LOC100838079 cDNA ORF clone, Brachypodium distachyon(stiff brome)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC100838079
Entrez Gene ID	100838079
Full Name	interferon-induced, double-stranded RNA-activated protein kinase-like
Gene Type	protein-coding
Organism	Brachypodium distachyon(stiff brome)

mRNA and Protein(s)

mRNA	Protein	Name
XM_010232897.3	XP_010231199.3	cyclin-dependent kinase-like 4
XM_010232897.2	XP_010231199.2	interferon-induced, double-stranded RNA-activated protein kinase-like

Pathways from BioSystems
Gene Ontology

The following LOC100838079 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC100838079 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBr106014	XM_010232897.2 Latest version!	Brachypodium distachyon interferon-induced, double-stranded RNA-activated protein kinase-like (LOC100838079), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$174.30 $249.00
OBr268146	XM_010232897.3 Latest version!	Brachypodium distachyon cyclin-dependent kinase-like 4 (LOC100838079), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC100838079 ( XM_010232897.2 ) cDNA ORF clone, Brachypodium distachyon(stiff brome) -> XP_010231199.2 Brachypodium distachyon interferon-induced, double-stranded RNA-activated protein kinase-like (LOC100838079), mRNA.

Price & Availability↑

CloneID	OBr106014
Clone ID Related Accession (Same CDS sequence)	XM_010232897.2
Accession Version	XM_010232897.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 624bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-07
Organism	Brachypodium distachyon(stiff brome)
Product	interferon-induced, double-stranded RNA-activated protein kinase-like
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014576699.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 8, 2015 this sequence version replaced XM_010232897.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Brachypodium distachyon Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 7% of CDS bases ##RefSeq-Attributes-END##

ATGAAGTTGT GCAACAAGAC ACTAGAAAAC TATCTAAAGG AAACATCTCA AGTTGACCTG 
GAAACAAGAA AATCTATTTT TATGCAACTA ACAGCTGCTG TGGAATTTAT GCACCGAAAG 
GGAGTGGTGC ATAGGGATAT TAAACCTGGC AATGTGTTGT TAGATATCGA AAAAGATGGG 
CTGACTGTTA GGCTTGCAGA TTTTGGAATA TCTAAAAGAT TTGCACCACA TTCTGATTTT 
CCTGGTGATT GGTATGGGAG TCTACCTTAC CGTGCTCCTG AAATAGTCAA TAGCTCCCGA 
CGGCACAATG AAAAGGTCGA CATATTTTCA ATTGGAATGA TCTACTATGG ACTTTTTATT 
CCTGGAATGT ACAAGAGAAA AATGAAGTTG GATCAGCTCA GTGAAATTAT TCGTAAAGGC 
ATAGCAAAAT ATGATCAGAA AGGGCTCATA TTTGATCTCA AAGATCATCT AGATGGTACA 
GATATATTGG AAGACTGGGA AGGTGACTAC AGAATTCTGT ATCCAATGAT CAGATCAGAA 
CCGAGTGAAC GGCCATCGGC TACTCAGATA TTGGACCTGC TTGATCTAGA GAAAGGACTG 
CACTTCATGA AAGGAGTTGC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010231199.2
CDS	1..624
Translation	MKLCNKTLENYLKETSQVDLETRKSIFMQLTAAVEFMHRKGVVHRDIKPGNVLLDIEKDGLTVRLADFGISKRFAPHSDFPGDWYGSLPYRAPEIVNSSRRHNEKVDIFSIGMIYYGLFIPGMYKRKMKLDQLSEIIRKGIAKYDQKGLIFDLKDHLDGTDILEDWEGDYRILYPMIRSEPSERPSATQILDLLDLEKGLHFMKGVA

Target ORF information:

RefSeq Version	XM_010232897.2
Organism	Brachypodium distachyon(stiff brome)
Definition	Brachypodium distachyon interferon-induced, double-stranded RNA-activated protein kinase-like (LOC100838079), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010232897.2

ORF Insert Sequence:

ATGAAGTTGT GCAACAAGAC ACTAGAAAAC TATCTAAAGG AAACATCTCA AGTTGACCTG 
GAAACAAGAA AATCTATTTT TATGCAACTA ACAGCTGCTG TGGAATTTAT GCACCGAAAG 
GGAGTGGTGC ATAGGGATAT TAAACCTGGC AATGTGTTGT TAGATATCGA AAAAGATGGG 
CTGACTGTTA GGCTTGCAGA TTTTGGAATA TCTAAAAGAT TTGCACCACA TTCTGATTTT 
CCTGGTGATT GGTATGGGAG TCTACCTTAC CGTGCTCCTG AAATAGTCAA TAGCTCCCGA 
CGGCACAATG AAAAGGTCGA CATATTTTCA ATTGGAATGA TCTACTATGG ACTTTTTATT 
CCTGGAATGT ACAAGAGAAA AATGAAGTTG GATCAGCTCA GTGAAATTAT TCGTAAAGGC 
ATAGCAAAAT ATGATCAGAA AGGGCTCATA TTTGATCTCA AAGATCATCT AGATGGTACA 
GATATATTGG AAGACTGGGA AGGTGACTAC AGAATTCTGT ATCCAATGAT CAGATCAGAA 
CCGAGTGAAC GGCCATCGGC TACTCAGATA TTGGACCTGC TTGATCTAGA GAAAGGACTG 
CACTTCATGA AAGGAGTTGC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100838079 ( XM_010232897.3 ) cDNA ORF clone, Brachypodium distachyon(stiff brome) -> XP_010231199.3 Brachypodium distachyon cyclin-dependent kinase-like 4 (LOC100838079), mRNA.

Price & Availability↑

CloneID	OBr268146
Clone ID Related Accession (Same CDS sequence)	XM_010232897.3
Accession Version	XM_010232897.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1401bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-26
Organism	Brachypodium distachyon(stiff brome)
Product	cyclin-dependent kinase-like 4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_016132.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Mar 27, 2018 this sequence version replaced XM_010232897.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Brachypodium distachyon Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGGAG CCGGAGGGGA CAAGCGGGTG GAGCTCCTCC TACAGGGGTT TCGGAGCGAC 
GGCACCGCGA CTGGGGAGTA CCGCCTCGTC CCCCCATGGG AGCATCATCG TCTCCAACCG 
TGCGTCGCGG TGGCGTCCGT CTCCGAGGAC GCTACGGCCT CCCTGCCGAC GCCGCCAAAC 
CCAGGAGGCG CCCCTCCGGT GTTGGAGAAG CTGTCGCTCA GCATGGCGAG AGGAGGCGAT 
CGCGTGGCCT CGTTTGAGTT GGTGCCCGTC GCTTGTGCTG TGGATTTCCA TAAACTGGGT 
TGTGCTTCCC ACCTTACGCT GCGGATAAAT CCGATGGAGG GTCGGGCGAT TGTTCGCTTC 
TACAAAAACG AAGGATCATC ATCGCGATGG GCGTCTACTT GGGTTTACTC AATGAATGCA 
GTTTATCTTG AAGTCACTGA GATAACAAAA GATTTTGGAG AAGACCACCT CTATTTTCAT 
GGCCCAGCTT CGGAAGATAT ACCATGTCCC CCAATGACAA TCAGTTTCCA GAAAAACTTT 
TCCTGTGATG AAGAAATAGG AAGTGGATCA GAGGGCCAGG TCTATAAATG TCGCTCCGAT 
TTCATGGGGC CAAAAAAGTT TGCTGTAGTC AAGCAAGTTC CCGATACTTT TGAATATTTC 
AAGAATCCTG ATCTTAGCTG TGAAACTAGT GAGGTGAACA TATTGTCTAT GTTGAATCAT 
GATGGCATCG TGCAGTTCTA TCAAGCATGG ACAGAGAAAA GAACAATTTA CATTGGAATG 
AAGTTGTGCA ACAAGACACT AGAAAACTAT CTAAAGGAAA CATCTCAAGT TGACCTGGAA 
ACAAGAAAAT CTATTTTTAT GCAACTAACA GCTGCTGTGG AATTTATGCA CCGAAAGGGA 
GTGGTGCATA GGGATATTAA ACCTGGCAAT GTGTTGTTAG ATATCGAAAA AGATGGGCTG 
ACTGTTAGGC TTGCAGATTT TGGAATATCT AAAAGATTTG CACCACATTC TGATTTTCCT 
GGTGATTGGT ATGGGAGTCT ACCTTACCGT GCTCCTGAAA TAGTCAATAG CTCCCGACGG 
CACAATGAAA AGGTCGACAT ATTTTCAATT GGAATGATCT ACTATGGACT TTTTATTCCT 
GGAATGTACA AGAGAAAAAT GAAGTTGGAT CAGCTCAGTG AAATTATTCG TAAAGGCATA 
GCAAAATATG ATCAGAAAGG GCTCATATTT GATCTCAAAG ATCATCTAGA TGGTACAGAT 
ATATTGGAAG ACTGGGAAGG TGACTACAGA ATTCTGTATC CAATGATCAG ATCAGAACCG 
AGTGAACGGC CATCGGCTAC TCAGATATTG GACCTGCTTG ATCTAGAGAA AGGACTGCAC 
TTCATGAAAG GAGTTGCTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010231199.3
CDS	102..1502
Translation	MAGAGGDKRVELLLQGFRSDGTATGEYRLVPPWEHHRLQPCVAVASVSEDATASLPTPPNPGGAPPVLEKLSLSMARGGDRVASFELVPVACAVDFHKLGCASHLTLRINPMEGRAIVRFYKNEGSSSRWASTWVYSMNAVYLEVTEITKDFGEDHLYFHGPASEDIPCPPMTISFQKNFSCDEEIGSGSEGQVYKCRSDFMGPKKFAVVKQVPDTFEYFKNPDLSCETSEVNILSMLNHDGIVQFYQAWTEKRTIYIGMKLCNKTLENYLKETSQVDLETRKSIFMQLTAAVEFMHRKGVVHRDIKPGNVLLDIEKDGLTVRLADFGISKRFAPHSDFPGDWYGSLPYRAPEIVNSSRRHNEKVDIFSIGMIYYGLFIPGMYKRKMKLDQLSEIIRKGIAKYDQKGLIFDLKDHLDGTDILEDWEGDYRILYPMIRSEPSERPSATQILDLLDLEKGLHFMKGVA

Target ORF information:

RefSeq Version	XM_010232897.3
Organism	Brachypodium distachyon(stiff brome)
Definition	Brachypodium distachyon cyclin-dependent kinase-like 4 (LOC100838079), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010232897.3

ORF Insert Sequence:

ATGGCGGGAG CCGGAGGGGA CAAGCGGGTG GAGCTCCTCC TACAGGGGTT TCGGAGCGAC 
GGCACCGCGA CTGGGGAGTA CCGCCTCGTC CCCCCATGGG AGCATCATCG TCTCCAACCG 
TGCGTCGCGG TGGCGTCCGT CTCCGAGGAC GCTACGGCCT CCCTGCCGAC GCCGCCAAAC 
CCAGGAGGCG CCCCTCCGGT GTTGGAGAAG CTGTCGCTCA GCATGGCGAG AGGAGGCGAT 
CGCGTGGCCT CGTTTGAGTT GGTGCCCGTC GCTTGTGCTG TGGATTTCCA TAAACTGGGT 
TGTGCTTCCC ACCTTACGCT GCGGATAAAT CCGATGGAGG GTCGGGCGAT TGTTCGCTTC 
TACAAAAACG AAGGATCATC ATCGCGATGG GCGTCTACTT GGGTTTACTC AATGAATGCA 
GTTTATCTTG AAGTCACTGA GATAACAAAA GATTTTGGAG AAGACCACCT CTATTTTCAT 
GGCCCAGCTT CGGAAGATAT ACCATGTCCC CCAATGACAA TCAGTTTCCA GAAAAACTTT 
TCCTGTGATG AAGAAATAGG AAGTGGATCA GAGGGCCAGG TCTATAAATG TCGCTCCGAT 
TTCATGGGGC CAAAAAAGTT TGCTGTAGTC AAGCAAGTTC CCGATACTTT TGAATATTTC 
AAGAATCCTG ATCTTAGCTG TGAAACTAGT GAGGTGAACA TATTGTCTAT GTTGAATCAT 
GATGGCATCG TGCAGTTCTA TCAAGCATGG ACAGAGAAAA GAACAATTTA CATTGGAATG 
AAGTTGTGCA ACAAGACACT AGAAAACTAT CTAAAGGAAA CATCTCAAGT TGACCTGGAA 
ACAAGAAAAT CTATTTTTAT GCAACTAACA GCTGCTGTGG AATTTATGCA CCGAAAGGGA 
GTGGTGCATA GGGATATTAA ACCTGGCAAT GTGTTGTTAG ATATCGAAAA AGATGGGCTG 
ACTGTTAGGC TTGCAGATTT TGGAATATCT AAAAGATTTG CACCACATTC TGATTTTCCT 
GGTGATTGGT ATGGGAGTCT ACCTTACCGT GCTCCTGAAA TAGTCAATAG CTCCCGACGG 
CACAATGAAA AGGTCGACAT ATTTTCAATT GGAATGATCT ACTATGGACT TTTTATTCCT 
GGAATGTACA AGAGAAAAAT GAAGTTGGAT CAGCTCAGTG AAATTATTCG TAAAGGCATA 
GCAAAATATG ATCAGAAAGG GCTCATATTT GATCTCAAAG ATCATCTAGA TGGTACAGAT 
ATATTGGAAG ACTGGGAAGG TGACTACAGA ATTCTGTATC CAATGATCAG ATCAGAACCG 
AGTGAACGGC CATCGGCTAC TCAGATATTG GACCTGCTTG ATCTAGAGAA AGGACTGCAC 
TTCATGAAAG GAGTTGCTTG A