DGKG cDNA ORF clone, Calidris pugnax(ruff)

Gene
Clones
gRNAs

Summary

Gene Symbol	DGKG
Entrez Gene ID	106900806
Full Name	diacylglycerol kinase gamma
Gene Type	protein-coding
Organism	Calidris pugnax(ruff)

mRNA and Protein(s)

mRNA	Protein	Name
XM_014962954.1	XP_014818440.1	diacylglycerol kinase gamma isoform X1
XM_014962955.1	XP_014818441.1	diacylglycerol kinase gamma isoform X2

Pathways from BioSystems
Gene Ontology

The following DGKG gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DGKG cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCa80126	XM_014962954.1 Latest version!	Calidris pugnax diacylglycerol kinase gamma (DGKG), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OCa80127	XM_014962955.1 Latest version!	Calidris pugnax diacylglycerol kinase gamma (DGKG), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DGKG ( XM_014962954.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014818440.1 Calidris pugnax diacylglycerol kinase gamma (DGKG), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCa80126
Clone ID Related Accession (Same CDS sequence)	XM_014962954.1
Accession Version	XM_014962954.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2853bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	diacylglycerol kinase gamma isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015094503.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCCCT TTCCTCTGGG GCTGATTCAG GAGGAAGGTG CTGACCGGCT GCTCTGGCGC 
TGGGGTTTGC TGCCCACGGG CTCAGCGGGA TGGTGCCAGC CAGCCAAGAG CCCCCCGTGC 
CCCTCACGGT GCCAATGTGA CCACGCTGGC TGGGACCTGC GGCACCGAGC CTGGCACGGG 
GAGCACAGTG ACTCGTGGTC CCCCTTTGGG GTGCACCGCC CCAAGATGAG CGATGGTGGG 
TGGATCTCGC TCAGCCCCAC TGAGTTTGCC CAGCTCCAGC AGTACACAGA CTATTCCACC 
AAGAAGCTCA AGGATGTCCT TGAGGAGTTC CACGGGGACG GTGTCCTCTC GAGGTACAAC 
CCGGAACAGC CCATCGACTA TGAGGGCTTT CGCCTCTTCA TGAAGACCTA CCTGGAGGCG 
GACGTGCCCG AGGAACTTTG CCAACACCTC TTCACCTCCT TCAAGCGTAA GATATGCCAA 
GCCTCCCCCG AGAGCCAGCG CCAGGGCCCC AGCGTCTCGC AGCTCAACAC CCTCGGGATC 
AACGGGAAAA TGCTCCTAAG TGCCCTGGGA CGACACACCC CTGAGCCCAA GAGCTGTCAC 
AGCAACGCAG CTGAGCCGCA GCCAGCGTCC CTTACCCCGG CGTCGATCCC CAACGCCTCC 
CCAAGCTGGT CCAGGTCCTC CTCCCAGAAG TCCACCACCG GCACCCCTTC TGCTCACAAC 
TCCTCGGGCT CTTCGAAGGT CTCCTCCGGG TCCCTGCTCA GCCCTCAGTC GCCAGGCACA 
CGGAGTGTGG AGAAGAGGTT GCCCTTCAGC CTGCGGAAGA GCCACAGCTC CCTGAAAGCC 
GCCCCGCCAC TGCCCACCGC CCCCCTGTCC CAGGTGGTCC ATCTGAAGGA CATCGTCTGC 
TACCTGTCCC TGCTGGAGAG GGGACGGGCA GAGGACAAGC TGGAGTTTAT GTTTCGCCTC 
TATGATACTG ATGGAAATGG CCTCCTGGAC AGCTCGGAGC TGGATCGTAT CATCAACCAG 
ATGGTGCATG TGGCTGAGTA CCTGGAGTGG GACTCCACCG AGCTGAAGCC TATCTTGAAG 
GCGATGATGG AGGAGATCGA CTATGACCAT GACGGGACCG TGACGCTTGA GGAGTGGATC 
CGGGGTGGCA TGACCACCAT CCCCTTACTG GTCCTCCTGG GGATGGAGAC AAACGTGAAG 
GACGACGGGC AGCACGCCTG GAGGCTGAAG CACTTCAAGA AACCTGCCTA CTGCAACTTC 
TGCCGCACCA TGCTGCTGGG GGTCCGCAAG CAAGGACTCT GCTGCTCCTT CTGCAAGTAC 
ACTGTCCACG AGAGGTGTGT GTCCAAAGAT ATCGCCTCCT GCATCAGCAC CTACATGAAA 
TCCAAGAGGA ACACAAGCGT GATGCAGCAC ACCTGGATTG AGGGCAATTC CCCCGTCAAG 
TGCGACAGAT GCCACAAGAG CATCAAGTGC TACCAGGGCA TCACCGGCCT GCACTGCGTC 
TGGTGCCAAG TCACACTCCA CAACAAATGT GCCTCCCACG TGAAGCCAGA GTGCGACGGG 
GGCCCGCTGC GGGACCACAT CTTGCTGCCT TCCTACATCT GCCCCGTCGT CCTGGACAGG 
CAGTCCCACT GCCGGAGGTC AGAGAGTGAC TCCCCTGCCA GCACCAGCCC CGAGGACAAC 
CAGGGCTTCA AGTTCAACTC CACCACGGTG GATGGGCAAG GTCTGCAGAT CAGCCCCCGG 
CCTGGCACAC ACCCCCTCCT GGTGCTCGTG AACCCCAAGA GCGGAGGAAG GCAAGGGGAG 
CGGGTCCTCC GGAAATTTCA CTACTTGCTC AACCCACGCC AAGTGTACAA CCTGGACCGT 
GGTGGGCCCG CACCAGGGCT GAGCTTCTTT CGAGACACTC CGGATTTCCG TGTCCTGGCC 
TGTGGGGGGG ATGGCACAGT GGGCTGGATC CTGGACTGCA TCGACAAAGC GAACTTGGTG 
AAGCACCCGC CGGTGGCCGT CCTGCCCCTG GGAACAGGCA ACGACCTGGC CCGGTGCCTG 
CGCTGGGGAG GAGGCTATGA AGGAGGAAAC CTGATGAAGG TCCTGAAAGA CATCGAGCAC 
AGCACGGAGG TGATGCTGGA CCGCTGGCAG ATAGATGTCA TTCCCAGTGA CAGAGAGGCC 
AACGGAGACC CTGTCCCATC CACCATCATC AACAACTACT TCTCCATTGG GGTGGACGCC 
TCCATCGCCC ACCGCTTCCA CGTCATGCGA GAAAAGCACC CCGAGAAATT CAACAGCAGG 
ATGAAGAACA AGCTGTGGTA CTTTGAGTTT GGGACATCGG AGACCTTTGC GGCCACCTGC 
AAGAAGCTCC ACGACTACGT TGAGGTGGAG TGCGACGGGA CCCTGCTGGA CCTGAGCAAC 
ACGTCCCTGG AGGGCATCGC CGTCCTCAAC ATCCCCAGCA TGTACGGCGG CTCCAACCTC 
TGGGGCGAGA CCAAGAAGCA GCGCAGTTAC AGCCGGCTGG GGAAGAAAGC CCCGGAGAAG 
CTGCAGGGCA CGGTGGTCAC CGATGCCAAG GAGCTCAAGT TCTGCGTGCA GGATCTCAGT 
GACCACCTCC TGGAGGTGGT GGGGCTGGAG GGCGCGATGG AGATGGGGCA GATCTACACG 
GGGCTGAAGA GCGCTGGGAA GAGACTGGCC CAGTGTTCAT CTGTCACCAT CAGGACCTCC 
AAGCTGCTGC CCATGCAGGT GGACGGGGAA CCCTGGATGC AGCCGTCCTG TACCGTCAAA 
ATTACACATA AAAGCCAAGT GCCGATGCTG CTGGGCCCCC CCCAGAAGAG CAGCTTCTTT 
TTCCTGAAGA GGAGAAACCG AACTCGAGAT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014818440.1
CDS	69..2921
Translation	MLPFPLGLIQEEGADRLLWRWGLLPTGSAGWCQPAKSPPCPSRCQCDHAGWDLRHRAWHGEHSDSWSPFGVHRPKMSDGGWISLSPTEFAQLQQYTDYSTKKLKDVLEEFHGDGVLSRYNPEQPIDYEGFRLFMKTYLEADVPEELCQHLFTSFKRKICQASPESQRQGPSVSQLNTLGINGKMLLSALGRHTPEPKSCHSNAAEPQPASLTPASIPNASPSWSRSSSQKSTTGTPSAHNSSGSSKVSSGSLLSPQSPGTRSVEKRLPFSLRKSHSSLKAAPPLPTAPLSQVVHLKDIVCYLSLLERGRAEDKLEFMFRLYDTDGNGLLDSSELDRIINQMVHVAEYLEWDSTELKPILKAMMEEIDYDHDGTVTLEEWIRGGMTTIPLLVLLGMETNVKDDGQHAWRLKHFKKPAYCNFCRTMLLGVRKQGLCCSFCKYTVHERCVSKDIASCISTYMKSKRNTSVMQHTWIEGNSPVKCDRCHKSIKCYQGITGLHCVWCQVTLHNKCASHVKPECDGGPLRDHILLPSYICPVVLDRQSHCRRSESDSPASTSPEDNQGFKFNSTTVDGQGLQISPRPGTHPLLVLVNPKSGGRQGERVLRKFHYLLNPRQVYNLDRGGPAPGLSFFRDTPDFRVLACGGDGTVGWILDCIDKANLVKHPPVAVLPLGTGNDLARCLRWGGGYEGGNLMKVLKDIEHSTEVMLDRWQIDVIPSDREANGDPVPSTIINNYFSIGVDASIAHRFHVMREKHPEKFNSRMKNKLWYFEFGTSETFAATCKKLHDYVEVECDGTLLDLSNTSLEGIAVLNIPSMYGGSNLWGETKKQRSYSRLGKKAPEKLQGTVVTDAKELKFCVQDLSDHLLEVVGLEGAMEMGQIYTGLKSAGKRLAQCSSVTIRTSKLLPMQVDGEPWMQPSCTVKITHKSQVPMLLGPPQKSSFFFLKRRNRTRD

Target ORF information:

RefSeq Version	XM_014962954.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax diacylglycerol kinase gamma (DGKG), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014962954.1

ORF Insert Sequence:

ATGCTGCCCT TTCCTCTGGG GCTGATTCAG GAGGAAGGTG CTGACCGGCT GCTCTGGCGC 
TGGGGTTTGC TGCCCACGGG CTCAGCGGGA TGGTGCCAGC CAGCCAAGAG CCCCCCGTGC 
CCCTCACGGT GCCAATGTGA CCACGCTGGC TGGGACCTGC GGCACCGAGC CTGGCACGGG 
GAGCACAGTG ACTCGTGGTC CCCCTTTGGG GTGCACCGCC CCAAGATGAG CGATGGTGGG 
TGGATCTCGC TCAGCCCCAC TGAGTTTGCC CAGCTCCAGC AGTACACAGA CTATTCCACC 
AAGAAGCTCA AGGATGTCCT TGAGGAGTTC CACGGGGACG GTGTCCTCTC GAGGTACAAC 
CCGGAACAGC CCATCGACTA TGAGGGCTTT CGCCTCTTCA TGAAGACCTA CCTGGAGGCG 
GACGTGCCCG AGGAACTTTG CCAACACCTC TTCACCTCCT TCAAGCGTAA GATATGCCAA 
GCCTCCCCCG AGAGCCAGCG CCAGGGCCCC AGCGTCTCGC AGCTCAACAC CCTCGGGATC 
AACGGGAAAA TGCTCCTAAG TGCCCTGGGA CGACACACCC CTGAGCCCAA GAGCTGTCAC 
AGCAACGCAG CTGAGCCGCA GCCAGCGTCC CTTACCCCGG CGTCGATCCC CAACGCCTCC 
CCAAGCTGGT CCAGGTCCTC CTCCCAGAAG TCCACCACCG GCACCCCTTC TGCTCACAAC 
TCCTCGGGCT CTTCGAAGGT CTCCTCCGGG TCCCTGCTCA GCCCTCAGTC GCCAGGCACA 
CGGAGTGTGG AGAAGAGGTT GCCCTTCAGC CTGCGGAAGA GCCACAGCTC CCTGAAAGCC 
GCCCCGCCAC TGCCCACCGC CCCCCTGTCC CAGGTGGTCC ATCTGAAGGA CATCGTCTGC 
TACCTGTCCC TGCTGGAGAG GGGACGGGCA GAGGACAAGC TGGAGTTTAT GTTTCGCCTC 
TATGATACTG ATGGAAATGG CCTCCTGGAC AGCTCGGAGC TGGATCGTAT CATCAACCAG 
ATGGTGCATG TGGCTGAGTA CCTGGAGTGG GACTCCACCG AGCTGAAGCC TATCTTGAAG 
GCGATGATGG AGGAGATCGA CTATGACCAT GACGGGACCG TGACGCTTGA GGAGTGGATC 
CGGGGTGGCA TGACCACCAT CCCCTTACTG GTCCTCCTGG GGATGGAGAC AAACGTGAAG 
GACGACGGGC AGCACGCCTG GAGGCTGAAG CACTTCAAGA AACCTGCCTA CTGCAACTTC 
TGCCGCACCA TGCTGCTGGG GGTCCGCAAG CAAGGACTCT GCTGCTCCTT CTGCAAGTAC 
ACTGTCCACG AGAGGTGTGT GTCCAAAGAT ATCGCCTCCT GCATCAGCAC CTACATGAAA 
TCCAAGAGGA ACACAAGCGT GATGCAGCAC ACCTGGATTG AGGGCAATTC CCCCGTCAAG 
TGCGACAGAT GCCACAAGAG CATCAAGTGC TACCAGGGCA TCACCGGCCT GCACTGCGTC 
TGGTGCCAAG TCACACTCCA CAACAAATGT GCCTCCCACG TGAAGCCAGA GTGCGACGGG 
GGCCCGCTGC GGGACCACAT CTTGCTGCCT TCCTACATCT GCCCCGTCGT CCTGGACAGG 
CAGTCCCACT GCCGGAGGTC AGAGAGTGAC TCCCCTGCCA GCACCAGCCC CGAGGACAAC 
CAGGGCTTCA AGTTCAACTC CACCACGGTG GATGGGCAAG GTCTGCAGAT CAGCCCCCGG 
CCTGGCACAC ACCCCCTCCT GGTGCTCGTG AACCCCAAGA GCGGAGGAAG GCAAGGGGAG 
CGGGTCCTCC GGAAATTTCA CTACTTGCTC AACCCACGCC AAGTGTACAA CCTGGACCGT 
GGTGGGCCCG CACCAGGGCT GAGCTTCTTT CGAGACACTC CGGATTTCCG TGTCCTGGCC 
TGTGGGGGGG ATGGCACAGT GGGCTGGATC CTGGACTGCA TCGACAAAGC GAACTTGGTG 
AAGCACCCGC CGGTGGCCGT CCTGCCCCTG GGAACAGGCA ACGACCTGGC CCGGTGCCTG 
CGCTGGGGAG GAGGCTATGA AGGAGGAAAC CTGATGAAGG TCCTGAAAGA CATCGAGCAC 
AGCACGGAGG TGATGCTGGA CCGCTGGCAG ATAGATGTCA TTCCCAGTGA CAGAGAGGCC 
AACGGAGACC CTGTCCCATC CACCATCATC AACAACTACT TCTCCATTGG GGTGGACGCC 
TCCATCGCCC ACCGCTTCCA CGTCATGCGA GAAAAGCACC CCGAGAAATT CAACAGCAGG 
ATGAAGAACA AGCTGTGGTA CTTTGAGTTT GGGACATCGG AGACCTTTGC GGCCACCTGC 
AAGAAGCTCC ACGACTACGT TGAGGTGGAG TGCGACGGGA CCCTGCTGGA CCTGAGCAAC 
ACGTCCCTGG AGGGCATCGC CGTCCTCAAC ATCCCCAGCA TGTACGGCGG CTCCAACCTC 
TGGGGCGAGA CCAAGAAGCA GCGCAGTTAC AGCCGGCTGG GGAAGAAAGC CCCGGAGAAG 
CTGCAGGGCA CGGTGGTCAC CGATGCCAAG GAGCTCAAGT TCTGCGTGCA GGATCTCAGT 
GACCACCTCC TGGAGGTGGT GGGGCTGGAG GGCGCGATGG AGATGGGGCA GATCTACACG 
GGGCTGAAGA GCGCTGGGAA GAGACTGGCC CAGTGTTCAT CTGTCACCAT CAGGACCTCC 
AAGCTGCTGC CCATGCAGGT GGACGGGGAA CCCTGGATGC AGCCGTCCTG TACCGTCAAA 
ATTACACATA AAAGCCAAGT GCCGATGCTG CTGGGCCCCC CCCAGAAGAG CAGCTTCTTT 
TTCCTGAAGA GGAGAAACCG AACTCGAGAT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DGKG ( XM_014962955.1 ) cDNA ORF clone, Calidris pugnax(ruff) -> XP_014818441.1 Calidris pugnax diacylglycerol kinase gamma (DGKG), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCa80127
Clone ID Related Accession (Same CDS sequence)	XM_014962955.1
Accession Version	XM_014962955.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2628bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-08
Organism	Calidris pugnax(ruff)
Product	diacylglycerol kinase gamma isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015094503.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Calidris pugnax Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGATG GTGGGTGGAT CTCGCTCAGC CCCACTGAGT TTGCCCAGCT CCAGCAGTAC 
ACAGACTATT CCACCAAGAA GCTCAAGGAT GTCCTTGAGG AGTTCCACGG GGACGGTGTC 
CTCTCGAGGT ACAACCCGGA ACAGCCCATC GACTATGAGG GCTTTCGCCT CTTCATGAAG 
ACCTACCTGG AGGCGGACGT GCCCGAGGAA CTTTGCCAAC ACCTCTTCAC CTCCTTCAAG 
CGTAAGATAT GCCAAGCCTC CCCCGAGAGC CAGCGCCAGG GCCCCAGCGT CTCGCAGCTC 
AACACCCTCG GGATCAACGG GAAAATGCTC CTAAGTGCCC TGGGACGACA CACCCCTGAG 
CCCAAGAGCT GTCACAGCAA CGCAGCTGAG CCGCAGCCAG CGTCCCTTAC CCCGGCGTCG 
ATCCCCAACG CCTCCCCAAG CTGGTCCAGG TCCTCCTCCC AGAAGTCCAC CACCGGCACC 
CCTTCTGCTC ACAACTCCTC GGGCTCTTCG AAGGTCTCCT CCGGGTCCCT GCTCAGCCCT 
CAGTCGCCAG GCACACGGAG TGTGGAGAAG AGGTTGCCCT TCAGCCTGCG GAAGAGCCAC 
AGCTCCCTGA AAGCCGCCCC GCCACTGCCC ACCGCCCCCC TGTCCCAGGT GGTCCATCTG 
AAGGACATCG TCTGCTACCT GTCCCTGCTG GAGAGGGGAC GGGCAGAGGA CAAGCTGGAG 
TTTATGTTTC GCCTCTATGA TACTGATGGA AATGGCCTCC TGGACAGCTC GGAGCTGGAT 
CGTATCATCA ACCAGATGGT GCATGTGGCT GAGTACCTGG AGTGGGACTC CACCGAGCTG 
AAGCCTATCT TGAAGGCGAT GATGGAGGAG ATCGACTATG ACCATGACGG GACCGTGACG 
CTTGAGGAGT GGATCCGGGG TGGCATGACC ACCATCCCCT TACTGGTCCT CCTGGGGATG 
GAGACAAACG TGAAGGACGA CGGGCAGCAC GCCTGGAGGC TGAAGCACTT CAAGAAACCT 
GCCTACTGCA ACTTCTGCCG CACCATGCTG CTGGGGGTCC GCAAGCAAGG ACTCTGCTGC 
TCCTTCTGCA AGTACACTGT CCACGAGAGG TGTGTGTCCA AAGATATCGC CTCCTGCATC 
AGCACCTACA TGAAATCCAA GAGGAACACA AGCGTGATGC AGCACACCTG GATTGAGGGC 
AATTCCCCCG TCAAGTGCGA CAGATGCCAC AAGAGCATCA AGTGCTACCA GGGCATCACC 
GGCCTGCACT GCGTCTGGTG CCAAGTCACA CTCCACAACA AATGTGCCTC CCACGTGAAG 
CCAGAGTGCG ACGGGGGCCC GCTGCGGGAC CACATCTTGC TGCCTTCCTA CATCTGCCCC 
GTCGTCCTGG ACAGGCAGTC CCACTGCCGG AGGTCAGAGA GTGACTCCCC TGCCAGCACC 
AGCCCCGAGG ACAACCAGGG CTTCAAGTTC AACTCCACCA CGGTGGATGG GCAAGGTCTG 
CAGATCAGCC CCCGGCCTGG CACACACCCC CTCCTGGTGC TCGTGAACCC CAAGAGCGGA 
GGAAGGCAAG GGGAGCGGGT CCTCCGGAAA TTTCACTACT TGCTCAACCC ACGCCAAGTG 
TACAACCTGG ACCGTGGTGG GCCCGCACCA GGGCTGAGCT TCTTTCGAGA CACTCCGGAT 
TTCCGTGTCC TGGCCTGTGG GGGGGATGGC ACAGTGGGCT GGATCCTGGA CTGCATCGAC 
AAAGCGAACT TGGTGAAGCA CCCGCCGGTG GCCGTCCTGC CCCTGGGAAC AGGCAACGAC 
CTGGCCCGGT GCCTGCGCTG GGGAGGAGGC TATGAAGGAG GAAACCTGAT GAAGGTCCTG 
AAAGACATCG AGCACAGCAC GGAGGTGATG CTGGACCGCT GGCAGATAGA TGTCATTCCC 
AGTGACAGAG AGGCCAACGG AGACCCTGTC CCATCCACCA TCATCAACAA CTACTTCTCC 
ATTGGGGTGG ACGCCTCCAT CGCCCACCGC TTCCACGTCA TGCGAGAAAA GCACCCCGAG 
AAATTCAACA GCAGGATGAA GAACAAGCTG TGGTACTTTG AGTTTGGGAC ATCGGAGACC 
TTTGCGGCCA CCTGCAAGAA GCTCCACGAC TACGTTGAGG TGGAGTGCGA CGGGACCCTG 
CTGGACCTGA GCAACACGTC CCTGGAGGGC ATCGCCGTCC TCAACATCCC CAGCATGTAC 
GGCGGCTCCA ACCTCTGGGG CGAGACCAAG AAGCAGCGCA GTTACAGCCG GCTGGGGAAG 
AAAGCCCCGG AGAAGCTGCA GGGCACGGTG GTCACCGATG CCAAGGAGCT CAAGTTCTGC 
GTGCAGGATC TCAGTGACCA CCTCCTGGAG GTGGTGGGGC TGGAGGGCGC GATGGAGATG 
GGGCAGATCT ACACGGGGCT GAAGAGCGCT GGGAAGAGAC TGGCCCAGTG TTCATCTGTC 
ACCATCAGGA CCTCCAAGCT GCTGCCCATG CAGGTGGACG GGGAACCCTG GATGCAGCCG 
TCCTGTACCG TCAAAATTAC ACATAAAAGC CAAGTGCCGA TGCTGCTGGG CCCCCCCCAG 
AAGAGCAGCT TCTTTTTCCT GAAGAGGAGA AACCGAACTC GAGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014818441.1
CDS	223..2850
Translation	MSDGGWISLSPTEFAQLQQYTDYSTKKLKDVLEEFHGDGVLSRYNPEQPIDYEGFRLFMKTYLEADVPEELCQHLFTSFKRKICQASPESQRQGPSVSQLNTLGINGKMLLSALGRHTPEPKSCHSNAAEPQPASLTPASIPNASPSWSRSSSQKSTTGTPSAHNSSGSSKVSSGSLLSPQSPGTRSVEKRLPFSLRKSHSSLKAAPPLPTAPLSQVVHLKDIVCYLSLLERGRAEDKLEFMFRLYDTDGNGLLDSSELDRIINQMVHVAEYLEWDSTELKPILKAMMEEIDYDHDGTVTLEEWIRGGMTTIPLLVLLGMETNVKDDGQHAWRLKHFKKPAYCNFCRTMLLGVRKQGLCCSFCKYTVHERCVSKDIASCISTYMKSKRNTSVMQHTWIEGNSPVKCDRCHKSIKCYQGITGLHCVWCQVTLHNKCASHVKPECDGGPLRDHILLPSYICPVVLDRQSHCRRSESDSPASTSPEDNQGFKFNSTTVDGQGLQISPRPGTHPLLVLVNPKSGGRQGERVLRKFHYLLNPRQVYNLDRGGPAPGLSFFRDTPDFRVLACGGDGTVGWILDCIDKANLVKHPPVAVLPLGTGNDLARCLRWGGGYEGGNLMKVLKDIEHSTEVMLDRWQIDVIPSDREANGDPVPSTIINNYFSIGVDASIAHRFHVMREKHPEKFNSRMKNKLWYFEFGTSETFAATCKKLHDYVEVECDGTLLDLSNTSLEGIAVLNIPSMYGGSNLWGETKKQRSYSRLGKKAPEKLQGTVVTDAKELKFCVQDLSDHLLEVVGLEGAMEMGQIYTGLKSAGKRLAQCSSVTIRTSKLLPMQVDGEPWMQPSCTVKITHKSQVPMLLGPPQKSSFFFLKRRNRTRD

Target ORF information:

RefSeq Version	XM_014962955.1
Organism	Calidris pugnax(ruff)
Definition	Calidris pugnax diacylglycerol kinase gamma (DGKG), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014962955.1

ORF Insert Sequence:

ATGAGCGATG GTGGGTGGAT CTCGCTCAGC CCCACTGAGT TTGCCCAGCT CCAGCAGTAC 
ACAGACTATT CCACCAAGAA GCTCAAGGAT GTCCTTGAGG AGTTCCACGG GGACGGTGTC 
CTCTCGAGGT ACAACCCGGA ACAGCCCATC GACTATGAGG GCTTTCGCCT CTTCATGAAG 
ACCTACCTGG AGGCGGACGT GCCCGAGGAA CTTTGCCAAC ACCTCTTCAC CTCCTTCAAG 
CGTAAGATAT GCCAAGCCTC CCCCGAGAGC CAGCGCCAGG GCCCCAGCGT CTCGCAGCTC 
AACACCCTCG GGATCAACGG GAAAATGCTC CTAAGTGCCC TGGGACGACA CACCCCTGAG 
CCCAAGAGCT GTCACAGCAA CGCAGCTGAG CCGCAGCCAG CGTCCCTTAC CCCGGCGTCG 
ATCCCCAACG CCTCCCCAAG CTGGTCCAGG TCCTCCTCCC AGAAGTCCAC CACCGGCACC 
CCTTCTGCTC ACAACTCCTC GGGCTCTTCG AAGGTCTCCT CCGGGTCCCT GCTCAGCCCT 
CAGTCGCCAG GCACACGGAG TGTGGAGAAG AGGTTGCCCT TCAGCCTGCG GAAGAGCCAC 
AGCTCCCTGA AAGCCGCCCC GCCACTGCCC ACCGCCCCCC TGTCCCAGGT GGTCCATCTG 
AAGGACATCG TCTGCTACCT GTCCCTGCTG GAGAGGGGAC GGGCAGAGGA CAAGCTGGAG 
TTTATGTTTC GCCTCTATGA TACTGATGGA AATGGCCTCC TGGACAGCTC GGAGCTGGAT 
CGTATCATCA ACCAGATGGT GCATGTGGCT GAGTACCTGG AGTGGGACTC CACCGAGCTG 
AAGCCTATCT TGAAGGCGAT GATGGAGGAG ATCGACTATG ACCATGACGG GACCGTGACG 
CTTGAGGAGT GGATCCGGGG TGGCATGACC ACCATCCCCT TACTGGTCCT CCTGGGGATG 
GAGACAAACG TGAAGGACGA CGGGCAGCAC GCCTGGAGGC TGAAGCACTT CAAGAAACCT 
GCCTACTGCA ACTTCTGCCG CACCATGCTG CTGGGGGTCC GCAAGCAAGG ACTCTGCTGC 
TCCTTCTGCA AGTACACTGT CCACGAGAGG TGTGTGTCCA AAGATATCGC CTCCTGCATC 
AGCACCTACA TGAAATCCAA GAGGAACACA AGCGTGATGC AGCACACCTG GATTGAGGGC 
AATTCCCCCG TCAAGTGCGA CAGATGCCAC AAGAGCATCA AGTGCTACCA GGGCATCACC 
GGCCTGCACT GCGTCTGGTG CCAAGTCACA CTCCACAACA AATGTGCCTC CCACGTGAAG 
CCAGAGTGCG ACGGGGGCCC GCTGCGGGAC CACATCTTGC TGCCTTCCTA CATCTGCCCC 
GTCGTCCTGG ACAGGCAGTC CCACTGCCGG AGGTCAGAGA GTGACTCCCC TGCCAGCACC 
AGCCCCGAGG ACAACCAGGG CTTCAAGTTC AACTCCACCA CGGTGGATGG GCAAGGTCTG 
CAGATCAGCC CCCGGCCTGG CACACACCCC CTCCTGGTGC TCGTGAACCC CAAGAGCGGA 
GGAAGGCAAG GGGAGCGGGT CCTCCGGAAA TTTCACTACT TGCTCAACCC ACGCCAAGTG 
TACAACCTGG ACCGTGGTGG GCCCGCACCA GGGCTGAGCT TCTTTCGAGA CACTCCGGAT 
TTCCGTGTCC TGGCCTGTGG GGGGGATGGC ACAGTGGGCT GGATCCTGGA CTGCATCGAC 
AAAGCGAACT TGGTGAAGCA CCCGCCGGTG GCCGTCCTGC CCCTGGGAAC AGGCAACGAC 
CTGGCCCGGT GCCTGCGCTG GGGAGGAGGC TATGAAGGAG GAAACCTGAT GAAGGTCCTG 
AAAGACATCG AGCACAGCAC GGAGGTGATG CTGGACCGCT GGCAGATAGA TGTCATTCCC 
AGTGACAGAG AGGCCAACGG AGACCCTGTC CCATCCACCA TCATCAACAA CTACTTCTCC 
ATTGGGGTGG ACGCCTCCAT CGCCCACCGC TTCCACGTCA TGCGAGAAAA GCACCCCGAG 
AAATTCAACA GCAGGATGAA GAACAAGCTG TGGTACTTTG AGTTTGGGAC ATCGGAGACC 
TTTGCGGCCA CCTGCAAGAA GCTCCACGAC TACGTTGAGG TGGAGTGCGA CGGGACCCTG 
CTGGACCTGA GCAACACGTC CCTGGAGGGC ATCGCCGTCC TCAACATCCC CAGCATGTAC 
GGCGGCTCCA ACCTCTGGGG CGAGACCAAG AAGCAGCGCA GTTACAGCCG GCTGGGGAAG 
AAAGCCCCGG AGAAGCTGCA GGGCACGGTG GTCACCGATG CCAAGGAGCT CAAGTTCTGC 
GTGCAGGATC TCAGTGACCA CCTCCTGGAG GTGGTGGGGC TGGAGGGCGC GATGGAGATG 
GGGCAGATCT ACACGGGGCT GAAGAGCGCT GGGAAGAGAC TGGCCCAGTG TTCATCTGTC 
ACCATCAGGA CCTCCAAGCT GCTGCCCATG CAGGTGGACG GGGAACCCTG GATGCAGCCG 
TCCTGTACCG TCAAAATTAC ACATAAAAGC CAAGTGCCGA TGCTGCTGGG CCCCCCCCAG 
AAGAGCAGCT TCTTTTTCCT GAAGAGGAGA AACCGAACTC GAGATTAA