TSC1 cDNA ORF clone, Buceros rhinoceros silvestris

Gene
Clones
gRNAs

Summary

Gene Symbol	TSC1
Entrez Gene ID	104493433
Full Name	TSC complex subunit 1
Gene Type	protein-coding
Organism	Buceros rhinoceros silvestris

mRNA and Protein(s)

mRNA	Protein	Name
XM_010136107.1	XP_010134409.1	hamartin

Pathways from BioSystems
Gene Ontology

The following TSC1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TSC1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBu37729	XM_010136107.1 Latest version!	Buceros rhinoceros silvestris tuberous sclerosis 1 (TSC1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TSC1 ( XM_010136107.1 ) cDNA ORF clone, Buceros rhinoceros silvestris -> XP_010134409.1 Buceros rhinoceros silvestris tuberous sclerosis 1 (TSC1), mRNA.

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CloneID	OBu37729
Clone ID Related Accession (Same CDS sequence)	XM_010136107.1
Accession Version	XM_010136107.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3471bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-11-05
Organism	Buceros rhinoceros silvestris
Product	hamartin
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_010411715.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Buceros rhinoceros silvestris Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCACAGC AAGGAAATGT TGGTGAGCTC CTCTCCATGC TGGATTCTCC AATTCTGGGT 
GTCCTGGAAG ATATAACAGC TGCGTTTAAA GATAATCTCA TTTGTGACCG GGGACCTGTG 
CTTGTGAACA ACCTGGTGGA CTATTACTTG GAAACCAATT CTCAGCAGGC ACTGCATATA 
CTGTCCACGC TGCAAGAGCC TCATGACAAG CATCTTCTGG ACAAAATTAA TGAATATATG 
GGCAAAGCTG CTACTCGTTT ACCCACTCTC TCGCTGCTGG GACATGTGAT AAGACGACAA 
CCATCTTGGA AACATAAGCT TTCTCAAGCA CCTCTCCTAC TTTCATTACT GAAATGTCTC 
AAGACTGACA CAGATGTAGT AGTACTCACC ACAGGTGTCC TAGTGCTAAT AACCATGTTG 
CCAATGATTC CTCAGTCTGG CAAACAGTAC CTTCATGATT TCTTTGGTAT CTTTGGGCGT 
CTCTCAGCCT GGTGCTTGCA GAATCCAGGT CACGTGGCAG AGATTTATCT TGTCCATCTT 
CATGCCAGCG TTTATGCTCT CTTTCATCGT CTTTATGGAA TGTATCCTTG CAATTTTGTC 
TCCTTTCTGC GTTCTCACTA CAGTATGAAG GAAAACTTGG AGACCTTTGA AGAGGTAGTC 
AAGCCAATGA TGGAGCATGT GCGAATTCAT CCAGAATTAG TGACTGGATC TAAGGACCAT 
GAACTGGACC CACGAAGGTG GAAAAGACTA GAAACTCATG ATGTTGTGAT AGAATGTGCC 
AAAATCTCCC TGGACCCTGC AGAAGCCTCA TATGAAGATG GCTATTACTC TGTGTCTCGG 
AAACTCTGCA CAAGCTTAAA ACATCATCAA ACTGACCCCA GTGCCAGCTA TTACATTGAC 
ACACAGAGCA ACTATGGGAC TTCTACCCCA TATTCCACTC CTCGGCTAAC ACTATCACAA 
ATGCCAGGGC AGCTACCTCA GATTCTGAGC CCACAGTCAA TACGGCTGTC GACTGAGCCA 
CAGCAGGTTA CCATCTGGAG TCCTTCTGCA GTTTGTGGTA TGACCACTCC ACCAACCTCC 
CCTGGAATCA TCCCATCGGA GTCATCCCAA TCTGCATCAC AACCTTACAA CAAAGCTTTT 
GGCCCATCTG GAGGGGGAAA AGGAACACCT TTGGGAACGC CTGCCACATC TCCTCCACCC 
TGCACTTCTG ATGACTTTGT GCATGTTTCG CTCCCTTCAG CTGCTGCCAC GCCTCCAAAA 
AAGGAGGACA AACCAGATCC TGGGAGGCCT TTACTGTACC GACAGCAAAA TGTCATAAAC 
AGCGATAAAT CATTGGACAC ACCTGGTAGT AAAAGTTCAG TAACCTTAAG TGATCTTCCA 
GAGTTTTTAG ATCGTTTGTC TTTTGAAGAT AGTGCTGAAA AGGACAGAGA GGAAGATGCA 
ATATCTAAAG AGATCTCCGA GATCACAACT GATGCTGAAC ACATGGTGCC TAGAGGAGGA 
TTTGACTCTC CGTTTTACCG CACAAATGAA AGTCTGTCAG GCTCTCAAAA GAAGACCCAT 
TCAGTAGTCT CTAGTGTTCA GGGACACAGT CAGACCTCTG AGCCTTTAAC ATCTTCTCTG 
GACAAGCCTG GGCCTGAGAG TGCGCAGGAA ACACCCAAAC AAACGTTTAC TCCCATAGAC 
AAGCCCTGCG GAGGCTCTGG TGAAAGCCCT GCTGGTAACA GGGAAGGAAC CTCTGGGGAG 
ATGAGTATTC TCACTCCCAG CCCTTGCAAA ATACCAGCAC AGAGAAGAGC AGTGTTTGGG 
AGCGGGCAGC CTCCACTGTA CGACCACCTT TTTGAGGTTG CGTTACCAAA GACTGCCTAC 
TTCTTTGTTG GCAAGAAGAC TGAGGAGCTG CTGAAGAAAG TCAAGGGAAA CCAGGACGAT 
GACTGCATGT GCTCTACTTC TCCCGTGGAA GTGCTGGACA GACTGATACA GCAAGGAGCT 
GATGCCCACA CTAAGGAGCT GAACAAATTG TCTCTGCCAA GCAAATCTGC TGACTGGACT 
CACTTCGGAG GTTCTCCCCC TTCAGATGAG ATTCACACCC TACGTAACCA ATTACTTTTG 
CTGCACAACC AGTTACTGTA TGAACGCTTC AAAAGACAGC AACATGCCCT ACGGAACCGG 
CGACTCTTGC GAAAAGTGAT TAAAGCAACA GCTCTGGAGG AACATAATGC TGCCATGAAA 
GATCAGCTGA AACTACAGGA AAAAGAAATC CAGGCCTTGA AACTGAGTCT GCAGAAAGAA 
CAGGCAAGAT ACCACCAGTT TCAGGAGGAG CATGAAAATA TAGTGGCTCA GCTTCACAGC 
CAGATCAGGC AGCTGCAACA TGACCGGGAG GAATTCTACA ACCAGAGCCA GGAACTGCAG 
ACCAAGCTGG AAGACTGCCG GAATATGATT GCAGATCTGA GGTTAGAGTT AAAGAAGGCT 
AACAATAAAG TGTGTCACAC TGAATTGCTT CTTAGCCAAG TTTCTCAAAA GCTTTCCAAC 
AGTGAATCGG TGCAACAGCA GATGGAGTTC TTGAACAGGC AGCTTCTGGT TCTTGGAGAG 
GTCAATGAGT TGTACCTGGA GCAGCTGCAG CACAAGCACA CAGACACTAC AAAGGAGGTT 
GAAATGTTGC ATGCTGCTTT TCGGAAAGAA CTGGAGAAAG CAAAATTGTG TGTTCAACAG 
CAAAGCCAAA AGCTTGATGC TTCTCAGAAA CGGATAGCTG AACTGGAATC TCAGCTTGCT 
AAAAAGGACC ACCTCTTTCT GGAGCAAAAG AAGTACCTGG AAGATGTCAA AATCCAAGCA 
AGAGGTCAGC TGCAAGCTGT AGAAAGTAGG TACAAGGCCC AGAAAAGAAT CACACAGGCA 
TTTGAGCTGG AGATCTTGGA TCTGTTTGGC AGGCTGGAGA AGAGCAGCCT ACTGAAAAAA 
CTTGAAGATG ATAAAACAGA AGCAGCTGAA GCAGCAGAAG AAAGGTTGGA TGGTGGTAAT 
GAAGATACTG TGGGAGGAAA CAGTGAAGAA ACAATTGGGA GAAATGGAGA GACCAAACCT 
CCCAGCACTC GAGGTAGCAG TAGCAGTAAG GGTGGCAGCA GCAGTGAGCT CTCCACCCCA 
GAAAAACCCC AGAACCAGAG ATTCAGCAGT CGCTGGGAGA CATCTGTGTT GCTGGAGCCC 
TCGACTACTG TGCCACTGAC TGTAGGTTCA CTTCCCAGCT CCAAGAGCTT CCTTGGAATG 
AAGGCACGAG AATTATTTCG CAACAAGAGT GAGAGCCAGT GTGATGAGGA GGGTGTAACC 
ATCAACAGGC TTTCTGATGC TCTAAAGACT GATCTATGTA AAGACCCAAG CCTGGAGATG 
AAGGCCCCTC CAAGCCCCGA TAACCTTAGC CTCTCGCCTA AGATCCAGGA AAGCAGTGTT 
GGACAGCTTC ATATCATGGA CTACAATGAA ACTCATCATG ACCACAGTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010134409.1
CDS	1..3471
Translation	MAQQGNVGELLSMLDSPILGVLEDITAAFKDNLICDRGPVLVNNLVDYYLETNSQQALHILSTLQEPHDKHLLDKINEYMGKAATRLPTLSLLGHVIRRQPSWKHKLSQAPLLLSLLKCLKTDTDVVVLTTGVLVLITMLPMIPQSGKQYLHDFFGIFGRLSAWCLQNPGHVAEIYLVHLHASVYALFHRLYGMYPCNFVSFLRSHYSMKENLETFEEVVKPMMEHVRIHPELVTGSKDHELDPRRWKRLETHDVVIECAKISLDPAEASYEDGYYSVSRKLCTSLKHHQTDPSASYYIDTQSNYGTSTPYSTPRLTLSQMPGQLPQILSPQSIRLSTEPQQVTIWSPSAVCGMTTPPTSPGIIPSESSQSASQPYNKAFGPSGGGKGTPLGTPATSPPPCTSDDFVHVSLPSAAATPPKKEDKPDPGRPLLYRQQNVINSDKSLDTPGSKSSVTLSDLPEFLDRLSFEDSAEKDREEDAISKEISEITTDAEHMVPRGGFDSPFYRTNESLSGSQKKTHSVVSSVQGHSQTSEPLTSSLDKPGPESAQETPKQTFTPIDKPCGGSGESPAGNREGTSGEMSILTPSPCKIPAQRRAVFGSGQPPLYDHLFEVALPKTAYFFVGKKTEELLKKVKGNQDDDCMCSTSPVEVLDRLIQQGADAHTKELNKLSLPSKSADWTHFGGSPPSDEIHTLRNQLLLLHNQLLYERFKRQQHALRNRRLLRKVIKATALEEHNAAMKDQLKLQEKEIQALKLSLQKEQARYHQFQEEHENIVAQLHSQIRQLQHDREEFYNQSQELQTKLEDCRNMIADLRLELKKANNKVCHTELLLSQVSQKLSNSESVQQQMEFLNRQLLVLGEVNELYLEQLQHKHTDTTKEVEMLHAAFRKELEKAKLCVQQQSQKLDASQKRIAELESQLAKKDHLFLEQKKYLEDVKIQARGQLQAVESRYKAQKRITQAFELEILDLFGRLEKSSLLKKLEDDKTEAAEAAEERLDGGNEDTVGGNSEETIGRNGETKPPSTRGSSSSKGGSSSELSTPEKPQNQRFSSRWETSVLLEPSTTVPLTVGSLPSSKSFLGMKARELFRNKSESQCDEEGVTINRLSDALKTDLCKDPSLEMKAPPSPDNLSLSPKIQESSVGQLHIMDYNETHHDHS

Target ORF information:

RefSeq Version	XM_010136107.1
Organism	Buceros rhinoceros silvestris
Definition	Buceros rhinoceros silvestris tuberous sclerosis 1 (TSC1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010136107.1

ORF Insert Sequence:

ATGGCACAGC AAGGAAATGT TGGTGAGCTC CTCTCCATGC TGGATTCTCC AATTCTGGGT 
GTCCTGGAAG ATATAACAGC TGCGTTTAAA GATAATCTCA TTTGTGACCG GGGACCTGTG 
CTTGTGAACA ACCTGGTGGA CTATTACTTG GAAACCAATT CTCAGCAGGC ACTGCATATA 
CTGTCCACGC TGCAAGAGCC TCATGACAAG CATCTTCTGG ACAAAATTAA TGAATATATG 
GGCAAAGCTG CTACTCGTTT ACCCACTCTC TCGCTGCTGG GACATGTGAT AAGACGACAA 
CCATCTTGGA AACATAAGCT TTCTCAAGCA CCTCTCCTAC TTTCATTACT GAAATGTCTC 
AAGACTGACA CAGATGTAGT AGTACTCACC ACAGGTGTCC TAGTGCTAAT AACCATGTTG 
CCAATGATTC CTCAGTCTGG CAAACAGTAC CTTCATGATT TCTTTGGTAT CTTTGGGCGT 
CTCTCAGCCT GGTGCTTGCA GAATCCAGGT CACGTGGCAG AGATTTATCT TGTCCATCTT 
CATGCCAGCG TTTATGCTCT CTTTCATCGT CTTTATGGAA TGTATCCTTG CAATTTTGTC 
TCCTTTCTGC GTTCTCACTA CAGTATGAAG GAAAACTTGG AGACCTTTGA AGAGGTAGTC 
AAGCCAATGA TGGAGCATGT GCGAATTCAT CCAGAATTAG TGACTGGATC TAAGGACCAT 
GAACTGGACC CACGAAGGTG GAAAAGACTA GAAACTCATG ATGTTGTGAT AGAATGTGCC 
AAAATCTCCC TGGACCCTGC AGAAGCCTCA TATGAAGATG GCTATTACTC TGTGTCTCGG 
AAACTCTGCA CAAGCTTAAA ACATCATCAA ACTGACCCCA GTGCCAGCTA TTACATTGAC 
ACACAGAGCA ACTATGGGAC TTCTACCCCA TATTCCACTC CTCGGCTAAC ACTATCACAA 
ATGCCAGGGC AGCTACCTCA GATTCTGAGC CCACAGTCAA TACGGCTGTC GACTGAGCCA 
CAGCAGGTTA CCATCTGGAG TCCTTCTGCA GTTTGTGGTA TGACCACTCC ACCAACCTCC 
CCTGGAATCA TCCCATCGGA GTCATCCCAA TCTGCATCAC AACCTTACAA CAAAGCTTTT 
GGCCCATCTG GAGGGGGAAA AGGAACACCT TTGGGAACGC CTGCCACATC TCCTCCACCC 
TGCACTTCTG ATGACTTTGT GCATGTTTCG CTCCCTTCAG CTGCTGCCAC GCCTCCAAAA 
AAGGAGGACA AACCAGATCC TGGGAGGCCT TTACTGTACC GACAGCAAAA TGTCATAAAC 
AGCGATAAAT CATTGGACAC ACCTGGTAGT AAAAGTTCAG TAACCTTAAG TGATCTTCCA 
GAGTTTTTAG ATCGTTTGTC TTTTGAAGAT AGTGCTGAAA AGGACAGAGA GGAAGATGCA 
ATATCTAAAG AGATCTCCGA GATCACAACT GATGCTGAAC ACATGGTGCC TAGAGGAGGA 
TTTGACTCTC CGTTTTACCG CACAAATGAA AGTCTGTCAG GCTCTCAAAA GAAGACCCAT 
TCAGTAGTCT CTAGTGTTCA GGGACACAGT CAGACCTCTG AGCCTTTAAC ATCTTCTCTG 
GACAAGCCTG GGCCTGAGAG TGCGCAGGAA ACACCCAAAC AAACGTTTAC TCCCATAGAC 
AAGCCCTGCG GAGGCTCTGG TGAAAGCCCT GCTGGTAACA GGGAAGGAAC CTCTGGGGAG 
ATGAGTATTC TCACTCCCAG CCCTTGCAAA ATACCAGCAC AGAGAAGAGC AGTGTTTGGG 
AGCGGGCAGC CTCCACTGTA CGACCACCTT TTTGAGGTTG CGTTACCAAA GACTGCCTAC 
TTCTTTGTTG GCAAGAAGAC TGAGGAGCTG CTGAAGAAAG TCAAGGGAAA CCAGGACGAT 
GACTGCATGT GCTCTACTTC TCCCGTGGAA GTGCTGGACA GACTGATACA GCAAGGAGCT 
GATGCCCACA CTAAGGAGCT GAACAAATTG TCTCTGCCAA GCAAATCTGC TGACTGGACT 
CACTTCGGAG GTTCTCCCCC TTCAGATGAG ATTCACACCC TACGTAACCA ATTACTTTTG 
CTGCACAACC AGTTACTGTA TGAACGCTTC AAAAGACAGC AACATGCCCT ACGGAACCGG 
CGACTCTTGC GAAAAGTGAT TAAAGCAACA GCTCTGGAGG AACATAATGC TGCCATGAAA 
GATCAGCTGA AACTACAGGA AAAAGAAATC CAGGCCTTGA AACTGAGTCT GCAGAAAGAA 
CAGGCAAGAT ACCACCAGTT TCAGGAGGAG CATGAAAATA TAGTGGCTCA GCTTCACAGC 
CAGATCAGGC AGCTGCAACA TGACCGGGAG GAATTCTACA ACCAGAGCCA GGAACTGCAG 
ACCAAGCTGG AAGACTGCCG GAATATGATT GCAGATCTGA GGTTAGAGTT AAAGAAGGCT 
AACAATAAAG TGTGTCACAC TGAATTGCTT CTTAGCCAAG TTTCTCAAAA GCTTTCCAAC 
AGTGAATCGG TGCAACAGCA GATGGAGTTC TTGAACAGGC AGCTTCTGGT TCTTGGAGAG 
GTCAATGAGT TGTACCTGGA GCAGCTGCAG CACAAGCACA CAGACACTAC AAAGGAGGTT 
GAAATGTTGC ATGCTGCTTT TCGGAAAGAA CTGGAGAAAG CAAAATTGTG TGTTCAACAG 
CAAAGCCAAA AGCTTGATGC TTCTCAGAAA CGGATAGCTG AACTGGAATC TCAGCTTGCT 
AAAAAGGACC ACCTCTTTCT GGAGCAAAAG AAGTACCTGG AAGATGTCAA AATCCAAGCA 
AGAGGTCAGC TGCAAGCTGT AGAAAGTAGG TACAAGGCCC AGAAAAGAAT CACACAGGCA 
TTTGAGCTGG AGATCTTGGA TCTGTTTGGC AGGCTGGAGA AGAGCAGCCT ACTGAAAAAA 
CTTGAAGATG ATAAAACAGA AGCAGCTGAA GCAGCAGAAG AAAGGTTGGA TGGTGGTAAT 
GAAGATACTG TGGGAGGAAA CAGTGAAGAA ACAATTGGGA GAAATGGAGA GACCAAACCT 
CCCAGCACTC GAGGTAGCAG TAGCAGTAAG GGTGGCAGCA GCAGTGAGCT CTCCACCCCA 
GAAAAACCCC AGAACCAGAG ATTCAGCAGT CGCTGGGAGA CATCTGTGTT GCTGGAGCCC 
TCGACTACTG TGCCACTGAC TGTAGGTTCA CTTCCCAGCT CCAAGAGCTT CCTTGGAATG 
AAGGCACGAG AATTATTTCG CAACAAGAGT GAGAGCCAGT GTGATGAGGA GGGTGTAACC 
ATCAACAGGC TTTCTGATGC TCTAAAGACT GATCTATGTA AAGACCCAAG CCTGGAGATG 
AAGGCCCCTC CAAGCCCCGA TAACCTTAGC CTCTCGCCTA AGATCCAGGA AAGCAGTGTT 
GGACAGCTTC ATATCATGGA CTACAATGAA ACTCATCATG ACCACAGTTA A