IL10 cDNA ORF clone, Bubalus bubalis(water buffalo)

Gene
Clones
gRNAs

Summary

Gene Symbol	IL10
Entrez Gene ID	102400790
Full Name	interleukin 10
Synonyms	IL-10
Gene Type	protein-coding
Organism	Bubalus bubalis(water buffalo)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001290845.1	NP_001277774.1	interleukin-10 precursor

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Comparative assessment of Th1 and Th2 cytokines of swamp type buffalo and other bubaline breeds by molecular cloning, sequencing and phylogenetics.
Mingala CN, Odbileg R, Konnai S, Ohashi K, Onuma M
Veterinary immunology and immunopathology113(3-4)348-56(2006 Oct)

Molecular cloning and expression profile analysis of interleukin-10 and interleukin-18 cDNA of Indian water buffalo (Bubalus bubalis).
Premraj A, Sreekumar E, Nautiyal B, Rasool TJ
Veterinary immunology and immunopathology107(3-4)337-47(2005 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following IL10 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the IL10 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBu00142	NM_001290845.1 Latest version!	Bubalus bubalis interleukin 10 (IL10), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$104.30 $149.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

IL10 ( NM_001290845.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> NP_001277774.1 Bubalus bubalis interleukin 10 (IL10), mRNA.

Price & Availability↑

CloneID	OBu00142
Clone ID Related Accession (Same CDS sequence)	NM_001290845.1
Accession Version	NM_001290845.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 537bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-10-17
Organism	Bubalus bubalis(water buffalo)
Product	interleukin-10 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from EF362779.1. On Mar 20, 2014 this sequence version replaced XM_006043811.1. ##Evidence-Data-START## Transcript exon combination :: AY325267.1, EF362779.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA103886336, SAMEA103886344 [ECO:0000348] ##Evidence-Data-END##

ATGCATAGCT CAGCACTACT CTGTTGCCTG GTCTTCCTGG CTGGGGTGGC AGCCAGCCGA 
GATGCGAGCA CCCTGTCTGA CAGCAGCTGT ACCCAGTTCC CAACCAGCCT GCCCCACATG 
CTGCGGGAGC TCCGAGCTGC CTTCAGCAGG GTGAAGACTT TCTTTCAAAT GAAGGACCAA 
CTGGACAGCT TACTGTTGAC CCAGTCTCTG CTGGATGACT TTAAGGGTTA CCTGGGTTGC 
CAAGCCTTGT CGGAAATGAT CCAGTTTTAC CTGGAAGAGG TGATGCCACA GGCTGAGAAC 
CACGGGCCTG ACATCAAGGA GCACGTGAAC TCGCTGGGGG AGAAGCTGAA GACCCTCCGG 
CTGCGGCTGC GGCGCTGTCA TCGCTTTCTG CCCTGCGAAA ACAAGAGCAA GGCGGTGGAG 
CAGGTGAAGA AAGCCTTCAG TAAGCTCCAA GACAGGGGTG TCTACAAAGC CATGAGTGAG 
TTTGACATCT TCATCAACTA CATAGAAACC TACATGACAA CGAAGATGCA AAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001277774.1
CDS	1..537
Translation	MHSSALLCCLVFLAGVAASRDASTLSDSSCTQFPTSLPHMLRELRAAFSRVKTFFQMKDQLDSLLLTQSLLDDFKGYLGCQALSEMIQFYLEEVMPQAENHGPDIKEHVNSLGEKLKTLRLRLRRCHRFLPCENKSKAVEQVKKAFSKLQDRGVYKAMSEFDIFINYIETYMTTKMQK

Target ORF information:

RefSeq Version	NM_001290845.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis interleukin 10 (IL10), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001290845.1

ORF Insert Sequence:

ATGCATAGCT CAGCACTACT CTGTTGCCTG GTCTTCCTGG CTGGGGTGGC AGCCAGCCGA 
GATGCGAGCA CCCTGTCTGA CAGCAGCTGT ACCCAGTTCC CAACCAGCCT GCCCCACATG 
CTGCGGGAGC TCCGAGCTGC CTTCAGCAGG GTGAAGACTT TCTTTCAAAT GAAGGACCAA 
CTGGACAGCT TACTGTTGAC CCAGTCTCTG CTGGATGACT TTAAGGGTTA CCTGGGTTGC 
CAAGCCTTGT CGGAAATGAT CCAGTTTTAC CTGGAAGAGG TGATGCCACA GGCTGAGAAC 
CACGGGCCTG ACATCAAGGA GCACGTGAAC TCGCTGGGGG AGAAGCTGAA GACCCTCCGG 
CTGCGGCTGC GGCGCTGTCA TCGCTTTCTG CCCTGCGAAA ACAAGAGCAA GGCGGTGGAG 
CAGGTGAAGA AAGCCTTCAG TAAGCTCCAA GACAGGGGTG TCTACAAAGC CATGAGTGAG 
TTTGACATCT TCATCAACTA CATAGAAACC TACATGACAA CGAAGATGCA AAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Comparative assessment of Th1 and Th2 cytokines of swamp type buffalo and other bubaline breeds by molecular cloning, sequencing and phylogenetics.
Veterinary immunology and immunopathology113(3-4)348-56(2006 Oct)
Mingala CN,Odbileg R,Konnai S,Ohashi K,Onuma M

Molecular cloning and expression profile analysis of interleukin-10 and interleukin-18 cDNA of Indian water buffalo (Bubalus bubalis).
Veterinary immunology and immunopathology107(3-4)337-47(2005 Sep)
Premraj A,Sreekumar E,Nautiyal B,Rasool TJ