC TAGGGGATAC GGTTTCTGGT TGAGCTACGT GCCAAAAGTC 
TTACTCGCGG TGGTGATAGC TTTAATGGAC GAGGCTTACT TCAAGGTTGC CGTATGGTTG 
AACGACATGG AAAACTATCG GCTAGACACC GAGTACGAGA ATCATCTGAT CTACAAGGTG 
GCACTGTTTC AGTTTGTAAA CTCCTTCCTA TCGCTATTTT ACATCGCCTT CTACCTACAG 
GATCAGGAGA GGCTGAAAGA GCAATTGGCC GCTCTGCTGA TAGTCCGGCA GGTGATCGGG 
CATCTGAAAG AGTCTGCAGT GCCGTATCTG ATCGAACAGC TACGACTCGC AAGGTTGAGC 
TTCGAGCTTT TCGGCGCGCT GAGCCCCAGT GAGGCTCGTC CTCCTCCTGG CGAGGACACC 
GAGGAAAATG GGAAGCAGGA GGGCGGCGAT GAGAAGAACG ATCGACCGGA CAGTGCGAAA 
GCGAAGCAGA GAAACGTCAG CCAGGCCGAG CTGGAGAGTT CCCTCTACAG AGTAGGGCAT 
CCCACTAATT CTCTACTTAG TGACGTTACG TTCAAGTACG ACGGGGCGTT TTCAGAGCAT 
CTGGAAATGC TGTCGCAACT GGGATACGTC TGCCTGTTCT CCTCGGCTTT CCCTCTGGCA 
GCGATGTTAG CGTTGCTTGG AAATCTTTTT GAGCTACGAG GCGACGCTTT CAAGCTGTGC 
TTTGTACTTC AGCGACCGTT CGGCCGTCGG GTCTCCAGCA TTGGAACTTG GCAGAACGCC 
ATGGAGGCGA TGGGTCTGGT CGCTATTTTG GTGAACTGCG CGCTGATCGG TCTCAGCGGG 
CAGGTTCAAC GGATGTTCCC GGAGATGTCG GCCACTCAGA CGATTCTGTT GATAGTTGCC 
CTTGAACATA TCATGCTGGC GATACGATTC ATCATAATCT GCGCGATTCC CGACATACCG 
CATTGGGTGG CCACGGAAAT GGCCAAGGTA GAGTTCCTGA GGAGAGAGGC GGTCAGAAGG 
TTATCCTCAA CGCCATCACC GGAGCAGCAA CCGGCTACAG TGATAGGGAG ATTCGTGGTG 
AGTCCAGCAG ACGGTGAGAG CGAAGAGCAA CAATTGTTGT CAGAGGGCAA CGGCGACGCG 
ACGCCCTCCA GCATCCCGGA CACGCCGACG CTGGCGTCGA CCACCCAGAC GCCGAGCAGC 
CCTCCAACTC CTAGTGGTGC ATCCGTGCCG AGGATCTCGG AGACACCCTC CGCGGCTCAA 
ACTCCGGTCA GCTGTGCCAG CAGCGACTTG GGTAGTCCTT TACTGACCGA CAAGAGCATC 
ATAGGCAGCG TCCGAGACAT TCACCACGCG CCTAGGTGTT CCATTCCCGG TGGAGAACGA 
GGAAGGCGTT CAAGGGACTG GCTACAGACT GAATCGGAAG CGTCGGGTGG TGGCGATCAG 
TACAGCCACC ATCTGACGAT CGGTCCTCAC GGTGGTATAG ACTGGGTCCG AAGATTAGGT 
CTCGAGACTG CTGTTCGAAA ATCCAGCGAC TCCGAGATCA GCGGAGCTGG TACCGTCAGT 
GGAAGTGGAG ACGAGTTGAC TCTTCACAGG TCTACTGACT GCATCGTCTC GAAAGAACTC 
GCATCCTCAT CGGATAGCGA TCTCCTCAGA TCCGCCCCGC CATGGTCAGT GGCTCACAGG 
AATCAGAAGT TCCGTTTCTC GCCCGAACGA GAGAAGGAAC GGGAGAAGAC CGAGAAGCAG 
CAGTATCAAC AGTATCAGCG TGAAATTCAA GATCACAAGC AACAAGCAGC GTTGTACGCT 
GAAAAAGAGA AGGAGAAAGA GTCAAGTGCC CTCTCAGACT CGAGCAAAAC TTCCAGTCAG 
GAGGAGGAGA AGCCGTCGAA GGAGGATCGA GAGGCGAAGA AGACCAGGGT GAAGCAGAGT 
TTAATGAAAA GAGCCAGGTC CGTGGCGATC TTCTCGTTAA AGCTGAAGGA GCGGCGCGCG 
AGGGAGGCCG AGATGAAGGC GAAGGAGGCG GAGAGGGAAG CCAGGTGGCA ACAGCCGCAG 
TCCTGCGTCG GCGGCGAACT CTCTTGCATT CCCATAGAAA AGCTTATATC TGTGGACGAC 
ATCGCAGCCA TGGAGTTACG CAGACTTAAT CATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017885924.1
CDS	96..3491
Translation	MPGGEGSPINLLDQHRASTTSGECASTSDDDEKTATGTVSSTMTTVFAGDGLRRRKVIHAAKETLDKASRLLRRKIPCTGHLMTPRRLWLQKVPTQECDVVMMFPSGASDETLMWLLGRLRAGTPGLVVHVRHHASSDSYSFYLTAPFSVLLKAAEEVHLPKTLRQEFGGGLKEFVGSEASCFEGSDDEAQFFTTQERQSLVLHLLHTLRAGPQDLHSLAGLKMVEGQAIIPKCISAGIISQVFPLHELPALEKLQRTWVRAFLSPQPLDDICKYFGVKITMYFAWLGHYTTALIVPAVVGAIYWIGIIGRNQAVEDVAYVLFSVFNVIWATVYLETWKRRGAELAYRWGTLDQRDDLLLEPRPLFTGTLEISSVTGRLEPTYPRWKRNMFRYFVSVPIIAACLFFVFIVMILSFQIQDWWDARLESRGYGFWLSYVPKVLLAVVIALMDEAYFKVAVWLNDMENYRLDTEYENHLIYKVALFQFVNSFLSLFYIAFYLQDQERLKEQLAALLIVRQVIGHLKESAVPYLIEQLRLARLSFELFGALSPSEARPPPGEDTEENGKQEGGDEKNDRPDSAKAKQRNVSQAELESSLYRVGHPTNSLLSDVTFKYDGAFSEHLEMLSQLGYVCLFSSAFPLAAMLALLGNLFELRGDAFKLCFVLQRPFGRRVSSIGTWQNAMEAMGLVAILVNCALIGLSGQVQRMFPEMSATQTILLIVALEHIMLAIRFIIICAIPDIPHWVATEMAKVEFLRREAVRRLSSTPSPEQQPATVIGRFVVSPADGESEEQQLLSEGNGDATPSSIPDTPTLASTTQTPSSPPTPSGASVPRISETPSAAQTPVSCASSDLGSPLLTDKSIIGSVRDIHHAPRCSIPGGERGRRSRDWLQTESEASGGGDQYSHHLTIGPHGGIDWVRRLGLETAVRKSSDSEISGAGTVSGSGDELTLHRSTDCIVSKELASSSDSDLLRSAPPWSVAHRNQKFRFSPEREKEREKTEKQQYQQYQREIQDHKQQAALYAEKEKEKESSALSDSSKTSSQEEEKPSKEDREAKKTRVKQSLMKRARSVAIFSLKLKERRAREAEMKAKEAEREARWQQPQSCVGGELSCIPIEKLISVDDIAAMELRRLNH

Target ORF information:

RefSeq Version	XM_018030435.2
Organism	Ceratina calcarata
Definition	Ceratina calcarata anoctamin-8-like (LOC108628482), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018030435.2

ORF Insert Sequence:

ATGCCAGGCG GCGAGGGGTC CCCGATCAAT CTCCTCGACC AGCATCGTGC ATCGACCACC 
TCCGGCGAGT GCGCGAGTAC AAGTGACGAT GATGAGAAGA CTGCCACGGG CACCGTTTCC 
AGCACGATGA CGACCGTCTT CGCGGGCGAC GGTCTCAGAC GGAGGAAGGT CATACACGCG 
GCCAAAGAAA CCCTCGACAA GGCTTCGCGT CTGCTCAGAC GGAAGATACC ATGCACAGGC 
CACTTGATGA CCCCCCGCCG CCTATGGTTA CAAAAGGTTC CAACGCAGGA ATGCGACGTC 
GTGATGATGT TCCCGAGCGG AGCGAGCGAC GAGACCCTGA TGTGGCTTCT AGGCCGACTT 
CGGGCTGGAA CCCCGGGCTT GGTGGTGCAC GTGCGACACC ATGCCTCTTC AGATAGTTAC 
AGCTTTTACT TAACTGCGCC GTTTAGCGTA CTTTTGAAAG CAGCTGAGGA GGTGCATTTA 
CCGAAGACGC TGCGACAGGA GTTCGGCGGT GGACTGAAGG AGTTCGTTGG CTCCGAGGCG 
AGCTGCTTCG AGGGTAGTGA CGATGAAGCT CAGTTCTTCA CCACCCAGGA GAGGCAATCG 
CTGGTACTCC ACCTGCTGCA CACTCTCAGG GCGGGCCCAC AGGACCTCCA CAGCCTGGCC 
GGGCTCAAGA TGGTCGAAGG TCAAGCAATT ATTCCAAAGT GCATTTCAGC TGGGATCATA 
TCCCAGGTCT TCCCTCTTCA CGAATTGCCT GCGTTGGAGA AACTGCAACG GACATGGGTT 
CGTGCGTTTC TCAGCCCCCA ACCCCTGGAC GACATTTGTA AATACTTCGG AGTCAAGATT 
ACCATGTATT TCGCGTGGCT CGGTCATTAC ACCACCGCTT TGATAGTTCC AGCTGTAGTG 
GGTGCCATAT ATTGGATCGG CATCATCGGT AGGAATCAAG CAGTGGAGGA CGTGGCGTAC 
GTACTGTTCT CTGTGTTCAA CGTAATCTGG GCCACCGTTT ACCTGGAAAC TTGGAAAAGA 
AGGGGTGCCG AACTGGCCTA CAGGTGGGGC ACGTTGGATC AAAGGGACGA CCTACTTCTC 
GAACCTAGGC CCTTATTCAC GGGTACCTTG GAAATTTCAT CGGTGACTGG AAGACTGGAG 
CCGACGTACC CTAGATGGAA GAGGAACATG TTCCGGTATT TCGTCAGCGT GCCCATCATT 
GCAGCGTGCC TGTTCTTCGT TTTCATCGTG ATGATTCTCA GCTTTCAGAT ACAGGATTGG 
TGGGACGCAC GCCTCGAGTC TAGGGGATAC GGTTTCTGGT TGAGCTACGT GCCAAAAGTC 
TTACTCGCGG TGGTGATAGC TTTAATGGAC GAGGCTTACT TCAAGGTTGC CGTATGGTTG 
AACGACATGG AAAACTATCG GCTAGACACC GAGTACGAGA ATCATCTGAT CTACAAGGTG 
GCACTGTTTC AGTTTGTAAA CTCCTTCCTA TCGCTATTTT ACATCGCCTT CTACCTACAG 
GATCAGGAGA GGCTGAAAGA GCAATTGGCC GCTCTGCTGA TAGTCCGGCA GGTGATCGGG 
CATCTGAAAG AGTCTGCAGT GCCGTATCTG ATCGAACAGC TACGACTCGC AAGGTTGAGC 
TTCGAGCTTT TCGGCGCGCT GAGCCCCAGT GAGGCTCGTC CTCCTCCTGG CGAGGACACC 
GAGGAAAATG GGAAGCAGGA GGGCGGCGAT GAGAAGAACG ATCGACCGGA CAGTGCGAAA 
GCGAAGCAGA GAAACGTCAG CCAGGCCGAG CTGGAGAGTT CCCTCTACAG AGTAGGGCAT 
CCCACTAATT CTCTACTTAG TGACGTTACG TTCAAGTACG ACGGGGCGTT TTCAGAGCAT 
CTGGAAATGC TGTCGCAACT GGGATACGTC TGCCTGTTCT CCTCGGCTTT CCCTCTGGCA 
GCGATGTTAG CGTTGCTTGG AAATCTTTTT GAGCTACGAG GCGACGCTTT CAAGCTGTGC 
TTTGTACTTC AGCGACCGTT CGGCCGTCGG GTCTCCAGCA TTGGAACTTG GCAGAACGCC 
ATGGAGGCGA TGGGTCTGGT CGCTATTTTG GTGAACTGCG CGCTGATCGG TCTCAGCGGG 
CAGGTTCAAC GGATGTTCCC GGAGATGTCG GCCACTCAGA CGATTCTGTT GATAGTTGCC 
CTTGAACATA TCATGCTGGC GATACGATTC ATCATAATCT GCGCGATTCC CGACATACCG 
CATTGGGTGG CCACGGAAAT GGCCAAGGTA GAGTTCCTGA GGAGAGAGGC GGTCAGAAGG 
TTATCCTCAA CGCCATCACC GGAGCAGCAA CCGGCTACAG TGATAGGGAG ATTCGTGGTG 
AGTCCAGCAG ACGGTGAGAG CGAAGAGCAA CAATTGTTGT CAGAGGGCAA CGGCGACGCG 
ACGCCCTCCA GCATCCCGGA CACGCCGACG CTGGCGTCGA CCACCCAGAC GCCGAGCAGC 
CCTCCAACTC CTAGTGGTGC ATCCGTGCCG AGGATCTCGG AGACACCCTC CGCGGCTCAA 
ACTCCGGTCA GCTGTGCCAG CAGCGACTTG GGTAGTCCTT TACTGACCGA CAAGAGCATC 
ATAGGCAGCG TCCGAGACAT TCACCACGCG CCTAGGTGTT CCATTCCCGG TGGAGAACGA 
GGAAGGCGTT CAAGGGACTG GCTACAGACT GAATCGGAAG CGTCGGGTGG TGGCGATCAG 
TACAGCCACC ATCTGACGAT CGGTCCTCAC GGTGGTATAG ACTGGGTCCG AAGATTAGGT 
CTCGAGACTG CTGTTCGAAA ATCCAGCGAC TCCGAGATCA GCGGAGCTGG TACCGTCAGT 
GGAAGTGGAG ACGAGTTGAC TCTTCACAGG TCTACTGACT GCATCGTCTC GAAAGAACTC 
GCATCCTCAT CGGATAGCGA TCTCCTCAGA TCCGCCCCGC CATGGTCAGT GGCTCACAGG 
AATCAGAAGT TCCGTTTCTC GCCCGAACGA GAGAAGGAAC GGGAGAAGAC CGAGAAGCAG 
CAGTATCAAC AGTATCAGCG TGAAATTCAA GATCACAAGC AACAAGCAGC GTTGTACGCT 
GAAAAAGAGA AGGAGAAAGA GTCAAGTGCC CTCTCAGACT CGAGCAAAAC TTCCAGTCAG 
GAGGAGGAGA AGCCGTCGAA GGAGGATCGA GAGGCGAAGA AGACCAGGGT GAAGCAGAGT 
TTAATGAAAA GAGCCAGGTC CGTGGCGATC TTCTCGTTAA AGCTGAAGGA GCGGCGCGCG 
AGGGAGGCCG AGATGAAGGC GAAGGAGGCG GAGAGGGAAG CCAGGTGGCA ACAGCCGCAG 
TCCTGCGTCG GCGGCGAACT CTCTTGCATT CCCATAGAAA AGCTTATATC TGTGGACGAC 
ATCGCAGCCA TGGAGTTACG CAGACTTAAT CATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC108628482 ( XM_018030436.1 ) cDNA ORF clone, Ceratina calcarata -> XP_017885925.1 Ceratina calcarata anoctamin-8-like (LOC108628482), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCe149340
Clone ID Related Accession (Same CDS sequence)	XM_018030436.1 , XM_018030436.2
Accession Version	XM_018030436.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3375bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-08-30
Organism	Ceratina calcarata
Product	anoctamin-8-like isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017176396.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ceratina calcarata Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAGGCG GCGAGGGGTC CCCGATCAAT CTCCTCGACC AGCATCGTGC ATCGACCACC 
TCCGGCGAGT GCGCGAGTAC AAGTGACGAT GATGAGAAGA CTGCCACGGG CACCGTTTCC 
AGCACGATGA CGACCGTCTT CGCGGGCGAC GGTCTCAGAC GGAGGAAGGT CATACACGCG 
GCCAAAGAAA CCCTCGACAA GGCTTCGCGT CTGCTCAGAC GGAAGATACC ATGCACAGGC 
CACTTGATGA CCCCCCGCCG CCTATGGTTA CAAAAGGTTC CAACGCAGGA ATGCGACGTC 
GTGATGATGT TCCCGAGCGG AGCGAGCGAC GAGACCCTGA TGTGGCTTCT AGGCCGACTT 
CGGGCTGGAA CCCCGGGCTT GGTGGTGCAC GTGCGACACC ATGCCTCTTC AGATAGTTAC 
AGCTTTTACT TAACTGCGCC GTTTAGCGTA CTTTTGAAAG CAGCTGAGGA GGTGCATTTA 
CCGAAGACGC TGCGACAGGA GTTCGGCGGT GGACTGAAGG AGTTCGTTGG CTCCGAGGCG 
AGCTGCTTCG AGGGTAGTGA CGATGAAGCT CAGTTCTTCA CCACCCAGGA GAGGCAATCG 
CTGGTACTCC ACCTGCTGCA CACTCTCAGG GCGGGCCCAC AGGACCTCCA CAGCCTGGCC 
GGGCTCAAGA TGGTCGAAGG TCAAGCAATT ATTCCAAAGT GCATTTCAGC TGGGATCATA 
TCCCAGGTCT TCCCTCTTCA CGAATTGCCT GCGTTGGAGA AACTGCAACG GACATGGGTT 
CGTGCGTTTC TCAGCCCCCA ACCCCTGGAC GACATTTGTA AATACTTCGG AGTCAAGATT 
ACCATGTATT TCGCGTGGCT CGGTCATTAC ACCACCGCTT TGATAGTTCC AGCTGTAGTG 
GGTGCCATAT ATTGGATCGG CATCATCGGT AGGAATCAAG CAGTGGAGGA CGTGGCGTAC 
GTACTGTTCT CTGTGTTCAA CGTAATCTGG GCCACCGTTT ACCTGGAAAC TTGGAAAAGA 
AGGGGTGCCG AACTGGCCTA CAGGTGGGGC ACGTTGGATC AAAGGGACGA CCTACTTCTC 
GAACCTAGGC CCTTATTCAC GGGTACCTTG GAAATTTCAT CGGTGACTGG AAGACTGGAG 
CCGACGTACC CTAGATGGAA GAGGAACATG TTCCGGTATT TCGTCAGCGT GCCCATCATT 
GCAGCGTGCC TGTTCTTCGT TTTCATCGTG ATGATTCTCA GCTTTCAGAT ACAGGATTGG 
TGGGACGCAC GCCTCGAGTC TAGGGGATAC GGTTTCTGGT TGAGCTACGT GCCAAAAGTC 
TTACTCGCGG TGGTGATAGC TTTAATGGAC GAGGCTTACT TCAAGGTTGC CGTATGGTTG 
AACGACATGG AAAACTATCG GCTAGACACC GAGTACGAGA ATCATCTGAT CTACAAGGTG 
GCACTGTTTC AGTTTGTAAA CTCCTTCCTA TCGCTATTTT ACATCGCCTT CTACCTACAG 
GATCAGGAGA GGCTGAAAGA GCAATTGGCC GCTCTGCTGA TAGTCCGGCA GGTGATCGGG 
CATCTGAAAG AGTCTGCAGT GCCGTATCTG ATCGAACAGC TACGACTCGC AAGGTTGAGC 
TTCGAGCTTT TCGGCGCGCT GAGCCCCAGT GAGGCTCGTC CTCCTCCTGG CGAGGACACC 
GAGGAAAATG GGAAGCAGGA GGGCGGCGAT GAGAAGAACG ATCGACCGGA CAGTGCGAAA 
GCGAAGCAGA GAAACGTCAG CCAGGCCGAG CTGGAGAGTT CCCTCTACAG AGTAGGGCAT 
CCCACTAATT CTCTACTTAG TGACGTTACG TTCAAGTACG ACGGGGCGTT TTCAGAGCAT 
CTGGAAATGC TGTCGCAACT GGGATACGTC TGCCTGTTCT CCTCGGCTTT CCCTCTGGCA 
GCGATGTTAG CGTTGCTTGG AAATCTTTTT GAGCTACGAG GCGACGCTTT CAAGCTGTGC 
TTTGTACTTC AGCGACCGTT CGGCCGTCGG GTCTCCAGCA TTGGAACTTG GCAGAACGCC 
ATGGAGGCGA TGGGTCTGGT CGCTATTTTG GTGAACTGCG CGCTGATCGG TCTCAGCGGG 
CAGGTTCAAC GGATGTTCCC GGAGATGTCG GCCACTCAGA CGATTCTGTT GATAGTTGCC 
CTTGAACATA TCATGCTGGC GATACGATTC ATCATAATCT GCGCGATTCC CGACATACCG 
CATTGGGTGG CCACGGAAAT GGCCAAGGTA GAGTTCCTGA GGAGAGAGGC GGTCAGAAGG 
TTATCCTCAA CGCCATCACC GGAGCAGCAA CCGGCTACAG TGATAGCAGA CGGTGAGAGC 
GAAGAGCAAC AATTGTTGTC AGAGGGCAAC GGCGACGCGA CGCCCTCCAG CATCCCGGAC 
ACGCCGACGC TGGCGTCGAC CACCCAGACG CCGAGCAGCC CTCCAACTCC TAGTGGTGCA 
TCCGTGCCGA GGATCTCGGA GACACCCTCC GCGGCTCAAA CTCCGGTCAG CTGTGCCAGC 
AGCGACTTGG GTAGTCCTTT ACTGACCGAC AAGAGCATCA TAGGCAGCGT CCGAGACATT 
CACCACGCGC CTAGGTGTTC CATTCCCGGT GGAGAACGAG GAAGGCGTTC AAGGGACTGG 
CTACAGACTG AATCGGAAGC GTCGGGTGGT GGCGATCAGT ACAGCCACCA TCTGACGATC 
GGTCCTCACG GTGGTATAGA CTGGGTCCGA AGATTAGGTC TCGAGACTGC TGTTCGAAAA 
TCCAGCGACT CCGAGATCAG CGGAGCTGGT ACCGTCAGTG GAAGTGGAGA CGAGTTGACT 
CTTCACAGGT CTACTGACTG CATCGTCTCG AAAGAACTCG CATCCTCATC GGATAGCGAT 
CTCCTCAGAT CCGCCCCGCC ATGGTCAGTG GCTCACAGGA ATCAGAAGTT CCGTTTCTCG 
CCCGAACGAG AGAAGGAACG GGAGAAGACC GAGAAGCAGC AGTATCAACA GTATCAGCGT 
GAAATTCAAG ATCACAAGCA ACAAGCAGCG TTGTACGCTG AAAAAGAGAA GGAGAAAGAG 
TCAAGTGCCC TCTCAGACTC GAGCAAAACT TCCAGTCAGG AGGAGGAGAA GCCGTCGAAG 
GAGGATCGAG AGGCGAAGAA GACCAGGGTG AAGCAGAGTT TAATGAAAAG AGCCAGGTCC 
GTGGCGATCT TCTCGTTAAA GCTGAAGGAG CGGCGCGCGA GGGAGGCCGA GATGAAGGCG 
AAGGAGGCGG AGAGGGAAGC CAGGTGGCAA CAGCCGCAGT CCTGCGTCGG CGGCGAACTC 
TCTTGCATTC CCATAGAAAA GCTTATATCT GTGGACGACA TCGCAGCCAT GGAGTTACGC 
AGACTTAATC ATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017885925.1
CDS	64..3438
Translation	MPGGEGSPINLLDQHRASTTSGECASTSDDDEKTATGTVSSTMTTVFAGDGLRRRKVIHAAKETLDKASRLLRRKIPCTGHLMTPRRLWLQKVPTQECDVVMMFPSGASDETLMWLLGRLRAGTPGLVVHVRHHASSDSYSFYLTAPFSVLLKAAEEVHLPKTLRQEFGGGLKEFVGSEASCFEGSDDEAQFFTTQERQSLVLHLLHTLRAGPQDLHSLAGLKMVEGQAIIPKCISAGIISQVFPLHELPALEKLQRTWVRAFLSPQPLDDICKYFGVKITMYFAWLGHYTTALIVPAVVGAIYWIGIIGRNQAVEDVAYVLFSVFNVIWATVYLETWKRRGAELAYRWGTLDQRDDLLLEPRPLFTGTLEISSVTGRLEPTYPRWKRNMFRYFVSVPIIAACLFFVFIVMILSFQIQDWWDARLESRGYGFWLSYVPKVLLAVVIALMDEAYFKVAVWLNDMENYRLDTEYENHLIYKVALFQFVNSFLSLFYIAFYLQDQERLKEQLAALLIVRQVIGHLKESAVPYLIEQLRLARLSFELFGALSPSEARPPPGEDTEENGKQEGGDEKNDRPDSAKAKQRNVSQAELESSLYRVGHPTNSLLSDVTFKYDGAFSEHLEMLSQLGYVCLFSSAFPLAAMLALLGNLFELRGDAFKLCFVLQRPFGRRVSSIGTWQNAMEAMGLVAILVNCALIGLSGQVQRMFPEMSATQTILLIVALEHIMLAIRFIIICAIPDIPHWVATEMAKVEFLRREAVRRLSSTPSPEQQPATVIADGESEEQQLLSEGNGDATPSSIPDTPTLASTTQTPSSPPTPSGASVPRISETPSAAQTPVSCASSDLGSPLLTDKSIIGSVRDIHHAPRCSIPGGERGRRSRDWLQTESEASGGGDQYSHHLTIGPHGGIDWVRRLGLETAVRKSSDSEISGAGTVSGSGDELTLHRSTDCIVSKELASSSDSDLLRSAPPWSVAHRNQKFRFSPEREKEREKTEKQQYQQYQREIQDHKQQAALYAEKEKEKESSALSDSSKTSSQEEEKPSKEDREAKKTRVKQSLMKRARSVAIFSLKLKERRAREAEMKAKEAEREARWQQPQSCVGGELSCIPIEKLISVDDIAAMELRRLNH

Target ORF information:

RefSeq Version	XM_018030436.1
Organism	Ceratina calcarata
Definition	Ceratina calcarata anoctamin-8-like (LOC108628482), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018030436.1

ORF Insert Sequence:

ATGCCAGGCG GCGAGGGGTC CCCGATCAAT CTCCTCGACC AGCATCGTGC ATCGACCACC 
TCCGGCGAGT GCGCGAGTAC AAGTGACGAT GATGAGAAGA CTGCCACGGG CACCGTTTCC 
AGCACGATGA CGACCGTCTT CGCGGGCGAC GGTCTCAGAC GGAGGAAGGT CATACACGCG 
GCCAAAGAAA CCCTCGACAA GGCTTCGCGT CTGCTCAGAC GGAAGATACC ATGCACAGGC 
CACTTGATGA CCCCCCGCCG CCTATGGTTA CAAAAGGTTC CAACGCAGGA ATGCGACGTC 
GTGATGATGT TCCCGAGCGG AGCGAGCGAC GAGACCCTGA TGTGGCTTCT AGGCCGACTT 
CGGGCTGGAA CCCCGGGCTT GGTGGTGCAC GTGCGACACC ATGCCTCTTC AGATAGTTAC 
AGCTTTTACT TAACTGCGCC GTTTAGCGTA CTTTTGAAAG CAGCTGAGGA GGTGCATTTA 
CCGAAGACGC TGCGACAGGA GTTCGGCGGT GGACTGAAGG AGTTCGTTGG CTCCGAGGCG 
AGCTGCTTCG AGGGTAGTGA CGATGAAGCT CAGTTCTTCA CCACCCAGGA GAGGCAATCG 
CTGGTACTCC ACCTGCTGCA CACTCTCAGG GCGGGCCCAC AGGACCTCCA CAGCCTGGCC 
GGGCTCAAGA TGGTCGAAGG TCAAGCAATT ATTCCAAAGT GCATTTCAGC TGGGATCATA 
TCCCAGGTCT TCCCTCTTCA CGAATTGCCT GCGTTGGAGA AACTGCAACG GACATGGGTT 
CGTGCGTTTC TCAGCCCCCA ACCCCTGGAC GACATTTGTA AATACTTCGG AGTCAAGATT 
ACCATGTATT TCGCGTGGCT CGGTCATTAC ACCACCGCTT TGATAGTTCC AGCTGTAGTG 
GGTGCCATAT ATTGGATCGG CATCATCGGT AGGAATCAAG CAGTGGAGGA CGTGGCGTAC 
GTACTGTTCT CTGTGTTCAA CGTAATCTGG GCCACCGTTT ACCTGGAAAC TTGGAAAAGA 
AGGGGTGCCG AACTGGCCTA CAGGTGGGGC ACGTTGGATC AAAGGGACGA CCTACTTCTC 
GAACCTAGGC CCTTATTCAC GGGTACCTTG GAAATTTCAT CGGTGACTGG AAGACTGGAG 
CCGACGTACC CTAGATGGAA GAGGAACATG TTCCGGTATT TCGTCAGCGT GCCCATCATT 
GCAGCGTGCC TGTTCTTCGT TTTCATCGTG ATGATTCTCA GCTTTCAGAT ACAGGATTGG 
TGGGACGCAC GCCTCGAGTC TAGGGGATAC GGTTTCTGGT TGAGCTACGT GCCAAAAGTC 
TTACTCGCGG TGGTGATAGC TTTAATGGAC GAGGCTTACT TCAAGGTTGC CGTATGGTTG 
AACGACATGG AAAACTATCG GCTAGACACC GAGTACGAGA ATCATCTGAT CTACAAGGTG 
GCACTGTTTC AGTTTGTAAA CTCCTTCCTA TCGCTATTTT ACATCGCCTT CTACCTACAG 
GATCAGGAGA GGCTGAAAGA GCAATTGGCC GCTCTGCTGA TAGTCCGGCA GGTGATCGGG 
CATCTGAAAG AGTCTGCAGT GCCGTATCTG ATCGAACAGC TACGACTCGC AAGGTTGAGC 
TTCGAGCTTT TCGGCGCGCT GAGCCCCAGT GAGGCTCGTC CTCCTCCTGG CGAGGACACC 
GAGGAAAATG GGAAGCAGGA GGGCGGCGAT GAGAAGAACG ATCGACCGGA CAGTGCGAAA 
GCGAAGCAGA GAAACGTCAG CCAGGCCGAG CTGGAGAGTT CCCTCTACAG AGTAGGGCAT 
CCCACTAATT CTCTACTTAG TGACGTTACG TTCAAGTACG ACGGGGCGTT TTCAGAGCAT 
CTGGAAATGC TGTCGCAACT GGGATACGTC TGCCTGTTCT CCTCGGCTTT CCCTCTGGCA 
GCGATGTTAG CGTTGCTTGG AAATCTTTTT GAGCTACGAG GCGACGCTTT CAAGCTGTGC 
TTTGTACTTC AGCGACCGTT CGGCCGTCGG GTCTCCAGCA TTGGAACTTG GCAGAACGCC 
ATGGAGGCGA TGGGTCTGGT CGCTATTTTG GTGAACTGCG CGCTGATCGG TCTCAGCGGG 
CAGGTTCAAC GGATGTTCCC GGAGATGTCG GCCACTCAGA CGATTCTGTT GATAGTTGCC 
CTTGAACATA TCATGCTGGC GATACGATTC ATCATAATCT GCGCGATTCC CGACATACCG 
CATTGGGTGG CCACGGAAAT GGCCAAGGTA GAGTTCCTGA GGAGAGAGGC GGTCAGAAGG 
TTATCCTCAA CGCCATCACC GGAGCAGCAA CCGGCTACAG TGATAGCAGA CGGTGAGAGC 
GAAGAGCAAC AATTGTTGTC AGAGGGCAAC GGCGACGCGA CGCCCTCCAG CATCCCGGAC 
ACGCCGACGC TGGCGTCGAC CACCCAGACG CCGAGCAGCC CTCCAACTCC TAGTGGTGCA 
TCCGTGCCGA GGATCTCGGA GACACCCTCC GCGGCTCAAA CTCCGGTCAG CTGTGCCAGC 
AGCGACTTGG GTAGTCCTTT ACTGACCGAC AAGAGCATCA TAGGCAGCGT CCGAGACATT 
CACCACGCGC CTAGGTGTTC CATTCCCGGT GGAGAACGAG GAAGGCGTTC AAGGGACTGG 
CTACAGACTG AATCGGAAGC GTCGGGTGGT GGCGATCAGT ACAGCCACCA TCTGACGATC 
GGTCCTCACG GTGGTATAGA CTGGGTCCGA AGATTAGGTC TCGAGACTGC TGTTCGAAAA 
TCCAGCGACT CCGAGATCAG CGGAGCTGGT ACCGTCAGTG GAAGTGGAGA CGAGTTGACT 
CTTCACAGGT CTACTGACTG CATCGTCTCG AAAGAACTCG CATCCTCATC GGATAGCGAT 
CTCCTCAGAT CCGCCCCGCC ATGGTCAGTG GCTCACAGGA ATCAGAAGTT CCGTTTCTCG 
CCCGAACGAG AGAAGGAACG GGAGAAGACC GAGAAGCAGC AGTATCAACA GTATCAGCGT 
GAAATTCAAG ATCACAAGCA ACAAGCAGCG TTGTACGCTG AAAAAGAGAA GGAGAAAGAG 
TCAAGTGCCC TCTCAGACTC GAGCAAAACT TCCAGTCAGG AGGAGGAGAA GCCGTCGAAG 
GAGGATCGAG AGGCGAAGAA GACCAGGGTG AAGCAGAGTT TAATGAAAAG AGCCAGGTCC 
GTGGCGATCT TCTCGTTAAA GCTGAAGGAG CGGCGCGCGA GGGAGGCCGA GATGAAGGCG 
AAGGAGGCGG AGAGGGAAGC CAGGTGGCAA CAGCCGCAGT CCTGCGTCGG CGGCGAACTC 
TCTTGCATTC CCATAGAAAA GCTTATATCT GTGGACGACA TCGCAGCCAT GGAGTTACGC 
AGACTTAATC ATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC108628482 ( XM_018030436.2 ) cDNA ORF clone, Ceratina calcarata -> XP_017885925.1 Ceratina calcarata anoctamin-8-like (LOC108628482), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCe149340
Clone ID Related Accession (Same CDS sequence)	XM_018030436.1 , XM_018030436.2
Accession Version	XM_018030436.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3375bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-10-16
Organism	Ceratina calcarata
Product	anoctamin-8-like isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017176396.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Oct 17, 2018 this sequence version replaced XM_018030436.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ceratina calcarata Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAGGCG GCGAGGGGTC CCCGATCAAT CTCCTCGACC AGCATCGTGC ATCGACCACC 
TCCGGCGAGT GCGCGAGTAC AAGTGACGAT GATGAGAAGA CTGCCACGGG CACCGTTTCC 
AGCACGATGA CGACCGTCTT CGCGGGCGAC GGTCTCAGAC GGAGGAAGGT CATACACGCG 
GCCAAAGAAA CCCTCGACAA GGCTTCGCGT CTGCTCAGAC GGAAGATACC ATGCACAGGC 
CACTTGATGA CCCCCCGCCG CCTATGGTTA CAAAAGGTTC CAACGCAGGA ATGCGACGTC 
GTGATGATGT TCCCGAGCGG AGCGAGCGAC GAGACCCTGA TGTGGCTTCT AGGCCGACTT 
CGGGCTGGAA CCCCGGGCTT GGTGGTGCAC GTGCGACACC ATGCCTCTTC AGATAGTTAC 
AGCTTTTACT TAACTGCGCC GTTTAGCGTA CTTTTGAAAG CAGCTGAGGA GGTGCATTTA 
CCGAAGACGC TGCGACAGGA GTTCGGCGGT GGACTGAAGG AGTTCGTTGG CTCCGAGGCG 
AGCTGCTTCG AGGGTAGTGA CGATGAAGCT CAGTTCTTCA CCACCCAGGA GAGGCAATCG 
CTGGTACTCC ACCTGCTGCA CACTCTCAGG GCGGGCCCAC AGGACCTCCA CAGCCTGGCC 
GGGCTCAAGA TGGTCGAAGG TCAAGCAATT ATTCCAAAGT GCATTTCAGC TGGGATCATA 
TCCCAGGTCT TCCCTCTTCA CGAATTGCCT GCGTTGGAGA AACTGCAACG GACATGGGTT 
CGTGCGTTTC TCAGCCCCCA ACCCCTGGAC GACATTTGTA AATACTTCGG AGTCAAGATT 
ACCATGTATT TCGCGTGGCT CGGTCATTAC ACCACCGCTT TGATAGTTCC AGCTGTAGTG 
GGTGCCATAT ATTGGATCGG CATCATCGGT AGGAATCAAG CAGTGGAGGA CGTGGCGTAC 
GTACTGTTCT CTGTGTTCAA CGTAATCTGG GCCACCGTTT ACCTGGAAAC TTGGAAAAGA 
AGGGGTGCCG AACTGGCCTA CAGGTGGGGC ACGTTGGATC AAAGGGACGA CCTACTTCTC 
GAACCTAGGC CCTTATTCAC GGGTACCTTG GAAATTTCAT CGGTGACTGG AAGACTGGAG 
CCGACGTACC CTAGATGGAA GAGGAACATG TTCCGGTATT TCGTCAGCGT GCCCATCATT 
GCAGCGTGCC TGTTCTTCGT TTTCATCGTG ATGATTCTCA GCTTTCAGAT ACAGGATTGG 
TGGGACGCAC GCCTCGAGTC TAGGGGATAC GGTTTCTGGT TGAGCTACGT GCCAAAAGTC 
TTACTCGCGG TGGTGATAGC TTTAATGGAC GAGGCTTACT TCAAGGTTGC CGTATGGTTG 
AACGACATGG AAAACTATCG GCTAGACACC GAGTACGAGA ATCATCTGAT CTACAAGGTG 
GCACTGTTTC AGTTTGTAAA CTCCTTCCTA TCGCTATTTT ACATCGCCTT CTACCTACAG 
GATCAGGAGA GGCTGAAAGA GCAATTGGCC GCTCTGCTGA TAGTCCGGCA GGTGATCGGG 
CATCTGAAAG AGTCTGCAGT GCCGTATCTG ATCGAACAGC TACGACTCGC AAGGTTGAGC 
TTCGAGCTTT TCGGCGCGCT GAGCCCCAGT GAGGCTCGTC CTCCTCCTGG CGAGGACACC 
GAGGAAAATG GGAAGCAGGA GGGCGGCGAT GAGAAGAACG ATCGACCGGA CAGTGCGAAA 
GCGAAGCAGA GAAACGTCAG CCAGGCCGAG CTGGAGAGTT CCCTCTACAG AGTAGGGCAT 
CCCACTAATT CTCTACTTAG TGACGTTACG TTCAAGTACG ACGGGGCGTT TTCAGAGCAT 
CTGGAAATGC TGTCGCAACT GGGATACGTC TGCCTGTTCT CCTCGGCTTT CCCTCTGGCA 
GCGATGTTAG CGTTGCTTGG AAATCTTTTT GAGCTACGAG GCGACGCTTT CAAGCTGTGC 
TTTGTACTTC AGCGACCGTT CGGCCGTCGG GTCTCCAGCA TTGGAACTTG GCAGAACGCC 
ATGGAGGCGA TGGGTCTGGT CGCTATTTTG GTGAACTGCG CGCTGATCGG TCTCAGCGGG 
CAGGTTCAAC GGATGTTCCC GGAGATGTCG GCCACTCAGA CGATTCTGTT GATAGTTGCC 
CTTGAACATA TCATGCTGGC GATACGATTC ATCATAATCT GCGCGATTCC CGACATACCG 
CATTGGGTGG CCACGGAAAT GGCCAAGGTA GAGTTCCTGA GGAGAGAGGC GGTCAGAAGG 
TTATCCTCAA CGCCATCACC GGAGCAGCAA CCGGCTACAG TGATAGCAGA CGGTGAGAGC 
GAAGAGCAAC AATTGTTGTC AGAGGGCAAC GGCGACGCGA CGCCCTCCAG CATCCCGGAC 
ACGCCGACGC TGGCGTCGAC CACCCAGACG CCGAGCAGCC CTCCAACTCC TAGTGGTGCA 
TCCGTGCCGA GGATCTCGGA GACACCCTCC GCGGCTCAAA CTCCGGTCAG CTGTGCCAGC 
AGCGACTTGG GTAGTCCTTT ACTGACCGAC AAGAGCATCA TAGGCAGCGT CCGAGACATT 
CACCACGCGC CTAGGTGTTC CATTCCCGGT GGAGAACGAG GAAGGCGTTC AAGGGACTGG 
CTACAGACTG AATCGGAAGC GTCGGGTGGT GGCGATCAGT ACAGCCACCA TCTGACGATC 
GGTCCTCACG GTGGTATAGA CTGGGTCCGA AGATTAGGTC TCGAGACTGC TGTTCGAAAA 
TCCAGCGACT CCGAGATCAG CGGAGCTGGT ACCGTCAGTG GAAGTGGAGA CGAGTTGACT 
CTTCACAGGT CTACTGACTG CATCGTCTCG AAAGAACTCG CATCCTCATC GGATAGCGAT 
CTCCTCAGAT CCGCCCCGCC ATGGTCAGTG GCTCACAGGA ATCAGAAGTT CCGTTTCTCG 
CCCGAACGAG AGAAGGAACG GGAGAAGACC GAGAAGCAGC AGTATCAACA GTATCAGCGT 
GAAATTCAAG ATCACAAGCA ACAAGCAGCG TTGTACGCTG AAAAAGAGAA GGAGAAAGAG 
TCAAGTGCCC TCTCAGACTC GAGCAAAACT TCCAGTCAGG AGGAGGAGAA GCCGTCGAAG 
GAGGATCGAG AGGCGAAGAA GACCAGGGTG AAGCAGAGTT TAATGAAAAG AGCCAGGTCC 
GTGGCGATCT TCTCGTTAAA GCTGAAGGAG CGGCGCGCGA GGGAGGCCGA GATGAAGGCG 
AAGGAGGCGG AGAGGGAAGC CAGGTGGCAA CAGCCGCAGT CCTGCGTCGG CGGCGAACTC 
TCTTGCATTC CCATAGAAAA GCTTATATCT GTGGACGACA TCGCAGCCAT GGAGTTACGC 
AGACTTAATC ATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017885925.1
CDS	96..3470
Translation	MPGGEGSPINLLDQHRASTTSGECASTSDDDEKTATGTVSSTMTTVFAGDGLRRRKVIHAAKETLDKASRLLRRKIPCTGHLMTPRRLWLQKVPTQECDVVMMFPSGASDETLMWLLGRLRAGTPGLVVHVRHHASSDSYSFYLTAPFSVLLKAAEEVHLPKTLRQEFGGGLKEFVGSEASCFEGSDDEAQFFTTQERQSLVLHLLHTLRAGPQDLHSLAGLKMVEGQAIIPKCISAGIISQVFPLHELPALEKLQRTWVRAFLSPQPLDDICKYFGVKITMYFAWLGHYTTALIVPAVVGAIYWIGIIGRNQAVEDVAYVLFSVFNVIWATVYLETWKRRGAELAYRWGTLDQRDDLLLEPRPLFTGTLEISSVTGRLEPTYPRWKRNMFRYFVSVPIIAACLFFVFIVMILSFQIQDWWDARLESRGYGFWLSYVPKVLLAVVIALMDEAYFKVAVWLNDMENYRLDTEYENHLIYKVALFQFVNSFLSLFYIAFYLQDQERLKEQLAALLIVRQVIGHLKESAVPYLIEQLRLARLSFELFGALSPSEARPPPGEDTEENGKQEGGDEKNDRPDSAKAKQRNVSQAELESSLYRVGHPTNSLLSDVTFKYDGAFSEHLEMLSQLGYVCLFSSAFPLAAMLALLGNLFELRGDAFKLCFVLQRPFGRRVSSIGTWQNAMEAMGLVAILVNCALIGLSGQVQRMFPEMSATQTILLIVALEHIMLAIRFIIICAIPDIPHWVATEMAKVEFLRREAVRRLSSTPSPEQQPATVIADGESEEQQLLSEGNGDATPSSIPDTPTLASTTQTPSSPPTPSGASVPRISETPSAAQTPVSCASSDLGSPLLTDKSIIGSVRDIHHAPRCSIPGGERGRRSRDWLQTESEASGGGDQYSHHLTIGPHGGIDWVRRLGLETAVRKSSDSEISGAGTVSGSGDELTLHRSTDCIVSKELASSSDSDLLRSAPPWSVAHRNQKFRFSPEREKEREKTEKQQYQQYQREIQDHKQQAALYAEKEKEKESSALSDSSKTSSQEEEKPSKEDREAKKTRVKQSLMKRARSVAIFSLKLKERRAREAEMKAKEAEREARWQQPQSCVGGELSCIPIEKLISVDDIAAMELRRLNH

Target ORF information:

RefSeq Version	XM_018030436.2
Organism	Ceratina calcarata
Definition	Ceratina calcarata anoctamin-8-like (LOC108628482), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018030436.2

ORF Insert Sequence:

ATGCCAGGCG GCGAGGGGTC CCCGATCAAT CTCCTCGACC AGCATCGTGC ATCGACCACC 
TCCGGCGAGT GCGCGAGTAC AAGTGACGAT GATGAGAAGA CTGCCACGGG CACCGTTTCC 
AGCACGATGA CGACCGTCTT CGCGGGCGAC GGTCTCAGAC GGAGGAAGGT CATACACGCG 
GCCAAAGAAA CCCTCGACAA GGCTTCGCGT CTGCTCAGAC GGAAGATACC ATGCACAGGC 
CACTTGATGA CCCCCCGCCG CCTATGGTTA CAAAAGGTTC CAACGCAGGA ATGCGACGTC 
GTGATGATGT TCCCGAGCGG AGCGAGCGAC GAGACCCTGA TGTGGCTTCT AGGCCGACTT 
CGGGCTGGAA CCCCGGGCTT GGTGGTGCAC GTGCGACACC ATGCCTCTTC AGATAGTTAC 
AGCTTTTACT TAACTGCGCC GTTTAGCGTA CTTTTGAAAG CAGCTGAGGA GGTGCATTTA 
CCGAAGACGC TGCGACAGGA GTTCGGCGGT GGACTGAAGG AGTTCGTTGG CTCCGAGGCG 
AGCTGCTTCG AGGGTAGTGA CGATGAAGCT CAGTTCTTCA CCACCCAGGA GAGGCAATCG 
CTGGTACTCC ACCTGCTGCA CACTCTCAGG GCGGGCCCAC AGGACCTCCA CAGCCTGGCC 
GGGCTCAAGA TGGTCGAAGG TCAAGCAATT ATTCCAAAGT GCATTTCAGC TGGGATCATA 
TCCCAGGTCT TCCCTCTTCA CGAATTGCCT GCGTTGGAGA AACTGCAACG GACATGGGTT 
CGTGCGTTTC TCAGCCCCCA ACCCCTGGAC GACATTTGTA AATACTTCGG AGTCAAGATT 
ACCATGTATT TCGCGTGGCT CGGTCATTAC ACCACCGCTT TGATAGTTCC AGCTGTAGTG 
GGTGCCATAT ATTGGATCGG CATCATCGGT AGGAATCAAG CAGTGGAGGA CGTGGCGTAC 
GTACTGTTCT CTGTGTTCAA CGTAATCTGG GCCACCGTTT ACCTGGAAAC TTGGAAAAGA 
AGGGGTGCCG AACTGGCCTA CAGGTGGGGC ACGTTGGATC AAAGGGACGA CCTACTTCTC 
GAACCTAGGC CCTTATTCAC GGGTACCTTG GAAATTTCAT CGGTGACTGG AAGACTGGAG 
CCGACGTACC CTAGATGGAA GAGGAACATG TTCCGGTATT TCGTCAGCGT GCCCATCATT 
GCAGCGTGCC TGTTCTTCGT TTTCATCGTG ATGATTCTCA GCTTTCAGAT ACAGGATTGG 
TGGGACGCAC GCCTCGAGTC TAGGGGATAC GGTTTCTGGT TGAGCTACGT GCCAAAAGTC 
TTACTCGCGG TGGTGATAGC TTTAATGGAC GAGGCTTACT TCAAGGTTGC CGTATGGTTG 
AACGACATGG AAAACTATCG GCTAGACACC GAGTACGAGA ATCATCTGAT CTACAAGGTG 
GCACTGTTTC AGTTTGTAAA CTCCTTCCTA TCGCTATTTT ACATCGCCTT CTACCTACAG 
GATCAGGAGA GGCTGAAAGA GCAATTGGCC GCTCTGCTGA TAGTCCGGCA GGTGATCGGG 
CATCTGAAAG AGTCTGCAGT GCCGTATCTG ATCGAACAGC TACGACTCGC AAGGTTGAGC 
TTCGAGCTTT TCGGCGCGCT GAGCCCCAGT GAGGCTCGTC CTCCTCCTGG CGAGGACACC 
GAGGAAAATG GGAAGCAGGA GGGCGGCGAT GAGAAGAACG ATCGACCGGA CAGTGCGAAA 
GCGAAGCAGA GAAACGTCAG CCAGGCCGAG CTGGAGAGTT CCCTCTACAG AGTAGGGCAT 
CCCACTAATT CTCTACTTAG TGACGTTACG TTCAAGTACG ACGGGGCGTT TTCAGAGCAT 
CTGGAAATGC TGTCGCAACT GGGATACGTC TGCCTGTTCT CCTCGGCTTT CCCTCTGGCA 
GCGATGTTAG CGTTGCTTGG AAATCTTTTT GAGCTACGAG GCGACGCTTT CAAGCTGTGC 
TTTGTACTTC AGCGACCGTT CGGCCGTCGG GTCTCCAGCA TTGGAACTTG GCAGAACGCC 
ATGGAGGCGA TGGGTCTGGT CGCTATTTTG GTGAACTGCG CGCTGATCGG TCTCAGCGGG 
CAGGTTCAAC GGATGTTCCC GGAGATGTCG GCCACTCAGA CGATTCTGTT GATAGTTGCC 
CTTGAACATA TCATGCTGGC GATACGATTC ATCATAATCT GCGCGATTCC CGACATACCG 
CATTGGGTGG CCACGGAAAT GGCCAAGGTA GAGTTCCTGA GGAGAGAGGC GGTCAGAAGG 
TTATCCTCAA CGCCATCACC GGAGCAGCAA CCGGCTACAG TGATAGCAGA CGGTGAGAGC 
GAAGAGCAAC AATTGTTGTC AGAGGGCAAC GGCGACGCGA CGCCCTCCAG CATCCCGGAC 
ACGCCGACGC TGGCGTCGAC CACCCAGACG CCGAGCAGCC CTCCAACTCC TAGTGGTGCA 
TCCGTGCCGA GGATCTCGGA GACACCCTCC GCGGCTCAAA CTCCGGTCAG CTGTGCCAGC 
AGCGACTTGG GTAGTCCTTT ACTGACCGAC AAGAGCATCA TAGGCAGCGT CCGAGACATT 
CACCACGCGC CTAGGTGTTC CATTCCCGGT GGAGAACGAG GAAGGCGTTC AAGGGACTGG 
CTACAGACTG AATCGGAAGC GTCGGGTGGT GGCGATCAGT ACAGCCACCA TCTGACGATC 
GGTCCTCACG GTGGTATAGA CTGGGTCCGA AGATTAGGTC TCGAGACTGC TGTTCGAAAA 
TCCAGCGACT CCGAGATCAG CGGAGCTGGT ACCGTCAGTG GAAGTGGAGA CGAGTTGACT 
CTTCACAGGT CTACTGACTG CATCGTCTCG AAAGAACTCG CATCCTCATC GGATAGCGAT 
CTCCTCAGAT CCGCCCCGCC ATGGTCAGTG GCTCACAGGA ATCAGAAGTT CCGTTTCTCG 
CCCGAACGAG AGAAGGAACG GGAGAAGACC GAGAAGCAGC AGTATCAACA GTATCAGCGT 
GAAATTCAAG ATCACAAGCA ACAAGCAGCG TTGTACGCTG AAAAAGAGAA GGAGAAAGAG 
TCAAGTGCCC TCTCAGACTC GAGCAAAACT TCCAGTCAGG AGGAGGAGAA GCCGTCGAAG 
GAGGATCGAG AGGCGAAGAA GACCAGGGTG AAGCAGAGTT TAATGAAAAG AGCCAGGTCC 
GTGGCGATCT TCTCGTTAAA GCTGAAGGAG CGGCGCGCGA GGGAGGCCGA GATGAAGGCG 
AAGGAGGCGG AGAGGGAAGC CAGGTGGCAA CAGCCGCAGT CCTGCGTCGG CGGCGAACTC 
TCTTGCATTC CCATAGAAAA GCTTATATCT GTGGACGACA TCGCAGCCAT GGAGTTACGC 
AGACTTAATC ATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC108628482 ( XM_018030437.1 ) cDNA ORF clone, Ceratina calcarata -> XP_017885926.1 Ceratina calcarata anoctamin-8-like (LOC108628482), transcript variant X3, mRNA.

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CloneID	OCe149341
Clone ID Related Accession (Same CDS sequence)	XM_018030437.1 , XM_018030437.2
Accession Version	XM_018030437.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3372bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-08-30
Organism	Ceratina calcarata
Product	anoctamin-8-like isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017176396.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ceratina calcarata Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAGGCG GCGAGGGGTC CCCGATCAAT CTCCTCGACC AGCATCGTGC ATCGACCACC 
TCCGGCGAGT GCGCGAGTAC AAGTGACGAT GATGAGAAGA CTGCCACGGG CACCGTTTCC 
AGCACGATGA CGACCGTCTT CGCGGGCGAC GGTCTCAGAC GGAGGAAGGT CATACACGCG 
GCCAAAGAAA CCCTCGACAA GGCTTCGCGT CTGCTCAGAC GGAAGATACC ATGCACAGGC 
CACTTGATGA CCCCCCGCCG CCTATGGTTA CAAAAGGTTC CAACGCAGGA ATGCGACGTC 
GTGATGATGT TCCCGAGCGG AGCGAGCGAC GAGACCCTGA TGTGGCTTCT AGGCCGACTT 
CGGGCTGGAA CCCCGGGCTT GGTGGTGCAC GTGCGACACC ATGCCTCTTC AGATAGTTAC 
AGCTTTTACT TAACTGCGCC GTTTAGCGTA CTTTTGAAAG CAGCTGAGGA GGTGCATTTA 
CCGAAGACGC TGCGACAGGA GTTCGGCGGT GGACTGAAGG AGTTCGTTGG CTCCGAGGCG 
AGCTGCTTCG AGGGTAGTGA CGATGAAGCT CAGTTCTTCA CCACCCAGGA GAGGCAATCG 
CTGGTACTCC ACCTGCTGCA CACTCTCAGG GCGGGCCCAC AGGACCTCCA CAGCCTGGCC 
GGGCTCAAGA TGGTCGAAGG TCAAGCAATT ATTCCAAAGT GCATTTCAGC TGGGATCATA 
TCCCAGGTCT TCCCTCTTCA CGAATTGCCT GCGTTGGAGA AACTGCAACG GACATGGGTT 
CGTGCGTTTC TCAGCCCCCA ACCCCTGGAC GACATTTGTA AATACTTCGG AGTCAAGATT 
ACCATGTATT TCGCGTGGCT CGGTCATTAC ACCACCGCTT TGATAGTTCC AGCTGTAGTG 
GGTGCCATAT ATTGGATCGG CATCATCGGT AGGAATCAAG CAGTGGAGGA CGTGGCGTAC 
GTACTGTTCT CTGTGTTCAA CGTAATCTGG GCCACCGTTT ACCTGGAAAC TTGGAAAAGA 
AGGGGTGCCG AACTGGCCTA CAGGTGGGGC ACGTTGGATC AAAGGGACGA CCTACTTCTC 
GAACCTAGGC CCTTATTCAC GGGTACCTTG GAAATTTCAT CGGTGACTGG AAGACTGGAG 
CCGACGTACC CTAGATGGAA GAGGAACATG TTCCGGTATT TCGTCAGCGT GCCCATCATT 
GCAGCGTGCC TGTTCTTCGT TTTCATCGTG ATGATTCTCA GCTTTCAGAT ACAGGATTGG 
TGGGACGCAC GCCTCGAGTC TAGGGGATAC GGTTTCTGGT TGAGCTACGT GCCAAAAGTC 
TTACTCGCGG TGGTGATAGC TTTAATGGAC GAGGCTTACT TCAAGGTTGC CGTATGGTTG 
AACGACATGG AAAACTATCG GCTAGACACC GAGTACGAGA ATCATCTGAT CTACAAGGTG 
GCACTGTTTC AGTTTGTAAA CTCCTTCCTA TCGCTATTTT ACATCGCCTT CTACCTACAG 
GATCAGGAGA GGCTGAAAGA GCAATTGGCC GCTCTGCTGA TAGTCCGGCA GGTGATCGGG 
CATCTGAAAG AGTCTGCAGT GCCGTATCTG ATCGAACAGC TACGACTCGC AAGGTTGAGC 
TTCGAGCTTT TCGGCGCGCT GAGCCCCAGT GAGGCTCGTC CTCCTCCTGG CGAGGACACC 
GAGGAAAATG GGAAGCAGGA GGGCGGCGAT GAGAAGAACG ATCGACCGGA CAGTGCGAAA 
GCGAAGCAGA GAAACGTCAG CCAGGCCGAG CTGGAGAGTT CCCTCTACAG AGTAGGGCAT 
CCCACTAATT CTCTACTTAG TGACGTTACG TTCAAGTACG ACGGGGCGTT TTCAGAGCAT 
CTGGAAATGC TGTCGCAACT GGGATACGTC TGCCTGTTCT CCTCGGCTTT CCCTCTGGCA 
GCGATGTTAG CGTTGCTTGG AAATCTTTTT GAGCTACGAG GCGACGCTTT CAAGCTGTGC 
TTTGTACTTC AGCGACCGTT CGGCCGTCGG GTCTCCAGCA TTGGAACTTG GCAGAACGCC 
ATGGAGGCGA TGGGTCTGGT CGCTATTTTG GTGAACTGCG CGCTGATCGG TCTCAGCGGG 
CAGGTTCAAC GGATGTTCCC GGAGATGTCG GCCACTCAGA CGATTCTGTT GATAGTTGCC 
CTTGAACATA TCATGCTGGC GATACGATTC ATCATAATCT GCGCGATTCC CGACATACCG 
CATTGGGTGG CCACGGAAAT GGCCAAGGTA GAGTTCCTGA GGAGAGAGGC GGTCAGAAGG 
TTATCCTCAA CGCCATCACC GGAGCAGCAA CCGGCTACAG TGATAGACGG TGAGAGCGAA 
GAGCAACAAT TGTTGTCAGA GGGCAACGGC GACGCGACGC CCTCCAGCAT CCCGGACACG 
CCGACGCTGG CGTCGACCAC CCAGACGCCG AGCAGCCCTC CAACTCCTAG TGGTGCATCC 
GTGCCGAGGA TCTCGGAGAC ACCCTCCGCG GCTCAAACTC CGGTCAGCTG TGCCAGCAGC 
GACTTGGGTA GTCCTTTACT GACCGACAAG AGCATCATAG GCAGCGTCCG AGACATTCAC 
CACGCGCCTA GGTGTTCCAT TCCCGGTGGA GAACGAGGAA GGCGTTCAAG GGACTGGCTA 
CAGACTGAAT CGGAAGCGTC GGGTGGTGGC GATCAGTACA GCCACCATCT GACGATCGGT 
CCTCACGGTG GTATAGACTG GGTCCGAAGA TTAGGTCTCG AGACTGCTGT TCGAAAATCC 
AGCGACTCCG AGATCAGCGG AGCTGGTACC GTCAGTGGAA GTGGAGACGA GTTGACTCTT 
CACAGGTCTA CTGACTGCAT CGTCTCGAAA GAACTCGCAT CCTCATCGGA TAGCGATCTC 
CTCAGATCCG CCCCGCCATG GTCAGTGGCT CACAGGAATC AGAAGTTCCG TTTCTCGCCC 
GAACGAGAGA AGGAACGGGA GAAGACCGAG AAGCAGCAGT ATCAACAGTA TCAGCGTGAA 
ATTCAAGATC ACAAGCAACA AGCAGCGTTG TACGCTGAAA AAGAGAAGGA GAAAGAGTCA 
AGTGCCCTCT CAGACTCGAG CAAAACTTCC AGTCAGGAGG AGGAGAAGCC GTCGAAGGAG 
GATCGAGAGG CGAAGAAGAC CAGGGTGAAG CAGAGTTTAA TGAAAAGAGC CAGGTCCGTG 
GCGATCTTCT CGTTAAAGCT GAAGGAGCGG CGCGCGAGGG AGGCCGAGAT GAAGGCGAAG 
GAGGCGGAGA GGGAAGCCAG GTGGCAACAG CCGCAGTCCT GCGTCGGCGG CGAACTCTCT 
TGCATTCCCA TAGAAAAGCT TATATCTGTG GACGACATCG CAGCCATGGA GTTACGCAGA 
CTTAATCATT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017885926.1
CDS	64..3435
Translation	MPGGEGSPINLLDQHRASTTSGECASTSDDDEKTATGTVSSTMTTVFAGDGLRRRKVIHAAKETLDKASRLLRRKIPCTGHLMTPRRLWLQKVPTQECDVVMMFPSGASDETLMWLLGRLRAGTPGLVVHVRHHASSDSYSFYLTAPFSVLLKAAEEVHLPKTLRQEFGGGLKEFVGSEASCFEGSDDEAQFFTTQERQSLVLHLLHTLRAGPQDLHSLAGLKMVEGQAIIPKCISAGIISQVFPLHELPALEKLQRTWVRAFLSPQPLDDICKYFGVKITMYFAWLGHYTTALIVPAVVGAIYWIGIIGRNQAVEDVAYVLFSVFNVIWATVYLETWKRRGAELAYRWGTLDQRDDLLLEPRPLFTGTLEISSVTGRLEPTYPRWKRNMFRYFVSVPIIAACLFFVFIVMILSFQIQDWWDARLESRGYGFWLSYVPKVLLAVVIALMDEAYFKVAVWLNDMENYRLDTEYENHLIYKVALFQFVNSFLSLFYIAFYLQDQERLKEQLAALLIVRQVIGHLKESAVPYLIEQLRLARLSFELFGALSPSEARPPPGEDTEENGKQEGGDEKNDRPDSAKAKQRNVSQAELESSLYRVGHPTNSLLSDVTFKYDGAFSEHLEMLSQLGYVCLFSSAFPLAAMLALLGNLFELRGDAFKLCFVLQRPFGRRVSSIGTWQNAMEAMGLVAILVNCALIGLSGQVQRMFPEMSATQTILLIVALEHIMLAIRFIIICAIPDIPHWVATEMAKVEFLRREAVRRLSSTPSPEQQPATVIDGESEEQQLLSEGNGDATPSSIPDTPTLASTTQTPSSPPTPSGASVPRISETPSAAQTPVSCASSDLGSPLLTDKSIIGSVRDIHHAPRCSIPGGERGRRSRDWLQTESEASGGGDQYSHHLTIGPHGGIDWVRRLGLETAVRKSSDSEISGAGTVSGSGDELTLHRSTDCIVSKELASSSDSDLLRSAPPWSVAHRNQKFRFSPEREKEREKTEKQQYQQYQREIQDHKQQAALYAEKEKEKESSALSDSSKTSSQEEEKPSKEDREAKKTRVKQSLMKRARSVAIFSLKLKERRAREAEMKAKEAEREARWQQPQSCVGGELSCIPIEKLISVDDIAAMELRRLNH

Target ORF information:

RefSeq Version	XM_018030437.1
Organism	Ceratina calcarata
Definition	Ceratina calcarata anoctamin-8-like (LOC108628482), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018030437.1

ORF Insert Sequence:

ATGCCAGGCG GCGAGGGGTC CCCGATCAAT CTCCTCGACC AGCATCGTGC ATCGACCACC 
TCCGGCGAGT GCGCGAGTAC AAGTGACGAT GATGAGAAGA CTGCCACGGG CACCGTTTCC 
AGCACGATGA CGACCGTCTT CGCGGGCGAC GGTCTCAGAC GGAGGAAGGT CATACACGCG 
GCCAAAGAAA CCCTCGACAA GGCTTCGCGT CTGCTCAGAC GGAAGATACC ATGCACAGGC 
CACTTGATGA CCCCCCGCCG CCTATGGTTA CAAAAGGTTC CAACGCAGGA ATGCGACGTC 
GTGATGATGT TCCCGAGCGG AGCGAGCGAC GAGACCCTGA TGTGGCTTCT AGGCCGACTT 
CGGGCTGGAA CCCCGGGCTT GGTGGTGCAC GTGCGACACC ATGCCTCTTC AGATAGTTAC 
AGCTTTTACT TAACTGCGCC GTTTAGCGTA CTTTTGAAAG CAGCTGAGGA GGTGCATTTA 
CCGAAGACGC TGCGACAGGA GTTCGGCGGT GGACTGAAGG AGTTCGTTGG CTCCGAGGCG 
AGCTGCTTCG AGGGTAGTGA CGATGAAGCT CAGTTCTTCA CCACCCAGGA GAGGCAATCG 
CTGGTACTCC ACCTGCTGCA CACTCTCAGG GCGGGCCCAC AGGACCTCCA CAGCCTGGCC 
GGGCTCAAGA TGGTCGAAGG TCAAGCAATT ATTCCAAAGT GCATTTCAGC TGGGATCATA 
TCCCAGGTCT TCCCTCTTCA CGAATTGCCT GCGTTGGAGA AACTGCAACG GACATGGGTT 
CGTGCGTTTC TCAGCCCCCA ACCCCTGGAC GACATTTGTA AATACTTCGG AGTCAAGATT 
ACCATGTATT TCGCGTGGCT CGGTCATTAC ACCACCGCTT TGATAGTTCC AGCTGTAGTG 
GGTGCCATAT ATTGGATCGG CATCATCGGT AGGAATCAAG CAGTGGAGGA CGTGGCGTAC 
GTACTGTTCT CTGTGTTCAA CGTAATCTGG GCCACCGTTT ACCTGGAAAC TTGGAAAAGA 
AGGGGTGCCG AACTGGCCTA CAGGTGGGGC ACGTTGGATC AAAGGGACGA CCTACTTCTC 
GAACCTAGGC CCTTATTCAC GGGTACCTTG GAAATTTCAT CGGTGACTGG AAGACTGGAG 
CCGACGTACC CTAGATGGAA GAGGAACATG TTCCGGTATT TCGTCAGCGT GCCCATCATT 
GCAGCGTGCC TGTTCTTCGT TTTCATCGTG ATGATTCTCA GCTTTCAGAT ACAGGATTGG 
TGGGACGCAC GCCTCGAGTC TAGGGGATAC GGTTTCTGGT TGAGCTACGT GCCAAAAGTC 
TTACTCGCGG TGGTGATAGC TTTAATGGAC GAGGCTTACT TCAAGGTTGC CGTATGGTTG 
AACGACATGG AAAACTATCG GCTAGACACC GAGTACGAGA ATCATCTGAT CTACAAGGTG 
GCACTGTTTC AGTTTGTAAA CTCCTTCCTA TCGCTATTTT ACATCGCCTT CTACCTACAG 
GATCAGGAGA GGCTGAAAGA GCAATTGGCC GCTCTGCTGA TAGTCCGGCA GGTGATCGGG 
CATCTGAAAG AGTCTGCAGT GCCGTATCTG ATCGAACAGC TACGACTCGC AAGGTTGAGC 
TTCGAGCTTT TCGGCGCGCT GAGCCCCAGT GAGGCTCGTC CTCCTCCTGG CGAGGACACC 
GAGGAAAATG GGAAGCAGGA GGGCGGCGAT GAGAAGAACG ATCGACCGGA CAGTGCGAAA 
GCGAAGCAGA GAAACGTCAG CCAGGCCGAG CTGGAGAGTT CCCTCTACAG AGTAGGGCAT 
CCCACTAATT CTCTACTTAG TGACGTTACG TTCAAGTACG ACGGGGCGTT TTCAGAGCAT 
CTGGAAATGC TGTCGCAACT GGGATACGTC TGCCTGTTCT CCTCGGCTTT CCCTCTGGCA 
GCGATGTTAG CGTTGCTTGG AAATCTTTTT GAGCTACGAG GCGACGCTTT CAAGCTGTGC 
TTTGTACTTC AGCGACCGTT CGGCCGTCGG GTCTCCAGCA TTGGAACTTG GCAGAACGCC 
ATGGAGGCGA TGGGTCTGGT CGCTATTTTG GTGAACTGCG CGCTGATCGG TCTCAGCGGG 
CAGGTTCAAC GGATGTTCCC GGAGATGTCG GCCACTCAGA CGATTCTGTT GATAGTTGCC 
CTTGAACATA TCATGCTGGC GATACGATTC ATCATAATCT GCGCGATTCC CGACATACCG 
CATTGGGTGG CCACGGAAAT GGCCAAGGTA GAGTTCCTGA GGAGAGAGGC GGTCAGAAGG 
TTATCCTCAA CGCCATCACC GGAGCAGCAA CCGGCTACAG TGATAGACGG TGAGAGCGAA 
GAGCAACAAT TGTTGTCAGA GGGCAACGGC GACGCGACGC CCTCCAGCAT CCCGGACACG 
CCGACGCTGG CGTCGACCAC CCAGACGCCG AGCAGCCCTC CAACTCCTAG TGGTGCATCC 
GTGCCGAGGA TCTCGGAGAC ACCCTCCGCG GCTCAAACTC CGGTCAGCTG TGCCAGCAGC 
GACTTGGGTA GTCCTTTACT GACCGACAAG AGCATCATAG GCAGCGTCCG AGACATTCAC 
CACGCGCCTA GGTGTTCCAT TCCCGGTGGA GAACGAGGAA GGCGTTCAAG GGACTGGCTA 
CAGACTGAAT CGGAAGCGTC GGGTGGTGGC GATCAGTACA GCCACCATCT GACGATCGGT 
CCTCACGGTG GTATAGACTG GGTCCGAAGA TTAGGTCTCG AGACTGCTGT TCGAAAATCC 
AGCGACTCCG AGATCAGCGG AGCTGGTACC GTCAGTGGAA GTGGAGACGA GTTGACTCTT 
CACAGGTCTA CTGACTGCAT CGTCTCGAAA GAACTCGCAT CCTCATCGGA TAGCGATCTC 
CTCAGATCCG CCCCGCCATG GTCAGTGGCT CACAGGAATC AGAAGTTCCG TTTCTCGCCC 
GAACGAGAGA AGGAACGGGA GAAGACCGAG AAGCAGCAGT ATCAACAGTA TCAGCGTGAA 
ATTCAAGATC ACAAGCAACA AGCAGCGTTG TACGCTGAAA AAGAGAAGGA GAAAGAGTCA 
AGTGCCCTCT CAGACTCGAG CAAAACTTCC AGTCAGGAGG AGGAGAAGCC GTCGAAGGAG 
GATCGAGAGG CGAAGAAGAC CAGGGTGAAG CAGAGTTTAA TGAAAAGAGC CAGGTCCGTG 
GCGATCTTCT CGTTAAAGCT GAAGGAGCGG CGCGCGAGGG AGGCCGAGAT GAAGGCGAAG 
GAGGCGGAGA GGGAAGCCAG GTGGCAACAG CCGCAGTCCT GCGTCGGCGG CGAACTCTCT 
TGCATTCCCA TAGAAAAGCT TATATCTGTG GACGACATCG CAGCCATGGA GTTACGCAGA 
CTTAATCATT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC108628482 ( XM_018030437.2 ) cDNA ORF clone, Ceratina calcarata -> XP_017885926.1 Ceratina calcarata anoctamin-8-like (LOC108628482), transcript variant X3, mRNA.

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CloneID	OCe149341
Clone ID Related Accession (Same CDS sequence)	XM_018030437.1 , XM_018030437.2
Accession Version	XM_018030437.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3372bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-10-16
Organism	Ceratina calcarata
Product	anoctamin-8-like isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017176396.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Oct 17, 2018 this sequence version replaced XM_018030437.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ceratina calcarata Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAGGCG GCGAGGGGTC CCCGATCAAT CTCCTCGACC AGCATCGTGC ATCGACCACC 
TCCGGCGAGT GCGCGAGTAC AAGTGACGAT GATGAGAAGA CTGCCACGGG CACCGTTTCC 
AGCACGATGA CGACCGTCTT CGCGGGCGAC GGTCTCAGAC GGAGGAAGGT CATACACGCG 
GCCAAAGAAA CCCTCGACAA GGCTTCGCGT CTGCTCAGAC GGAAGATACC ATGCACAGGC 
CACTTGATGA CCCCCCGCCG CCTATGGTTA CAAAAGGTTC CAACGCAGGA ATGCGACGTC 
GTGATGATGT TCCCGAGCGG AGCGAGCGAC GAGACCCTGA TGTGGCTTCT AGGCCGACTT 
CGGGCTGGAA CCCCGGGCTT GGTGGTGCAC GTGCGACACC ATGCCTCTTC AGATAGTTAC 
AGCTTTTACT TAACTGCGCC GTTTAGCGTA CTTTTGAAAG CAGCTGAGGA GGTGCATTTA 
CCGAAGACGC TGCGACAGGA GTTCGGCGGT GGACTGAAGG AGTTCGTTGG CTCCGAGGCG 
AGCTGCTTCG AGGGTAGTGA CGATGAAGCT CAGTTCTTCA CCACCCAGGA GAGGCAATCG 
CTGGTACTCC ACCTGCTGCA CACTCTCAGG GCGGGCCCAC AGGACCTCCA CAGCCTGGCC 
GGGCTCAAGA TGGTCGAAGG TCAAGCAATT ATTCCAAAGT GCATTTCAGC TGGGATCATA 
TCCCAGGTCT TCCCTCTTCA CGAATTGCCT GCGTTGGAGA AACTGCAACG GACATGGGTT 
CGTGCGTTTC TCAGCCCCCA ACCCCTGGAC GACATTTGTA AATACTTCGG AGTCAAGATT 
ACCATGTATT TCGCGTGGCT CGGTCATTAC ACCACCGCTT TGATAGTTCC AGCTGTAGTG 
GGTGCCATAT ATTGGATCGG CATCATCGGT AGGAATCAAG CAGTGGAGGA CGTGGCGTAC 
GTACTGTTCT CTGTGTTCAA CGTAATCTGG GCCACCGTTT ACCTGGAAAC TTGGAAAAGA 
AGGGGTGCCG AACTGGCCTA CAGGTGGGGC ACGTTGGATC AAAGGGACGA CCTACTTCTC 
GAACCTAGGC CCTTATTCAC GGGTACCTTG GAAATTTCAT CGGTGACTGG AAGACTGGAG 
CCGACGTACC CTAGATGGAA GAGGAACATG TTCCGGTATT TCGTCAGCGT GCCCATCATT 
GCAGCGTGCC TGTTCTTCGT TTTCATCGTG ATGATTCTCA GCTTTCAGAT ACAGGATTGG 
TGGGACGCAC GCCTCGAGTC TAGGGGATAC GGTTTCTGGT TGAGCTACGT GCCAAAAGTC 
TTACTCGCGG TGGTGATAGC TTTAATGGAC GAGGCTTACT TCAAGGTTGC CGTATGGTTG 
AACGACATGG AAAACTATCG GCTAGACACC GAGTACGAGA ATCATCTGAT CTACAAGGTG 
GCACTGTTTC AGTTTGTAAA CTCCTTCCTA TCGCTATTTT ACATCGCCTT CTACCTACAG 
GATCAGGAGA GGCTGAAAGA GCAATTGGCC GCTCTGCTGA TAGTCCGGCA GGTGATCGGG 
CATCTGAAAG AGTCTGCAGT GCCGTATCTG ATCGAACAGC TACGACTCGC AAGGTTGAGC 
TTCGAGCTTT TCGGCGCGCT GAGCCCCAGT GAGGCTCGTC CTCCTCCTGG CGAGGACACC 
GAGGAAAATG GGAAGCAGGA GGGCGGCGAT GAGAAGAACG ATCGACCGGA CAGTGCGAAA 
GCGAAGCAGA GAAACGTCAG CCAGGCCGAG CTGGAGAGTT CCCTCTACAG AGTAGGGCAT 
CCCACTAATT CTCTACTTAG TGACGTTACG TTCAAGTACG ACGGGGCGTT TTCAGAGCAT 
CTGGAAATGC TGTCGCAACT GGGATACGTC TGCCTGTTCT CCTCGGCTTT CCCTCTGGCA 
GCGATGTTAG CGTTGCTTGG AAATCTTTTT GAGCTACGAG GCGACGCTTT CAAGCTGTGC 
TTTGTACTTC AGCGACCGTT CGGCCGTCGG GTCTCCAGCA TTGGAACTTG GCAGAACGCC 
ATGGAGGCGA TGGGTCTGGT CGCTATTTTG GTGAACTGCG CGCTGATCGG TCTCAGCGGG 
CAGGTTCAAC GGATGTTCCC GGAGATGTCG GCCACTCAGA CGATTCTGTT GATAGTTGCC 
CTTGAACATA TCATGCTGGC GATACGATTC ATCATAATCT GCGCGATTCC CGACATACCG 
CATTGGGTGG CCACGGAAAT GGCCAAGGTA GAGTTCCTGA GGAGAGAGGC GGTCAGAAGG 
TTATCCTCAA CGCCATCACC GGAGCAGCAA CCGGCTACAG TGATAGACGG TGAGAGCGAA 
GAGCAACAAT TGTTGTCAGA GGGCAACGGC GACGCGACGC CCTCCAGCAT CCCGGACACG 
CCGACGCTGG CGTCGACCAC CCAGACGCCG AGCAGCCCTC CAACTCCTAG TGGTGCATCC 
GTGCCGAGGA TCTCGGAGAC ACCCTCCGCG GCTCAAACTC CGGTCAGCTG TGCCAGCAGC 
GACTTGGGTA GTCCTTTACT GACCGACAAG AGCATCATAG GCAGCGTCCG AGACATTCAC 
CACGCGCCTA GGTGTTCCAT TCCCGGTGGA GAACGAGGAA GGCGTTCAAG GGACTGGCTA 
CAGACTGAAT CGGAAGCGTC GGGTGGTGGC GATCAGTACA GCCACCATCT GACGATCGGT 
CCTCACGGTG GTATAGACTG GGTCCGAAGA TTAGGTCTCG AGACTGCTGT TCGAAAATCC 
AGCGACTCCG AGATCAGCGG AGCTGGTACC GTCAGTGGAA GTGGAGACGA GTTGACTCTT 
CACAGGTCTA CTGACTGCAT CGTCTCGAAA GAACTCGCAT CCTCATCGGA TAGCGATCTC 
CTCAGATCCG CCCCGCCATG GTCAGTGGCT CACAGGAATC AGAAGTTCCG TTTCTCGCCC 
GAACGAGAGA AGGAACGGGA GAAGACCGAG AAGCAGCAGT ATCAACAGTA TCAGCGTGAA 
ATTCAAGATC ACAAGCAACA AGCAGCGTTG TACGCTGAAA AAGAGAAGGA GAAAGAGTCA 
AGTGCCCTCT CAGACTCGAG CAAAACTTCC AGTCAGGAGG AGGAGAAGCC GTCGAAGGAG 
GATCGAGAGG CGAAGAAGAC CAGGGTGAAG CAGAGTTTAA TGAAAAGAGC CAGGTCCGTG 
GCGATCTTCT CGTTAAAGCT GAAGGAGCGG CGCGCGAGGG AGGCCGAGAT GAAGGCGAAG 
GAGGCGGAGA GGGAAGCCAG GTGGCAACAG CCGCAGTCCT GCGTCGGCGG CGAACTCTCT 
TGCATTCCCA TAGAAAAGCT TATATCTGTG GACGACATCG CAGCCATGGA GTTACGCAGA 
CTTAATCATT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017885926.1
CDS	96..3467
Translation	MPGGEGSPINLLDQHRASTTSGECASTSDDDEKTATGTVSSTMTTVFAGDGLRRRKVIHAAKETLDKASRLLRRKIPCTGHLMTPRRLWLQKVPTQECDVVMMFPSGASDETLMWLLGRLRAGTPGLVVHVRHHASSDSYSFYLTAPFSVLLKAAEEVHLPKTLRQEFGGGLKEFVGSEASCFEGSDDEAQFFTTQERQSLVLHLLHTLRAGPQDLHSLAGLKMVEGQAIIPKCISAGIISQVFPLHELPALEKLQRTWVRAFLSPQPLDDICKYFGVKITMYFAWLGHYTTALIVPAVVGAIYWIGIIGRNQAVEDVAYVLFSVFNVIWATVYLETWKRRGAELAYRWGTLDQRDDLLLEPRPLFTGTLEISSVTGRLEPTYPRWKRNMFRYFVSVPIIAACLFFVFIVMILSFQIQDWWDARLESRGYGFWLSYVPKVLLAVVIALMDEAYFKVAVWLNDMENYRLDTEYENHLIYKVALFQFVNSFLSLFYIAFYLQDQERLKEQLAALLIVRQVIGHLKESAVPYLIEQLRLARLSFELFGALSPSEARPPPGEDTEENGKQEGGDEKNDRPDSAKAKQRNVSQAELESSLYRVGHPTNSLLSDVTFKYDGAFSEHLEMLSQLGYVCLFSSAFPLAAMLALLGNLFELRGDAFKLCFVLQRPFGRRVSSIGTWQNAMEAMGLVAILVNCALIGLSGQVQRMFPEMSATQTILLIVALEHIMLAIRFIIICAIPDIPHWVATEMAKVEFLRREAVRRLSSTPSPEQQPATVIDGESEEQQLLSEGNGDATPSSIPDTPTLASTTQTPSSPPTPSGASVPRISETPSAAQTPVSCASSDLGSPLLTDKSIIGSVRDIHHAPRCSIPGGERGRRSRDWLQTESEASGGGDQYSHHLTIGPHGGIDWVRRLGLETAVRKSSDSEISGAGTVSGSGDELTLHRSTDCIVSKELASSSDSDLLRSAPPWSVAHRNQKFRFSPEREKEREKTEKQQYQQYQREIQDHKQQAALYAEKEKEKESSALSDSSKTSSQEEEKPSKEDREAKKTRVKQSLMKRARSVAIFSLKLKERRAREAEMKAKEAEREARWQQPQSCVGGELSCIPIEKLISVDDIAAMELRRLNH

Target ORF information:

RefSeq Version	XM_018030437.2
Organism	Ceratina calcarata
Definition	Ceratina calcarata anoctamin-8-like (LOC108628482), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018030437.2

ORF Insert Sequence:

ATGCCAGGCG GCGAGGGGTC CCCGATCAAT CTCCTCGACC AGCATCGTGC ATCGACCACC 
TCCGGCGAGT GCGCGAGTAC AAGTGACGAT GATGAGAAGA CTGCCACGGG CACCGTTTCC 
AGCACGATGA CGACCGTCTT CGCGGGCGAC GGTCTCAGAC GGAGGAAGGT CATACACGCG 
GCCAAAGAAA CCCTCGACAA GGCTTCGCGT CTGCTCAGAC GGAAGATACC ATGCACAGGC 
CACTTGATGA CCCCCCGCCG CCTATGGTTA CAAAAGGTTC CAACGCAGGA ATGCGACGTC 
GTGATGATGT TCCCGAGCGG AGCGAGCGAC GAGACCCTGA TGTGGCTTCT AGGCCGACTT 
CGGGCTGGAA CCCCGGGCTT GGTGGTGCAC GTGCGACACC ATGCCTCTTC AGATAGTTAC 
AGCTTTTACT TAACTGCGCC GTTTAGCGTA CTTTTGAAAG CAGCTGAGGA GGTGCATTTA 
CCGAAGACGC TGCGACAGGA GTTCGGCGGT GGACTGAAGG AGTTCGTTGG CTCCGAGGCG 
AGCTGCTTCG AGGGTAGTGA CGATGAAGCT CAGTTCTTCA CCACCCAGGA GAGGCAATCG 
CTGGTACTCC ACCTGCTGCA CACTCTCAGG GCGGGCCCAC AGGACCTCCA CAGCCTGGCC 
GGGCTCAAGA TGGTCGAAGG TCAAGCAATT ATTCCAAAGT GCATTTCAGC TGGGATCATA 
TCCCAGGTCT TCCCTCTTCA CGAATTGCCT GCGTTGGAGA AACTGCAACG GACATGGGTT 
CGTGCGTTTC TCAGCCCCCA ACCCCTGGAC GACATTTGTA AATACTTCGG AGTCAAGATT 
ACCATGTATT TCGCGTGGCT CGGTCATTAC ACCACCGCTT TGATAGTTCC AGCTGTAGTG 
GGTGCCATAT ATTGGATCGG CATCATCGGT AGGAATCAAG CAGTGGAGGA CGTGGCGTAC 
GTACTGTTCT CTGTGTTCAA CGTAATCTGG GCCACCGTTT ACCTGGAAAC TTGGAAAAGA 
AGGGGTGCCG AACTGGCCTA CAGGTGGGGC ACGTTGGATC AAAGGGACGA CCTACTTCTC 
GAACCTAGGC CCTTATTCAC GGGTACCTTG GAAATTTCAT CGGTGACTGG AAGACTGGAG 
CCGACGTACC CTAGATGGAA GAGGAACATG TTCCGGTATT TCGTCAGCGT GCCCATCATT 
GCAGCGTGCC TGTTCTTCGT TTTCATCGTG ATGATTCTCA GCTTTCAGAT ACAGGATTGG 
TGGGACGCAC GCCTCGAGTC TAGGGGATAC GGTTTCTGGT TGAGCTACGT GCCAAAAGTC 
TTACTCGCGG TGGTGATAGC TTTAATGGAC GAGGCTTACT TCAAGGTTGC CGTATGGTTG 
AACGACATGG AAAACTATCG GCTAGACACC GAGTACGAGA ATCATCTGAT CTACAAGGTG 
GCACTGTTTC AGTTTGTAAA CTCCTTCCTA TCGCTATTTT ACATCGCCTT CTACCTACAG 
GATCAGGAGA GGCTGAAAGA GCAATTGGCC GCTCTGCTGA TAGTCCGGCA GGTGATCGGG 
CATCTGAAAG AGTCTGCAGT GCCGTATCTG ATCGAACAGC TACGACTCGC AAGGTTGAGC 
TTCGAGCTTT TCGGCGCGCT GAGCCCCAGT GAGGCTCGTC CTCCTCCTGG CGAGGACACC 
GAGGAAAATG GGAAGCAGGA GGGCGGCGAT GAGAAGAACG ATCGACCGGA CAGTGCGAAA 
GCGAAGCAGA GAAACGTCAG CCAGGCCGAG CTGGAGAGTT CCCTCTACAG AGTAGGGCAT 
CCCACTAATT CTCTACTTAG TGACGTTACG TTCAAGTACG ACGGGGCGTT TTCAGAGCAT 
CTGGAAATGC TGTCGCAACT GGGATACGTC TGCCTGTTCT CCTCGGCTTT CCCTCTGGCA 
GCGATGTTAG CGTTGCTTGG AAATCTTTTT GAGCTACGAG GCGACGCTTT CAAGCTGTGC 
TTTGTACTTC AGCGACCGTT CGGCCGTCGG GTCTCCAGCA TTGGAACTTG GCAGAACGCC 
ATGGAGGCGA TGGGTCTGGT CGCTATTTTG GTGAACTGCG CGCTGATCGG TCTCAGCGGG 
CAGGTTCAAC GGATGTTCCC GGAGATGTCG GCCACTCAGA CGATTCTGTT GATAGTTGCC 
CTTGAACATA TCATGCTGGC GATACGATTC ATCATAATCT GCGCGATTCC CGACATACCG 
CATTGGGTGG CCACGGAAAT GGCCAAGGTA GAGTTCCTGA GGAGAGAGGC GGTCAGAAGG 
TTATCCTCAA CGCCATCACC GGAGCAGCAA CCGGCTACAG TGATAGACGG TGAGAGCGAA 
GAGCAACAAT TGTTGTCAGA GGGCAACGGC GACGCGACGC CCTCCAGCAT CCCGGACACG 
CCGACGCTGG CGTCGACCAC CCAGACGCCG AGCAGCCCTC CAACTCCTAG TGGTGCATCC 
GTGCCGAGGA TCTCGGAGAC ACCCTCCGCG GCTCAAACTC CGGTCAGCTG TGCCAGCAGC 
GACTTGGGTA GTCCTTTACT GACCGACAAG AGCATCATAG GCAGCGTCCG AGACATTCAC 
CACGCGCCTA GGTGTTCCAT TCCCGGTGGA GAACGAGGAA GGCGTTCAAG GGACTGGCTA 
CAGACTGAAT CGGAAGCGTC GGGTGGTGGC GATCAGTACA GCCACCATCT GACGATCGGT 
CCTCACGGTG GTATAGACTG GGTCCGAAGA TTAGGTCTCG AGACTGCTGT TCGAAAATCC 
AGCGACTCCG AGATCAGCGG AGCTGGTACC GTCAGTGGAA GTGGAGACGA GTTGACTCTT 
CACAGGTCTA CTGACTGCAT CGTCTCGAAA GAACTCGCAT CCTCATCGGA TAGCGATCTC 
CTCAGATCCG CCCCGCCATG GTCAGTGGCT CACAGGAATC AGAAGTTCCG TTTCTCGCCC 
GAACGAGAGA AGGAACGGGA GAAGACCGAG AAGCAGCAGT ATCAACAGTA TCAGCGTGAA 
ATTCAAGATC ACAAGCAACA AGCAGCGTTG TACGCTGAAA AAGAGAAGGA GAAAGAGTCA 
AGTGCCCTCT CAGACTCGAG CAAAACTTCC AGTCAGGAGG AGGAGAAGCC GTCGAAGGAG 
GATCGAGAGG CGAAGAAGAC CAGGGTGAAG CAGAGTTTAA TGAAAAGAGC CAGGTCCGTG 
GCGATCTTCT CGTTAAAGCT GAAGGAGCGG CGCGCGAGGG AGGCCGAGAT GAAGGCGAAG 
GAGGCGGAGA GGGAAGCCAG GTGGCAACAG CCGCAGTCCT GCGTCGGCGG CGAACTCTCT 
TGCATTCCCA TAGAAAAGCT TATATCTGTG GACGACATCG CAGCCATGGA GTTACGCAGA 
CTTAATCATT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC108628482 ( XM_018030438.2 ) cDNA ORF clone, Ceratina calcarata -> XP_017885927.1 Ceratina calcarata anoctamin-8-like (LOC108628482), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OCe149342
Clone ID Related Accession (Same CDS sequence)	XM_018030438.2 , XM_018030438.1
Accession Version	XM_018030438.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3351bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-10-16
Organism	Ceratina calcarata
Product	anoctamin-8-like isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017176396.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Oct 17, 2018 this sequence version replaced XM_018030438.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Ceratina calcarata Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAGGCG GCGAGGGGTC CCCGATCAAT CTCCTCGACC AGCATCGTGC ATCGACCACC 
TCCGGCGAGT GCGCGAGTAC AAGTGACGAT GATGAGAAGA CTGCCACGGG CACCGTTTCC 
AGCACGATGA CGACCGTCTT CGCGGGCGAC GGTCTCAGAC GGAGGAAGGT CATACACGCG 
GCCAAAGAAA CCCTCGACAA GGCTTCGCGT CTGCTCAGAC GGAAGATACC ATGCACAGGC 
CACTTGATGA CCCCCCGCCG CCTATGGTTA CAAAAGGTTC CAACGCAGGA ATGCGACGTC 
GTGATGATGT TCCCGAGCGG AGCGAGCGAC GAGACCCTGA TGTGGCTTCT AGGCCGACTT 
CGGGCTGGAA CCCCGGGCTT GGTGGTGCAC GTGCGACACC ATGCCTCTTC AGATAGTTAC 
AGCTTTTACT TAACTGCGCC GTTTAGCGTA CTTTTGAAAG CAGCTGAGGA GGTGCATTTA 
CCGAAGACGC TGCGACAGGA GTTCGGCGGT GGACTGAAGG AGTTCGTTGG CTCCGAGGCG 
AGCTGCTTCG AGGGTAGTGA CGATGAAGCT CAGTTCTTCA CCACCCAGGA GAGGCAATCG 
CTGGTACTCC ACCTGCTGCA CACTCTCAGG GCGGGCCCAC AGGACCTCCA CAGCCTGGCC 
GGGCTCAAGA TGGTCGAAGG TCAAGCAATT ATTCCAAAGT GCATTTCAGC TGGGATCATA 
TCCCAGGTCT TCCCTCTTCA CGAATTGCCT GCGTTGGAGA AACTGCAACG GACATGGGTT 
CGTGCGTTTC TCAGCCCCCA ACCCCTGGAC GACATTTGTA AATACTTCGG AGTCAAGATT 
ACCATGTATT TCGCGTGGCT CGGTCATTAC ACCACCGCTT TGATAGTTCC AGCTGTAGTG 
GGTGCCATAT ATTGGATCGG CATCATCGGT AGGAATCAAG CAGTGGAGGA CGTGGCGTAC 
GTACTGTTCT CTGTGTTCAA CGTAATCTGG GCCACCGTTT ACCTGGAAAC TTGGAAAAGA 
AGGGGTGCCG AACTGGCCTA CAGGTGGGGC ACGTTGGATC AAAGGGACGA CCTACTTCTC 
GAACCTAGGC CCTTATTCAC GGGTACCTTG GAAATTTCAT CGGTGACTGG AAGACTGGAG 
CCGACGTACC CTAGATGGAA GAGGAACATG TTCCGGTATT TCGTCAGCGT GCCCATCATT 
GCAGCGTGCC TGTTCTTCGT TTTCATCGTG ATGATTCTCA GCTTTCAGAT ACAGGATTGG 
TGGGACGCAC GCCTCGAGTC TAGGGGATAC GGTTTCTGGT TGAGCTACGT GCCAAAAGTC 
TTACTCGCGG TGGTGATAGC TTTAATGGAC GAGGCTTACT TCAAGGTTGC CGTATGGTTG 
AACGACATGG AAAACTATCG GCTAGACACC GAGTACGAGA ATCATCTGAT CTACAAGGTG 
GCACTGTTTC AGTTTGTAAA CTCCTTCCTA TCGCTATTTT ACATCGCCTT CTACCTACAG 
GATCAGGAGA GGCTGAAAGA GCAATTGGCC GCTCTGCTGA TAGTCCGGCA GGTGATCGGG 
CATCTGAAAG AGTCTGCAGT GCCGTATCTG ATCGAACAGC TACGACTCGC AAGGTTGAGC 
TTCGAGCTTT TCGGCGCGCT GAGCCCCAGT GAGGCTCGTC CTCCTCCTGG CGAGGACACC 
GAGGAAAATG GGAAGCAGGA GGGCGGCGAT GAGAAGAACG ATCGACCGGA CAGTGCGAAA 
GCGAAGCAGA GAAACGTCAG CCAGGCCGAG CTGGAGAGTT CCCTCTACAG ATACGACGGG 
GCGTTTTCAG AGCATCTGGA AATGCTGTCG CAACTGGGAT ACGTCTGCCT GTTCTCCTCG 
GCTTTCCCTC TGGCAGCGAT GTTAGCGTTG CTTGGAAATC TTTTTGAGCT ACGAGGCGAC 
GCTTTCAAGC TGTGCTTTGT ACTTCAGCGA CCGTTCGGCC GTCGGGTCTC CAGCATTGGA 
ACTTGGCAGA ACGCCATGGA GGCGATGGGT CTGGTCGCTA TTTTGGTGAA CTGCGCGCTG 
ATCGGTCTCA GCGGGCAGGT TCAACGGATG TTCCCGGAGA TGTCGGCCAC TCAGACGATT 
CTGTTGATAG TTGCCCTTGA ACATATCATG CTGGCGATAC GATTCATCAT AATCTGCGCG 
ATTCCCGACA TACCGCATTG GGTGGCCACG GAAATGGCCA AGGTAGAGTT CCTGAGGAGA 
GAGGCGGTCA GAAGGTTATC CTCAACGCCA TCACCGGAGC AGCAACCGGC TACAGTGATA 
GGGAGATTCG TGGTGAGTCC AGCAGACGGT GAGAGCGAAG AGCAACAATT GTTGTCAGAG 
GGCAACGGCG ACGCGACGCC CTCCAGCATC CCGGACACGC CGACGCTGGC GTCGACCACC 
CAGACGCCGA GCAGCCCTCC AACTCCTAGT GGTGCATCCG TGCCGAGGAT CTCGGAGACA 
CCCTCCGCGG CTCAAACTCC GGTCAGCTGT GCCAGCAGCG ACTTGGGTAG TCCTTTACTG 
ACCGACAAGA GCATCATAGG CAGCGTCCGA GACATTCACC ACGCGCCTAG GTGTTCCATT 
CCCGGTGGAG AACGAGGAAG GCGTTCAAGG GACTGGCTAC AGACTGAATC GGAAGCGTCG 
GGTGGTGGCG ATCAGTACAG CCACCATCTG ACGATCGGTC CTCACGGTGG TATAGACTGG 
GTCCGAAGAT TAGGTCTCGA GACTGCTGTT CGAAAATCCA GCGACTCCGA GATCAGCGGA 
GCTGGTACCG TCAGTGGAAG TGGAGACGAG TTGACTCTTC ACAGGTCTAC TGACTGCATC 
GTCTCGAAAG AACTCGCATC CTCATCGGAT AGCGATCTCC TCAGATCCGC CCCGCCATGG 
TCAGTGGCTC ACAGGAATCA GAAGTTCCGT TTCTCGCCCG AACGAGAGAA GGAACGGGAG 
AAGACCGAGA AGCAGCAGTA TCAACAGTAT CAGCGTGAAA TTCAAGATCA CAAGCAACAA 
GCAGCGTTGT ACGCTGAAAA AGAGAAGGAG AAAGAGTCAA GTGCCCTCTC AGACTCGAGC 
AAAACTTCCA GTCAGGAGGA GGAGAAGCCG TCGAAGGAGG ATCGAGAGGC GAAGAAGACC 
AGGGTGAAGC AGAGTTTAAT GAAAAGAGCC AGGTCCGTGG CGATCTTCTC GTTAAAGCTG 
AAGGAGCGGC GCGCGAGGGA GGCCGAGATG AAGGCGAAGG AGGCGGAGAG GGAAGCCAGG 
TGGCAACAGC CGCAGTCCTG CGTCGGCGGC GAACTCTCTT GCATTCCCAT AGAAAAGCTT 
ATATCTGTGG ACGACATCGC AGCCATGGAG TTACGCAGAC TTAATCATTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017885927.1
CDS	96..3446
Translation	MPGGEGSPINLLDQHRASTTSGECASTSDDDEKTATGTVSSTMTTVFAGDGLRRRKVIHAAKETLDKASRLLRRKIPCTGHLMTPRRLWLQKVPTQECDVVMMFPSGASDETLMWLLGRLRAGTPGLVVHVRHHASSDSYSFYLTAPFSVLLKAAEEVHLPKTLRQEFGGGLKEFVGSEASCFEGSDDEAQFFTTQERQSLVLHLLHTLRAGPQDLHSLAGLKMVEGQAIIPKCISAGIISQVFPLHELPALEKLQRTWVRAFLSPQPLDDICKYFGVKITMYFAWLGHYTTALIVPAVVGAIYWIGIIGRNQAVEDVAYVLFSVFNVIWATVYLETWKRRGAELAYRWGTLDQRDDLLLEPRPLFTGTLEISSVTGRLEPTYPRWKRNMFRYFVSVPIIAACLFFVFIVMILSFQIQDWWDARLESRGYGFWLSYVPKVLLAVVIALMDEAYFKVAVWLNDMENYRLDTEYENHLIYKVALFQFVNSFLSLFYIAFYLQDQERLKEQLAALLIVRQVIGHLKESAVPYLIEQLRLARLSFELFGALSPSEARPPPGEDTEENGKQEGGDEKNDRPDSAKAKQRNVSQAELESSLYRYDGAFSEHLEMLSQLGYVCLFSSAFPLAAMLALLGNLFELRGDAFKLCFVLQRPFGRRVSSIGTWQNAMEAMGLVAILVNCALIGLSGQVQRMFPEMSATQTILLIVALEHIMLAIRFIIICAIPDIPHWVATEMAKVEFLRREAVRRLSSTPSPEQQPATVIGRFVVSPADGESEEQQLLSEGNGDATPSSIPDTPTLASTTQTPSSPPTPSGASVPRISETPSAAQTPVSCASSDLGSPLLTDKSIIGSVRDIHHAPRCSIPGGERGRRSRDWLQTESEASGGGDQYSHHLTIGPHGGIDWVRRLGLETAVRKSSDSEISGAGTVSGSGDELTLHRSTDCIVSKELASSSDSDLLRSAPPWSVAHRNQKFRFSPEREKEREKTEKQQYQQYQREIQDHKQQAALYAEKEKEKESSALSDSSKTSSQEEEKPSKEDREAKKTRVKQSLMKRARSVAIFSLKLKERRAREAEMKAKEAEREARWQQPQSCVGGELSCIPIEKLISVDDIAAMELRRLNH

Target ORF information:

RefSeq Version	XM_018030438.2
Organism	Ceratina calcarata
Definition	Ceratina calcarata anoctamin-8-like (LOC108628482), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018030438.2

ORF Insert Sequence:

ATGCCAGGCG GCGAGGGGTC CCCGATCAAT CTCCTCGACC AGCATCGTGC ATCGACCACC 
TCCGGCGAGT GCGCGAGTAC AAGTGACGAT GATGAGAAGA CTGCCACGGG CACCGTTTCC 
AGCACGATGA CGACCGTCTT CGCGGGCGAC GGTCTCAGAC GGAGGAAGGT CATACACGCG 
GCCAAAGAAA CCCTCGACAA GGCTTCGCGT CTGCTCAGAC GGAAGATACC ATGCACAGGC 
CACTTGATGA CCCCCCGCCG CCTATGGTTA CAAAAGGTTC CAACGCAGGA ATGCGACGTC 
GTGATGATGT TCCCGAGCGG AGCGAGCGAC GAGACCCTGA TGTGGCTTCT AGGCCGACTT 
CGGGCTGGAA CCCCGGGCTT GGTGGTGCAC GTGCGACACC ATGCCTCTTC AGATAGTTAC 
AGCTTTTACT TAACTGCGCC GTTTAGCGTA CTTTTGAAAG CAGCTGAGGA GGTGCATTTA 
CCGAAGACGC TGCGACAGGA GTTCGGCGGT GGACTGAAGG AGTTCGTTGG CTCCGAGGCG 
AGCTGCTTCG AGGGTAGTGA CGATGAAGCT CAGTTCTTCA CCACCCAGGA GAGGCAATCG 
CTGGTACTCC ACCTGCTGCA CACTCTCAGG GCGGGCCCAC AGGACCTCCA CAGCCTGGCC 
GGGCTCAAGA TGGTCGAAGG TCAAGCAATT ATTCCAAAGT GCATTTCAGC TGGGATCATA 
TCCCAGGTCT TCCCTCTTCA CGAATTGCCT GCGTTGGAGA AACTGCAACG GACATGGGTT 
CGTGCGTTTC TCAGCCCCCA ACCCCTGGAC GACATTTGTA AATACTTCGG AGTCAAGATT 
ACCATGTATT TCGCGTGGCT CGGTCATTAC ACCACCGCTT TGATAGTTCC AGCTGTAGTG 
GGTGCCATAT ATTGGATCGG CATCATCGGT AGGAATCAAG CAGTGGAGGA CGTGGCGTAC 
GTACTGTTCT CTGTGTTCAA CGTAATCTGG GCCACCGTTT ACCTGGAAAC TTGGAAAAGA 
AGGGGTGCCG AACTGGCCTA CAGGTGGGGC ACGTTGGATC AAAGGGACGA CCTACTTCTC 
GAACCTAGGC CCTTATTCAC GGGTACCTTG GAAATTTCAT CGGTGACTGG AAGACTGGAG 
CCGACGTACC CTAGATGGAA GAGGAACATG TTCCGGTATT TCGTCAGCGT GCCCATCATT 
GCAGCGTGCC TGTTCTTCGT TTTCATCGTG ATGATTCTCA GCTTTCAGAT ACAGGATTGG 
TGGGACGCAC GCCTCGAGTC TAGGGGATAC GGTTTCTGGT TGAGCTACGT GCCAAAAGTC 
TTACTCGCGG TGGTGATAGC TTTAATGGAC GAGGCTTACT TCAAGGTTGC CGTATGGTTG 
AACGACATGG AAAACTATCG GCTAGACACC GAGTACGAGA ATCATCTGAT CTACAAGGTG 
GCACTGTTTC AGTTTGTAAA CTCCTTCCTA TCGCTATTTT ACATCGCCTT CTACCTACAG 
GATCAGGAGA GGCTGAAAGA GCAATTGGCC GCTCTGCTGA TAGTCCGGCA GGTGATCGGG 
CATCTGAAAG AGTCTGCAGT GCCGTATCTG ATCGAACAGC TACGACTCGC AAGGTTGAGC 
TTCGAGCTTT TCGGCGCGCT GAGCCCCAGT GAGGCTCGTC CTCCTCCTGG CGAGGACACC 
GAGGAAAATG GGAAGCAGGA GGGCGGCGAT GAGAAGAACG ATCGACCGGA CAGTGCGAAA 
GCGAAGCAGA GAAACGTCAG CCAGGCCGAG CTGGAGAGTT CCCTCTACAG ATACGACGGG 
GCGTTTTCAG AGCATCTGGA AATGCTGTCG CAACTGGGAT ACGTCTGCCT GTTCTCCTCG 
GCTTTCCCTC TGGCAGCGAT GTTAGCGTTG CTTGGAAATC TTTTTGAGCT ACGAGGCGAC 
GCTTTCAAGC TGTGCTTTGT ACTTCAGCGA CCGTTCGGCC GTCGGGTCTC CAGCATTGGA 
ACTTGGCAGA ACGCCATGGA GGCGATGGGT CTGGTCGCTA TTTTGGTGAA CTGCGCGCTG 
ATCGGTCTCA GCGGGCAGGT TCAACGGATG TTCCCGGAGA TGTCGGCCAC TCAGACGATT 
CTGTTGATAG TTGCCCTTGA ACATATCATG CTGGCGATAC GATTCATCAT AATCTGCGCG 
ATTCCCGACA TACCGCATTG GGTGGCCACG GAAATGGCCA AGGTAGAGTT CCTGAGGAGA 
GAGGCGGTCA GAAGGTTATC CTCAACGCCA TCACCGGAGC AGCAACCGGC TACAGTGATA 
GGGAGATTCG TGGTGAGTCC AGCAGACGGT GAGAGCGAAG AGCAACAATT GTTGTCAGAG 
GGCAACGGCG ACGCGACGCC CTCCAGCATC CCGGACACGC CGACGCTGGC GTCGACCACC 
CAGACGCCGA GCAGCCCTCC AACTCCTAGT GGTGCATCCG TGCCGAGGAT CTCGGAGACA 
CCCTCCGCGG CTCAAACTCC GGTCAGCTGT GCCAGCAGCG ACTTGGGTAG TCCTTTACTG 
ACCGACAAGA GCATCATAGG CAGCGTCCGA GACATTCACC ACGCGCCTAG GTGTTCCATT 
CCCGGTGGAG AACGAGGAAG GCGTTCAAGG GACTGGCTAC AGACTGAATC GGAAGCGTCG 
GGTGGTGGCG ATCAGTACAG CCACCATCTG ACGATCGGTC CTCACGGTGG TATAGACTGG 
GTCCGAAGAT TAGGTCTCGA GACTGCTGTT CGAAAATCCA GCGACTCCGA GATCAGCGGA 
GCTGGTACCG TCAGTGGAAG TGGAGACGAG TTGACTCTTC ACAGGTCTAC TGACTGCATC 
GTCTCGAAAG AACTCGCATC CTCATCGGAT AGCGATCTCC TCAGATCCGC CCCGCCATGG 
TCAGTGGCTC ACAGGAATCA GAAGTTCCGT TTCTCGCCCG AACGAGAGAA GGAACGGGAG 
AAGACCGAGA AGCAGCAGTA TCAACAGTAT CAGCGTGAAA TTCAAGATCA CAAGCAACAA 
GCAGCGTTGT ACGCTGAAAA AGAGAAGGAG AAAGAGTCAA GTGCCCTCTC AGACTCGAGC 
AAAACTTCCA GTCAGGAGGA GGAGAAGCCG TCGAAGGAGG ATCGAGAGGC GAAGAAGACC 
AGGGTGAAGC AGAGTTTAAT GAAAAGAGCC AGGTCCGTGG CGATCTTCTC GTTAAAGCTG 
AAGGAGCGGC GCGCGAGGGA GGCCGAGATG AAGGCGAAGG AGGCGGAGAG GGAAGCCAGG 
TGGCAACAGC CGCAGTCCTG CGTCGGCGGC GAACTCTCTT GCATTCCCAT AGAAAAGCTT 
ATATCTGTGG ACGACATCGC AGCCATGGAG TTACGCAGAC TTAATCATTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC108628482 ( XM_018030438.1 ) cDNA ORF clone, Ceratina calcarata -> XP_017885927.1 Ceratina calcarata anoctamin-8-like (LOC108628482), transcript variant X4, mRNA.

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CloneID	OCe149342
Clone ID Related Accession (Same CDS sequence)	XM_018030438.2 , XM_018030438.1
Accession Version	XM_018030438.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3351bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-08-30
Organism	Ceratina calcarata
Product	anoctamin-8-like isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017176396.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ceratina calcarata Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAGGCG GCGAGGGGTC CCCGATCAAT CTCCTCGACC AGCATCGTGC ATCGACCACC 
TCCGGCGAGT GCGCGAGTAC AAGTGACGAT GATGAGAAGA CTGCCACGGG CACCGTTTCC 
AGCACGATGA CGACCGTCTT CGCGGGCGAC GGTCTCAGAC GGAGGAAGGT CATACACGCG 
GCCAAAGAAA CCCTCGACAA GGCTTCGCGT CTGCTCAGAC GGAAGATACC ATGCACAGGC 
CACTTGATGA CCCCCCGCCG CCTATGGTTA CAAAAGGTTC CAACGCAGGA ATGCGACGTC 
GTGATGATGT TCCCGAGCGG AGCGAGCGAC GAGACCCTGA TGTGGCTTCT AGGCCGACTT 
CGGGCTGGAA CCCCGGGCTT GGTGGTGCAC GTGCGACACC ATGCCTCTTC AGATAGTTAC 
AGCTTTTACT TAACTGCGCC GTTTAGCGTA CTTTTGAAAG CAGCTGAGGA GGTGCATTTA 
CCGAAGACGC TGCGACAGGA GTTCGGCGGT GGACTGAAGG AGTTCGTTGG CTCCGAGGCG 
AGCTGCTTCG AGGGTAGTGA CGATGAAGCT CAGTTCTTCA CCACCCAGGA GAGGCAATCG 
CTGGTACTCC ACCTGCTGCA CACTCTCAGG GCGGGCCCAC AGGACCTCCA CAGCCTGGCC 
GGGCTCAAGA TGGTCGAAGG TCAAGCAATT ATTCCAAAGT GCATTTCAGC TGGGATCATA 
TCCCAGGTCT TCCCTCTTCA CGAATTGCCT GCGTTGGAGA AACTGCAACG GACATGGGTT 
CGTGCGTTTC TCAGCCCCCA ACCCCTGGAC GACATTTGTA AATACTTCGG AGTCAAGATT 
ACCATGTATT TCGCGTGGCT CGGTCATTAC ACCACCGCTT TGATAGTTCC AGCTGTAGTG 
GGTGCCATAT ATTGGATCGG CATCATCGGT AGGAATCAAG CAGTGGAGGA CGTGGCGTAC 
GTACTGTTCT CTGTGTTCAA CGTAATCTGG GCCACCGTTT ACCTGGAAAC TTGGAAAAGA 
AGGGGTGCCG AACTGGCCTA CAGGTGGGGC ACGTTGGATC AAAGGGACGA CCTACTTCTC 
GAACCTAGGC CCTTATTCAC GGGTACCTTG GAAATTTCAT CGGTGACTGG AAGACTGGAG 
CCGACGTACC CTAGATGGAA GAGGAACATG TTCCGGTATT TCGTCAGCGT GCCCATCATT 
GCAGCGTGCC TGTTCTTCGT TTTCATCGTG ATGATTCTCA GCTTTCAGAT ACAGGATTGG 
TGGGACGCAC GCCTCGAGTC TAGGGGATAC GGTTTCTGGT TGAGCTACGT GCCAAAAGTC 
TTACTCGCGG TGGTGATAGC TTTAATGGAC GAGGCTTACT TCAAGGTTGC CGTATGGTTG 
AACGACATGG AAAACTATCG GCTAGACACC GAGTACGAGA ATCATCTGAT CTACAAGGTG 
GCACTGTTTC AGTTTGTAAA CTCCTTCCTA TCGCTATTTT ACATCGCCTT CTACCTACAG 
GATCAGGAGA GGCTGAAAGA GCAATTGGCC GCTCTGCTGA TAGTCCGGCA GGTGATCGGG 
CATCTGAAAG AGTCTGCAGT GCCGTATCTG ATCGAACAGC TACGACTCGC AAGGTTGAGC 
TTCGAGCTTT TCGGCGCGCT GAGCCCCAGT GAGGCTCGTC CTCCTCCTGG CGAGGACACC 
GAGGAAAATG GGAAGCAGGA GGGCGGCGAT GAGAAGAACG ATCGACCGGA CAGTGCGAAA 
GCGAAGCAGA GAAACGTCAG CCAGGCCGAG CTGGAGAGTT CCCTCTACAG ATACGACGGG 
GCGTTTTCAG AGCATCTGGA AATGCTGTCG CAACTGGGAT ACGTCTGCCT GTTCTCCTCG 
GCTTTCCCTC TGGCAGCGAT GTTAGCGTTG CTTGGAAATC TTTTTGAGCT ACGAGGCGAC 
GCTTTCAAGC TGTGCTTTGT ACTTCAGCGA CCGTTCGGCC GTCGGGTCTC CAGCATTGGA 
ACTTGGCAGA ACGCCATGGA GGCGATGGGT CTGGTCGCTA TTTTGGTGAA CTGCGCGCTG 
ATCGGTCTCA GCGGGCAGGT TCAACGGATG TTCCCGGAGA TGTCGGCCAC TCAGACGATT 
CTGTTGATAG TTGCCCTTGA ACATATCATG CTGGCGATAC GATTCATCAT AATCTGCGCG 
ATTCCCGACA TACCGCATTG GGTGGCCACG GAAATGGCCA AGGTAGAGTT CCTGAGGAGA 
GAGGCGGTCA GAAGGTTATC CTCAACGCCA TCACCGGAGC AGCAACCGGC TACAGTGATA 
GGGAGATTCG TGGTGAGTCC AGCAGACGGT GAGAGCGAAG AGCAACAATT GTTGTCAGAG 
GGCAACGGCG ACGCGACGCC CTCCAGCATC CCGGACACGC CGACGCTGGC GTCGACCACC 
CAGACGCCGA GCAGCCCTCC AACTCCTAGT GGTGCATCCG TGCCGAGGAT CTCGGAGACA 
CCCTCCGCGG CTCAAACTCC GGTCAGCTGT GCCAGCAGCG ACTTGGGTAG TCCTTTACTG 
ACCGACAAGA GCATCATAGG CAGCGTCCGA GACATTCACC ACGCGCCTAG GTGTTCCATT 
CCCGGTGGAG AACGAGGAAG GCGTTCAAGG GACTGGCTAC AGACTGAATC GGAAGCGTCG 
GGTGGTGGCG ATCAGTACAG CCACCATCTG ACGATCGGTC CTCACGGTGG TATAGACTGG 
GTCCGAAGAT TAGGTCTCGA GACTGCTGTT CGAAAATCCA GCGACTCCGA GATCAGCGGA 
GCTGGTACCG TCAGTGGAAG TGGAGACGAG TTGACTCTTC ACAGGTCTAC TGACTGCATC 
GTCTCGAAAG AACTCGCATC CTCATCGGAT AGCGATCTCC TCAGATCCGC CCCGCCATGG 
TCAGTGGCTC ACAGGAATCA GAAGTTCCGT TTCTCGCCCG AACGAGAGAA GGAACGGGAG 
AAGACCGAGA AGCAGCAGTA TCAACAGTAT CAGCGTGAAA TTCAAGATCA CAAGCAACAA 
GCAGCGTTGT ACGCTGAAAA AGAGAAGGAG AAAGAGTCAA GTGCCCTCTC AGACTCGAGC 
AAAACTTCCA GTCAGGAGGA GGAGAAGCCG TCGAAGGAGG ATCGAGAGGC GAAGAAGACC 
AGGGTGAAGC AGAGTTTAAT GAAAAGAGCC AGGTCCGTGG CGATCTTCTC GTTAAAGCTG 
AAGGAGCGGC GCGCGAGGGA GGCCGAGATG AAGGCGAAGG AGGCGGAGAG GGAAGCCAGG 
TGGCAACAGC CGCAGTCCTG CGTCGGCGGC GAACTCTCTT GCATTCCCAT AGAAAAGCTT 
ATATCTGTGG ACGACATCGC AGCCATGGAG TTACGCAGAC TTAATCATTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017885927.1
CDS	64..3414
Translation	MPGGEGSPINLLDQHRASTTSGECASTSDDDEKTATGTVSSTMTTVFAGDGLRRRKVIHAAKETLDKASRLLRRKIPCTGHLMTPRRLWLQKVPTQECDVVMMFPSGASDETLMWLLGRLRAGTPGLVVHVRHHASSDSYSFYLTAPFSVLLKAAEEVHLPKTLRQEFGGGLKEFVGSEASCFEGSDDEAQFFTTQERQSLVLHLLHTLRAGPQDLHSLAGLKMVEGQAIIPKCISAGIISQVFPLHELPALEKLQRTWVRAFLSPQPLDDICKYFGVKITMYFAWLGHYTTALIVPAVVGAIYWIGIIGRNQAVEDVAYVLFSVFNVIWATVYLETWKRRGAELAYRWGTLDQRDDLLLEPRPLFTGTLEISSVTGRLEPTYPRWKRNMFRYFVSVPIIAACLFFVFIVMILSFQIQDWWDARLESRGYGFWLSYVPKVLLAVVIALMDEAYFKVAVWLNDMENYRLDTEYENHLIYKVALFQFVNSFLSLFYIAFYLQDQERLKEQLAALLIVRQVIGHLKESAVPYLIEQLRLARLSFELFGALSPSEARPPPGEDTEENGKQEGGDEKNDRPDSAKAKQRNVSQAELESSLYRYDGAFSEHLEMLSQLGYVCLFSSAFPLAAMLALLGNLFELRGDAFKLCFVLQRPFGRRVSSIGTWQNAMEAMGLVAILVNCALIGLSGQVQRMFPEMSATQTILLIVALEHIMLAIRFIIICAIPDIPHWVATEMAKVEFLRREAVRRLSSTPSPEQQPATVIGRFVVSPADGESEEQQLLSEGNGDATPSSIPDTPTLASTTQTPSSPPTPSGASVPRISETPSAAQTPVSCASSDLGSPLLTDKSIIGSVRDIHHAPRCSIPGGERGRRSRDWLQTESEASGGGDQYSHHLTIGPHGGIDWVRRLGLETAVRKSSDSEISGAGTVSGSGDELTLHRSTDCIVSKELASSSDSDLLRSAPPWSVAHRNQKFRFSPEREKEREKTEKQQYQQYQREIQDHKQQAALYAEKEKEKESSALSDSSKTSSQEEEKPSKEDREAKKTRVKQSLMKRARSVAIFSLKLKERRAREAEMKAKEAEREARWQQPQSCVGGELSCIPIEKLISVDDIAAMELRRLNH

Target ORF information:

RefSeq Version	XM_018030438.1
Organism	Ceratina calcarata
Definition	Ceratina calcarata anoctamin-8-like (LOC108628482), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018030438.1

ORF Insert Sequence:

ATGCCAGGCG GCGAGGGGTC CCCGATCAAT CTCCTCGACC AGCATCGTGC ATCGACCACC 
TCCGGCGAGT GCGCGAGTAC AAGTGACGAT GATGAGAAGA CTGCCACGGG CACCGTTTCC 
AGCACGATGA CGACCGTCTT CGCGGGCGAC GGTCTCAGAC GGAGGAAGGT CATACACGCG 
GCCAAAGAAA CCCTCGACAA GGCTTCGCGT CTGCTCAGAC GGAAGATACC ATGCACAGGC 
CACTTGATGA CCCCCCGCCG CCTATGGTTA CAAAAGGTTC CAACGCAGGA ATGCGACGTC 
GTGATGATGT TCCCGAGCGG AGCGAGCGAC GAGACCCTGA TGTGGCTTCT AGGCCGACTT 
CGGGCTGGAA CCCCGGGCTT GGTGGTGCAC GTGCGACACC ATGCCTCTTC AGATAGTTAC 
AGCTTTTACT TAACTGCGCC GTTTAGCGTA CTTTTGAAAG CAGCTGAGGA GGTGCATTTA 
CCGAAGACGC TGCGACAGGA GTTCGGCGGT GGACTGAAGG AGTTCGTTGG CTCCGAGGCG 
AGCTGCTTCG AGGGTAGTGA CGATGAAGCT CAGTTCTTCA CCACCCAGGA GAGGCAATCG 
CTGGTACTCC ACCTGCTGCA CACTCTCAGG GCGGGCCCAC AGGACCTCCA CAGCCTGGCC 
GGGCTCAAGA TGGTCGAAGG TCAAGCAATT ATTCCAAAGT GCATTTCAGC TGGGATCATA 
TCCCAGGTCT TCCCTCTTCA CGAATTGCCT GCGTTGGAGA AACTGCAACG GACATGGGTT 
CGTGCGTTTC TCAGCCCCCA ACCCCTGGAC GACATTTGTA AATACTTCGG AGTCAAGATT 
ACCATGTATT TCGCGTGGCT CGGTCATTAC ACCACCGCTT TGATAGTTCC AGCTGTAGTG 
GGTGCCATAT ATTGGATCGG CATCATCGGT AGGAATCAAG CAGTGGAGGA CGTGGCGTAC 
GTACTGTTCT CTGTGTTCAA CGTAATCTGG GCCACCGTTT ACCTGGAAAC TTGGAAAAGA 
AGGGGTGCCG AACTGGCCTA CAGGTGGGGC ACGTTGGATC AAAGGGACGA CCTACTTCTC 
GAACCTAGGC CCTTATTCAC GGGTACCTTG GAAATTTCAT CGGTGACTGG AAGACTGGAG 
CCGACGTACC CTAGATGGAA GAGGAACATG TTCCGGTATT TCGTCAGCGT GCCCATCATT 
GCAGCGTGCC TGTTCTTCGT TTTCATCGTG ATGATTCTCA GCTTTCAGAT ACAGGATTGG 
TGGGACGCAC GCCTCGAGTC TAGGGGATAC GGTTTCTGGT TGAGCTACGT GCCAAAAGTC 
TTACTCGCGG TGGTGATAGC TTTAATGGAC GAGGCTTACT TCAAGGTTGC CGTATGGTTG 
AACGACATGG AAAACTATCG GCTAGACACC GAGTACGAGA ATCATCTGAT CTACAAGGTG 
GCACTGTTTC AGTTTGTAAA CTCCTTCCTA TCGCTATTTT ACATCGCCTT CTACCTACAG 
GATCAGGAGA GGCTGAAAGA GCAATTGGCC GCTCTGCTGA TAGTCCGGCA GGTGATCGGG 
CATCTGAAAG AGTCTGCAGT GCCGTATCTG ATCGAACAGC TACGACTCGC AAGGTTGAGC 
TTCGAGCTTT TCGGCGCGCT GAGCCCCAGT GAGGCTCGTC CTCCTCCTGG CGAGGACACC 
GAGGAAAATG GGAAGCAGGA GGGCGGCGAT GAGAAGAACG ATCGACCGGA CAGTGCGAAA 
GCGAAGCAGA GAAACGTCAG CCAGGCCGAG CTGGAGAGTT CCCTCTACAG ATACGACGGG 
GCGTTTTCAG AGCATCTGGA AATGCTGTCG CAACTGGGAT ACGTCTGCCT GTTCTCCTCG 
GCTTTCCCTC TGGCAGCGAT GTTAGCGTTG CTTGGAAATC TTTTTGAGCT ACGAGGCGAC 
GCTTTCAAGC TGTGCTTTGT ACTTCAGCGA CCGTTCGGCC GTCGGGTCTC CAGCATTGGA 
ACTTGGCAGA ACGCCATGGA GGCGATGGGT CTGGTCGCTA TTTTGGTGAA CTGCGCGCTG 
ATCGGTCTCA GCGGGCAGGT TCAACGGATG TTCCCGGAGA TGTCGGCCAC TCAGACGATT 
CTGTTGATAG TTGCCCTTGA ACATATCATG CTGGCGATAC GATTCATCAT AATCTGCGCG 
ATTCCCGACA TACCGCATTG GGTGGCCACG GAAATGGCCA AGGTAGAGTT CCTGAGGAGA 
GAGGCGGTCA GAAGGTTATC CTCAACGCCA TCACCGGAGC AGCAACCGGC TACAGTGATA 
GGGAGATTCG TGGTGAGTCC AGCAGACGGT GAGAGCGAAG AGCAACAATT GTTGTCAGAG 
GGCAACGGCG ACGCGACGCC CTCCAGCATC CCGGACACGC CGACGCTGGC GTCGACCACC 
CAGACGCCGA GCAGCCCTCC AACTCCTAGT GGTGCATCCG TGCCGAGGAT CTCGGAGACA 
CCCTCCGCGG CTCAAACTCC GGTCAGCTGT GCCAGCAGCG ACTTGGGTAG TCCTTTACTG 
ACCGACAAGA GCATCATAGG CAGCGTCCGA GACATTCACC ACGCGCCTAG GTGTTCCATT 
CCCGGTGGAG AACGAGGAAG GCGTTCAAGG GACTGGCTAC AGACTGAATC GGAAGCGTCG 
GGTGGTGGCG ATCAGTACAG CCACCATCTG ACGATCGGTC CTCACGGTGG TATAGACTGG 
GTCCGAAGAT TAGGTCTCGA GACTGCTGTT CGAAAATCCA GCGACTCCGA GATCAGCGGA 
GCTGGTACCG TCAGTGGAAG TGGAGACGAG TTGACTCTTC ACAGGTCTAC TGACTGCATC 
GTCTCGAAAG AACTCGCATC CTCATCGGAT AGCGATCTCC TCAGATCCGC CCCGCCATGG 
TCAGTGGCTC ACAGGAATCA GAAGTTCCGT TTCTCGCCCG AACGAGAGAA GGAACGGGAG 
AAGACCGAGA AGCAGCAGTA TCAACAGTAT CAGCGTGAAA TTCAAGATCA CAAGCAACAA 
GCAGCGTTGT ACGCTGAAAA AGAGAAGGAG AAAGAGTCAA GTGCCCTCTC AGACTCGAGC 
AAAACTTCCA GTCAGGAGGA GGAGAAGCCG TCGAAGGAGG ATCGAGAGGC GAAGAAGACC 
AGGGTGAAGC AGAGTTTAAT GAAAAGAGCC AGGTCCGTGG CGATCTTCTC GTTAAAGCTG 
AAGGAGCGGC GCGCGAGGGA GGCCGAGATG AAGGCGAAGG AGGCGGAGAG GGAAGCCAGG 
TGGCAACAGC CGCAGTCCTG CGTCGGCGGC GAACTCTCTT GCATTCCCAT AGAAAAGCTT 
ATATCTGTGG ACGACATCGC AGCCATGGAG TTACGCAGAC TTAATCATTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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03-6811-6572

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LOC108628482 cDNA ORF clone, Ceratina calcarata

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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見積依頼

Eメール

電話

FAX

オンラインフォーム