DENND4A gene cDNA ORF clone, Canis lupus familiaris(dog)

mRNA	Protein	Name
XM_005638528.2	XP_005638585.1	C-myc promoter-binding protein isoform X2
XM_005638527.3	XP_005638584.1	C-myc promoter-binding protein isoform X1
XM_022412983.1	XP_022268691.1	C-myc promoter-binding protein isoform X1
XM_005638527.2	XP_005638584.1	C-myc promoter-binding protein isoform X1
XM_003433926.3	XP_003433974.1	C-myc promoter-binding protein isoform X4
XM_003433926.4	XP_003433974.1	C-myc promoter-binding protein isoform X4
XM_005638530.2	XP_005638587.1	C-myc promoter-binding protein isoform X5
XM_022412984.1	XP_022268692.1	C-myc promoter-binding protein isoform X1
XM_005638529.3	XP_005638586.1	C-myc promoter-binding protein isoform X3
XM_544733.6	XP_544733.3	C-myc promoter-binding protein isoform X6
XM_005638529.2	XP_005638586.1	C-myc promoter-binding protein isoform X3
XM_544733.5	XP_544733.3	C-myc promoter-binding protein isoform X6
XM_005638528.3	XP_005638585.1	C-myc promoter-binding protein isoform X2
XM_005638530.3	XP_005638587.1	C-myc promoter-binding protein isoform X5

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe29018	XM_005638527.3 Latest version!	Canis lupus familiaris DENN domain containing 4A (DENND4A), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OCe29018	XM_022412984.1 Latest version!	Canis lupus familiaris DENN domain containing 4A (DENND4A), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OCe29018	XM_022412983.1 Latest version!	Canis lupus familiaris DENN domain containing 4A (DENND4A), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OCe29019	XM_005638528.3 Latest version!	Canis lupus familiaris DENN domain containing 4A (DENND4A), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OCe29020	XM_005638529.3 Latest version!	Canis lupus familiaris DENN domain containing 4A (DENND4A), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OCe29021	XM_003433926.4 Latest version!	Canis lupus familiaris DENN domain containing 4A (DENND4A), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OCe29022	XM_005638530.3 Latest version!	Canis lupus familiaris DENN domain containing 4A (DENND4A), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OCe29023	XM_544733.6 Latest version!	Canis lupus familiaris DENN domain containing 4A (DENND4A), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
View Old Accession Versions >>
OCe29018	XM_005638527.2	Canis lupus familiaris DENN/MADD domain containing 4A (DENND4A), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OCe29021	XM_003433926.3	Canis lupus familiaris DENN/MADD domain containing 4A (DENND4A), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OCe29020	XM_005638529.2	Canis lupus familiaris DENN/MADD domain containing 4A (DENND4A), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OCe29023	XM_544733.5	Canis lupus familiaris DENN/MADD domain containing 4A (DENND4A), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OCe29019	XM_005638528.2	Canis lupus familiaris DENN/MADD domain containing 4A (DENND4A), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OCe29022	XM_005638530.2	Canis lupus familiaris DENN/MADD domain containing 4A (DENND4A), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
Hide Old Accession Versions >>

DENND4A ( XM_005638527.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005638584.1 Canis lupus familiaris DENN domain containing 4A (DENND4A), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe29018
Clone ID Related Accession (Same CDS sequence)	XM_005638527.3 , XM_022412983.1 , XM_005638527.2 , XM_022412984.1
Accession Version	XM_005638527.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5757bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	C-myc promoter-binding protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006612.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005638527.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTGAAG ATAAGGGTCC TCGTGTTGCT GACTACTTTG TTGTAGCAGG ATTAACTGAT 
GTTTCAAAGC CTCTTGAAGA AGAAATTCAC TTCAATGATG CCTGTCATAA AGTAGCTAAA 
CCAAAAGAAC CTATCACAGA TGTTTCGGTG ATTATTAAAT CTCTTGGGGA AGAAGTGCCA 
CGGGATTATA TATGTATTGA TGTTACCCCA ACTGGATTGT CGGCTGATCT CAATAATGGA 
AGTCTTGTGG GGCCACAGAT TTACCTTTGC TACAGAAGAG GAAGAGATAA GCCTCCACTT 
ACAGATCTGG GGGTTTTGTA TGACTGGAAA GAAAGATTGA AACAGGGATG TGAAATTATT 
CAGAGTACTC CCTATGGACA CCCTGCAAAT ATTAGTGGCA GTACCTCATC ACAAAAAATT 
TATATAACTT ACCGAAGAGC CTCTGAAAAC ATGACGCAAA ATACGTTGGC TGTCACAGAC 
ATATGTATTA TTATACCCAG TAAAGGAGAG AGTCCACCAC ACACGTTTTG CAAAGTCGAT 
AAGAATCTCA ATAACAGTAT GTGGGGATCT GCAGTGTACT TATGTTATAA AAAATCAGTG 
GCAAAGACTA ACACTATATC CTATAAAGCT GGTCTAATTT GTAGATATCC TCAAGAAGAT 
TACGAATCTT TCTCACTCCC AGAATCTGTT CCCCTATTTT GTTTACCAAT GGGTGCAACT 
ATTGAATGTT GGCCACCAAA TAGCAAATAC CCTCTCCCTG TATTTTCTAC ATTTGTGTTA 
ACTGGAGCCT CAGCTGAAAA GGTCTATGGT GCTGCTATTC AGTTTTATGA ACCATACTCT 
GAGGAGAATC TCACAGAAAA ACAGAGACTT CTTTTGGGTT TAGCATCATC AGCAGATGGG 
AAATCTGATA GTTCTAAAAC AATTCACACT AGTAAATGCA TCTGTCTTCT TTCTCACTGG 
CCTTTTTTTG ATGCATTCAG GAAATTTTTG ACCTTTCTGT ATCGTTACTC TATCTCTGGA 
CCTCATGTCC TTCCAATTGA GAAGCATATT TCTCATTTTA TGCATAAAGT TCCTTTTCCA 
TCTCCTCAGA GGCCACGGAT TTTAGTTCAG TTATCGCCAC ATGATAATCT GATTCTCAGT 
CAACCTGTTT CTTCACCTCT TCCACTAAGT GGTGGCAAAT TTTCAACACT ACTTCAAAAT 
TTAGGCCCTG AAAATGCTGT AACACTACTG GTGTTTGCAG TAACAGAGCA CAAGATTCTC 
ATCCATTCCT TACGGCCTTC CGTGCTTACT AGCGTGACAG AAGCATTAGT GTCTATGATT 
TTCCCTTTCC ACTGGCCGTG CCCATATGTT CCTCTCTGCC CACTGGCTTT AGCAGATGTC 
TTGAGTGCAC CATGTCCATT CATTGTAGGA ATTGATTCAA GATATTTTGA TCTCTATGAC 
CCTCCACCAG ATGTTAGTTG TGTTGACCTA GATACCAACA CTATTTCTCA AACTGGCGAT 
AAGAAAAATG TAGCCTGGAA GATACTTCCA AAGAAACCAT GTAAAAACCT GATGAACACA 
CTGAATAATT TGCACCAGCA GTTGGCAAAA TTGCAGCAGA GACCAAGAGA TGATGGACTA 
ATGGACTTAG CAATGAATGA CTATGATTTT AATTCTGGAA AGAGGTTGCA TATGATAGAT 
TTGGAAATCC AAGAGGCATT TTTGTGTTTC ATGGCCTCTA TTTTAAAAGG TTATAGATCA 
TACTTAAGAC CAATAACACA AGCCCCTTCT GAGACAGCCA CCGATGCATC CTCTCTTTTT 
GCCCTACAAG CTTTCTTAAG AAGCCGAGAC CGGTCACATC AAAAATTCTA TAACATGATG 
ACCAAAACAC AAATGTTCAT TCGCTTCATT GAGGAATGTT CTTTTGTCAG TGATAAAGAT 
GCAAGCCTGG CATTTTTTGA TGATTGTGTA GATAAAGTGG ATATGGACAA GAGTGGTGAA 
ATGCGACTTA TAGAACTGGA TGAATCTTTC AAAAGTGAAC ATACTGTTTT TGTAACACCA 
CCTGAGATAC CCCATCTACC CAATGGTGAA GAACCCCCTT TACAGTACAG CTATAATGGA 
TTTCCAGTTC TTAGAAACAA TTTATTTGAG AGACCTGAAG GATTTCTACA AACACGAAAG 
AACAAATTGC CTTCAAAATC AAGTAGTCCT AGTAGCCCTT CTCCCATGTT CAGAAGAACA 
AAACAGGAAA TTAAATCAGC CCATAAAATT GCCAAGAGGT ACTCTTCCAT TCCTCAGATG 
TGGTCTAGGT GTCTGCTGCG CCACTGCTAT GGACTGTGGT TCATTTGTCT GCCAGCTTAT 
GTGAAAGTGT GTCATTCAAA AGTCAGGGCA CTGAAGACAG CATATGATGT GCTCAAAAAA 
ATGCAGTCGA AAAAGATGGA TCCACCTGAT GAAGTGTGCT ACCGCATTCT CATGCAGCTC 
TGTGGGCAGT ACGATCAGCC CGTGCTTGCA GTTCGAGTGC TTTTTGAAAT GCAGAAAGCT 
GGTATTGATC CCAATGCCAT TACTTATGGT TATTATAATA AGGCTGTTTT GGAAAGCACC 
TGGCCCTCAA GAAGTCGTAG TGGCTATTTT CTTTGGACAA AAGTAAGAAA CGTTGTTTTA 
GGAGTAGCAC AGTTCAAAAG AGCTTTAAAG AAACAGGCAC ACTTATCACA AACAACTCTC 
TCAGCAGATG GCAGTGACCT GGATGCTGTT AGTCATGGCA GCATGGATAG TGGTCATGGG 
ACACACACTG TGGAGCAGGC ACCTTTTAAT ACGGGTTTAA TCAAAGTATA TGCTACTGAT 
GATAGATCTA GTACAGGTGG CCAGTCTGAT CTTGGATATA ATTCATTATC TAAGGATGAA 
GTTAGAAGAG GGGATATATC TACTGAGGAA ATTCAAGAAG AAAAAGATAA GAAAGGAAGT 
GATTCTAGTT CCCTGTCAGA GAGTGAGAGT ACAAAAGGAA GTGCTGACTG TCTTCCTAAA 
CTCAATTATC AAAGTTCTTC CAGTATAGTT CGCCTCAGTG GTACAAATAA CAATGGTGCT 
GATAAAATAT CAGGAGAAAG CACAGAAAGC ACATCTAAGC TGCTCTTACT ATCATCTTTT 
GAGGGTACAA ATGAAACAGG AAATAGTCAA AATAGATGCT TCAGGAAAAG ACATAAAAGT 
GACAACGAAC CTAATTTGCA ACAGCGACTC TCCTGGGGAA GCAGAAACCG CAATCTTAGT 
GGAGGAGTGC TGATGGGACT TATGCTTAAT AGAATCAACC AGGAAGCAAA CCCTGGAGAC 
ATGGTTGAAA AACTAGGAGC TGATGCAAAA ATTCTTTCAA ACGTTATATC ACAAAGCACA 
AGACCAACTA GTCTTGATAT TGGAAAACCC CCTTTAAGAT CAAAAAGAGA CAGTCTAGAA 
AAGGAATCTA GCGACGATGA TACACCTTTC GATGGTTCTA ATTGTTTGGC AGATAAAGTG 
GATTCTCCTG TTATTTTTGA TTTGGAAGAC TTAGACAGTG AGACAGATGG ATCAAAAGTG 
GGATACATTG CTTCACAAAA TCCCAGAAGG ATTCAGCGTA TGAGCAGCAG CTTCTCGGTA 
AAACCTTCTG AAAAAACGGA TGTTGCCACA GGATTTGATC CCCTCTCTCT TTTGGTTGCT 
GAGACTGAAC AGCAGCAAAA AGAGGAAGAG GAGGAGGATG ATGAAGATAG CAAAAGCGTT 
TCAACACCAT CCGCCAGGCG TGACTTAGCC GAAGAGATTG TGATGTATAT GAATAACATG 
AGCAGTCCTT TGACAAGCCG CACACCAAGC ATCGATTTAC AACGGGCATG TGATGACAAA 
TCAACCAGTA AGAAAAGTCC AACATTTGTC AAGGCATGCA GGAGATCTAG CCTGCCTCCT 
CATTCTCCGA GGCCAGTGAG ACTTACCAAA TCTAAAAGTT ATACAAAAAC TGAGGAGAGA 
CCTCGGGACA GACTCTGGTC TTCTCCAGCC TTCTCACCTA CTTGCCCATT TAGGGAAGAA 
TCTCAAGACG CATTAACCCA TTCATCACCT TCATTTAATT TAGATACACT GCTAGTACCT 
AAACTAGATG TTCTAAGGAA CAGTATGTTC ACTGCTGGGA AAGGAGTTGC CGAGAAAGCC 
AGCAAATGGT ATTCAAGATT CACCATGTAT ACCACATCAT CTAAGGATCA AAGTTCTGAT 
CGTACCAGTC TTTCTTCAGT GGGAGCCCAG GATTCTGAAT CTACCTCTTT AACAGATGAA 
GATGTTTGCC ATGAATTGGA AGGAGCCACT TCCTCCCAAG AGAGCTGTGC TGCTTCAGGG 
AATAAGGGAA TTGATTTCAG CAGAACAAGC CTGGAAAGTT CTGCCTCCTT AGAAGGATCC 
CTGTCAAAGT TTGCCTTACC TGGGAAATCA GAAGTGGCAT CTTCCTGCAA CACAAGTAAT 
ACAAATATCT TCCAGAACTA TGCAATGGAG GTTCTTATCT CAAGTTGCTC TCGGTGTAGA 
ACTTGTGATT GTCTTGTCCA TGATGAGGAA ATCATGGCTG GCTGGACAGC AGATGATTCA 
AATCTCAATA CTACATGCCC GTTTTGTGGC AATCTCTTCT TGCCCTTTCT GAATATTGAA 
ATAAGAGATT TAAGACGACC CGGAAGATAC TTTCTGAAGT CAAGTCCATC TACAGAAAAT 
ATGCATTTTC CATCCTCCCT TTCAAGTCAG ACAAGGCAGT CTTGTATCTC AACATCAGCC 
TCTGGTCTTG ACACGTCTGT TATCTCTGTT CAAGGGAATT TTGATCTCAA TAGCAAATCT 
AAACTGCTGG AAAATACATG TGCCCGAACT ATTCACATCC CTGCTGATAG ATCAAAAACA 
GGTGTGTCAA AATGTCCAAT ATTTCCAATG GCCAGGAGTG TTAGTACTTA TGGCCCCTTG 
GATAAGGAAG ACACTGGAGG CCAGAAGCTC ATTCCTACAG GCAGCCTGCC AACTACTTTA 
CAAGGAGCTA CAGATTCTTT AGGATTAGAG TGGCACCTTC CTAGTCCAGA TCCTATCACA 
GTTCCATATC TTAGTCCTTT AGTGGTATGG AAGGAGCTTG AAAGCTTACT GGAAAATGAA 
GGCGACCATG CAATAACAGT GGCAGACTTT GTGGATCATC ATCCAATCGT GTTTTGGAAC 
CTGGTGTGGT ATTTTAGACG TCTCGACTTA CCCAGTAACT TACCTGGATT GATTCTTTCT 
TCTGAGCACT GTAACAAGTA TTCAAAGATT CCTCGCCACT GTATGTCGGA AGATAGTAAA 
TACGTTTTAA TACAAATGTT ATGGGACAAT ATGAAATTAC ATCAGGATCC GGGACAGCCT 
TTGTACATCC TCTGGAATGC CCACAGTCAA AATCGTACTC TTTTGTTTGA GACCCAAAAG 
TATCCAATGG TCCATTTATT GCAAAAGGGT GATAATTCAT TTAACCAGGA ACTGTTGAAA 
AATATGGTAA AAAGCATTAA AATGAATGAT GTCTATGGAC CAATGAGTCA AATTTTAGAG 
ACACTGAATA AATGTCCTCA TTTTAAAAGA CAGAGGAGTT TATATAGAGA AATACTATTT 
CTCTCACTTG TGGCACTGGG AAGAGAAAAC ATTGATATAG ATGCATTTGA TAAAGAATAC 
AAGATGGCAT ACGATCGTCT CACACCTAGT CAAGTCAAGA GTACACACAA CTGTGATAGA 
CCACCAAGTA CAGGGGTGAT GGAATGTCGA AAAACCTTTG GAGAACCTTA TCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005638584.1
CDS	344..6100
Translation	MIEDKGPRVADYFVVAGLTDVSKPLEEEIHFNDACHKVAKPKEPITDVSVIIKSLGEEVPRDYICIDVTPTGLSADLNNGSLVGPQIYLCYRRGRDKPPLTDLGVLYDWKERLKQGCEIIQSTPYGHPANISGSTSSQKIYITYRRASENMTQNTLAVTDICIIIPSKGESPPHTFCKVDKNLNNSMWGSAVYLCYKKSVAKTNTISYKAGLICRYPQEDYESFSLPESVPLFCLPMGATIECWPPNSKYPLPVFSTFVLTGASAEKVYGAAIQFYEPYSEENLTEKQRLLLGLASSADGKSDSSKTIHTSKCICLLSHWPFFDAFRKFLTFLYRYSISGPHVLPIEKHISHFMHKVPFPSPQRPRILVQLSPHDNLILSQPVSSPLPLSGGKFSTLLQNLGPENAVTLLVFAVTEHKILIHSLRPSVLTSVTEALVSMIFPFHWPCPYVPLCPLALADVLSAPCPFIVGIDSRYFDLYDPPPDVSCVDLDTNTISQTGDKKNVAWKILPKKPCKNLMNTLNNLHQQLAKLQQRPRDDGLMDLAMNDYDFNSGKRLHMIDLEIQEAFLCFMASILKGYRSYLRPITQAPSETATDASSLFALQAFLRSRDRSHQKFYNMMTKTQMFIRFIEECSFVSDKDASLAFFDDCVDKVDMDKSGEMRLIELDESFKSEHTVFVTPPEIPHLPNGEEPPLQYSYNGFPVLRNNLFERPEGFLQTRKNKLPSKSSSPSSPSPMFRRTKQEIKSAHKIAKRYSSIPQMWSRCLLRHCYGLWFICLPAYVKVCHSKVRALKTAYDVLKKMQSKKMDPPDEVCYRILMQLCGQYDQPVLAVRVLFEMQKAGIDPNAITYGYYNKAVLESTWPSRSRSGYFLWTKVRNVVLGVAQFKRALKKQAHLSQTTLSADGSDLDAVSHGSMDSGHGTHTVEQAPFNTGLIKVYATDDRSSTGGQSDLGYNSLSKDEVRRGDISTEEIQEEKDKKGSDSSSLSESESTKGSADCLPKLNYQSSSSIVRLSGTNNNGADKISGESTESTSKLLLLSSFEGTNETGNSQNRCFRKRHKSDNEPNLQQRLSWGSRNRNLSGGVLMGLMLNRINQEANPGDMVEKLGADAKILSNVISQSTRPTSLDIGKPPLRSKRDSLEKESSDDDTPFDGSNCLADKVDSPVIFDLEDLDSETDGSKVGYIASQNPRRIQRMSSSFSVKPSEKTDVATGFDPLSLLVAETEQQQKEEEEEDDEDSKSVSTPSARRDLAEEIVMYMNNMSSPLTSRTPSIDLQRACDDKSTSKKSPTFVKACRRSSLPPHSPRPVRLTKSKSYTKTEERPRDRLWSSPAFSPTCPFREESQDALTHSSPSFNLDTLLVPKLDVLRNSMFTAGKGVAEKASKWYSRFTMYTTSSKDQSSDRTSLSSVGAQDSESTSLTDEDVCHELEGATSSQESCAASGNKGIDFSRTSLESSASLEGSLSKFALPGKSEVASSCNTSNTNIFQNYAMEVLISSCSRCRTCDCLVHDEEIMAGWTADDSNLNTTCPFCGNLFLPFLNIEIRDLRRPGRYFLKSSPSTENMHFPSSLSSQTRQSCISTSASGLDTSVISVQGNFDLNSKSKLLENTCARTIHIPADRSKTGVSKCPIFPMARSVSTYGPLDKEDTGGQKLIPTGSLPTTLQGATDSLGLEWHLPSPDPITVPYLSPLVVWKELESLLENEGDHAITVADFVDHHPIVFWNLVWYFRRLDLPSNLPGLILSSEHCNKYSKIPRHCMSEDSKYVLIQMLWDNMKLHQDPGQPLYILWNAHSQNRTLLFETQKYPMVHLLQKGDNSFNQELLKNMVKSIKMNDVYGPMSQILETLNKCPHFKRQRSLYREILFLSLVALGRENIDIDAFDKEYKMAYDRLTPSQVKSTHNCDRPPSTGVMECRKTFGEPYL

Target ORF information:

RefSeq Version	XM_005638527.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris DENN domain containing 4A (DENND4A), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005638527.3

ORF Insert Sequence:

ATGATTGAAG ATAAGGGTCC TCGTGTTGCT GACTACTTTG TTGTAGCAGG ATTAACTGAT 
GTTTCAAAGC CTCTTGAAGA AGAAATTCAC TTCAATGATG CCTGTCATAA AGTAGCTAAA 
CCAAAAGAAC CTATCACAGA TGTTTCGGTG ATTATTAAAT CTCTTGGGGA AGAAGTGCCA 
CGGGATTATA TATGTATTGA TGTTACCCCA ACTGGATTGT CGGCTGATCT CAATAATGGA 
AGTCTTGTGG GGCCACAGAT TTACCTTTGC TACAGAAGAG GAAGAGATAA GCCTCCACTT 
ACAGATCTGG GGGTTTTGTA TGACTGGAAA GAAAGATTGA AACAGGGATG TGAAATTATT 
CAGAGTACTC CCTATGGACA CCCTGCAAAT ATTAGTGGCA GTACCTCATC ACAAAAAATT 
TATATAACTT ACCGAAGAGC CTCTGAAAAC ATGACGCAAA ATACGTTGGC TGTCACAGAC 
ATATGTATTA TTATACCCAG TAAAGGAGAG AGTCCACCAC ACACGTTTTG CAAAGTCGAT 
AAGAATCTCA ATAACAGTAT GTGGGGATCT GCAGTGTACT TATGTTATAA AAAATCAGTG 
GCAAAGACTA ACACTATATC CTATAAAGCT GGTCTAATTT GTAGATATCC TCAAGAAGAT 
TACGAATCTT TCTCACTCCC AGAATCTGTT CCCCTATTTT GTTTACCAAT GGGTGCAACT 
ATTGAATGTT GGCCACCAAA TAGCAAATAC CCTCTCCCTG TATTTTCTAC ATTTGTGTTA 
ACTGGAGCCT CAGCTGAAAA GGTCTATGGT GCTGCTATTC AGTTTTATGA ACCATACTCT 
GAGGAGAATC TCACAGAAAA ACAGAGACTT CTTTTGGGTT TAGCATCATC AGCAGATGGG 
AAATCTGATA GTTCTAAAAC AATTCACACT AGTAAATGCA TCTGTCTTCT TTCTCACTGG 
CCTTTTTTTG ATGCATTCAG GAAATTTTTG ACCTTTCTGT ATCGTTACTC TATCTCTGGA 
CCTCATGTCC TTCCAATTGA GAAGCATATT TCTCATTTTA TGCATAAAGT TCCTTTTCCA 
TCTCCTCAGA GGCCACGGAT TTTAGTTCAG TTATCGCCAC ATGATAATCT GATTCTCAGT 
CAACCTGTTT CTTCACCTCT TCCACTAAGT GGTGGCAAAT TTTCAACACT ACTTCAAAAT 
TTAGGCCCTG AAAATGCTGT AACACTACTG GTGTTTGCAG TAACAGAGCA CAAGATTCTC 
ATCCATTCCT TACGGCCTTC CGTGCTTACT AGCGTGACAG AAGCATTAGT GTCTATGATT 
TTCCCTTTCC ACTGGCCGTG CCCATATGTT CCTCTCTGCC CACTGGCTTT AGCAGATGTC 
TTGAGTGCAC CATGTCCATT CATTGTAGGA ATTGATTCAA GATATTTTGA TCTCTATGAC 
CCTCCACCAG ATGTTAGTTG TGTTGACCTA GATACCAACA CTATTTCTCA AACTGGCGAT 
AAGAAAAATG TAGCCTGGAA GATACTTCCA AAGAAACCAT GTAAAAACCT GATGAACACA 
CTGAATAATT TGCACCAGCA GTTGGCAAAA TTGCAGCAGA GACCAAGAGA TGATGGACTA 
ATGGACTTAG CAATGAATGA CTATGATTTT AATTCTGGAA AGAGGTTGCA TATGATAGAT 
TTGGAAATCC AAGAGGCATT TTTGTGTTTC ATGGCCTCTA TTTTAAAAGG TTATAGATCA 
TACTTAAGAC CAATAACACA AGCCCCTTCT GAGACAGCCA CCGATGCATC CTCTCTTTTT 
GCCCTACAAG CTTTCTTAAG AAGCCGAGAC CGGTCACATC AAAAATTCTA TAACATGATG 
ACCAAAACAC AAATGTTCAT TCGCTTCATT GAGGAATGTT CTTTTGTCAG TGATAAAGAT 
GCAAGCCTGG CATTTTTTGA TGATTGTGTA GATAAAGTGG ATATGGACAA GAGTGGTGAA 
ATGCGACTTA TAGAACTGGA TGAATCTTTC AAAAGTGAAC ATACTGTTTT TGTAACACCA 
CCTGAGATAC CCCATCTACC CAATGGTGAA GAACCCCCTT TACAGTACAG CTATAATGGA 
TTTCCAGTTC TTAGAAACAA TTTATTTGAG AGACCTGAAG GATTTCTACA AACACGAAAG 
AACAAATTGC CTTCAAAATC AAGTAGTCCT AGTAGCCCTT CTCCCATGTT CAGAAGAACA 
AAACAGGAAA TTAAATCAGC CCATAAAATT GCCAAGAGGT ACTCTTCCAT TCCTCAGATG 
TGGTCTAGGT GTCTGCTGCG CCACTGCTAT GGACTGTGGT TCATTTGTCT GCCAGCTTAT 
GTGAAAGTGT GTCATTCAAA AGTCAGGGCA CTGAAGACAG CATATGATGT GCTCAAAAAA 
ATGCAGTCGA AAAAGATGGA TCCACCTGAT GAAGTGTGCT ACCGCATTCT CATGCAGCTC 
TGTGGGCAGT ACGATCAGCC CGTGCTTGCA GTTCGAGTGC TTTTTGAAAT GCAGAAAGCT 
GGTATTGATC CCAATGCCAT TACTTATGGT TATTATAATA AGGCTGTTTT GGAAAGCACC 
TGGCCCTCAA GAAGTCGTAG TGGCTATTTT CTTTGGACAA AAGTAAGAAA CGTTGTTTTA 
GGAGTAGCAC AGTTCAAAAG AGCTTTAAAG AAACAGGCAC ACTTATCACA AACAACTCTC 
TCAGCAGATG GCAGTGACCT GGATGCTGTT AGTCATGGCA GCATGGATAG TGGTCATGGG 
ACACACACTG TGGAGCAGGC ACCTTTTAAT ACGGGTTTAA TCAAAGTATA TGCTACTGAT 
GATAGATCTA GTACAGGTGG CCAGTCTGAT CTTGGATATA ATTCATTATC TAAGGATGAA 
GTTAGAAGAG GGGATATATC TACTGAGGAA ATTCAAGAAG AAAAAGATAA GAAAGGAAGT 
GATTCTAGTT CCCTGTCAGA GAGTGAGAGT ACAAAAGGAA GTGCTGACTG TCTTCCTAAA 
CTCAATTATC AAAGTTCTTC CAGTATAGTT CGCCTCAGTG GTACAAATAA CAATGGTGCT 
GATAAAATAT CAGGAGAAAG CACAGAAAGC ACATCTAAGC TGCTCTTACT ATCATCTTTT 
GAGGGTACAA ATGAAACAGG AAATAGTCAA AATAGATGCT TCAGGAAAAG ACATAAAAGT 
GACAACGAAC CTAATTTGCA ACAGCGACTC TCCTGGGGAA GCAGAAACCG CAATCTTAGT 
GGAGGAGTGC TGATGGGACT TATGCTTAAT AGAATCAACC AGGAAGCAAA CCCTGGAGAC 
ATGGTTGAAA AACTAGGAGC TGATGCAAAA ATTCTTTCAA ACGTTATATC ACAAAGCACA 
AGACCAACTA GTCTTGATAT TGGAAAACCC CCTTTAAGAT CAAAAAGAGA CAGTCTAGAA 
AAGGAATCTA GCGACGATGA TACACCTTTC GATGGTTCTA ATTGTTTGGC AGATAAAGTG 
GATTCTCCTG TTATTTTTGA TTTGGAAGAC TTAGACAGTG AGACAGATGG ATCAAAAGTG 
GGATACATTG CTTCACAAAA TCCCAGAAGG ATTCAGCGTA TGAGCAGCAG CTTCTCGGTA 
AAACCTTCTG AAAAAACGGA TGTTGCCACA GGATTTGATC CCCTCTCTCT TTTGGTTGCT 
GAGACTGAAC AGCAGCAAAA AGAGGAAGAG GAGGAGGATG ATGAAGATAG CAAAAGCGTT 
TCAACACCAT CCGCCAGGCG TGACTTAGCC GAAGAGATTG TGATGTATAT GAATAACATG 
AGCAGTCCTT TGACAAGCCG CACACCAAGC ATCGATTTAC AACGGGCATG TGATGACAAA 
TCAACCAGTA AGAAAAGTCC AACATTTGTC AAGGCATGCA GGAGATCTAG CCTGCCTCCT 
CATTCTCCGA GGCCAGTGAG ACTTACCAAA TCTAAAAGTT ATACAAAAAC TGAGGAGAGA 
CCTCGGGACA GACTCTGGTC TTCTCCAGCC TTCTCACCTA CTTGCCCATT TAGGGAAGAA 
TCTCAAGACG CATTAACCCA TTCATCACCT TCATTTAATT TAGATACACT GCTAGTACCT 
AAACTAGATG TTCTAAGGAA CAGTATGTTC ACTGCTGGGA AAGGAGTTGC CGAGAAAGCC 
AGCAAATGGT ATTCAAGATT CACCATGTAT ACCACATCAT CTAAGGATCA AAGTTCTGAT 
CGTACCAGTC TTTCTTCAGT GGGAGCCCAG GATTCTGAAT CTACCTCTTT AACAGATGAA 
GATGTTTGCC ATGAATTGGA AGGAGCCACT TCCTCCCAAG AGAGCTGTGC TGCTTCAGGG 
AATAAGGGAA TTGATTTCAG CAGAACAAGC CTGGAAAGTT CTGCCTCCTT AGAAGGATCC 
CTGTCAAAGT TTGCCTTACC TGGGAAATCA GAAGTGGCAT CTTCCTGCAA CACAAGTAAT 
ACAAATATCT TCCAGAACTA TGCAATGGAG GTTCTTATCT CAAGTTGCTC TCGGTGTAGA 
ACTTGTGATT GTCTTGTCCA TGATGAGGAA ATCATGGCTG GCTGGACAGC AGATGATTCA 
AATCTCAATA CTACATGCCC GTTTTGTGGC AATCTCTTCT TGCCCTTTCT GAATATTGAA 
ATAAGAGATT TAAGACGACC CGGAAGATAC TTTCTGAAGT CAAGTCCATC TACAGAAAAT 
ATGCATTTTC CATCCTCCCT TTCAAGTCAG ACAAGGCAGT CTTGTATCTC AACATCAGCC 
TCTGGTCTTG ACACGTCTGT TATCTCTGTT CAAGGGAATT TTGATCTCAA TAGCAAATCT 
AAACTGCTGG AAAATACATG TGCCCGAACT ATTCACATCC CTGCTGATAG ATCAAAAACA 
GGTGTGTCAA AATGTCCAAT ATTTCCAATG GCCAGGAGTG TTAGTACTTA TGGCCCCTTG 
GATAAGGAAG ACACTGGAGG CCAGAAGCTC ATTCCTACAG GCAGCCTGCC AACTACTTTA 
CAAGGAGCTA CAGATTCTTT AGGATTAGAG TGGCACCTTC CTAGTCCAGA TCCTATCACA 
GTTCCATATC TTAGTCCTTT AGTGGTATGG AAGGAGCTTG AAAGCTTACT GGAAAATGAA 
GGCGACCATG CAATAACAGT GGCAGACTTT GTGGATCATC ATCCAATCGT GTTTTGGAAC 
CTGGTGTGGT ATTTTAGACG TCTCGACTTA CCCAGTAACT TACCTGGATT GATTCTTTCT 
TCTGAGCACT GTAACAAGTA TTCAAAGATT CCTCGCCACT GTATGTCGGA AGATAGTAAA 
TACGTTTTAA TACAAATGTT ATGGGACAAT ATGAAATTAC ATCAGGATCC GGGACAGCCT 
TTGTACATCC TCTGGAATGC CCACAGTCAA AATCGTACTC TTTTGTTTGA GACCCAAAAG 
TATCCAATGG TCCATTTATT GCAAAAGGGT GATAATTCAT TTAACCAGGA ACTGTTGAAA 
AATATGGTAA AAAGCATTAA AATGAATGAT GTCTATGGAC CAATGAGTCA AATTTTAGAG 
ACACTGAATA AATGTCCTCA TTTTAAAAGA CAGAGGAGTT TATATAGAGA AATACTATTT 
CTCTCACTTG TGGCACTGGG AAGAGAAAAC ATTGATATAG ATGCATTTGA TAAAGAATAC 
AAGATGGCAT ACGATCGTCT CACACCTAGT CAAGTCAAGA GTACACACAA CTGTGATAGA 
CCACCAAGTA CAGGGGTGAT GGAATGTCGA AAAACCTTTG GAGAACCTTA TCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DENND4A ( XM_022412983.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022268691.1 Canis lupus familiaris DENN domain containing 4A (DENND4A), transcript variant X2, mRNA.

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CloneID	OCe29018
Clone ID Related Accession (Same CDS sequence)	XM_005638527.3 , XM_022412983.1 , XM_005638527.2 , XM_022412984.1
Accession Version	XM_022412983.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5757bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	C-myc promoter-binding protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006612.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTGAAG ATAAGGGTCC TCGTGTTGCT GACTACTTTG TTGTAGCAGG ATTAACTGAT 
GTTTCAAAGC CTCTTGAAGA AGAAATTCAC TTCAATGATG CCTGTCATAA AGTAGCTAAA 
CCAAAAGAAC CTATCACAGA TGTTTCGGTG ATTATTAAAT CTCTTGGGGA AGAAGTGCCA 
CGGGATTATA TATGTATTGA TGTTACCCCA ACTGGATTGT CGGCTGATCT CAATAATGGA 
AGTCTTGTGG GGCCACAGAT TTACCTTTGC TACAGAAGAG GAAGAGATAA GCCTCCACTT 
ACAGATCTGG GGGTTTTGTA TGACTGGAAA GAAAGATTGA AACAGGGATG TGAAATTATT 
CAGAGTACTC CCTATGGACA CCCTGCAAAT ATTAGTGGCA GTACCTCATC ACAAAAAATT 
TATATAACTT ACCGAAGAGC CTCTGAAAAC ATGACGCAAA ATACGTTGGC TGTCACAGAC 
ATATGTATTA TTATACCCAG TAAAGGAGAG AGTCCACCAC ACACGTTTTG CAAAGTCGAT 
AAGAATCTCA ATAACAGTAT GTGGGGATCT GCAGTGTACT TATGTTATAA AAAATCAGTG 
GCAAAGACTA ACACTATATC CTATAAAGCT GGTCTAATTT GTAGATATCC TCAAGAAGAT 
TACGAATCTT TCTCACTCCC AGAATCTGTT CCCCTATTTT GTTTACCAAT GGGTGCAACT 
ATTGAATGTT GGCCACCAAA TAGCAAATAC CCTCTCCCTG TATTTTCTAC ATTTGTGTTA 
ACTGGAGCCT CAGCTGAAAA GGTCTATGGT GCTGCTATTC AGTTTTATGA ACCATACTCT 
GAGGAGAATC TCACAGAAAA ACAGAGACTT CTTTTGGGTT TAGCATCATC AGCAGATGGG 
AAATCTGATA GTTCTAAAAC AATTCACACT AGTAAATGCA TCTGTCTTCT TTCTCACTGG 
CCTTTTTTTG ATGCATTCAG GAAATTTTTG ACCTTTCTGT ATCGTTACTC TATCTCTGGA 
CCTCATGTCC TTCCAATTGA GAAGCATATT TCTCATTTTA TGCATAAAGT TCCTTTTCCA 
TCTCCTCAGA GGCCACGGAT TTTAGTTCAG TTATCGCCAC ATGATAATCT GATTCTCAGT 
CAACCTGTTT CTTCACCTCT TCCACTAAGT GGTGGCAAAT TTTCAACACT ACTTCAAAAT 
TTAGGCCCTG AAAATGCTGT AACACTACTG GTGTTTGCAG TAACAGAGCA CAAGATTCTC 
ATCCATTCCT TACGGCCTTC CGTGCTTACT AGCGTGACAG AAGCATTAGT GTCTATGATT 
TTCCCTTTCC ACTGGCCGTG CCCATATGTT CCTCTCTGCC CACTGGCTTT AGCAGATGTC 
TTGAGTGCAC CATGTCCATT CATTGTAGGA ATTGATTCAA GATATTTTGA TCTCTATGAC 
CCTCCACCAG ATGTTAGTTG TGTTGACCTA GATACCAACA CTATTTCTCA AACTGGCGAT 
AAGAAAAATG TAGCCTGGAA GATACTTCCA AAGAAACCAT GTAAAAACCT GATGAACACA 
CTGAATAATT TGCACCAGCA GTTGGCAAAA TTGCAGCAGA GACCAAGAGA TGATGGACTA 
ATGGACTTAG CAATGAATGA CTATGATTTT AATTCTGGAA AGAGGTTGCA TATGATAGAT 
TTGGAAATCC AAGAGGCATT TTTGTGTTTC ATGGCCTCTA TTTTAAAAGG TTATAGATCA 
TACTTAAGAC CAATAACACA AGCCCCTTCT GAGACAGCCA CCGATGCATC CTCTCTTTTT 
GCCCTACAAG CTTTCTTAAG AAGCCGAGAC CGGTCACATC AAAAATTCTA TAACATGATG 
ACCAAAACAC AAATGTTCAT TCGCTTCATT GAGGAATGTT CTTTTGTCAG TGATAAAGAT 
GCAAGCCTGG CATTTTTTGA TGATTGTGTA GATAAAGTGG ATATGGACAA GAGTGGTGAA 
ATGCGACTTA TAGAACTGGA TGAATCTTTC AAAAGTGAAC ATACTGTTTT TGTAACACCA 
CCTGAGATAC CCCATCTACC CAATGGTGAA GAACCCCCTT TACAGTACAG CTATAATGGA 
TTTCCAGTTC TTAGAAACAA TTTATTTGAG AGACCTGAAG GATTTCTACA AACACGAAAG 
AACAAATTGC CTTCAAAATC AAGTAGTCCT AGTAGCCCTT CTCCCATGTT CAGAAGAACA 
AAACAGGAAA TTAAATCAGC CCATAAAATT GCCAAGAGGT ACTCTTCCAT TCCTCAGATG 
TGGTCTAGGT GTCTGCTGCG CCACTGCTAT GGACTGTGGT TCATTTGTCT GCCAGCTTAT 
GTGAAAGTGT GTCATTCAAA AGTCAGGGCA CTGAAGACAG CATATGATGT GCTCAAAAAA 
ATGCAGTCGA AAAAGATGGA TCCACCTGAT GAAGTGTGCT ACCGCATTCT CATGCAGCTC 
TGTGGGCAGT ACGATCAGCC CGTGCTTGCA GTTCGAGTGC TTTTTGAAAT GCAGAAAGCT 
GGTATTGATC CCAATGCCAT TACTTATGGT TATTATAATA AGGCTGTTTT GGAAAGCACC 
TGGCCCTCAA GAAGTCGTAG TGGCTATTTT CTTTGGACAA AAGTAAGAAA CGTTGTTTTA 
GGAGTAGCAC AGTTCAAAAG AGCTTTAAAG AAACAGGCAC ACTTATCACA AACAACTCTC 
TCAGCAGATG GCAGTGACCT GGATGCTGTT AGTCATGGCA GCATGGATAG TGGTCATGGG 
ACACACACTG TGGAGCAGGC ACCTTTTAAT ACGGGTTTAA TCAAAGTATA TGCTACTGAT 
GATAGATCTA GTACAGGTGG CCAGTCTGAT CTTGGATATA ATTCATTATC TAAGGATGAA 
GTTAGAAGAG GGGATATATC TACTGAGGAA ATTCAAGAAG AAAAAGATAA GAAAGGAAGT 
GATTCTAGTT CCCTGTCAGA GAGTGAGAGT ACAAAAGGAA GTGCTGACTG TCTTCCTAAA 
CTCAATTATC AAAGTTCTTC CAGTATAGTT CGCCTCAGTG GTACAAATAA CAATGGTGCT 
GATAAAATAT CAGGAGAAAG CACAGAAAGC ACATCTAAGC TGCTCTTACT ATCATCTTTT 
GAGGGTACAA ATGAAACAGG AAATAGTCAA AATAGATGCT TCAGGAAAAG ACATAAAAGT 
GACAACGAAC CTAATTTGCA ACAGCGACTC TCCTGGGGAA GCAGAAACCG CAATCTTAGT 
GGAGGAGTGC TGATGGGACT TATGCTTAAT AGAATCAACC AGGAAGCAAA CCCTGGAGAC 
ATGGTTGAAA AACTAGGAGC TGATGCAAAA ATTCTTTCAA ACGTTATATC ACAAAGCACA 
AGACCAACTA GTCTTGATAT TGGAAAACCC CCTTTAAGAT CAAAAAGAGA CAGTCTAGAA 
AAGGAATCTA GCGACGATGA TACACCTTTC GATGGTTCTA ATTGTTTGGC AGATAAAGTG 
GATTCTCCTG TTATTTTTGA TTTGGAAGAC TTAGACAGTG AGACAGATGG ATCAAAAGTG 
GGATACATTG CTTCACAAAA TCCCAGAAGG ATTCAGCGTA TGAGCAGCAG CTTCTCGGTA 
AAACCTTCTG AAAAAACGGA TGTTGCCACA GGATTTGATC CCCTCTCTCT TTTGGTTGCT 
GAGACTGAAC AGCAGCAAAA AGAGGAAGAG GAGGAGGATG ATGAAGATAG CAAAAGCGTT 
TCAACACCAT CCGCCAGGCG TGACTTAGCC GAAGAGATTG TGATGTATAT GAATAACATG 
AGCAGTCCTT TGACAAGCCG CACACCAAGC ATCGATTTAC AACGGGCATG TGATGACAAA 
TCAACCAGTA AGAAAAGTCC AACATTTGTC AAGGCATGCA GGAGATCTAG CCTGCCTCCT 
CATTCTCCGA GGCCAGTGAG ACTTACCAAA TCTAAAAGTT ATACAAAAAC TGAGGAGAGA 
CCTCGGGACA GACTCTGGTC TTCTCCAGCC TTCTCACCTA CTTGCCCATT TAGGGAAGAA 
TCTCAAGACG CATTAACCCA TTCATCACCT TCATTTAATT TAGATACACT GCTAGTACCT 
AAACTAGATG TTCTAAGGAA CAGTATGTTC ACTGCTGGGA AAGGAGTTGC CGAGAAAGCC 
AGCAAATGGT ATTCAAGATT CACCATGTAT ACCACATCAT CTAAGGATCA AAGTTCTGAT 
CGTACCAGTC TTTCTTCAGT GGGAGCCCAG GATTCTGAAT CTACCTCTTT AACAGATGAA 
GATGTTTGCC ATGAATTGGA AGGAGCCACT TCCTCCCAAG AGAGCTGTGC TGCTTCAGGG 
AATAAGGGAA TTGATTTCAG CAGAACAAGC CTGGAAAGTT CTGCCTCCTT AGAAGGATCC 
CTGTCAAAGT TTGCCTTACC TGGGAAATCA GAAGTGGCAT CTTCCTGCAA CACAAGTAAT 
ACAAATATCT TCCAGAACTA TGCAATGGAG GTTCTTATCT CAAGTTGCTC TCGGTGTAGA 
ACTTGTGATT GTCTTGTCCA TGATGAGGAA ATCATGGCTG GCTGGACAGC AGATGATTCA 
AATCTCAATA CTACATGCCC GTTTTGTGGC AATCTCTTCT TGCCCTTTCT GAATATTGAA 
ATAAGAGATT TAAGACGACC CGGAAGATAC TTTCTGAAGT CAAGTCCATC TACAGAAAAT 
ATGCATTTTC CATCCTCCCT TTCAAGTCAG ACAAGGCAGT CTTGTATCTC AACATCAGCC 
TCTGGTCTTG ACACGTCTGT TATCTCTGTT CAAGGGAATT TTGATCTCAA TAGCAAATCT 
AAACTGCTGG AAAATACATG TGCCCGAACT ATTCACATCC CTGCTGATAG ATCAAAAACA 
GGTGTGTCAA AATGTCCAAT ATTTCCAATG GCCAGGAGTG TTAGTACTTA TGGCCCCTTG 
GATAAGGAAG ACACTGGAGG CCAGAAGCTC ATTCCTACAG GCAGCCTGCC AACTACTTTA 
CAAGGAGCTA CAGATTCTTT AGGATTAGAG TGGCACCTTC CTAGTCCAGA TCCTATCACA 
GTTCCATATC TTAGTCCTTT AGTGGTATGG AAGGAGCTTG AAAGCTTACT GGAAAATGAA 
GGCGACCATG CAATAACAGT GGCAGACTTT GTGGATCATC ATCCAATCGT GTTTTGGAAC 
CTGGTGTGGT ATTTTAGACG TCTCGACTTA CCCAGTAACT TACCTGGATT GATTCTTTCT 
TCTGAGCACT GTAACAAGTA TTCAAAGATT CCTCGCCACT GTATGTCGGA AGATAGTAAA 
TACGTTTTAA TACAAATGTT ATGGGACAAT ATGAAATTAC ATCAGGATCC GGGACAGCCT 
TTGTACATCC TCTGGAATGC CCACAGTCAA AATCGTACTC TTTTGTTTGA GACCCAAAAG 
TATCCAATGG TCCATTTATT GCAAAAGGGT GATAATTCAT TTAACCAGGA ACTGTTGAAA 
AATATGGTAA AAAGCATTAA AATGAATGAT GTCTATGGAC CAATGAGTCA AATTTTAGAG 
ACACTGAATA AATGTCCTCA TTTTAAAAGA CAGAGGAGTT TATATAGAGA AATACTATTT 
CTCTCACTTG TGGCACTGGG AAGAGAAAAC ATTGATATAG ATGCATTTGA TAAAGAATAC 
AAGATGGCAT ACGATCGTCT CACACCTAGT CAAGTCAAGA GTACACACAA CTGTGATAGA 
CCACCAAGTA CAGGGGTGAT GGAATGTCGA AAAACCTTTG GAGAACCTTA TCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022268691.1
CDS	348..6104
Translation	MIEDKGPRVADYFVVAGLTDVSKPLEEEIHFNDACHKVAKPKEPITDVSVIIKSLGEEVPRDYICIDVTPTGLSADLNNGSLVGPQIYLCYRRGRDKPPLTDLGVLYDWKERLKQGCEIIQSTPYGHPANISGSTSSQKIYITYRRASENMTQNTLAVTDICIIIPSKGESPPHTFCKVDKNLNNSMWGSAVYLCYKKSVAKTNTISYKAGLICRYPQEDYESFSLPESVPLFCLPMGATIECWPPNSKYPLPVFSTFVLTGASAEKVYGAAIQFYEPYSEENLTEKQRLLLGLASSADGKSDSSKTIHTSKCICLLSHWPFFDAFRKFLTFLYRYSISGPHVLPIEKHISHFMHKVPFPSPQRPRILVQLSPHDNLILSQPVSSPLPLSGGKFSTLLQNLGPENAVTLLVFAVTEHKILIHSLRPSVLTSVTEALVSMIFPFHWPCPYVPLCPLALADVLSAPCPFIVGIDSRYFDLYDPPPDVSCVDLDTNTISQTGDKKNVAWKILPKKPCKNLMNTLNNLHQQLAKLQQRPRDDGLMDLAMNDYDFNSGKRLHMIDLEIQEAFLCFMASILKGYRSYLRPITQAPSETATDASSLFALQAFLRSRDRSHQKFYNMMTKTQMFIRFIEECSFVSDKDASLAFFDDCVDKVDMDKSGEMRLIELDESFKSEHTVFVTPPEIPHLPNGEEPPLQYSYNGFPVLRNNLFERPEGFLQTRKNKLPSKSSSPSSPSPMFRRTKQEIKSAHKIAKRYSSIPQMWSRCLLRHCYGLWFICLPAYVKVCHSKVRALKTAYDVLKKMQSKKMDPPDEVCYRILMQLCGQYDQPVLAVRVLFEMQKAGIDPNAITYGYYNKAVLESTWPSRSRSGYFLWTKVRNVVLGVAQFKRALKKQAHLSQTTLSADGSDLDAVSHGSMDSGHGTHTVEQAPFNTGLIKVYATDDRSSTGGQSDLGYNSLSKDEVRRGDISTEEIQEEKDKKGSDSSSLSESESTKGSADCLPKLNYQSSSSIVRLSGTNNNGADKISGESTESTSKLLLLSSFEGTNETGNSQNRCFRKRHKSDNEPNLQQRLSWGSRNRNLSGGVLMGLMLNRINQEANPGDMVEKLGADAKILSNVISQSTRPTSLDIGKPPLRSKRDSLEKESSDDDTPFDGSNCLADKVDSPVIFDLEDLDSETDGSKVGYIASQNPRRIQRMSSSFSVKPSEKTDVATGFDPLSLLVAETEQQQKEEEEEDDEDSKSVSTPSARRDLAEEIVMYMNNMSSPLTSRTPSIDLQRACDDKSTSKKSPTFVKACRRSSLPPHSPRPVRLTKSKSYTKTEERPRDRLWSSPAFSPTCPFREESQDALTHSSPSFNLDTLLVPKLDVLRNSMFTAGKGVAEKASKWYSRFTMYTTSSKDQSSDRTSLSSVGAQDSESTSLTDEDVCHELEGATSSQESCAASGNKGIDFSRTSLESSASLEGSLSKFALPGKSEVASSCNTSNTNIFQNYAMEVLISSCSRCRTCDCLVHDEEIMAGWTADDSNLNTTCPFCGNLFLPFLNIEIRDLRRPGRYFLKSSPSTENMHFPSSLSSQTRQSCISTSASGLDTSVISVQGNFDLNSKSKLLENTCARTIHIPADRSKTGVSKCPIFPMARSVSTYGPLDKEDTGGQKLIPTGSLPTTLQGATDSLGLEWHLPSPDPITVPYLSPLVVWKELESLLENEGDHAITVADFVDHHPIVFWNLVWYFRRLDLPSNLPGLILSSEHCNKYSKIPRHCMSEDSKYVLIQMLWDNMKLHQDPGQPLYILWNAHSQNRTLLFETQKYPMVHLLQKGDNSFNQELLKNMVKSIKMNDVYGPMSQILETLNKCPHFKRQRSLYREILFLSLVALGRENIDIDAFDKEYKMAYDRLTPSQVKSTHNCDRPPSTGVMECRKTFGEPYL

Target ORF information:

RefSeq Version	XM_022412983.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris DENN domain containing 4A (DENND4A), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022412983.1

ORF Insert Sequence:

ATGATTGAAG ATAAGGGTCC TCGTGTTGCT GACTACTTTG TTGTAGCAGG ATTAACTGAT 
GTTTCAAAGC CTCTTGAAGA AGAAATTCAC TTCAATGATG CCTGTCATAA AGTAGCTAAA 
CCAAAAGAAC CTATCACAGA TGTTTCGGTG ATTATTAAAT CTCTTGGGGA AGAAGTGCCA 
CGGGATTATA TATGTATTGA TGTTACCCCA ACTGGATTGT CGGCTGATCT CAATAATGGA 
AGTCTTGTGG GGCCACAGAT TTACCTTTGC TACAGAAGAG GAAGAGATAA GCCTCCACTT 
ACAGATCTGG GGGTTTTGTA TGACTGGAAA GAAAGATTGA AACAGGGATG TGAAATTATT 
CAGAGTACTC CCTATGGACA CCCTGCAAAT ATTAGTGGCA GTACCTCATC ACAAAAAATT 
TATATAACTT ACCGAAGAGC CTCTGAAAAC ATGACGCAAA ATACGTTGGC TGTCACAGAC 
ATATGTATTA TTATACCCAG TAAAGGAGAG AGTCCACCAC ACACGTTTTG CAAAGTCGAT 
AAGAATCTCA ATAACAGTAT GTGGGGATCT GCAGTGTACT TATGTTATAA AAAATCAGTG 
GCAAAGACTA ACACTATATC CTATAAAGCT GGTCTAATTT GTAGATATCC TCAAGAAGAT 
TACGAATCTT TCTCACTCCC AGAATCTGTT CCCCTATTTT GTTTACCAAT GGGTGCAACT 
ATTGAATGTT GGCCACCAAA TAGCAAATAC CCTCTCCCTG TATTTTCTAC ATTTGTGTTA 
ACTGGAGCCT CAGCTGAAAA GGTCTATGGT GCTGCTATTC AGTTTTATGA ACCATACTCT 
GAGGAGAATC TCACAGAAAA ACAGAGACTT CTTTTGGGTT TAGCATCATC AGCAGATGGG 
AAATCTGATA GTTCTAAAAC AATTCACACT AGTAAATGCA TCTGTCTTCT TTCTCACTGG 
CCTTTTTTTG ATGCATTCAG GAAATTTTTG ACCTTTCTGT ATCGTTACTC TATCTCTGGA 
CCTCATGTCC TTCCAATTGA GAAGCATATT TCTCATTTTA TGCATAAAGT TCCTTTTCCA 
TCTCCTCAGA GGCCACGGAT TTTAGTTCAG TTATCGCCAC ATGATAATCT GATTCTCAGT 
CAACCTGTTT CTTCACCTCT TCCACTAAGT GGTGGCAAAT TTTCAACACT ACTTCAAAAT 
TTAGGCCCTG AAAATGCTGT AACACTACTG GTGTTTGCAG TAACAGAGCA CAAGATTCTC 
ATCCATTCCT TACGGCCTTC CGTGCTTACT AGCGTGACAG AAGCATTAGT GTCTATGATT 
TTCCCTTTCC ACTGGCCGTG CCCATATGTT CCTCTCTGCC CACTGGCTTT AGCAGATGTC 
TTGAGTGCAC CATGTCCATT CATTGTAGGA ATTGATTCAA GATATTTTGA TCTCTATGAC 
CCTCCACCAG ATGTTAGTTG TGTTGACCTA GATACCAACA CTATTTCTCA AACTGGCGAT 
AAGAAAAATG TAGCCTGGAA GATACTTCCA AAGAAACCAT GTAAAAACCT GATGAACACA 
CTGAATAATT TGCACCAGCA GTTGGCAAAA TTGCAGCAGA GACCAAGAGA TGATGGACTA 
ATGGACTTAG CAATGAATGA CTATGATTTT AATTCTGGAA AGAGGTTGCA TATGATAGAT 
TTGGAAATCC AAGAGGCATT TTTGTGTTTC ATGGCCTCTA TTTTAAAAGG TTATAGATCA 
TACTTAAGAC CAATAACACA AGCCCCTTCT GAGACAGCCA CCGATGCATC CTCTCTTTTT 
GCCCTACAAG CTTTCTTAAG AAGCCGAGAC CGGTCACATC AAAAATTCTA TAACATGATG 
ACCAAAACAC AAATGTTCAT TCGCTTCATT GAGGAATGTT CTTTTGTCAG TGATAAAGAT 
GCAAGCCTGG CATTTTTTGA TGATTGTGTA GATAAAGTGG ATATGGACAA GAGTGGTGAA 
ATGCGACTTA TAGAACTGGA TGAATCTTTC AAAAGTGAAC ATACTGTTTT TGTAACACCA 
CCTGAGATAC CCCATCTACC CAATGGTGAA GAACCCCCTT TACAGTACAG CTATAATGGA 
TTTCCAGTTC TTAGAAACAA TTTATTTGAG AGACCTGAAG GATTTCTACA AACACGAAAG 
AACAAATTGC CTTCAAAATC AAGTAGTCCT AGTAGCCCTT CTCCCATGTT CAGAAGAACA 
AAACAGGAAA TTAAATCAGC CCATAAAATT GCCAAGAGGT ACTCTTCCAT TCCTCAGATG 
TGGTCTAGGT GTCTGCTGCG CCACTGCTAT GGACTGTGGT TCATTTGTCT GCCAGCTTAT 
GTGAAAGTGT GTCATTCAAA AGTCAGGGCA CTGAAGACAG CATATGATGT GCTCAAAAAA 
ATGCAGTCGA AAAAGATGGA TCCACCTGAT GAAGTGTGCT ACCGCATTCT CATGCAGCTC 
TGTGGGCAGT ACGATCAGCC CGTGCTTGCA GTTCGAGTGC TTTTTGAAAT GCAGAAAGCT 
GGTATTGATC CCAATGCCAT TACTTATGGT TATTATAATA AGGCTGTTTT GGAAAGCACC 
TGGCCCTCAA GAAGTCGTAG TGGCTATTTT CTTTGGACAA AAGTAAGAAA CGTTGTTTTA 
GGAGTAGCAC AGTTCAAAAG AGCTTTAAAG AAACAGGCAC ACTTATCACA AACAACTCTC 
TCAGCAGATG GCAGTGACCT GGATGCTGTT AGTCATGGCA GCATGGATAG TGGTCATGGG 
ACACACACTG TGGAGCAGGC ACCTTTTAAT ACGGGTTTAA TCAAAGTATA TGCTACTGAT 
GATAGATCTA GTACAGGTGG CCAGTCTGAT CTTGGATATA ATTCATTATC TAAGGATGAA 
GTTAGAAGAG GGGATATATC TACTGAGGAA ATTCAAGAAG AAAAAGATAA GAAAGGAAGT 
GATTCTAGTT CCCTGTCAGA GAGTGAGAGT ACAAAAGGAA GTGCTGACTG TCTTCCTAAA 
CTCAATTATC AAAGTTCTTC CAGTATAGTT CGCCTCAGTG GTACAAATAA CAATGGTGCT 
GATAAAATAT CAGGAGAAAG CACAGAAAGC ACATCTAAGC TGCTCTTACT ATCATCTTTT 
GAGGGTACAA ATGAAACAGG AAATAGTCAA AATAGATGCT TCAGGAAAAG ACATAAAAGT 
GACAACGAAC CTAATTTGCA ACAGCGACTC TCCTGGGGAA GCAGAAACCG CAATCTTAGT 
GGAGGAGTGC TGATGGGACT TATGCTTAAT AGAATCAACC AGGAAGCAAA CCCTGGAGAC 
ATGGTTGAAA AACTAGGAGC TGATGCAAAA ATTCTTTCAA ACGTTATATC ACAAAGCACA 
AGACCAACTA GTCTTGATAT TGGAAAACCC CCTTTAAGAT CAAAAAGAGA CAGTCTAGAA 
AAGGAATCTA GCGACGATGA TACACCTTTC GATGGTTCTA ATTGTTTGGC AGATAAAGTG 
GATTCTCCTG TTATTTTTGA TTTGGAAGAC TTAGACAGTG AGACAGATGG ATCAAAAGTG 
GGATACATTG CTTCACAAAA TCCCAGAAGG ATTCAGCGTA TGAGCAGCAG CTTCTCGGTA 
AAACCTTCTG AAAAAACGGA TGTTGCCACA GGATTTGATC CCCTCTCTCT TTTGGTTGCT 
GAGACTGAAC AGCAGCAAAA AGAGGAAGAG GAGGAGGATG ATGAAGATAG CAAAAGCGTT 
TCAACACCAT CCGCCAGGCG TGACTTAGCC GAAGAGATTG TGATGTATAT GAATAACATG 
AGCAGTCCTT TGACAAGCCG CACACCAAGC ATCGATTTAC AACGGGCATG TGATGACAAA 
TCAACCAGTA AGAAAAGTCC AACATTTGTC AAGGCATGCA GGAGATCTAG CCTGCCTCCT 
CATTCTCCGA GGCCAGTGAG ACTTACCAAA TCTAAAAGTT ATACAAAAAC TGAGGAGAGA 
CCTCGGGACA GACTCTGGTC TTCTCCAGCC TTCTCACCTA CTTGCCCATT TAGGGAAGAA 
TCTCAAGACG CATTAACCCA TTCATCACCT TCATTTAATT TAGATACACT GCTAGTACCT 
AAACTAGATG TTCTAAGGAA CAGTATGTTC ACTGCTGGGA AAGGAGTTGC CGAGAAAGCC 
AGCAAATGGT ATTCAAGATT CACCATGTAT ACCACATCAT CTAAGGATCA AAGTTCTGAT 
CGTACCAGTC TTTCTTCAGT GGGAGCCCAG GATTCTGAAT CTACCTCTTT AACAGATGAA 
GATGTTTGCC ATGAATTGGA AGGAGCCACT TCCTCCCAAG AGAGCTGTGC TGCTTCAGGG 
AATAAGGGAA TTGATTTCAG CAGAACAAGC CTGGAAAGTT CTGCCTCCTT AGAAGGATCC 
CTGTCAAAGT TTGCCTTACC TGGGAAATCA GAAGTGGCAT CTTCCTGCAA CACAAGTAAT 
ACAAATATCT TCCAGAACTA TGCAATGGAG GTTCTTATCT CAAGTTGCTC TCGGTGTAGA 
ACTTGTGATT GTCTTGTCCA TGATGAGGAA ATCATGGCTG GCTGGACAGC AGATGATTCA 
AATCTCAATA CTACATGCCC GTTTTGTGGC AATCTCTTCT TGCCCTTTCT GAATATTGAA 
ATAAGAGATT TAAGACGACC CGGAAGATAC TTTCTGAAGT CAAGTCCATC TACAGAAAAT 
ATGCATTTTC CATCCTCCCT TTCAAGTCAG ACAAGGCAGT CTTGTATCTC AACATCAGCC 
TCTGGTCTTG ACACGTCTGT TATCTCTGTT CAAGGGAATT TTGATCTCAA TAGCAAATCT 
AAACTGCTGG AAAATACATG TGCCCGAACT ATTCACATCC CTGCTGATAG ATCAAAAACA 
GGTGTGTCAA AATGTCCAAT ATTTCCAATG GCCAGGAGTG TTAGTACTTA TGGCCCCTTG 
GATAAGGAAG ACACTGGAGG CCAGAAGCTC ATTCCTACAG GCAGCCTGCC AACTACTTTA 
CAAGGAGCTA CAGATTCTTT AGGATTAGAG TGGCACCTTC CTAGTCCAGA TCCTATCACA 
GTTCCATATC TTAGTCCTTT AGTGGTATGG AAGGAGCTTG AAAGCTTACT GGAAAATGAA 
GGCGACCATG CAATAACAGT GGCAGACTTT GTGGATCATC ATCCAATCGT GTTTTGGAAC 
CTGGTGTGGT ATTTTAGACG TCTCGACTTA CCCAGTAACT TACCTGGATT GATTCTTTCT 
TCTGAGCACT GTAACAAGTA TTCAAAGATT CCTCGCCACT GTATGTCGGA AGATAGTAAA 
TACGTTTTAA TACAAATGTT ATGGGACAAT ATGAAATTAC ATCAGGATCC GGGACAGCCT 
TTGTACATCC TCTGGAATGC CCACAGTCAA AATCGTACTC TTTTGTTTGA GACCCAAAAG 
TATCCAATGG TCCATTTATT GCAAAAGGGT GATAATTCAT TTAACCAGGA ACTGTTGAAA 
AATATGGTAA AAAGCATTAA AATGAATGAT GTCTATGGAC CAATGAGTCA AATTTTAGAG 
ACACTGAATA AATGTCCTCA TTTTAAAAGA CAGAGGAGTT TATATAGAGA AATACTATTT 
CTCTCACTTG TGGCACTGGG AAGAGAAAAC ATTGATATAG ATGCATTTGA TAAAGAATAC 
AAGATGGCAT ACGATCGTCT CACACCTAGT CAAGTCAAGA GTACACACAA CTGTGATAGA 
CCACCAAGTA CAGGGGTGAT GGAATGTCGA AAAACCTTTG GAGAACCTTA TCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DENND4A ( XM_005638527.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005638584.1 Canis lupus familiaris DENN/MADD domain containing 4A (DENND4A), transcript variant X1, mRNA.

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CloneID	OCe29018
Clone ID Related Accession (Same CDS sequence)	XM_005638527.3 , XM_022412983.1 , XM_005638527.2 , XM_022412984.1
Accession Version	XM_005638527.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5757bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	C-myc promoter-binding protein isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726113.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005638527.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTGAAG ATAAGGGTCC TCGTGTTGCT GACTACTTTG TTGTAGCAGG ATTAACTGAT 
GTTTCAAAGC CTCTTGAAGA AGAAATTCAC TTCAATGATG CCTGTCATAA AGTAGCTAAA 
CCAAAAGAAC CTATCACAGA TGTTTCGGTG ATTATTAAAT CTCTTGGGGA AGAAGTGCCA 
CGGGATTATA TATGTATTGA TGTTACCCCA ACTGGATTGT CGGCTGATCT CAATAATGGA 
AGTCTTGTGG GGCCACAGAT TTACCTTTGC TACAGAAGAG GAAGAGATAA GCCTCCACTT 
ACAGATCTGG GGGTTTTGTA TGACTGGAAA GAAAGATTGA AACAGGGATG TGAAATTATT 
CAGAGTACTC CCTATGGACA CCCTGCAAAT ATTAGTGGCA GTACCTCATC ACAAAAAATT 
TATATAACTT ACCGAAGAGC CTCTGAAAAC ATGACGCAAA ATACGTTGGC TGTCACAGAC 
ATATGTATTA TTATACCCAG TAAAGGAGAG AGTCCACCAC ACACGTTTTG CAAAGTCGAT 
AAGAATCTCA ATAACAGTAT GTGGGGATCT GCAGTGTACT TATGTTATAA AAAATCAGTG 
GCAAAGACTA ACACTATATC CTATAAAGCT GGTCTAATTT GTAGATATCC TCAAGAAGAT 
TACGAATCTT TCTCACTCCC AGAATCTGTT CCCCTATTTT GTTTACCAAT GGGTGCAACT 
ATTGAATGTT GGCCACCAAA TAGCAAATAC CCTCTCCCTG TATTTTCTAC ATTTGTGTTA 
ACTGGAGCCT CAGCTGAAAA GGTCTATGGT GCTGCTATTC AGTTTTATGA ACCATACTCT 
GAGGAGAATC TCACAGAAAA ACAGAGACTT CTTTTGGGTT TAGCATCATC AGCAGATGGG 
AAATCTGATA GTTCTAAAAC AATTCACACT AGTAAATGCA TCTGTCTTCT TTCTCACTGG 
CCTTTTTTTG ATGCATTCAG GAAATTTTTG ACCTTTCTGT ATCGTTACTC TATCTCTGGA 
CCTCATGTCC TTCCAATTGA GAAGCATATT TCTCATTTTA TGCATAAAGT TCCTTTTCCA 
TCTCCTCAGA GGCCACGGAT TTTAGTTCAG TTATCGCCAC ATGATAATCT GATTCTCAGT 
CAACCTGTTT CTTCACCTCT TCCACTAAGT GGTGGCAAAT TTTCAACACT ACTTCAAAAT 
TTAGGCCCTG AAAATGCTGT AACACTACTG GTGTTTGCAG TAACAGAGCA CAAGATTCTC 
ATCCATTCCT TACGGCCTTC CGTGCTTACT AGCGTGACAG AAGCATTAGT GTCTATGATT 
TTCCCTTTCC ACTGGCCGTG CCCATATGTT CCTCTCTGCC CACTGGCTTT AGCAGATGTC 
TTGAGTGCAC CATGTCCATT CATTGTAGGA ATTGATTCAA GATATTTTGA TCTCTATGAC 
CCTCCACCAG ATGTTAGTTG TGTTGACCTA GATACCAACA CTATTTCTCA AACTGGCGAT 
AAGAAAAATG TAGCCTGGAA GATACTTCCA AAGAAACCAT GTAAAAACCT GATGAACACA 
CTGAATAATT TGCACCAGCA GTTGGCAAAA TTGCAGCAGA GACCAAGAGA TGATGGACTA 
ATGGACTTAG CAATGAATGA CTATGATTTT AATTCTGGAA AGAGGTTGCA TATGATAGAT 
TTGGAAATCC AAGAGGCATT TTTGTGTTTC ATGGCCTCTA TTTTAAAAGG TTATAGATCA 
TACTTAAGAC CAATAACACA AGCCCCTTCT GAGACAGCCA CCGATGCATC CTCTCTTTTT 
GCCCTACAAG CTTTCTTAAG AAGCCGAGAC CGGTCACATC AAAAATTCTA TAACATGATG 
ACCAAAACAC AAATGTTCAT TCGCTTCATT GAGGAATGTT CTTTTGTCAG TGATAAAGAT 
GCAAGCCTGG CATTTTTTGA TGATTGTGTA GATAAAGTGG ATATGGACAA GAGTGGTGAA 
ATGCGACTTA TAGAACTGGA TGAATCTTTC AAAAGTGAAC ATACTGTTTT TGTAACACCA 
CCTGAGATAC CCCATCTACC CAATGGTGAA GAACCCCCTT TACAGTACAG CTATAATGGA 
TTTCCAGTTC TTAGAAACAA TTTATTTGAG AGACCTGAAG GATTTCTACA AACACGAAAG 
AACAAATTGC CTTCAAAATC AAGTAGTCCT AGTAGCCCTT CTCCCATGTT CAGAAGAACA 
AAACAGGAAA TTAAATCAGC CCATAAAATT GCCAAGAGGT ACTCTTCCAT TCCTCAGATG 
TGGTCTAGGT GTCTGCTGCG CCACTGCTAT GGACTGTGGT TCATTTGTCT GCCAGCTTAT 
GTGAAAGTGT GTCATTCAAA AGTCAGGGCA CTGAAGACAG CATATGATGT GCTCAAAAAA 
ATGCAGTCGA AAAAGATGGA TCCACCTGAT GAAGTGTGCT ACCGCATTCT CATGCAGCTC 
TGTGGGCAGT ACGATCAGCC CGTGCTTGCA GTTCGAGTGC TTTTTGAAAT GCAGAAAGCT 
GGTATTGATC CCAATGCCAT TACTTATGGT TATTATAATA AGGCTGTTTT GGAAAGCACC 
TGGCCCTCAA GAAGTCGTAG TGGCTATTTT CTTTGGACAA AAGTAAGAAA CGTTGTTTTA 
GGAGTAGCAC AGTTCAAAAG AGCTTTAAAG AAACAGGCAC ACTTATCACA AACAACTCTC 
TCAGCAGATG GCAGTGACCT GGATGCTGTT AGTCATGGCA GCATGGATAG TGGTCATGGG 
ACACACACTG TGGAGCAGGC ACCTTTTAAT ACGGGTTTAA TCAAAGTATA TGCTACTGAT 
GATAGATCTA GTACAGGTGG CCAGTCTGAT CTTGGATATA ATTCATTATC TAAGGATGAA 
GTTAGAAGAG GGGATATATC TACTGAGGAA ATTCAAGAAG AAAAAGATAA GAAAGGAAGT 
GATTCTAGTT CCCTGTCAGA GAGTGAGAGT ACAAAAGGAA GTGCTGACTG TCTTCCTAAA 
CTCAATTATC AAAGTTCTTC CAGTATAGTT CGCCTCAGTG GTACAAATAA CAATGGTGCT 
GATAAAATAT CAGGAGAAAG CACAGAAAGC ACATCTAAGC TGCTCTTACT ATCATCTTTT 
GAGGGTACAA ATGAAACAGG AAATAGTCAA AATAGATGCT TCAGGAAAAG ACATAAAAGT 
GACAACGAAC CTAATTTGCA ACAGCGACTC TCCTGGGGAA GCAGAAACCG CAATCTTAGT 
GGAGGAGTGC TGATGGGACT TATGCTTAAT AGAATCAACC AGGAAGCAAA CCCTGGAGAC 
ATGGTTGAAA AACTAGGAGC TGATGCAAAA ATTCTTTCAA ACGTTATATC ACAAAGCACA 
AGACCAACTA GTCTTGATAT TGGAAAACCC CCTTTAAGAT CAAAAAGAGA CAGTCTAGAA 
AAGGAATCTA GCGACGATGA TACACCTTTC GATGGTTCTA ATTGTTTGGC AGATAAAGTG 
GATTCTCCTG TTATTTTTGA TTTGGAAGAC TTAGACAGTG AGACAGATGG ATCAAAAGTG 
GGATACATTG CTTCACAAAA TCCCAGAAGG ATTCAGCGTA TGAGCAGCAG CTTCTCGGTA 
AAACCTTCTG AAAAAACGGA TGTTGCCACA GGATTTGATC CCCTCTCTCT TTTGGTTGCT 
GAGACTGAAC AGCAGCAAAA AGAGGAAGAG GAGGAGGATG ATGAAGATAG CAAAAGCGTT 
TCAACACCAT CCGCCAGGCG TGACTTAGCC GAAGAGATTG TGATGTATAT GAATAACATG 
AGCAGTCCTT TGACAAGCCG CACACCAAGC ATCGATTTAC AACGGGCATG TGATGACAAA 
TCAACCAGTA AGAAAAGTCC AACATTTGTC AAGGCATGCA GGAGATCTAG CCTGCCTCCT 
CATTCTCCGA GGCCAGTGAG ACTTACCAAA TCTAAAAGTT ATACAAAAAC TGAGGAGAGA 
CCTCGGGACA GACTCTGGTC TTCTCCAGCC TTCTCACCTA CTTGCCCATT TAGGGAAGAA 
TCTCAAGACG CATTAACCCA TTCATCACCT TCATTTAATT TAGATACACT GCTAGTACCT 
AAACTAGATG TTCTAAGGAA CAGTATGTTC ACTGCTGGGA AAGGAGTTGC CGAGAAAGCC 
AGCAAATGGT ATTCAAGATT CACCATGTAT ACCACATCAT CTAAGGATCA AAGTTCTGAT 
CGTACCAGTC TTTCTTCAGT GGGAGCCCAG GATTCTGAAT CTACCTCTTT AACAGATGAA 
GATGTTTGCC ATGAATTGGA AGGAGCCACT TCCTCCCAAG AGAGCTGTGC TGCTTCAGGG 
AATAAGGGAA TTGATTTCAG CAGAACAAGC CTGGAAAGTT CTGCCTCCTT AGAAGGATCC 
CTGTCAAAGT TTGCCTTACC TGGGAAATCA GAAGTGGCAT CTTCCTGCAA CACAAGTAAT 
ACAAATATCT TCCAGAACTA TGCAATGGAG GTTCTTATCT CAAGTTGCTC TCGGTGTAGA 
ACTTGTGATT GTCTTGTCCA TGATGAGGAA ATCATGGCTG GCTGGACAGC AGATGATTCA 
AATCTCAATA CTACATGCCC GTTTTGTGGC AATCTCTTCT TGCCCTTTCT GAATATTGAA 
ATAAGAGATT TAAGACGACC CGGAAGATAC TTTCTGAAGT CAAGTCCATC TACAGAAAAT 
ATGCATTTTC CATCCTCCCT TTCAAGTCAG ACAAGGCAGT CTTGTATCTC AACATCAGCC 
TCTGGTCTTG ACACGTCTGT TATCTCTGTT CAAGGGAATT TTGATCTCAA TAGCAAATCT 
AAACTGCTGG AAAATACATG TGCCCGAACT ATTCACATCC CTGCTGATAG ATCAAAAACA 
GGTGTGTCAA AATGTCCAAT ATTTCCAATG GCCAGGAGTG TTAGTACTTA TGGCCCCTTG 
GATAAGGAAG ACACTGGAGG CCAGAAGCTC ATTCCTACAG GCAGCCTGCC AACTACTTTA 
CAAGGAGCTA CAGATTCTTT AGGATTAGAG TGGCACCTTC CTAGTCCAGA TCCTATCACA 
GTTCCATATC TTAGTCCTTT AGTGGTATGG AAGGAGCTTG AAAGCTTACT GGAAAATGAA 
GGCGACCATG CAATAACAGT GGCAGACTTT GTGGATCATC ATCCAATCGT GTTTTGGAAC 
CTGGTGTGGT ATTTTAGACG TCTCGACTTA CCCAGTAACT TACCTGGATT GATTCTTTCT 
TCTGAGCACT GTAACAAGTA TTCAAAGATT CCTCGCCACT GTATGTCGGA AGATAGTAAA 
TACGTTTTAA TACAAATGTT ATGGGACAAT ATGAAATTAC ATCAGGATCC GGGACAGCCT 
TTGTACATCC TCTGGAATGC CCACAGTCAA AATCGTACTC TTTTGTTTGA GACCCAAAAG 
TATCCAATGG TCCATTTATT GCAAAAGGGT GATAATTCAT TTAACCAGGA ACTGTTGAAA 
AATATGGTAA AAAGCATTAA AATGAATGAT GTCTATGGAC CAATGAGTCA AATTTTAGAG 
ACACTGAATA AATGTCCTCA TTTTAAAAGA CAGAGGAGTT TATATAGAGA AATACTATTT 
CTCTCACTTG TGGCACTGGG AAGAGAAAAC ATTGATATAG ATGCATTTGA TAAAGAATAC 
AAGATGGCAT ACGATCGTCT CACACCTAGT CAAGTCAAGA GTACACACAA CTGTGATAGA 
CCACCAAGTA CAGGGGTGAT GGAATGTCGA AAAACCTTTG GAGAACCTTA TCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005638584.1
CDS	378..6134
Translation	MIEDKGPRVADYFVVAGLTDVSKPLEEEIHFNDACHKVAKPKEPITDVSVIIKSLGEEVPRDYICIDVTPTGLSADLNNGSLVGPQIYLCYRRGRDKPPLTDLGVLYDWKERLKQGCEIIQSTPYGHPANISGSTSSQKIYITYRRASENMTQNTLAVTDICIIIPSKGESPPHTFCKVDKNLNNSMWGSAVYLCYKKSVAKTNTISYKAGLICRYPQEDYESFSLPESVPLFCLPMGATIECWPPNSKYPLPVFSTFVLTGASAEKVYGAAIQFYEPYSEENLTEKQRLLLGLASSADGKSDSSKTIHTSKCICLLSHWPFFDAFRKFLTFLYRYSISGPHVLPIEKHISHFMHKVPFPSPQRPRILVQLSPHDNLILSQPVSSPLPLSGGKFSTLLQNLGPENAVTLLVFAVTEHKILIHSLRPSVLTSVTEALVSMIFPFHWPCPYVPLCPLALADVLSAPCPFIVGIDSRYFDLYDPPPDVSCVDLDTNTISQTGDKKNVAWKILPKKPCKNLMNTLNNLHQQLAKLQQRPRDDGLMDLAMNDYDFNSGKRLHMIDLEIQEAFLCFMASILKGYRSYLRPITQAPSETATDASSLFALQAFLRSRDRSHQKFYNMMTKTQMFIRFIEECSFVSDKDASLAFFDDCVDKVDMDKSGEMRLIELDESFKSEHTVFVTPPEIPHLPNGEEPPLQYSYNGFPVLRNNLFERPEGFLQTRKNKLPSKSSSPSSPSPMFRRTKQEIKSAHKIAKRYSSIPQMWSRCLLRHCYGLWFICLPAYVKVCHSKVRALKTAYDVLKKMQSKKMDPPDEVCYRILMQLCGQYDQPVLAVRVLFEMQKAGIDPNAITYGYYNKAVLESTWPSRSRSGYFLWTKVRNVVLGVAQFKRALKKQAHLSQTTLSADGSDLDAVSHGSMDSGHGTHTVEQAPFNTGLIKVYATDDRSSTGGQSDLGYNSLSKDEVRRGDISTEEIQEEKDKKGSDSSSLSESESTKGSADCLPKLNYQSSSSIVRLSGTNNNGADKISGESTESTSKLLLLSSFEGTNETGNSQNRCFRKRHKSDNEPNLQQRLSWGSRNRNLSGGVLMGLMLNRINQEANPGDMVEKLGADAKILSNVISQSTRPTSLDIGKPPLRSKRDSLEKESSDDDTPFDGSNCLADKVDSPVIFDLEDLDSETDGSKVGYIASQNPRRIQRMSSSFSVKPSEKTDVATGFDPLSLLVAETEQQQKEEEEEDDEDSKSVSTPSARRDLAEEIVMYMNNMSSPLTSRTPSIDLQRACDDKSTSKKSPTFVKACRRSSLPPHSPRPVRLTKSKSYTKTEERPRDRLWSSPAFSPTCPFREESQDALTHSSPSFNLDTLLVPKLDVLRNSMFTAGKGVAEKASKWYSRFTMYTTSSKDQSSDRTSLSSVGAQDSESTSLTDEDVCHELEGATSSQESCAASGNKGIDFSRTSLESSASLEGSLSKFALPGKSEVASSCNTSNTNIFQNYAMEVLISSCSRCRTCDCLVHDEEIMAGWTADDSNLNTTCPFCGNLFLPFLNIEIRDLRRPGRYFLKSSPSTENMHFPSSLSSQTRQSCISTSASGLDTSVISVQGNFDLNSKSKLLENTCARTIHIPADRSKTGVSKCPIFPMARSVSTYGPLDKEDTGGQKLIPTGSLPTTLQGATDSLGLEWHLPSPDPITVPYLSPLVVWKELESLLENEGDHAITVADFVDHHPIVFWNLVWYFRRLDLPSNLPGLILSSEHCNKYSKIPRHCMSEDSKYVLIQMLWDNMKLHQDPGQPLYILWNAHSQNRTLLFETQKYPMVHLLQKGDNSFNQELLKNMVKSIKMNDVYGPMSQILETLNKCPHFKRQRSLYREILFLSLVALGRENIDIDAFDKEYKMAYDRLTPSQVKSTHNCDRPPSTGVMECRKTFGEPYL

Target ORF information:

RefSeq Version	XM_005638527.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris DENN/MADD domain containing 4A (DENND4A), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005638527.2

ORF Insert Sequence:

ATGATTGAAG ATAAGGGTCC TCGTGTTGCT GACTACTTTG TTGTAGCAGG ATTAACTGAT 
GTTTCAAAGC CTCTTGAAGA AGAAATTCAC TTCAATGATG CCTGTCATAA AGTAGCTAAA 
CCAAAAGAAC CTATCACAGA TGTTTCGGTG ATTATTAAAT CTCTTGGGGA AGAAGTGCCA 
CGGGATTATA TATGTATTGA TGTTACCCCA ACTGGATTGT CGGCTGATCT CAATAATGGA 
AGTCTTGTGG GGCCACAGAT TTACCTTTGC TACAGAAGAG GAAGAGATAA GCCTCCACTT 
ACAGATCTGG GGGTTTTGTA TGACTGGAAA GAAAGATTGA AACAGGGATG TGAAATTATT 
CAGAGTACTC CCTATGGACA CCCTGCAAAT ATTAGTGGCA GTACCTCATC ACAAAAAATT 
TATATAACTT ACCGAAGAGC CTCTGAAAAC ATGACGCAAA ATACGTTGGC TGTCACAGAC 
ATATGTATTA TTATACCCAG TAAAGGAGAG AGTCCACCAC ACACGTTTTG CAAAGTCGAT 
AAGAATCTCA ATAACAGTAT GTGGGGATCT GCAGTGTACT TATGTTATAA AAAATCAGTG 
GCAAAGACTA ACACTATATC CTATAAAGCT GGTCTAATTT GTAGATATCC TCAAGAAGAT 
TACGAATCTT TCTCACTCCC AGAATCTGTT CCCCTATTTT GTTTACCAAT GGGTGCAACT 
ATTGAATGTT GGCCACCAAA TAGCAAATAC CCTCTCCCTG TATTTTCTAC ATTTGTGTTA 
ACTGGAGCCT CAGCTGAAAA GGTCTATGGT GCTGCTATTC AGTTTTATGA ACCATACTCT 
GAGGAGAATC TCACAGAAAA ACAGAGACTT CTTTTGGGTT TAGCATCATC AGCAGATGGG 
AAATCTGATA GTTCTAAAAC AATTCACACT AGTAAATGCA TCTGTCTTCT TTCTCACTGG 
CCTTTTTTTG ATGCATTCAG GAAATTTTTG ACCTTTCTGT ATCGTTACTC TATCTCTGGA 
CCTCATGTCC TTCCAATTGA GAAGCATATT TCTCATTTTA TGCATAAAGT TCCTTTTCCA 
TCTCCTCAGA GGCCACGGAT TTTAGTTCAG TTATCGCCAC ATGATAATCT GATTCTCAGT 
CAACCTGTTT CTTCACCTCT TCCACTAAGT GGTGGCAAAT TTTCAACACT ACTTCAAAAT 
TTAGGCCCTG AAAATGCTGT AACACTACTG GTGTTTGCAG TAACAGAGCA CAAGATTCTC 
ATCCATTCCT TACGGCCTTC CGTGCTTACT AGCGTGACAG AAGCATTAGT GTCTATGATT 
TTCCCTTTCC ACTGGCCGTG CCCATATGTT CCTCTCTGCC CACTGGCTTT AGCAGATGTC 
TTGAGTGCAC CATGTCCATT CATTGTAGGA ATTGATTCAA GATATTTTGA TCTCTATGAC 
CCTCCACCAG ATGTTAGTTG TGTTGACCTA GATACCAACA CTATTTCTCA AACTGGCGAT 
AAGAAAAATG TAGCCTGGAA GATACTTCCA AAGAAACCAT GTAAAAACCT GATGAACACA 
CTGAATAATT TGCACCAGCA GTTGGCAAAA TTGCAGCAGA GACCAAGAGA TGATGGACTA 
ATGGACTTAG CAATGAATGA CTATGATTTT AATTCTGGAA AGAGGTTGCA TATGATAGAT 
TTGGAAATCC AAGAGGCATT TTTGTGTTTC ATGGCCTCTA TTTTAAAAGG TTATAGATCA 
TACTTAAGAC CAATAACACA AGCCCCTTCT GAGACAGCCA CCGATGCATC CTCTCTTTTT 
GCCCTACAAG CTTTCTTAAG AAGCCGAGAC CGGTCACATC AAAAATTCTA TAACATGATG 
ACCAAAACAC AAATGTTCAT TCGCTTCATT GAGGAATGTT CTTTTGTCAG TGATAAAGAT 
GCAAGCCTGG CATTTTTTGA TGATTGTGTA GATAAAGTGG ATATGGACAA GAGTGGTGAA 
ATGCGACTTA TAGAACTGGA TGAATCTTTC AAAAGTGAAC ATACTGTTTT TGTAACACCA 
CCTGAGATAC CCCATCTACC CAATGGTGAA GAACCCCCTT TACAGTACAG CTATAATGGA 
TTTCCAGTTC TTAGAAACAA TTTATTTGAG AGACCTGAAG GATTTCTACA AACACGAAAG 
AACAAATTGC CTTCAAAATC AAGTAGTCCT AGTAGCCCTT CTCCCATGTT CAGAAGAACA 
AAACAGGAAA TTAAATCAGC CCATAAAATT GCCAAGAGGT ACTCTTCCAT TCCTCAGATG 
TGGTCTAGGT GTCTGCTGCG CCACTGCTAT GGACTGTGGT TCATTTGTCT GCCAGCTTAT 
GTGAAAGTGT GTCATTCAAA AGTCAGGGCA CTGAAGACAG CATATGATGT GCTCAAAAAA 
ATGCAGTCGA AAAAGATGGA TCCACCTGAT GAAGTGTGCT ACCGCATTCT CATGCAGCTC 
TGTGGGCAGT ACGATCAGCC CGTGCTTGCA GTTCGAGTGC TTTTTGAAAT GCAGAAAGCT 
GGTATTGATC CCAATGCCAT TACTTATGGT TATTATAATA AGGCTGTTTT GGAAAGCACC 
TGGCCCTCAA GAAGTCGTAG TGGCTATTTT CTTTGGACAA AAGTAAGAAA CGTTGTTTTA 
GGAGTAGCAC AGTTCAAAAG AGCTTTAAAG AAACAGGCAC ACTTATCACA AACAACTCTC 
TCAGCAGATG GCAGTGACCT GGATGCTGTT AGTCATGGCA GCATGGATAG TGGTCATGGG 
ACACACACTG TGGAGCAGGC ACCTTTTAAT ACGGGTTTAA TCAAAGTATA TGCTACTGAT 
GATAGATCTA GTACAGGTGG CCAGTCTGAT CTTGGATATA ATTCATTATC TAAGGATGAA 
GTTAGAAGAG GGGATATATC TACTGAGGAA ATTCAAGAAG AAAAAGATAA GAAAGGAAGT 
GATTCTAGTT CCCTGTCAGA GAGTGAGAGT ACAAAAGGAA GTGCTGACTG TCTTCCTAAA 
CTCAATTATC AAAGTTCTTC CAGTATAGTT CGCCTCAGTG GTACAAATAA CAATGGTGCT 
GATAAAATAT CAGGAGAAAG CACAGAAAGC ACATCTAAGC TGCTCTTACT ATCATCTTTT 
GAGGGTACAA ATGAAACAGG AAATAGTCAA AATAGATGCT TCAGGAAAAG ACATAAAAGT 
GACAACGAAC CTAATTTGCA ACAGCGACTC TCCTGGGGAA GCAGAAACCG CAATCTTAGT 
GGAGGAGTGC TGATGGGACT TATGCTTAAT AGAATCAACC AGGAAGCAAA CCCTGGAGAC 
ATGGTTGAAA AACTAGGAGC TGATGCAAAA ATTCTTTCAA ACGTTATATC ACAAAGCACA 
AGACCAACTA GTCTTGATAT TGGAAAACCC CCTTTAAGAT CAAAAAGAGA CAGTCTAGAA 
AAGGAATCTA GCGACGATGA TACACCTTTC GATGGTTCTA ATTGTTTGGC AGATAAAGTG 
GATTCTCCTG TTATTTTTGA TTTGGAAGAC TTAGACAGTG AGACAGATGG ATCAAAAGTG 
GGATACATTG CTTCACAAAA TCCCAGAAGG ATTCAGCGTA TGAGCAGCAG CTTCTCGGTA 
AAACCTTCTG AAAAAACGGA TGTTGCCACA GGATTTGATC CCCTCTCTCT TTTGGTTGCT 
GAGACTGAAC AGCAGCAAAA AGAGGAAGAG GAGGAGGATG ATGAAGATAG CAAAAGCGTT 
TCAACACCAT CCGCCAGGCG TGACTTAGCC GAAGAGATTG TGATGTATAT GAATAACATG 
AGCAGTCCTT TGACAAGCCG CACACCAAGC ATCGATTTAC AACGGGCATG TGATGACAAA 
TCAACCAGTA AGAAAAGTCC AACATTTGTC AAGGCATGCA GGAGATCTAG CCTGCCTCCT 
CATTCTCCGA GGCCAGTGAG ACTTACCAAA TCTAAAAGTT ATACAAAAAC TGAGGAGAGA 
CCTCGGGACA GACTCTGGTC TTCTCCAGCC TTCTCACCTA CTTGCCCATT TAGGGAAGAA 
TCTCAAGACG CATTAACCCA TTCATCACCT TCATTTAATT TAGATACACT GCTAGTACCT 
AAACTAGATG TTCTAAGGAA CAGTATGTTC ACTGCTGGGA AAGGAGTTGC CGAGAAAGCC 
AGCAAATGGT ATTCAAGATT CACCATGTAT ACCACATCAT CTAAGGATCA AAGTTCTGAT 
CGTACCAGTC TTTCTTCAGT GGGAGCCCAG GATTCTGAAT CTACCTCTTT AACAGATGAA 
GATGTTTGCC ATGAATTGGA AGGAGCCACT TCCTCCCAAG AGAGCTGTGC TGCTTCAGGG 
AATAAGGGAA TTGATTTCAG CAGAACAAGC CTGGAAAGTT CTGCCTCCTT AGAAGGATCC 
CTGTCAAAGT TTGCCTTACC TGGGAAATCA GAAGTGGCAT CTTCCTGCAA CACAAGTAAT 
ACAAATATCT TCCAGAACTA TGCAATGGAG GTTCTTATCT CAAGTTGCTC TCGGTGTAGA 
ACTTGTGATT GTCTTGTCCA TGATGAGGAA ATCATGGCTG GCTGGACAGC AGATGATTCA 
AATCTCAATA CTACATGCCC GTTTTGTGGC AATCTCTTCT TGCCCTTTCT GAATATTGAA 
ATAAGAGATT TAAGACGACC CGGAAGATAC TTTCTGAAGT CAAGTCCATC TACAGAAAAT 
ATGCATTTTC CATCCTCCCT TTCAAGTCAG ACAAGGCAGT CTTGTATCTC AACATCAGCC 
TCTGGTCTTG ACACGTCTGT TATCTCTGTT CAAGGGAATT TTGATCTCAA TAGCAAATCT 
AAACTGCTGG AAAATACATG TGCCCGAACT ATTCACATCC CTGCTGATAG ATCAAAAACA 
GGTGTGTCAA AATGTCCAAT ATTTCCAATG GCCAGGAGTG TTAGTACTTA TGGCCCCTTG 
GATAAGGAAG ACACTGGAGG CCAGAAGCTC ATTCCTACAG GCAGCCTGCC AACTACTTTA 
CAAGGAGCTA CAGATTCTTT AGGATTAGAG TGGCACCTTC CTAGTCCAGA TCCTATCACA 
GTTCCATATC TTAGTCCTTT AGTGGTATGG AAGGAGCTTG AAAGCTTACT GGAAAATGAA 
GGCGACCATG CAATAACAGT GGCAGACTTT GTGGATCATC ATCCAATCGT GTTTTGGAAC 
CTGGTGTGGT ATTTTAGACG TCTCGACTTA CCCAGTAACT TACCTGGATT GATTCTTTCT 
TCTGAGCACT GTAACAAGTA TTCAAAGATT CCTCGCCACT GTATGTCGGA AGATAGTAAA 
TACGTTTTAA TACAAATGTT ATGGGACAAT ATGAAATTAC ATCAGGATCC GGGACAGCCT 
TTGTACATCC TCTGGAATGC CCACAGTCAA AATCGTACTC TTTTGTTTGA GACCCAAAAG 
TATCCAATGG TCCATTTATT GCAAAAGGGT GATAATTCAT TTAACCAGGA ACTGTTGAAA 
AATATGGTAA AAAGCATTAA AATGAATGAT GTCTATGGAC CAATGAGTCA AATTTTAGAG 
ACACTGAATA AATGTCCTCA TTTTAAAAGA CAGAGGAGTT TATATAGAGA AATACTATTT 
CTCTCACTTG TGGCACTGGG AAGAGAAAAC ATTGATATAG ATGCATTTGA TAAAGAATAC 
AAGATGGCAT ACGATCGTCT CACACCTAGT CAAGTCAAGA GTACACACAA CTGTGATAGA 
CCACCAAGTA CAGGGGTGAT GGAATGTCGA AAAACCTTTG GAGAACCTTA TCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DENND4A ( XM_022412984.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022268692.1 Canis lupus familiaris DENN domain containing 4A (DENND4A), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OCe29018
Clone ID Related Accession (Same CDS sequence)	XM_005638527.3 , XM_022412983.1 , XM_005638527.2 , XM_022412984.1
Accession Version	XM_022412984.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5757bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	C-myc promoter-binding protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006612.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTGAAG ATAAGGGTCC TCGTGTTGCT GACTACTTTG TTGTAGCAGG ATTAACTGAT 
GTTTCAAAGC CTCTTGAAGA AGAAATTCAC TTCAATGATG CCTGTCATAA AGTAGCTAAA 
CCAAAAGAAC CTATCACAGA TGTTTCGGTG ATTATTAAAT CTCTTGGGGA AGAAGTGCCA 
CGGGATTATA TATGTATTGA TGTTACCCCA ACTGGATTGT CGGCTGATCT CAATAATGGA 
AGTCTTGTGG GGCCACAGAT TTACCTTTGC TACAGAAGAG GAAGAGATAA GCCTCCACTT 
ACAGATCTGG GGGTTTTGTA TGACTGGAAA GAAAGATTGA AACAGGGATG TGAAATTATT 
CAGAGTACTC CCTATGGACA CCCTGCAAAT ATTAGTGGCA GTACCTCATC ACAAAAAATT 
TATATAACTT ACCGAAGAGC CTCTGAAAAC ATGACGCAAA ATACGTTGGC TGTCACAGAC 
ATATGTATTA TTATACCCAG TAAAGGAGAG AGTCCACCAC ACACGTTTTG CAAAGTCGAT 
AAGAATCTCA ATAACAGTAT GTGGGGATCT GCAGTGTACT TATGTTATAA AAAATCAGTG 
GCAAAGACTA ACACTATATC CTATAAAGCT GGTCTAATTT GTAGATATCC TCAAGAAGAT 
TACGAATCTT TCTCACTCCC AGAATCTGTT CCCCTATTTT GTTTACCAAT GGGTGCAACT 
ATTGAATGTT GGCCACCAAA TAGCAAATAC CCTCTCCCTG TATTTTCTAC ATTTGTGTTA 
ACTGGAGCCT CAGCTGAAAA GGTCTATGGT GCTGCTATTC AGTTTTATGA ACCATACTCT 
GAGGAGAATC TCACAGAAAA ACAGAGACTT CTTTTGGGTT TAGCATCATC AGCAGATGGG 
AAATCTGATA GTTCTAAAAC AATTCACACT AGTAAATGCA TCTGTCTTCT TTCTCACTGG 
CCTTTTTTTG ATGCATTCAG GAAATTTTTG ACCTTTCTGT ATCGTTACTC TATCTCTGGA 
CCTCATGTCC TTCCAATTGA GAAGCATATT TCTCATTTTA TGCATAAAGT TCCTTTTCCA 
TCTCCTCAGA GGCCACGGAT TTTAGTTCAG TTATCGCCAC ATGATAATCT GATTCTCAGT 
CAACCTGTTT CTTCACCTCT TCCACTAAGT GGTGGCAAAT TTTCAACACT ACTTCAAAAT 
TTAGGCCCTG AAAATGCTGT AACACTACTG GTGTTTGCAG TAACAGAGCA CAAGATTCTC 
ATCCATTCCT TACGGCCTTC CGTGCTTACT AGCGTGACAG AAGCATTAGT GTCTATGATT 
TTCCCTTTCC ACTGGCCGTG CCCATATGTT CCTCTCTGCC CACTGGCTTT AGCAGATGTC 
TTGAGTGCAC CATGTCCATT CATTGTAGGA ATTGATTCAA GATATTTTGA TCTCTATGAC 
CCTCCACCAG ATGTTAGTTG TGTTGACCTA GATACCAACA CTATTTCTCA AACTGGCGAT 
AAGAAAAATG TAGCCTGGAA GATACTTCCA AAGAAACCAT GTAAAAACCT GATGAACACA 
CTGAATAATT TGCACCAGCA GTTGGCAAAA TTGCAGCAGA GACCAAGAGA TGATGGACTA 
ATGGACTTAG CAATGAATGA CTATGATTTT AATTCTGGAA AGAGGTTGCA TATGATAGAT 
TTGGAAATCC AAGAGGCATT TTTGTGTTTC ATGGCCTCTA TTTTAAAAGG TTATAGATCA 
TACTTAAGAC CAATAACACA AGCCCCTTCT GAGACAGCCA CCGATGCATC CTCTCTTTTT 
GCCCTACAAG CTTTCTTAAG AAGCCGAGAC CGGTCACATC AAAAATTCTA TAACATGATG 
ACCAAAACAC AAATGTTCAT TCGCTTCATT GAGGAATGTT CTTTTGTCAG TGATAAAGAT 
GCAAGCCTGG CATTTTTTGA TGATTGTGTA GATAAAGTGG ATATGGACAA GAGTGGTGAA 
ATGCGACTTA TAGAACTGGA TGAATCTTTC AAAAGTGAAC ATACTGTTTT TGTAACACCA 
CCTGAGATAC CCCATCTACC CAATGGTGAA GAACCCCCTT TACAGTACAG CTATAATGGA 
TTTCCAGTTC TTAGAAACAA TTTATTTGAG AGACCTGAAG GATTTCTACA AACACGAAAG 
AACAAATTGC CTTCAAAATC AAGTAGTCCT AGTAGCCCTT CTCCCATGTT CAGAAGAACA 
AAACAGGAAA TTAAATCAGC CCATAAAATT GCCAAGAGGT ACTCTTCCAT TCCTCAGATG 
TGGTCTAGGT GTCTGCTGCG CCACTGCTAT GGACTGTGGT TCATTTGTCT GCCAGCTTAT 
GTGAAAGTGT GTCATTCAAA AGTCAGGGCA CTGAAGACAG CATATGATGT GCTCAAAAAA 
ATGCAGTCGA AAAAGATGGA TCCACCTGAT GAAGTGTGCT ACCGCATTCT CATGCAGCTC 
TGTGGGCAGT ACGATCAGCC CGTGCTTGCA GTTCGAGTGC TTTTTGAAAT GCAGAAAGCT 
GGTATTGATC CCAATGCCAT TACTTATGGT TATTATAATA AGGCTGTTTT GGAAAGCACC 
TGGCCCTCAA GAAGTCGTAG TGGCTATTTT CTTTGGACAA AAGTAAGAAA CGTTGTTTTA 
GGAGTAGCAC AGTTCAAAAG AGCTTTAAAG AAACAGGCAC ACTTATCACA AACAACTCTC 
TCAGCAGATG GCAGTGACCT GGATGCTGTT AGTCATGGCA GCATGGATAG TGGTCATGGG 
ACACACACTG TGGAGCAGGC ACCTTTTAAT ACGGGTTTAA TCAAAGTATA TGCTACTGAT 
GATAGATCTA GTACAGGTGG CCAGTCTGAT CTTGGATATA ATTCATTATC TAAGGATGAA 
GTTAGAAGAG GGGATATATC TACTGAGGAA ATTCAAGAAG AAAAAGATAA GAAAGGAAGT 
GATTCTAGTT CCCTGTCAGA GAGTGAGAGT ACAAAAGGAA GTGCTGACTG TCTTCCTAAA 
CTCAATTATC AAAGTTCTTC CAGTATAGTT CGCCTCAGTG GTACAAATAA CAATGGTGCT 
GATAAAATAT CAGGAGAAAG CACAGAAAGC ACATCTAAGC TGCTCTTACT ATCATCTTTT 
GAGGGTACAA ATGAAACAGG AAATAGTCAA AATAGATGCT TCAGGAAAAG ACATAAAAGT 
GACAACGAAC CTAATTTGCA ACAGCGACTC TCCTGGGGAA GCAGAAACCG CAATCTTAGT 
GGAGGAGTGC TGATGGGACT TATGCTTAAT AGAATCAACC AGGAAGCAAA CCCTGGAGAC 
ATGGTTGAAA AACTAGGAGC TGATGCAAAA ATTCTTTCAA ACGTTATATC ACAAAGCACA 
AGACCAACTA GTCTTGATAT TGGAAAACCC CCTTTAAGAT CAAAAAGAGA CAGTCTAGAA 
AAGGAATCTA GCGACGATGA TACACCTTTC GATGGTTCTA ATTGTTTGGC AGATAAAGTG 
GATTCTCCTG TTATTTTTGA TTTGGAAGAC TTAGACAGTG AGACAGATGG ATCAAAAGTG 
GGATACATTG CTTCACAAAA TCCCAGAAGG ATTCAGCGTA TGAGCAGCAG CTTCTCGGTA 
AAACCTTCTG AAAAAACGGA TGTTGCCACA GGATTTGATC CCCTCTCTCT TTTGGTTGCT 
GAGACTGAAC AGCAGCAAAA AGAGGAAGAG GAGGAGGATG ATGAAGATAG CAAAAGCGTT 
TCAACACCAT CCGCCAGGCG TGACTTAGCC GAAGAGATTG TGATGTATAT GAATAACATG 
AGCAGTCCTT TGACAAGCCG CACACCAAGC ATCGATTTAC AACGGGCATG TGATGACAAA 
TCAACCAGTA AGAAAAGTCC AACATTTGTC AAGGCATGCA GGAGATCTAG CCTGCCTCCT 
CATTCTCCGA GGCCAGTGAG ACTTACCAAA TCTAAAAGTT ATACAAAAAC TGAGGAGAGA 
CCTCGGGACA GACTCTGGTC TTCTCCAGCC TTCTCACCTA CTTGCCCATT TAGGGAAGAA 
TCTCAAGACG CATTAACCCA TTCATCACCT TCATTTAATT TAGATACACT GCTAGTACCT 
AAACTAGATG TTCTAAGGAA CAGTATGTTC ACTGCTGGGA AAGGAGTTGC CGAGAAAGCC 
AGCAAATGGT ATTCAAGATT CACCATGTAT ACCACATCAT CTAAGGATCA AAGTTCTGAT 
CGTACCAGTC TTTCTTCAGT GGGAGCCCAG GATTCTGAAT CTACCTCTTT AACAGATGAA 
GATGTTTGCC ATGAATTGGA AGGAGCCACT TCCTCCCAAG AGAGCTGTGC TGCTTCAGGG 
AATAAGGGAA TTGATTTCAG CAGAACAAGC CTGGAAAGTT CTGCCTCCTT AGAAGGATCC 
CTGTCAAAGT TTGCCTTACC TGGGAAATCA GAAGTGGCAT CTTCCTGCAA CACAAGTAAT 
ACAAATATCT TCCAGAACTA TGCAATGGAG GTTCTTATCT CAAGTTGCTC TCGGTGTAGA 
ACTTGTGATT GTCTTGTCCA TGATGAGGAA ATCATGGCTG GCTGGACAGC AGATGATTCA 
AATCTCAATA CTACATGCCC GTTTTGTGGC AATCTCTTCT TGCCCTTTCT GAATATTGAA 
ATAAGAGATT TAAGACGACC CGGAAGATAC TTTCTGAAGT CAAGTCCATC TACAGAAAAT 
ATGCATTTTC CATCCTCCCT TTCAAGTCAG ACAAGGCAGT CTTGTATCTC AACATCAGCC 
TCTGGTCTTG ACACGTCTGT TATCTCTGTT CAAGGGAATT TTGATCTCAA TAGCAAATCT 
AAACTGCTGG AAAATACATG TGCCCGAACT ATTCACATCC CTGCTGATAG ATCAAAAACA 
GGTGTGTCAA AATGTCCAAT ATTTCCAATG GCCAGGAGTG TTAGTACTTA TGGCCCCTTG 
GATAAGGAAG ACACTGGAGG CCAGAAGCTC ATTCCTACAG GCAGCCTGCC AACTACTTTA 
CAAGGAGCTA CAGATTCTTT AGGATTAGAG TGGCACCTTC CTAGTCCAGA TCCTATCACA 
GTTCCATATC TTAGTCCTTT AGTGGTATGG AAGGAGCTTG AAAGCTTACT GGAAAATGAA 
GGCGACCATG CAATAACAGT GGCAGACTTT GTGGATCATC ATCCAATCGT GTTTTGGAAC 
CTGGTGTGGT ATTTTAGACG TCTCGACTTA CCCAGTAACT TACCTGGATT GATTCTTTCT 
TCTGAGCACT GTAACAAGTA TTCAAAGATT CCTCGCCACT GTATGTCGGA AGATAGTAAA 
TACGTTTTAA TACAAATGTT ATGGGACAAT ATGAAATTAC ATCAGGATCC GGGACAGCCT 
TTGTACATCC TCTGGAATGC CCACAGTCAA AATCGTACTC TTTTGTTTGA GACCCAAAAG 
TATCCAATGG TCCATTTATT GCAAAAGGGT GATAATTCAT TTAACCAGGA ACTGTTGAAA 
AATATGGTAA AAAGCATTAA AATGAATGAT GTCTATGGAC CAATGAGTCA AATTTTAGAG 
ACACTGAATA AATGTCCTCA TTTTAAAAGA CAGAGGAGTT TATATAGAGA AATACTATTT 
CTCTCACTTG TGGCACTGGG AAGAGAAAAC ATTGATATAG ATGCATTTGA TAAAGAATAC 
AAGATGGCAT ACGATCGTCT CACACCTAGT CAAGTCAAGA GTACACACAA CTGTGATAGA 
CCACCAAGTA CAGGGGTGAT GGAATGTCGA AAAACCTTTG GAGAACCTTA TCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022268692.1
CDS	394..6150
Translation	MIEDKGPRVADYFVVAGLTDVSKPLEEEIHFNDACHKVAKPKEPITDVSVIIKSLGEEVPRDYICIDVTPTGLSADLNNGSLVGPQIYLCYRRGRDKPPLTDLGVLYDWKERLKQGCEIIQSTPYGHPANISGSTSSQKIYITYRRASENMTQNTLAVTDICIIIPSKGESPPHTFCKVDKNLNNSMWGSAVYLCYKKSVAKTNTISYKAGLICRYPQEDYESFSLPESVPLFCLPMGATIECWPPNSKYPLPVFSTFVLTGASAEKVYGAAIQFYEPYSEENLTEKQRLLLGLASSADGKSDSSKTIHTSKCICLLSHWPFFDAFRKFLTFLYRYSISGPHVLPIEKHISHFMHKVPFPSPQRPRILVQLSPHDNLILSQPVSSPLPLSGGKFSTLLQNLGPENAVTLLVFAVTEHKILIHSLRPSVLTSVTEALVSMIFPFHWPCPYVPLCPLALADVLSAPCPFIVGIDSRYFDLYDPPPDVSCVDLDTNTISQTGDKKNVAWKILPKKPCKNLMNTLNNLHQQLAKLQQRPRDDGLMDLAMNDYDFNSGKRLHMIDLEIQEAFLCFMASILKGYRSYLRPITQAPSETATDASSLFALQAFLRSRDRSHQKFYNMMTKTQMFIRFIEECSFVSDKDASLAFFDDCVDKVDMDKSGEMRLIELDESFKSEHTVFVTPPEIPHLPNGEEPPLQYSYNGFPVLRNNLFERPEGFLQTRKNKLPSKSSSPSSPSPMFRRTKQEIKSAHKIAKRYSSIPQMWSRCLLRHCYGLWFICLPAYVKVCHSKVRALKTAYDVLKKMQSKKMDPPDEVCYRILMQLCGQYDQPVLAVRVLFEMQKAGIDPNAITYGYYNKAVLESTWPSRSRSGYFLWTKVRNVVLGVAQFKRALKKQAHLSQTTLSADGSDLDAVSHGSMDSGHGTHTVEQAPFNTGLIKVYATDDRSSTGGQSDLGYNSLSKDEVRRGDISTEEIQEEKDKKGSDSSSLSESESTKGSADCLPKLNYQSSSSIVRLSGTNNNGADKISGESTESTSKLLLLSSFEGTNETGNSQNRCFRKRHKSDNEPNLQQRLSWGSRNRNLSGGVLMGLMLNRINQEANPGDMVEKLGADAKILSNVISQSTRPTSLDIGKPPLRSKRDSLEKESSDDDTPFDGSNCLADKVDSPVIFDLEDLDSETDGSKVGYIASQNPRRIQRMSSSFSVKPSEKTDVATGFDPLSLLVAETEQQQKEEEEEDDEDSKSVSTPSARRDLAEEIVMYMNNMSSPLTSRTPSIDLQRACDDKSTSKKSPTFVKACRRSSLPPHSPRPVRLTKSKSYTKTEERPRDRLWSSPAFSPTCPFREESQDALTHSSPSFNLDTLLVPKLDVLRNSMFTAGKGVAEKASKWYSRFTMYTTSSKDQSSDRTSLSSVGAQDSESTSLTDEDVCHELEGATSSQESCAASGNKGIDFSRTSLESSASLEGSLSKFALPGKSEVASSCNTSNTNIFQNYAMEVLISSCSRCRTCDCLVHDEEIMAGWTADDSNLNTTCPFCGNLFLPFLNIEIRDLRRPGRYFLKSSPSTENMHFPSSLSSQTRQSCISTSASGLDTSVISVQGNFDLNSKSKLLENTCARTIHIPADRSKTGVSKCPIFPMARSVSTYGPLDKEDTGGQKLIPTGSLPTTLQGATDSLGLEWHLPSPDPITVPYLSPLVVWKELESLLENEGDHAITVADFVDHHPIVFWNLVWYFRRLDLPSNLPGLILSSEHCNKYSKIPRHCMSEDSKYVLIQMLWDNMKLHQDPGQPLYILWNAHSQNRTLLFETQKYPMVHLLQKGDNSFNQELLKNMVKSIKMNDVYGPMSQILETLNKCPHFKRQRSLYREILFLSLVALGRENIDIDAFDKEYKMAYDRLTPSQVKSTHNCDRPPSTGVMECRKTFGEPYL

Target ORF information:

RefSeq Version	XM_022412984.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris DENN domain containing 4A (DENND4A), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022412984.1

ORF Insert Sequence:

ATGATTGAAG ATAAGGGTCC TCGTGTTGCT GACTACTTTG TTGTAGCAGG ATTAACTGAT 
GTTTCAAAGC CTCTTGAAGA AGAAATTCAC TTCAATGATG CCTGTCATAA AGTAGCTAAA 
CCAAAAGAAC CTATCACAGA TGTTTCGGTG ATTATTAAAT CTCTTGGGGA AGAAGTGCCA 
CGGGATTATA TATGTATTGA TGTTACCCCA ACTGGATTGT CGGCTGATCT CAATAATGGA 
AGTCTTGTGG GGCCACAGAT TTACCTTTGC TACAGAAGAG GAAGAGATAA GCCTCCACTT 
ACAGATCTGG GGGTTTTGTA TGACTGGAAA GAAAGATTGA AACAGGGATG TGAAATTATT 
CAGAGTACTC CCTATGGACA CCCTGCAAAT ATTAGTGGCA GTACCTCATC ACAAAAAATT 
TATATAACTT ACCGAAGAGC CTCTGAAAAC ATGACGCAAA ATACGTTGGC TGTCACAGAC 
ATATGTATTA TTATACCCAG TAAAGGAGAG AGTCCACCAC ACACGTTTTG CAAAGTCGAT 
AAGAATCTCA ATAACAGTAT GTGGGGATCT GCAGTGTACT TATGTTATAA AAAATCAGTG 
GCAAAGACTA ACACTATATC CTATAAAGCT GGTCTAATTT GTAGATATCC TCAAGAAGAT 
TACGAATCTT TCTCACTCCC AGAATCTGTT CCCCTATTTT GTTTACCAAT GGGTGCAACT 
ATTGAATGTT GGCCACCAAA TAGCAAATAC CCTCTCCCTG TATTTTCTAC ATTTGTGTTA 
ACTGGAGCCT CAGCTGAAAA GGTCTATGGT GCTGCTATTC AGTTTTATGA ACCATACTCT 
GAGGAGAATC TCACAGAAAA ACAGAGACTT CTTTTGGGTT TAGCATCATC AGCAGATGGG 
AAATCTGATA GTTCTAAAAC AATTCACACT AGTAAATGCA TCTGTCTTCT TTCTCACTGG 
CCTTTTTTTG ATGCATTCAG GAAATTTTTG ACCTTTCTGT ATCGTTACTC TATCTCTGGA 
CCTCATGTCC TTCCAATTGA GAAGCATATT TCTCATTTTA TGCATAAAGT TCCTTTTCCA 
TCTCCTCAGA GGCCACGGAT TTTAGTTCAG TTATCGCCAC ATGATAATCT GATTCTCAGT 
CAACCTGTTT CTTCACCTCT TCCACTAAGT GGTGGCAAAT TTTCAACACT ACTTCAAAAT 
TTAGGCCCTG AAAATGCTGT AACACTACTG GTGTTTGCAG TAACAGAGCA CAAGATTCTC 
ATCCATTCCT TACGGCCTTC CGTGCTTACT AGCGTGACAG AAGCATTAGT GTCTATGATT 
TTCCCTTTCC ACTGGCCGTG CCCATATGTT CCTCTCTGCC CACTGGCTTT AGCAGATGTC 
TTGAGTGCAC CATGTCCATT CATTGTAGGA ATTGATTCAA GATATTTTGA TCTCTATGAC 
CCTCCACCAG ATGTTAGTTG TGTTGACCTA GATACCAACA CTATTTCTCA AACTGGCGAT 
AAGAAAAATG TAGCCTGGAA GATACTTCCA AAGAAACCAT GTAAAAACCT GATGAACACA 
CTGAATAATT TGCACCAGCA GTTGGCAAAA TTGCAGCAGA GACCAAGAGA TGATGGACTA 
ATGGACTTAG CAATGAATGA CTATGATTTT AATTCTGGAA AGAGGTTGCA TATGATAGAT 
TTGGAAATCC AAGAGGCATT TTTGTGTTTC ATGGCCTCTA TTTTAAAAGG TTATAGATCA 
TACTTAAGAC CAATAACACA AGCCCCTTCT GAGACAGCCA CCGATGCATC CTCTCTTTTT 
GCCCTACAAG CTTTCTTAAG AAGCCGAGAC CGGTCACATC AAAAATTCTA TAACATGATG 
ACCAAAACAC AAATGTTCAT TCGCTTCATT GAGGAATGTT CTTTTGTCAG TGATAAAGAT 
GCAAGCCTGG CATTTTTTGA TGATTGTGTA GATAAAGTGG ATATGGACAA GAGTGGTGAA 
ATGCGACTTA TAGAACTGGA TGAATCTTTC AAAAGTGAAC ATACTGTTTT TGTAACACCA 
CCTGAGATAC CCCATCTACC CAATGGTGAA GAACCCCCTT TACAGTACAG CTATAATGGA 
TTTCCAGTTC TTAGAAACAA TTTATTTGAG AGACCTGAAG GATTTCTACA AACACGAAAG 
AACAAATTGC CTTCAAAATC AAGTAGTCCT AGTAGCCCTT CTCCCATGTT CAGAAGAACA 
AAACAGGAAA TTAAATCAGC CCATAAAATT GCCAAGAGGT ACTCTTCCAT TCCTCAGATG 
TGGTCTAGGT GTCTGCTGCG CCACTGCTAT GGACTGTGGT TCATTTGTCT GCCAGCTTAT 
GTGAAAGTGT GTCATTCAAA AGTCAGGGCA CTGAAGACAG CATATGATGT GCTCAAAAAA 
ATGCAGTCGA AAAAGATGGA TCCACCTGAT GAAGTGTGCT ACCGCATTCT CATGCAGCTC 
TGTGGGCAGT ACGATCAGCC CGTGCTTGCA GTTCGAGTGC TTTTTGAAAT GCAGAAAGCT 
GGTATTGATC CCAATGCCAT TACTTATGGT TATTATAATA AGGCTGTTTT GGAAAGCACC 
TGGCCCTCAA GAAGTCGTAG TGGCTATTTT CTTTGGACAA AAGTAAGAAA CGTTGTTTTA 
GGAGTAGCAC AGTTCAAAAG AGCTTTAAAG AAACAGGCAC ACTTATCACA AACAACTCTC 
TCAGCAGATG GCAGTGACCT GGATGCTGTT AGTCATGGCA GCATGGATAG TGGTCATGGG 
ACACACACTG TGGAGCAGGC ACCTTTTAAT ACGGGTTTAA TCAAAGTATA TGCTACTGAT 
GATAGATCTA GTACAGGTGG CCAGTCTGAT CTTGGATATA ATTCATTATC TAAGGATGAA 
GTTAGAAGAG GGGATATATC TACTGAGGAA ATTCAAGAAG AAAAAGATAA GAAAGGAAGT 
GATTCTAGTT CCCTGTCAGA GAGTGAGAGT ACAAAAGGAA GTGCTGACTG TCTTCCTAAA 
CTCAATTATC AAAGTTCTTC CAGTATAGTT CGCCTCAGTG GTACAAATAA CAATGGTGCT 
GATAAAATAT CAGGAGAAAG CACAGAAAGC ACATCTAAGC TGCTCTTACT ATCATCTTTT 
GAGGGTACAA ATGAAACAGG AAATAGTCAA AATAGATGCT TCAGGAAAAG ACATAAAAGT 
GACAACGAAC CTAATTTGCA ACAGCGACTC TCCTGGGGAA GCAGAAACCG CAATCTTAGT 
GGAGGAGTGC TGATGGGACT TATGCTTAAT AGAATCAACC AGGAAGCAAA CCCTGGAGAC 
ATGGTTGAAA AACTAGGAGC TGATGCAAAA ATTCTTTCAA ACGTTATATC ACAAAGCACA 
AGACCAACTA GTCTTGATAT TGGAAAACCC CCTTTAAGAT CAAAAAGAGA CAGTCTAGAA 
AAGGAATCTA GCGACGATGA TACACCTTTC GATGGTTCTA ATTGTTTGGC AGATAAAGTG 
GATTCTCCTG TTATTTTTGA TTTGGAAGAC TTAGACAGTG AGACAGATGG ATCAAAAGTG 
GGATACATTG CTTCACAAAA TCCCAGAAGG ATTCAGCGTA TGAGCAGCAG CTTCTCGGTA 
AAACCTTCTG AAAAAACGGA TGTTGCCACA GGATTTGATC CCCTCTCTCT TTTGGTTGCT 
GAGACTGAAC AGCAGCAAAA AGAGGAAGAG GAGGAGGATG ATGAAGATAG CAAAAGCGTT 
TCAACACCAT CCGCCAGGCG TGACTTAGCC GAAGAGATTG TGATGTATAT GAATAACATG 
AGCAGTCCTT TGACAAGCCG CACACCAAGC ATCGATTTAC AACGGGCATG TGATGACAAA 
TCAACCAGTA AGAAAAGTCC AACATTTGTC AAGGCATGCA GGAGATCTAG CCTGCCTCCT 
CATTCTCCGA GGCCAGTGAG ACTTACCAAA TCTAAAAGTT ATACAAAAAC TGAGGAGAGA 
CCTCGGGACA GACTCTGGTC TTCTCCAGCC TTCTCACCTA CTTGCCCATT TAGGGAAGAA 
TCTCAAGACG CATTAACCCA TTCATCACCT TCATTTAATT TAGATACACT GCTAGTACCT 
AAACTAGATG TTCTAAGGAA CAGTATGTTC ACTGCTGGGA AAGGAGTTGC CGAGAAAGCC 
AGCAAATGGT ATTCAAGATT CACCATGTAT ACCACATCAT CTAAGGATCA AAGTTCTGAT 
CGTACCAGTC TTTCTTCAGT GGGAGCCCAG GATTCTGAAT CTACCTCTTT AACAGATGAA 
GATGTTTGCC ATGAATTGGA AGGAGCCACT TCCTCCCAAG AGAGCTGTGC TGCTTCAGGG 
AATAAGGGAA TTGATTTCAG CAGAACAAGC CTGGAAAGTT CTGCCTCCTT AGAAGGATCC 
CTGTCAAAGT TTGCCTTACC TGGGAAATCA GAAGTGGCAT CTTCCTGCAA CACAAGTAAT 
ACAAATATCT TCCAGAACTA TGCAATGGAG GTTCTTATCT CAAGTTGCTC TCGGTGTAGA 
ACTTGTGATT GTCTTGTCCA TGATGAGGAA ATCATGGCTG GCTGGACAGC AGATGATTCA 
AATCTCAATA CTACATGCCC GTTTTGTGGC AATCTCTTCT TGCCCTTTCT GAATATTGAA 
ATAAGAGATT TAAGACGACC CGGAAGATAC TTTCTGAAGT CAAGTCCATC TACAGAAAAT 
ATGCATTTTC CATCCTCCCT TTCAAGTCAG ACAAGGCAGT CTTGTATCTC AACATCAGCC 
TCTGGTCTTG ACACGTCTGT TATCTCTGTT CAAGGGAATT TTGATCTCAA TAGCAAATCT 
AAACTGCTGG AAAATACATG TGCCCGAACT ATTCACATCC CTGCTGATAG ATCAAAAACA 
GGTGTGTCAA AATGTCCAAT ATTTCCAATG GCCAGGAGTG TTAGTACTTA TGGCCCCTTG 
GATAAGGAAG ACACTGGAGG CCAGAAGCTC ATTCCTACAG GCAGCCTGCC AACTACTTTA 
CAAGGAGCTA CAGATTCTTT AGGATTAGAG TGGCACCTTC CTAGTCCAGA TCCTATCACA 
GTTCCATATC TTAGTCCTTT AGTGGTATGG AAGGAGCTTG AAAGCTTACT GGAAAATGAA 
GGCGACCATG CAATAACAGT GGCAGACTTT GTGGATCATC ATCCAATCGT GTTTTGGAAC 
CTGGTGTGGT ATTTTAGACG TCTCGACTTA CCCAGTAACT TACCTGGATT GATTCTTTCT 
TCTGAGCACT GTAACAAGTA TTCAAAGATT CCTCGCCACT GTATGTCGGA AGATAGTAAA 
TACGTTTTAA TACAAATGTT ATGGGACAAT ATGAAATTAC ATCAGGATCC GGGACAGCCT 
TTGTACATCC TCTGGAATGC CCACAGTCAA AATCGTACTC TTTTGTTTGA GACCCAAAAG 
TATCCAATGG TCCATTTATT GCAAAAGGGT GATAATTCAT TTAACCAGGA ACTGTTGAAA 
AATATGGTAA AAAGCATTAA AATGAATGAT GTCTATGGAC CAATGAGTCA AATTTTAGAG 
ACACTGAATA AATGTCCTCA TTTTAAAAGA CAGAGGAGTT TATATAGAGA AATACTATTT 
CTCTCACTTG TGGCACTGGG AAGAGAAAAC ATTGATATAG ATGCATTTGA TAAAGAATAC 
AAGATGGCAT ACGATCGTCT CACACCTAGT CAAGTCAAGA GTACACACAA CTGTGATAGA 
CCACCAAGTA CAGGGGTGAT GGAATGTCGA AAAACCTTTG GAGAACCTTA TCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DENND4A ( XM_005638528.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005638585.1 Canis lupus familiaris DENN/MADD domain containing 4A (DENND4A), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCe29019
Clone ID Related Accession (Same CDS sequence)	XM_005638528.2 , XM_005638528.3
Accession Version	XM_005638528.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5754bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	C-myc promoter-binding protein isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726113.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005638528.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTGAAG ATAAGGGTCC TCGTGTTGCT GACTACTTTG TTGTAGCAGG ATTAACTGAT 
GTTTCAAAGC CTCTTGAAGA AGAAATTCAC TTCAATGATG CCTGTCATAA AGTAGCTAAA 
CCAAAAGAAC CTATCACAGA TGTTTCGGTG ATTATTAAAT CTCTTGGGGA AGAAGTGCCA 
CGGGATTATA TATGTATTGA TGTTACCCCA ACTGGATTGT CGGCTGATCT CAATAATGGA 
AGTCTTGTGG GGCCACAGAT TTACCTTTGC TACAGAAGAG GAAGAGATAA GCCTCCACTT 
ACAGATCTGG GGGTTTTGTA TGACTGGAAA GAAAGATTGA AACAGGGATG TGAAATTATT 
CAGAGTACTC CCTATGGACA CCCTGCAAAT ATTAGTGGCA GTACCTCATC ACAAAAAATT 
TATATAACTT ACCGAAGAGC CTCTGAAAAC ATGACGCAAA ATACGTTGGC TGTCACAGAC 
ATATGTATTA TTATACCCAG TAAAGGAGAG AGTCCACCAC ACACGTTTTG CAAAGTCGAT 
AAGAATCTCA ATAACAGTAT GTGGGGATCT GCAGTGTACT TATGTTATAA AAAATCAGTG 
GCAAAGACTA ACACTATATC CTATAAAGCT GGTCTAATTT GTAGATATCC TCAAGAAGAT 
TACGAATCTT TCTCACTCCC AGAATCTGTT CCCCTATTTT GTTTACCAAT GGGTGCAACT 
ATTGAATGTT GGCCACCAAA TAGCAAATAC CCTCTCCCTG TATTTTCTAC ATTTGTGTTA 
ACTGGAGCCT CAGCTGAAAA GGTCTATGGT GCTGCTATTC AGTTTTATGA ACCATACTCT 
GAGGAGAATC TCACAGAAAA ACAGAGACTT CTTTTGGGTT TAGCATCATC AGCAGATGGG 
AAATCTGATA GTTCTAAAAC AATTCACACT AGTAAATGCA TCTGTCTTCT TTCTCACTGG 
CCTTTTTTTG ATGCATTCAG GAAATTTTTG ACCTTTCTGT ATCGTTACTC TATCTCTGGA 
CCTCATGTCC TTCCAATTGA GAAGCATATT TCTCATTTTA TGCATAAAGT TCCTTTTCCA 
TCTCCTCAGA GGCCACGGAT TTTAGTTCAG TTATCGCCAC ATGATAATCT GATTCTCAGT 
CAACCTGTTT CTTCACCTCT TCCACTAAGT GGTGGCAAAT TTTCAACACT ACTTCAAAAT 
TTAGGCCCTG AAAATGCTGT AACACTACTG GTGTTTGCAG TAACAGAGCA CAAGATTCTC 
ATCCATTCCT TACGGCCTTC CGTGCTTACT AGCGTGACAG AAGCATTAGT GTCTATGATT 
TTCCCTTTCC ACTGGCCGTG CCCATATGTT CCTCTCTGCC CACTGGCTTT AGCAGATGTC 
TTGAGTGCAC CATGTCCATT CATTGTAGGA ATTGATTCAA GATATTTTGA TCTCTATGAC 
CCTCCACCAG ATGTTAGTTG TGTTGACCTA GATACCAACA CTATTTCTCA AACTGGCGAT 
AAGAAAAATG TAGCCTGGAA GATACTTCCA AAGAAACCAT GTAAAAACCT GATGAACACA 
CTGAATAATT TGCACCAGCA GTTGGCAAAA TTGCAGCAGA GACCAAGAGA TGATGGACTA 
ATGGACTTAG CAATGAATGA CTATGATTTT AATTCTGGAA AGAGGTTGCA TATGATAGAT 
TTGGAAATCC AAGAGGCATT TTTGTGTTTC ATGGCCTCTA TTTTAAAAGG TTATAGATCA 
TACTTAAGAC CAATAACACA AGCCCCTTCT GAGACAGCCA CCGATGCATC CTCTCTTTTT 
GCCCTACAAG CTTTCTTAAG AAGCCGAGAC CGGTCACATC AAAAATTCTA TAACATGATG 
ACCAAAACAC AAATGTTCAT TCGCTTCATT GAGGAATGTT CTTTTGTCAG TGATAAAGAT 
GCAAGCCTGG CATTTTTTGA TGATTGTGTA GATAAAGTGG ATATGGACAA GAGTGGTGAA 
ATGCGACTTA TAGAACTGGA TGAATCTTTC AAAAGTGAAC ATACTGTTTT TGTAACACCA 
CCTGAGATAC CCCATCTACC CAATGGTGAA GAACCCCCTT TACAGTACAG CTATAATGGA 
TTTCCAGTTC TTAGAAACAA TTTATTTGAG AGACCTGAAG GATTTCTACA AACACGAAAG 
AACAAATTGC CTTCAAAATC AAGTAGTCCT AGTAGCCCTT CTCCCATGTT CAGAAGAACA 
AAACAGGAAA TTAAATCAGC CCATAAAATT GCCAAGAGGT ACTCTTCCAT TCCTCAGATG 
TGGTCTAGGT GTCTGCTGCG CCACTGCTAT GGACTGTGGT TCATTTGTCT GCCAGCTTAT 
GTGAAAGTGT GTCATTCAAA AGTCAGGGCA CTGAAGACAG CATATGATGT GCTCAAAAAA 
ATGCAGTCGA AAAAGATGGA TCCACCTGAT GAAGTGTGCT ACCGCATTCT CATGCAGCTC 
TGTGGGCAGT ACGATCAGCC CGTGCTTGCA GTTCGAGTGC TTTTTGAAAT GCAGAAAGCT 
GGTATTGATC CCAATGCCAT TACTTATGGT TATTATAATA AGGCTGTTTT GGAAAGCACC 
TGGCCCTCAA GAAGTCGTAG TGGCTATTTT CTTTGGACAA AAGTAAGAAA CGTTGTTTTA 
GGAGTAGCAC AGTTCAAAAG AGCTTTAAAG AAACAGGCAC ACTTATCACA AACAACTCTC 
TCAGATGGCA GTGACCTGGA TGCTGTTAGT CATGGCAGCA TGGATAGTGG TCATGGGACA 
CACACTGTGG AGCAGGCACC TTTTAATACG GGTTTAATCA AAGTATATGC TACTGATGAT 
AGATCTAGTA CAGGTGGCCA GTCTGATCTT GGATATAATT CATTATCTAA GGATGAAGTT 
AGAAGAGGGG ATATATCTAC TGAGGAAATT CAAGAAGAAA AAGATAAGAA AGGAAGTGAT 
TCTAGTTCCC TGTCAGAGAG TGAGAGTACA AAAGGAAGTG CTGACTGTCT TCCTAAACTC 
AATTATCAAA GTTCTTCCAG TATAGTTCGC CTCAGTGGTA CAAATAACAA TGGTGCTGAT 
AAAATATCAG GAGAAAGCAC AGAAAGCACA TCTAAGCTGC TCTTACTATC ATCTTTTGAG 
GGTACAAATG AAACAGGAAA TAGTCAAAAT AGATGCTTCA GGAAAAGACA TAAAAGTGAC 
AACGAACCTA ATTTGCAACA GCGACTCTCC TGGGGAAGCA GAAACCGCAA TCTTAGTGGA 
GGAGTGCTGA TGGGACTTAT GCTTAATAGA ATCAACCAGG AAGCAAACCC TGGAGACATG 
GTTGAAAAAC TAGGAGCTGA TGCAAAAATT CTTTCAAACG TTATATCACA AAGCACAAGA 
CCAACTAGTC TTGATATTGG AAAACCCCCT TTAAGATCAA AAAGAGACAG TCTAGAAAAG 
GAATCTAGCG ACGATGATAC ACCTTTCGAT GGTTCTAATT GTTTGGCAGA TAAAGTGGAT 
TCTCCTGTTA TTTTTGATTT GGAAGACTTA GACAGTGAGA CAGATGGATC AAAAGTGGGA 
TACATTGCTT CACAAAATCC CAGAAGGATT CAGCGTATGA GCAGCAGCTT CTCGGTAAAA 
CCTTCTGAAA AAACGGATGT TGCCACAGGA TTTGATCCCC TCTCTCTTTT GGTTGCTGAG 
ACTGAACAGC AGCAAAAAGA GGAAGAGGAG GAGGATGATG AAGATAGCAA AAGCGTTTCA 
ACACCATCCG CCAGGCGTGA CTTAGCCGAA GAGATTGTGA TGTATATGAA TAACATGAGC 
AGTCCTTTGA CAAGCCGCAC ACCAAGCATC GATTTACAAC GGGCATGTGA TGACAAATCA 
ACCAGTAAGA AAAGTCCAAC ATTTGTCAAG GCATGCAGGA GATCTAGCCT GCCTCCTCAT 
TCTCCGAGGC CAGTGAGACT TACCAAATCT AAAAGTTATA CAAAAACTGA GGAGAGACCT 
CGGGACAGAC TCTGGTCTTC TCCAGCCTTC TCACCTACTT GCCCATTTAG GGAAGAATCT 
CAAGACGCAT TAACCCATTC ATCACCTTCA TTTAATTTAG ATACACTGCT AGTACCTAAA 
CTAGATGTTC TAAGGAACAG TATGTTCACT GCTGGGAAAG GAGTTGCCGA GAAAGCCAGC 
AAATGGTATT CAAGATTCAC CATGTATACC ACATCATCTA AGGATCAAAG TTCTGATCGT 
ACCAGTCTTT CTTCAGTGGG AGCCCAGGAT TCTGAATCTA CCTCTTTAAC AGATGAAGAT 
GTTTGCCATG AATTGGAAGG AGCCACTTCC TCCCAAGAGA GCTGTGCTGC TTCAGGGAAT 
AAGGGAATTG ATTTCAGCAG AACAAGCCTG GAAAGTTCTG CCTCCTTAGA AGGATCCCTG 
TCAAAGTTTG CCTTACCTGG GAAATCAGAA GTGGCATCTT CCTGCAACAC AAGTAATACA 
AATATCTTCC AGAACTATGC AATGGAGGTT CTTATCTCAA GTTGCTCTCG GTGTAGAACT 
TGTGATTGTC TTGTCCATGA TGAGGAAATC ATGGCTGGCT GGACAGCAGA TGATTCAAAT 
CTCAATACTA CATGCCCGTT TTGTGGCAAT CTCTTCTTGC CCTTTCTGAA TATTGAAATA 
AGAGATTTAA GACGACCCGG AAGATACTTT CTGAAGTCAA GTCCATCTAC AGAAAATATG 
CATTTTCCAT CCTCCCTTTC AAGTCAGACA AGGCAGTCTT GTATCTCAAC ATCAGCCTCT 
GGTCTTGACA CGTCTGTTAT CTCTGTTCAA GGGAATTTTG ATCTCAATAG CAAATCTAAA 
CTGCTGGAAA ATACATGTGC CCGAACTATT CACATCCCTG CTGATAGATC AAAAACAGGT 
GTGTCAAAAT GTCCAATATT TCCAATGGCC AGGAGTGTTA GTACTTATGG CCCCTTGGAT 
AAGGAAGACA CTGGAGGCCA GAAGCTCATT CCTACAGGCA GCCTGCCAAC TACTTTACAA 
GGAGCTACAG ATTCTTTAGG ATTAGAGTGG CACCTTCCTA GTCCAGATCC TATCACAGTT 
CCATATCTTA GTCCTTTAGT GGTATGGAAG GAGCTTGAAA GCTTACTGGA AAATGAAGGC 
GACCATGCAA TAACAGTGGC AGACTTTGTG GATCATCATC CAATCGTGTT TTGGAACCTG 
GTGTGGTATT TTAGACGTCT CGACTTACCC AGTAACTTAC CTGGATTGAT TCTTTCTTCT 
GAGCACTGTA ACAAGTATTC AAAGATTCCT CGCCACTGTA TGTCGGAAGA TAGTAAATAC 
GTTTTAATAC AAATGTTATG GGACAATATG AAATTACATC AGGATCCGGG ACAGCCTTTG 
TACATCCTCT GGAATGCCCA CAGTCAAAAT CGTACTCTTT TGTTTGAGAC CCAAAAGTAT 
CCAATGGTCC ATTTATTGCA AAAGGGTGAT AATTCATTTA ACCAGGAACT GTTGAAAAAT 
ATGGTAAAAA GCATTAAAAT GAATGATGTC TATGGACCAA TGAGTCAAAT TTTAGAGACA 
CTGAATAAAT GTCCTCATTT TAAAAGACAG AGGAGTTTAT ATAGAGAAAT ACTATTTCTC 
TCACTTGTGG CACTGGGAAG AGAAAACATT GATATAGATG CATTTGATAA AGAATACAAG 
ATGGCATACG ATCGTCTCAC ACCTAGTCAA GTCAAGAGTA CACACAACTG TGATAGACCA 
CCAAGTACAG GGGTGATGGA ATGTCGAAAA ACCTTTGGAG AACCTTATCT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005638585.1
CDS	378..6131
Translation	MIEDKGPRVADYFVVAGLTDVSKPLEEEIHFNDACHKVAKPKEPITDVSVIIKSLGEEVPRDYICIDVTPTGLSADLNNGSLVGPQIYLCYRRGRDKPPLTDLGVLYDWKERLKQGCEIIQSTPYGHPANISGSTSSQKIYITYRRASENMTQNTLAVTDICIIIPSKGESPPHTFCKVDKNLNNSMWGSAVYLCYKKSVAKTNTISYKAGLICRYPQEDYESFSLPESVPLFCLPMGATIECWPPNSKYPLPVFSTFVLTGASAEKVYGAAIQFYEPYSEENLTEKQRLLLGLASSADGKSDSSKTIHTSKCICLLSHWPFFDAFRKFLTFLYRYSISGPHVLPIEKHISHFMHKVPFPSPQRPRILVQLSPHDNLILSQPVSSPLPLSGGKFSTLLQNLGPENAVTLLVFAVTEHKILIHSLRPSVLTSVTEALVSMIFPFHWPCPYVPLCPLALADVLSAPCPFIVGIDSRYFDLYDPPPDVSCVDLDTNTISQTGDKKNVAWKILPKKPCKNLMNTLNNLHQQLAKLQQRPRDDGLMDLAMNDYDFNSGKRLHMIDLEIQEAFLCFMASILKGYRSYLRPITQAPSETATDASSLFALQAFLRSRDRSHQKFYNMMTKTQMFIRFIEECSFVSDKDASLAFFDDCVDKVDMDKSGEMRLIELDESFKSEHTVFVTPPEIPHLPNGEEPPLQYSYNGFPVLRNNLFERPEGFLQTRKNKLPSKSSSPSSPSPMFRRTKQEIKSAHKIAKRYSSIPQMWSRCLLRHCYGLWFICLPAYVKVCHSKVRALKTAYDVLKKMQSKKMDPPDEVCYRILMQLCGQYDQPVLAVRVLFEMQKAGIDPNAITYGYYNKAVLESTWPSRSRSGYFLWTKVRNVVLGVAQFKRALKKQAHLSQTTLSDGSDLDAVSHGSMDSGHGTHTVEQAPFNTGLIKVYATDDRSSTGGQSDLGYNSLSKDEVRRGDISTEEIQEEKDKKGSDSSSLSESESTKGSADCLPKLNYQSSSSIVRLSGTNNNGADKISGESTESTSKLLLLSSFEGTNETGNSQNRCFRKRHKSDNEPNLQQRLSWGSRNRNLSGGVLMGLMLNRINQEANPGDMVEKLGADAKILSNVISQSTRPTSLDIGKPPLRSKRDSLEKESSDDDTPFDGSNCLADKVDSPVIFDLEDLDSETDGSKVGYIASQNPRRIQRMSSSFSVKPSEKTDVATGFDPLSLLVAETEQQQKEEEEEDDEDSKSVSTPSARRDLAEEIVMYMNNMSSPLTSRTPSIDLQRACDDKSTSKKSPTFVKACRRSSLPPHSPRPVRLTKSKSYTKTEERPRDRLWSSPAFSPTCPFREESQDALTHSSPSFNLDTLLVPKLDVLRNSMFTAGKGVAEKASKWYSRFTMYTTSSKDQSSDRTSLSSVGAQDSESTSLTDEDVCHELEGATSSQESCAASGNKGIDFSRTSLESSASLEGSLSKFALPGKSEVASSCNTSNTNIFQNYAMEVLISSCSRCRTCDCLVHDEEIMAGWTADDSNLNTTCPFCGNLFLPFLNIEIRDLRRPGRYFLKSSPSTENMHFPSSLSSQTRQSCISTSASGLDTSVISVQGNFDLNSKSKLLENTCARTIHIPADRSKTGVSKCPIFPMARSVSTYGPLDKEDTGGQKLIPTGSLPTTLQGATDSLGLEWHLPSPDPITVPYLSPLVVWKELESLLENEGDHAITVADFVDHHPIVFWNLVWYFRRLDLPSNLPGLILSSEHCNKYSKIPRHCMSEDSKYVLIQMLWDNMKLHQDPGQPLYILWNAHSQNRTLLFETQKYPMVHLLQKGDNSFNQELLKNMVKSIKMNDVYGPMSQILETLNKCPHFKRQRSLYREILFLSLVALGRENIDIDAFDKEYKMAYDRLTPSQVKSTHNCDRPPSTGVMECRKTFGEPYL

Target ORF information:

RefSeq Version	XM_005638528.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris DENN/MADD domain containing 4A (DENND4A), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005638528.2

ORF Insert Sequence:

ATGATTGAAG ATAAGGGTCC TCGTGTTGCT GACTACTTTG TTGTAGCAGG ATTAACTGAT 
GTTTCAAAGC CTCTTGAAGA AGAAATTCAC TTCAATGATG CCTGTCATAA AGTAGCTAAA 
CCAAAAGAAC CTATCACAGA TGTTTCGGTG ATTATTAAAT CTCTTGGGGA AGAAGTGCCA 
CGGGATTATA TATGTATTGA TGTTACCCCA ACTGGATTGT CGGCTGATCT CAATAATGGA 
AGTCTTGTGG GGCCACAGAT TTACCTTTGC TACAGAAGAG GAAGAGATAA GCCTCCACTT 
ACAGATCTGG GGGTTTTGTA TGACTGGAAA GAAAGATTGA AACAGGGATG TGAAATTATT 
CAGAGTACTC CCTATGGACA CCCTGCAAAT ATTAGTGGCA GTACCTCATC ACAAAAAATT 
TATATAACTT ACCGAAGAGC CTCTGAAAAC ATGACGCAAA ATACGTTGGC TGTCACAGAC 
ATATGTATTA TTATACCCAG TAAAGGAGAG AGTCCACCAC ACACGTTTTG CAAAGTCGAT 
AAGAATCTCA ATAACAGTAT GTGGGGATCT GCAGTGTACT TATGTTATAA AAAATCAGTG 
GCAAAGACTA ACACTATATC CTATAAAGCT GGTCTAATTT GTAGATATCC TCAAGAAGAT 
TACGAATCTT TCTCACTCCC AGAATCTGTT CCCCTATTTT GTTTACCAAT GGGTGCAACT 
ATTGAATGTT GGCCACCAAA TAGCAAATAC CCTCTCCCTG TATTTTCTAC ATTTGTGTTA 
ACTGGAGCCT CAGCTGAAAA GGTCTATGGT GCTGCTATTC AGTTTTATGA ACCATACTCT 
GAGGAGAATC TCACAGAAAA ACAGAGACTT CTTTTGGGTT TAGCATCATC AGCAGATGGG 
AAATCTGATA GTTCTAAAAC AATTCACACT AGTAAATGCA TCTGTCTTCT TTCTCACTGG 
CCTTTTTTTG ATGCATTCAG GAAATTTTTG ACCTTTCTGT ATCGTTACTC TATCTCTGGA 
CCTCATGTCC TTCCAATTGA GAAGCATATT TCTCATTTTA TGCATAAAGT TCCTTTTCCA 
TCTCCTCAGA GGCCACGGAT TTTAGTTCAG TTATCGCCAC ATGATAATCT GATTCTCAGT 
CAACCTGTTT CTTCACCTCT TCCACTAAGT GGTGGCAAAT TTTCAACACT ACTTCAAAAT 
TTAGGCCCTG AAAATGCTGT AACACTACTG GTGTTTGCAG TAACAGAGCA CAAGATTCTC 
ATCCATTCCT TACGGCCTTC CGTGCTTACT AGCGTGACAG AAGCATTAGT GTCTATGATT 
TTCCCTTTCC ACTGGCCGTG CCCATATGTT CCTCTCTGCC CACTGGCTTT AGCAGATGTC 
TTGAGTGCAC CATGTCCATT CATTGTAGGA ATTGATTCAA GATATTTTGA TCTCTATGAC 
CCTCCACCAG ATGTTAGTTG TGTTGACCTA GATACCAACA CTATTTCTCA AACTGGCGAT 
AAGAAAAATG TAGCCTGGAA GATACTTCCA AAGAAACCAT GTAAAAACCT GATGAACACA 
CTGAATAATT TGCACCAGCA GTTGGCAAAA TTGCAGCAGA GACCAAGAGA TGATGGACTA 
ATGGACTTAG CAATGAATGA CTATGATTTT AATTCTGGAA AGAGGTTGCA TATGATAGAT 
TTGGAAATCC AAGAGGCATT TTTGTGTTTC ATGGCCTCTA TTTTAAAAGG TTATAGATCA 
TACTTAAGAC CAATAACACA AGCCCCTTCT GAGACAGCCA CCGATGCATC CTCTCTTTTT 
GCCCTACAAG CTTTCTTAAG AAGCCGAGAC CGGTCACATC AAAAATTCTA TAACATGATG 
ACCAAAACAC AAATGTTCAT TCGCTTCATT GAGGAATGTT CTTTTGTCAG TGATAAAGAT 
GCAAGCCTGG CATTTTTTGA TGATTGTGTA GATAAAGTGG ATATGGACAA GAGTGGTGAA 
ATGCGACTTA TAGAACTGGA TGAATCTTTC AAAAGTGAAC ATACTGTTTT TGTAACACCA 
CCTGAGATAC CCCATCTACC CAATGGTGAA GAACCCCCTT TACAGTACAG CTATAATGGA 
TTTCCAGTTC TTAGAAACAA TTTATTTGAG AGACCTGAAG GATTTCTACA AACACGAAAG 
AACAAATTGC CTTCAAAATC AAGTAGTCCT AGTAGCCCTT CTCCCATGTT CAGAAGAACA 
AAACAGGAAA TTAAATCAGC CCATAAAATT GCCAAGAGGT ACTCTTCCAT TCCTCAGATG 
TGGTCTAGGT GTCTGCTGCG CCACTGCTAT GGACTGTGGT TCATTTGTCT GCCAGCTTAT 
GTGAAAGTGT GTCATTCAAA AGTCAGGGCA CTGAAGACAG CATATGATGT GCTCAAAAAA 
ATGCAGTCGA AAAAGATGGA TCCACCTGAT GAAGTGTGCT ACCGCATTCT CATGCAGCTC 
TGTGGGCAGT ACGATCAGCC CGTGCTTGCA GTTCGAGTGC TTTTTGAAAT GCAGAAAGCT 
GGTATTGATC CCAATGCCAT TACTTATGGT TATTATAATA AGGCTGTTTT GGAAAGCACC 
TGGCCCTCAA GAAGTCGTAG TGGCTATTTT CTTTGGACAA AAGTAAGAAA CGTTGTTTTA 
GGAGTAGCAC AGTTCAAAAG AGCTTTAAAG AAACAGGCAC ACTTATCACA AACAACTCTC 
TCAGATGGCA GTGACCTGGA TGCTGTTAGT CATGGCAGCA TGGATAGTGG TCATGGGACA 
CACACTGTGG AGCAGGCACC TTTTAATACG GGTTTAATCA AAGTATATGC TACTGATGAT 
AGATCTAGTA CAGGTGGCCA GTCTGATCTT GGATATAATT CATTATCTAA GGATGAAGTT 
AGAAGAGGGG ATATATCTAC TGAGGAAATT CAAGAAGAAA AAGATAAGAA AGGAAGTGAT 
TCTAGTTCCC TGTCAGAGAG TGAGAGTACA AAAGGAAGTG CTGACTGTCT TCCTAAACTC 
AATTATCAAA GTTCTTCCAG TATAGTTCGC CTCAGTGGTA CAAATAACAA TGGTGCTGAT 
AAAATATCAG GAGAAAGCAC AGAAAGCACA TCTAAGCTGC TCTTACTATC ATCTTTTGAG 
GGTACAAATG AAACAGGAAA TAGTCAAAAT AGATGCTTCA GGAAAAGACA TAAAAGTGAC 
AACGAACCTA ATTTGCAACA GCGACTCTCC TGGGGAAGCA GAAACCGCAA TCTTAGTGGA 
GGAGTGCTGA TGGGACTTAT GCTTAATAGA ATCAACCAGG AAGCAAACCC TGGAGACATG 
GTTGAAAAAC TAGGAGCTGA TGCAAAAATT CTTTCAAACG TTATATCACA AAGCACAAGA 
CCAACTAGTC TTGATATTGG AAAACCCCCT TTAAGATCAA AAAGAGACAG TCTAGAAAAG 
GAATCTAGCG ACGATGATAC ACCTTTCGAT GGTTCTAATT GTTTGGCAGA TAAAGTGGAT 
TCTCCTGTTA TTTTTGATTT GGAAGACTTA GACAGTGAGA CAGATGGATC AAAAGTGGGA 
TACATTGCTT CACAAAATCC CAGAAGGATT CAGCGTATGA GCAGCAGCTT CTCGGTAAAA 
CCTTCTGAAA AAACGGATGT TGCCACAGGA TTTGATCCCC TCTCTCTTTT GGTTGCTGAG 
ACTGAACAGC AGCAAAAAGA GGAAGAGGAG GAGGATGATG AAGATAGCAA AAGCGTTTCA 
ACACCATCCG CCAGGCGTGA CTTAGCCGAA GAGATTGTGA TGTATATGAA TAACATGAGC 
AGTCCTTTGA CAAGCCGCAC ACCAAGCATC GATTTACAAC GGGCATGTGA TGACAAATCA 
ACCAGTAAGA AAAGTCCAAC ATTTGTCAAG GCATGCAGGA GATCTAGCCT GCCTCCTCAT 
TCTCCGAGGC CAGTGAGACT TACCAAATCT AAAAGTTATA CAAAAACTGA GGAGAGACCT 
CGGGACAGAC TCTGGTCTTC TCCAGCCTTC TCACCTACTT GCCCATTTAG GGAAGAATCT 
CAAGACGCAT TAACCCATTC ATCACCTTCA TTTAATTTAG ATACACTGCT AGTACCTAAA 
CTAGATGTTC TAAGGAACAG TATGTTCACT GCTGGGAAAG GAGTTGCCGA GAAAGCCAGC 
AAATGGTATT CAAGATTCAC CATGTATACC ACATCATCTA AGGATCAAAG TTCTGATCGT 
ACCAGTCTTT CTTCAGTGGG AGCCCAGGAT TCTGAATCTA CCTCTTTAAC AGATGAAGAT 
GTTTGCCATG AATTGGAAGG AGCCACTTCC TCCCAAGAGA GCTGTGCTGC TTCAGGGAAT 
AAGGGAATTG ATTTCAGCAG AACAAGCCTG GAAAGTTCTG CCTCCTTAGA AGGATCCCTG 
TCAAAGTTTG CCTTACCTGG GAAATCAGAA GTGGCATCTT CCTGCAACAC AAGTAATACA 
AATATCTTCC AGAACTATGC AATGGAGGTT CTTATCTCAA GTTGCTCTCG GTGTAGAACT 
TGTGATTGTC TTGTCCATGA TGAGGAAATC ATGGCTGGCT GGACAGCAGA TGATTCAAAT 
CTCAATACTA CATGCCCGTT TTGTGGCAAT CTCTTCTTGC CCTTTCTGAA TATTGAAATA 
AGAGATTTAA GACGACCCGG AAGATACTTT CTGAAGTCAA GTCCATCTAC AGAAAATATG 
CATTTTCCAT CCTCCCTTTC AAGTCAGACA AGGCAGTCTT GTATCTCAAC ATCAGCCTCT 
GGTCTTGACA CGTCTGTTAT CTCTGTTCAA GGGAATTTTG ATCTCAATAG CAAATCTAAA 
CTGCTGGAAA ATACATGTGC CCGAACTATT CACATCCCTG CTGATAGATC AAAAACAGGT 
GTGTCAAAAT GTCCAATATT TCCAATGGCC AGGAGTGTTA GTACTTATGG CCCCTTGGAT 
AAGGAAGACA CTGGAGGCCA GAAGCTCATT CCTACAGGCA GCCTGCCAAC TACTTTACAA 
GGAGCTACAG ATTCTTTAGG ATTAGAGTGG CACCTTCCTA GTCCAGATCC TATCACAGTT 
CCATATCTTA GTCCTTTAGT GGTATGGAAG GAGCTTGAAA GCTTACTGGA AAATGAAGGC 
GACCATGCAA TAACAGTGGC AGACTTTGTG GATCATCATC CAATCGTGTT TTGGAACCTG 
GTGTGGTATT TTAGACGTCT CGACTTACCC AGTAACTTAC CTGGATTGAT TCTTTCTTCT 
GAGCACTGTA ACAAGTATTC AAAGATTCCT CGCCACTGTA TGTCGGAAGA TAGTAAATAC 
GTTTTAATAC AAATGTTATG GGACAATATG AAATTACATC AGGATCCGGG ACAGCCTTTG 
TACATCCTCT GGAATGCCCA CAGTCAAAAT CGTACTCTTT TGTTTGAGAC CCAAAAGTAT 
CCAATGGTCC ATTTATTGCA AAAGGGTGAT AATTCATTTA ACCAGGAACT GTTGAAAAAT 
ATGGTAAAAA GCATTAAAAT GAATGATGTC TATGGACCAA TGAGTCAAAT TTTAGAGACA 
CTGAATAAAT GTCCTCATTT TAAAAGACAG AGGAGTTTAT ATAGAGAAAT ACTATTTCTC 
TCACTTGTGG CACTGGGAAG AGAAAACATT GATATAGATG CATTTGATAA AGAATACAAG 
ATGGCATACG ATCGTCTCAC ACCTAGTCAA GTCAAGAGTA CACACAACTG TGATAGACCA 
CCAAGTACAG GGGTGATGGA ATGTCGAAAA ACCTTTGGAG AACCTTATCT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DENND4A ( XM_005638528.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005638585.1 Canis lupus familiaris DENN domain containing 4A (DENND4A), transcript variant X4, mRNA.

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CloneID	OCe29019
Clone ID Related Accession (Same CDS sequence)	XM_005638528.2 , XM_005638528.3
Accession Version	XM_005638528.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5754bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	C-myc promoter-binding protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006612.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005638528.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTGAAG ATAAGGGTCC TCGTGTTGCT GACTACTTTG TTGTAGCAGG ATTAACTGAT 
GTTTCAAAGC CTCTTGAAGA AGAAATTCAC TTCAATGATG CCTGTCATAA AGTAGCTAAA 
CCAAAAGAAC CTATCACAGA TGTTTCGGTG ATTATTAAAT CTCTTGGGGA AGAAGTGCCA 
CGGGATTATA TATGTATTGA TGTTACCCCA ACTGGATTGT CGGCTGATCT CAATAATGGA 
AGTCTTGTGG GGCCACAGAT TTACCTTTGC TACAGAAGAG GAAGAGATAA GCCTCCACTT 
ACAGATCTGG GGGTTTTGTA TGACTGGAAA GAAAGATTGA AACAGGGATG TGAAATTATT 
CAGAGTACTC CCTATGGACA CCCTGCAAAT ATTAGTGGCA GTACCTCATC ACAAAAAATT 
TATATAACTT ACCGAAGAGC CTCTGAAAAC ATGACGCAAA ATACGTTGGC TGTCACAGAC 
ATATGTATTA TTATACCCAG TAAAGGAGAG AGTCCACCAC ACACGTTTTG CAAAGTCGAT 
AAGAATCTCA ATAACAGTAT GTGGGGATCT GCAGTGTACT TATGTTATAA AAAATCAGTG 
GCAAAGACTA ACACTATATC CTATAAAGCT GGTCTAATTT GTAGATATCC TCAAGAAGAT 
TACGAATCTT TCTCACTCCC AGAATCTGTT CCCCTATTTT GTTTACCAAT GGGTGCAACT 
ATTGAATGTT GGCCACCAAA TAGCAAATAC CCTCTCCCTG TATTTTCTAC ATTTGTGTTA 
ACTGGAGCCT CAGCTGAAAA GGTCTATGGT GCTGCTATTC AGTTTTATGA ACCATACTCT 
GAGGAGAATC TCACAGAAAA ACAGAGACTT CTTTTGGGTT TAGCATCATC AGCAGATGGG 
AAATCTGATA GTTCTAAAAC AATTCACACT AGTAAATGCA TCTGTCTTCT TTCTCACTGG 
CCTTTTTTTG ATGCATTCAG GAAATTTTTG ACCTTTCTGT ATCGTTACTC TATCTCTGGA 
CCTCATGTCC TTCCAATTGA GAAGCATATT TCTCATTTTA TGCATAAAGT TCCTTTTCCA 
TCTCCTCAGA GGCCACGGAT TTTAGTTCAG TTATCGCCAC ATGATAATCT GATTCTCAGT 
CAACCTGTTT CTTCACCTCT TCCACTAAGT GGTGGCAAAT TTTCAACACT ACTTCAAAAT 
TTAGGCCCTG AAAATGCTGT AACACTACTG GTGTTTGCAG TAACAGAGCA CAAGATTCTC 
ATCCATTCCT TACGGCCTTC CGTGCTTACT AGCGTGACAG AAGCATTAGT GTCTATGATT 
TTCCCTTTCC ACTGGCCGTG CCCATATGTT CCTCTCTGCC CACTGGCTTT AGCAGATGTC 
TTGAGTGCAC CATGTCCATT CATTGTAGGA ATTGATTCAA GATATTTTGA TCTCTATGAC 
CCTCCACCAG ATGTTAGTTG TGTTGACCTA GATACCAACA CTATTTCTCA AACTGGCGAT 
AAGAAAAATG TAGCCTGGAA GATACTTCCA AAGAAACCAT GTAAAAACCT GATGAACACA 
CTGAATAATT TGCACCAGCA GTTGGCAAAA TTGCAGCAGA GACCAAGAGA TGATGGACTA 
ATGGACTTAG CAATGAATGA CTATGATTTT AATTCTGGAA AGAGGTTGCA TATGATAGAT 
TTGGAAATCC AAGAGGCATT TTTGTGTTTC ATGGCCTCTA TTTTAAAAGG TTATAGATCA 
TACTTAAGAC CAATAACACA AGCCCCTTCT GAGACAGCCA CCGATGCATC CTCTCTTTTT 
GCCCTACAAG CTTTCTTAAG AAGCCGAGAC CGGTCACATC AAAAATTCTA TAACATGATG 
ACCAAAACAC AAATGTTCAT TCGCTTCATT GAGGAATGTT CTTTTGTCAG TGATAAAGAT 
GCAAGCCTGG CATTTTTTGA TGATTGTGTA GATAAAGTGG ATATGGACAA GAGTGGTGAA 
ATGCGACTTA TAGAACTGGA TGAATCTTTC AAAAGTGAAC ATACTGTTTT TGTAACACCA 
CCTGAGATAC CCCATCTACC CAATGGTGAA GAACCCCCTT TACAGTACAG CTATAATGGA 
TTTCCAGTTC TTAGAAACAA TTTATTTGAG AGACCTGAAG GATTTCTACA AACACGAAAG 
AACAAATTGC CTTCAAAATC AAGTAGTCCT AGTAGCCCTT CTCCCATGTT CAGAAGAACA 
AAACAGGAAA TTAAATCAGC CCATAAAATT GCCAAGAGGT ACTCTTCCAT TCCTCAGATG 
TGGTCTAGGT GTCTGCTGCG CCACTGCTAT GGACTGTGGT TCATTTGTCT GCCAGCTTAT 
GTGAAAGTGT GTCATTCAAA AGTCAGGGCA CTGAAGACAG CATATGATGT GCTCAAAAAA 
ATGCAGTCGA AAAAGATGGA TCCACCTGAT GAAGTGTGCT ACCGCATTCT CATGCAGCTC 
TGTGGGCAGT ACGATCAGCC CGTGCTTGCA GTTCGAGTGC TTTTTGAAAT GCAGAAAGCT 
GGTATTGATC CCAATGCCAT TACTTATGGT TATTATAATA AGGCTGTTTT GGAAAGCACC 
TGGCCCTCAA GAAGTCGTAG TGGCTATTTT CTTTGGACAA AAGTAAGAAA CGTTGTTTTA 
GGAGTAGCAC AGTTCAAAAG AGCTTTAAAG AAACAGGCAC ACTTATCACA AACAACTCTC 
TCAGATGGCA GTGACCTGGA TGCTGTTAGT CATGGCAGCA TGGATAGTGG TCATGGGACA 
CACACTGTGG AGCAGGCACC TTTTAATACG GGTTTAATCA AAGTATATGC TACTGATGAT 
AGATCTAGTA CAGGTGGCCA GTCTGATCTT GGATATAATT CATTATCTAA GGATGAAGTT 
AGAAGAGGGG ATATATCTAC TGAGGAAATT CAAGAAGAAA AAGATAAGAA AGGAAGTGAT 
TCTAGTTCCC TGTCAGAGAG TGAGAGTACA AAAGGAAGTG CTGACTGTCT TCCTAAACTC 
AATTATCAAA GTTCTTCCAG TATAGTTCGC CTCAGTGGTA CAAATAACAA TGGTGCTGAT 
AAAATATCAG GAGAAAGCAC AGAAAGCACA TCTAAGCTGC TCTTACTATC ATCTTTTGAG 
GGTACAAATG AAACAGGAAA TAGTCAAAAT AGATGCTTCA GGAAAAGACA TAAAAGTGAC 
AACGAACCTA ATTTGCAACA GCGACTCTCC TGGGGAAGCA GAAACCGCAA TCTTAGTGGA 
GGAGTGCTGA TGGGACTTAT GCTTAATAGA ATCAACCAGG AAGCAAACCC TGGAGACATG 
GTTGAAAAAC TAGGAGCTGA TGCAAAAATT CTTTCAAACG TTATATCACA AAGCACAAGA 
CCAACTAGTC TTGATATTGG AAAACCCCCT TTAAGATCAA AAAGAGACAG TCTAGAAAAG 
GAATCTAGCG ACGATGATAC ACCTTTCGAT GGTTCTAATT GTTTGGCAGA TAAAGTGGAT 
TCTCCTGTTA TTTTTGATTT GGAAGACTTA GACAGTGAGA CAGATGGATC AAAAGTGGGA 
TACATTGCTT CACAAAATCC CAGAAGGATT CAGCGTATGA GCAGCAGCTT CTCGGTAAAA 
CCTTCTGAAA AAACGGATGT TGCCACAGGA TTTGATCCCC TCTCTCTTTT GGTTGCTGAG 
ACTGAACAGC AGCAAAAAGA GGAAGAGGAG GAGGATGATG AAGATAGCAA AAGCGTTTCA 
ACACCATCCG CCAGGCGTGA CTTAGCCGAA GAGATTGTGA TGTATATGAA TAACATGAGC 
AGTCCTTTGA CAAGCCGCAC ACCAAGCATC GATTTACAAC GGGCATGTGA TGACAAATCA 
ACCAGTAAGA AAAGTCCAAC ATTTGTCAAG GCATGCAGGA GATCTAGCCT GCCTCCTCAT 
TCTCCGAGGC CAGTGAGACT TACCAAATCT AAAAGTTATA CAAAAACTGA GGAGAGACCT 
CGGGACAGAC TCTGGTCTTC TCCAGCCTTC TCACCTACTT GCCCATTTAG GGAAGAATCT 
CAAGACGCAT TAACCCATTC ATCACCTTCA TTTAATTTAG ATACACTGCT AGTACCTAAA 
CTAGATGTTC TAAGGAACAG TATGTTCACT GCTGGGAAAG GAGTTGCCGA GAAAGCCAGC 
AAATGGTATT CAAGATTCAC CATGTATACC ACATCATCTA AGGATCAAAG TTCTGATCGT 
ACCAGTCTTT CTTCAGTGGG AGCCCAGGAT TCTGAATCTA CCTCTTTAAC AGATGAAGAT 
GTTTGCCATG AATTGGAAGG AGCCACTTCC TCCCAAGAGA GCTGTGCTGC TTCAGGGAAT 
AAGGGAATTG ATTTCAGCAG AACAAGCCTG GAAAGTTCTG CCTCCTTAGA AGGATCCCTG 
TCAAAGTTTG CCTTACCTGG GAAATCAGAA GTGGCATCTT CCTGCAACAC AAGTAATACA 
AATATCTTCC AGAACTATGC AATGGAGGTT CTTATCTCAA GTTGCTCTCG GTGTAGAACT 
TGTGATTGTC TTGTCCATGA TGAGGAAATC ATGGCTGGCT GGACAGCAGA TGATTCAAAT 
CTCAATACTA CATGCCCGTT TTGTGGCAAT CTCTTCTTGC CCTTTCTGAA TATTGAAATA 
AGAGATTTAA GACGACCCGG AAGATACTTT CTGAAGTCAA GTCCATCTAC AGAAAATATG 
CATTTTCCAT CCTCCCTTTC AAGTCAGACA AGGCAGTCTT GTATCTCAAC ATCAGCCTCT 
GGTCTTGACA CGTCTGTTAT CTCTGTTCAA GGGAATTTTG ATCTCAATAG CAAATCTAAA 
CTGCTGGAAA ATACATGTGC CCGAACTATT CACATCCCTG CTGATAGATC AAAAACAGGT 
GTGTCAAAAT GTCCAATATT TCCAATGGCC AGGAGTGTTA GTACTTATGG CCCCTTGGAT 
AAGGAAGACA CTGGAGGCCA GAAGCTCATT CCTACAGGCA GCCTGCCAAC TACTTTACAA 
GGAGCTACAG ATTCTTTAGG ATTAGAGTGG CACCTTCCTA GTCCAGATCC TATCACAGTT 
CCATATCTTA GTCCTTTAGT GGTATGGAAG GAGCTTGAAA GCTTACTGGA AAATGAAGGC 
GACCATGCAA TAACAGTGGC AGACTTTGTG GATCATCATC CAATCGTGTT TTGGAACCTG 
GTGTGGTATT TTAGACGTCT CGACTTACCC AGTAACTTAC CTGGATTGAT TCTTTCTTCT 
GAGCACTGTA ACAAGTATTC AAAGATTCCT CGCCACTGTA TGTCGGAAGA TAGTAAATAC 
GTTTTAATAC AAATGTTATG GGACAATATG AAATTACATC AGGATCCGGG ACAGCCTTTG 
TACATCCTCT GGAATGCCCA CAGTCAAAAT CGTACTCTTT TGTTTGAGAC CCAAAAGTAT 
CCAATGGTCC ATTTATTGCA AAAGGGTGAT AATTCATTTA ACCAGGAACT GTTGAAAAAT 
ATGGTAAAAA GCATTAAAAT GAATGATGTC TATGGACCAA TGAGTCAAAT TTTAGAGACA 
CTGAATAAAT GTCCTCATTT TAAAAGACAG AGGAGTTTAT ATAGAGAAAT ACTATTTCTC 
TCACTTGTGG CACTGGGAAG AGAAAACATT GATATAGATG CATTTGATAA AGAATACAAG 
ATGGCATACG ATCGTCTCAC ACCTAGTCAA GTCAAGAGTA CACACAACTG TGATAGACCA 
CCAAGTACAG GGGTGATGGA ATGTCGAAAA ACCTTTGGAG AACCTTATCT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005638585.1
CDS	344..6097
Translation	MIEDKGPRVADYFVVAGLTDVSKPLEEEIHFNDACHKVAKPKEPITDVSVIIKSLGEEVPRDYICIDVTPTGLSADLNNGSLVGPQIYLCYRRGRDKPPLTDLGVLYDWKERLKQGCEIIQSTPYGHPANISGSTSSQKIYITYRRASENMTQNTLAVTDICIIIPSKGESPPHTFCKVDKNLNNSMWGSAVYLCYKKSVAKTNTISYKAGLICRYPQEDYESFSLPESVPLFCLPMGATIECWPPNSKYPLPVFSTFVLTGASAEKVYGAAIQFYEPYSEENLTEKQRLLLGLASSADGKSDSSKTIHTSKCICLLSHWPFFDAFRKFLTFLYRYSISGPHVLPIEKHISHFMHKVPFPSPQRPRILVQLSPHDNLILSQPVSSPLPLSGGKFSTLLQNLGPENAVTLLVFAVTEHKILIHSLRPSVLTSVTEALVSMIFPFHWPCPYVPLCPLALADVLSAPCPFIVGIDSRYFDLYDPPPDVSCVDLDTNTISQTGDKKNVAWKILPKKPCKNLMNTLNNLHQQLAKLQQRPRDDGLMDLAMNDYDFNSGKRLHMIDLEIQEAFLCFMASILKGYRSYLRPITQAPSETATDASSLFALQAFLRSRDRSHQKFYNMMTKTQMFIRFIEECSFVSDKDASLAFFDDCVDKVDMDKSGEMRLIELDESFKSEHTVFVTPPEIPHLPNGEEPPLQYSYNGFPVLRNNLFERPEGFLQTRKNKLPSKSSSPSSPSPMFRRTKQEIKSAHKIAKRYSSIPQMWSRCLLRHCYGLWFICLPAYVKVCHSKVRALKTAYDVLKKMQSKKMDPPDEVCYRILMQLCGQYDQPVLAVRVLFEMQKAGIDPNAITYGYYNKAVLESTWPSRSRSGYFLWTKVRNVVLGVAQFKRALKKQAHLSQTTLSDGSDLDAVSHGSMDSGHGTHTVEQAPFNTGLIKVYATDDRSSTGGQSDLGYNSLSKDEVRRGDISTEEIQEEKDKKGSDSSSLSESESTKGSADCLPKLNYQSSSSIVRLSGTNNNGADKISGESTESTSKLLLLSSFEGTNETGNSQNRCFRKRHKSDNEPNLQQRLSWGSRNRNLSGGVLMGLMLNRINQEANPGDMVEKLGADAKILSNVISQSTRPTSLDIGKPPLRSKRDSLEKESSDDDTPFDGSNCLADKVDSPVIFDLEDLDSETDGSKVGYIASQNPRRIQRMSSSFSVKPSEKTDVATGFDPLSLLVAETEQQQKEEEEEDDEDSKSVSTPSARRDLAEEIVMYMNNMSSPLTSRTPSIDLQRACDDKSTSKKSPTFVKACRRSSLPPHSPRPVRLTKSKSYTKTEERPRDRLWSSPAFSPTCPFREESQDALTHSSPSFNLDTLLVPKLDVLRNSMFTAGKGVAEKASKWYSRFTMYTTSSKDQSSDRTSLSSVGAQDSESTSLTDEDVCHELEGATSSQESCAASGNKGIDFSRTSLESSASLEGSLSKFALPGKSEVASSCNTSNTNIFQNYAMEVLISSCSRCRTCDCLVHDEEIMAGWTADDSNLNTTCPFCGNLFLPFLNIEIRDLRRPGRYFLKSSPSTENMHFPSSLSSQTRQSCISTSASGLDTSVISVQGNFDLNSKSKLLENTCARTIHIPADRSKTGVSKCPIFPMARSVSTYGPLDKEDTGGQKLIPTGSLPTTLQGATDSLGLEWHLPSPDPITVPYLSPLVVWKELESLLENEGDHAITVADFVDHHPIVFWNLVWYFRRLDLPSNLPGLILSSEHCNKYSKIPRHCMSEDSKYVLIQMLWDNMKLHQDPGQPLYILWNAHSQNRTLLFETQKYPMVHLLQKGDNSFNQELLKNMVKSIKMNDVYGPMSQILETLNKCPHFKRQRSLYREILFLSLVALGRENIDIDAFDKEYKMAYDRLTPSQVKSTHNCDRPPSTGVMECRKTFGEPYL

Target ORF information:

RefSeq Version	XM_005638528.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris DENN domain containing 4A (DENND4A), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005638528.3

ORF Insert Sequence:

ATGATTGAAG ATAAGGGTCC TCGTGTTGCT GACTACTTTG TTGTAGCAGG ATTAACTGAT 
GTTTCAAAGC CTCTTGAAGA AGAAATTCAC TTCAATGATG CCTGTCATAA AGTAGCTAAA 
CCAAAAGAAC CTATCACAGA TGTTTCGGTG ATTATTAAAT CTCTTGGGGA AGAAGTGCCA 
CGGGATTATA TATGTATTGA TGTTACCCCA ACTGGATTGT CGGCTGATCT CAATAATGGA 
AGTCTTGTGG GGCCACAGAT TTACCTTTGC TACAGAAGAG GAAGAGATAA GCCTCCACTT 
ACAGATCTGG GGGTTTTGTA TGACTGGAAA GAAAGATTGA AACAGGGATG TGAAATTATT 
CAGAGTACTC CCTATGGACA CCCTGCAAAT ATTAGTGGCA GTACCTCATC ACAAAAAATT 
TATATAACTT ACCGAAGAGC CTCTGAAAAC ATGACGCAAA ATACGTTGGC TGTCACAGAC 
ATATGTATTA TTATACCCAG TAAAGGAGAG AGTCCACCAC ACACGTTTTG CAAAGTCGAT 
AAGAATCTCA ATAACAGTAT GTGGGGATCT GCAGTGTACT TATGTTATAA AAAATCAGTG 
GCAAAGACTA ACACTATATC CTATAAAGCT GGTCTAATTT GTAGATATCC TCAAGAAGAT 
TACGAATCTT TCTCACTCCC AGAATCTGTT CCCCTATTTT GTTTACCAAT GGGTGCAACT 
ATTGAATGTT GGCCACCAAA TAGCAAATAC CCTCTCCCTG TATTTTCTAC ATTTGTGTTA 
ACTGGAGCCT CAGCTGAAAA GGTCTATGGT GCTGCTATTC AGTTTTATGA ACCATACTCT 
GAGGAGAATC TCACAGAAAA ACAGAGACTT CTTTTGGGTT TAGCATCATC AGCAGATGGG 
AAATCTGATA GTTCTAAAAC AATTCACACT AGTAAATGCA TCTGTCTTCT TTCTCACTGG 
CCTTTTTTTG ATGCATTCAG GAAATTTTTG ACCTTTCTGT ATCGTTACTC TATCTCTGGA 
CCTCATGTCC TTCCAATTGA GAAGCATATT TCTCATTTTA TGCATAAAGT TCCTTTTCCA 
TCTCCTCAGA GGCCACGGAT TTTAGTTCAG TTATCGCCAC ATGATAATCT GATTCTCAGT 
CAACCTGTTT CTTCACCTCT TCCACTAAGT GGTGGCAAAT TTTCAACACT ACTTCAAAAT 
TTAGGCCCTG AAAATGCTGT AACACTACTG GTGTTTGCAG TAACAGAGCA CAAGATTCTC 
ATCCATTCCT TACGGCCTTC CGTGCTTACT AGCGTGACAG AAGCATTAGT GTCTATGATT 
TTCCCTTTCC ACTGGCCGTG CCCATATGTT CCTCTCTGCC CACTGGCTTT AGCAGATGTC 
TTGAGTGCAC CATGTCCATT CATTGTAGGA ATTGATTCAA GATATTTTGA TCTCTATGAC 
CCTCCACCAG ATGTTAGTTG TGTTGACCTA GATACCAACA CTATTTCTCA AACTGGCGAT 
AAGAAAAATG TAGCCTGGAA GATACTTCCA AAGAAACCAT GTAAAAACCT GATGAACACA 
CTGAATAATT TGCACCAGCA GTTGGCAAAA TTGCAGCAGA GACCAAGAGA TGATGGACTA 
ATGGACTTAG CAATGAATGA CTATGATTTT AATTCTGGAA AGAGGTTGCA TATGATAGAT 
TTGGAAATCC AAGAGGCATT TTTGTGTTTC ATGGCCTCTA TTTTAAAAGG TTATAGATCA 
TACTTAAGAC CAATAACACA AGCCCCTTCT GAGACAGCCA CCGATGCATC CTCTCTTTTT 
GCCCTACAAG CTTTCTTAAG AAGCCGAGAC CGGTCACATC AAAAATTCTA TAACATGATG 
ACCAAAACAC AAATGTTCAT TCGCTTCATT GAGGAATGTT CTTTTGTCAG TGATAAAGAT 
GCAAGCCTGG CATTTTTTGA TGATTGTGTA GATAAAGTGG ATATGGACAA GAGTGGTGAA 
ATGCGACTTA TAGAACTGGA TGAATCTTTC AAAAGTGAAC ATACTGTTTT TGTAACACCA 
CCTGAGATAC CCCATCTACC CAATGGTGAA GAACCCCCTT TACAGTACAG CTATAATGGA 
TTTCCAGTTC TTAGAAACAA TTTATTTGAG AGACCTGAAG GATTTCTACA AACACGAAAG 
AACAAATTGC CTTCAAAATC AAGTAGTCCT AGTAGCCCTT CTCCCATGTT CAGAAGAACA 
AAACAGGAAA TTAAATCAGC CCATAAAATT GCCAAGAGGT ACTCTTCCAT TCCTCAGATG 
TGGTCTAGGT GTCTGCTGCG CCACTGCTAT GGACTGTGGT TCATTTGTCT GCCAGCTTAT 
GTGAAAGTGT GTCATTCAAA AGTCAGGGCA CTGAAGACAG CATATGATGT GCTCAAAAAA 
ATGCAGTCGA AAAAGATGGA TCCACCTGAT GAAGTGTGCT ACCGCATTCT CATGCAGCTC 
TGTGGGCAGT ACGATCAGCC CGTGCTTGCA GTTCGAGTGC TTTTTGAAAT GCAGAAAGCT 
GGTATTGATC CCAATGCCAT TACTTATGGT TATTATAATA AGGCTGTTTT GGAAAGCACC 
TGGCCCTCAA GAAGTCGTAG TGGCTATTTT CTTTGGACAA AAGTAAGAAA CGTTGTTTTA 
GGAGTAGCAC AGTTCAAAAG AGCTTTAAAG AAACAGGCAC ACTTATCACA AACAACTCTC 
TCAGATGGCA GTGACCTGGA TGCTGTTAGT CATGGCAGCA TGGATAGTGG TCATGGGACA 
CACACTGTGG AGCAGGCACC TTTTAATACG GGTTTAATCA AAGTATATGC TACTGATGAT 
AGATCTAGTA CAGGTGGCCA GTCTGATCTT GGATATAATT CATTATCTAA GGATGAAGTT 
AGAAGAGGGG ATATATCTAC TGAGGAAATT CAAGAAGAAA AAGATAAGAA AGGAAGTGAT 
TCTAGTTCCC TGTCAGAGAG TGAGAGTACA AAAGGAAGTG CTGACTGTCT TCCTAAACTC 
AATTATCAAA GTTCTTCCAG TATAGTTCGC CTCAGTGGTA CAAATAACAA TGGTGCTGAT 
AAAATATCAG GAGAAAGCAC AGAAAGCACA TCTAAGCTGC TCTTACTATC ATCTTTTGAG 
GGTACAAATG AAACAGGAAA TAGTCAAAAT AGATGCTTCA GGAAAAGACA TAAAAGTGAC 
AACGAACCTA ATTTGCAACA GCGACTCTCC TGGGGAAGCA GAAACCGCAA TCTTAGTGGA 
GGAGTGCTGA TGGGACTTAT GCTTAATAGA ATCAACCAGG AAGCAAACCC TGGAGACATG 
GTTGAAAAAC TAGGAGCTGA TGCAAAAATT CTTTCAAACG TTATATCACA AAGCACAAGA 
CCAACTAGTC TTGATATTGG AAAACCCCCT TTAAGATCAA AAAGAGACAG TCTAGAAAAG 
GAATCTAGCG ACGATGATAC ACCTTTCGAT GGTTCTAATT GTTTGGCAGA TAAAGTGGAT 
TCTCCTGTTA TTTTTGATTT GGAAGACTTA GACAGTGAGA CAGATGGATC AAAAGTGGGA 
TACATTGCTT CACAAAATCC CAGAAGGATT CAGCGTATGA GCAGCAGCTT CTCGGTAAAA 
CCTTCTGAAA AAACGGATGT TGCCACAGGA TTTGATCCCC TCTCTCTTTT GGTTGCTGAG 
ACTGAACAGC AGCAAAAAGA GGAAGAGGAG GAGGATGATG AAGATAGCAA AAGCGTTTCA 
ACACCATCCG CCAGGCGTGA CTTAGCCGAA GAGATTGTGA TGTATATGAA TAACATGAGC 
AGTCCTTTGA CAAGCCGCAC ACCAAGCATC GATTTACAAC GGGCATGTGA TGACAAATCA 
ACCAGTAAGA AAAGTCCAAC ATTTGTCAAG GCATGCAGGA GATCTAGCCT GCCTCCTCAT 
TCTCCGAGGC CAGTGAGACT TACCAAATCT AAAAGTTATA CAAAAACTGA GGAGAGACCT 
CGGGACAGAC TCTGGTCTTC TCCAGCCTTC TCACCTACTT GCCCATTTAG GGAAGAATCT 
CAAGACGCAT TAACCCATTC ATCACCTTCA TTTAATTTAG ATACACTGCT AGTACCTAAA 
CTAGATGTTC TAAGGAACAG TATGTTCACT GCTGGGAAAG GAGTTGCCGA GAAAGCCAGC 
AAATGGTATT CAAGATTCAC CATGTATACC ACATCATCTA AGGATCAAAG TTCTGATCGT 
ACCAGTCTTT CTTCAGTGGG AGCCCAGGAT TCTGAATCTA CCTCTTTAAC AGATGAAGAT 
GTTTGCCATG AATTGGAAGG AGCCACTTCC TCCCAAGAGA GCTGTGCTGC TTCAGGGAAT 
AAGGGAATTG ATTTCAGCAG AACAAGCCTG GAAAGTTCTG CCTCCTTAGA AGGATCCCTG 
TCAAAGTTTG CCTTACCTGG GAAATCAGAA GTGGCATCTT CCTGCAACAC AAGTAATACA 
AATATCTTCC AGAACTATGC AATGGAGGTT CTTATCTCAA GTTGCTCTCG GTGTAGAACT 
TGTGATTGTC TTGTCCATGA TGAGGAAATC ATGGCTGGCT GGACAGCAGA TGATTCAAAT 
CTCAATACTA CATGCCCGTT TTGTGGCAAT CTCTTCTTGC CCTTTCTGAA TATTGAAATA 
AGAGATTTAA GACGACCCGG AAGATACTTT CTGAAGTCAA GTCCATCTAC AGAAAATATG 
CATTTTCCAT CCTCCCTTTC AAGTCAGACA AGGCAGTCTT GTATCTCAAC ATCAGCCTCT 
GGTCTTGACA CGTCTGTTAT CTCTGTTCAA GGGAATTTTG ATCTCAATAG CAAATCTAAA 
CTGCTGGAAA ATACATGTGC CCGAACTATT CACATCCCTG CTGATAGATC AAAAACAGGT 
GTGTCAAAAT GTCCAATATT TCCAATGGCC AGGAGTGTTA GTACTTATGG CCCCTTGGAT 
AAGGAAGACA CTGGAGGCCA GAAGCTCATT CCTACAGGCA GCCTGCCAAC TACTTTACAA 
GGAGCTACAG ATTCTTTAGG ATTAGAGTGG CACCTTCCTA GTCCAGATCC TATCACAGTT 
CCATATCTTA GTCCTTTAGT GGTATGGAAG GAGCTTGAAA GCTTACTGGA AAATGAAGGC 
GACCATGCAA TAACAGTGGC AGACTTTGTG GATCATCATC CAATCGTGTT TTGGAACCTG 
GTGTGGTATT TTAGACGTCT CGACTTACCC AGTAACTTAC CTGGATTGAT TCTTTCTTCT 
GAGCACTGTA ACAAGTATTC AAAGATTCCT CGCCACTGTA TGTCGGAAGA TAGTAAATAC 
GTTTTAATAC AAATGTTATG GGACAATATG AAATTACATC AGGATCCGGG ACAGCCTTTG 
TACATCCTCT GGAATGCCCA CAGTCAAAAT CGTACTCTTT TGTTTGAGAC CCAAAAGTAT 
CCAATGGTCC ATTTATTGCA AAAGGGTGAT AATTCATTTA ACCAGGAACT GTTGAAAAAT 
ATGGTAAAAA GCATTAAAAT GAATGATGTC TATGGACCAA TGAGTCAAAT TTTAGAGACA 
CTGAATAAAT GTCCTCATTT TAAAAGACAG AGGAGTTTAT ATAGAGAAAT ACTATTTCTC 
TCACTTGTGG CACTGGGAAG AGAAAACATT GATATAGATG CATTTGATAA AGAATACAAG 
ATGGCATACG ATCGTCTCAC ACCTAGTCAA GTCAAGAGTA CACACAACTG TGATAGACCA 
CCAAGTACAG GGGTGATGGA ATGTCGAAAA ACCTTTGGAG AACCTTATCT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DENND4A ( XM_005638529.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005638586.1 Canis lupus familiaris DENN domain containing 4A (DENND4A), transcript variant X5, mRNA.

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CloneID	OCe29020
Clone ID Related Accession (Same CDS sequence)	XM_005638529.3 , XM_005638529.2
Accession Version	XM_005638529.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5730bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	C-myc promoter-binding protein isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006612.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005638529.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTGAAG ATAAGGGTCC TCGTGTTGCT GACTACTTTG TTGTAGCAGG ATTAACTGAT 
GTTTCAAAGC CTCTTGAAGA AGAAATTCAC TTCAATGATG CCTGTCATAA AGTAGCTAAA 
CCAAAAGAAC CTATCACAGA TGTTTCGGTG ATTATTAAAT CTCTTGGGGA AGAAGTGCCA 
CGGGATTATA TATGTATTGA TGTTACCCCA ACTGGATTGT CGGCTGATCT CAATAATGGA 
AGTCTTGTGG GGCCACAGAT TTACCTTTGC TACAGAAGAG GAAGAGATAA GCCTCCACTT 
ACAGATCTGG GGGTTTTGTA TGACTGGAAA GAAAGATTGA AACAGGGATG TGAAATTATT 
CAGAGTACTC CCTATGGACA CCCTGCAAAT ATTAGTGGCA GTACCTCATC ACAAAAAATT 
TATATAACTT ACCGAAGAGC CTCTGAAAAC ATGACGCAAA ATACGTTGGC TGTCACAGAC 
ATATGTATTA TTATACCCAG TAAAGGAGAG AGTCCACCAC ACACGTTTTG CAAAGTCGAT 
AAGAATCTCA ATAACAGTAT GTGGGGATCT GCAGTGTACT TATGTTATAA AAAATCAGTG 
GCAAAGACTA ACACTATATC CTATAAAGCT GGTCTAATTT GTAGATATCC TCAAGAAGAT 
TACGAATCTT TCTCACTCCC AGAATCTGTT CCCCTATTTT GTTTACCAAT GGGTGCAACT 
ATTGAATGTT GGCCACCAAA TAGCAAATAC CCTCTCCCTG TATTTTCTAC ATTTGTGTTA 
ACTGGAGCCT CAGCTGAAAA GGTCTATGGT GCTGCTATTC AGTTTTATGA ACCATACTCT 
GAGGAGAATC TCACAGAAAA ACAGAGACTT CTTTTGGGTT TAGCATCATC AGCAGATGGG 
AAATCTGATA GTTCTAAAAC AATTCACACT AGTAAATGCA TCTGTCTTCT TTCTCACTGG 
CCTTTTTTTG ATGCATTCAG GAAATTTTTG ACCTTTCTGT ATCGTTACTC TATCTCTGGA 
CCTCATGTCC TTCCAATTGA GAAGCATATT TCTCATTTTA TGCATAAAGT TCCTTTTCCA 
TCTCCTCAGA GGCCACGGAT TTTAGTTCAG TTATCGCCAC ATGATAATCT GATTCTCAGT 
CAACCTGTTT CTTCACCTCT TCCACTAAGT GGTGGCAAAT TTTCAACACT ACTTCAAAAT 
TTAGGCCCTG AAAATGCTGT AACACTACTG GTGTTTGCAG TAACAGAGCA CAAGATTCTC 
ATCCATTCCT TACGGCCTTC CGTGCTTACT AGCGTGACAG AAGCATTAGT GTCTATGATT 
TTCCCTTTCC ACTGGCCGTG CCCATATGTT CCTCTCTGCC CACTGGCTTT AGCAGATGTC 
TTGAGTGCAC CATGTCCATT CATTGTAGGA ATTGATTCAA GATATTTTGA TCTCTATGAC 
CCTCCACCAG ATGTTAGTTG TGTTGACCTA GATACCAACA CTATTTCTCA AACTGGCGAT 
AAGAAAAATG TAGCCTGGAA GATACTTCCA AAGAAACCAT GTAAAAACCT GATGAACACA 
CTGAATAATT TGCACCAGCA GTTGGCAAAA TTGCAGCAGA GACCAAGAGA TGATGGACTA 
ATGGACTTAG CAATGAATGA CTATGATTTT AATTCTGGAA AGAGGTTGCA TATGATAGAT 
TTGGAAATCC AAGAGGCATT TTTGTGTTTC ATGGCCTCTA TTTTAAAAGG TTATAGATCA 
TACTTAAGAC CAATAACACA AGCCCCTTCT GAGACAGCCA CCGATGCATC CTCTCTTTTT 
GCCCTACAAG CTTTCTTAAG AAGCCGAGAC CGGTCACATC AAAAATTCTA TAACATGATG 
ACCAAAACAC AAATGTTCAT TCGCTTCATT GAGGAATGTT CTTTTGTCAG TGATAAAGAT 
GCAAGCCTGG CATTTTTTGA TGATTGTGTA GATAAAGTGG ATATGGACAA GAGTGGTGAA 
ATGCGACTTA TAGAACTGGA TGAATCTTTC AAAAGTGAAC ATACTGTTTT TGTAACACCA 
CCTGAGATAC CCCATCTACC CAATGGTGAA GAACCCCCTT TACAGTACAG CTATAATGGA 
TTTCCAGTTC TTAGAAACAA TTTATTTGAG AGACCTGAAG GATTTCTACA AACACGAAAG 
AACAAATTGC CTTCAAAATC AAGTAGTCCT AGTAGCCCTT CTCCCATGTT CAGAAGAACA 
AAACAGGAAA TTAAATCAGC CCATAAAATT GCCAAGAGGT ACTCTTCCAT TCCTCAGATG 
TGGTCTAGGT GTCTGCTGCG CCACTGCTAT GGACTGTGGT TCATTTGTCT GCCAGCTTAT 
GTGAAAGTGT GTCATTCAAA AGTCAGGGCA CTGAAGACAG CATATGATGT GCTCAAAAAA 
ATGCAGTCGA AAAAGATGGA TCCACCTGAT GAAGTGTGCT ACCGCATTCT CATGCAGCTC 
TGTGGGCAGT ACGATCAGCC CGTGCTTGCA GTTCGAGTGC TTTTTGAAAT GCAGAAAGCT 
GGTATTGATC CCAATGCCAT TACTTATGGT TATTATAATA AGGCTGTTTT GGAAAGCACC 
TGGCCCTCAA GAAGTCGTAG TGGCTATTTT CTTTGGACAA AAGTAAGAAA CGTTGTTTTA 
GGAGTAGCAC AGTTCAAAAG AGCTTTAAAG AAACAGGCAC ACTTATCACA AACAACTCTC 
TCAGCAGATG GCAGTGACCT GGATGCTGTT AGTCATGGCA GCATGGATAG TGGTCATGGG 
ACACACACTG TGGAGCAGGC ACCTTTTAAT ACGGGTTTAA TCAAAGTATA TGCTACTGAT 
GATAGATCTA GTACAGGTGG CCAGTCTGAT CTTGGATATA ATTCATTATC TAAGGATGAA 
GTTAGAAGAG GGGATATATC TACTGAGGAA ATTCAAGAAG AAAAAGATAA GAAAGGAAGT 
GATTCTAGTT CCCTGTCAGA GAGTGAGAGT ACAAAAGGAA GTGCTGACTG TCTTCCTAAA 
CTCAATTATC AAAGTTCTTC CAGTATAGTT CGCCTCAGTG GTACAAATAA CAATGGTGCT 
GATAAAATAT CAGGAGAAAG CACAGAAAGC ACATCTAAGC TGCTCTTACT ATCATCTTTT 
GAGGGTACAA ATGAAACAGG AAATAGTCAA AATAGATGCT TCAGGAAAAG ACATAAAAGT 
GACAACGAAC CTAATTTGCA ACAGCGACTC TCCTGGGGAA GCAGAAACCG CAATCTTAGT 
GGAGGAGTGC TGATGGGACT TATGCTTAAT AGAATCAACC AGGAAGCAAA CCCTGGAGAC 
ATGGTTGAAA AACTAGGAGC TGATGCAAAA ATTCTTTCAA ACGTTATATC ACAAAGCACA 
AGACCAACTA GTCTTGATAT TGGAAAACCC CCTTTAAGAT CAAAAAGAGA CAGTCTAGAA 
AAGGAATCTA GCGACGATGA TACACCTTTC GATGGTTCTA ATTGTTTGGC AGATAAAGTG 
GATTCTCCTG TTATTTTTGA TTTGGAAGAC TTAGACAGTG AGACAGATGG ATCAAAAGTG 
GGATACATTG CTTCACAAAA TCCCAGAAGG ATTCAGCGTA TGAGCAGCAG CTTCTCGGTA 
AAACCTTCTG AAAAAACGGA TGTTGCCACA GGATTTGATC CCCTCTCTCT TTTGGTTGCT 
GAGACTGAAC AGCAGCAAAA AGAGGAAGAG GAGGAGGATG ATGAAGATAG CAAAAGCGTT 
TCAACACCAT CCGCCAGGCG TGACTTAGCC GAAGAGATTG TGATGTATAT GAATAACATG 
AGCAGTCCTT TGACAAGCCG CACACCAAGC ATCGATTTAC AACGGGCATG TGATGACAAA 
TCAACCAGTA AGAAAAGTCC AACATTTGTC AAGGCATGCA GGAGATCTAG CCTGCCTCCT 
CATTCTCCGA GGCCAGTGAG ACTTACCAAA TCTAAAAGTT ATACAAAAAC TGAGGAGAGA 
CCTCGGGACA GACTCTGGTC TTCTCCAGCC TTCTCACCTA CTTGCCCATT TAGGGAAGAA 
TCTCAAGACG CATTAACCCA TTCATCACCT TCATTTAATT TAGATACACT GCTAGTACCT 
AAACTAGATG TTCTAAGGAA CAGTATGTTC ACTGCTGGGA AAGGAGTTGC CGAGAAAGCC 
AGCAAATGGT ATTCAAGATT CACCATGTAT ACCACATCAT CTAAGGATCA AAGTTCTGAT 
CGTACCAGTC TTTCTTCAGT GGGAGCCCAG GATTCTGAAT CTACCTCTTT AACAGATGAA 
GATGTTTGCC ATGAATTGGA AGGAGCCACT TCCTCCCAAG AGAGCTGTGC TGCTTCAGGG 
AATAAGGGAA TTGATTTCAG CAGAACAAGC CTGGAAAGTT CTGCCTCCTT AGAAGGATCC 
CTGTCAAAGT TTGCCTTACC TGGGAAATCA GAAGTGGCAT CTTCCTGCAA CACAAGTAAT 
ACAAATATCT TCCAGAACTA TGCAATGGAG GTTCTTATCT CAAGTTGCTC TCGGTGTAGA 
ACTTGTGATT GTCTTGTCCA TGATGAGGAA ATCATGGCTG GCTGGACAGC AGATGATTCA 
AATCTCAATA CTACATGCCC GTTTTGTGGC AATCTCTTCT TGCCCTTTCT GAATATTGAA 
ATAAGAGATT TAAGACGACC CGGAAGATAC TTTCTGAAGT CAAGTCCATC TACAGAAAAT 
ATGCATTTTC CATCCTCCCT TTCAAGTCAG ACAAGGCAGT CTTGTATCTC AACATCAGCC 
TCTGGTCTTG ACACGTCTGT TATCTCTGTT CAAGGGAATT TTGATCTCAA TAGCAAATCT 
AAACTGCTGG AAAATACATG TGCCCGAACT ATTCACATCC CTGCTGATAG ATCAAAAACA 
GGTGTGTCAA AATGTCCAAT ATTTCCAATG GCCAGGAGTG TTAGTACTTA TGGCCCCTTG 
GATAAGGAAG ACACTGGAGG CCAGAAGCTC ATTCCTACAG GCAGCCTGCC AACTACTTTA 
CAAGGAGCTA CAGATTCTTT AGGATTAGAG TGGCACCTTC CTAGTCCAGA TCCTATCACA 
GTTCCATATC TTAGTCCTTT AGTGGTATGG AAGGAGCTTG AAAGCTTACT GGAAAATGAA 
GGCGACCATG CAATAACAGT GGCAGACTTT GTGGATCATC ATCCAATCGT GTTTTGGAAC 
CTGGTGTGGT ATTTTAGACG TCTCGACTTA CCCAGTAACT TACCTGGATT GATTCTTTCT 
TCTGAGCACT GTAACAAGTA TTCAAAGATT CCTCGCCACT GTATGTCGGA AGATAGTAAA 
TACGTTTTAA TACAAATGTT ATGGGACAAT ATGAAATTAC ATCAGGATCC GGGACAGCCT 
TTGTACATCC TCTGGAATGC CCACACCCAA AAGTATCCAA TGGTCCATTT ATTGCAAAAG 
GGTGATAATT CATTTAACCA GGAACTGTTG AAAAATATGG TAAAAAGCAT TAAAATGAAT 
GATGTCTATG GACCAATGAG TCAAATTTTA GAGACACTGA ATAAATGTCC TCATTTTAAA 
AGACAGAGGA GTTTATATAG AGAAATACTA TTTCTCTCAC TTGTGGCACT GGGAAGAGAA 
AACATTGATA TAGATGCATT TGATAAAGAA TACAAGATGG CATACGATCG TCTCACACCT 
AGTCAAGTCA AGAGTACACA CAACTGTGAT AGACCACCAA GTACAGGGGT GATGGAATGT 
CGAAAAACCT TTGGAGAACC TTATCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005638586.1
CDS	343..6072
Translation	MIEDKGPRVADYFVVAGLTDVSKPLEEEIHFNDACHKVAKPKEPITDVSVIIKSLGEEVPRDYICIDVTPTGLSADLNNGSLVGPQIYLCYRRGRDKPPLTDLGVLYDWKERLKQGCEIIQSTPYGHPANISGSTSSQKIYITYRRASENMTQNTLAVTDICIIIPSKGESPPHTFCKVDKNLNNSMWGSAVYLCYKKSVAKTNTISYKAGLICRYPQEDYESFSLPESVPLFCLPMGATIECWPPNSKYPLPVFSTFVLTGASAEKVYGAAIQFYEPYSEENLTEKQRLLLGLASSADGKSDSSKTIHTSKCICLLSHWPFFDAFRKFLTFLYRYSISGPHVLPIEKHISHFMHKVPFPSPQRPRILVQLSPHDNLILSQPVSSPLPLSGGKFSTLLQNLGPENAVTLLVFAVTEHKILIHSLRPSVLTSVTEALVSMIFPFHWPCPYVPLCPLALADVLSAPCPFIVGIDSRYFDLYDPPPDVSCVDLDTNTISQTGDKKNVAWKILPKKPCKNLMNTLNNLHQQLAKLQQRPRDDGLMDLAMNDYDFNSGKRLHMIDLEIQEAFLCFMASILKGYRSYLRPITQAPSETATDASSLFALQAFLRSRDRSHQKFYNMMTKTQMFIRFIEECSFVSDKDASLAFFDDCVDKVDMDKSGEMRLIELDESFKSEHTVFVTPPEIPHLPNGEEPPLQYSYNGFPVLRNNLFERPEGFLQTRKNKLPSKSSSPSSPSPMFRRTKQEIKSAHKIAKRYSSIPQMWSRCLLRHCYGLWFICLPAYVKVCHSKVRALKTAYDVLKKMQSKKMDPPDEVCYRILMQLCGQYDQPVLAVRVLFEMQKAGIDPNAITYGYYNKAVLESTWPSRSRSGYFLWTKVRNVVLGVAQFKRALKKQAHLSQTTLSADGSDLDAVSHGSMDSGHGTHTVEQAPFNTGLIKVYATDDRSSTGGQSDLGYNSLSKDEVRRGDISTEEIQEEKDKKGSDSSSLSESESTKGSADCLPKLNYQSSSSIVRLSGTNNNGADKISGESTESTSKLLLLSSFEGTNETGNSQNRCFRKRHKSDNEPNLQQRLSWGSRNRNLSGGVLMGLMLNRINQEANPGDMVEKLGADAKILSNVISQSTRPTSLDIGKPPLRSKRDSLEKESSDDDTPFDGSNCLADKVDSPVIFDLEDLDSETDGSKVGYIASQNPRRIQRMSSSFSVKPSEKTDVATGFDPLSLLVAETEQQQKEEEEEDDEDSKSVSTPSARRDLAEEIVMYMNNMSSPLTSRTPSIDLQRACDDKSTSKKSPTFVKACRRSSLPPHSPRPVRLTKSKSYTKTEERPRDRLWSSPAFSPTCPFREESQDALTHSSPSFNLDTLLVPKLDVLRNSMFTAGKGVAEKASKWYSRFTMYTTSSKDQSSDRTSLSSVGAQDSESTSLTDEDVCHELEGATSSQESCAASGNKGIDFSRTSLESSASLEGSLSKFALPGKSEVASSCNTSNTNIFQNYAMEVLISSCSRCRTCDCLVHDEEIMAGWTADDSNLNTTCPFCGNLFLPFLNIEIRDLRRPGRYFLKSSPSTENMHFPSSLSSQTRQSCISTSASGLDTSVISVQGNFDLNSKSKLLENTCARTIHIPADRSKTGVSKCPIFPMARSVSTYGPLDKEDTGGQKLIPTGSLPTTLQGATDSLGLEWHLPSPDPITVPYLSPLVVWKELESLLENEGDHAITVADFVDHHPIVFWNLVWYFRRLDLPSNLPGLILSSEHCNKYSKIPRHCMSEDSKYVLIQMLWDNMKLHQDPGQPLYILWNAHTQKYPMVHLLQKGDNSFNQELLKNMVKSIKMNDVYGPMSQILETLNKCPHFKRQRSLYREILFLSLVALGRENIDIDAFDKEYKMAYDRLTPSQVKSTHNCDRPPSTGVMECRKTFGEPYL

Target ORF information:

RefSeq Version	XM_005638529.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris DENN domain containing 4A (DENND4A), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005638529.3

ORF Insert Sequence:

ATGATTGAAG ATAAGGGTCC TCGTGTTGCT GACTACTTTG TTGTAGCAGG ATTAACTGAT 
GTTTCAAAGC CTCTTGAAGA AGAAATTCAC TTCAATGATG CCTGTCATAA AGTAGCTAAA 
CCAAAAGAAC CTATCACAGA TGTTTCGGTG ATTATTAAAT CTCTTGGGGA AGAAGTGCCA 
CGGGATTATA TATGTATTGA TGTTACCCCA ACTGGATTGT CGGCTGATCT CAATAATGGA 
AGTCTTGTGG GGCCACAGAT TTACCTTTGC TACAGAAGAG GAAGAGATAA GCCTCCACTT 
ACAGATCTGG GGGTTTTGTA TGACTGGAAA GAAAGATTGA AACAGGGATG TGAAATTATT 
CAGAGTACTC CCTATGGACA CCCTGCAAAT ATTAGTGGCA GTACCTCATC ACAAAAAATT 
TATATAACTT ACCGAAGAGC CTCTGAAAAC ATGACGCAAA ATACGTTGGC TGTCACAGAC 
ATATGTATTA TTATACCCAG TAAAGGAGAG AGTCCACCAC ACACGTTTTG CAAAGTCGAT 
AAGAATCTCA ATAACAGTAT GTGGGGATCT GCAGTGTACT TATGTTATAA AAAATCAGTG 
GCAAAGACTA ACACTATATC CTATAAAGCT GGTCTAATTT GTAGATATCC TCAAGAAGAT 
TACGAATCTT TCTCACTCCC AGAATCTGTT CCCCTATTTT GTTTACCAAT GGGTGCAACT 
ATTGAATGTT GGCCACCAAA TAGCAAATAC CCTCTCCCTG TATTTTCTAC ATTTGTGTTA 
ACTGGAGCCT CAGCTGAAAA GGTCTATGGT GCTGCTATTC AGTTTTATGA ACCATACTCT 
GAGGAGAATC TCACAGAAAA ACAGAGACTT CTTTTGGGTT TAGCATCATC AGCAGATGGG 
AAATCTGATA GTTCTAAAAC AATTCACACT AGTAAATGCA TCTGTCTTCT TTCTCACTGG 
CCTTTTTTTG ATGCATTCAG GAAATTTTTG ACCTTTCTGT ATCGTTACTC TATCTCTGGA 
CCTCATGTCC TTCCAATTGA GAAGCATATT TCTCATTTTA TGCATAAAGT TCCTTTTCCA 
TCTCCTCAGA GGCCACGGAT TTTAGTTCAG TTATCGCCAC ATGATAATCT GATTCTCAGT 
CAACCTGTTT CTTCACCTCT TCCACTAAGT GGTGGCAAAT TTTCAACACT ACTTCAAAAT 
TTAGGCCCTG AAAATGCTGT AACACTACTG GTGTTTGCAG TAACAGAGCA CAAGATTCTC 
ATCCATTCCT TACGGCCTTC CGTGCTTACT AGCGTGACAG AAGCATTAGT GTCTATGATT 
TTCCCTTTCC ACTGGCCGTG CCCATATGTT CCTCTCTGCC CACTGGCTTT AGCAGATGTC 
TTGAGTGCAC CATGTCCATT CATTGTAGGA ATTGATTCAA GATATTTTGA TCTCTATGAC 
CCTCCACCAG ATGTTAGTTG TGTTGACCTA GATACCAACA CTATTTCTCA AACTGGCGAT 
AAGAAAAATG TAGCCTGGAA GATACTTCCA AAGAAACCAT GTAAAAACCT GATGAACACA 
CTGAATAATT TGCACCAGCA GTTGGCAAAA TTGCAGCAGA GACCAAGAGA TGATGGACTA 
ATGGACTTAG CAATGAATGA CTATGATTTT AATTCTGGAA AGAGGTTGCA TATGATAGAT 
TTGGAAATCC AAGAGGCATT TTTGTGTTTC ATGGCCTCTA TTTTAAAAGG TTATAGATCA 
TACTTAAGAC CAATAACACA AGCCCCTTCT GAGACAGCCA CCGATGCATC CTCTCTTTTT 
GCCCTACAAG CTTTCTTAAG AAGCCGAGAC CGGTCACATC AAAAATTCTA TAACATGATG 
ACCAAAACAC AAATGTTCAT TCGCTTCATT GAGGAATGTT CTTTTGTCAG TGATAAAGAT 
GCAAGCCTGG CATTTTTTGA TGATTGTGTA GATAAAGTGG ATATGGACAA GAGTGGTGAA 
ATGCGACTTA TAGAACTGGA TGAATCTTTC AAAAGTGAAC ATACTGTTTT TGTAACACCA 
CCTGAGATAC CCCATCTACC CAATGGTGAA GAACCCCCTT TACAGTACAG CTATAATGGA 
TTTCCAGTTC TTAGAAACAA TTTATTTGAG AGACCTGAAG GATTTCTACA AACACGAAAG 
AACAAATTGC CTTCAAAATC AAGTAGTCCT AGTAGCCCTT CTCCCATGTT CAGAAGAACA 
AAACAGGAAA TTAAATCAGC CCATAAAATT GCCAAGAGGT ACTCTTCCAT TCCTCAGATG 
TGGTCTAGGT GTCTGCTGCG CCACTGCTAT GGACTGTGGT TCATTTGTCT GCCAGCTTAT 
GTGAAAGTGT GTCATTCAAA AGTCAGGGCA CTGAAGACAG CATATGATGT GCTCAAAAAA 
ATGCAGTCGA AAAAGATGGA TCCACCTGAT GAAGTGTGCT ACCGCATTCT CATGCAGCTC 
TGTGGGCAGT ACGATCAGCC CGTGCTTGCA GTTCGAGTGC TTTTTGAAAT GCAGAAAGCT 
GGTATTGATC CCAATGCCAT TACTTATGGT TATTATAATA AGGCTGTTTT GGAAAGCACC 
TGGCCCTCAA GAAGTCGTAG TGGCTATTTT CTTTGGACAA AAGTAAGAAA CGTTGTTTTA 
GGAGTAGCAC AGTTCAAAAG AGCTTTAAAG AAACAGGCAC ACTTATCACA AACAACTCTC 
TCAGCAGATG GCAGTGACCT GGATGCTGTT AGTCATGGCA GCATGGATAG TGGTCATGGG 
ACACACACTG TGGAGCAGGC ACCTTTTAAT ACGGGTTTAA TCAAAGTATA TGCTACTGAT 
GATAGATCTA GTACAGGTGG CCAGTCTGAT CTTGGATATA ATTCATTATC TAAGGATGAA 
GTTAGAAGAG GGGATATATC TACTGAGGAA ATTCAAGAAG AAAAAGATAA GAAAGGAAGT 
GATTCTAGTT CCCTGTCAGA GAGTGAGAGT ACAAAAGGAA GTGCTGACTG TCTTCCTAAA 
CTCAATTATC AAAGTTCTTC CAGTATAGTT CGCCTCAGTG GTACAAATAA CAATGGTGCT 
GATAAAATAT CAGGAGAAAG CACAGAAAGC ACATCTAAGC TGCTCTTACT ATCATCTTTT 
GAGGGTACAA ATGAAACAGG AAATAGTCAA AATAGATGCT TCAGGAAAAG ACATAAAAGT 
GACAACGAAC CTAATTTGCA ACAGCGACTC TCCTGGGGAA GCAGAAACCG CAATCTTAGT 
GGAGGAGTGC TGATGGGACT TATGCTTAAT AGAATCAACC AGGAAGCAAA CCCTGGAGAC 
ATGGTTGAAA AACTAGGAGC TGATGCAAAA ATTCTTTCAA ACGTTATATC ACAAAGCACA 
AGACCAACTA GTCTTGATAT TGGAAAACCC CCTTTAAGAT CAAAAAGAGA CAGTCTAGAA 
AAGGAATCTA GCGACGATGA TACACCTTTC GATGGTTCTA ATTGTTTGGC AGATAAAGTG 
GATTCTCCTG TTATTTTTGA TTTGGAAGAC TTAGACAGTG AGACAGATGG ATCAAAAGTG 
GGATACATTG CTTCACAAAA TCCCAGAAGG ATTCAGCGTA TGAGCAGCAG CTTCTCGGTA 
AAACCTTCTG AAAAAACGGA TGTTGCCACA GGATTTGATC CCCTCTCTCT TTTGGTTGCT 
GAGACTGAAC AGCAGCAAAA AGAGGAAGAG GAGGAGGATG ATGAAGATAG CAAAAGCGTT 
TCAACACCAT CCGCCAGGCG TGACTTAGCC GAAGAGATTG TGATGTATAT GAATAACATG 
AGCAGTCCTT TGACAAGCCG CACACCAAGC ATCGATTTAC AACGGGCATG TGATGACAAA 
TCAACCAGTA AGAAAAGTCC AACATTTGTC AAGGCATGCA GGAGATCTAG CCTGCCTCCT 
CATTCTCCGA GGCCAGTGAG ACTTACCAAA TCTAAAAGTT ATACAAAAAC TGAGGAGAGA 
CCTCGGGACA GACTCTGGTC TTCTCCAGCC TTCTCACCTA CTTGCCCATT TAGGGAAGAA 
TCTCAAGACG CATTAACCCA TTCATCACCT TCATTTAATT TAGATACACT GCTAGTACCT 
AAACTAGATG TTCTAAGGAA CAGTATGTTC ACTGCTGGGA AAGGAGTTGC CGAGAAAGCC 
AGCAAATGGT ATTCAAGATT CACCATGTAT ACCACATCAT CTAAGGATCA AAGTTCTGAT 
CGTACCAGTC TTTCTTCAGT GGGAGCCCAG GATTCTGAAT CTACCTCTTT AACAGATGAA 
GATGTTTGCC ATGAATTGGA AGGAGCCACT TCCTCCCAAG AGAGCTGTGC TGCTTCAGGG 
AATAAGGGAA TTGATTTCAG CAGAACAAGC CTGGAAAGTT CTGCCTCCTT AGAAGGATCC 
CTGTCAAAGT TTGCCTTACC TGGGAAATCA GAAGTGGCAT CTTCCTGCAA CACAAGTAAT 
ACAAATATCT TCCAGAACTA TGCAATGGAG GTTCTTATCT CAAGTTGCTC TCGGTGTAGA 
ACTTGTGATT GTCTTGTCCA TGATGAGGAA ATCATGGCTG GCTGGACAGC AGATGATTCA 
AATCTCAATA CTACATGCCC GTTTTGTGGC AATCTCTTCT TGCCCTTTCT GAATATTGAA 
ATAAGAGATT TAAGACGACC CGGAAGATAC TTTCTGAAGT CAAGTCCATC TACAGAAAAT 
ATGCATTTTC CATCCTCCCT TTCAAGTCAG ACAAGGCAGT CTTGTATCTC AACATCAGCC 
TCTGGTCTTG ACACGTCTGT TATCTCTGTT CAAGGGAATT TTGATCTCAA TAGCAAATCT 
AAACTGCTGG AAAATACATG TGCCCGAACT ATTCACATCC CTGCTGATAG ATCAAAAACA 
GGTGTGTCAA AATGTCCAAT ATTTCCAATG GCCAGGAGTG TTAGTACTTA TGGCCCCTTG 
GATAAGGAAG ACACTGGAGG CCAGAAGCTC ATTCCTACAG GCAGCCTGCC AACTACTTTA 
CAAGGAGCTA CAGATTCTTT AGGATTAGAG TGGCACCTTC CTAGTCCAGA TCCTATCACA 
GTTCCATATC TTAGTCCTTT AGTGGTATGG AAGGAGCTTG AAAGCTTACT GGAAAATGAA 
GGCGACCATG CAATAACAGT GGCAGACTTT GTGGATCATC ATCCAATCGT GTTTTGGAAC 
CTGGTGTGGT ATTTTAGACG TCTCGACTTA CCCAGTAACT TACCTGGATT GATTCTTTCT 
TCTGAGCACT GTAACAAGTA TTCAAAGATT CCTCGCCACT GTATGTCGGA AGATAGTAAA 
TACGTTTTAA TACAAATGTT ATGGGACAAT ATGAAATTAC ATCAGGATCC GGGACAGCCT 
TTGTACATCC TCTGGAATGC CCACACCCAA AAGTATCCAA TGGTCCATTT ATTGCAAAAG 
GGTGATAATT CATTTAACCA GGAACTGTTG AAAAATATGG TAAAAAGCAT TAAAATGAAT 
GATGTCTATG GACCAATGAG TCAAATTTTA GAGACACTGA ATAAATGTCC TCATTTTAAA 
AGACAGAGGA GTTTATATAG AGAAATACTA TTTCTCTCAC TTGTGGCACT GGGAAGAGAA 
AACATTGATA TAGATGCATT TGATAAAGAA TACAAGATGG CATACGATCG TCTCACACCT 
AGTCAAGTCA AGAGTACACA CAACTGTGAT AGACCACCAA GTACAGGGGT GATGGAATGT 
CGAAAAACCT TTGGAGAACC TTATCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DENND4A ( XM_005638529.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005638586.1 Canis lupus familiaris DENN/MADD domain containing 4A (DENND4A), transcript variant X3, mRNA.

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CloneID	OCe29020
Clone ID Related Accession (Same CDS sequence)	XM_005638529.3 , XM_005638529.2
Accession Version	XM_005638529.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5730bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	C-myc promoter-binding protein isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726113.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005638529.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTGAAG ATAAGGGTCC TCGTGTTGCT GACTACTTTG TTGTAGCAGG ATTAACTGAT 
GTTTCAAAGC CTCTTGAAGA AGAAATTCAC TTCAATGATG CCTGTCATAA AGTAGCTAAA 
CCAAAAGAAC CTATCACAGA TGTTTCGGTG ATTATTAAAT CTCTTGGGGA AGAAGTGCCA 
CGGGATTATA TATGTATTGA TGTTACCCCA ACTGGATTGT CGGCTGATCT CAATAATGGA 
AGTCTTGTGG GGCCACAGAT TTACCTTTGC TACAGAAGAG GAAGAGATAA GCCTCCACTT 
ACAGATCTGG GGGTTTTGTA TGACTGGAAA GAAAGATTGA AACAGGGATG TGAAATTATT 
CAGAGTACTC CCTATGGACA CCCTGCAAAT ATTAGTGGCA GTACCTCATC ACAAAAAATT 
TATATAACTT ACCGAAGAGC CTCTGAAAAC ATGACGCAAA ATACGTTGGC TGTCACAGAC 
ATATGTATTA TTATACCCAG TAAAGGAGAG AGTCCACCAC ACACGTTTTG CAAAGTCGAT 
AAGAATCTCA ATAACAGTAT GTGGGGATCT GCAGTGTACT TATGTTATAA AAAATCAGTG 
GCAAAGACTA ACACTATATC CTATAAAGCT GGTCTAATTT GTAGATATCC TCAAGAAGAT 
TACGAATCTT TCTCACTCCC AGAATCTGTT CCCCTATTTT GTTTACCAAT GGGTGCAACT 
ATTGAATGTT GGCCACCAAA TAGCAAATAC CCTCTCCCTG TATTTTCTAC ATTTGTGTTA 
ACTGGAGCCT CAGCTGAAAA GGTCTATGGT GCTGCTATTC AGTTTTATGA ACCATACTCT 
GAGGAGAATC TCACAGAAAA ACAGAGACTT CTTTTGGGTT TAGCATCATC AGCAGATGGG 
AAATCTGATA GTTCTAAAAC AATTCACACT AGTAAATGCA TCTGTCTTCT TTCTCACTGG 
CCTTTTTTTG ATGCATTCAG GAAATTTTTG ACCTTTCTGT ATCGTTACTC TATCTCTGGA 
CCTCATGTCC TTCCAATTGA GAAGCATATT TCTCATTTTA TGCATAAAGT TCCTTTTCCA 
TCTCCTCAGA GGCCACGGAT TTTAGTTCAG TTATCGCCAC ATGATAATCT GATTCTCAGT 
CAACCTGTTT CTTCACCTCT TCCACTAAGT GGTGGCAAAT TTTCAACACT ACTTCAAAAT 
TTAGGCCCTG AAAATGCTGT AACACTACTG GTGTTTGCAG TAACAGAGCA CAAGATTCTC 
ATCCATTCCT TACGGCCTTC CGTGCTTACT AGCGTGACAG AAGCATTAGT GTCTATGATT 
TTCCCTTTCC ACTGGCCGTG CCCATATGTT CCTCTCTGCC CACTGGCTTT AGCAGATGTC 
TTGAGTGCAC CATGTCCATT CATTGTAGGA ATTGATTCAA GATATTTTGA TCTCTATGAC 
CCTCCACCAG ATGTTAGTTG TGTTGACCTA GATACCAACA CTATTTCTCA AACTGGCGAT 
AAGAAAAATG TAGCCTGGAA GATACTTCCA AAGAAACCAT GTAAAAACCT GATGAACACA 
CTGAATAATT TGCACCAGCA GTTGGCAAAA TTGCAGCAGA GACCAAGAGA TGATGGACTA 
ATGGACTTAG CAATGAATGA CTATGATTTT AATTCTGGAA AGAGGTTGCA TATGATAGAT 
TTGGAAATCC AAGAGGCATT TTTGTGTTTC ATGGCCTCTA TTTTAAAAGG TTATAGATCA 
TACTTAAGAC CAATAACACA AGCCCCTTCT GAGACAGCCA CCGATGCATC CTCTCTTTTT 
GCCCTACAAG CTTTCTTAAG AAGCCGAGAC CGGTCACATC AAAAATTCTA TAACATGATG 
ACCAAAACAC AAATGTTCAT TCGCTTCATT GAGGAATGTT CTTTTGTCAG TGATAAAGAT 
GCAAGCCTGG CATTTTTTGA TGATTGTGTA GATAAAGTGG ATATGGACAA GAGTGGTGAA 
ATGCGACTTA TAGAACTGGA TGAATCTTTC AAAAGTGAAC ATACTGTTTT TGTAACACCA 
CCTGAGATAC CCCATCTACC CAATGGTGAA GAACCCCCTT TACAGTACAG CTATAATGGA 
TTTCCAGTTC TTAGAAACAA TTTATTTGAG AGACCTGAAG GATTTCTACA AACACGAAAG 
AACAAATTGC CTTCAAAATC AAGTAGTCCT AGTAGCCCTT CTCCCATGTT CAGAAGAACA 
AAACAGGAAA TTAAATCAGC CCATAAAATT GCCAAGAGGT ACTCTTCCAT TCCTCAGATG 
TGGTCTAGGT GTCTGCTGCG CCACTGCTAT GGACTGTGGT TCATTTGTCT GCCAGCTTAT 
GTGAAAGTGT GTCATTCAAA AGTCAGGGCA CTGAAGACAG CATATGATGT GCTCAAAAAA 
ATGCAGTCGA AAAAGATGGA TCCACCTGAT GAAGTGTGCT ACCGCATTCT CATGCAGCTC 
TGTGGGCAGT ACGATCAGCC CGTGCTTGCA GTTCGAGTGC TTTTTGAAAT GCAGAAAGCT 
GGTATTGATC CCAATGCCAT TACTTATGGT TATTATAATA AGGCTGTTTT GGAAAGCACC 
TGGCCCTCAA GAAGTCGTAG TGGCTATTTT CTTTGGACAA AAGTAAGAAA CGTTGTTTTA 
GGAGTAGCAC AGTTCAAAAG AGCTTTAAAG AAACAGGCAC ACTTATCACA AACAACTCTC 
TCAGCAGATG GCAGTGACCT GGATGCTGTT AGTCATGGCA GCATGGATAG TGGTCATGGG 
ACACACACTG TGGAGCAGGC ACCTTTTAAT ACGGGTTTAA TCAAAGTATA TGCTACTGAT 
GATAGATCTA GTACAGGTGG CCAGTCTGAT CTTGGATATA ATTCATTATC TAAGGATGAA 
GTTAGAAGAG GGGATATATC TACTGAGGAA ATTCAAGAAG AAAAAGATAA GAAAGGAAGT 
GATTCTAGTT CCCTGTCAGA GAGTGAGAGT ACAAAAGGAA GTGCTGACTG TCTTCCTAAA 
CTCAATTATC AAAGTTCTTC CAGTATAGTT CGCCTCAGTG GTACAAATAA CAATGGTGCT 
GATAAAATAT CAGGAGAAAG CACAGAAAGC ACATCTAAGC TGCTCTTACT ATCATCTTTT 
GAGGGTACAA ATGAAACAGG AAATAGTCAA AATAGATGCT TCAGGAAAAG ACATAAAAGT 
GACAACGAAC CTAATTTGCA ACAGCGACTC TCCTGGGGAA GCAGAAACCG CAATCTTAGT 
GGAGGAGTGC TGATGGGACT TATGCTTAAT AGAATCAACC AGGAAGCAAA CCCTGGAGAC 
ATGGTTGAAA AACTAGGAGC TGATGCAAAA ATTCTTTCAA ACGTTATATC ACAAAGCACA 
AGACCAACTA GTCTTGATAT TGGAAAACCC CCTTTAAGAT CAAAAAGAGA CAGTCTAGAA 
AAGGAATCTA GCGACGATGA TACACCTTTC GATGGTTCTA ATTGTTTGGC AGATAAAGTG 
GATTCTCCTG TTATTTTTGA TTTGGAAGAC TTAGACAGTG AGACAGATGG ATCAAAAGTG 
GGATACATTG CTTCACAAAA TCCCAGAAGG ATTCAGCGTA TGAGCAGCAG CTTCTCGGTA 
AAACCTTCTG AAAAAACGGA TGTTGCCACA GGATTTGATC CCCTCTCTCT TTTGGTTGCT 
GAGACTGAAC AGCAGCAAAA AGAGGAAGAG GAGGAGGATG ATGAAGATAG CAAAAGCGTT 
TCAACACCAT CCGCCAGGCG TGACTTAGCC GAAGAGATTG TGATGTATAT GAATAACATG 
AGCAGTCCTT TGACAAGCCG CACACCAAGC ATCGATTTAC AACGGGCATG TGATGACAAA 
TCAACCAGTA AGAAAAGTCC AACATTTGTC AAGGCATGCA GGAGATCTAG CCTGCCTCCT 
CATTCTCCGA GGCCAGTGAG ACTTACCAAA TCTAAAAGTT ATACAAAAAC TGAGGAGAGA 
CCTCGGGACA GACTCTGGTC TTCTCCAGCC TTCTCACCTA CTTGCCCATT TAGGGAAGAA 
TCTCAAGACG CATTAACCCA TTCATCACCT TCATTTAATT TAGATACACT GCTAGTACCT 
AAACTAGATG TTCTAAGGAA CAGTATGTTC ACTGCTGGGA AAGGAGTTGC CGAGAAAGCC 
AGCAAATGGT ATTCAAGATT CACCATGTAT ACCACATCAT CTAAGGATCA AAGTTCTGAT 
CGTACCAGTC TTTCTTCAGT GGGAGCCCAG GATTCTGAAT CTACCTCTTT AACAGATGAA 
GATGTTTGCC ATGAATTGGA AGGAGCCACT TCCTCCCAAG AGAGCTGTGC TGCTTCAGGG 
AATAAGGGAA TTGATTTCAG CAGAACAAGC CTGGAAAGTT CTGCCTCCTT AGAAGGATCC 
CTGTCAAAGT TTGCCTTACC TGGGAAATCA GAAGTGGCAT CTTCCTGCAA CACAAGTAAT 
ACAAATATCT TCCAGAACTA TGCAATGGAG GTTCTTATCT CAAGTTGCTC TCGGTGTAGA 
ACTTGTGATT GTCTTGTCCA TGATGAGGAA ATCATGGCTG GCTGGACAGC AGATGATTCA 
AATCTCAATA CTACATGCCC GTTTTGTGGC AATCTCTTCT TGCCCTTTCT GAATATTGAA 
ATAAGAGATT TAAGACGACC CGGAAGATAC TTTCTGAAGT CAAGTCCATC TACAGAAAAT 
ATGCATTTTC CATCCTCCCT TTCAAGTCAG ACAAGGCAGT CTTGTATCTC AACATCAGCC 
TCTGGTCTTG ACACGTCTGT TATCTCTGTT CAAGGGAATT TTGATCTCAA TAGCAAATCT 
AAACTGCTGG AAAATACATG TGCCCGAACT ATTCACATCC CTGCTGATAG ATCAAAAACA 
GGTGTGTCAA AATGTCCAAT ATTTCCAATG GCCAGGAGTG TTAGTACTTA TGGCCCCTTG 
GATAAGGAAG ACACTGGAGG CCAGAAGCTC ATTCCTACAG GCAGCCTGCC AACTACTTTA 
CAAGGAGCTA CAGATTCTTT AGGATTAGAG TGGCACCTTC CTAGTCCAGA TCCTATCACA 
GTTCCATATC TTAGTCCTTT AGTGGTATGG AAGGAGCTTG AAAGCTTACT GGAAAATGAA 
GGCGACCATG CAATAACAGT GGCAGACTTT GTGGATCATC ATCCAATCGT GTTTTGGAAC 
CTGGTGTGGT ATTTTAGACG TCTCGACTTA CCCAGTAACT TACCTGGATT GATTCTTTCT 
TCTGAGCACT GTAACAAGTA TTCAAAGATT CCTCGCCACT GTATGTCGGA AGATAGTAAA 
TACGTTTTAA TACAAATGTT ATGGGACAAT ATGAAATTAC ATCAGGATCC GGGACAGCCT 
TTGTACATCC TCTGGAATGC CCACACCCAA AAGTATCCAA TGGTCCATTT ATTGCAAAAG 
GGTGATAATT CATTTAACCA GGAACTGTTG AAAAATATGG TAAAAAGCAT TAAAATGAAT 
GATGTCTATG GACCAATGAG TCAAATTTTA GAGACACTGA ATAAATGTCC TCATTTTAAA 
AGACAGAGGA GTTTATATAG AGAAATACTA TTTCTCTCAC TTGTGGCACT GGGAAGAGAA 
AACATTGATA TAGATGCATT TGATAAAGAA TACAAGATGG CATACGATCG TCTCACACCT 
AGTCAAGTCA AGAGTACACA CAACTGTGAT AGACCACCAA GTACAGGGGT GATGGAATGT 
CGAAAAACCT TTGGAGAACC TTATCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005638586.1
CDS	378..6107
Translation	MIEDKGPRVADYFVVAGLTDVSKPLEEEIHFNDACHKVAKPKEPITDVSVIIKSLGEEVPRDYICIDVTPTGLSADLNNGSLVGPQIYLCYRRGRDKPPLTDLGVLYDWKERLKQGCEIIQSTPYGHPANISGSTSSQKIYITYRRASENMTQNTLAVTDICIIIPSKGESPPHTFCKVDKNLNNSMWGSAVYLCYKKSVAKTNTISYKAGLICRYPQEDYESFSLPESVPLFCLPMGATIECWPPNSKYPLPVFSTFVLTGASAEKVYGAAIQFYEPYSEENLTEKQRLLLGLASSADGKSDSSKTIHTSKCICLLSHWPFFDAFRKFLTFLYRYSISGPHVLPIEKHISHFMHKVPFPSPQRPRILVQLSPHDNLILSQPVSSPLPLSGGKFSTLLQNLGPENAVTLLVFAVTEHKILIHSLRPSVLTSVTEALVSMIFPFHWPCPYVPLCPLALADVLSAPCPFIVGIDSRYFDLYDPPPDVSCVDLDTNTISQTGDKKNVAWKILPKKPCKNLMNTLNNLHQQLAKLQQRPRDDGLMDLAMNDYDFNSGKRLHMIDLEIQEAFLCFMASILKGYRSYLRPITQAPSETATDASSLFALQAFLRSRDRSHQKFYNMMTKTQMFIRFIEECSFVSDKDASLAFFDDCVDKVDMDKSGEMRLIELDESFKSEHTVFVTPPEIPHLPNGEEPPLQYSYNGFPVLRNNLFERPEGFLQTRKNKLPSKSSSPSSPSPMFRRTKQEIKSAHKIAKRYSSIPQMWSRCLLRHCYGLWFICLPAYVKVCHSKVRALKTAYDVLKKMQSKKMDPPDEVCYRILMQLCGQYDQPVLAVRVLFEMQKAGIDPNAITYGYYNKAVLESTWPSRSRSGYFLWTKVRNVVLGVAQFKRALKKQAHLSQTTLSADGSDLDAVSHGSMDSGHGTHTVEQAPFNTGLIKVYATDDRSSTGGQSDLGYNSLSKDEVRRGDISTEEIQEEKDKKGSDSSSLSESESTKGSADCLPKLNYQSSSSIVRLSGTNNNGADKISGESTESTSKLLLLSSFEGTNETGNSQNRCFRKRHKSDNEPNLQQRLSWGSRNRNLSGGVLMGLMLNRINQEANPGDMVEKLGADAKILSNVISQSTRPTSLDIGKPPLRSKRDSLEKESSDDDTPFDGSNCLADKVDSPVIFDLEDLDSETDGSKVGYIASQNPRRIQRMSSSFSVKPSEKTDVATGFDPLSLLVAETEQQQKEEEEEDDEDSKSVSTPSARRDLAEEIVMYMNNMSSPLTSRTPSIDLQRACDDKSTSKKSPTFVKACRRSSLPPHSPRPVRLTKSKSYTKTEERPRDRLWSSPAFSPTCPFREESQDALTHSSPSFNLDTLLVPKLDVLRNSMFTAGKGVAEKASKWYSRFTMYTTSSKDQSSDRTSLSSVGAQDSESTSLTDEDVCHELEGATSSQESCAASGNKGIDFSRTSLESSASLEGSLSKFALPGKSEVASSCNTSNTNIFQNYAMEVLISSCSRCRTCDCLVHDEEIMAGWTADDSNLNTTCPFCGNLFLPFLNIEIRDLRRPGRYFLKSSPSTENMHFPSSLSSQTRQSCISTSASGLDTSVISVQGNFDLNSKSKLLENTCARTIHIPADRSKTGVSKCPIFPMARSVSTYGPLDKEDTGGQKLIPTGSLPTTLQGATDSLGLEWHLPSPDPITVPYLSPLVVWKELESLLENEGDHAITVADFVDHHPIVFWNLVWYFRRLDLPSNLPGLILSSEHCNKYSKIPRHCMSEDSKYVLIQMLWDNMKLHQDPGQPLYILWNAHTQKYPMVHLLQKGDNSFNQELLKNMVKSIKMNDVYGPMSQILETLNKCPHFKRQRSLYREILFLSLVALGRENIDIDAFDKEYKMAYDRLTPSQVKSTHNCDRPPSTGVMECRKTFGEPYL

Target ORF information:

RefSeq Version	XM_005638529.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris DENN/MADD domain containing 4A (DENND4A), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005638529.2

ORF Insert Sequence:

ATGATTGAAG ATAAGGGTCC TCGTGTTGCT GACTACTTTG TTGTAGCAGG ATTAACTGAT 
GTTTCAAAGC CTCTTGAAGA AGAAATTCAC TTCAATGATG CCTGTCATAA AGTAGCTAAA 
CCAAAAGAAC CTATCACAGA TGTTTCGGTG ATTATTAAAT CTCTTGGGGA AGAAGTGCCA 
CGGGATTATA TATGTATTGA TGTTACCCCA ACTGGATTGT CGGCTGATCT CAATAATGGA 
AGTCTTGTGG GGCCACAGAT TTACCTTTGC TACAGAAGAG GAAGAGATAA GCCTCCACTT 
ACAGATCTGG GGGTTTTGTA TGACTGGAAA GAAAGATTGA AACAGGGATG TGAAATTATT 
CAGAGTACTC CCTATGGACA CCCTGCAAAT ATTAGTGGCA GTACCTCATC ACAAAAAATT 
TATATAACTT ACCGAAGAGC CTCTGAAAAC ATGACGCAAA ATACGTTGGC TGTCACAGAC 
ATATGTATTA TTATACCCAG TAAAGGAGAG AGTCCACCAC ACACGTTTTG CAAAGTCGAT 
AAGAATCTCA ATAACAGTAT GTGGGGATCT GCAGTGTACT TATGTTATAA AAAATCAGTG 
GCAAAGACTA ACACTATATC CTATAAAGCT GGTCTAATTT GTAGATATCC TCAAGAAGAT 
TACGAATCTT TCTCACTCCC AGAATCTGTT CCCCTATTTT GTTTACCAAT GGGTGCAACT 
ATTGAATGTT GGCCACCAAA TAGCAAATAC CCTCTCCCTG TATTTTCTAC ATTTGTGTTA 
ACTGGAGCCT CAGCTGAAAA GGTCTATGGT GCTGCTATTC AGTTTTATGA ACCATACTCT 
GAGGAGAATC TCACAGAAAA ACAGAGACTT CTTTTGGGTT TAGCATCATC AGCAGATGGG 
AAATCTGATA GTTCTAAAAC AATTCACACT AGTAAATGCA TCTGTCTTCT TTCTCACTGG 
CCTTTTTTTG ATGCATTCAG GAAATTTTTG ACCTTTCTGT ATCGTTACTC TATCTCTGGA 
CCTCATGTCC TTCCAATTGA GAAGCATATT TCTCATTTTA TGCATAAAGT TCCTTTTCCA 
TCTCCTCAGA GGCCACGGAT TTTAGTTCAG TTATCGCCAC ATGATAATCT GATTCTCAGT 
CAACCTGTTT CTTCACCTCT TCCACTAAGT GGTGGCAAAT TTTCAACACT ACTTCAAAAT 
TTAGGCCCTG AAAATGCTGT AACACTACTG GTGTTTGCAG TAACAGAGCA CAAGATTCTC 
ATCCATTCCT TACGGCCTTC CGTGCTTACT AGCGTGACAG AAGCATTAGT GTCTATGATT 
TTCCCTTTCC ACTGGCCGTG CCCATATGTT CCTCTCTGCC CACTGGCTTT AGCAGATGTC 
TTGAGTGCAC CATGTCCATT CATTGTAGGA ATTGATTCAA GATATTTTGA TCTCTATGAC 
CCTCCACCAG ATGTTAGTTG TGTTGACCTA GATACCAACA CTATTTCTCA AACTGGCGAT 
AAGAAAAATG TAGCCTGGAA GATACTTCCA AAGAAACCAT GTAAAAACCT GATGAACACA 
CTGAATAATT TGCACCAGCA GTTGGCAAAA TTGCAGCAGA GACCAAGAGA TGATGGACTA 
ATGGACTTAG CAATGAATGA CTATGATTTT AATTCTGGAA AGAGGTTGCA TATGATAGAT 
TTGGAAATCC AAGAGGCATT TTTGTGTTTC ATGGCCTCTA TTTTAAAAGG TTATAGATCA 
TACTTAAGAC CAATAACACA AGCCCCTTCT GAGACAGCCA CCGATGCATC CTCTCTTTTT 
GCCCTACAAG CTTTCTTAAG AAGCCGAGAC CGGTCACATC AAAAATTCTA TAACATGATG 
ACCAAAACAC AAATGTTCAT TCGCTTCATT GAGGAATGTT CTTTTGTCAG TGATAAAGAT 
GCAAGCCTGG CATTTTTTGA TGATTGTGTA GATAAAGTGG ATATGGACAA GAGTGGTGAA 
ATGCGACTTA TAGAACTGGA TGAATCTTTC AAAAGTGAAC ATACTGTTTT TGTAACACCA 
CCTGAGATAC CCCATCTACC CAATGGTGAA GAACCCCCTT TACAGTACAG CTATAATGGA 
TTTCCAGTTC TTAGAAACAA TTTATTTGAG AGACCTGAAG GATTTCTACA AACACGAAAG 
AACAAATTGC CTTCAAAATC AAGTAGTCCT AGTAGCCCTT CTCCCATGTT CAGAAGAACA 
AAACAGGAAA TTAAATCAGC CCATAAAATT GCCAAGAGGT ACTCTTCCAT TCCTCAGATG 
TGGTCTAGGT GTCTGCTGCG CCACTGCTAT GGACTGTGGT TCATTTGTCT GCCAGCTTAT 
GTGAAAGTGT GTCATTCAAA AGTCAGGGCA CTGAAGACAG CATATGATGT GCTCAAAAAA 
ATGCAGTCGA AAAAGATGGA TCCACCTGAT GAAGTGTGCT ACCGCATTCT CATGCAGCTC 
TGTGGGCAGT ACGATCAGCC CGTGCTTGCA GTTCGAGTGC TTTTTGAAAT GCAGAAAGCT 
GGTATTGATC CCAATGCCAT TACTTATGGT TATTATAATA AGGCTGTTTT GGAAAGCACC 
TGGCCCTCAA GAAGTCGTAG TGGCTATTTT CTTTGGACAA AAGTAAGAAA CGTTGTTTTA 
GGAGTAGCAC AGTTCAAAAG AGCTTTAAAG AAACAGGCAC ACTTATCACA AACAACTCTC 
TCAGCAGATG GCAGTGACCT GGATGCTGTT AGTCATGGCA GCATGGATAG TGGTCATGGG 
ACACACACTG TGGAGCAGGC ACCTTTTAAT ACGGGTTTAA TCAAAGTATA TGCTACTGAT 
GATAGATCTA GTACAGGTGG CCAGTCTGAT CTTGGATATA ATTCATTATC TAAGGATGAA 
GTTAGAAGAG GGGATATATC TACTGAGGAA ATTCAAGAAG AAAAAGATAA GAAAGGAAGT 
GATTCTAGTT CCCTGTCAGA GAGTGAGAGT ACAAAAGGAA GTGCTGACTG TCTTCCTAAA 
CTCAATTATC AAAGTTCTTC CAGTATAGTT CGCCTCAGTG GTACAAATAA CAATGGTGCT 
GATAAAATAT CAGGAGAAAG CACAGAAAGC ACATCTAAGC TGCTCTTACT ATCATCTTTT 
GAGGGTACAA ATGAAACAGG AAATAGTCAA AATAGATGCT TCAGGAAAAG ACATAAAAGT 
GACAACGAAC CTAATTTGCA ACAGCGACTC TCCTGGGGAA GCAGAAACCG CAATCTTAGT 
GGAGGAGTGC TGATGGGACT TATGCTTAAT AGAATCAACC AGGAAGCAAA CCCTGGAGAC 
ATGGTTGAAA AACTAGGAGC TGATGCAAAA ATTCTTTCAA ACGTTATATC ACAAAGCACA 
AGACCAACTA GTCTTGATAT TGGAAAACCC CCTTTAAGAT CAAAAAGAGA CAGTCTAGAA 
AAGGAATCTA GCGACGATGA TACACCTTTC GATGGTTCTA ATTGTTTGGC AGATAAAGTG 
GATTCTCCTG TTATTTTTGA TTTGGAAGAC TTAGACAGTG AGACAGATGG ATCAAAAGTG 
GGATACATTG CTTCACAAAA TCCCAGAAGG ATTCAGCGTA TGAGCAGCAG CTTCTCGGTA 
AAACCTTCTG AAAAAACGGA TGTTGCCACA GGATTTGATC CCCTCTCTCT TTTGGTTGCT 
GAGACTGAAC AGCAGCAAAA AGAGGAAGAG GAGGAGGATG ATGAAGATAG CAAAAGCGTT 
TCAACACCAT CCGCCAGGCG TGACTTAGCC GAAGAGATTG TGATGTATAT GAATAACATG 
AGCAGTCCTT TGACAAGCCG CACACCAAGC ATCGATTTAC AACGGGCATG TGATGACAAA 
TCAACCAGTA AGAAAAGTCC AACATTTGTC AAGGCATGCA GGAGATCTAG CCTGCCTCCT 
CATTCTCCGA GGCCAGTGAG ACTTACCAAA TCTAAAAGTT ATACAAAAAC TGAGGAGAGA 
CCTCGGGACA GACTCTGGTC TTCTCCAGCC TTCTCACCTA CTTGCCCATT TAGGGAAGAA 
TCTCAAGACG CATTAACCCA TTCATCACCT TCATTTAATT TAGATACACT GCTAGTACCT 
AAACTAGATG TTCTAAGGAA CAGTATGTTC ACTGCTGGGA AAGGAGTTGC CGAGAAAGCC 
AGCAAATGGT ATTCAAGATT CACCATGTAT ACCACATCAT CTAAGGATCA AAGTTCTGAT 
CGTACCAGTC TTTCTTCAGT GGGAGCCCAG GATTCTGAAT CTACCTCTTT AACAGATGAA 
GATGTTTGCC ATGAATTGGA AGGAGCCACT TCCTCCCAAG AGAGCTGTGC TGCTTCAGGG 
AATAAGGGAA TTGATTTCAG CAGAACAAGC CTGGAAAGTT CTGCCTCCTT AGAAGGATCC 
CTGTCAAAGT TTGCCTTACC TGGGAAATCA GAAGTGGCAT CTTCCTGCAA CACAAGTAAT 
ACAAATATCT TCCAGAACTA TGCAATGGAG GTTCTTATCT CAAGTTGCTC TCGGTGTAGA 
ACTTGTGATT GTCTTGTCCA TGATGAGGAA ATCATGGCTG GCTGGACAGC AGATGATTCA 
AATCTCAATA CTACATGCCC GTTTTGTGGC AATCTCTTCT TGCCCTTTCT GAATATTGAA 
ATAAGAGATT TAAGACGACC CGGAAGATAC TTTCTGAAGT CAAGTCCATC TACAGAAAAT 
ATGCATTTTC CATCCTCCCT TTCAAGTCAG ACAAGGCAGT CTTGTATCTC AACATCAGCC 
TCTGGTCTTG ACACGTCTGT TATCTCTGTT CAAGGGAATT TTGATCTCAA TAGCAAATCT 
AAACTGCTGG AAAATACATG TGCCCGAACT ATTCACATCC CTGCTGATAG ATCAAAAACA 
GGTGTGTCAA AATGTCCAAT ATTTCCAATG GCCAGGAGTG TTAGTACTTA TGGCCCCTTG 
GATAAGGAAG ACACTGGAGG CCAGAAGCTC ATTCCTACAG GCAGCCTGCC AACTACTTTA 
CAAGGAGCTA CAGATTCTTT AGGATTAGAG TGGCACCTTC CTAGTCCAGA TCCTATCACA 
GTTCCATATC TTAGTCCTTT AGTGGTATGG AAGGAGCTTG AAAGCTTACT GGAAAATGAA 
GGCGACCATG CAATAACAGT GGCAGACTTT GTGGATCATC ATCCAATCGT GTTTTGGAAC 
CTGGTGTGGT ATTTTAGACG TCTCGACTTA CCCAGTAACT TACCTGGATT GATTCTTTCT 
TCTGAGCACT GTAACAAGTA TTCAAAGATT CCTCGCCACT GTATGTCGGA AGATAGTAAA 
TACGTTTTAA TACAAATGTT ATGGGACAAT ATGAAATTAC ATCAGGATCC GGGACAGCCT 
TTGTACATCC TCTGGAATGC CCACACCCAA AAGTATCCAA TGGTCCATTT ATTGCAAAAG 
GGTGATAATT CATTTAACCA GGAACTGTTG AAAAATATGG TAAAAAGCAT TAAAATGAAT 
GATGTCTATG GACCAATGAG TCAAATTTTA GAGACACTGA ATAAATGTCC TCATTTTAAA 
AGACAGAGGA GTTTATATAG AGAAATACTA TTTCTCTCAC TTGTGGCACT GGGAAGAGAA 
AACATTGATA TAGATGCATT TGATAAAGAA TACAAGATGG CATACGATCG TCTCACACCT 
AGTCAAGTCA AGAGTACACA CAACTGTGAT AGACCACCAA GTACAGGGGT GATGGAATGT 
CGAAAAACCT TTGGAGAACC TTATCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DENND4A ( XM_003433926.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_003433974.1 Canis lupus familiaris DENN/MADD domain containing 4A (DENND4A), transcript variant X4, mRNA.

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CloneID	OCe29021
Clone ID Related Accession (Same CDS sequence)	XM_003433926.3 , XM_003433926.4
Accession Version	XM_003433926.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5727bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	C-myc promoter-binding protein isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726113.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_003433926.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTGAAG ATAAGGGTCC TCGTGTTGCT GACTACTTTG TTGTAGCAGG ATTAACTGAT 
GTTTCAAAGC CTCTTGAAGA AGAAATTCAC TTCAATGATG CCTGTCATAA AGTAGCTAAA 
CCAAAAGAAC CTATCACAGA TGTTTCGGTG ATTATTAAAT CTCTTGGGGA AGAAGTGCCA 
CGGGATTATA TATGTATTGA TGTTACCCCA ACTGGATTGT CGGCTGATCT CAATAATGGA 
AGTCTTGTGG GGCCACAGAT TTACCTTTGC TACAGAAGAG GAAGAGATAA GCCTCCACTT 
ACAGATCTGG GGGTTTTGTA TGACTGGAAA GAAAGATTGA AACAGGGATG TGAAATTATT 
CAGAGTACTC CCTATGGACA CCCTGCAAAT ATTAGTGGCA GTACCTCATC ACAAAAAATT 
TATATAACTT ACCGAAGAGC CTCTGAAAAC ATGACGCAAA ATACGTTGGC TGTCACAGAC 
ATATGTATTA TTATACCCAG TAAAGGAGAG AGTCCACCAC ACACGTTTTG CAAAGTCGAT 
AAGAATCTCA ATAACAGTAT GTGGGGATCT GCAGTGTACT TATGTTATAA AAAATCAGTG 
GCAAAGACTA ACACTATATC CTATAAAGCT GGTCTAATTT GTAGATATCC TCAAGAAGAT 
TACGAATCTT TCTCACTCCC AGAATCTGTT CCCCTATTTT GTTTACCAAT GGGTGCAACT 
ATTGAATGTT GGCCACCAAA TAGCAAATAC CCTCTCCCTG TATTTTCTAC ATTTGTGTTA 
ACTGGAGCCT CAGCTGAAAA GGTCTATGGT GCTGCTATTC AGTTTTATGA ACCATACTCT 
GAGGAGAATC TCACAGAAAA ACAGAGACTT CTTTTGGGTT TAGCATCATC AGCAGATGGG 
AAATCTGATA GTTCTAAAAC AATTCACACT AGTAAATGCA TCTGTCTTCT TTCTCACTGG 
CCTTTTTTTG ATGCATTCAG GAAATTTTTG ACCTTTCTGT ATCGTTACTC TATCTCTGGA 
CCTCATGTCC TTCCAATTGA GAAGCATATT TCTCATTTTA TGCATAAAGT TCCTTTTCCA 
TCTCCTCAGA GGCCACGGAT TTTAGTTCAG TTATCGCCAC ATGATAATCT GATTCTCAGT 
CAACCTGTTT CTTCACCTCT TCCACTAAGT GGTGGCAAAT TTTCAACACT ACTTCAAAAT 
TTAGGCCCTG AAAATGCTGT AACACTACTG GTGTTTGCAG TAACAGAGCA CAAGATTCTC 
ATCCATTCCT TACGGCCTTC CGTGCTTACT AGCGTGACAG AAGCATTAGT GTCTATGATT 
TTCCCTTTCC ACTGGCCGTG CCCATATGTT CCTCTCTGCC CACTGGCTTT AGCAGATGTC 
TTGAGTGCAC CATGTCCATT CATTGTAGGA ATTGATTCAA GATATTTTGA TCTCTATGAC 
CCTCCACCAG ATGTTAGTTG TGTTGACCTA GATACCAACA CTATTTCTCA AACTGGCGAT 
AAGAAAAATG TAGCCTGGAA GATACTTCCA AAGAAACCAT GTAAAAACCT GATGAACACA 
CTGAATAATT TGCACCAGCA GTTGGCAAAA TTGCAGCAGA GACCAAGAGA TGATGGACTA 
ATGGACTTAG CAATGAATGA CTATGATTTT AATTCTGGAA AGAGGTTGCA TATGATAGAT 
TTGGAAATCC AAGAGGCATT TTTGTGTTTC ATGGCCTCTA TTTTAAAAGG TTATAGATCA 
TACTTAAGAC CAATAACACA AGCCCCTTCT GAGACAGCCA CCGATGCATC CTCTCTTTTT 
GCCCTACAAG CTTTCTTAAG AAGCCGAGAC CGGTCACATC AAAAATTCTA TAACATGATG 
ACCAAAACAC AAATGTTCAT TCGCTTCATT GAGGAATGTT CTTTTGTCAG TGATAAAGAT 
GCAAGCCTGG CATTTTTTGA TGATTGTGTA GATAAAGTGG ATATGGACAA GAGTGGTGAA 
ATGCGACTTA TAGAACTGGA TGAATCTTTC AAAAGTGAAC ATACTGTTTT TGTAACACCA 
CCTGAGATAC CCCATCTACC CAATGGTGAA GAACCCCCTT TACAGTACAG CTATAATGGA 
TTTCCAGTTC TTAGAAACAA TTTATTTGAG AGACCTGAAG GATTTCTACA AACACGAAAG 
AACAAATTGC CTTCAAAATC AAGTAGTCCT AGTAGCCCTT CTCCCATGTT CAGAAGAACA 
AAACAGGAAA TTAAATCAGC CCATAAAATT GCCAAGAGGT ACTCTTCCAT TCCTCAGATG 
TGGTCTAGGT GTCTGCTGCG CCACTGCTAT GGACTGTGGT TCATTTGTCT GCCAGCTTAT 
GTGAAAGTGT GTCATTCAAA AGTCAGGGCA CTGAAGACAG CATATGATGT GCTCAAAAAA 
ATGCAGTCGA AAAAGATGGA TCCACCTGAT GAAGTGTGCT ACCGCATTCT CATGCAGCTC 
TGTGGGCAGT ACGATCAGCC CGTGCTTGCA GTTCGAGTGC TTTTTGAAAT GCAGAAAGCT 
GGTATTGATC CCAATGCCAT TACTTATGGT TATTATAATA AGGCTGTTTT GGAAAGCACC 
TGGCCCTCAA GAAGTCGTAG TGGCTATTTT CTTTGGACAA AAGTAAGAAA CGTTGTTTTA 
GGAGTAGCAC AGTTCAAAAG AGCTTTAAAG AAACAGGCAC ACTTATCACA AACAACTCTC 
TCAGATGGCA GTGACCTGGA TGCTGTTAGT CATGGCAGCA TGGATAGTGG TCATGGGACA 
CACACTGTGG AGCAGGCACC TTTTAATACG GGTTTAATCA AAGTATATGC TACTGATGAT 
AGATCTAGTA CAGGTGGCCA GTCTGATCTT GGATATAATT CATTATCTAA GGATGAAGTT 
AGAAGAGGGG ATATATCTAC TGAGGAAATT CAAGAAGAAA AAGATAAGAA AGGAAGTGAT 
TCTAGTTCCC TGTCAGAGAG TGAGAGTACA AAAGGAAGTG CTGACTGTCT TCCTAAACTC 
AATTATCAAA GTTCTTCCAG TATAGTTCGC CTCAGTGGTA CAAATAACAA TGGTGCTGAT 
AAAATATCAG GAGAAAGCAC AGAAAGCACA TCTAAGCTGC TCTTACTATC ATCTTTTGAG 
GGTACAAATG AAACAGGAAA TAGTCAAAAT AGATGCTTCA GGAAAAGACA TAAAAGTGAC 
AACGAACCTA ATTTGCAACA GCGACTCTCC TGGGGAAGCA GAAACCGCAA TCTTAGTGGA 
GGAGTGCTGA TGGGACTTAT GCTTAATAGA ATCAACCAGG AAGCAAACCC TGGAGACATG 
GTTGAAAAAC TAGGAGCTGA TGCAAAAATT CTTTCAAACG TTATATCACA AAGCACAAGA 
CCAACTAGTC TTGATATTGG AAAACCCCCT TTAAGATCAA AAAGAGACAG TCTAGAAAAG 
GAATCTAGCG ACGATGATAC ACCTTTCGAT GGTTCTAATT GTTTGGCAGA TAAAGTGGAT 
TCTCCTGTTA TTTTTGATTT GGAAGACTTA GACAGTGAGA CAGATGGATC AAAAGTGGGA 
TACATTGCTT CACAAAATCC CAGAAGGATT CAGCGTATGA GCAGCAGCTT CTCGGTAAAA 
CCTTCTGAAA AAACGGATGT TGCCACAGGA TTTGATCCCC TCTCTCTTTT GGTTGCTGAG 
ACTGAACAGC AGCAAAAAGA GGAAGAGGAG GAGGATGATG AAGATAGCAA AAGCGTTTCA 
ACACCATCCG CCAGGCGTGA CTTAGCCGAA GAGATTGTGA TGTATATGAA TAACATGAGC 
AGTCCTTTGA CAAGCCGCAC ACCAAGCATC GATTTACAAC GGGCATGTGA TGACAAATCA 
ACCAGTAAGA AAAGTCCAAC ATTTGTCAAG GCATGCAGGA GATCTAGCCT GCCTCCTCAT 
TCTCCGAGGC CAGTGAGACT TACCAAATCT AAAAGTTATA CAAAAACTGA GGAGAGACCT 
CGGGACAGAC TCTGGTCTTC TCCAGCCTTC TCACCTACTT GCCCATTTAG GGAAGAATCT 
CAAGACGCAT TAACCCATTC ATCACCTTCA TTTAATTTAG ATACACTGCT AGTACCTAAA 
CTAGATGTTC TAAGGAACAG TATGTTCACT GCTGGGAAAG GAGTTGCCGA GAAAGCCAGC 
AAATGGTATT CAAGATTCAC CATGTATACC ACATCATCTA AGGATCAAAG TTCTGATCGT 
ACCAGTCTTT CTTCAGTGGG AGCCCAGGAT TCTGAATCTA CCTCTTTAAC AGATGAAGAT 
GTTTGCCATG AATTGGAAGG AGCCACTTCC TCCCAAGAGA GCTGTGCTGC TTCAGGGAAT 
AAGGGAATTG ATTTCAGCAG AACAAGCCTG GAAAGTTCTG CCTCCTTAGA AGGATCCCTG 
TCAAAGTTTG CCTTACCTGG GAAATCAGAA GTGGCATCTT CCTGCAACAC AAGTAATACA 
AATATCTTCC AGAACTATGC AATGGAGGTT CTTATCTCAA GTTGCTCTCG GTGTAGAACT 
TGTGATTGTC TTGTCCATGA TGAGGAAATC ATGGCTGGCT GGACAGCAGA TGATTCAAAT 
CTCAATACTA CATGCCCGTT TTGTGGCAAT CTCTTCTTGC CCTTTCTGAA TATTGAAATA 
AGAGATTTAA GACGACCCGG AAGATACTTT CTGAAGTCAA GTCCATCTAC AGAAAATATG 
CATTTTCCAT CCTCCCTTTC AAGTCAGACA AGGCAGTCTT GTATCTCAAC ATCAGCCTCT 
GGTCTTGACA CGTCTGTTAT CTCTGTTCAA GGGAATTTTG ATCTCAATAG CAAATCTAAA 
CTGCTGGAAA ATACATGTGC CCGAACTATT CACATCCCTG CTGATAGATC AAAAACAGGT 
GTGTCAAAAT GTCCAATATT TCCAATGGCC AGGAGTGTTA GTACTTATGG CCCCTTGGAT 
AAGGAAGACA CTGGAGGCCA GAAGCTCATT CCTACAGGCA GCCTGCCAAC TACTTTACAA 
GGAGCTACAG ATTCTTTAGG ATTAGAGTGG CACCTTCCTA GTCCAGATCC TATCACAGTT 
CCATATCTTA GTCCTTTAGT GGTATGGAAG GAGCTTGAAA GCTTACTGGA AAATGAAGGC 
GACCATGCAA TAACAGTGGC AGACTTTGTG GATCATCATC CAATCGTGTT TTGGAACCTG 
GTGTGGTATT TTAGACGTCT CGACTTACCC AGTAACTTAC CTGGATTGAT TCTTTCTTCT 
GAGCACTGTA ACAAGTATTC AAAGATTCCT CGCCACTGTA TGTCGGAAGA TAGTAAATAC 
GTTTTAATAC AAATGTTATG GGACAATATG AAATTACATC AGGATCCGGG ACAGCCTTTG 
TACATCCTCT GGAATGCCCA CACCCAAAAG TATCCAATGG TCCATTTATT GCAAAAGGGT 
GATAATTCAT TTAACCAGGA ACTGTTGAAA AATATGGTAA AAAGCATTAA AATGAATGAT 
GTCTATGGAC CAATGAGTCA AATTTTAGAG ACACTGAATA AATGTCCTCA TTTTAAAAGA 
CAGAGGAGTT TATATAGAGA AATACTATTT CTCTCACTTG TGGCACTGGG AAGAGAAAAC 
ATTGATATAG ATGCATTTGA TAAAGAATAC AAGATGGCAT ACGATCGTCT CACACCTAGT 
CAAGTCAAGA GTACACACAA CTGTGATAGA CCACCAAGTA CAGGGGTGAT GGAATGTCGA 
AAAACCTTTG GAGAACCTTA TCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003433974.1
CDS	378..6104
Translation	MIEDKGPRVADYFVVAGLTDVSKPLEEEIHFNDACHKVAKPKEPITDVSVIIKSLGEEVPRDYICIDVTPTGLSADLNNGSLVGPQIYLCYRRGRDKPPLTDLGVLYDWKERLKQGCEIIQSTPYGHPANISGSTSSQKIYITYRRASENMTQNTLAVTDICIIIPSKGESPPHTFCKVDKNLNNSMWGSAVYLCYKKSVAKTNTISYKAGLICRYPQEDYESFSLPESVPLFCLPMGATIECWPPNSKYPLPVFSTFVLTGASAEKVYGAAIQFYEPYSEENLTEKQRLLLGLASSADGKSDSSKTIHTSKCICLLSHWPFFDAFRKFLTFLYRYSISGPHVLPIEKHISHFMHKVPFPSPQRPRILVQLSPHDNLILSQPVSSPLPLSGGKFSTLLQNLGPENAVTLLVFAVTEHKILIHSLRPSVLTSVTEALVSMIFPFHWPCPYVPLCPLALADVLSAPCPFIVGIDSRYFDLYDPPPDVSCVDLDTNTISQTGDKKNVAWKILPKKPCKNLMNTLNNLHQQLAKLQQRPRDDGLMDLAMNDYDFNSGKRLHMIDLEIQEAFLCFMASILKGYRSYLRPITQAPSETATDASSLFALQAFLRSRDRSHQKFYNMMTKTQMFIRFIEECSFVSDKDASLAFFDDCVDKVDMDKSGEMRLIELDESFKSEHTVFVTPPEIPHLPNGEEPPLQYSYNGFPVLRNNLFERPEGFLQTRKNKLPSKSSSPSSPSPMFRRTKQEIKSAHKIAKRYSSIPQMWSRCLLRHCYGLWFICLPAYVKVCHSKVRALKTAYDVLKKMQSKKMDPPDEVCYRILMQLCGQYDQPVLAVRVLFEMQKAGIDPNAITYGYYNKAVLESTWPSRSRSGYFLWTKVRNVVLGVAQFKRALKKQAHLSQTTLSDGSDLDAVSHGSMDSGHGTHTVEQAPFNTGLIKVYATDDRSSTGGQSDLGYNSLSKDEVRRGDISTEEIQEEKDKKGSDSSSLSESESTKGSADCLPKLNYQSSSSIVRLSGTNNNGADKISGESTESTSKLLLLSSFEGTNETGNSQNRCFRKRHKSDNEPNLQQRLSWGSRNRNLSGGVLMGLMLNRINQEANPGDMVEKLGADAKILSNVISQSTRPTSLDIGKPPLRSKRDSLEKESSDDDTPFDGSNCLADKVDSPVIFDLEDLDSETDGSKVGYIASQNPRRIQRMSSSFSVKPSEKTDVATGFDPLSLLVAETEQQQKEEEEEDDEDSKSVSTPSARRDLAEEIVMYMNNMSSPLTSRTPSIDLQRACDDKSTSKKSPTFVKACRRSSLPPHSPRPVRLTKSKSYTKTEERPRDRLWSSPAFSPTCPFREESQDALTHSSPSFNLDTLLVPKLDVLRNSMFTAGKGVAEKASKWYSRFTMYTTSSKDQSSDRTSLSSVGAQDSESTSLTDEDVCHELEGATSSQESCAASGNKGIDFSRTSLESSASLEGSLSKFALPGKSEVASSCNTSNTNIFQNYAMEVLISSCSRCRTCDCLVHDEEIMAGWTADDSNLNTTCPFCGNLFLPFLNIEIRDLRRPGRYFLKSSPSTENMHFPSSLSSQTRQSCISTSASGLDTSVISVQGNFDLNSKSKLLENTCARTIHIPADRSKTGVSKCPIFPMARSVSTYGPLDKEDTGGQKLIPTGSLPTTLQGATDSLGLEWHLPSPDPITVPYLSPLVVWKELESLLENEGDHAITVADFVDHHPIVFWNLVWYFRRLDLPSNLPGLILSSEHCNKYSKIPRHCMSEDSKYVLIQMLWDNMKLHQDPGQPLYILWNAHTQKYPMVHLLQKGDNSFNQELLKNMVKSIKMNDVYGPMSQILETLNKCPHFKRQRSLYREILFLSLVALGRENIDIDAFDKEYKMAYDRLTPSQVKSTHNCDRPPSTGVMECRKTFGEPYL

Target ORF information:

RefSeq Version	XM_003433926.3
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris DENN/MADD domain containing 4A (DENND4A), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003433926.3

ORF Insert Sequence:

ATGATTGAAG ATAAGGGTCC TCGTGTTGCT GACTACTTTG TTGTAGCAGG ATTAACTGAT 
GTTTCAAAGC CTCTTGAAGA AGAAATTCAC TTCAATGATG CCTGTCATAA AGTAGCTAAA 
CCAAAAGAAC CTATCACAGA TGTTTCGGTG ATTATTAAAT CTCTTGGGGA AGAAGTGCCA 
CGGGATTATA TATGTATTGA TGTTACCCCA ACTGGATTGT CGGCTGATCT CAATAATGGA 
AGTCTTGTGG GGCCACAGAT TTACCTTTGC TACAGAAGAG GAAGAGATAA GCCTCCACTT 
ACAGATCTGG GGGTTTTGTA TGACTGGAAA GAAAGATTGA AACAGGGATG TGAAATTATT 
CAGAGTACTC CCTATGGACA CCCTGCAAAT ATTAGTGGCA GTACCTCATC ACAAAAAATT 
TATATAACTT ACCGAAGAGC CTCTGAAAAC ATGACGCAAA ATACGTTGGC TGTCACAGAC 
ATATGTATTA TTATACCCAG TAAAGGAGAG AGTCCACCAC ACACGTTTTG CAAAGTCGAT 
AAGAATCTCA ATAACAGTAT GTGGGGATCT GCAGTGTACT TATGTTATAA AAAATCAGTG 
GCAAAGACTA ACACTATATC CTATAAAGCT GGTCTAATTT GTAGATATCC TCAAGAAGAT 
TACGAATCTT TCTCACTCCC AGAATCTGTT CCCCTATTTT GTTTACCAAT GGGTGCAACT 
ATTGAATGTT GGCCACCAAA TAGCAAATAC CCTCTCCCTG TATTTTCTAC ATTTGTGTTA 
ACTGGAGCCT CAGCTGAAAA GGTCTATGGT GCTGCTATTC AGTTTTATGA ACCATACTCT 
GAGGAGAATC TCACAGAAAA ACAGAGACTT CTTTTGGGTT TAGCATCATC AGCAGATGGG 
AAATCTGATA GTTCTAAAAC AATTCACACT AGTAAATGCA TCTGTCTTCT TTCTCACTGG 
CCTTTTTTTG ATGCATTCAG GAAATTTTTG ACCTTTCTGT ATCGTTACTC TATCTCTGGA 
CCTCATGTCC TTCCAATTGA GAAGCATATT TCTCATTTTA TGCATAAAGT TCCTTTTCCA 
TCTCCTCAGA GGCCACGGAT TTTAGTTCAG TTATCGCCAC ATGATAATCT GATTCTCAGT 
CAACCTGTTT CTTCACCTCT TCCACTAAGT GGTGGCAAAT TTTCAACACT ACTTCAAAAT 
TTAGGCCCTG AAAATGCTGT AACACTACTG GTGTTTGCAG TAACAGAGCA CAAGATTCTC 
ATCCATTCCT TACGGCCTTC CGTGCTTACT AGCGTGACAG AAGCATTAGT GTCTATGATT 
TTCCCTTTCC ACTGGCCGTG CCCATATGTT CCTCTCTGCC CACTGGCTTT AGCAGATGTC 
TTGAGTGCAC CATGTCCATT CATTGTAGGA ATTGATTCAA GATATTTTGA TCTCTATGAC 
CCTCCACCAG ATGTTAGTTG TGTTGACCTA GATACCAACA CTATTTCTCA AACTGGCGAT 
AAGAAAAATG TAGCCTGGAA GATACTTCCA AAGAAACCAT GTAAAAACCT GATGAACACA 
CTGAATAATT TGCACCAGCA GTTGGCAAAA TTGCAGCAGA GACCAAGAGA TGATGGACTA 
ATGGACTTAG CAATGAATGA CTATGATTTT AATTCTGGAA AGAGGTTGCA TATGATAGAT 
TTGGAAATCC AAGAGGCATT TTTGTGTTTC ATGGCCTCTA TTTTAAAAGG TTATAGATCA 
TACTTAAGAC CAATAACACA AGCCCCTTCT GAGACAGCCA CCGATGCATC CTCTCTTTTT 
GCCCTACAAG CTTTCTTAAG AAGCCGAGAC CGGTCACATC AAAAATTCTA TAACATGATG 
ACCAAAACAC AAATGTTCAT TCGCTTCATT GAGGAATGTT CTTTTGTCAG TGATAAAGAT 
GCAAGCCTGG CATTTTTTGA TGATTGTGTA GATAAAGTGG ATATGGACAA GAGTGGTGAA 
ATGCGACTTA TAGAACTGGA TGAATCTTTC AAAAGTGAAC ATACTGTTTT TGTAACACCA 
CCTGAGATAC CCCATCTACC CAATGGTGAA GAACCCCCTT TACAGTACAG CTATAATGGA 
TTTCCAGTTC TTAGAAACAA TTTATTTGAG AGACCTGAAG GATTTCTACA AACACGAAAG 
AACAAATTGC CTTCAAAATC AAGTAGTCCT AGTAGCCCTT CTCCCATGTT CAGAAGAACA 
AAACAGGAAA TTAAATCAGC CCATAAAATT GCCAAGAGGT ACTCTTCCAT TCCTCAGATG 
TGGTCTAGGT GTCTGCTGCG CCACTGCTAT GGACTGTGGT TCATTTGTCT GCCAGCTTAT 
GTGAAAGTGT GTCATTCAAA AGTCAGGGCA CTGAAGACAG CATATGATGT GCTCAAAAAA 
ATGCAGTCGA AAAAGATGGA TCCACCTGAT GAAGTGTGCT ACCGCATTCT CATGCAGCTC 
TGTGGGCAGT ACGATCAGCC CGTGCTTGCA GTTCGAGTGC TTTTTGAAAT GCAGAAAGCT 
GGTATTGATC CCAATGCCAT TACTTATGGT TATTATAATA AGGCTGTTTT GGAAAGCACC 
TGGCCCTCAA GAAGTCGTAG TGGCTATTTT CTTTGGACAA AAGTAAGAAA CGTTGTTTTA 
GGAGTAGCAC AGTTCAAAAG AGCTTTAAAG AAACAGGCAC ACTTATCACA AACAACTCTC 
TCAGATGGCA GTGACCTGGA TGCTGTTAGT CATGGCAGCA TGGATAGTGG TCATGGGACA 
CACACTGTGG AGCAGGCACC TTTTAATACG GGTTTAATCA AAGTATATGC TACTGATGAT 
AGATCTAGTA CAGGTGGCCA GTCTGATCTT GGATATAATT CATTATCTAA GGATGAAGTT 
AGAAGAGGGG ATATATCTAC TGAGGAAATT CAAGAAGAAA AAGATAAGAA AGGAAGTGAT 
TCTAGTTCCC TGTCAGAGAG TGAGAGTACA AAAGGAAGTG CTGACTGTCT TCCTAAACTC 
AATTATCAAA GTTCTTCCAG TATAGTTCGC CTCAGTGGTA CAAATAACAA TGGTGCTGAT 
AAAATATCAG GAGAAAGCAC AGAAAGCACA TCTAAGCTGC TCTTACTATC ATCTTTTGAG 
GGTACAAATG AAACAGGAAA TAGTCAAAAT AGATGCTTCA GGAAAAGACA TAAAAGTGAC 
AACGAACCTA ATTTGCAACA GCGACTCTCC TGGGGAAGCA GAAACCGCAA TCTTAGTGGA 
GGAGTGCTGA TGGGACTTAT GCTTAATAGA ATCAACCAGG AAGCAAACCC TGGAGACATG 
GTTGAAAAAC TAGGAGCTGA TGCAAAAATT CTTTCAAACG TTATATCACA AAGCACAAGA 
CCAACTAGTC TTGATATTGG AAAACCCCCT TTAAGATCAA AAAGAGACAG TCTAGAAAAG 
GAATCTAGCG ACGATGATAC ACCTTTCGAT GGTTCTAATT GTTTGGCAGA TAAAGTGGAT 
TCTCCTGTTA TTTTTGATTT GGAAGACTTA GACAGTGAGA CAGATGGATC AAAAGTGGGA 
TACATTGCTT CACAAAATCC CAGAAGGATT CAGCGTATGA GCAGCAGCTT CTCGGTAAAA 
CCTTCTGAAA AAACGGATGT TGCCACAGGA TTTGATCCCC TCTCTCTTTT GGTTGCTGAG 
ACTGAACAGC AGCAAAAAGA GGAAGAGGAG GAGGATGATG AAGATAGCAA AAGCGTTTCA 
ACACCATCCG CCAGGCGTGA CTTAGCCGAA GAGATTGTGA TGTATATGAA TAACATGAGC 
AGTCCTTTGA CAAGCCGCAC ACCAAGCATC GATTTACAAC GGGCATGTGA TGACAAATCA 
ACCAGTAAGA AAAGTCCAAC ATTTGTCAAG GCATGCAGGA GATCTAGCCT GCCTCCTCAT 
TCTCCGAGGC CAGTGAGACT TACCAAATCT AAAAGTTATA CAAAAACTGA GGAGAGACCT 
CGGGACAGAC TCTGGTCTTC TCCAGCCTTC TCACCTACTT GCCCATTTAG GGAAGAATCT 
CAAGACGCAT TAACCCATTC ATCACCTTCA TTTAATTTAG ATACACTGCT AGTACCTAAA 
CTAGATGTTC TAAGGAACAG TATGTTCACT GCTGGGAAAG GAGTTGCCGA GAAAGCCAGC 
AAATGGTATT CAAGATTCAC CATGTATACC ACATCATCTA AGGATCAAAG TTCTGATCGT 
ACCAGTCTTT CTTCAGTGGG AGCCCAGGAT TCTGAATCTA CCTCTTTAAC AGATGAAGAT 
GTTTGCCATG AATTGGAAGG AGCCACTTCC TCCCAAGAGA GCTGTGCTGC TTCAGGGAAT 
AAGGGAATTG ATTTCAGCAG AACAAGCCTG GAAAGTTCTG CCTCCTTAGA AGGATCCCTG 
TCAAAGTTTG CCTTACCTGG GAAATCAGAA GTGGCATCTT CCTGCAACAC AAGTAATACA 
AATATCTTCC AGAACTATGC AATGGAGGTT CTTATCTCAA GTTGCTCTCG GTGTAGAACT 
TGTGATTGTC TTGTCCATGA TGAGGAAATC ATGGCTGGCT GGACAGCAGA TGATTCAAAT 
CTCAATACTA CATGCCCGTT TTGTGGCAAT CTCTTCTTGC CCTTTCTGAA TATTGAAATA 
AGAGATTTAA GACGACCCGG AAGATACTTT CTGAAGTCAA GTCCATCTAC AGAAAATATG 
CATTTTCCAT CCTCCCTTTC AAGTCAGACA AGGCAGTCTT GTATCTCAAC ATCAGCCTCT 
GGTCTTGACA CGTCTGTTAT CTCTGTTCAA GGGAATTTTG ATCTCAATAG CAAATCTAAA 
CTGCTGGAAA ATACATGTGC CCGAACTATT CACATCCCTG CTGATAGATC AAAAACAGGT 
GTGTCAAAAT GTCCAATATT TCCAATGGCC AGGAGTGTTA GTACTTATGG CCCCTTGGAT 
AAGGAAGACA CTGGAGGCCA GAAGCTCATT CCTACAGGCA GCCTGCCAAC TACTTTACAA 
GGAGCTACAG ATTCTTTAGG ATTAGAGTGG CACCTTCCTA GTCCAGATCC TATCACAGTT 
CCATATCTTA GTCCTTTAGT GGTATGGAAG GAGCTTGAAA GCTTACTGGA AAATGAAGGC 
GACCATGCAA TAACAGTGGC AGACTTTGTG GATCATCATC CAATCGTGTT TTGGAACCTG 
GTGTGGTATT TTAGACGTCT CGACTTACCC AGTAACTTAC CTGGATTGAT TCTTTCTTCT 
GAGCACTGTA ACAAGTATTC AAAGATTCCT CGCCACTGTA TGTCGGAAGA TAGTAAATAC 
GTTTTAATAC AAATGTTATG GGACAATATG AAATTACATC AGGATCCGGG ACAGCCTTTG 
TACATCCTCT GGAATGCCCA CACCCAAAAG TATCCAATGG TCCATTTATT GCAAAAGGGT 
GATAATTCAT TTAACCAGGA ACTGTTGAAA AATATGGTAA AAAGCATTAA AATGAATGAT 
GTCTATGGAC CAATGAGTCA AATTTTAGAG ACACTGAATA AATGTCCTCA TTTTAAAAGA 
CAGAGGAGTT TATATAGAGA AATACTATTT CTCTCACTTG TGGCACTGGG AAGAGAAAAC 
ATTGATATAG ATGCATTTGA TAAAGAATAC AAGATGGCAT ACGATCGTCT CACACCTAGT 
CAAGTCAAGA GTACACACAA CTGTGATAGA CCACCAAGTA CAGGGGTGAT GGAATGTCGA 
AAAACCTTTG GAGAACCTTA TCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DENND4A ( XM_003433926.4 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_003433974.1 Canis lupus familiaris DENN domain containing 4A (DENND4A), transcript variant X6, mRNA.

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CloneID	OCe29021
Clone ID Related Accession (Same CDS sequence)	XM_003433926.3 , XM_003433926.4
Accession Version	XM_003433926.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5727bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	C-myc promoter-binding protein isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006612.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_003433926.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTGAAG ATAAGGGTCC TCGTGTTGCT GACTACTTTG TTGTAGCAGG ATTAACTGAT 
GTTTCAAAGC CTCTTGAAGA AGAAATTCAC TTCAATGATG CCTGTCATAA AGTAGCTAAA 
CCAAAAGAAC CTATCACAGA TGTTTCGGTG ATTATTAAAT CTCTTGGGGA AGAAGTGCCA 
CGGGATTATA TATGTATTGA TGTTACCCCA ACTGGATTGT CGGCTGATCT CAATAATGGA 
AGTCTTGTGG GGCCACAGAT TTACCTTTGC TACAGAAGAG GAAGAGATAA GCCTCCACTT 
ACAGATCTGG GGGTTTTGTA TGACTGGAAA GAAAGATTGA AACAGGGATG TGAAATTATT 
CAGAGTACTC CCTATGGACA CCCTGCAAAT ATTAGTGGCA GTACCTCATC ACAAAAAATT 
TATATAACTT ACCGAAGAGC CTCTGAAAAC ATGACGCAAA ATACGTTGGC TGTCACAGAC 
ATATGTATTA TTATACCCAG TAAAGGAGAG AGTCCACCAC ACACGTTTTG CAAAGTCGAT 
AAGAATCTCA ATAACAGTAT GTGGGGATCT GCAGTGTACT TATGTTATAA AAAATCAGTG 
GCAAAGACTA ACACTATATC CTATAAAGCT GGTCTAATTT GTAGATATCC TCAAGAAGAT 
TACGAATCTT TCTCACTCCC AGAATCTGTT CCCCTATTTT GTTTACCAAT GGGTGCAACT 
ATTGAATGTT GGCCACCAAA TAGCAAATAC CCTCTCCCTG TATTTTCTAC ATTTGTGTTA 
ACTGGAGCCT CAGCTGAAAA GGTCTATGGT GCTGCTATTC AGTTTTATGA ACCATACTCT 
GAGGAGAATC TCACAGAAAA ACAGAGACTT CTTTTGGGTT TAGCATCATC AGCAGATGGG 
AAATCTGATA GTTCTAAAAC AATTCACACT AGTAAATGCA TCTGTCTTCT TTCTCACTGG 
CCTTTTTTTG ATGCATTCAG GAAATTTTTG ACCTTTCTGT ATCGTTACTC TATCTCTGGA 
CCTCATGTCC TTCCAATTGA GAAGCATATT TCTCATTTTA TGCATAAAGT TCCTTTTCCA 
TCTCCTCAGA GGCCACGGAT TTTAGTTCAG TTATCGCCAC ATGATAATCT GATTCTCAGT 
CAACCTGTTT CTTCACCTCT TCCACTAAGT GGTGGCAAAT TTTCAACACT ACTTCAAAAT 
TTAGGCCCTG AAAATGCTGT AACACTACTG GTGTTTGCAG TAACAGAGCA CAAGATTCTC 
ATCCATTCCT TACGGCCTTC CGTGCTTACT AGCGTGACAG AAGCATTAGT GTCTATGATT 
TTCCCTTTCC ACTGGCCGTG CCCATATGTT CCTCTCTGCC CACTGGCTTT AGCAGATGTC 
TTGAGTGCAC CATGTCCATT CATTGTAGGA ATTGATTCAA GATATTTTGA TCTCTATGAC 
CCTCCACCAG ATGTTAGTTG TGTTGACCTA GATACCAACA CTATTTCTCA AACTGGCGAT 
AAGAAAAATG TAGCCTGGAA GATACTTCCA AAGAAACCAT GTAAAAACCT GATGAACACA 
CTGAATAATT TGCACCAGCA GTTGGCAAAA TTGCAGCAGA GACCAAGAGA TGATGGACTA 
ATGGACTTAG CAATGAATGA CTATGATTTT AATTCTGGAA AGAGGTTGCA TATGATAGAT 
TTGGAAATCC AAGAGGCATT TTTGTGTTTC ATGGCCTCTA TTTTAAAAGG TTATAGATCA 
TACTTAAGAC CAATAACACA AGCCCCTTCT GAGACAGCCA CCGATGCATC CTCTCTTTTT 
GCCCTACAAG CTTTCTTAAG AAGCCGAGAC CGGTCACATC AAAAATTCTA TAACATGATG 
ACCAAAACAC AAATGTTCAT TCGCTTCATT GAGGAATGTT CTTTTGTCAG TGATAAAGAT 
GCAAGCCTGG CATTTTTTGA TGATTGTGTA GATAAAGTGG ATATGGACAA GAGTGGTGAA 
ATGCGACTTA TAGAACTGGA TGAATCTTTC AAAAGTGAAC ATACTGTTTT TGTAACACCA 
CCTGAGATAC CCCATCTACC CAATGGTGAA GAACCCCCTT TACAGTACAG CTATAATGGA 
TTTCCAGTTC TTAGAAACAA TTTATTTGAG AGACCTGAAG GATTTCTACA AACACGAAAG 
AACAAATTGC CTTCAAAATC AAGTAGTCCT AGTAGCCCTT CTCCCATGTT CAGAAGAACA 
AAACAGGAAA TTAAATCAGC CCATAAAATT GCCAAGAGGT ACTCTTCCAT TCCTCAGATG 
TGGTCTAGGT GTCTGCTGCG CCACTGCTAT GGACTGTGGT TCATTTGTCT GCCAGCTTAT 
GTGAAAGTGT GTCATTCAAA AGTCAGGGCA CTGAAGACAG CATATGATGT GCTCAAAAAA 
ATGCAGTCGA AAAAGATGGA TCCACCTGAT GAAGTGTGCT ACCGCATTCT CATGCAGCTC 
TGTGGGCAGT ACGATCAGCC CGTGCTTGCA GTTCGAGTGC TTTTTGAAAT GCAGAAAGCT 
GGTATTGATC CCAATGCCAT TACTTATGGT TATTATAATA AGGCTGTTTT GGAAAGCACC 
TGGCCCTCAA GAAGTCGTAG TGGCTATTTT CTTTGGACAA AAGTAAGAAA CGTTGTTTTA 
GGAGTAGCAC AGTTCAAAAG AGCTTTAAAG AAACAGGCAC ACTTATCACA AACAACTCTC 
TCAGATGGCA GTGACCTGGA TGCTGTTAGT CATGGCAGCA TGGATAGTGG TCATGGGACA 
CACACTGTGG AGCAGGCACC TTTTAATACG GGTTTAATCA AAGTATATGC TACTGATGAT 
AGATCTAGTA CAGGTGGCCA GTCTGATCTT GGATATAATT CATTATCTAA GGATGAAGTT 
AGAAGAGGGG ATATATCTAC TGAGGAAATT CAAGAAGAAA AAGATAAGAA AGGAAGTGAT 
TCTAGTTCCC TGTCAGAGAG TGAGAGTACA AAAGGAAGTG CTGACTGTCT TCCTAAACTC 
AATTATCAAA GTTCTTCCAG TATAGTTCGC CTCAGTGGTA CAAATAACAA TGGTGCTGAT 
AAAATATCAG GAGAAAGCAC AGAAAGCACA TCTAAGCTGC TCTTACTATC ATCTTTTGAG 
GGTACAAATG AAACAGGAAA TAGTCAAAAT AGATGCTTCA GGAAAAGACA TAAAAGTGAC 
AACGAACCTA ATTTGCAACA GCGACTCTCC TGGGGAAGCA GAAACCGCAA TCTTAGTGGA 
GGAGTGCTGA TGGGACTTAT GCTTAATAGA ATCAACCAGG AAGCAAACCC TGGAGACATG 
GTTGAAAAAC TAGGAGCTGA TGCAAAAATT CTTTCAAACG TTATATCACA AAGCACAAGA 
CCAACTAGTC TTGATATTGG AAAACCCCCT TTAAGATCAA AAAGAGACAG TCTAGAAAAG 
GAATCTAGCG ACGATGATAC ACCTTTCGAT GGTTCTAATT GTTTGGCAGA TAAAGTGGAT 
TCTCCTGTTA TTTTTGATTT GGAAGACTTA GACAGTGAGA CAGATGGATC AAAAGTGGGA 
TACATTGCTT CACAAAATCC CAGAAGGATT CAGCGTATGA GCAGCAGCTT CTCGGTAAAA 
CCTTCTGAAA AAACGGATGT TGCCACAGGA TTTGATCCCC TCTCTCTTTT GGTTGCTGAG 
ACTGAACAGC AGCAAAAAGA GGAAGAGGAG GAGGATGATG AAGATAGCAA AAGCGTTTCA 
ACACCATCCG CCAGGCGTGA CTTAGCCGAA GAGATTGTGA TGTATATGAA TAACATGAGC 
AGTCCTTTGA CAAGCCGCAC ACCAAGCATC GATTTACAAC GGGCATGTGA TGACAAATCA 
ACCAGTAAGA AAAGTCCAAC ATTTGTCAAG GCATGCAGGA GATCTAGCCT GCCTCCTCAT 
TCTCCGAGGC CAGTGAGACT TACCAAATCT AAAAGTTATA CAAAAACTGA GGAGAGACCT 
CGGGACAGAC TCTGGTCTTC TCCAGCCTTC TCACCTACTT GCCCATTTAG GGAAGAATCT 
CAAGACGCAT TAACCCATTC ATCACCTTCA TTTAATTTAG ATACACTGCT AGTACCTAAA 
CTAGATGTTC TAAGGAACAG TATGTTCACT GCTGGGAAAG GAGTTGCCGA GAAAGCCAGC 
AAATGGTATT CAAGATTCAC CATGTATACC ACATCATCTA AGGATCAAAG TTCTGATCGT 
ACCAGTCTTT CTTCAGTGGG AGCCCAGGAT TCTGAATCTA CCTCTTTAAC AGATGAAGAT 
GTTTGCCATG AATTGGAAGG AGCCACTTCC TCCCAAGAGA GCTGTGCTGC TTCAGGGAAT 
AAGGGAATTG ATTTCAGCAG AACAAGCCTG GAAAGTTCTG CCTCCTTAGA AGGATCCCTG 
TCAAAGTTTG CCTTACCTGG GAAATCAGAA GTGGCATCTT CCTGCAACAC AAGTAATACA 
AATATCTTCC AGAACTATGC AATGGAGGTT CTTATCTCAA GTTGCTCTCG GTGTAGAACT 
TGTGATTGTC TTGTCCATGA TGAGGAAATC ATGGCTGGCT GGACAGCAGA TGATTCAAAT 
CTCAATACTA CATGCCCGTT TTGTGGCAAT CTCTTCTTGC CCTTTCTGAA TATTGAAATA 
AGAGATTTAA GACGACCCGG AAGATACTTT CTGAAGTCAA GTCCATCTAC AGAAAATATG 
CATTTTCCAT CCTCCCTTTC AAGTCAGACA AGGCAGTCTT GTATCTCAAC ATCAGCCTCT 
GGTCTTGACA CGTCTGTTAT CTCTGTTCAA GGGAATTTTG ATCTCAATAG CAAATCTAAA 
CTGCTGGAAA ATACATGTGC CCGAACTATT CACATCCCTG CTGATAGATC AAAAACAGGT 
GTGTCAAAAT GTCCAATATT TCCAATGGCC AGGAGTGTTA GTACTTATGG CCCCTTGGAT 
AAGGAAGACA CTGGAGGCCA GAAGCTCATT CCTACAGGCA GCCTGCCAAC TACTTTACAA 
GGAGCTACAG ATTCTTTAGG ATTAGAGTGG CACCTTCCTA GTCCAGATCC TATCACAGTT 
CCATATCTTA GTCCTTTAGT GGTATGGAAG GAGCTTGAAA GCTTACTGGA AAATGAAGGC 
GACCATGCAA TAACAGTGGC AGACTTTGTG GATCATCATC CAATCGTGTT TTGGAACCTG 
GTGTGGTATT TTAGACGTCT CGACTTACCC AGTAACTTAC CTGGATTGAT TCTTTCTTCT 
GAGCACTGTA ACAAGTATTC AAAGATTCCT CGCCACTGTA TGTCGGAAGA TAGTAAATAC 
GTTTTAATAC AAATGTTATG GGACAATATG AAATTACATC AGGATCCGGG ACAGCCTTTG 
TACATCCTCT GGAATGCCCA CACCCAAAAG TATCCAATGG TCCATTTATT GCAAAAGGGT 
GATAATTCAT TTAACCAGGA ACTGTTGAAA AATATGGTAA AAAGCATTAA AATGAATGAT 
GTCTATGGAC CAATGAGTCA AATTTTAGAG ACACTGAATA AATGTCCTCA TTTTAAAAGA 
CAGAGGAGTT TATATAGAGA AATACTATTT CTCTCACTTG TGGCACTGGG AAGAGAAAAC 
ATTGATATAG ATGCATTTGA TAAAGAATAC AAGATGGCAT ACGATCGTCT CACACCTAGT 
CAAGTCAAGA GTACACACAA CTGTGATAGA CCACCAAGTA CAGGGGTGAT GGAATGTCGA 
AAAACCTTTG GAGAACCTTA TCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003433974.1
CDS	343..6069
Translation	MIEDKGPRVADYFVVAGLTDVSKPLEEEIHFNDACHKVAKPKEPITDVSVIIKSLGEEVPRDYICIDVTPTGLSADLNNGSLVGPQIYLCYRRGRDKPPLTDLGVLYDWKERLKQGCEIIQSTPYGHPANISGSTSSQKIYITYRRASENMTQNTLAVTDICIIIPSKGESPPHTFCKVDKNLNNSMWGSAVYLCYKKSVAKTNTISYKAGLICRYPQEDYESFSLPESVPLFCLPMGATIECWPPNSKYPLPVFSTFVLTGASAEKVYGAAIQFYEPYSEENLTEKQRLLLGLASSADGKSDSSKTIHTSKCICLLSHWPFFDAFRKFLTFLYRYSISGPHVLPIEKHISHFMHKVPFPSPQRPRILVQLSPHDNLILSQPVSSPLPLSGGKFSTLLQNLGPENAVTLLVFAVTEHKILIHSLRPSVLTSVTEALVSMIFPFHWPCPYVPLCPLALADVLSAPCPFIVGIDSRYFDLYDPPPDVSCVDLDTNTISQTGDKKNVAWKILPKKPCKNLMNTLNNLHQQLAKLQQRPRDDGLMDLAMNDYDFNSGKRLHMIDLEIQEAFLCFMASILKGYRSYLRPITQAPSETATDASSLFALQAFLRSRDRSHQKFYNMMTKTQMFIRFIEECSFVSDKDASLAFFDDCVDKVDMDKSGEMRLIELDESFKSEHTVFVTPPEIPHLPNGEEPPLQYSYNGFPVLRNNLFERPEGFLQTRKNKLPSKSSSPSSPSPMFRRTKQEIKSAHKIAKRYSSIPQMWSRCLLRHCYGLWFICLPAYVKVCHSKVRALKTAYDVLKKMQSKKMDPPDEVCYRILMQLCGQYDQPVLAVRVLFEMQKAGIDPNAITYGYYNKAVLESTWPSRSRSGYFLWTKVRNVVLGVAQFKRALKKQAHLSQTTLSDGSDLDAVSHGSMDSGHGTHTVEQAPFNTGLIKVYATDDRSSTGGQSDLGYNSLSKDEVRRGDISTEEIQEEKDKKGSDSSSLSESESTKGSADCLPKLNYQSSSSIVRLSGTNNNGADKISGESTESTSKLLLLSSFEGTNETGNSQNRCFRKRHKSDNEPNLQQRLSWGSRNRNLSGGVLMGLMLNRINQEANPGDMVEKLGADAKILSNVISQSTRPTSLDIGKPPLRSKRDSLEKESSDDDTPFDGSNCLADKVDSPVIFDLEDLDSETDGSKVGYIASQNPRRIQRMSSSFSVKPSEKTDVATGFDPLSLLVAETEQQQKEEEEEDDEDSKSVSTPSARRDLAEEIVMYMNNMSSPLTSRTPSIDLQRACDDKSTSKKSPTFVKACRRSSLPPHSPRPVRLTKSKSYTKTEERPRDRLWSSPAFSPTCPFREESQDALTHSSPSFNLDTLLVPKLDVLRNSMFTAGKGVAEKASKWYSRFTMYTTSSKDQSSDRTSLSSVGAQDSESTSLTDEDVCHELEGATSSQESCAASGNKGIDFSRTSLESSASLEGSLSKFALPGKSEVASSCNTSNTNIFQNYAMEVLISSCSRCRTCDCLVHDEEIMAGWTADDSNLNTTCPFCGNLFLPFLNIEIRDLRRPGRYFLKSSPSTENMHFPSSLSSQTRQSCISTSASGLDTSVISVQGNFDLNSKSKLLENTCARTIHIPADRSKTGVSKCPIFPMARSVSTYGPLDKEDTGGQKLIPTGSLPTTLQGATDSLGLEWHLPSPDPITVPYLSPLVVWKELESLLENEGDHAITVADFVDHHPIVFWNLVWYFRRLDLPSNLPGLILSSEHCNKYSKIPRHCMSEDSKYVLIQMLWDNMKLHQDPGQPLYILWNAHTQKYPMVHLLQKGDNSFNQELLKNMVKSIKMNDVYGPMSQILETLNKCPHFKRQRSLYREILFLSLVALGRENIDIDAFDKEYKMAYDRLTPSQVKSTHNCDRPPSTGVMECRKTFGEPYL

Target ORF information:

RefSeq Version	XM_003433926.4
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris DENN domain containing 4A (DENND4A), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003433926.4

ORF Insert Sequence:

ATGATTGAAG ATAAGGGTCC TCGTGTTGCT GACTACTTTG TTGTAGCAGG ATTAACTGAT 
GTTTCAAAGC CTCTTGAAGA AGAAATTCAC TTCAATGATG CCTGTCATAA AGTAGCTAAA 
CCAAAAGAAC CTATCACAGA TGTTTCGGTG ATTATTAAAT CTCTTGGGGA AGAAGTGCCA 
CGGGATTATA TATGTATTGA TGTTACCCCA ACTGGATTGT CGGCTGATCT CAATAATGGA 
AGTCTTGTGG GGCCACAGAT TTACCTTTGC TACAGAAGAG GAAGAGATAA GCCTCCACTT 
ACAGATCTGG GGGTTTTGTA TGACTGGAAA GAAAGATTGA AACAGGGATG TGAAATTATT 
CAGAGTACTC CCTATGGACA CCCTGCAAAT ATTAGTGGCA GTACCTCATC ACAAAAAATT 
TATATAACTT ACCGAAGAGC CTCTGAAAAC ATGACGCAAA ATACGTTGGC TGTCACAGAC 
ATATGTATTA TTATACCCAG TAAAGGAGAG AGTCCACCAC ACACGTTTTG CAAAGTCGAT 
AAGAATCTCA ATAACAGTAT GTGGGGATCT GCAGTGTACT TATGTTATAA AAAATCAGTG 
GCAAAGACTA ACACTATATC CTATAAAGCT GGTCTAATTT GTAGATATCC TCAAGAAGAT 
TACGAATCTT TCTCACTCCC AGAATCTGTT CCCCTATTTT GTTTACCAAT GGGTGCAACT 
ATTGAATGTT GGCCACCAAA TAGCAAATAC CCTCTCCCTG TATTTTCTAC ATTTGTGTTA 
ACTGGAGCCT CAGCTGAAAA GGTCTATGGT GCTGCTATTC AGTTTTATGA ACCATACTCT 
GAGGAGAATC TCACAGAAAA ACAGAGACTT CTTTTGGGTT TAGCATCATC AGCAGATGGG 
AAATCTGATA GTTCTAAAAC AATTCACACT AGTAAATGCA TCTGTCTTCT TTCTCACTGG 
CCTTTTTTTG ATGCATTCAG GAAATTTTTG ACCTTTCTGT ATCGTTACTC TATCTCTGGA 
CCTCATGTCC TTCCAATTGA GAAGCATATT TCTCATTTTA TGCATAAAGT TCCTTTTCCA 
TCTCCTCAGA GGCCACGGAT TTTAGTTCAG TTATCGCCAC ATGATAATCT GATTCTCAGT 
CAACCTGTTT CTTCACCTCT TCCACTAAGT GGTGGCAAAT TTTCAACACT ACTTCAAAAT 
TTAGGCCCTG AAAATGCTGT AACACTACTG GTGTTTGCAG TAACAGAGCA CAAGATTCTC 
ATCCATTCCT TACGGCCTTC CGTGCTTACT AGCGTGACAG AAGCATTAGT GTCTATGATT 
TTCCCTTTCC ACTGGCCGTG CCCATATGTT CCTCTCTGCC CACTGGCTTT AGCAGATGTC 
TTGAGTGCAC CATGTCCATT CATTGTAGGA ATTGATTCAA GATATTTTGA TCTCTATGAC 
CCTCCACCAG ATGTTAGTTG TGTTGACCTA GATACCAACA CTATTTCTCA AACTGGCGAT 
AAGAAAAATG TAGCCTGGAA GATACTTCCA AAGAAACCAT GTAAAAACCT GATGAACACA 
CTGAATAATT TGCACCAGCA GTTGGCAAAA TTGCAGCAGA GACCAAGAGA TGATGGACTA 
ATGGACTTAG CAATGAATGA CTATGATTTT AATTCTGGAA AGAGGTTGCA TATGATAGAT 
TTGGAAATCC AAGAGGCATT TTTGTGTTTC ATGGCCTCTA TTTTAAAAGG TTATAGATCA 
TACTTAAGAC CAATAACACA AGCCCCTTCT GAGACAGCCA CCGATGCATC CTCTCTTTTT 
GCCCTACAAG CTTTCTTAAG AAGCCGAGAC CGGTCACATC AAAAATTCTA TAACATGATG 
ACCAAAACAC AAATGTTCAT TCGCTTCATT GAGGAATGTT CTTTTGTCAG TGATAAAGAT 
GCAAGCCTGG CATTTTTTGA TGATTGTGTA GATAAAGTGG ATATGGACAA GAGTGGTGAA 
ATGCGACTTA TAGAACTGGA TGAATCTTTC AAAAGTGAAC ATACTGTTTT TGTAACACCA 
CCTGAGATAC CCCATCTACC CAATGGTGAA GAACCCCCTT TACAGTACAG CTATAATGGA 
TTTCCAGTTC TTAGAAACAA TTTATTTGAG AGACCTGAAG GATTTCTACA AACACGAAAG 
AACAAATTGC CTTCAAAATC AAGTAGTCCT AGTAGCCCTT CTCCCATGTT CAGAAGAACA 
AAACAGGAAA TTAAATCAGC CCATAAAATT GCCAAGAGGT ACTCTTCCAT TCCTCAGATG 
TGGTCTAGGT GTCTGCTGCG CCACTGCTAT GGACTGTGGT TCATTTGTCT GCCAGCTTAT 
GTGAAAGTGT GTCATTCAAA AGTCAGGGCA CTGAAGACAG CATATGATGT GCTCAAAAAA 
ATGCAGTCGA AAAAGATGGA TCCACCTGAT GAAGTGTGCT ACCGCATTCT CATGCAGCTC 
TGTGGGCAGT ACGATCAGCC CGTGCTTGCA GTTCGAGTGC TTTTTGAAAT GCAGAAAGCT 
GGTATTGATC CCAATGCCAT TACTTATGGT TATTATAATA AGGCTGTTTT GGAAAGCACC 
TGGCCCTCAA GAAGTCGTAG TGGCTATTTT CTTTGGACAA AAGTAAGAAA CGTTGTTTTA 
GGAGTAGCAC AGTTCAAAAG AGCTTTAAAG AAACAGGCAC ACTTATCACA AACAACTCTC 
TCAGATGGCA GTGACCTGGA TGCTGTTAGT CATGGCAGCA TGGATAGTGG TCATGGGACA 
CACACTGTGG AGCAGGCACC TTTTAATACG GGTTTAATCA AAGTATATGC TACTGATGAT 
AGATCTAGTA CAGGTGGCCA GTCTGATCTT GGATATAATT CATTATCTAA GGATGAAGTT 
AGAAGAGGGG ATATATCTAC TGAGGAAATT CAAGAAGAAA AAGATAAGAA AGGAAGTGAT 
TCTAGTTCCC TGTCAGAGAG TGAGAGTACA AAAGGAAGTG CTGACTGTCT TCCTAAACTC 
AATTATCAAA GTTCTTCCAG TATAGTTCGC CTCAGTGGTA CAAATAACAA TGGTGCTGAT 
AAAATATCAG GAGAAAGCAC AGAAAGCACA TCTAAGCTGC TCTTACTATC ATCTTTTGAG 
GGTACAAATG AAACAGGAAA TAGTCAAAAT AGATGCTTCA GGAAAAGACA TAAAAGTGAC 
AACGAACCTA ATTTGCAACA GCGACTCTCC TGGGGAAGCA GAAACCGCAA TCTTAGTGGA 
GGAGTGCTGA TGGGACTTAT GCTTAATAGA ATCAACCAGG AAGCAAACCC TGGAGACATG 
GTTGAAAAAC TAGGAGCTGA TGCAAAAATT CTTTCAAACG TTATATCACA AAGCACAAGA 
CCAACTAGTC TTGATATTGG AAAACCCCCT TTAAGATCAA AAAGAGACAG TCTAGAAAAG 
GAATCTAGCG ACGATGATAC ACCTTTCGAT GGTTCTAATT GTTTGGCAGA TAAAGTGGAT 
TCTCCTGTTA TTTTTGATTT GGAAGACTTA GACAGTGAGA CAGATGGATC AAAAGTGGGA 
TACATTGCTT CACAAAATCC CAGAAGGATT CAGCGTATGA GCAGCAGCTT CTCGGTAAAA 
CCTTCTGAAA AAACGGATGT TGCCACAGGA TTTGATCCCC TCTCTCTTTT GGTTGCTGAG 
ACTGAACAGC AGCAAAAAGA GGAAGAGGAG GAGGATGATG AAGATAGCAA AAGCGTTTCA 
ACACCATCCG CCAGGCGTGA CTTAGCCGAA GAGATTGTGA TGTATATGAA TAACATGAGC 
AGTCCTTTGA CAAGCCGCAC ACCAAGCATC GATTTACAAC GGGCATGTGA TGACAAATCA 
ACCAGTAAGA AAAGTCCAAC ATTTGTCAAG GCATGCAGGA GATCTAGCCT GCCTCCTCAT 
TCTCCGAGGC CAGTGAGACT TACCAAATCT AAAAGTTATA CAAAAACTGA GGAGAGACCT 
CGGGACAGAC TCTGGTCTTC TCCAGCCTTC TCACCTACTT GCCCATTTAG GGAAGAATCT 
CAAGACGCAT TAACCCATTC ATCACCTTCA TTTAATTTAG ATACACTGCT AGTACCTAAA 
CTAGATGTTC TAAGGAACAG TATGTTCACT GCTGGGAAAG GAGTTGCCGA GAAAGCCAGC 
AAATGGTATT CAAGATTCAC CATGTATACC ACATCATCTA AGGATCAAAG TTCTGATCGT 
ACCAGTCTTT CTTCAGTGGG AGCCCAGGAT TCTGAATCTA CCTCTTTAAC AGATGAAGAT 
GTTTGCCATG AATTGGAAGG AGCCACTTCC TCCCAAGAGA GCTGTGCTGC TTCAGGGAAT 
AAGGGAATTG ATTTCAGCAG AACAAGCCTG GAAAGTTCTG CCTCCTTAGA AGGATCCCTG 
TCAAAGTTTG CCTTACCTGG GAAATCAGAA GTGGCATCTT CCTGCAACAC AAGTAATACA 
AATATCTTCC AGAACTATGC AATGGAGGTT CTTATCTCAA GTTGCTCTCG GTGTAGAACT 
TGTGATTGTC TTGTCCATGA TGAGGAAATC ATGGCTGGCT GGACAGCAGA TGATTCAAAT 
CTCAATACTA CATGCCCGTT TTGTGGCAAT CTCTTCTTGC CCTTTCTGAA TATTGAAATA 
AGAGATTTAA GACGACCCGG AAGATACTTT CTGAAGTCAA GTCCATCTAC AGAAAATATG 
CATTTTCCAT CCTCCCTTTC AAGTCAGACA AGGCAGTCTT GTATCTCAAC ATCAGCCTCT 
GGTCTTGACA CGTCTGTTAT CTCTGTTCAA GGGAATTTTG ATCTCAATAG CAAATCTAAA 
CTGCTGGAAA ATACATGTGC CCGAACTATT CACATCCCTG CTGATAGATC AAAAACAGGT 
GTGTCAAAAT GTCCAATATT TCCAATGGCC AGGAGTGTTA GTACTTATGG CCCCTTGGAT 
AAGGAAGACA CTGGAGGCCA GAAGCTCATT CCTACAGGCA GCCTGCCAAC TACTTTACAA 
GGAGCTACAG ATTCTTTAGG ATTAGAGTGG CACCTTCCTA GTCCAGATCC TATCACAGTT 
CCATATCTTA GTCCTTTAGT GGTATGGAAG GAGCTTGAAA GCTTACTGGA AAATGAAGGC 
GACCATGCAA TAACAGTGGC AGACTTTGTG GATCATCATC CAATCGTGTT TTGGAACCTG 
GTGTGGTATT TTAGACGTCT CGACTTACCC AGTAACTTAC CTGGATTGAT TCTTTCTTCT 
GAGCACTGTA ACAAGTATTC AAAGATTCCT CGCCACTGTA TGTCGGAAGA TAGTAAATAC 
GTTTTAATAC AAATGTTATG GGACAATATG AAATTACATC AGGATCCGGG ACAGCCTTTG 
TACATCCTCT GGAATGCCCA CACCCAAAAG TATCCAATGG TCCATTTATT GCAAAAGGGT 
GATAATTCAT TTAACCAGGA ACTGTTGAAA AATATGGTAA AAAGCATTAA AATGAATGAT 
GTCTATGGAC CAATGAGTCA AATTTTAGAG ACACTGAATA AATGTCCTCA TTTTAAAAGA 
CAGAGGAGTT TATATAGAGA AATACTATTT CTCTCACTTG TGGCACTGGG AAGAGAAAAC 
ATTGATATAG ATGCATTTGA TAAAGAATAC AAGATGGCAT ACGATCGTCT CACACCTAGT 
CAAGTCAAGA GTACACACAA CTGTGATAGA CCACCAAGTA CAGGGGTGAT GGAATGTCGA 
AAAACCTTTG GAGAACCTTA TCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DENND4A ( XM_005638530.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005638587.1 Canis lupus familiaris DENN/MADD domain containing 4A (DENND4A), transcript variant X5, mRNA.

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CloneID	OCe29022
Clone ID Related Accession (Same CDS sequence)	XM_005638530.2 , XM_005638530.3
Accession Version	XM_005638530.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5625bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	C-myc promoter-binding protein isoform X5
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726113.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005638530.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTGAAG ATAAGGGTCC TCGTGTTGCT GACTACTTTG TTGTAGCAGG ATTAACTGAT 
GTTTCAAAGC CTCTTGAAGA AGAAATTCAC TTCAATGATG CCTGTCATAA AGTAGCTAAA 
CCAAAAGAAC CTATCACAGA TGTTTCGGTG ATTATTAAAT CTCTTGGGGA AGAAGTGCCA 
CGGGATTATA TATGTATTGA TGTTACCCCA ACTGGATTGT CGGCTGATCT CAATAATGGA 
AGTCTTGTGG GGCCACAGAT TTACCTTTGC TACAGAAGAG GAAGAGATAA GCCTCCACTT 
ACAGATCTGG GGGTTTTGTA TGACTGGAAA GAAAGATTGA AACAGGGATG TGAAATTATT 
CAGAGTACTC CCTATGGACA CCCTGCAAAT ATTAGTGGCA GTACCTCATC ACAAAAAATT 
TATATAACTT ACCGAAGAGC CTCTGAAAAC ATGACGCAAA ATACGTTGGC TGTCACAGAC 
ATATGTATTA TTATACCCAG TAAAGGAGAG AGTCCACCAC ACACGTTTTG CAAAGTCGAT 
AAGAATCTCA ATAACAGTAT GTGGGGATCT GCAGTGTACT TATGTTATAA AAAATCAGTG 
GCAAAGACTA ACACTATATC CTATAAAGCT GGTCTAATTT GTAGATATCC TCAAGAAGAT 
TACGAATCTT TCTCACTCCC AGAATCTGTT CCCCTATTTT GTTTACCAAT GGGTGCAACT 
ATTGAATGTT GGCCACCAAA TAGCAAATAC CCTCTCCCTG TATTTTCTAC ATTTGTGTTA 
ACTGGAGCCT CAGCTGAAAA GGTCTATGGT GCTGCTATTC AGTTTTATGA ACCATACTCT 
GAGGAGAATC TCACAGAAAA ACAGAGACTT CTTTTGGGTT TAGCATCATC AGCAGATGGG 
AAATCTGATA GTTCTAAAAC AATTCACACT AGTAAATGCA TCTGTCTTCT TTCTCACTGG 
CCTTTTTTTG ATGCATTCAG GAAATTTTTG ACCTTTCTGT ATCGTTACTC TATCTCTGGA 
CCTCATGTCC TTCCAATTGA GAAGCATATT TCTCATTTTA TGCATAAAGT TCCTTTTCCA 
TCTCCTCAGA GGCCACGGAT TTTAGTTCAG TTATCGCCAC ATGATAATCT GATTCTCAGT 
CAACCTGTTT CTTCACCTCT TCCACTAAGT GGTGGCAAAT TTTCAACACT ACTTCAAAAT 
TTAGGCCCTG AAAATGCTGT AACACTACTG GTGTTTGCAG TAACAGAGCA CAAGATTCTC 
ATCCATTCCT TACGGCCTTC CGTGCTTACT AGCGTGACAG AAGCATTAGT GTCTATGATT 
TTCCCTTTCC ACTGGCCGTG CCCATATGTT CCTCTCTGCC CACTGGCTTT AGCAGATGTC 
TTGAGTGCAC CATGTCCATT CATTGTAGGA ATTGATTCAA GATATTTTGA TCTCTATGAC 
CCTCCACCAG ATGTTAGTTG TGTTGACCTA GATACCAACA CTATTTCTCA AACTGGCGAT 
AAGAAAAATG TAGCCTGGAA GATACTTCCA AAGAAACCAT GTAAAAACCT GATGAACACA 
CTGAATAATT TGCACCAGCA GTTGGCAAAA TTGCAGCAGA GACCAAGAGA TGATGGACTA 
ATGGACTTAG CAATGAATGA CTATGATTTT AATTCTGGAA AGAGGTTGCA TATGATAGAT 
TTGGAAATCC AAGAGGCATT TTTGTGTTTC ATGGCCTCTA TTTTAAAAGG TTATAGATCA 
TACTTAAGAC CAATAACACA AGCCCCTTCT GAGACAGCCA CCGATGCATC CTCTCTTTTT 
GCCCTACAAG CTTTCTTAAG AAGCCGAGAC CGGTCACATC AAAAATTCTA TAACATGATG 
ACCAAAACAC AAATGTTCAT TCGCTTCATT GAGGAATGTT CTTTTGTCAG TGATAAAGAT 
GCAAGCCTGG CATTTTTTGA TGATTGTGTA GATAAAGTGG ATATGGACAA GAGTGGTGAA 
ATGCGACTTA TAGAACTGGA TGAATCTTTC AAAAGTGAAC ATACTGTTTT TGTAACACCA 
CCTGAGATAC CCCATCTACC CAATGGTGAA GAACCCCCTT TACAGTACAG CTATAATGGA 
TTTCCAGTTC TTAGAAACAA TTTATTTGAG AGACCTGAAG GATTTCTACA AACACGAAAG 
AACAAATTGC CTTCAAAATC AAGTAGTCCT AGTAGCCCTT CTCCCATGTT CAGAAGAACA 
AAACAGGAAA TTAAATCAGC CCATAAAATT GCCAAGAGGT ACTCTTCCAT TCCTCAGATG 
TGGTCTAGGT GTCTGCTGCG CCACTGCTAT GGACTGTGGT TCATTTGTCT GCCAGCTTAT 
GTGAAAGTGT GTCATTCAAA AGTCAGGGCA CTGAAGACAG CATATGATGT GCTCAAAAAA 
ATGCAGTCGA AAAAGATGGA TCCACCTGAT GAAGTGTGCT ACCGCATTCT CATGCAGCTC 
TGTGGGCAGT ACGATCAGCC CGTGCTTGCA GTTCGAGTGC TTTTTGAAAT GCAGAAAGCT 
GGTATTGATC CCAATGCCAT TACTTATGGT TATTATAATA AGGCTGTTTT GGAAAGCACC 
TGGCCCTCAA GAAGTCGTAG TGGCTATTTT CTTTGGACAA AAGTAAGAAA CGTTGTTTTA 
GGAGTAGCAC AGTTCAAAAG AGCTTTAAAG AAACAGGCAC ACTTATCACA AACAACTCTC 
TCAGGTGGCC AGTCTGATCT TGGATATAAT TCATTATCTA AGGATGAAGT TAGAAGAGGG 
GATATATCTA CTGAGGAAAT TCAAGAAGAA AAAGATAAGA AAGGAAGTGA TTCTAGTTCC 
CTGTCAGAGA GTGAGAGTAC AAAAGGAAGT GCTGACTGTC TTCCTAAACT CAATTATCAA 
AGTTCTTCCA GTATAGTTCG CCTCAGTGGT ACAAATAACA ATGGTGCTGA TAAAATATCA 
GGAGAAAGCA CAGAAAGCAC ATCTAAGCTG CTCTTACTAT CATCTTTTGA GGGTACAAAT 
GAAACAGGAA ATAGTCAAAA TAGATGCTTC AGGAAAAGAC ATAAAAGTGA CAACGAACCT 
AATTTGCAAC AGCGACTCTC CTGGGGAAGC AGAAACCGCA ATCTTAGTGG AGGAGTGCTG 
ATGGGACTTA TGCTTAATAG AATCAACCAG GAAGCAAACC CTGGAGACAT GGTTGAAAAA 
CTAGGAGCTG ATGCAAAAAT TCTTTCAAAC GTTATATCAC AAAGCACAAG ACCAACTAGT 
CTTGATATTG GAAAACCCCC TTTAAGATCA AAAAGAGACA GTCTAGAAAA GGAATCTAGC 
GACGATGATA CACCTTTCGA TGGTTCTAAT TGTTTGGCAG ATAAAGTGGA TTCTCCTGTT 
ATTTTTGATT TGGAAGACTT AGACAGTGAG ACAGATGGAT CAAAAGTGGG ATACATTGCT 
TCACAAAATC CCAGAAGGAT TCAGCGTATG AGCAGCAGCT TCTCGGTAAA ACCTTCTGAA 
AAAACGGATG TTGCCACAGG ATTTGATCCC CTCTCTCTTT TGGTTGCTGA GACTGAACAG 
CAGCAAAAAG AGGAAGAGGA GGAGGATGAT GAAGATAGCA AAAGCGTTTC AACACCATCC 
GCCAGGCGTG ACTTAGCCGA AGAGATTGTG ATGTATATGA ATAACATGAG CAGTCCTTTG 
ACAAGCCGCA CACCAAGCAT CGATTTACAA CGGGCATGTG ATGACAAATC AACCAGTAAG 
AAAAGTCCAA CATTTGTCAA GGCATGCAGG AGATCTAGCC TGCCTCCTCA TTCTCCGAGG 
CCAGTGAGAC TTACCAAATC TAAAAGTTAT ACAAAAACTG AGGAGAGACC TCGGGACAGA 
CTCTGGTCTT CTCCAGCCTT CTCACCTACT TGCCCATTTA GGGAAGAATC TCAAGACGCA 
TTAACCCATT CATCACCTTC ATTTAATTTA GATACACTGC TAGTACCTAA ACTAGATGTT 
CTAAGGAACA GTATGTTCAC TGCTGGGAAA GGAGTTGCCG AGAAAGCCAG CAAATGGTAT 
TCAAGATTCA CCATGTATAC CACATCATCT AAGGATCAAA GTTCTGATCG TACCAGTCTT 
TCTTCAGTGG GAGCCCAGGA TTCTGAATCT ACCTCTTTAA CAGATGAAGA TGTTTGCCAT 
GAATTGGAAG GAGCCACTTC CTCCCAAGAG AGCTGTGCTG CTTCAGGGAA TAAGGGAATT 
GATTTCAGCA GAACAAGCCT GGAAAGTTCT GCCTCCTTAG AAGGATCCCT GTCAAAGTTT 
GCCTTACCTG GGAAATCAGA AGTGGCATCT TCCTGCAACA CAAGTAATAC AAATATCTTC 
CAGAACTATG CAATGGAGGT TCTTATCTCA AGTTGCTCTC GGTGTAGAAC TTGTGATTGT 
CTTGTCCATG ATGAGGAAAT CATGGCTGGC TGGACAGCAG ATGATTCAAA TCTCAATACT 
ACATGCCCGT TTTGTGGCAA TCTCTTCTTG CCCTTTCTGA ATATTGAAAT AAGAGATTTA 
AGACGACCCG GAAGATACTT TCTGAAGTCA AGTCCATCTA CAGAAAATAT GCATTTTCCA 
TCCTCCCTTT CAAGTCAGAC AAGGCAGTCT TGTATCTCAA CATCAGCCTC TGGTCTTGAC 
ACGTCTGTTA TCTCTGTTCA AGGGAATTTT GATCTCAATA GCAAATCTAA ACTGCTGGAA 
AATACATGTG CCCGAACTAT TCACATCCCT GCTGATAGAT CAAAAACAGG TGTGTCAAAA 
TGTCCAATAT TTCCAATGGC CAGGAGTGTT AGTACTTATG GCCCCTTGGA TAAGGAAGAC 
ACTGGAGGCC AGAAGCTCAT TCCTACAGGC AGCCTGCCAA CTACTTTACA AGGAGCTACA 
GATTCTTTAG GATTAGAGTG GCACCTTCCT AGTCCAGATC CTATCACAGT TCCATATCTT 
AGTCCTTTAG TGGTATGGAA GGAGCTTGAA AGCTTACTGG AAAATGAAGG CGACCATGCA 
ATAACAGTGG CAGACTTTGT GGATCATCAT CCAATCGTGT TTTGGAACCT GGTGTGGTAT 
TTTAGACGTC TCGACTTACC CAGTAACTTA CCTGGATTGA TTCTTTCTTC TGAGCACTGT 
AACAAGTATT CAAAGATTCC TCGCCACTGT ATGTCGGAAG ATAGTAAATA CGTTTTAATA 
CAAATGTTAT GGGACAATAT GAAATTACAT CAGGATCCGG GACAGCCTTT GTACATCCTC 
TGGAATGCCC ACAGTCAAAA TCGTACTCTT TTGTTTGAGA CCCAAAAGTA TCCAATGGTC 
CATTTATTGC AAAAGGGTGA TAATTCATTT AACCAGGAAC TGTTGAAAAA TATGGTAAAA 
AGCATTAAAA TGAATGATGT CTATGGACCA ATGAGTCAAA TTTTAGAGAC ACTGAATAAA 
TGTCCTCATT TTAAAAGACA GAGGAGTTTA TATAGAGAAA TACTATTTCT CTCACTTGTG 
GCACTGGGAA GAGAAAACAT TGATATAGAT GCATTTGATA AAGAATACAA GATGGCATAC 
GATCGTCTCA CACCTAGTCA AGTCAAGAGT ACACACAACT GTGATAGACC ACCAAGTACA 
GGGGTGATGG AATGTCGAAA AACCTTTGGA GAACCTTATC TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005638587.1
CDS	378..6002
Translation	MIEDKGPRVADYFVVAGLTDVSKPLEEEIHFNDACHKVAKPKEPITDVSVIIKSLGEEVPRDYICIDVTPTGLSADLNNGSLVGPQIYLCYRRGRDKPPLTDLGVLYDWKERLKQGCEIIQSTPYGHPANISGSTSSQKIYITYRRASENMTQNTLAVTDICIIIPSKGESPPHTFCKVDKNLNNSMWGSAVYLCYKKSVAKTNTISYKAGLICRYPQEDYESFSLPESVPLFCLPMGATIECWPPNSKYPLPVFSTFVLTGASAEKVYGAAIQFYEPYSEENLTEKQRLLLGLASSADGKSDSSKTIHTSKCICLLSHWPFFDAFRKFLTFLYRYSISGPHVLPIEKHISHFMHKVPFPSPQRPRILVQLSPHDNLILSQPVSSPLPLSGGKFSTLLQNLGPENAVTLLVFAVTEHKILIHSLRPSVLTSVTEALVSMIFPFHWPCPYVPLCPLALADVLSAPCPFIVGIDSRYFDLYDPPPDVSCVDLDTNTISQTGDKKNVAWKILPKKPCKNLMNTLNNLHQQLAKLQQRPRDDGLMDLAMNDYDFNSGKRLHMIDLEIQEAFLCFMASILKGYRSYLRPITQAPSETATDASSLFALQAFLRSRDRSHQKFYNMMTKTQMFIRFIEECSFVSDKDASLAFFDDCVDKVDMDKSGEMRLIELDESFKSEHTVFVTPPEIPHLPNGEEPPLQYSYNGFPVLRNNLFERPEGFLQTRKNKLPSKSSSPSSPSPMFRRTKQEIKSAHKIAKRYSSIPQMWSRCLLRHCYGLWFICLPAYVKVCHSKVRALKTAYDVLKKMQSKKMDPPDEVCYRILMQLCGQYDQPVLAVRVLFEMQKAGIDPNAITYGYYNKAVLESTWPSRSRSGYFLWTKVRNVVLGVAQFKRALKKQAHLSQTTLSGGQSDLGYNSLSKDEVRRGDISTEEIQEEKDKKGSDSSSLSESESTKGSADCLPKLNYQSSSSIVRLSGTNNNGADKISGESTESTSKLLLLSSFEGTNETGNSQNRCFRKRHKSDNEPNLQQRLSWGSRNRNLSGGVLMGLMLNRINQEANPGDMVEKLGADAKILSNVISQSTRPTSLDIGKPPLRSKRDSLEKESSDDDTPFDGSNCLADKVDSPVIFDLEDLDSETDGSKVGYIASQNPRRIQRMSSSFSVKPSEKTDVATGFDPLSLLVAETEQQQKEEEEEDDEDSKSVSTPSARRDLAEEIVMYMNNMSSPLTSRTPSIDLQRACDDKSTSKKSPTFVKACRRSSLPPHSPRPVRLTKSKSYTKTEERPRDRLWSSPAFSPTCPFREESQDALTHSSPSFNLDTLLVPKLDVLRNSMFTAGKGVAEKASKWYSRFTMYTTSSKDQSSDRTSLSSVGAQDSESTSLTDEDVCHELEGATSSQESCAASGNKGIDFSRTSLESSASLEGSLSKFALPGKSEVASSCNTSNTNIFQNYAMEVLISSCSRCRTCDCLVHDEEIMAGWTADDSNLNTTCPFCGNLFLPFLNIEIRDLRRPGRYFLKSSPSTENMHFPSSLSSQTRQSCISTSASGLDTSVISVQGNFDLNSKSKLLENTCARTIHIPADRSKTGVSKCPIFPMARSVSTYGPLDKEDTGGQKLIPTGSLPTTLQGATDSLGLEWHLPSPDPITVPYLSPLVVWKELESLLENEGDHAITVADFVDHHPIVFWNLVWYFRRLDLPSNLPGLILSSEHCNKYSKIPRHCMSEDSKYVLIQMLWDNMKLHQDPGQPLYILWNAHSQNRTLLFETQKYPMVHLLQKGDNSFNQELLKNMVKSIKMNDVYGPMSQILETLNKCPHFKRQRSLYREILFLSLVALGRENIDIDAFDKEYKMAYDRLTPSQVKSTHNCDRPPSTGVMECRKTFGEPYL

Target ORF information:

RefSeq Version	XM_005638530.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris DENN/MADD domain containing 4A (DENND4A), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005638530.2

ORF Insert Sequence:

ATGATTGAAG ATAAGGGTCC TCGTGTTGCT GACTACTTTG TTGTAGCAGG ATTAACTGAT 
GTTTCAAAGC CTCTTGAAGA AGAAATTCAC TTCAATGATG CCTGTCATAA AGTAGCTAAA 
CCAAAAGAAC CTATCACAGA TGTTTCGGTG ATTATTAAAT CTCTTGGGGA AGAAGTGCCA 
CGGGATTATA TATGTATTGA TGTTACCCCA ACTGGATTGT CGGCTGATCT CAATAATGGA 
AGTCTTGTGG GGCCACAGAT TTACCTTTGC TACAGAAGAG GAAGAGATAA GCCTCCACTT 
ACAGATCTGG GGGTTTTGTA TGACTGGAAA GAAAGATTGA AACAGGGATG TGAAATTATT 
CAGAGTACTC CCTATGGACA CCCTGCAAAT ATTAGTGGCA GTACCTCATC ACAAAAAATT 
TATATAACTT ACCGAAGAGC CTCTGAAAAC ATGACGCAAA ATACGTTGGC TGTCACAGAC 
ATATGTATTA TTATACCCAG TAAAGGAGAG AGTCCACCAC ACACGTTTTG CAAAGTCGAT 
AAGAATCTCA ATAACAGTAT GTGGGGATCT GCAGTGTACT TATGTTATAA AAAATCAGTG 
GCAAAGACTA ACACTATATC CTATAAAGCT GGTCTAATTT GTAGATATCC TCAAGAAGAT 
TACGAATCTT TCTCACTCCC AGAATCTGTT CCCCTATTTT GTTTACCAAT GGGTGCAACT 
ATTGAATGTT GGCCACCAAA TAGCAAATAC CCTCTCCCTG TATTTTCTAC ATTTGTGTTA 
ACTGGAGCCT CAGCTGAAAA GGTCTATGGT GCTGCTATTC AGTTTTATGA ACCATACTCT 
GAGGAGAATC TCACAGAAAA ACAGAGACTT CTTTTGGGTT TAGCATCATC AGCAGATGGG 
AAATCTGATA GTTCTAAAAC AATTCACACT AGTAAATGCA TCTGTCTTCT TTCTCACTGG 
CCTTTTTTTG ATGCATTCAG GAAATTTTTG ACCTTTCTGT ATCGTTACTC TATCTCTGGA 
CCTCATGTCC TTCCAATTGA GAAGCATATT TCTCATTTTA TGCATAAAGT TCCTTTTCCA 
TCTCCTCAGA GGCCACGGAT TTTAGTTCAG TTATCGCCAC ATGATAATCT GATTCTCAGT 
CAACCTGTTT CTTCACCTCT TCCACTAAGT GGTGGCAAAT TTTCAACACT ACTTCAAAAT 
TTAGGCCCTG AAAATGCTGT AACACTACTG GTGTTTGCAG TAACAGAGCA CAAGATTCTC 
ATCCATTCCT TACGGCCTTC CGTGCTTACT AGCGTGACAG AAGCATTAGT GTCTATGATT 
TTCCCTTTCC ACTGGCCGTG CCCATATGTT CCTCTCTGCC CACTGGCTTT AGCAGATGTC 
TTGAGTGCAC CATGTCCATT CATTGTAGGA ATTGATTCAA GATATTTTGA TCTCTATGAC 
CCTCCACCAG ATGTTAGTTG TGTTGACCTA GATACCAACA CTATTTCTCA AACTGGCGAT 
AAGAAAAATG TAGCCTGGAA GATACTTCCA AAGAAACCAT GTAAAAACCT GATGAACACA 
CTGAATAATT TGCACCAGCA GTTGGCAAAA TTGCAGCAGA GACCAAGAGA TGATGGACTA 
ATGGACTTAG CAATGAATGA CTATGATTTT AATTCTGGAA AGAGGTTGCA TATGATAGAT 
TTGGAAATCC AAGAGGCATT TTTGTGTTTC ATGGCCTCTA TTTTAAAAGG TTATAGATCA 
TACTTAAGAC CAATAACACA AGCCCCTTCT GAGACAGCCA CCGATGCATC CTCTCTTTTT 
GCCCTACAAG CTTTCTTAAG AAGCCGAGAC CGGTCACATC AAAAATTCTA TAACATGATG 
ACCAAAACAC AAATGTTCAT TCGCTTCATT GAGGAATGTT CTTTTGTCAG TGATAAAGAT 
GCAAGCCTGG CATTTTTTGA TGATTGTGTA GATAAAGTGG ATATGGACAA GAGTGGTGAA 
ATGCGACTTA TAGAACTGGA TGAATCTTTC AAAAGTGAAC ATACTGTTTT TGTAACACCA 
CCTGAGATAC CCCATCTACC CAATGGTGAA GAACCCCCTT TACAGTACAG CTATAATGGA 
TTTCCAGTTC TTAGAAACAA TTTATTTGAG AGACCTGAAG GATTTCTACA AACACGAAAG 
AACAAATTGC CTTCAAAATC AAGTAGTCCT AGTAGCCCTT CTCCCATGTT CAGAAGAACA 
AAACAGGAAA TTAAATCAGC CCATAAAATT GCCAAGAGGT ACTCTTCCAT TCCTCAGATG 
TGGTCTAGGT GTCTGCTGCG CCACTGCTAT GGACTGTGGT TCATTTGTCT GCCAGCTTAT 
GTGAAAGTGT GTCATTCAAA AGTCAGGGCA CTGAAGACAG CATATGATGT GCTCAAAAAA 
ATGCAGTCGA AAAAGATGGA TCCACCTGAT GAAGTGTGCT ACCGCATTCT CATGCAGCTC 
TGTGGGCAGT ACGATCAGCC CGTGCTTGCA GTTCGAGTGC TTTTTGAAAT GCAGAAAGCT 
GGTATTGATC CCAATGCCAT TACTTATGGT TATTATAATA AGGCTGTTTT GGAAAGCACC 
TGGCCCTCAA GAAGTCGTAG TGGCTATTTT CTTTGGACAA AAGTAAGAAA CGTTGTTTTA 
GGAGTAGCAC AGTTCAAAAG AGCTTTAAAG AAACAGGCAC ACTTATCACA AACAACTCTC 
TCAGGTGGCC AGTCTGATCT TGGATATAAT TCATTATCTA AGGATGAAGT TAGAAGAGGG 
GATATATCTA CTGAGGAAAT TCAAGAAGAA AAAGATAAGA AAGGAAGTGA TTCTAGTTCC 
CTGTCAGAGA GTGAGAGTAC AAAAGGAAGT GCTGACTGTC TTCCTAAACT CAATTATCAA 
AGTTCTTCCA GTATAGTTCG CCTCAGTGGT ACAAATAACA ATGGTGCTGA TAAAATATCA 
GGAGAAAGCA CAGAAAGCAC ATCTAAGCTG CTCTTACTAT CATCTTTTGA GGGTACAAAT 
GAAACAGGAA ATAGTCAAAA TAGATGCTTC AGGAAAAGAC ATAAAAGTGA CAACGAACCT 
AATTTGCAAC AGCGACTCTC CTGGGGAAGC AGAAACCGCA ATCTTAGTGG AGGAGTGCTG 
ATGGGACTTA TGCTTAATAG AATCAACCAG GAAGCAAACC CTGGAGACAT GGTTGAAAAA 
CTAGGAGCTG ATGCAAAAAT TCTTTCAAAC GTTATATCAC AAAGCACAAG ACCAACTAGT 
CTTGATATTG GAAAACCCCC TTTAAGATCA AAAAGAGACA GTCTAGAAAA GGAATCTAGC 
GACGATGATA CACCTTTCGA TGGTTCTAAT TGTTTGGCAG ATAAAGTGGA TTCTCCTGTT 
ATTTTTGATT TGGAAGACTT AGACAGTGAG ACAGATGGAT CAAAAGTGGG ATACATTGCT 
TCACAAAATC CCAGAAGGAT TCAGCGTATG AGCAGCAGCT TCTCGGTAAA ACCTTCTGAA 
AAAACGGATG TTGCCACAGG ATTTGATCCC CTCTCTCTTT TGGTTGCTGA GACTGAACAG 
CAGCAAAAAG AGGAAGAGGA GGAGGATGAT GAAGATAGCA AAAGCGTTTC AACACCATCC 
GCCAGGCGTG ACTTAGCCGA AGAGATTGTG ATGTATATGA ATAACATGAG CAGTCCTTTG 
ACAAGCCGCA CACCAAGCAT CGATTTACAA CGGGCATGTG ATGACAAATC AACCAGTAAG 
AAAAGTCCAA CATTTGTCAA GGCATGCAGG AGATCTAGCC TGCCTCCTCA TTCTCCGAGG 
CCAGTGAGAC TTACCAAATC TAAAAGTTAT ACAAAAACTG AGGAGAGACC TCGGGACAGA 
CTCTGGTCTT CTCCAGCCTT CTCACCTACT TGCCCATTTA GGGAAGAATC TCAAGACGCA 
TTAACCCATT CATCACCTTC ATTTAATTTA GATACACTGC TAGTACCTAA ACTAGATGTT 
CTAAGGAACA GTATGTTCAC TGCTGGGAAA GGAGTTGCCG AGAAAGCCAG CAAATGGTAT 
TCAAGATTCA CCATGTATAC CACATCATCT AAGGATCAAA GTTCTGATCG TACCAGTCTT 
TCTTCAGTGG GAGCCCAGGA TTCTGAATCT ACCTCTTTAA CAGATGAAGA TGTTTGCCAT 
GAATTGGAAG GAGCCACTTC CTCCCAAGAG AGCTGTGCTG CTTCAGGGAA TAAGGGAATT 
GATTTCAGCA GAACAAGCCT GGAAAGTTCT GCCTCCTTAG AAGGATCCCT GTCAAAGTTT 
GCCTTACCTG GGAAATCAGA AGTGGCATCT TCCTGCAACA CAAGTAATAC AAATATCTTC 
CAGAACTATG CAATGGAGGT TCTTATCTCA AGTTGCTCTC GGTGTAGAAC TTGTGATTGT 
CTTGTCCATG ATGAGGAAAT CATGGCTGGC TGGACAGCAG ATGATTCAAA TCTCAATACT 
ACATGCCCGT TTTGTGGCAA TCTCTTCTTG CCCTTTCTGA ATATTGAAAT AAGAGATTTA 
AGACGACCCG GAAGATACTT TCTGAAGTCA AGTCCATCTA CAGAAAATAT GCATTTTCCA 
TCCTCCCTTT CAAGTCAGAC AAGGCAGTCT TGTATCTCAA CATCAGCCTC TGGTCTTGAC 
ACGTCTGTTA TCTCTGTTCA AGGGAATTTT GATCTCAATA GCAAATCTAA ACTGCTGGAA 
AATACATGTG CCCGAACTAT TCACATCCCT GCTGATAGAT CAAAAACAGG TGTGTCAAAA 
TGTCCAATAT TTCCAATGGC CAGGAGTGTT AGTACTTATG GCCCCTTGGA TAAGGAAGAC 
ACTGGAGGCC AGAAGCTCAT TCCTACAGGC AGCCTGCCAA CTACTTTACA AGGAGCTACA 
GATTCTTTAG GATTAGAGTG GCACCTTCCT AGTCCAGATC CTATCACAGT TCCATATCTT 
AGTCCTTTAG TGGTATGGAA GGAGCTTGAA AGCTTACTGG AAAATGAAGG CGACCATGCA 
ATAACAGTGG CAGACTTTGT GGATCATCAT CCAATCGTGT TTTGGAACCT GGTGTGGTAT 
TTTAGACGTC TCGACTTACC CAGTAACTTA CCTGGATTGA TTCTTTCTTC TGAGCACTGT 
AACAAGTATT CAAAGATTCC TCGCCACTGT ATGTCGGAAG ATAGTAAATA CGTTTTAATA 
CAAATGTTAT GGGACAATAT GAAATTACAT CAGGATCCGG GACAGCCTTT GTACATCCTC 
TGGAATGCCC ACAGTCAAAA TCGTACTCTT TTGTTTGAGA CCCAAAAGTA TCCAATGGTC 
CATTTATTGC AAAAGGGTGA TAATTCATTT AACCAGGAAC TGTTGAAAAA TATGGTAAAA 
AGCATTAAAA TGAATGATGT CTATGGACCA ATGAGTCAAA TTTTAGAGAC ACTGAATAAA 
TGTCCTCATT TTAAAAGACA GAGGAGTTTA TATAGAGAAA TACTATTTCT CTCACTTGTG 
GCACTGGGAA GAGAAAACAT TGATATAGAT GCATTTGATA AAGAATACAA GATGGCATAC 
GATCGTCTCA CACCTAGTCA AGTCAAGAGT ACACACAACT GTGATAGACC ACCAAGTACA 
GGGGTGATGG AATGTCGAAA AACCTTTGGA GAACCTTATC TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DENND4A ( XM_005638530.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005638587.1 Canis lupus familiaris DENN domain containing 4A (DENND4A), transcript variant X7, mRNA.

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CloneID	OCe29022
Clone ID Related Accession (Same CDS sequence)	XM_005638530.2 , XM_005638530.3
Accession Version	XM_005638530.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5625bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	C-myc promoter-binding protein isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006612.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005638530.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTGAAG ATAAGGGTCC TCGTGTTGCT GACTACTTTG TTGTAGCAGG ATTAACTGAT 
GTTTCAAAGC CTCTTGAAGA AGAAATTCAC TTCAATGATG CCTGTCATAA AGTAGCTAAA 
CCAAAAGAAC CTATCACAGA TGTTTCGGTG ATTATTAAAT CTCTTGGGGA AGAAGTGCCA 
CGGGATTATA TATGTATTGA TGTTACCCCA ACTGGATTGT CGGCTGATCT CAATAATGGA 
AGTCTTGTGG GGCCACAGAT TTACCTTTGC TACAGAAGAG GAAGAGATAA GCCTCCACTT 
ACAGATCTGG GGGTTTTGTA TGACTGGAAA GAAAGATTGA AACAGGGATG TGAAATTATT 
CAGAGTACTC CCTATGGACA CCCTGCAAAT ATTAGTGGCA GTACCTCATC ACAAAAAATT 
TATATAACTT ACCGAAGAGC CTCTGAAAAC ATGACGCAAA ATACGTTGGC TGTCACAGAC 
ATATGTATTA TTATACCCAG TAAAGGAGAG AGTCCACCAC ACACGTTTTG CAAAGTCGAT 
AAGAATCTCA ATAACAGTAT GTGGGGATCT GCAGTGTACT TATGTTATAA AAAATCAGTG 
GCAAAGACTA ACACTATATC CTATAAAGCT GGTCTAATTT GTAGATATCC TCAAGAAGAT 
TACGAATCTT TCTCACTCCC AGAATCTGTT CCCCTATTTT GTTTACCAAT GGGTGCAACT 
ATTGAATGTT GGCCACCAAA TAGCAAATAC CCTCTCCCTG TATTTTCTAC ATTTGTGTTA 
ACTGGAGCCT CAGCTGAAAA GGTCTATGGT GCTGCTATTC AGTTTTATGA ACCATACTCT 
GAGGAGAATC TCACAGAAAA ACAGAGACTT CTTTTGGGTT TAGCATCATC AGCAGATGGG 
AAATCTGATA GTTCTAAAAC AATTCACACT AGTAAATGCA TCTGTCTTCT TTCTCACTGG 
CCTTTTTTTG ATGCATTCAG GAAATTTTTG ACCTTTCTGT ATCGTTACTC TATCTCTGGA 
CCTCATGTCC TTCCAATTGA GAAGCATATT TCTCATTTTA TGCATAAAGT TCCTTTTCCA 
TCTCCTCAGA GGCCACGGAT TTTAGTTCAG TTATCGCCAC ATGATAATCT GATTCTCAGT 
CAACCTGTTT CTTCACCTCT TCCACTAAGT GGTGGCAAAT TTTCAACACT ACTTCAAAAT 
TTAGGCCCTG AAAATGCTGT AACACTACTG GTGTTTGCAG TAACAGAGCA CAAGATTCTC 
ATCCATTCCT TACGGCCTTC CGTGCTTACT AGCGTGACAG AAGCATTAGT GTCTATGATT 
TTCCCTTTCC ACTGGCCGTG CCCATATGTT CCTCTCTGCC CACTGGCTTT AGCAGATGTC 
TTGAGTGCAC CATGTCCATT CATTGTAGGA ATTGATTCAA GATATTTTGA TCTCTATGAC 
CCTCCACCAG ATGTTAGTTG TGTTGACCTA GATACCAACA CTATTTCTCA AACTGGCGAT 
AAGAAAAATG TAGCCTGGAA GATACTTCCA AAGAAACCAT GTAAAAACCT GATGAACACA 
CTGAATAATT TGCACCAGCA GTTGGCAAAA TTGCAGCAGA GACCAAGAGA TGATGGACTA 
ATGGACTTAG CAATGAATGA CTATGATTTT AATTCTGGAA AGAGGTTGCA TATGATAGAT 
TTGGAAATCC AAGAGGCATT TTTGTGTTTC ATGGCCTCTA TTTTAAAAGG TTATAGATCA 
TACTTAAGAC CAATAACACA AGCCCCTTCT GAGACAGCCA CCGATGCATC CTCTCTTTTT 
GCCCTACAAG CTTTCTTAAG AAGCCGAGAC CGGTCACATC AAAAATTCTA TAACATGATG 
ACCAAAACAC AAATGTTCAT TCGCTTCATT GAGGAATGTT CTTTTGTCAG TGATAAAGAT 
GCAAGCCTGG CATTTTTTGA TGATTGTGTA GATAAAGTGG ATATGGACAA GAGTGGTGAA 
ATGCGACTTA TAGAACTGGA TGAATCTTTC AAAAGTGAAC ATACTGTTTT TGTAACACCA 
CCTGAGATAC CCCATCTACC CAATGGTGAA GAACCCCCTT TACAGTACAG CTATAATGGA 
TTTCCAGTTC TTAGAAACAA TTTATTTGAG AGACCTGAAG GATTTCTACA AACACGAAAG 
AACAAATTGC CTTCAAAATC AAGTAGTCCT AGTAGCCCTT CTCCCATGTT CAGAAGAACA 
AAACAGGAAA TTAAATCAGC CCATAAAATT GCCAAGAGGT ACTCTTCCAT TCCTCAGATG 
TGGTCTAGGT GTCTGCTGCG CCACTGCTAT GGACTGTGGT TCATTTGTCT GCCAGCTTAT 
GTGAAAGTGT GTCATTCAAA AGTCAGGGCA CTGAAGACAG CATATGATGT GCTCAAAAAA 
ATGCAGTCGA AAAAGATGGA TCCACCTGAT GAAGTGTGCT ACCGCATTCT CATGCAGCTC 
TGTGGGCAGT ACGATCAGCC CGTGCTTGCA GTTCGAGTGC TTTTTGAAAT GCAGAAAGCT 
GGTATTGATC CCAATGCCAT TACTTATGGT TATTATAATA AGGCTGTTTT GGAAAGCACC 
TGGCCCTCAA GAAGTCGTAG TGGCTATTTT CTTTGGACAA AAGTAAGAAA CGTTGTTTTA 
GGAGTAGCAC AGTTCAAAAG AGCTTTAAAG AAACAGGCAC ACTTATCACA AACAACTCTC 
TCAGGTGGCC AGTCTGATCT TGGATATAAT TCATTATCTA AGGATGAAGT TAGAAGAGGG 
GATATATCTA CTGAGGAAAT TCAAGAAGAA AAAGATAAGA AAGGAAGTGA TTCTAGTTCC 
CTGTCAGAGA GTGAGAGTAC AAAAGGAAGT GCTGACTGTC TTCCTAAACT CAATTATCAA 
AGTTCTTCCA GTATAGTTCG CCTCAGTGGT ACAAATAACA ATGGTGCTGA TAAAATATCA 
GGAGAAAGCA CAGAAAGCAC ATCTAAGCTG CTCTTACTAT CATCTTTTGA GGGTACAAAT 
GAAACAGGAA ATAGTCAAAA TAGATGCTTC AGGAAAAGAC ATAAAAGTGA CAACGAACCT 
AATTTGCAAC AGCGACTCTC CTGGGGAAGC AGAAACCGCA ATCTTAGTGG AGGAGTGCTG 
ATGGGACTTA TGCTTAATAG AATCAACCAG GAAGCAAACC CTGGAGACAT GGTTGAAAAA 
CTAGGAGCTG ATGCAAAAAT TCTTTCAAAC GTTATATCAC AAAGCACAAG ACCAACTAGT 
CTTGATATTG GAAAACCCCC TTTAAGATCA AAAAGAGACA GTCTAGAAAA GGAATCTAGC 
GACGATGATA CACCTTTCGA TGGTTCTAAT TGTTTGGCAG ATAAAGTGGA TTCTCCTGTT 
ATTTTTGATT TGGAAGACTT AGACAGTGAG ACAGATGGAT CAAAAGTGGG ATACATTGCT 
TCACAAAATC CCAGAAGGAT TCAGCGTATG AGCAGCAGCT TCTCGGTAAA ACCTTCTGAA 
AAAACGGATG TTGCCACAGG ATTTGATCCC CTCTCTCTTT TGGTTGCTGA GACTGAACAG 
CAGCAAAAAG AGGAAGAGGA GGAGGATGAT GAAGATAGCA AAAGCGTTTC AACACCATCC 
GCCAGGCGTG ACTTAGCCGA AGAGATTGTG ATGTATATGA ATAACATGAG CAGTCCTTTG 
ACAAGCCGCA CACCAAGCAT CGATTTACAA CGGGCATGTG ATGACAAATC AACCAGTAAG 
AAAAGTCCAA CATTTGTCAA GGCATGCAGG AGATCTAGCC TGCCTCCTCA TTCTCCGAGG 
CCAGTGAGAC TTACCAAATC TAAAAGTTAT ACAAAAACTG AGGAGAGACC TCGGGACAGA 
CTCTGGTCTT CTCCAGCCTT CTCACCTACT TGCCCATTTA GGGAAGAATC TCAAGACGCA 
TTAACCCATT CATCACCTTC ATTTAATTTA GATACACTGC TAGTACCTAA ACTAGATGTT 
CTAAGGAACA GTATGTTCAC TGCTGGGAAA GGAGTTGCCG AGAAAGCCAG CAAATGGTAT 
TCAAGATTCA CCATGTATAC CACATCATCT AAGGATCAAA GTTCTGATCG TACCAGTCTT 
TCTTCAGTGG GAGCCCAGGA TTCTGAATCT ACCTCTTTAA CAGATGAAGA TGTTTGCCAT 
GAATTGGAAG GAGCCACTTC CTCCCAAGAG AGCTGTGCTG CTTCAGGGAA TAAGGGAATT 
GATTTCAGCA GAACAAGCCT GGAAAGTTCT GCCTCCTTAG AAGGATCCCT GTCAAAGTTT 
GCCTTACCTG GGAAATCAGA AGTGGCATCT TCCTGCAACA CAAGTAATAC AAATATCTTC 
CAGAACTATG CAATGGAGGT TCTTATCTCA AGTTGCTCTC GGTGTAGAAC TTGTGATTGT 
CTTGTCCATG ATGAGGAAAT CATGGCTGGC TGGACAGCAG ATGATTCAAA TCTCAATACT 
ACATGCCCGT TTTGTGGCAA TCTCTTCTTG CCCTTTCTGA ATATTGAAAT AAGAGATTTA 
AGACGACCCG GAAGATACTT TCTGAAGTCA AGTCCATCTA CAGAAAATAT GCATTTTCCA 
TCCTCCCTTT CAAGTCAGAC AAGGCAGTCT TGTATCTCAA CATCAGCCTC TGGTCTTGAC 
ACGTCTGTTA TCTCTGTTCA AGGGAATTTT GATCTCAATA GCAAATCTAA ACTGCTGGAA 
AATACATGTG CCCGAACTAT TCACATCCCT GCTGATAGAT CAAAAACAGG TGTGTCAAAA 
TGTCCAATAT TTCCAATGGC CAGGAGTGTT AGTACTTATG GCCCCTTGGA TAAGGAAGAC 
ACTGGAGGCC AGAAGCTCAT TCCTACAGGC AGCCTGCCAA CTACTTTACA AGGAGCTACA 
GATTCTTTAG GATTAGAGTG GCACCTTCCT AGTCCAGATC CTATCACAGT TCCATATCTT 
AGTCCTTTAG TGGTATGGAA GGAGCTTGAA AGCTTACTGG AAAATGAAGG CGACCATGCA 
ATAACAGTGG CAGACTTTGT GGATCATCAT CCAATCGTGT TTTGGAACCT GGTGTGGTAT 
TTTAGACGTC TCGACTTACC CAGTAACTTA CCTGGATTGA TTCTTTCTTC TGAGCACTGT 
AACAAGTATT CAAAGATTCC TCGCCACTGT ATGTCGGAAG ATAGTAAATA CGTTTTAATA 
CAAATGTTAT GGGACAATAT GAAATTACAT CAGGATCCGG GACAGCCTTT GTACATCCTC 
TGGAATGCCC ACAGTCAAAA TCGTACTCTT TTGTTTGAGA CCCAAAAGTA TCCAATGGTC 
CATTTATTGC AAAAGGGTGA TAATTCATTT AACCAGGAAC TGTTGAAAAA TATGGTAAAA 
AGCATTAAAA TGAATGATGT CTATGGACCA ATGAGTCAAA TTTTAGAGAC ACTGAATAAA 
TGTCCTCATT TTAAAAGACA GAGGAGTTTA TATAGAGAAA TACTATTTCT CTCACTTGTG 
GCACTGGGAA GAGAAAACAT TGATATAGAT GCATTTGATA AAGAATACAA GATGGCATAC 
GATCGTCTCA CACCTAGTCA AGTCAAGAGT ACACACAACT GTGATAGACC ACCAAGTACA 
GGGGTGATGG AATGTCGAAA AACCTTTGGA GAACCTTATC TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005638587.1
CDS	344..5968
Translation	MIEDKGPRVADYFVVAGLTDVSKPLEEEIHFNDACHKVAKPKEPITDVSVIIKSLGEEVPRDYICIDVTPTGLSADLNNGSLVGPQIYLCYRRGRDKPPLTDLGVLYDWKERLKQGCEIIQSTPYGHPANISGSTSSQKIYITYRRASENMTQNTLAVTDICIIIPSKGESPPHTFCKVDKNLNNSMWGSAVYLCYKKSVAKTNTISYKAGLICRYPQEDYESFSLPESVPLFCLPMGATIECWPPNSKYPLPVFSTFVLTGASAEKVYGAAIQFYEPYSEENLTEKQRLLLGLASSADGKSDSSKTIHTSKCICLLSHWPFFDAFRKFLTFLYRYSISGPHVLPIEKHISHFMHKVPFPSPQRPRILVQLSPHDNLILSQPVSSPLPLSGGKFSTLLQNLGPENAVTLLVFAVTEHKILIHSLRPSVLTSVTEALVSMIFPFHWPCPYVPLCPLALADVLSAPCPFIVGIDSRYFDLYDPPPDVSCVDLDTNTISQTGDKKNVAWKILPKKPCKNLMNTLNNLHQQLAKLQQRPRDDGLMDLAMNDYDFNSGKRLHMIDLEIQEAFLCFMASILKGYRSYLRPITQAPSETATDASSLFALQAFLRSRDRSHQKFYNMMTKTQMFIRFIEECSFVSDKDASLAFFDDCVDKVDMDKSGEMRLIELDESFKSEHTVFVTPPEIPHLPNGEEPPLQYSYNGFPVLRNNLFERPEGFLQTRKNKLPSKSSSPSSPSPMFRRTKQEIKSAHKIAKRYSSIPQMWSRCLLRHCYGLWFICLPAYVKVCHSKVRALKTAYDVLKKMQSKKMDPPDEVCYRILMQLCGQYDQPVLAVRVLFEMQKAGIDPNAITYGYYNKAVLESTWPSRSRSGYFLWTKVRNVVLGVAQFKRALKKQAHLSQTTLSGGQSDLGYNSLSKDEVRRGDISTEEIQEEKDKKGSDSSSLSESESTKGSADCLPKLNYQSSSSIVRLSGTNNNGADKISGESTESTSKLLLLSSFEGTNETGNSQNRCFRKRHKSDNEPNLQQRLSWGSRNRNLSGGVLMGLMLNRINQEANPGDMVEKLGADAKILSNVISQSTRPTSLDIGKPPLRSKRDSLEKESSDDDTPFDGSNCLADKVDSPVIFDLEDLDSETDGSKVGYIASQNPRRIQRMSSSFSVKPSEKTDVATGFDPLSLLVAETEQQQKEEEEEDDEDSKSVSTPSARRDLAEEIVMYMNNMSSPLTSRTPSIDLQRACDDKSTSKKSPTFVKACRRSSLPPHSPRPVRLTKSKSYTKTEERPRDRLWSSPAFSPTCPFREESQDALTHSSPSFNLDTLLVPKLDVLRNSMFTAGKGVAEKASKWYSRFTMYTTSSKDQSSDRTSLSSVGAQDSESTSLTDEDVCHELEGATSSQESCAASGNKGIDFSRTSLESSASLEGSLSKFALPGKSEVASSCNTSNTNIFQNYAMEVLISSCSRCRTCDCLVHDEEIMAGWTADDSNLNTTCPFCGNLFLPFLNIEIRDLRRPGRYFLKSSPSTENMHFPSSLSSQTRQSCISTSASGLDTSVISVQGNFDLNSKSKLLENTCARTIHIPADRSKTGVSKCPIFPMARSVSTYGPLDKEDTGGQKLIPTGSLPTTLQGATDSLGLEWHLPSPDPITVPYLSPLVVWKELESLLENEGDHAITVADFVDHHPIVFWNLVWYFRRLDLPSNLPGLILSSEHCNKYSKIPRHCMSEDSKYVLIQMLWDNMKLHQDPGQPLYILWNAHSQNRTLLFETQKYPMVHLLQKGDNSFNQELLKNMVKSIKMNDVYGPMSQILETLNKCPHFKRQRSLYREILFLSLVALGRENIDIDAFDKEYKMAYDRLTPSQVKSTHNCDRPPSTGVMECRKTFGEPYL

Target ORF information:

RefSeq Version	XM_005638530.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris DENN domain containing 4A (DENND4A), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005638530.3

ORF Insert Sequence:

ATGATTGAAG ATAAGGGTCC TCGTGTTGCT GACTACTTTG TTGTAGCAGG ATTAACTGAT 
GTTTCAAAGC CTCTTGAAGA AGAAATTCAC TTCAATGATG CCTGTCATAA AGTAGCTAAA 
CCAAAAGAAC CTATCACAGA TGTTTCGGTG ATTATTAAAT CTCTTGGGGA AGAAGTGCCA 
CGGGATTATA TATGTATTGA TGTTACCCCA ACTGGATTGT CGGCTGATCT CAATAATGGA 
AGTCTTGTGG GGCCACAGAT TTACCTTTGC TACAGAAGAG GAAGAGATAA GCCTCCACTT 
ACAGATCTGG GGGTTTTGTA TGACTGGAAA GAAAGATTGA AACAGGGATG TGAAATTATT 
CAGAGTACTC CCTATGGACA CCCTGCAAAT ATTAGTGGCA GTACCTCATC ACAAAAAATT 
TATATAACTT ACCGAAGAGC CTCTGAAAAC ATGACGCAAA ATACGTTGGC TGTCACAGAC 
ATATGTATTA TTATACCCAG TAAAGGAGAG AGTCCACCAC ACACGTTTTG CAAAGTCGAT 
AAGAATCTCA ATAACAGTAT GTGGGGATCT GCAGTGTACT TATGTTATAA AAAATCAGTG 
GCAAAGACTA ACACTATATC CTATAAAGCT GGTCTAATTT GTAGATATCC TCAAGAAGAT 
TACGAATCTT TCTCACTCCC AGAATCTGTT CCCCTATTTT GTTTACCAAT GGGTGCAACT 
ATTGAATGTT GGCCACCAAA TAGCAAATAC CCTCTCCCTG TATTTTCTAC ATTTGTGTTA 
ACTGGAGCCT CAGCTGAAAA GGTCTATGGT GCTGCTATTC AGTTTTATGA ACCATACTCT 
GAGGAGAATC TCACAGAAAA ACAGAGACTT CTTTTGGGTT TAGCATCATC AGCAGATGGG 
AAATCTGATA GTTCTAAAAC AATTCACACT AGTAAATGCA TCTGTCTTCT TTCTCACTGG 
CCTTTTTTTG ATGCATTCAG GAAATTTTTG ACCTTTCTGT ATCGTTACTC TATCTCTGGA 
CCTCATGTCC TTCCAATTGA GAAGCATATT TCTCATTTTA TGCATAAAGT TCCTTTTCCA 
TCTCCTCAGA GGCCACGGAT TTTAGTTCAG TTATCGCCAC ATGATAATCT GATTCTCAGT 
CAACCTGTTT CTTCACCTCT TCCACTAAGT GGTGGCAAAT TTTCAACACT ACTTCAAAAT 
TTAGGCCCTG AAAATGCTGT AACACTACTG GTGTTTGCAG TAACAGAGCA CAAGATTCTC 
ATCCATTCCT TACGGCCTTC CGTGCTTACT AGCGTGACAG AAGCATTAGT GTCTATGATT 
TTCCCTTTCC ACTGGCCGTG CCCATATGTT CCTCTCTGCC CACTGGCTTT AGCAGATGTC 
TTGAGTGCAC CATGTCCATT CATTGTAGGA ATTGATTCAA GATATTTTGA TCTCTATGAC 
CCTCCACCAG ATGTTAGTTG TGTTGACCTA GATACCAACA CTATTTCTCA AACTGGCGAT 
AAGAAAAATG TAGCCTGGAA GATACTTCCA AAGAAACCAT GTAAAAACCT GATGAACACA 
CTGAATAATT TGCACCAGCA GTTGGCAAAA TTGCAGCAGA GACCAAGAGA TGATGGACTA 
ATGGACTTAG CAATGAATGA CTATGATTTT AATTCTGGAA AGAGGTTGCA TATGATAGAT 
TTGGAAATCC AAGAGGCATT TTTGTGTTTC ATGGCCTCTA TTTTAAAAGG TTATAGATCA 
TACTTAAGAC CAATAACACA AGCCCCTTCT GAGACAGCCA CCGATGCATC CTCTCTTTTT 
GCCCTACAAG CTTTCTTAAG AAGCCGAGAC CGGTCACATC AAAAATTCTA TAACATGATG 
ACCAAAACAC AAATGTTCAT TCGCTTCATT GAGGAATGTT CTTTTGTCAG TGATAAAGAT 
GCAAGCCTGG CATTTTTTGA TGATTGTGTA GATAAAGTGG ATATGGACAA GAGTGGTGAA 
ATGCGACTTA TAGAACTGGA TGAATCTTTC AAAAGTGAAC ATACTGTTTT TGTAACACCA 
CCTGAGATAC CCCATCTACC CAATGGTGAA GAACCCCCTT TACAGTACAG CTATAATGGA 
TTTCCAGTTC TTAGAAACAA TTTATTTGAG AGACCTGAAG GATTTCTACA AACACGAAAG 
AACAAATTGC CTTCAAAATC AAGTAGTCCT AGTAGCCCTT CTCCCATGTT CAGAAGAACA 
AAACAGGAAA TTAAATCAGC CCATAAAATT GCCAAGAGGT ACTCTTCCAT TCCTCAGATG 
TGGTCTAGGT GTCTGCTGCG CCACTGCTAT GGACTGTGGT TCATTTGTCT GCCAGCTTAT 
GTGAAAGTGT GTCATTCAAA AGTCAGGGCA CTGAAGACAG CATATGATGT GCTCAAAAAA 
ATGCAGTCGA AAAAGATGGA TCCACCTGAT GAAGTGTGCT ACCGCATTCT CATGCAGCTC 
TGTGGGCAGT ACGATCAGCC CGTGCTTGCA GTTCGAGTGC TTTTTGAAAT GCAGAAAGCT 
GGTATTGATC CCAATGCCAT TACTTATGGT TATTATAATA AGGCTGTTTT GGAAAGCACC 
TGGCCCTCAA GAAGTCGTAG TGGCTATTTT CTTTGGACAA AAGTAAGAAA CGTTGTTTTA 
GGAGTAGCAC AGTTCAAAAG AGCTTTAAAG AAACAGGCAC ACTTATCACA AACAACTCTC 
TCAGGTGGCC AGTCTGATCT TGGATATAAT TCATTATCTA AGGATGAAGT TAGAAGAGGG 
GATATATCTA CTGAGGAAAT TCAAGAAGAA AAAGATAAGA AAGGAAGTGA TTCTAGTTCC 
CTGTCAGAGA GTGAGAGTAC AAAAGGAAGT GCTGACTGTC TTCCTAAACT CAATTATCAA 
AGTTCTTCCA GTATAGTTCG CCTCAGTGGT ACAAATAACA ATGGTGCTGA TAAAATATCA 
GGAGAAAGCA CAGAAAGCAC ATCTAAGCTG CTCTTACTAT CATCTTTTGA GGGTACAAAT 
GAAACAGGAA ATAGTCAAAA TAGATGCTTC AGGAAAAGAC ATAAAAGTGA CAACGAACCT 
AATTTGCAAC AGCGACTCTC CTGGGGAAGC AGAAACCGCA ATCTTAGTGG AGGAGTGCTG 
ATGGGACTTA TGCTTAATAG AATCAACCAG GAAGCAAACC CTGGAGACAT GGTTGAAAAA 
CTAGGAGCTG ATGCAAAAAT TCTTTCAAAC GTTATATCAC AAAGCACAAG ACCAACTAGT 
CTTGATATTG GAAAACCCCC TTTAAGATCA AAAAGAGACA GTCTAGAAAA GGAATCTAGC 
GACGATGATA CACCTTTCGA TGGTTCTAAT TGTTTGGCAG ATAAAGTGGA TTCTCCTGTT 
ATTTTTGATT TGGAAGACTT AGACAGTGAG ACAGATGGAT CAAAAGTGGG ATACATTGCT 
TCACAAAATC CCAGAAGGAT TCAGCGTATG AGCAGCAGCT TCTCGGTAAA ACCTTCTGAA 
AAAACGGATG TTGCCACAGG ATTTGATCCC CTCTCTCTTT TGGTTGCTGA GACTGAACAG 
CAGCAAAAAG AGGAAGAGGA GGAGGATGAT GAAGATAGCA AAAGCGTTTC AACACCATCC 
GCCAGGCGTG ACTTAGCCGA AGAGATTGTG ATGTATATGA ATAACATGAG CAGTCCTTTG 
ACAAGCCGCA CACCAAGCAT CGATTTACAA CGGGCATGTG ATGACAAATC AACCAGTAAG 
AAAAGTCCAA CATTTGTCAA GGCATGCAGG AGATCTAGCC TGCCTCCTCA TTCTCCGAGG 
CCAGTGAGAC TTACCAAATC TAAAAGTTAT ACAAAAACTG AGGAGAGACC TCGGGACAGA 
CTCTGGTCTT CTCCAGCCTT CTCACCTACT TGCCCATTTA GGGAAGAATC TCAAGACGCA 
TTAACCCATT CATCACCTTC ATTTAATTTA GATACACTGC TAGTACCTAA ACTAGATGTT 
CTAAGGAACA GTATGTTCAC TGCTGGGAAA GGAGTTGCCG AGAAAGCCAG CAAATGGTAT 
TCAAGATTCA CCATGTATAC CACATCATCT AAGGATCAAA GTTCTGATCG TACCAGTCTT 
TCTTCAGTGG GAGCCCAGGA TTCTGAATCT ACCTCTTTAA CAGATGAAGA TGTTTGCCAT 
GAATTGGAAG GAGCCACTTC CTCCCAAGAG AGCTGTGCTG CTTCAGGGAA TAAGGGAATT 
GATTTCAGCA GAACAAGCCT GGAAAGTTCT GCCTCCTTAG AAGGATCCCT GTCAAAGTTT 
GCCTTACCTG GGAAATCAGA AGTGGCATCT TCCTGCAACA CAAGTAATAC AAATATCTTC 
CAGAACTATG CAATGGAGGT TCTTATCTCA AGTTGCTCTC GGTGTAGAAC TTGTGATTGT 
CTTGTCCATG ATGAGGAAAT CATGGCTGGC TGGACAGCAG ATGATTCAAA TCTCAATACT 
ACATGCCCGT TTTGTGGCAA TCTCTTCTTG CCCTTTCTGA ATATTGAAAT AAGAGATTTA 
AGACGACCCG GAAGATACTT TCTGAAGTCA AGTCCATCTA CAGAAAATAT GCATTTTCCA 
TCCTCCCTTT CAAGTCAGAC AAGGCAGTCT TGTATCTCAA CATCAGCCTC TGGTCTTGAC 
ACGTCTGTTA TCTCTGTTCA AGGGAATTTT GATCTCAATA GCAAATCTAA ACTGCTGGAA 
AATACATGTG CCCGAACTAT TCACATCCCT GCTGATAGAT CAAAAACAGG TGTGTCAAAA 
TGTCCAATAT TTCCAATGGC CAGGAGTGTT AGTACTTATG GCCCCTTGGA TAAGGAAGAC 
ACTGGAGGCC AGAAGCTCAT TCCTACAGGC AGCCTGCCAA CTACTTTACA AGGAGCTACA 
GATTCTTTAG GATTAGAGTG GCACCTTCCT AGTCCAGATC CTATCACAGT TCCATATCTT 
AGTCCTTTAG TGGTATGGAA GGAGCTTGAA AGCTTACTGG AAAATGAAGG CGACCATGCA 
ATAACAGTGG CAGACTTTGT GGATCATCAT CCAATCGTGT TTTGGAACCT GGTGTGGTAT 
TTTAGACGTC TCGACTTACC CAGTAACTTA CCTGGATTGA TTCTTTCTTC TGAGCACTGT 
AACAAGTATT CAAAGATTCC TCGCCACTGT ATGTCGGAAG ATAGTAAATA CGTTTTAATA 
CAAATGTTAT GGGACAATAT GAAATTACAT CAGGATCCGG GACAGCCTTT GTACATCCTC 
TGGAATGCCC ACAGTCAAAA TCGTACTCTT TTGTTTGAGA CCCAAAAGTA TCCAATGGTC 
CATTTATTGC AAAAGGGTGA TAATTCATTT AACCAGGAAC TGTTGAAAAA TATGGTAAAA 
AGCATTAAAA TGAATGATGT CTATGGACCA ATGAGTCAAA TTTTAGAGAC ACTGAATAAA 
TGTCCTCATT TTAAAAGACA GAGGAGTTTA TATAGAGAAA TACTATTTCT CTCACTTGTG 
GCACTGGGAA GAGAAAACAT TGATATAGAT GCATTTGATA AAGAATACAA GATGGCATAC 
GATCGTCTCA CACCTAGTCA AGTCAAGAGT ACACACAACT GTGATAGACC ACCAAGTACA 
GGGGTGATGG AATGTCGAAA AACCTTTGGA GAACCTTATC TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DENND4A ( XM_544733.6 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_544733.3 Canis lupus familiaris DENN domain containing 4A (DENND4A), transcript variant X8, mRNA.

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CloneID	OCe29023
Clone ID Related Accession (Same CDS sequence)	XM_544733.6 , XM_544733.5
Accession Version	XM_544733.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5598bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	C-myc promoter-binding protein isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006612.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_544733.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTGAAG ATAAGGGTCC TCGTGTTGCT GACTACTTTG TTGTAGCAGG ATTAACTGAT 
GTTTCAAAGC CTCTTGAAGA AGAAATTCAC TTCAATGATG CCTGTCATAA AGTAGCTAAA 
CCAAAAGAAC CTATCACAGA TGTTTCGGTG ATTATTAAAT CTCTTGGGGA AGAAGTGCCA 
CGGGATTATA TATGTATTGA TGTTACCCCA ACTGGATTGT CGGCTGATCT CAATAATGGA 
AGTCTTGTGG GGCCACAGAT TTACCTTTGC TACAGAAGAG GAAGAGATAA GCCTCCACTT 
ACAGATCTGG GGGTTTTGTA TGACTGGAAA GAAAGATTGA AACAGGGATG TGAAATTATT 
CAGAGTACTC CCTATGGACA CCCTGCAAAT ATTAGTGGCA GTACCTCATC ACAAAAAATT 
TATATAACTT ACCGAAGAGC CTCTGAAAAC ATGACGCAAA ATACGTTGGC TGTCACAGAC 
ATATGTATTA TTATACCCAG TAAAGGAGAG AGTCCACCAC ACACGTTTTG CAAAGTCGAT 
AAGAATCTCA ATAACAGTAT GTGGGGATCT GCAGTGTACT TATGTTATAA AAAATCAGTG 
GCAAAGACTA ACACTATATC CTATAAAGCT GGTCTAATTT GTAGATATCC TCAAGAAGAT 
TACGAATCTT TCTCACTCCC AGAATCTGTT CCCCTATTTT GTTTACCAAT GGGTGCAACT 
ATTGAATGTT GGCCACCAAA TAGCAAATAC CCTCTCCCTG TATTTTCTAC ATTTGTGTTA 
ACTGGAGCCT CAGCTGAAAA GGTCTATGGT GCTGCTATTC AGTTTTATGA ACCATACTCT 
GAGGAGAATC TCACAGAAAA ACAGAGACTT CTTTTGGGTT TAGCATCATC AGCAGATGGG 
AAATCTGATA GTTCTAAAAC AATTCACACT AGTAAATGCA TCTGTCTTCT TTCTCACTGG 
CCTTTTTTTG ATGCATTCAG GAAATTTTTG ACCTTTCTGT ATCGTTACTC TATCTCTGGA 
CCTCATGTCC TTCCAATTGA GAAGCATATT TCTCATTTTA TGCATAAAGT TCCTTTTCCA 
TCTCCTCAGA GGCCACGGAT TTTAGTTCAG TTATCGCCAC ATGATAATCT GATTCTCAGT 
CAACCTGTTT CTTCACCTCT TCCACTAAGT GGTGGCAAAT TTTCAACACT ACTTCAAAAT 
TTAGGCCCTG AAAATGCTGT AACACTACTG GTGTTTGCAG TAACAGAGCA CAAGATTCTC 
ATCCATTCCT TACGGCCTTC CGTGCTTACT AGCGTGACAG AAGCATTAGT GTCTATGATT 
TTCCCTTTCC ACTGGCCGTG CCCATATGTT CCTCTCTGCC CACTGGCTTT AGCAGATGTC 
TTGAGTGCAC CATGTCCATT CATTGTAGGA ATTGATTCAA GATATTTTGA TCTCTATGAC 
CCTCCACCAG ATGTTAGTTG TGTTGACCTA GATACCAACA CTATTTCTCA AACTGGCGAT 
AAGAAAAATG TAGCCTGGAA GATACTTCCA AAGAAACCAT GTAAAAACCT GATGAACACA 
CTGAATAATT TGCACCAGCA GTTGGCAAAA TTGCAGCAGA GACCAAGAGA TGATGGACTA 
ATGGACTTAG CAATGAATGA CTATGATTTT AATTCTGGAA AGAGGTTGCA TATGATAGAT 
TTGGAAATCC AAGAGGCATT TTTGTGTTTC ATGGCCTCTA TTTTAAAAGG TTATAGATCA 
TACTTAAGAC CAATAACACA AGCCCCTTCT GAGACAGCCA CCGATGCATC CTCTCTTTTT 
GCCCTACAAG CTTTCTTAAG AAGCCGAGAC CGGTCACATC AAAAATTCTA TAACATGATG 
ACCAAAACAC AAATGTTCAT TCGCTTCATT GAGGAATGTT CTTTTGTCAG TGATAAAGAT 
GCAAGCCTGG CATTTTTTGA TGATTGTGTA GATAAAGTGG ATATGGACAA GAGTGGTGAA 
ATGCGACTTA TAGAACTGGA TGAATCTTTC AAAAGTGAAC ATACTGTTTT TGTAACACCA 
CCTGAGATAC CCCATCTACC CAATGGTGAA GAACCCCCTT TACAGTACAG CTATAATGGA 
TTTCCAGTTC TTAGAAACAA TTTATTTGAG AGACCTGAAG GATTTCTACA AACACGAAAG 
AACAAATTGC CTTCAAAATC AAGTAGTCCT AGTAGCCCTT CTCCCATGTT CAGAAGAACA 
AAACAGGAAA TTAAATCAGC CCATAAAATT GCCAAGAGGT ACTCTTCCAT TCCTCAGATG 
TGGTCTAGGT GTCTGCTGCG CCACTGCTAT GGACTGTGGT TCATTTGTCT GCCAGCTTAT 
GTGAAAGTGT GTCATTCAAA AGTCAGGGCA CTGAAGACAG CATATGATGT GCTCAAAAAA 
ATGCAGTCGA AAAAGATGGA TCCACCTGAT GAAGTGTGCT ACCGCATTCT CATGCAGCTC 
TGTGGGCAGT ACGATCAGCC CGTGCTTGCA GTTCGAGTGC TTTTTGAAAT GCAGAAAGCT 
GGTATTGATC CCAATGCCAT TACTTATGGT TATTATAATA AGGCTGTTTT GGAAAGCACC 
TGGCCCTCAA GAAGTCGTAG TGGCTATTTT CTTTGGACAA AAGTAAGAAA CGTTGTTTTA 
GGAGTAGCAC AGTTCAAAAG AGCTTTAAAG AAACAGGCAC ACTTATCACA AACAACTCTC 
TCAGGTGGCC AGTCTGATCT TGGATATAAT TCATTATCTA AGGATGAAGT TAGAAGAGGG 
GATATATCTA CTGAGGAAAT TCAAGAAGAA AAAGATAAGA AAGGAAGTGA TTCTAGTTCC 
CTGTCAGAGA GTGAGAGTAC AAAAGGAAGT GCTGACTGTC TTCCTAAACT CAATTATCAA 
AGTTCTTCCA GTATAGTTCG CCTCAGTGGT ACAAATAACA ATGGTGCTGA TAAAATATCA 
GGAGAAAGCA CAGAAAGCAC ATCTAAGCTG CTCTTACTAT CATCTTTTGA GGGTACAAAT 
GAAACAGGAA ATAGTCAAAA TAGATGCTTC AGGAAAAGAC ATAAAAGTGA CAACGAACCT 
AATTTGCAAC AGCGACTCTC CTGGGGAAGC AGAAACCGCA ATCTTAGTGG AGGAGTGCTG 
ATGGGACTTA TGCTTAATAG AATCAACCAG GAAGCAAACC CTGGAGACAT GGTTGAAAAA 
CTAGGAGCTG ATGCAAAAAT TCTTTCAAAC GTTATATCAC AAAGCACAAG ACCAACTAGT 
CTTGATATTG GAAAACCCCC TTTAAGATCA AAAAGAGACA GTCTAGAAAA GGAATCTAGC 
GACGATGATA CACCTTTCGA TGGTTCTAAT TGTTTGGCAG ATAAAGTGGA TTCTCCTGTT 
ATTTTTGATT TGGAAGACTT AGACAGTGAG ACAGATGGAT CAAAAGTGGG ATACATTGCT 
TCACAAAATC CCAGAAGGAT TCAGCGTATG AGCAGCAGCT TCTCGGTAAA ACCTTCTGAA 
AAAACGGATG TTGCCACAGG ATTTGATCCC CTCTCTCTTT TGGTTGCTGA GACTGAACAG 
CAGCAAAAAG AGGAAGAGGA GGAGGATGAT GAAGATAGCA AAAGCGTTTC AACACCATCC 
GCCAGGCGTG ACTTAGCCGA AGAGATTGTG ATGTATATGA ATAACATGAG CAGTCCTTTG 
ACAAGCCGCA CACCAAGCAT CGATTTACAA CGGGCATGTG ATGACAAATC AACCAGTAAG 
AAAAGTCCAA CATTTGTCAA GGCATGCAGG AGATCTAGCC TGCCTCCTCA TTCTCCGAGG 
CCAGTGAGAC TTACCAAATC TAAAAGTTAT ACAAAAACTG AGGAGAGACC TCGGGACAGA 
CTCTGGTCTT CTCCAGCCTT CTCACCTACT TGCCCATTTA GGGAAGAATC TCAAGACGCA 
TTAACCCATT CATCACCTTC ATTTAATTTA GATACACTGC TAGTACCTAA ACTAGATGTT 
CTAAGGAACA GTATGTTCAC TGCTGGGAAA GGAGTTGCCG AGAAAGCCAG CAAATGGTAT 
TCAAGATTCA CCATGTATAC CACATCATCT AAGGATCAAA GTTCTGATCG TACCAGTCTT 
TCTTCAGTGG GAGCCCAGGA TTCTGAATCT ACCTCTTTAA CAGATGAAGA TGTTTGCCAT 
GAATTGGAAG GAGCCACTTC CTCCCAAGAG AGCTGTGCTG CTTCAGGGAA TAAGGGAATT 
GATTTCAGCA GAACAAGCCT GGAAAGTTCT GCCTCCTTAG AAGGATCCCT GTCAAAGTTT 
GCCTTACCTG GGAAATCAGA AGTGGCATCT TCCTGCAACA CAAGTAATAC AAATATCTTC 
CAGAACTATG CAATGGAGGT TCTTATCTCA AGTTGCTCTC GGTGTAGAAC TTGTGATTGT 
CTTGTCCATG ATGAGGAAAT CATGGCTGGC TGGACAGCAG ATGATTCAAA TCTCAATACT 
ACATGCCCGT TTTGTGGCAA TCTCTTCTTG CCCTTTCTGA ATATTGAAAT AAGAGATTTA 
AGACGACCCG GAAGATACTT TCTGAAGTCA AGTCCATCTA CAGAAAATAT GCATTTTCCA 
TCCTCCCTTT CAAGTCAGAC AAGGCAGTCT TGTATCTCAA CATCAGCCTC TGGTCTTGAC 
ACGTCTGTTA TCTCTGTTCA AGGGAATTTT GATCTCAATA GCAAATCTAA ACTGCTGGAA 
AATACATGTG CCCGAACTAT TCACATCCCT GCTGATAGAT CAAAAACAGG TGTGTCAAAA 
TGTCCAATAT TTCCAATGGC CAGGAGTGTT AGTACTTATG GCCCCTTGGA TAAGGAAGAC 
ACTGGAGGCC AGAAGCTCAT TCCTACAGGC AGCCTGCCAA CTACTTTACA AGGAGCTACA 
GATTCTTTAG GATTAGAGTG GCACCTTCCT AGTCCAGATC CTATCACAGT TCCATATCTT 
AGTCCTTTAG TGGTATGGAA GGAGCTTGAA AGCTTACTGG AAAATGAAGG CGACCATGCA 
ATAACAGTGG CAGACTTTGT GGATCATCAT CCAATCGTGT TTTGGAACCT GGTGTGGTAT 
TTTAGACGTC TCGACTTACC CAGTAACTTA CCTGGATTGA TTCTTTCTTC TGAGCACTGT 
AACAAGTATT CAAAGATTCC TCGCCACTGT ATGTCGGAAG ATAGTAAATA CGTTTTAATA 
CAAATGTTAT GGGACAATAT GAAATTACAT CAGGATCCGG GACAGCCTTT GTACATCCTC 
TGGAATGCCC ACACCCAAAA GTATCCAATG GTCCATTTAT TGCAAAAGGG TGATAATTCA 
TTTAACCAGG AACTGTTGAA AAATATGGTA AAAAGCATTA AAATGAATGA TGTCTATGGA 
CCAATGAGTC AAATTTTAGA GACACTGAAT AAATGTCCTC ATTTTAAAAG ACAGAGGAGT 
TTATATAGAG AAATACTATT TCTCTCACTT GTGGCACTGG GAAGAGAAAA CATTGATATA 
GATGCATTTG ATAAAGAATA CAAGATGGCA TACGATCGTC TCACACCTAG TCAAGTCAAG 
AGTACACACA ACTGTGATAG ACCACCAAGT ACAGGGGTGA TGGAATGTCG AAAAACCTTT 
GGAGAACCTT ATCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_544733.3
CDS	342..5939
Translation	MIEDKGPRVADYFVVAGLTDVSKPLEEEIHFNDACHKVAKPKEPITDVSVIIKSLGEEVPRDYICIDVTPTGLSADLNNGSLVGPQIYLCYRRGRDKPPLTDLGVLYDWKERLKQGCEIIQSTPYGHPANISGSTSSQKIYITYRRASENMTQNTLAVTDICIIIPSKGESPPHTFCKVDKNLNNSMWGSAVYLCYKKSVAKTNTISYKAGLICRYPQEDYESFSLPESVPLFCLPMGATIECWPPNSKYPLPVFSTFVLTGASAEKVYGAAIQFYEPYSEENLTEKQRLLLGLASSADGKSDSSKTIHTSKCICLLSHWPFFDAFRKFLTFLYRYSISGPHVLPIEKHISHFMHKVPFPSPQRPRILVQLSPHDNLILSQPVSSPLPLSGGKFSTLLQNLGPENAVTLLVFAVTEHKILIHSLRPSVLTSVTEALVSMIFPFHWPCPYVPLCPLALADVLSAPCPFIVGIDSRYFDLYDPPPDVSCVDLDTNTISQTGDKKNVAWKILPKKPCKNLMNTLNNLHQQLAKLQQRPRDDGLMDLAMNDYDFNSGKRLHMIDLEIQEAFLCFMASILKGYRSYLRPITQAPSETATDASSLFALQAFLRSRDRSHQKFYNMMTKTQMFIRFIEECSFVSDKDASLAFFDDCVDKVDMDKSGEMRLIELDESFKSEHTVFVTPPEIPHLPNGEEPPLQYSYNGFPVLRNNLFERPEGFLQTRKNKLPSKSSSPSSPSPMFRRTKQEIKSAHKIAKRYSSIPQMWSRCLLRHCYGLWFICLPAYVKVCHSKVRALKTAYDVLKKMQSKKMDPPDEVCYRILMQLCGQYDQPVLAVRVLFEMQKAGIDPNAITYGYYNKAVLESTWPSRSRSGYFLWTKVRNVVLGVAQFKRALKKQAHLSQTTLSGGQSDLGYNSLSKDEVRRGDISTEEIQEEKDKKGSDSSSLSESESTKGSADCLPKLNYQSSSSIVRLSGTNNNGADKISGESTESTSKLLLLSSFEGTNETGNSQNRCFRKRHKSDNEPNLQQRLSWGSRNRNLSGGVLMGLMLNRINQEANPGDMVEKLGADAKILSNVISQSTRPTSLDIGKPPLRSKRDSLEKESSDDDTPFDGSNCLADKVDSPVIFDLEDLDSETDGSKVGYIASQNPRRIQRMSSSFSVKPSEKTDVATGFDPLSLLVAETEQQQKEEEEEDDEDSKSVSTPSARRDLAEEIVMYMNNMSSPLTSRTPSIDLQRACDDKSTSKKSPTFVKACRRSSLPPHSPRPVRLTKSKSYTKTEERPRDRLWSSPAFSPTCPFREESQDALTHSSPSFNLDTLLVPKLDVLRNSMFTAGKGVAEKASKWYSRFTMYTTSSKDQSSDRTSLSSVGAQDSESTSLTDEDVCHELEGATSSQESCAASGNKGIDFSRTSLESSASLEGSLSKFALPGKSEVASSCNTSNTNIFQNYAMEVLISSCSRCRTCDCLVHDEEIMAGWTADDSNLNTTCPFCGNLFLPFLNIEIRDLRRPGRYFLKSSPSTENMHFPSSLSSQTRQSCISTSASGLDTSVISVQGNFDLNSKSKLLENTCARTIHIPADRSKTGVSKCPIFPMARSVSTYGPLDKEDTGGQKLIPTGSLPTTLQGATDSLGLEWHLPSPDPITVPYLSPLVVWKELESLLENEGDHAITVADFVDHHPIVFWNLVWYFRRLDLPSNLPGLILSSEHCNKYSKIPRHCMSEDSKYVLIQMLWDNMKLHQDPGQPLYILWNAHTQKYPMVHLLQKGDNSFNQELLKNMVKSIKMNDVYGPMSQILETLNKCPHFKRQRSLYREILFLSLVALGRENIDIDAFDKEYKMAYDRLTPSQVKSTHNCDRPPSTGVMECRKTFGEPYL

Target ORF information:

RefSeq Version	XM_544733.6
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris DENN domain containing 4A (DENND4A), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_544733.6

ORF Insert Sequence:

ATGATTGAAG ATAAGGGTCC TCGTGTTGCT GACTACTTTG TTGTAGCAGG ATTAACTGAT 
GTTTCAAAGC CTCTTGAAGA AGAAATTCAC TTCAATGATG CCTGTCATAA AGTAGCTAAA 
CCAAAAGAAC CTATCACAGA TGTTTCGGTG ATTATTAAAT CTCTTGGGGA AGAAGTGCCA 
CGGGATTATA TATGTATTGA TGTTACCCCA ACTGGATTGT CGGCTGATCT CAATAATGGA 
AGTCTTGTGG GGCCACAGAT TTACCTTTGC TACAGAAGAG GAAGAGATAA GCCTCCACTT 
ACAGATCTGG GGGTTTTGTA TGACTGGAAA GAAAGATTGA AACAGGGATG TGAAATTATT 
CAGAGTACTC CCTATGGACA CCCTGCAAAT ATTAGTGGCA GTACCTCATC ACAAAAAATT 
TATATAACTT ACCGAAGAGC CTCTGAAAAC ATGACGCAAA ATACGTTGGC TGTCACAGAC 
ATATGTATTA TTATACCCAG TAAAGGAGAG AGTCCACCAC ACACGTTTTG CAAAGTCGAT 
AAGAATCTCA ATAACAGTAT GTGGGGATCT GCAGTGTACT TATGTTATAA AAAATCAGTG 
GCAAAGACTA ACACTATATC CTATAAAGCT GGTCTAATTT GTAGATATCC TCAAGAAGAT 
TACGAATCTT TCTCACTCCC AGAATCTGTT CCCCTATTTT GTTTACCAAT GGGTGCAACT 
ATTGAATGTT GGCCACCAAA TAGCAAATAC CCTCTCCCTG TATTTTCTAC ATTTGTGTTA 
ACTGGAGCCT CAGCTGAAAA GGTCTATGGT GCTGCTATTC AGTTTTATGA ACCATACTCT 
GAGGAGAATC TCACAGAAAA ACAGAGACTT CTTTTGGGTT TAGCATCATC AGCAGATGGG 
AAATCTGATA GTTCTAAAAC AATTCACACT AGTAAATGCA TCTGTCTTCT TTCTCACTGG 
CCTTTTTTTG ATGCATTCAG GAAATTTTTG ACCTTTCTGT ATCGTTACTC TATCTCTGGA 
CCTCATGTCC TTCCAATTGA GAAGCATATT TCTCATTTTA TGCATAAAGT TCCTTTTCCA 
TCTCCTCAGA GGCCACGGAT TTTAGTTCAG TTATCGCCAC ATGATAATCT GATTCTCAGT 
CAACCTGTTT CTTCACCTCT TCCACTAAGT GGTGGCAAAT TTTCAACACT ACTTCAAAAT 
TTAGGCCCTG AAAATGCTGT AACACTACTG GTGTTTGCAG TAACAGAGCA CAAGATTCTC 
ATCCATTCCT TACGGCCTTC CGTGCTTACT AGCGTGACAG AAGCATTAGT GTCTATGATT 
TTCCCTTTCC ACTGGCCGTG CCCATATGTT CCTCTCTGCC CACTGGCTTT AGCAGATGTC 
TTGAGTGCAC CATGTCCATT CATTGTAGGA ATTGATTCAA GATATTTTGA TCTCTATGAC 
CCTCCACCAG ATGTTAGTTG TGTTGACCTA GATACCAACA CTATTTCTCA AACTGGCGAT 
AAGAAAAATG TAGCCTGGAA GATACTTCCA AAGAAACCAT GTAAAAACCT GATGAACACA 
CTGAATAATT TGCACCAGCA GTTGGCAAAA TTGCAGCAGA GACCAAGAGA TGATGGACTA 
ATGGACTTAG CAATGAATGA CTATGATTTT AATTCTGGAA AGAGGTTGCA TATGATAGAT 
TTGGAAATCC AAGAGGCATT TTTGTGTTTC ATGGCCTCTA TTTTAAAAGG TTATAGATCA 
TACTTAAGAC CAATAACACA AGCCCCTTCT GAGACAGCCA CCGATGCATC CTCTCTTTTT 
GCCCTACAAG CTTTCTTAAG AAGCCGAGAC CGGTCACATC AAAAATTCTA TAACATGATG 
ACCAAAACAC AAATGTTCAT TCGCTTCATT GAGGAATGTT CTTTTGTCAG TGATAAAGAT 
GCAAGCCTGG CATTTTTTGA TGATTGTGTA GATAAAGTGG ATATGGACAA GAGTGGTGAA 
ATGCGACTTA TAGAACTGGA TGAATCTTTC AAAAGTGAAC ATACTGTTTT TGTAACACCA 
CCTGAGATAC CCCATCTACC CAATGGTGAA GAACCCCCTT TACAGTACAG CTATAATGGA 
TTTCCAGTTC TTAGAAACAA TTTATTTGAG AGACCTGAAG GATTTCTACA AACACGAAAG 
AACAAATTGC CTTCAAAATC AAGTAGTCCT AGTAGCCCTT CTCCCATGTT CAGAAGAACA 
AAACAGGAAA TTAAATCAGC CCATAAAATT GCCAAGAGGT ACTCTTCCAT TCCTCAGATG 
TGGTCTAGGT GTCTGCTGCG CCACTGCTAT GGACTGTGGT TCATTTGTCT GCCAGCTTAT 
GTGAAAGTGT GTCATTCAAA AGTCAGGGCA CTGAAGACAG CATATGATGT GCTCAAAAAA 
ATGCAGTCGA AAAAGATGGA TCCACCTGAT GAAGTGTGCT ACCGCATTCT CATGCAGCTC 
TGTGGGCAGT ACGATCAGCC CGTGCTTGCA GTTCGAGTGC TTTTTGAAAT GCAGAAAGCT 
GGTATTGATC CCAATGCCAT TACTTATGGT TATTATAATA AGGCTGTTTT GGAAAGCACC 
TGGCCCTCAA GAAGTCGTAG TGGCTATTTT CTTTGGACAA AAGTAAGAAA CGTTGTTTTA 
GGAGTAGCAC AGTTCAAAAG AGCTTTAAAG AAACAGGCAC ACTTATCACA AACAACTCTC 
TCAGGTGGCC AGTCTGATCT TGGATATAAT TCATTATCTA AGGATGAAGT TAGAAGAGGG 
GATATATCTA CTGAGGAAAT TCAAGAAGAA AAAGATAAGA AAGGAAGTGA TTCTAGTTCC 
CTGTCAGAGA GTGAGAGTAC AAAAGGAAGT GCTGACTGTC TTCCTAAACT CAATTATCAA 
AGTTCTTCCA GTATAGTTCG CCTCAGTGGT ACAAATAACA ATGGTGCTGA TAAAATATCA 
GGAGAAAGCA CAGAAAGCAC ATCTAAGCTG CTCTTACTAT CATCTTTTGA GGGTACAAAT 
GAAACAGGAA ATAGTCAAAA TAGATGCTTC AGGAAAAGAC ATAAAAGTGA CAACGAACCT 
AATTTGCAAC AGCGACTCTC CTGGGGAAGC AGAAACCGCA ATCTTAGTGG AGGAGTGCTG 
ATGGGACTTA TGCTTAATAG AATCAACCAG GAAGCAAACC CTGGAGACAT GGTTGAAAAA 
CTAGGAGCTG ATGCAAAAAT TCTTTCAAAC GTTATATCAC AAAGCACAAG ACCAACTAGT 
CTTGATATTG GAAAACCCCC TTTAAGATCA AAAAGAGACA GTCTAGAAAA GGAATCTAGC 
GACGATGATA CACCTTTCGA TGGTTCTAAT TGTTTGGCAG ATAAAGTGGA TTCTCCTGTT 
ATTTTTGATT TGGAAGACTT AGACAGTGAG ACAGATGGAT CAAAAGTGGG ATACATTGCT 
TCACAAAATC CCAGAAGGAT TCAGCGTATG AGCAGCAGCT TCTCGGTAAA ACCTTCTGAA 
AAAACGGATG TTGCCACAGG ATTTGATCCC CTCTCTCTTT TGGTTGCTGA GACTGAACAG 
CAGCAAAAAG AGGAAGAGGA GGAGGATGAT GAAGATAGCA AAAGCGTTTC AACACCATCC 
GCCAGGCGTG ACTTAGCCGA AGAGATTGTG ATGTATATGA ATAACATGAG CAGTCCTTTG 
ACAAGCCGCA CACCAAGCAT CGATTTACAA CGGGCATGTG ATGACAAATC AACCAGTAAG 
AAAAGTCCAA CATTTGTCAA GGCATGCAGG AGATCTAGCC TGCCTCCTCA TTCTCCGAGG 
CCAGTGAGAC TTACCAAATC TAAAAGTTAT ACAAAAACTG AGGAGAGACC TCGGGACAGA 
CTCTGGTCTT CTCCAGCCTT CTCACCTACT TGCCCATTTA GGGAAGAATC TCAAGACGCA 
TTAACCCATT CATCACCTTC ATTTAATTTA GATACACTGC TAGTACCTAA ACTAGATGTT 
CTAAGGAACA GTATGTTCAC TGCTGGGAAA GGAGTTGCCG AGAAAGCCAG CAAATGGTAT 
TCAAGATTCA CCATGTATAC CACATCATCT AAGGATCAAA GTTCTGATCG TACCAGTCTT 
TCTTCAGTGG GAGCCCAGGA TTCTGAATCT ACCTCTTTAA CAGATGAAGA TGTTTGCCAT 
GAATTGGAAG GAGCCACTTC CTCCCAAGAG AGCTGTGCTG CTTCAGGGAA TAAGGGAATT 
GATTTCAGCA GAACAAGCCT GGAAAGTTCT GCCTCCTTAG AAGGATCCCT GTCAAAGTTT 
GCCTTACCTG GGAAATCAGA AGTGGCATCT TCCTGCAACA CAAGTAATAC AAATATCTTC 
CAGAACTATG CAATGGAGGT TCTTATCTCA AGTTGCTCTC GGTGTAGAAC TTGTGATTGT 
CTTGTCCATG ATGAGGAAAT CATGGCTGGC TGGACAGCAG ATGATTCAAA TCTCAATACT 
ACATGCCCGT TTTGTGGCAA TCTCTTCTTG CCCTTTCTGA ATATTGAAAT AAGAGATTTA 
AGACGACCCG GAAGATACTT TCTGAAGTCA AGTCCATCTA CAGAAAATAT GCATTTTCCA 
TCCTCCCTTT CAAGTCAGAC AAGGCAGTCT TGTATCTCAA CATCAGCCTC TGGTCTTGAC 
ACGTCTGTTA TCTCTGTTCA AGGGAATTTT GATCTCAATA GCAAATCTAA ACTGCTGGAA 
AATACATGTG CCCGAACTAT TCACATCCCT GCTGATAGAT CAAAAACAGG TGTGTCAAAA 
TGTCCAATAT TTCCAATGGC CAGGAGTGTT AGTACTTATG GCCCCTTGGA TAAGGAAGAC 
ACTGGAGGCC AGAAGCTCAT TCCTACAGGC AGCCTGCCAA CTACTTTACA AGGAGCTACA 
GATTCTTTAG GATTAGAGTG GCACCTTCCT AGTCCAGATC CTATCACAGT TCCATATCTT 
AGTCCTTTAG TGGTATGGAA GGAGCTTGAA AGCTTACTGG AAAATGAAGG CGACCATGCA 
ATAACAGTGG CAGACTTTGT GGATCATCAT CCAATCGTGT TTTGGAACCT GGTGTGGTAT 
TTTAGACGTC TCGACTTACC CAGTAACTTA CCTGGATTGA TTCTTTCTTC TGAGCACTGT 
AACAAGTATT CAAAGATTCC TCGCCACTGT ATGTCGGAAG ATAGTAAATA CGTTTTAATA 
CAAATGTTAT GGGACAATAT GAAATTACAT CAGGATCCGG GACAGCCTTT GTACATCCTC 
TGGAATGCCC ACACCCAAAA GTATCCAATG GTCCATTTAT TGCAAAAGGG TGATAATTCA 
TTTAACCAGG AACTGTTGAA AAATATGGTA AAAAGCATTA AAATGAATGA TGTCTATGGA 
CCAATGAGTC AAATTTTAGA GACACTGAAT AAATGTCCTC ATTTTAAAAG ACAGAGGAGT 
TTATATAGAG AAATACTATT TCTCTCACTT GTGGCACTGG GAAGAGAAAA CATTGATATA 
GATGCATTTG ATAAAGAATA CAAGATGGCA TACGATCGTC TCACACCTAG TCAAGTCAAG 
AGTACACACA ACTGTGATAG ACCACCAAGT ACAGGGGTGA TGGAATGTCG AAAAACCTTT 
GGAGAACCTT ATCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DENND4A ( XM_544733.5 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_544733.3 Canis lupus familiaris DENN/MADD domain containing 4A (DENND4A), transcript variant X6, mRNA.

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CloneID	OCe29023
Clone ID Related Accession (Same CDS sequence)	XM_544733.6 , XM_544733.5
Accession Version	XM_544733.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5598bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	C-myc promoter-binding protein isoform X6
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726113.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_544733.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTGAAG ATAAGGGTCC TCGTGTTGCT GACTACTTTG TTGTAGCAGG ATTAACTGAT 
GTTTCAAAGC CTCTTGAAGA AGAAATTCAC TTCAATGATG CCTGTCATAA AGTAGCTAAA 
CCAAAAGAAC CTATCACAGA TGTTTCGGTG ATTATTAAAT CTCTTGGGGA AGAAGTGCCA 
CGGGATTATA TATGTATTGA TGTTACCCCA ACTGGATTGT CGGCTGATCT CAATAATGGA 
AGTCTTGTGG GGCCACAGAT TTACCTTTGC TACAGAAGAG GAAGAGATAA GCCTCCACTT 
ACAGATCTGG GGGTTTTGTA TGACTGGAAA GAAAGATTGA AACAGGGATG TGAAATTATT 
CAGAGTACTC CCTATGGACA CCCTGCAAAT ATTAGTGGCA GTACCTCATC ACAAAAAATT 
TATATAACTT ACCGAAGAGC CTCTGAAAAC ATGACGCAAA ATACGTTGGC TGTCACAGAC 
ATATGTATTA TTATACCCAG TAAAGGAGAG AGTCCACCAC ACACGTTTTG CAAAGTCGAT 
AAGAATCTCA ATAACAGTAT GTGGGGATCT GCAGTGTACT TATGTTATAA AAAATCAGTG 
GCAAAGACTA ACACTATATC CTATAAAGCT GGTCTAATTT GTAGATATCC TCAAGAAGAT 
TACGAATCTT TCTCACTCCC AGAATCTGTT CCCCTATTTT GTTTACCAAT GGGTGCAACT 
ATTGAATGTT GGCCACCAAA TAGCAAATAC CCTCTCCCTG TATTTTCTAC ATTTGTGTTA 
ACTGGAGCCT CAGCTGAAAA GGTCTATGGT GCTGCTATTC AGTTTTATGA ACCATACTCT 
GAGGAGAATC TCACAGAAAA ACAGAGACTT CTTTTGGGTT TAGCATCATC AGCAGATGGG 
AAATCTGATA GTTCTAAAAC AATTCACACT AGTAAATGCA TCTGTCTTCT TTCTCACTGG 
CCTTTTTTTG ATGCATTCAG GAAATTTTTG ACCTTTCTGT ATCGTTACTC TATCTCTGGA 
CCTCATGTCC TTCCAATTGA GAAGCATATT TCTCATTTTA TGCATAAAGT TCCTTTTCCA 
TCTCCTCAGA GGCCACGGAT TTTAGTTCAG TTATCGCCAC ATGATAATCT GATTCTCAGT 
CAACCTGTTT CTTCACCTCT TCCACTAAGT GGTGGCAAAT TTTCAACACT ACTTCAAAAT 
TTAGGCCCTG AAAATGCTGT AACACTACTG GTGTTTGCAG TAACAGAGCA CAAGATTCTC 
ATCCATTCCT TACGGCCTTC CGTGCTTACT AGCGTGACAG AAGCATTAGT GTCTATGATT 
TTCCCTTTCC ACTGGCCGTG CCCATATGTT CCTCTCTGCC CACTGGCTTT AGCAGATGTC 
TTGAGTGCAC CATGTCCATT CATTGTAGGA ATTGATTCAA GATATTTTGA TCTCTATGAC 
CCTCCACCAG ATGTTAGTTG TGTTGACCTA GATACCAACA CTATTTCTCA AACTGGCGAT 
AAGAAAAATG TAGCCTGGAA GATACTTCCA AAGAAACCAT GTAAAAACCT GATGAACACA 
CTGAATAATT TGCACCAGCA GTTGGCAAAA TTGCAGCAGA GACCAAGAGA TGATGGACTA 
ATGGACTTAG CAATGAATGA CTATGATTTT AATTCTGGAA AGAGGTTGCA TATGATAGAT 
TTGGAAATCC AAGAGGCATT TTTGTGTTTC ATGGCCTCTA TTTTAAAAGG TTATAGATCA 
TACTTAAGAC CAATAACACA AGCCCCTTCT GAGACAGCCA CCGATGCATC CTCTCTTTTT 
GCCCTACAAG CTTTCTTAAG AAGCCGAGAC CGGTCACATC AAAAATTCTA TAACATGATG 
ACCAAAACAC AAATGTTCAT TCGCTTCATT GAGGAATGTT CTTTTGTCAG TGATAAAGAT 
GCAAGCCTGG CATTTTTTGA TGATTGTGTA GATAAAGTGG ATATGGACAA GAGTGGTGAA 
ATGCGACTTA TAGAACTGGA TGAATCTTTC AAAAGTGAAC ATACTGTTTT TGTAACACCA 
CCTGAGATAC CCCATCTACC CAATGGTGAA GAACCCCCTT TACAGTACAG CTATAATGGA 
TTTCCAGTTC TTAGAAACAA TTTATTTGAG AGACCTGAAG GATTTCTACA AACACGAAAG 
AACAAATTGC CTTCAAAATC AAGTAGTCCT AGTAGCCCTT CTCCCATGTT CAGAAGAACA 
AAACAGGAAA TTAAATCAGC CCATAAAATT GCCAAGAGGT ACTCTTCCAT TCCTCAGATG 
TGGTCTAGGT GTCTGCTGCG CCACTGCTAT GGACTGTGGT TCATTTGTCT GCCAGCTTAT 
GTGAAAGTGT GTCATTCAAA AGTCAGGGCA CTGAAGACAG CATATGATGT GCTCAAAAAA 
ATGCAGTCGA AAAAGATGGA TCCACCTGAT GAAGTGTGCT ACCGCATTCT CATGCAGCTC 
TGTGGGCAGT ACGATCAGCC CGTGCTTGCA GTTCGAGTGC TTTTTGAAAT GCAGAAAGCT 
GGTATTGATC CCAATGCCAT TACTTATGGT TATTATAATA AGGCTGTTTT GGAAAGCACC 
TGGCCCTCAA GAAGTCGTAG TGGCTATTTT CTTTGGACAA AAGTAAGAAA CGTTGTTTTA 
GGAGTAGCAC AGTTCAAAAG AGCTTTAAAG AAACAGGCAC ACTTATCACA AACAACTCTC 
TCAGGTGGCC AGTCTGATCT TGGATATAAT TCATTATCTA AGGATGAAGT TAGAAGAGGG 
GATATATCTA CTGAGGAAAT TCAAGAAGAA AAAGATAAGA AAGGAAGTGA TTCTAGTTCC 
CTGTCAGAGA GTGAGAGTAC AAAAGGAAGT GCTGACTGTC TTCCTAAACT CAATTATCAA 
AGTTCTTCCA GTATAGTTCG CCTCAGTGGT ACAAATAACA ATGGTGCTGA TAAAATATCA 
GGAGAAAGCA CAGAAAGCAC ATCTAAGCTG CTCTTACTAT CATCTTTTGA GGGTACAAAT 
GAAACAGGAA ATAGTCAAAA TAGATGCTTC AGGAAAAGAC ATAAAAGTGA CAACGAACCT 
AATTTGCAAC AGCGACTCTC CTGGGGAAGC AGAAACCGCA ATCTTAGTGG AGGAGTGCTG 
ATGGGACTTA TGCTTAATAG AATCAACCAG GAAGCAAACC CTGGAGACAT GGTTGAAAAA 
CTAGGAGCTG ATGCAAAAAT TCTTTCAAAC GTTATATCAC AAAGCACAAG ACCAACTAGT 
CTTGATATTG GAAAACCCCC TTTAAGATCA AAAAGAGACA GTCTAGAAAA GGAATCTAGC 
GACGATGATA CACCTTTCGA TGGTTCTAAT TGTTTGGCAG ATAAAGTGGA TTCTCCTGTT 
ATTTTTGATT TGGAAGACTT AGACAGTGAG ACAGATGGAT CAAAAGTGGG ATACATTGCT 
TCACAAAATC CCAGAAGGAT TCAGCGTATG AGCAGCAGCT TCTCGGTAAA ACCTTCTGAA 
AAAACGGATG TTGCCACAGG ATTTGATCCC CTCTCTCTTT TGGTTGCTGA GACTGAACAG 
CAGCAAAAAG AGGAAGAGGA GGAGGATGAT GAAGATAGCA AAAGCGTTTC AACACCATCC 
GCCAGGCGTG ACTTAGCCGA AGAGATTGTG ATGTATATGA ATAACATGAG CAGTCCTTTG 
ACAAGCCGCA CACCAAGCAT CGATTTACAA CGGGCATGTG ATGACAAATC AACCAGTAAG 
AAAAGTCCAA CATTTGTCAA GGCATGCAGG AGATCTAGCC TGCCTCCTCA TTCTCCGAGG 
CCAGTGAGAC TTACCAAATC TAAAAGTTAT ACAAAAACTG AGGAGAGACC TCGGGACAGA 
CTCTGGTCTT CTCCAGCCTT CTCACCTACT TGCCCATTTA GGGAAGAATC TCAAGACGCA 
TTAACCCATT CATCACCTTC ATTTAATTTA GATACACTGC TAGTACCTAA ACTAGATGTT 
CTAAGGAACA GTATGTTCAC TGCTGGGAAA GGAGTTGCCG AGAAAGCCAG CAAATGGTAT 
TCAAGATTCA CCATGTATAC CACATCATCT AAGGATCAAA GTTCTGATCG TACCAGTCTT 
TCTTCAGTGG GAGCCCAGGA TTCTGAATCT ACCTCTTTAA CAGATGAAGA TGTTTGCCAT 
GAATTGGAAG GAGCCACTTC CTCCCAAGAG AGCTGTGCTG CTTCAGGGAA TAAGGGAATT 
GATTTCAGCA GAACAAGCCT GGAAAGTTCT GCCTCCTTAG AAGGATCCCT GTCAAAGTTT 
GCCTTACCTG GGAAATCAGA AGTGGCATCT TCCTGCAACA CAAGTAATAC AAATATCTTC 
CAGAACTATG CAATGGAGGT TCTTATCTCA AGTTGCTCTC GGTGTAGAAC TTGTGATTGT 
CTTGTCCATG ATGAGGAAAT CATGGCTGGC TGGACAGCAG ATGATTCAAA TCTCAATACT 
ACATGCCCGT TTTGTGGCAA TCTCTTCTTG CCCTTTCTGA ATATTGAAAT AAGAGATTTA 
AGACGACCCG GAAGATACTT TCTGAAGTCA AGTCCATCTA CAGAAAATAT GCATTTTCCA 
TCCTCCCTTT CAAGTCAGAC AAGGCAGTCT TGTATCTCAA CATCAGCCTC TGGTCTTGAC 
ACGTCTGTTA TCTCTGTTCA AGGGAATTTT GATCTCAATA GCAAATCTAA ACTGCTGGAA 
AATACATGTG CCCGAACTAT TCACATCCCT GCTGATAGAT CAAAAACAGG TGTGTCAAAA 
TGTCCAATAT TTCCAATGGC CAGGAGTGTT AGTACTTATG GCCCCTTGGA TAAGGAAGAC 
ACTGGAGGCC AGAAGCTCAT TCCTACAGGC AGCCTGCCAA CTACTTTACA AGGAGCTACA 
GATTCTTTAG GATTAGAGTG GCACCTTCCT AGTCCAGATC CTATCACAGT TCCATATCTT 
AGTCCTTTAG TGGTATGGAA GGAGCTTGAA AGCTTACTGG AAAATGAAGG CGACCATGCA 
ATAACAGTGG CAGACTTTGT GGATCATCAT CCAATCGTGT TTTGGAACCT GGTGTGGTAT 
TTTAGACGTC TCGACTTACC CAGTAACTTA CCTGGATTGA TTCTTTCTTC TGAGCACTGT 
AACAAGTATT CAAAGATTCC TCGCCACTGT ATGTCGGAAG ATAGTAAATA CGTTTTAATA 
CAAATGTTAT GGGACAATAT GAAATTACAT CAGGATCCGG GACAGCCTTT GTACATCCTC 
TGGAATGCCC ACACCCAAAA GTATCCAATG GTCCATTTAT TGCAAAAGGG TGATAATTCA 
TTTAACCAGG AACTGTTGAA AAATATGGTA AAAAGCATTA AAATGAATGA TGTCTATGGA 
CCAATGAGTC AAATTTTAGA GACACTGAAT AAATGTCCTC ATTTTAAAAG ACAGAGGAGT 
TTATATAGAG AAATACTATT TCTCTCACTT GTGGCACTGG GAAGAGAAAA CATTGATATA 
GATGCATTTG ATAAAGAATA CAAGATGGCA TACGATCGTC TCACACCTAG TCAAGTCAAG 
AGTACACACA ACTGTGATAG ACCACCAAGT ACAGGGGTGA TGGAATGTCG AAAAACCTTT 
GGAGAACCTT ATCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_544733.3
CDS	378..5975
Translation	MIEDKGPRVADYFVVAGLTDVSKPLEEEIHFNDACHKVAKPKEPITDVSVIIKSLGEEVPRDYICIDVTPTGLSADLNNGSLVGPQIYLCYRRGRDKPPLTDLGVLYDWKERLKQGCEIIQSTPYGHPANISGSTSSQKIYITYRRASENMTQNTLAVTDICIIIPSKGESPPHTFCKVDKNLNNSMWGSAVYLCYKKSVAKTNTISYKAGLICRYPQEDYESFSLPESVPLFCLPMGATIECWPPNSKYPLPVFSTFVLTGASAEKVYGAAIQFYEPYSEENLTEKQRLLLGLASSADGKSDSSKTIHTSKCICLLSHWPFFDAFRKFLTFLYRYSISGPHVLPIEKHISHFMHKVPFPSPQRPRILVQLSPHDNLILSQPVSSPLPLSGGKFSTLLQNLGPENAVTLLVFAVTEHKILIHSLRPSVLTSVTEALVSMIFPFHWPCPYVPLCPLALADVLSAPCPFIVGIDSRYFDLYDPPPDVSCVDLDTNTISQTGDKKNVAWKILPKKPCKNLMNTLNNLHQQLAKLQQRPRDDGLMDLAMNDYDFNSGKRLHMIDLEIQEAFLCFMASILKGYRSYLRPITQAPSETATDASSLFALQAFLRSRDRSHQKFYNMMTKTQMFIRFIEECSFVSDKDASLAFFDDCVDKVDMDKSGEMRLIELDESFKSEHTVFVTPPEIPHLPNGEEPPLQYSYNGFPVLRNNLFERPEGFLQTRKNKLPSKSSSPSSPSPMFRRTKQEIKSAHKIAKRYSSIPQMWSRCLLRHCYGLWFICLPAYVKVCHSKVRALKTAYDVLKKMQSKKMDPPDEVCYRILMQLCGQYDQPVLAVRVLFEMQKAGIDPNAITYGYYNKAVLESTWPSRSRSGYFLWTKVRNVVLGVAQFKRALKKQAHLSQTTLSGGQSDLGYNSLSKDEVRRGDISTEEIQEEKDKKGSDSSSLSESESTKGSADCLPKLNYQSSSSIVRLSGTNNNGADKISGESTESTSKLLLLSSFEGTNETGNSQNRCFRKRHKSDNEPNLQQRLSWGSRNRNLSGGVLMGLMLNRINQEANPGDMVEKLGADAKILSNVISQSTRPTSLDIGKPPLRSKRDSLEKESSDDDTPFDGSNCLADKVDSPVIFDLEDLDSETDGSKVGYIASQNPRRIQRMSSSFSVKPSEKTDVATGFDPLSLLVAETEQQQKEEEEEDDEDSKSVSTPSARRDLAEEIVMYMNNMSSPLTSRTPSIDLQRACDDKSTSKKSPTFVKACRRSSLPPHSPRPVRLTKSKSYTKTEERPRDRLWSSPAFSPTCPFREESQDALTHSSPSFNLDTLLVPKLDVLRNSMFTAGKGVAEKASKWYSRFTMYTTSSKDQSSDRTSLSSVGAQDSESTSLTDEDVCHELEGATSSQESCAASGNKGIDFSRTSLESSASLEGSLSKFALPGKSEVASSCNTSNTNIFQNYAMEVLISSCSRCRTCDCLVHDEEIMAGWTADDSNLNTTCPFCGNLFLPFLNIEIRDLRRPGRYFLKSSPSTENMHFPSSLSSQTRQSCISTSASGLDTSVISVQGNFDLNSKSKLLENTCARTIHIPADRSKTGVSKCPIFPMARSVSTYGPLDKEDTGGQKLIPTGSLPTTLQGATDSLGLEWHLPSPDPITVPYLSPLVVWKELESLLENEGDHAITVADFVDHHPIVFWNLVWYFRRLDLPSNLPGLILSSEHCNKYSKIPRHCMSEDSKYVLIQMLWDNMKLHQDPGQPLYILWNAHTQKYPMVHLLQKGDNSFNQELLKNMVKSIKMNDVYGPMSQILETLNKCPHFKRQRSLYREILFLSLVALGRENIDIDAFDKEYKMAYDRLTPSQVKSTHNCDRPPSTGVMECRKTFGEPYL

Target ORF information:

RefSeq Version	XM_544733.5
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris DENN/MADD domain containing 4A (DENND4A), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_544733.5

ORF Insert Sequence:

ATGATTGAAG ATAAGGGTCC TCGTGTTGCT GACTACTTTG TTGTAGCAGG ATTAACTGAT 
GTTTCAAAGC CTCTTGAAGA AGAAATTCAC TTCAATGATG CCTGTCATAA AGTAGCTAAA 
CCAAAAGAAC CTATCACAGA TGTTTCGGTG ATTATTAAAT CTCTTGGGGA AGAAGTGCCA 
CGGGATTATA TATGTATTGA TGTTACCCCA ACTGGATTGT CGGCTGATCT CAATAATGGA 
AGTCTTGTGG GGCCACAGAT TTACCTTTGC TACAGAAGAG GAAGAGATAA GCCTCCACTT 
ACAGATCTGG GGGTTTTGTA TGACTGGAAA GAAAGATTGA AACAGGGATG TGAAATTATT 
CAGAGTACTC CCTATGGACA CCCTGCAAAT ATTAGTGGCA GTACCTCATC ACAAAAAATT 
TATATAACTT ACCGAAGAGC CTCTGAAAAC ATGACGCAAA ATACGTTGGC TGTCACAGAC 
ATATGTATTA TTATACCCAG TAAAGGAGAG AGTCCACCAC ACACGTTTTG CAAAGTCGAT 
AAGAATCTCA ATAACAGTAT GTGGGGATCT GCAGTGTACT TATGTTATAA AAAATCAGTG 
GCAAAGACTA ACACTATATC CTATAAAGCT GGTCTAATTT GTAGATATCC TCAAGAAGAT 
TACGAATCTT TCTCACTCCC AGAATCTGTT CCCCTATTTT GTTTACCAAT GGGTGCAACT 
ATTGAATGTT GGCCACCAAA TAGCAAATAC CCTCTCCCTG TATTTTCTAC ATTTGTGTTA 
ACTGGAGCCT CAGCTGAAAA GGTCTATGGT GCTGCTATTC AGTTTTATGA ACCATACTCT 
GAGGAGAATC TCACAGAAAA ACAGAGACTT CTTTTGGGTT TAGCATCATC AGCAGATGGG 
AAATCTGATA GTTCTAAAAC AATTCACACT AGTAAATGCA TCTGTCTTCT TTCTCACTGG 
CCTTTTTTTG ATGCATTCAG GAAATTTTTG ACCTTTCTGT ATCGTTACTC TATCTCTGGA 
CCTCATGTCC TTCCAATTGA GAAGCATATT TCTCATTTTA TGCATAAAGT TCCTTTTCCA 
TCTCCTCAGA GGCCACGGAT TTTAGTTCAG TTATCGCCAC ATGATAATCT GATTCTCAGT 
CAACCTGTTT CTTCACCTCT TCCACTAAGT GGTGGCAAAT TTTCAACACT ACTTCAAAAT 
TTAGGCCCTG AAAATGCTGT AACACTACTG GTGTTTGCAG TAACAGAGCA CAAGATTCTC 
ATCCATTCCT TACGGCCTTC CGTGCTTACT AGCGTGACAG AAGCATTAGT GTCTATGATT 
TTCCCTTTCC ACTGGCCGTG CCCATATGTT CCTCTCTGCC CACTGGCTTT AGCAGATGTC 
TTGAGTGCAC CATGTCCATT CATTGTAGGA ATTGATTCAA GATATTTTGA TCTCTATGAC 
CCTCCACCAG ATGTTAGTTG TGTTGACCTA GATACCAACA CTATTTCTCA AACTGGCGAT 
AAGAAAAATG TAGCCTGGAA GATACTTCCA AAGAAACCAT GTAAAAACCT GATGAACACA 
CTGAATAATT TGCACCAGCA GTTGGCAAAA TTGCAGCAGA GACCAAGAGA TGATGGACTA 
ATGGACTTAG CAATGAATGA CTATGATTTT AATTCTGGAA AGAGGTTGCA TATGATAGAT 
TTGGAAATCC AAGAGGCATT TTTGTGTTTC ATGGCCTCTA TTTTAAAAGG TTATAGATCA 
TACTTAAGAC CAATAACACA AGCCCCTTCT GAGACAGCCA CCGATGCATC CTCTCTTTTT 
GCCCTACAAG CTTTCTTAAG AAGCCGAGAC CGGTCACATC AAAAATTCTA TAACATGATG 
ACCAAAACAC AAATGTTCAT TCGCTTCATT GAGGAATGTT CTTTTGTCAG TGATAAAGAT 
GCAAGCCTGG CATTTTTTGA TGATTGTGTA GATAAAGTGG ATATGGACAA GAGTGGTGAA 
ATGCGACTTA TAGAACTGGA TGAATCTTTC AAAAGTGAAC ATACTGTTTT TGTAACACCA 
CCTGAGATAC CCCATCTACC CAATGGTGAA GAACCCCCTT TACAGTACAG CTATAATGGA 
TTTCCAGTTC TTAGAAACAA TTTATTTGAG AGACCTGAAG GATTTCTACA AACACGAAAG 
AACAAATTGC CTTCAAAATC AAGTAGTCCT AGTAGCCCTT CTCCCATGTT CAGAAGAACA 
AAACAGGAAA TTAAATCAGC CCATAAAATT GCCAAGAGGT ACTCTTCCAT TCCTCAGATG 
TGGTCTAGGT GTCTGCTGCG CCACTGCTAT GGACTGTGGT TCATTTGTCT GCCAGCTTAT 
GTGAAAGTGT GTCATTCAAA AGTCAGGGCA CTGAAGACAG CATATGATGT GCTCAAAAAA 
ATGCAGTCGA AAAAGATGGA TCCACCTGAT GAAGTGTGCT ACCGCATTCT CATGCAGCTC 
TGTGGGCAGT ACGATCAGCC CGTGCTTGCA GTTCGAGTGC TTTTTGAAAT GCAGAAAGCT 
GGTATTGATC CCAATGCCAT TACTTATGGT TATTATAATA AGGCTGTTTT GGAAAGCACC 
TGGCCCTCAA GAAGTCGTAG TGGCTATTTT CTTTGGACAA AAGTAAGAAA CGTTGTTTTA 
GGAGTAGCAC AGTTCAAAAG AGCTTTAAAG AAACAGGCAC ACTTATCACA AACAACTCTC 
TCAGGTGGCC AGTCTGATCT TGGATATAAT TCATTATCTA AGGATGAAGT TAGAAGAGGG 
GATATATCTA CTGAGGAAAT TCAAGAAGAA AAAGATAAGA AAGGAAGTGA TTCTAGTTCC 
CTGTCAGAGA GTGAGAGTAC AAAAGGAAGT GCTGACTGTC TTCCTAAACT CAATTATCAA 
AGTTCTTCCA GTATAGTTCG CCTCAGTGGT ACAAATAACA ATGGTGCTGA TAAAATATCA 
GGAGAAAGCA CAGAAAGCAC ATCTAAGCTG CTCTTACTAT CATCTTTTGA GGGTACAAAT 
GAAACAGGAA ATAGTCAAAA TAGATGCTTC AGGAAAAGAC ATAAAAGTGA CAACGAACCT 
AATTTGCAAC AGCGACTCTC CTGGGGAAGC AGAAACCGCA ATCTTAGTGG AGGAGTGCTG 
ATGGGACTTA TGCTTAATAG AATCAACCAG GAAGCAAACC CTGGAGACAT GGTTGAAAAA 
CTAGGAGCTG ATGCAAAAAT TCTTTCAAAC GTTATATCAC AAAGCACAAG ACCAACTAGT 
CTTGATATTG GAAAACCCCC TTTAAGATCA AAAAGAGACA GTCTAGAAAA GGAATCTAGC 
GACGATGATA CACCTTTCGA TGGTTCTAAT TGTTTGGCAG ATAAAGTGGA TTCTCCTGTT 
ATTTTTGATT TGGAAGACTT AGACAGTGAG ACAGATGGAT CAAAAGTGGG ATACATTGCT 
TCACAAAATC CCAGAAGGAT TCAGCGTATG AGCAGCAGCT TCTCGGTAAA ACCTTCTGAA 
AAAACGGATG TTGCCACAGG ATTTGATCCC CTCTCTCTTT TGGTTGCTGA GACTGAACAG 
CAGCAAAAAG AGGAAGAGGA GGAGGATGAT GAAGATAGCA AAAGCGTTTC AACACCATCC 
GCCAGGCGTG ACTTAGCCGA AGAGATTGTG ATGTATATGA ATAACATGAG CAGTCCTTTG 
ACAAGCCGCA CACCAAGCAT CGATTTACAA CGGGCATGTG ATGACAAATC AACCAGTAAG 
AAAAGTCCAA CATTTGTCAA GGCATGCAGG AGATCTAGCC TGCCTCCTCA TTCTCCGAGG 
CCAGTGAGAC TTACCAAATC TAAAAGTTAT ACAAAAACTG AGGAGAGACC TCGGGACAGA 
CTCTGGTCTT CTCCAGCCTT CTCACCTACT TGCCCATTTA GGGAAGAATC TCAAGACGCA 
TTAACCCATT CATCACCTTC ATTTAATTTA GATACACTGC TAGTACCTAA ACTAGATGTT 
CTAAGGAACA GTATGTTCAC TGCTGGGAAA GGAGTTGCCG AGAAAGCCAG CAAATGGTAT 
TCAAGATTCA CCATGTATAC CACATCATCT AAGGATCAAA GTTCTGATCG TACCAGTCTT 
TCTTCAGTGG GAGCCCAGGA TTCTGAATCT ACCTCTTTAA CAGATGAAGA TGTTTGCCAT 
GAATTGGAAG GAGCCACTTC CTCCCAAGAG AGCTGTGCTG CTTCAGGGAA TAAGGGAATT 
GATTTCAGCA GAACAAGCCT GGAAAGTTCT GCCTCCTTAG AAGGATCCCT GTCAAAGTTT 
GCCTTACCTG GGAAATCAGA AGTGGCATCT TCCTGCAACA CAAGTAATAC AAATATCTTC 
CAGAACTATG CAATGGAGGT TCTTATCTCA AGTTGCTCTC GGTGTAGAAC TTGTGATTGT 
CTTGTCCATG ATGAGGAAAT CATGGCTGGC TGGACAGCAG ATGATTCAAA TCTCAATACT 
ACATGCCCGT TTTGTGGCAA TCTCTTCTTG CCCTTTCTGA ATATTGAAAT AAGAGATTTA 
AGACGACCCG GAAGATACTT TCTGAAGTCA AGTCCATCTA CAGAAAATAT GCATTTTCCA 
TCCTCCCTTT CAAGTCAGAC AAGGCAGTCT TGTATCTCAA CATCAGCCTC TGGTCTTGAC 
ACGTCTGTTA TCTCTGTTCA AGGGAATTTT GATCTCAATA GCAAATCTAA ACTGCTGGAA 
AATACATGTG CCCGAACTAT TCACATCCCT GCTGATAGAT CAAAAACAGG TGTGTCAAAA 
TGTCCAATAT TTCCAATGGC CAGGAGTGTT AGTACTTATG GCCCCTTGGA TAAGGAAGAC 
ACTGGAGGCC AGAAGCTCAT TCCTACAGGC AGCCTGCCAA CTACTTTACA AGGAGCTACA 
GATTCTTTAG GATTAGAGTG GCACCTTCCT AGTCCAGATC CTATCACAGT TCCATATCTT 
AGTCCTTTAG TGGTATGGAA GGAGCTTGAA AGCTTACTGG AAAATGAAGG CGACCATGCA 
ATAACAGTGG CAGACTTTGT GGATCATCAT CCAATCGTGT TTTGGAACCT GGTGTGGTAT 
TTTAGACGTC TCGACTTACC CAGTAACTTA CCTGGATTGA TTCTTTCTTC TGAGCACTGT 
AACAAGTATT CAAAGATTCC TCGCCACTGT ATGTCGGAAG ATAGTAAATA CGTTTTAATA 
CAAATGTTAT GGGACAATAT GAAATTACAT CAGGATCCGG GACAGCCTTT GTACATCCTC 
TGGAATGCCC ACACCCAAAA GTATCCAATG GTCCATTTAT TGCAAAAGGG TGATAATTCA 
TTTAACCAGG AACTGTTGAA AAATATGGTA AAAAGCATTA AAATGAATGA TGTCTATGGA 
CCAATGAGTC AAATTTTAGA GACACTGAAT AAATGTCCTC ATTTTAAAAG ACAGAGGAGT 
TTATATAGAG AAATACTATT TCTCTCACTT GTGGCACTGG GAAGAGAAAA CATTGATATA 
GATGCATTTG ATAAAGAATA CAAGATGGCA TACGATCGTC TCACACCTAG TCAAGTCAAG 
AGTACACACA ACTGTGATAG ACCACCAAGT ACAGGGGTGA TGGAATGTCG AAAAACCTTT 
GGAGAACCTT ATCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	DENND4A
Entrez Gene ID	487608
Full Name	DENN domain containing 4A
General protein information	Preferred Names DENN domain containing 4A Names C-myc promoter-binding protein
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

Homo sapiens (human)	DENND4A	NP_001138295.1
Bos taurus (cattle)	DENND4A	NP_001192454.1
Gallus gallus (chicken)	DENND4A	XP_413911.4
Drosophila melanogaster (fruit fly)	Crag	NP_727281.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001102	XP_322065.3
Pan troglodytes (chimpanzee)	DENND4A	XP_523102.2
	DENND4A	XP_001109950.1
Rattus norvegicus (Norway rat)	Dennd4a	XP_006243364.1
Mus musculus (house mouse)	Dennd4a	NP_001156389.1
Canis lupus familiaris (dog)	DENND4A	XP_005638584.1
Danio rerio (zebrafish)	dennd4a	NP_001074458.1
Xenopus tropicalis (tropical clawed frog)	dennd4a	XP_004912733.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

DENND4A cDNA ORF clone, Canis lupus familiaris(dog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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