F9 cDNA ORF clone, Canis lupus familiaris(dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	F9
Entrez Gene ID	404015
Full Name	coagulation factor IX
General protein information	Preferred Names coagulation factor IX Names coagulation factor IX christmas factor coagulation factor IX (plasma thromboplastic component, Christmas disease, hemophilia B) factor IX activated protein
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001003323.2	NP_001003323.1	coagulation factor IX preproprotein

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	F9	NP_032005.1
Rattus norvegicus (Norway rat)	F9	NP_113728.1
	F9	NP_001103153.1
Gallus gallus (chicken)	F9	NP_989674.1
Danio rerio (zebrafish)	f9b	NP_001035400.1
Danio rerio (zebrafish)	f9a	NP_878288.1
Xenopus tropicalis (tropical clawed frog)	f9	NP_001011223.1
Homo sapiens (human)	F9	NP_000124.1
Pan troglodytes (chimpanzee)	F9	NP_001129063.1
Canis lupus familiaris (dog)	F9	NP_001003323.1

Gene Ontology
Bibliography

Related articles in PubMed

The efficacy and the risk of immunogenicity of FIX Padua (R338L) in hemophilia B dogs treated by AAV muscle gene therapy.
Finn JD, Nichols TC, Svoronos N, Merricks EP, Bellenger DA, Zhou S, Simioni P, High KA, Arruda VR
Blood120(23)4521-3(2012 Nov)

Hyperfunctional coagulation factor IX improves the efficacy of gene therapy in hemophilic mice.
Cantore A, Nair N, Della Valle P, Di Matteo M, M?trai J, Sanvito F, Brombin C, Di Serio C, D'Angelo A, Chuah M, Naldini L, Vandendriessche T
Blood120(23)4517-20(2012 Nov)

Sensitivity of whole blood clotting time and activated partial thromboplastin time for factor IX: relevance to gene therapy and determination of post-transfusion elimination time of canine factor IX in hemophilia B dogs.
Nichols TC, Franck HW, Franck CT, De Friess N, Raymer RA, Merricks EP
Journal of thrombosis and haemostasis : JTH10(3)474-6(2012 Mar)

Successful in vivo propagation of factor IX-producing hepatocytes in mice: potential for cell-based therapy in haemophilia B.
Tatsumi K, Ohashi K, Kataoka M, Tateno C, Shibata M, Naka H, Shima M, Hisanaga M, Kanehiro H, Okano T, Yoshizato K, Nakajima Y, Yoshioka A
Thrombosis and haemostasis99(5)883-91(2008 May)

Molecular cloning of a cDNA encoding canine factor IX.
Evans JP, Watzke HH, Ware JL, Stafford DW, High KA
Blood74(1)207-12(1989 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data indicate that factor IX (FIX)-R338L provides an attractive strategy to safely enhance the efficacy of gene therapy for hemophilia B (HB).
Title: The efficacy and the risk of immunogenicity of FIX Padua (R338L) in hemophilia B dogs treated by AAV muscle gene therapy.

Data indicate that the hyperactivating factor IX (FIX)-R338L mutation resulted in a robust 15-fold gain in potency and therefore provides a promising strategy to improve the efficacy, feasibility, and safety of hemophilia gene therapy.
Title: Hyperfunctional coagulation factor IX improves the efficacy of gene therapy in hemophilic mice.

Report relative sensitivity of whole blood clotting time and activated partial thromboplastin time for the detection of factor IX.
Title: Sensitivity of whole blood clotting time and activated partial thromboplastin time for factor IX: relevance to gene therapy and determination of post-transfusion elimination time of canine factor IX in hemophilia B dogs.

Describe proliferation of FIX-producing dog hepatocytes in mouse liver.
Title: Successful in vivo propagation of factor IX-producing hepatocytes in mice: potential for cell-based therapy in haemophilia B.

The following F9 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the F9 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCe00419	NM_001003323.2 Latest version!	Canis lupus familiaris coagulation factor IX (F9), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

F9 ( NM_001003323.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> NP_001003323.1 Canis lupus familiaris coagulation factor IX (F9), mRNA.

Price & Availability↑

CloneID	OCe00419
Clone ID Related Accession (Same CDS sequence)	NM_001003323.2
Accession Version	NM_001003323.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1359bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Canis lupus familiaris(dog)
Product	coagulation factor IX preproprotein
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from M21757.1, GR893754.1, GR894491.1 and M33826.1. On Jun 21, 2011 this sequence version replaced NM_001003323.1. ##Evidence-Data-START## Transcript exon combination :: M33826.1, M21757.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00013515, SAMN07419780 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCAGAAG CATCGGGCCT CGTCACCGTC TGCCTTTTAG GATATCTACT CAGTGCCGAA 
TGTGCAGTTT TTCTTGATCG TGAAAATGCC ACCAAAATTC TGAGTCGGCC AAAGAGGTAT 
AATTCAGGTA AACTGGAAGA GTTTGTTCGA GGGAACCTTG AGAGAGAATG TATAGAAGAA 
AAGTGCAGTT TTGAAGAAGC ACGGGAAGTT TTTGAAAACA CTGAAAAAAC CACTGAATTT 
TGGAAGCAAT ATGTTGATGG AGATCAATGT GAATCCAATC CATGTTTAAA TGACGGTGTA 
TGCAAGGATG ACATTAATTC CTATGAATGT TGGTGTCGAG CTGGATTTGA AGGAAAGAAC 
TGTGAATTAG ATGTAACATG CAACATTAAG AATGGCAGAT GCAAGCAGTT TTGTAAATTG 
GGCCCCGATA ACAAGGTGGT TTGTTCCTGT ACTACGGGAT ACCAACTTGC GGAAGACCAA 
AGGTCCTGTG AACCAGCAGT GCCATTTCCA TGTGGAAGAG TTTCTGTCCC TCACATTTCT 
ATGACACGCA CCCGTGCTGA AACTCTTTTT TCCAATATGG ACTATGAAAA TTCAACTGAA 
GTGGAAAAAA TTTTGGATAA CGTCACCCAA CCGCTTAACG ACTTCACTCG AGTTGTTGGT 
GGAAAAGATG CCAAACCAGG TCAATTCCCT TGGCAGGTCC TTTTGAATGG GAAAGTTGAT 
GCATTCTGCG GAGGTTCCAT CATCAATGAA AAATGGGTGG TAACTGCAGC CCACTGTATT 
GAGCCTGATG TTAAAATTAC CATAGTTGCA GGTGAGCATA ACACCGAGAA GAGGGAACAT 
ACAGAGCAGA AGCGAAACGT GATTCGCACT ATTCTTCACC ACAGCTATAA TGCAACTATT 
AATAAGTACA ACCATGACAT CGCCCTTCTG GAACTGGATG AGCCCTTAAC GCTGAACAGC 
TATGTAACAC CTATTTGCAT TGCTGACAGG GAATACTCGA ACATCTTCCT CAAATTTGGG 
TCTGGCTATG TGAGTGGCTG GGGGAGAGTC TTCAACAAAG GGCGATCGGC TTCAATTCTT 
CAATACCTTA AAGTTCCACT TGTTGACCGA GCCACGTGCC TTCGGTCCAC GAAGTTCACC 
ATTTATAACA ACATGTTCTG TGCTGGCTTC CATGAGGGAG GTAAAGATTC ATGCCAGGGC 
GATAGTGGGG GACCCCATGT CACCGAAGTA GAAGGCATAA GTTTCTTAAC TGGGATTATT 
AGCTGGGGTG AAGAGTGTGC GATGAAAGGG AAGTATGGAA TATATACCAA GGTGTCCCGG 
TATGTCAACT GGATTAAAGA AAAGACGAAG CTCACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001003323.1
CDS	225..1583
Translation	MAEASGLVTVCLLGYLLSAECAVFLDRENATKILSRPKRYNSGKLEEFVRGNLERECIEEKCSFEEAREVFENTEKTTEFWKQYVDGDQCESNPCLNDGVCKDDINSYECWCRAGFEGKNCELDVTCNIKNGRCKQFCKLGPDNKVVCSCTTGYQLAEDQRSCEPAVPFPCGRVSVPHISMTRTRAETLFSNMDYENSTEVEKILDNVTQPLNDFTRVVGGKDAKPGQFPWQVLLNGKVDAFCGGSIINEKWVVTAAHCIEPDVKITIVAGEHNTEKREHTEQKRNVIRTILHHSYNATINKYNHDIALLELDEPLTLNSYVTPICIADREYSNIFLKFGSGYVSGWGRVFNKGRSASILQYLKVPLVDRATCLRSTKFTIYNNMFCAGFHEGGKDSCQGDSGGPHVTEVEGISFLTGIISWGEECAMKGKYGIYTKVSRYVNWIKEKTKLT

Target ORF information:

RefSeq Version	NM_001003323.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris coagulation factor IX (F9), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001003323.2

ORF Insert Sequence:

ATGGCAGAAG CATCGGGCCT CGTCACCGTC TGCCTTTTAG GATATCTACT CAGTGCCGAA 
TGTGCAGTTT TTCTTGATCG TGAAAATGCC ACCAAAATTC TGAGTCGGCC AAAGAGGTAT 
AATTCAGGTA AACTGGAAGA GTTTGTTCGA GGGAACCTTG AGAGAGAATG TATAGAAGAA 
AAGTGCAGTT TTGAAGAAGC ACGGGAAGTT TTTGAAAACA CTGAAAAAAC CACTGAATTT 
TGGAAGCAAT ATGTTGATGG AGATCAATGT GAATCCAATC CATGTTTAAA TGACGGTGTA 
TGCAAGGATG ACATTAATTC CTATGAATGT TGGTGTCGAG CTGGATTTGA AGGAAAGAAC 
TGTGAATTAG ATGTAACATG CAACATTAAG AATGGCAGAT GCAAGCAGTT TTGTAAATTG 
GGCCCCGATA ACAAGGTGGT TTGTTCCTGT ACTACGGGAT ACCAACTTGC GGAAGACCAA 
AGGTCCTGTG AACCAGCAGT GCCATTTCCA TGTGGAAGAG TTTCTGTCCC TCACATTTCT 
ATGACACGCA CCCGTGCTGA AACTCTTTTT TCCAATATGG ACTATGAAAA TTCAACTGAA 
GTGGAAAAAA TTTTGGATAA CGTCACCCAA CCGCTTAACG ACTTCACTCG AGTTGTTGGT 
GGAAAAGATG CCAAACCAGG TCAATTCCCT TGGCAGGTCC TTTTGAATGG GAAAGTTGAT 
GCATTCTGCG GAGGTTCCAT CATCAATGAA AAATGGGTGG TAACTGCAGC CCACTGTATT 
GAGCCTGATG TTAAAATTAC CATAGTTGCA GGTGAGCATA ACACCGAGAA GAGGGAACAT 
ACAGAGCAGA AGCGAAACGT GATTCGCACT ATTCTTCACC ACAGCTATAA TGCAACTATT 
AATAAGTACA ACCATGACAT CGCCCTTCTG GAACTGGATG AGCCCTTAAC GCTGAACAGC 
TATGTAACAC CTATTTGCAT TGCTGACAGG GAATACTCGA ACATCTTCCT CAAATTTGGG 
TCTGGCTATG TGAGTGGCTG GGGGAGAGTC TTCAACAAAG GGCGATCGGC TTCAATTCTT 
CAATACCTTA AAGTTCCACT TGTTGACCGA GCCACGTGCC TTCGGTCCAC GAAGTTCACC 
ATTTATAACA ACATGTTCTG TGCTGGCTTC CATGAGGGAG GTAAAGATTC ATGCCAGGGC 
GATAGTGGGG GACCCCATGT CACCGAAGTA GAAGGCATAA GTTTCTTAAC TGGGATTATT 
AGCTGGGGTG AAGAGTGTGC GATGAAAGGG AAGTATGGAA TATATACCAA GGTGTCCCGG 
TATGTCAACT GGATTAAAGA AAAGACGAAG CTCACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The efficacy and the risk of immunogenicity of FIX Padua (R338L) in hemophilia B dogs treated by AAV muscle gene therapy.
Blood120(23)4521-3(2012 Nov)
Finn JD,Nichols TC,Svoronos N,Merricks EP,Bellenger DA,Zhou S,Simioni P,High KA,Arruda VR

Hyperfunctional coagulation factor IX improves the efficacy of gene therapy in hemophilic mice.
Blood120(23)4517-20(2012 Nov)
Cantore A,Nair N,Della Valle P,Di Matteo M,M?trai J,Sanvito F,Brombin C,Di Serio C,D'Angelo A,Chuah M,Naldini L,Vandendriessche T

Sensitivity of whole blood clotting time and activated partial thromboplastin time for factor IX: relevance to gene therapy and determination of post-transfusion elimination time of canine factor IX in hemophilia B dogs.
Journal of thrombosis and haemostasis : JTH10(3)474-6(2012 Mar)
Nichols TC,Franck HW,Franck CT,De Friess N,Raymer RA,Merricks EP

Successful in vivo propagation of factor IX-producing hepatocytes in mice: potential for cell-based therapy in haemophilia B.
Thrombosis and haemostasis99(5)883-91(2008 May)
Tatsumi K,Ohashi K,Kataoka M,Tateno C,Shibata M,Naka H,Shima M,Hisanaga M,Kanehiro H,Okano T,Yoshizato K,Nakajima Y,Yoshioka A

Molecular cloning of a cDNA encoding canine factor IX.
Blood74(1)207-12(1989 Jul)
Evans JP,Watzke HH,Ware JL,Stafford DW,High KA