RSF1 gene cDNA ORF clone, Bos taurus(cattle)

mRNA	Protein	Name
XM_005197281.3	XP_005197338.1	remodeling and spacing factor 1 isoform X2
XM_010820856.3	XP_010819158.1	remodeling and spacing factor 1 isoform X1
XM_005226727.4	XP_005226784.1	remodeling and spacing factor 1 isoform X2
XM_002699063.5	XP_002699109.1	remodeling and spacing factor 1 isoform X3
XM_005226727.3	XP_005226784.1	remodeling and spacing factor 1 isoform X2
XM_010826221.2	XP_010824523.1	remodeling and spacing factor 1 isoform X1
XM_010820856.2	XP_010819158.1	remodeling and spacing factor 1 isoform X1
XM_002699063.4	XP_002699109.1	remodeling and spacing factor 1 isoform X3
XM_864539.7	XP_869632.1	remodeling and spacing factor 1 isoform X3

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa12597	XM_005197281.3 Latest version!	Bos taurus remodeling and spacing factor 1 (RSF1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OBa12597	XM_005226727.4 Latest version!	Bos taurus remodeling and spacing factor 1 (RSF1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OBa12598	XM_864539.7 Latest version!	Bos taurus remodeling and spacing factor 1 (RSF1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OBa12598	XM_002699063.5 Latest version!	Bos taurus remodeling and spacing factor 1 (RSF1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OBa55069	XM_010826221.2 Latest version!	Bos taurus remodeling and spacing factor 1 (RSF1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OBa55069	XM_010820856.3 Latest version!	Bos taurus remodeling and spacing factor 1 (RSF1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
View Old Accession Versions >>
OBa12597	XM_005226727.3	Bos taurus remodeling and spacing factor 1 (RSF1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OBa55069	XM_010820856.2	Bos taurus remodeling and spacing factor 1 (RSF1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OBa12598	XM_002699063.4	Bos taurus remodeling and spacing factor 1 (RSF1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
Hide Old Accession Versions >>

RSF1 ( XM_005197281.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005197338.1 Bos taurus remodeling and spacing factor 1 (RSF1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa12597
Clone ID Related Accession (Same CDS sequence)	XM_005197281.3 , XM_005226727.4 , XM_005226727.3
Accession Version	XM_005197281.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4356bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	remodeling and spacing factor 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646776.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005197281.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG CGGCGGCAGC GGCGGCGGCG ATGGCTCCTC CGGGCTGCCC GGGTTCGTGT 
CCCAACTTCG CCGTAGTCTG CTCTTTCTTG GAGCGCTACG GGCCGCTGCT CGACCTGCCT 
GAGTTGCCGT TCCCGGAGCT CGAGCGGGTG CTGCAAGCTC CGCCGCCGGA CGTCGGCAAC 
GGAGAAGTAC CTAAAGAATT GGTGGAGCTC CATTTGAAGT TGATGAGGAA GATTGGCAAA 
TCTGTTACAG CAGACAGATG GGAAAAATAC TTGATCAAGA TATGCCAAGA GTTTAATAGC 
ACCTGGGCGT GGGAGATGGA GAAGAAAGGC TATCTTGAAA TGAGTGTTGA ATGCAAGTTA 
GCACTCTTAA AGTACCTCTG TGAGTGTCAA TTTGATGACA ATCTCAAATT CAAGAATATT 
ATCAATGAGG AGGATGCCGA TACCATGCGT CTCCAGCCAA TTGGCCGTGA CAAAGATGGC 
CTCATGTACT GGTACCAGTT GGATCAAGAT CACAATGTCA GAATGTACAT AGAAGAACAA 
GATGATCAAG ATGGCTCTTC ATGGAAGTGC ATTGTGAGAA ATCGAAACGA GTTGGCTGAG 
ACACTTGCAC TCCTGAAAGC ACAAATTGAT CCTGTACTAT TGAAGAACTC TAGCCAACAG 
GACAGCTCTT CCCGGGAAAG TCCCAGCTTA GAAGAAGAGG AGACTAAAAA GGAGGAAGAA 
ACAACTAAAC AAGGTAACAG CTGTCTTGAA TCCCTTCAAG TCTGTCAAGG AACATTGCTT 
CTGCATTGTG ATTTTGAAGA GGAACAGAAA GGAAGTGCAA AGATGAAAAG TGAAGAGCGG 
CCAATAGATT CAGAAAAATG TTCTACACCC AGTGCTCTAG AAGAGACTAC TGTGAAAATA 
GAAAAAGAAG ATGAAAAGGA ACTTGTAAAA CTGCCAGTCA TAGTGAAGCT AGAAAAACCT 
TTTCCAGAAA GTGAAGAAAA AAAGATTATC AAAGAAGAAA GTGATTCCTT CAAGGAAAAT 
GTCAAACCTG TAAAAGTGGA AATGAAAGAA TGTAAAGCAG ATCCTAGAGA TATCAAAGGT 
AGTATGGAGA AGTTAGAGCC TGAGAGGCTA GAGTTTGTTG GCAATGTTAA ATCTTCTCAA 
GAAATTACTG AAAAGTCTAC TGAAGAAACC GAGAAACTTA AAAATGACCA GCAGGCCAAG 
ATTCCACTAA AAAAACGAGA AATTAAACTG AGTGATGATT TTGATAGTCC AATCAAAGGA 
CCTTTGTGTA AATCAGTTAC TCCAACAAAA GAGTTTTTGA AAGATGAAAT AAAACAAGAG 
GAAGAGACTT GTAAAAGGAT CTCTACAATC ACTGCCTTGG GTCATGAAGG GAAACAGCTG 
GTAAATGGAG AAGTTAGTGA TGAAAAGGTA ACTCCACATT TTAAGACAGA ACAGATGGAG 
ACAAAGTTTT ATGATTCAAA GGAAGATAGT TGCAGCCCCT CTAAGGACAG AAGTGTCATC 
ATGGAGGGAA ATGGAGCAGA GTCTGTAAAT TCTGTCATTC CAAGTGTAAA AATAGGTGAT 
CTTGAGAAAG AAGTGGTCCC TTTAGGGAAA GATACAGATA ATTCAATATC AGTCTTAGAG 
ACCCCGAGTC AGAAAGAGTA CATAGACGAA ACTGGTCCTT CAGAAATGGA AACCTCTCTT 
GAGACTGCTG AAGTAGCAAA GGATCTCTGT TTAAAAACTG CTTTATCTGC CACTGAATCC 
TATAGTATGA GAGTTGAAGA GAAGTCTCCC AAAAGTAAGA AGGATAAGCG CCCACCAGTC 
CTAGAGTGTC TTGAAAAGTC CAAAAAGACT TTTCTTGATA AGGACATACA AAGATTGAGT 
CCAATACCGG AGGAGGTTCC AAAGACAACT GTAGAATCAG AGAAGGCTGG GTCTCCTGAG 
GCAGCTGAAA CTTATCCGTC GTCTAACGTG ACTGTCCACT GTGAGAAACT AGCCTCAGAA 
GTTGAGTGTC AGAATACAAG TTCACTAGAA GGTCATTCCC TGGAGAAAGT AGACCCAGAA 
ATTTTAAAAG TGGATTCTGA ATCAACAAAG GTAGAAGTGG ATAATCTGGA CAATGCCCAG 
ACCTCTGGCA CAGGTGATCC TTCTGAGACA AAGGGTTCTA TGCAAAAAAG CAAATTGAAA 
TATAAGTTGA TTCCTGAAGA AGAAAACACT GCCTCAGAAA ACACAGAGAT AACCTCCGAA 
AGGCAGAAAG AAGGCATCAA ATTAACAATT AGGATATCTA GTCGGAAAAA GAAGTCAGAT 
TCTCCTCCCA AAATTTTAGA ACCAGAAACC AAGCAAGAGA AGACAGAAAA GGAAGAAGAG 
AAAACAAACG TTGGTCGTAC TTTGAGGAGG TCTCCAAGAA TATCTAGACC CACAGCAAAA 
GTGGCTGAAA TCAGAGATCA GAAAGCTGAT AAAAAAAGAG GGGAAGAAGA TGAAGTAGAA 
GAAGAGTCAG CAGCTTTGCA AAAGACAGAC AAAAAAGAAA ATTTGAAAAA AGCAGAGAAG 
GATACAAATT CTAAAGTAGC CAAGGTAAAG CCCAAAGGCA AAGTTCGATG GACTGGCTCT 
CGAACACGTG GCAGGTGGAA ATATTCCAGC AATGATGAAA GTGAAGGGTC TGACAGTGAA 
AAATCATCTG CAGCTTCAGA AGAGGAGGAA GAAAAGGAAA GTGAAGAAGC CATCCTAGCA 
GATGATGATG AACCATGCAA AAAATGTGGC CTTCCAAACC ATCCTGAATT AATCCTTCTG 
TGTGACTCTT GTGACAGTGG ATACCACACT GCCTGCCTTC GCCCTCCTCT AATGATTATT 
CCAGATGGAG AATGGTTCTG CCCCCCTTGC CAGCATAAAC TACTGTGTGA AAAATTAGAA 
GAACAATTGC AAGATTTGGA TGTTGCCTTA AAGAAGAAAG AGCGTGCTGA ACGCAGGAAA 
GAACGCTTGG TGTATGTGGG TATTAGTATT GAAAACATCA TTCCTCCACA AGAGCCAGAT 
TTTTCGGAAG ATCATGAAGA AAAGAAAAAA GATTCAAAAA AATCCAAAGC AAACTTGCTT 
GAAAGGAGGT CGACAAGAAC ACGGAAATGT ATAAGTTACA GATTTGATGA GTTTGACGAA 
GCAATTGATG AAGCTATAGA AGATGATATC AAAGAGGCTG ATGGAGGAGG AGTTGGCCGA 
GGAAAAGATA TCTCCACCAT CACAGGCCAC CGTGGAAAAG ACATCTCTAC TATTTTGGAT 
GAAGAAAGAA AAGAAAATAA ACGACCCCAG AGGGCAGCTG CTGCTCGCCG GAAGAAACGC 
CGGCGACTGA ATGATCTGGA CAGTGACAGC AACCTGGATG AAGAGGAGAG CGAGGATGAA 
TTCAAGATCA GTGATGGATC TCAGGATGAA TTTGTTGTAT CTGATGAGAA CCCAGATGAG 
AGTGAAGAAG ACCCACCATC TAATGATGAC AGCGACACTG ATTTCTGTAG CCGAAGACTA 
AGGCGACACC CCTCCCGGCC TATGAGGCAA AGCAGGCGTT TGCGGAGAAA GACCCCAAAG 
AGAAAGTGCT CTGACGATGA TGAGGAGGAA GAGTCTGAGG AGAACAGCAG AGACTCTGAA 
AGTGACTTTA GTGATGACTT TAGCGATGAT TTTGTAGAAA CTCGGCGAAG AAGGTCTAGA 
AGGAACCAGA AGAGACAAAT TAACTACAAG GAAGATTCAG AAAGTGATGG CTCCCAGAAG 
AGTTTACGAC GTGGTAAAGA AATCAGACGA GTTCACAAGC GTAGGCTCTC TAGCTCAGAT 
AGTGAAGAGA GCTATATGTC CAAGAACTCT GAAGATGATG AGCTAGCTAA AGAATCAAAG 
CGGTCAGTTC GAAAACGGGG TCGAAGCACA GATGAGTATT CCGAAGCAGA TGAGGAGGAA 
GAAGAAGGTA AACCATCCCG AAAACGGCTA CACCGGATCG AGACAGATGA AGAGGAGAGT 
TGTGACAATG CTCATGGAGA TGCAGATCAA CCTGCCCATG ACCGCCAGCC CAGGGTCCTG 
CCCTCAGAAC AAGACAGCAC CAAAAAGCCC TATCGGATAG ACAGTGACGA GGAAGAGGAC 
TTTGAAAATG TAGGCAAGGT GGGGAGCCCA TTGGACTATA GCTTAGTGGA CCTACCTTCC 
ACCAATGGAC AGAGTCCTGG CAAAGCCATT GAAAACTTGA TTGGCAAGCC AGCTGAGAAG 
CCTCAGACCC CCAAGGACAG CAGCACAGCC AGTGCAAGCC TGGCCCCCAA TGGGACAAGT 
GGTGGGCAGG AGGCAGGGGC CCCAGAAGAG GACGAAGATG AGCTTTTAAG AGTGACTGAC 
CTTGTTGATT ATGTCTGTAA CAGTGAACAG TTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005197338.1
CDS	284..4639
Translation	MAAAAAAAAAMAPPGCPGSCPNFAVVCSFLERYGPLLDLPELPFPELERVLQAPPPDVGNGEVPKELVELHLKLMRKIGKSVTADRWEKYLIKICQEFNSTWAWEMEKKGYLEMSVECKLALLKYLCECQFDDNLKFKNIINEEDADTMRLQPIGRDKDGLMYWYQLDQDHNVRMYIEEQDDQDGSSWKCIVRNRNELAETLALLKAQIDPVLLKNSSQQDSSSRESPSLEEEETKKEEETTKQGNSCLESLQVCQGTLLLHCDFEEEQKGSAKMKSEERPIDSEKCSTPSALEETTVKIEKEDEKELVKLPVIVKLEKPFPESEEKKIIKEESDSFKENVKPVKVEMKECKADPRDIKGSMEKLEPERLEFVGNVKSSQEITEKSTEETEKLKNDQQAKIPLKKREIKLSDDFDSPIKGPLCKSVTPTKEFLKDEIKQEEETCKRISTITALGHEGKQLVNGEVSDEKVTPHFKTEQMETKFYDSKEDSCSPSKDRSVIMEGNGAESVNSVIPSVKIGDLEKEVVPLGKDTDNSISVLETPSQKEYIDETGPSEMETSLETAEVAKDLCLKTALSATESYSMRVEEKSPKSKKDKRPPVLECLEKSKKTFLDKDIQRLSPIPEEVPKTTVESEKAGSPEAAETYPSSNVTVHCEKLASEVECQNTSSLEGHSLEKVDPEILKVDSESTKVEVDNLDNAQTSGTGDPSETKGSMQKSKLKYKLIPEEENTASENTEITSERQKEGIKLTIRISSRKKKSDSPPKILEPETKQEKTEKEEEKTNVGRTLRRSPRISRPTAKVAEIRDQKADKKRGEEDEVEEESAALQKTDKKENLKKAEKDTNSKVAKVKPKGKVRWTGSRTRGRWKYSSNDESEGSDSEKSSAASEEEEEKESEEAILADDDEPCKKCGLPNHPELILLCDSCDSGYHTACLRPPLMIIPDGEWFCPPCQHKLLCEKLEEQLQDLDVALKKKERAERRKERLVYVGISIENIIPPQEPDFSEDHEEKKKDSKKSKANLLERRSTRTRKCISYRFDEFDEAIDEAIEDDIKEADGGGVGRGKDISTITGHRGKDISTILDEERKENKRPQRAAAARRKKRRRLNDLDSDSNLDEEESEDEFKISDGSQDEFVVSDENPDESEEDPPSNDDSDTDFCSRRLRRHPSRPMRQSRRLRRKTPKRKCSDDDEEEESEENSRDSESDFSDDFSDDFVETRRRRSRRNQKRQINYKEDSESDGSQKSLRRGKEIRRVHKRRLSSSDSEESYMSKNSEDDELAKESKRSVRKRGRSTDEYSEADEEEEEGKPSRKRLHRIETDEEESCDNAHGDADQPAHDRQPRVLPSEQDSTKKPYRIDSDEEEDFENVGKVGSPLDYSLVDLPSTNGQSPGKAIENLIGKPAEKPQTPKDSSTASASLAPNGTSGGQEAGAPEEDEDELLRVTDLVDYVCNSEQL

Target ORF information:

RefSeq Version	XM_005197281.3
Organism	Bos taurus(cattle)
Definition	Bos taurus remodeling and spacing factor 1 (RSF1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005197281.3

ORF Insert Sequence:

ATGGCGGCGG CGGCGGCAGC GGCGGCGGCG ATGGCTCCTC CGGGCTGCCC GGGTTCGTGT 
CCCAACTTCG CCGTAGTCTG CTCTTTCTTG GAGCGCTACG GGCCGCTGCT CGACCTGCCT 
GAGTTGCCGT TCCCGGAGCT CGAGCGGGTG CTGCAAGCTC CGCCGCCGGA CGTCGGCAAC 
GGAGAAGTAC CTAAAGAATT GGTGGAGCTC CATTTGAAGT TGATGAGGAA GATTGGCAAA 
TCTGTTACAG CAGACAGATG GGAAAAATAC TTGATCAAGA TATGCCAAGA GTTTAATAGC 
ACCTGGGCGT GGGAGATGGA GAAGAAAGGC TATCTTGAAA TGAGTGTTGA ATGCAAGTTA 
GCACTCTTAA AGTACCTCTG TGAGTGTCAA TTTGATGACA ATCTCAAATT CAAGAATATT 
ATCAATGAGG AGGATGCCGA TACCATGCGT CTCCAGCCAA TTGGCCGTGA CAAAGATGGC 
CTCATGTACT GGTACCAGTT GGATCAAGAT CACAATGTCA GAATGTACAT AGAAGAACAA 
GATGATCAAG ATGGCTCTTC ATGGAAGTGC ATTGTGAGAA ATCGAAACGA GTTGGCTGAG 
ACACTTGCAC TCCTGAAAGC ACAAATTGAT CCTGTACTAT TGAAGAACTC TAGCCAACAG 
GACAGCTCTT CCCGGGAAAG TCCCAGCTTA GAAGAAGAGG AGACTAAAAA GGAGGAAGAA 
ACAACTAAAC AAGGTAACAG CTGTCTTGAA TCCCTTCAAG TCTGTCAAGG AACATTGCTT 
CTGCATTGTG ATTTTGAAGA GGAACAGAAA GGAAGTGCAA AGATGAAAAG TGAAGAGCGG 
CCAATAGATT CAGAAAAATG TTCTACACCC AGTGCTCTAG AAGAGACTAC TGTGAAAATA 
GAAAAAGAAG ATGAAAAGGA ACTTGTAAAA CTGCCAGTCA TAGTGAAGCT AGAAAAACCT 
TTTCCAGAAA GTGAAGAAAA AAAGATTATC AAAGAAGAAA GTGATTCCTT CAAGGAAAAT 
GTCAAACCTG TAAAAGTGGA AATGAAAGAA TGTAAAGCAG ATCCTAGAGA TATCAAAGGT 
AGTATGGAGA AGTTAGAGCC TGAGAGGCTA GAGTTTGTTG GCAATGTTAA ATCTTCTCAA 
GAAATTACTG AAAAGTCTAC TGAAGAAACC GAGAAACTTA AAAATGACCA GCAGGCCAAG 
ATTCCACTAA AAAAACGAGA AATTAAACTG AGTGATGATT TTGATAGTCC AATCAAAGGA 
CCTTTGTGTA AATCAGTTAC TCCAACAAAA GAGTTTTTGA AAGATGAAAT AAAACAAGAG 
GAAGAGACTT GTAAAAGGAT CTCTACAATC ACTGCCTTGG GTCATGAAGG GAAACAGCTG 
GTAAATGGAG AAGTTAGTGA TGAAAAGGTA ACTCCACATT TTAAGACAGA ACAGATGGAG 
ACAAAGTTTT ATGATTCAAA GGAAGATAGT TGCAGCCCCT CTAAGGACAG AAGTGTCATC 
ATGGAGGGAA ATGGAGCAGA GTCTGTAAAT TCTGTCATTC CAAGTGTAAA AATAGGTGAT 
CTTGAGAAAG AAGTGGTCCC TTTAGGGAAA GATACAGATA ATTCAATATC AGTCTTAGAG 
ACCCCGAGTC AGAAAGAGTA CATAGACGAA ACTGGTCCTT CAGAAATGGA AACCTCTCTT 
GAGACTGCTG AAGTAGCAAA GGATCTCTGT TTAAAAACTG CTTTATCTGC CACTGAATCC 
TATAGTATGA GAGTTGAAGA GAAGTCTCCC AAAAGTAAGA AGGATAAGCG CCCACCAGTC 
CTAGAGTGTC TTGAAAAGTC CAAAAAGACT TTTCTTGATA AGGACATACA AAGATTGAGT 
CCAATACCGG AGGAGGTTCC AAAGACAACT GTAGAATCAG AGAAGGCTGG GTCTCCTGAG 
GCAGCTGAAA CTTATCCGTC GTCTAACGTG ACTGTCCACT GTGAGAAACT AGCCTCAGAA 
GTTGAGTGTC AGAATACAAG TTCACTAGAA GGTCATTCCC TGGAGAAAGT AGACCCAGAA 
ATTTTAAAAG TGGATTCTGA ATCAACAAAG GTAGAAGTGG ATAATCTGGA CAATGCCCAG 
ACCTCTGGCA CAGGTGATCC TTCTGAGACA AAGGGTTCTA TGCAAAAAAG CAAATTGAAA 
TATAAGTTGA TTCCTGAAGA AGAAAACACT GCCTCAGAAA ACACAGAGAT AACCTCCGAA 
AGGCAGAAAG AAGGCATCAA ATTAACAATT AGGATATCTA GTCGGAAAAA GAAGTCAGAT 
TCTCCTCCCA AAATTTTAGA ACCAGAAACC AAGCAAGAGA AGACAGAAAA GGAAGAAGAG 
AAAACAAACG TTGGTCGTAC TTTGAGGAGG TCTCCAAGAA TATCTAGACC CACAGCAAAA 
GTGGCTGAAA TCAGAGATCA GAAAGCTGAT AAAAAAAGAG GGGAAGAAGA TGAAGTAGAA 
GAAGAGTCAG CAGCTTTGCA AAAGACAGAC AAAAAAGAAA ATTTGAAAAA AGCAGAGAAG 
GATACAAATT CTAAAGTAGC CAAGGTAAAG CCCAAAGGCA AAGTTCGATG GACTGGCTCT 
CGAACACGTG GCAGGTGGAA ATATTCCAGC AATGATGAAA GTGAAGGGTC TGACAGTGAA 
AAATCATCTG CAGCTTCAGA AGAGGAGGAA GAAAAGGAAA GTGAAGAAGC CATCCTAGCA 
GATGATGATG AACCATGCAA AAAATGTGGC CTTCCAAACC ATCCTGAATT AATCCTTCTG 
TGTGACTCTT GTGACAGTGG ATACCACACT GCCTGCCTTC GCCCTCCTCT AATGATTATT 
CCAGATGGAG AATGGTTCTG CCCCCCTTGC CAGCATAAAC TACTGTGTGA AAAATTAGAA 
GAACAATTGC AAGATTTGGA TGTTGCCTTA AAGAAGAAAG AGCGTGCTGA ACGCAGGAAA 
GAACGCTTGG TGTATGTGGG TATTAGTATT GAAAACATCA TTCCTCCACA AGAGCCAGAT 
TTTTCGGAAG ATCATGAAGA AAAGAAAAAA GATTCAAAAA AATCCAAAGC AAACTTGCTT 
GAAAGGAGGT CGACAAGAAC ACGGAAATGT ATAAGTTACA GATTTGATGA GTTTGACGAA 
GCAATTGATG AAGCTATAGA AGATGATATC AAAGAGGCTG ATGGAGGAGG AGTTGGCCGA 
GGAAAAGATA TCTCCACCAT CACAGGCCAC CGTGGAAAAG ACATCTCTAC TATTTTGGAT 
GAAGAAAGAA AAGAAAATAA ACGACCCCAG AGGGCAGCTG CTGCTCGCCG GAAGAAACGC 
CGGCGACTGA ATGATCTGGA CAGTGACAGC AACCTGGATG AAGAGGAGAG CGAGGATGAA 
TTCAAGATCA GTGATGGATC TCAGGATGAA TTTGTTGTAT CTGATGAGAA CCCAGATGAG 
AGTGAAGAAG ACCCACCATC TAATGATGAC AGCGACACTG ATTTCTGTAG CCGAAGACTA 
AGGCGACACC CCTCCCGGCC TATGAGGCAA AGCAGGCGTT TGCGGAGAAA GACCCCAAAG 
AGAAAGTGCT CTGACGATGA TGAGGAGGAA GAGTCTGAGG AGAACAGCAG AGACTCTGAA 
AGTGACTTTA GTGATGACTT TAGCGATGAT TTTGTAGAAA CTCGGCGAAG AAGGTCTAGA 
AGGAACCAGA AGAGACAAAT TAACTACAAG GAAGATTCAG AAAGTGATGG CTCCCAGAAG 
AGTTTACGAC GTGGTAAAGA AATCAGACGA GTTCACAAGC GTAGGCTCTC TAGCTCAGAT 
AGTGAAGAGA GCTATATGTC CAAGAACTCT GAAGATGATG AGCTAGCTAA AGAATCAAAG 
CGGTCAGTTC GAAAACGGGG TCGAAGCACA GATGAGTATT CCGAAGCAGA TGAGGAGGAA 
GAAGAAGGTA AACCATCCCG AAAACGGCTA CACCGGATCG AGACAGATGA AGAGGAGAGT 
TGTGACAATG CTCATGGAGA TGCAGATCAA CCTGCCCATG ACCGCCAGCC CAGGGTCCTG 
CCCTCAGAAC AAGACAGCAC CAAAAAGCCC TATCGGATAG ACAGTGACGA GGAAGAGGAC 
TTTGAAAATG TAGGCAAGGT GGGGAGCCCA TTGGACTATA GCTTAGTGGA CCTACCTTCC 
ACCAATGGAC AGAGTCCTGG CAAAGCCATT GAAAACTTGA TTGGCAAGCC AGCTGAGAAG 
CCTCAGACCC CCAAGGACAG CAGCACAGCC AGTGCAAGCC TGGCCCCCAA TGGGACAAGT 
GGTGGGCAGG AGGCAGGGGC CCCAGAAGAG GACGAAGATG AGCTTTTAAG AGTGACTGAC 
CTTGTTGATT ATGTCTGTAA CAGTGAACAG TTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RSF1 ( XM_005226727.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005226784.1 Bos taurus remodeling and spacing factor 1 (RSF1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa12597
Clone ID Related Accession (Same CDS sequence)	XM_005197281.3 , XM_005226727.4 , XM_005226727.3
Accession Version	XM_005226727.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4356bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	remodeling and spacing factor 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037356.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005226727.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG CGGCGGCAGC GGCGGCGGCG ATGGCTCCTC CGGGCTGCCC GGGTTCGTGT 
CCCAACTTCG CCGTAGTCTG CTCTTTCTTG GAGCGCTACG GGCCGCTGCT CGACCTGCCT 
GAGTTGCCGT TCCCGGAGCT CGAGCGGGTG CTGCAAGCTC CGCCGCCGGA CGTCGGCAAC 
GGAGAAGTAC CTAAAGAATT GGTGGAGCTC CATTTGAAGT TGATGAGGAA GATTGGCAAA 
TCTGTTACAG CAGACAGATG GGAAAAATAC TTGATCAAGA TATGCCAAGA GTTTAATAGC 
ACCTGGGCGT GGGAGATGGA GAAGAAAGGC TATCTTGAAA TGAGTGTTGA ATGCAAGTTA 
GCACTCTTAA AGTACCTCTG TGAGTGTCAA TTTGATGACA ATCTCAAATT CAAGAATATT 
ATCAATGAGG AGGATGCCGA TACCATGCGT CTCCAGCCAA TTGGCCGTGA CAAAGATGGC 
CTCATGTACT GGTACCAGTT GGATCAAGAT CACAATGTCA GAATGTACAT AGAAGAACAA 
GATGATCAAG ATGGCTCTTC ATGGAAGTGC ATTGTGAGAA ATCGAAACGA GTTGGCTGAG 
ACACTTGCAC TCCTGAAAGC ACAAATTGAT CCTGTACTAT TGAAGAACTC TAGCCAACAG 
GACAGCTCTT CCCGGGAAAG TCCCAGCTTA GAAGAAGAGG AGACTAAAAA GGAGGAAGAA 
ACAACTAAAC AAGGTAACAG CTGTCTTGAA TCCCTTCAAG TCTGTCAAGG AACATTGCTT 
CTGCATTGTG ATTTTGAAGA GGAACAGAAA GGAAGTGCAA AGATGAAAAG TGAAGAGCGG 
CCAATAGATT CAGAAAAATG TTCTACACCC AGTGCTCTAG AAGAGACTAC TGTGAAAATA 
GAAAAAGAAG ATGAAAAGGA ACTTGTAAAA CTGCCAGTCA TAGTGAAGCT AGAAAAACCT 
TTTCCAGAAA GTGAAGAAAA AAAGATTATC AAAGAAGAAA GTGATTCCTT CAAGGAAAAT 
GTCAAACCTG TAAAAGTGGA AATGAAAGAA TGTAAAGCAG ATCCTAGAGA TATCAAAGGT 
AGTATGGAGA AGTTAGAGCC TGAGAGGCTA GAGTTTGTTG GCAATGTTAA ATCTTCTCAA 
GAAATTACTG AAAAGTCTAC TGAAGAAACC GAGAAACTTA AAAATGACCA GCAGGCCAAG 
ATTCCACTAA AAAAACGAGA AATTAAACTG AGTGATGATT TTGATAGTCC AATCAAAGGA 
CCTTTGTGTA AATCAGTTAC TCCAACAAAA GAGTTTTTGA AAGATGAAAT AAAACAAGAG 
GAAGAGACTT GTAAAAGGAT CTCTACAATC ACTGCCTTGG GTCATGAAGG GAAACAGCTG 
GTAAATGGAG AAGTTAGTGA TGAAAAGGTA ACTCCACATT TTAAGACAGA ACAGATGGAG 
ACAAAGTTTT ATGATTCAAA GGAAGATAGT TGCAGCCCCT CTAAGGACAG AAGTGTCATC 
ATGGAGGGAA ATGGAGCAGA GTCTGTAAAT TCTGTCATTC CAAGTGTAAA AATAGGTGAT 
CTTGAGAAAG AAGTGGTCCC TTTAGGGAAA GATACAGATA ATTCAATATC AGTCTTAGAG 
ACCCCGAGTC AGAAAGAGTA CATAGACGAA ACTGGTCCTT CAGAAATGGA AACCTCTCTT 
GAGACTGCTG AAGTAGCAAA GGATCTCTGT TTAAAAACTG CTTTATCTGC CACTGAATCC 
TATAGTATGA GAGTTGAAGA GAAGTCTCCC AAAAGTAAGA AGGATAAGCG CCCACCAGTC 
CTAGAGTGTC TTGAAAAGTC CAAAAAGACT TTTCTTGATA AGGACATACA AAGATTGAGT 
CCAATACCGG AGGAGGTTCC AAAGACAACT GTAGAATCAG AGAAGGCTGG GTCTCCTGAG 
GCAGCTGAAA CTTATCCGTC GTCTAACGTG ACTGTCCACT GTGAGAAACT AGCCTCAGAA 
GTTGAGTGTC AGAATACAAG TTCACTAGAA GGTCATTCCC TGGAGAAAGT AGACCCAGAA 
ATTTTAAAAG TGGATTCTGA ATCAACAAAG GTAGAAGTGG ATAATCTGGA CAATGCCCAG 
ACCTCTGGCA CAGGTGATCC TTCTGAGACA AAGGGTTCTA TGCAAAAAAG CAAATTGAAA 
TATAAGTTGA TTCCTGAAGA AGAAAACACT GCCTCAGAAA ACACAGAGAT AACCTCCGAA 
AGGCAGAAAG AAGGCATCAA ATTAACAATT AGGATATCTA GTCGGAAAAA GAAGTCAGAT 
TCTCCTCCCA AAATTTTAGA ACCAGAAACC AAGCAAGAGA AGACAGAAAA GGAAGAAGAG 
AAAACAAACG TTGGTCGTAC TTTGAGGAGG TCTCCAAGAA TATCTAGACC CACAGCAAAA 
GTGGCTGAAA TCAGAGATCA GAAAGCTGAT AAAAAAAGAG GGGAAGAAGA TGAAGTAGAA 
GAAGAGTCAG CAGCTTTGCA AAAGACAGAC AAAAAAGAAA ATTTGAAAAA AGCAGAGAAG 
GATACAAATT CTAAAGTAGC CAAGGTAAAG CCCAAAGGCA AAGTTCGATG GACTGGCTCT 
CGAACACGTG GCAGGTGGAA ATATTCCAGC AATGATGAAA GTGAAGGGTC TGACAGTGAA 
AAATCATCTG CAGCTTCAGA AGAGGAGGAA GAAAAGGAAA GTGAAGAAGC CATCCTAGCA 
GATGATGATG AACCATGCAA AAAATGTGGC CTTCCAAACC ATCCTGAATT AATCCTTCTG 
TGTGACTCTT GTGACAGTGG ATACCACACT GCCTGCCTTC GCCCTCCTCT AATGATTATT 
CCAGATGGAG AATGGTTCTG CCCCCCTTGC CAGCATAAAC TACTGTGTGA AAAATTAGAA 
GAACAATTGC AAGATTTGGA TGTTGCCTTA AAGAAGAAAG AGCGTGCTGA ACGCAGGAAA 
GAACGCTTGG TGTATGTGGG TATTAGTATT GAAAACATCA TTCCTCCACA AGAGCCAGAT 
TTTTCGGAAG ATCATGAAGA AAAGAAAAAA GATTCAAAAA AATCCAAAGC AAACTTGCTT 
GAAAGGAGGT CGACAAGAAC ACGGAAATGT ATAAGTTACA GATTTGATGA GTTTGACGAA 
GCAATTGATG AAGCTATAGA AGATGATATC AAAGAGGCTG ATGGAGGAGG AGTTGGCCGA 
GGAAAAGATA TCTCCACCAT CACAGGCCAC CGTGGAAAAG ACATCTCTAC TATTTTGGAT 
GAAGAAAGAA AAGAAAATAA ACGACCCCAG AGGGCAGCTG CTGCTCGCCG GAAGAAACGC 
CGGCGACTGA ATGATCTGGA CAGTGACAGC AACCTGGATG AAGAGGAGAG CGAGGATGAA 
TTCAAGATCA GTGATGGATC TCAGGATGAA TTTGTTGTAT CTGATGAGAA CCCAGATGAG 
AGTGAAGAAG ACCCACCATC TAATGATGAC AGCGACACTG ATTTCTGTAG CCGAAGACTA 
AGGCGACACC CCTCCCGGCC TATGAGGCAA AGCAGGCGTT TGCGGAGAAA GACCCCAAAG 
AGAAAGTGCT CTGACGATGA TGAGGAGGAA GAGTCTGAGG AGAACAGCAG AGACTCTGAA 
AGTGACTTTA GTGATGACTT TAGCGATGAT TTTGTAGAAA CTCGGCGAAG AAGGTCTAGA 
AGGAACCAGA AGAGACAAAT TAACTACAAG GAAGATTCAG AAAGTGATGG CTCCCAGAAG 
AGTTTACGAC GTGGTAAAGA AATCAGACGA GTTCACAAGC GTAGGCTCTC TAGCTCAGAT 
AGTGAAGAGA GCTATATGTC CAAGAACTCT GAAGATGATG AGCTAGCTAA AGAATCAAAG 
CGGTCAGTTC GAAAACGGGG TCGAAGCACA GATGAGTATT CCGAAGCAGA TGAGGAGGAA 
GAAGAAGGTA AACCATCCCG AAAACGGCTA CACCGGATCG AGACAGATGA AGAGGAGAGT 
TGTGACAATG CTCATGGAGA TGCAGATCAA CCTGCCCATG ACCGCCAGCC CAGGGTCCTG 
CCCTCAGAAC AAGACAGCAC CAAAAAGCCC TATCGGATAG ACAGTGACGA GGAAGAGGAC 
TTTGAAAATG TAGGCAAGGT GGGGAGCCCA TTGGACTATA GCTTAGTGGA CCTACCTTCC 
ACCAATGGAC AGAGTCCTGG CAAAGCCATT GAAAACTTGA TTGGCAAGCC AGCTGAGAAG 
CCTCAGACCC CCAAGGACAG CAGCACAGCC AGTGCAAGCC TGGCCCCCAA TGGGACAAGT 
GGTGGGCAGG AGGCAGGGGC CCCAGAAGAG GACGAAGATG AGCTTTTAAG AGTGACTGAC 
CTTGTTGATT ATGTCTGTAA CAGTGAACAG TTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005226784.1
CDS	280..4635
Translation	MAAAAAAAAAMAPPGCPGSCPNFAVVCSFLERYGPLLDLPELPFPELERVLQAPPPDVGNGEVPKELVELHLKLMRKIGKSVTADRWEKYLIKICQEFNSTWAWEMEKKGYLEMSVECKLALLKYLCECQFDDNLKFKNIINEEDADTMRLQPIGRDKDGLMYWYQLDQDHNVRMYIEEQDDQDGSSWKCIVRNRNELAETLALLKAQIDPVLLKNSSQQDSSSRESPSLEEEETKKEEETTKQGNSCLESLQVCQGTLLLHCDFEEEQKGSAKMKSEERPIDSEKCSTPSALEETTVKIEKEDEKELVKLPVIVKLEKPFPESEEKKIIKEESDSFKENVKPVKVEMKECKADPRDIKGSMEKLEPERLEFVGNVKSSQEITEKSTEETEKLKNDQQAKIPLKKREIKLSDDFDSPIKGPLCKSVTPTKEFLKDEIKQEEETCKRISTITALGHEGKQLVNGEVSDEKVTPHFKTEQMETKFYDSKEDSCSPSKDRSVIMEGNGAESVNSVIPSVKIGDLEKEVVPLGKDTDNSISVLETPSQKEYIDETGPSEMETSLETAEVAKDLCLKTALSATESYSMRVEEKSPKSKKDKRPPVLECLEKSKKTFLDKDIQRLSPIPEEVPKTTVESEKAGSPEAAETYPSSNVTVHCEKLASEVECQNTSSLEGHSLEKVDPEILKVDSESTKVEVDNLDNAQTSGTGDPSETKGSMQKSKLKYKLIPEEENTASENTEITSERQKEGIKLTIRISSRKKKSDSPPKILEPETKQEKTEKEEEKTNVGRTLRRSPRISRPTAKVAEIRDQKADKKRGEEDEVEEESAALQKTDKKENLKKAEKDTNSKVAKVKPKGKVRWTGSRTRGRWKYSSNDESEGSDSEKSSAASEEEEEKESEEAILADDDEPCKKCGLPNHPELILLCDSCDSGYHTACLRPPLMIIPDGEWFCPPCQHKLLCEKLEEQLQDLDVALKKKERAERRKERLVYVGISIENIIPPQEPDFSEDHEEKKKDSKKSKANLLERRSTRTRKCISYRFDEFDEAIDEAIEDDIKEADGGGVGRGKDISTITGHRGKDISTILDEERKENKRPQRAAAARRKKRRRLNDLDSDSNLDEEESEDEFKISDGSQDEFVVSDENPDESEEDPPSNDDSDTDFCSRRLRRHPSRPMRQSRRLRRKTPKRKCSDDDEEEESEENSRDSESDFSDDFSDDFVETRRRRSRRNQKRQINYKEDSESDGSQKSLRRGKEIRRVHKRRLSSSDSEESYMSKNSEDDELAKESKRSVRKRGRSTDEYSEADEEEEEGKPSRKRLHRIETDEEESCDNAHGDADQPAHDRQPRVLPSEQDSTKKPYRIDSDEEEDFENVGKVGSPLDYSLVDLPSTNGQSPGKAIENLIGKPAEKPQTPKDSSTASASLAPNGTSGGQEAGAPEEDEDELLRVTDLVDYVCNSEQL

Target ORF information:

RefSeq Version	XM_005226727.4
Organism	Bos taurus(cattle)
Definition	Bos taurus remodeling and spacing factor 1 (RSF1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005226727.4

ORF Insert Sequence:

ATGGCGGCGG CGGCGGCAGC GGCGGCGGCG ATGGCTCCTC CGGGCTGCCC GGGTTCGTGT 
CCCAACTTCG CCGTAGTCTG CTCTTTCTTG GAGCGCTACG GGCCGCTGCT CGACCTGCCT 
GAGTTGCCGT TCCCGGAGCT CGAGCGGGTG CTGCAAGCTC CGCCGCCGGA CGTCGGCAAC 
GGAGAAGTAC CTAAAGAATT GGTGGAGCTC CATTTGAAGT TGATGAGGAA GATTGGCAAA 
TCTGTTACAG CAGACAGATG GGAAAAATAC TTGATCAAGA TATGCCAAGA GTTTAATAGC 
ACCTGGGCGT GGGAGATGGA GAAGAAAGGC TATCTTGAAA TGAGTGTTGA ATGCAAGTTA 
GCACTCTTAA AGTACCTCTG TGAGTGTCAA TTTGATGACA ATCTCAAATT CAAGAATATT 
ATCAATGAGG AGGATGCCGA TACCATGCGT CTCCAGCCAA TTGGCCGTGA CAAAGATGGC 
CTCATGTACT GGTACCAGTT GGATCAAGAT CACAATGTCA GAATGTACAT AGAAGAACAA 
GATGATCAAG ATGGCTCTTC ATGGAAGTGC ATTGTGAGAA ATCGAAACGA GTTGGCTGAG 
ACACTTGCAC TCCTGAAAGC ACAAATTGAT CCTGTACTAT TGAAGAACTC TAGCCAACAG 
GACAGCTCTT CCCGGGAAAG TCCCAGCTTA GAAGAAGAGG AGACTAAAAA GGAGGAAGAA 
ACAACTAAAC AAGGTAACAG CTGTCTTGAA TCCCTTCAAG TCTGTCAAGG AACATTGCTT 
CTGCATTGTG ATTTTGAAGA GGAACAGAAA GGAAGTGCAA AGATGAAAAG TGAAGAGCGG 
CCAATAGATT CAGAAAAATG TTCTACACCC AGTGCTCTAG AAGAGACTAC TGTGAAAATA 
GAAAAAGAAG ATGAAAAGGA ACTTGTAAAA CTGCCAGTCA TAGTGAAGCT AGAAAAACCT 
TTTCCAGAAA GTGAAGAAAA AAAGATTATC AAAGAAGAAA GTGATTCCTT CAAGGAAAAT 
GTCAAACCTG TAAAAGTGGA AATGAAAGAA TGTAAAGCAG ATCCTAGAGA TATCAAAGGT 
AGTATGGAGA AGTTAGAGCC TGAGAGGCTA GAGTTTGTTG GCAATGTTAA ATCTTCTCAA 
GAAATTACTG AAAAGTCTAC TGAAGAAACC GAGAAACTTA AAAATGACCA GCAGGCCAAG 
ATTCCACTAA AAAAACGAGA AATTAAACTG AGTGATGATT TTGATAGTCC AATCAAAGGA 
CCTTTGTGTA AATCAGTTAC TCCAACAAAA GAGTTTTTGA AAGATGAAAT AAAACAAGAG 
GAAGAGACTT GTAAAAGGAT CTCTACAATC ACTGCCTTGG GTCATGAAGG GAAACAGCTG 
GTAAATGGAG AAGTTAGTGA TGAAAAGGTA ACTCCACATT TTAAGACAGA ACAGATGGAG 
ACAAAGTTTT ATGATTCAAA GGAAGATAGT TGCAGCCCCT CTAAGGACAG AAGTGTCATC 
ATGGAGGGAA ATGGAGCAGA GTCTGTAAAT TCTGTCATTC CAAGTGTAAA AATAGGTGAT 
CTTGAGAAAG AAGTGGTCCC TTTAGGGAAA GATACAGATA ATTCAATATC AGTCTTAGAG 
ACCCCGAGTC AGAAAGAGTA CATAGACGAA ACTGGTCCTT CAGAAATGGA AACCTCTCTT 
GAGACTGCTG AAGTAGCAAA GGATCTCTGT TTAAAAACTG CTTTATCTGC CACTGAATCC 
TATAGTATGA GAGTTGAAGA GAAGTCTCCC AAAAGTAAGA AGGATAAGCG CCCACCAGTC 
CTAGAGTGTC TTGAAAAGTC CAAAAAGACT TTTCTTGATA AGGACATACA AAGATTGAGT 
CCAATACCGG AGGAGGTTCC AAAGACAACT GTAGAATCAG AGAAGGCTGG GTCTCCTGAG 
GCAGCTGAAA CTTATCCGTC GTCTAACGTG ACTGTCCACT GTGAGAAACT AGCCTCAGAA 
GTTGAGTGTC AGAATACAAG TTCACTAGAA GGTCATTCCC TGGAGAAAGT AGACCCAGAA 
ATTTTAAAAG TGGATTCTGA ATCAACAAAG GTAGAAGTGG ATAATCTGGA CAATGCCCAG 
ACCTCTGGCA CAGGTGATCC TTCTGAGACA AAGGGTTCTA TGCAAAAAAG CAAATTGAAA 
TATAAGTTGA TTCCTGAAGA AGAAAACACT GCCTCAGAAA ACACAGAGAT AACCTCCGAA 
AGGCAGAAAG AAGGCATCAA ATTAACAATT AGGATATCTA GTCGGAAAAA GAAGTCAGAT 
TCTCCTCCCA AAATTTTAGA ACCAGAAACC AAGCAAGAGA AGACAGAAAA GGAAGAAGAG 
AAAACAAACG TTGGTCGTAC TTTGAGGAGG TCTCCAAGAA TATCTAGACC CACAGCAAAA 
GTGGCTGAAA TCAGAGATCA GAAAGCTGAT AAAAAAAGAG GGGAAGAAGA TGAAGTAGAA 
GAAGAGTCAG CAGCTTTGCA AAAGACAGAC AAAAAAGAAA ATTTGAAAAA AGCAGAGAAG 
GATACAAATT CTAAAGTAGC CAAGGTAAAG CCCAAAGGCA AAGTTCGATG GACTGGCTCT 
CGAACACGTG GCAGGTGGAA ATATTCCAGC AATGATGAAA GTGAAGGGTC TGACAGTGAA 
AAATCATCTG CAGCTTCAGA AGAGGAGGAA GAAAAGGAAA GTGAAGAAGC CATCCTAGCA 
GATGATGATG AACCATGCAA AAAATGTGGC CTTCCAAACC ATCCTGAATT AATCCTTCTG 
TGTGACTCTT GTGACAGTGG ATACCACACT GCCTGCCTTC GCCCTCCTCT AATGATTATT 
CCAGATGGAG AATGGTTCTG CCCCCCTTGC CAGCATAAAC TACTGTGTGA AAAATTAGAA 
GAACAATTGC AAGATTTGGA TGTTGCCTTA AAGAAGAAAG AGCGTGCTGA ACGCAGGAAA 
GAACGCTTGG TGTATGTGGG TATTAGTATT GAAAACATCA TTCCTCCACA AGAGCCAGAT 
TTTTCGGAAG ATCATGAAGA AAAGAAAAAA GATTCAAAAA AATCCAAAGC AAACTTGCTT 
GAAAGGAGGT CGACAAGAAC ACGGAAATGT ATAAGTTACA GATTTGATGA GTTTGACGAA 
GCAATTGATG AAGCTATAGA AGATGATATC AAAGAGGCTG ATGGAGGAGG AGTTGGCCGA 
GGAAAAGATA TCTCCACCAT CACAGGCCAC CGTGGAAAAG ACATCTCTAC TATTTTGGAT 
GAAGAAAGAA AAGAAAATAA ACGACCCCAG AGGGCAGCTG CTGCTCGCCG GAAGAAACGC 
CGGCGACTGA ATGATCTGGA CAGTGACAGC AACCTGGATG AAGAGGAGAG CGAGGATGAA 
TTCAAGATCA GTGATGGATC TCAGGATGAA TTTGTTGTAT CTGATGAGAA CCCAGATGAG 
AGTGAAGAAG ACCCACCATC TAATGATGAC AGCGACACTG ATTTCTGTAG CCGAAGACTA 
AGGCGACACC CCTCCCGGCC TATGAGGCAA AGCAGGCGTT TGCGGAGAAA GACCCCAAAG 
AGAAAGTGCT CTGACGATGA TGAGGAGGAA GAGTCTGAGG AGAACAGCAG AGACTCTGAA 
AGTGACTTTA GTGATGACTT TAGCGATGAT TTTGTAGAAA CTCGGCGAAG AAGGTCTAGA 
AGGAACCAGA AGAGACAAAT TAACTACAAG GAAGATTCAG AAAGTGATGG CTCCCAGAAG 
AGTTTACGAC GTGGTAAAGA AATCAGACGA GTTCACAAGC GTAGGCTCTC TAGCTCAGAT 
AGTGAAGAGA GCTATATGTC CAAGAACTCT GAAGATGATG AGCTAGCTAA AGAATCAAAG 
CGGTCAGTTC GAAAACGGGG TCGAAGCACA GATGAGTATT CCGAAGCAGA TGAGGAGGAA 
GAAGAAGGTA AACCATCCCG AAAACGGCTA CACCGGATCG AGACAGATGA AGAGGAGAGT 
TGTGACAATG CTCATGGAGA TGCAGATCAA CCTGCCCATG ACCGCCAGCC CAGGGTCCTG 
CCCTCAGAAC AAGACAGCAC CAAAAAGCCC TATCGGATAG ACAGTGACGA GGAAGAGGAC 
TTTGAAAATG TAGGCAAGGT GGGGAGCCCA TTGGACTATA GCTTAGTGGA CCTACCTTCC 
ACCAATGGAC AGAGTCCTGG CAAAGCCATT GAAAACTTGA TTGGCAAGCC AGCTGAGAAG 
CCTCAGACCC CCAAGGACAG CAGCACAGCC AGTGCAAGCC TGGCCCCCAA TGGGACAAGT 
GGTGGGCAGG AGGCAGGGGC CCCAGAAGAG GACGAAGATG AGCTTTTAAG AGTGACTGAC 
CTTGTTGATT ATGTCTGTAA CAGTGAACAG TTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RSF1 ( XM_005226727.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005226784.1 Bos taurus remodeling and spacing factor 1 (RSF1), transcript variant X2, mRNA.

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CloneID	OBa12597
Clone ID Related Accession (Same CDS sequence)	XM_005197281.3 , XM_005226727.4 , XM_005226727.3
Accession Version	XM_005226727.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4356bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	remodeling and spacing factor 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104633.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005226727.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG CGGCGGCAGC GGCGGCGGCG ATGGCTCCTC CGGGCTGCCC GGGTTCGTGT 
CCCAACTTCG CCGTAGTCTG CTCTTTCTTG GAGCGCTACG GGCCGCTGCT CGACCTGCCT 
GAGTTGCCGT TCCCGGAGCT CGAGCGGGTG CTGCAAGCTC CGCCGCCGGA CGTCGGCAAC 
GGAGAAGTAC CTAAAGAATT GGTGGAGCTC CATTTGAAGT TGATGAGGAA GATTGGCAAA 
TCTGTTACAG CAGACAGATG GGAAAAATAC TTGATCAAGA TATGCCAAGA GTTTAATAGC 
ACCTGGGCGT GGGAGATGGA GAAGAAAGGC TATCTTGAAA TGAGTGTTGA ATGCAAGTTA 
GCACTCTTAA AGTACCTCTG TGAGTGTCAA TTTGATGACA ATCTCAAATT CAAGAATATT 
ATCAATGAGG AGGATGCCGA TACCATGCGT CTCCAGCCAA TTGGCCGTGA CAAAGATGGC 
CTCATGTACT GGTACCAGTT GGATCAAGAT CACAATGTCA GAATGTACAT AGAAGAACAA 
GATGATCAAG ATGGCTCTTC ATGGAAGTGC ATTGTGAGAA ATCGAAACGA GTTGGCTGAG 
ACACTTGCAC TCCTGAAAGC ACAAATTGAT CCTGTACTAT TGAAGAACTC TAGCCAACAG 
GACAGCTCTT CCCGGGAAAG TCCCAGCTTA GAAGAAGAGG AGACTAAAAA GGAGGAAGAA 
ACAACTAAAC AAGGTAACAG CTGTCTTGAA TCCCTTCAAG TCTGTCAAGG AACATTGCTT 
CTGCATTGTG ATTTTGAAGA GGAACAGAAA GGAAGTGCAA AGATGAAAAG TGAAGAGCGG 
CCAATAGATT CAGAAAAATG TTCTACACCC AGTGCTCTAG AAGAGACTAC TGTGAAAATA 
GAAAAAGAAG ATGAAAAGGA ACTTGTAAAA CTGCCAGTCA TAGTGAAGCT AGAAAAACCT 
TTTCCAGAAA GTGAAGAAAA AAAGATTATC AAAGAAGAAA GTGATTCCTT CAAGGAAAAT 
GTCAAACCTG TAAAAGTGGA AATGAAAGAA TGTAAAGCAG ATCCTAGAGA TATCAAAGGT 
AGTATGGAGA AGTTAGAGCC TGAGAGGCTA GAGTTTGTTG GCAATGTTAA ATCTTCTCAA 
GAAATTACTG AAAAGTCTAC TGAAGAAACC GAGAAACTTA AAAATGACCA GCAGGCCAAG 
ATTCCACTAA AAAAACGAGA AATTAAACTG AGTGATGATT TTGATAGTCC AATCAAAGGA 
CCTTTGTGTA AATCAGTTAC TCCAACAAAA GAGTTTTTGA AAGATGAAAT AAAACAAGAG 
GAAGAGACTT GTAAAAGGAT CTCTACAATC ACTGCCTTGG GTCATGAAGG GAAACAGCTG 
GTAAATGGAG AAGTTAGTGA TGAAAAGGTA ACTCCACATT TTAAGACAGA ACAGATGGAG 
ACAAAGTTTT ATGATTCAAA GGAAGATAGT TGCAGCCCCT CTAAGGACAG AAGTGTCATC 
ATGGAGGGAA ATGGAGCAGA GTCTGTAAAT TCTGTCATTC CAAGTGTAAA AATAGGTGAT 
CTTGAGAAAG AAGTGGTCCC TTTAGGGAAA GATACAGATA ATTCAATATC AGTCTTAGAG 
ACCCCGAGTC AGAAAGAGTA CATAGACGAA ACTGGTCCTT CAGAAATGGA AACCTCTCTT 
GAGACTGCTG AAGTAGCAAA GGATCTCTGT TTAAAAACTG CTTTATCTGC CACTGAATCC 
TATAGTATGA GAGTTGAAGA GAAGTCTCCC AAAAGTAAGA AGGATAAGCG CCCACCAGTC 
CTAGAGTGTC TTGAAAAGTC CAAAAAGACT TTTCTTGATA AGGACATACA AAGATTGAGT 
CCAATACCGG AGGAGGTTCC AAAGACAACT GTAGAATCAG AGAAGGCTGG GTCTCCTGAG 
GCAGCTGAAA CTTATCCGTC GTCTAACGTG ACTGTCCACT GTGAGAAACT AGCCTCAGAA 
GTTGAGTGTC AGAATACAAG TTCACTAGAA GGTCATTCCC TGGAGAAAGT AGACCCAGAA 
ATTTTAAAAG TGGATTCTGA ATCAACAAAG GTAGAAGTGG ATAATCTGGA CAATGCCCAG 
ACCTCTGGCA CAGGTGATCC TTCTGAGACA AAGGGTTCTA TGCAAAAAAG CAAATTGAAA 
TATAAGTTGA TTCCTGAAGA AGAAAACACT GCCTCAGAAA ACACAGAGAT AACCTCCGAA 
AGGCAGAAAG AAGGCATCAA ATTAACAATT AGGATATCTA GTCGGAAAAA GAAGTCAGAT 
TCTCCTCCCA AAATTTTAGA ACCAGAAACC AAGCAAGAGA AGACAGAAAA GGAAGAAGAG 
AAAACAAACG TTGGTCGTAC TTTGAGGAGG TCTCCAAGAA TATCTAGACC CACAGCAAAA 
GTGGCTGAAA TCAGAGATCA GAAAGCTGAT AAAAAAAGAG GGGAAGAAGA TGAAGTAGAA 
GAAGAGTCAG CAGCTTTGCA AAAGACAGAC AAAAAAGAAA ATTTGAAAAA AGCAGAGAAG 
GATACAAATT CTAAAGTAGC CAAGGTAAAG CCCAAAGGCA AAGTTCGATG GACTGGCTCT 
CGAACACGTG GCAGGTGGAA ATATTCCAGC AATGATGAAA GTGAAGGGTC TGACAGTGAA 
AAATCATCTG CAGCTTCAGA AGAGGAGGAA GAAAAGGAAA GTGAAGAAGC CATCCTAGCA 
GATGATGATG AACCATGCAA AAAATGTGGC CTTCCAAACC ATCCTGAATT AATCCTTCTG 
TGTGACTCTT GTGACAGTGG ATACCACACT GCCTGCCTTC GCCCTCCTCT AATGATTATT 
CCAGATGGAG AATGGTTCTG CCCCCCTTGC CAGCATAAAC TACTGTGTGA AAAATTAGAA 
GAACAATTGC AAGATTTGGA TGTTGCCTTA AAGAAGAAAG AGCGTGCTGA ACGCAGGAAA 
GAACGCTTGG TGTATGTGGG TATTAGTATT GAAAACATCA TTCCTCCACA AGAGCCAGAT 
TTTTCGGAAG ATCATGAAGA AAAGAAAAAA GATTCAAAAA AATCCAAAGC AAACTTGCTT 
GAAAGGAGGT CGACAAGAAC ACGGAAATGT ATAAGTTACA GATTTGATGA GTTTGACGAA 
GCAATTGATG AAGCTATAGA AGATGATATC AAAGAGGCTG ATGGAGGAGG AGTTGGCCGA 
GGAAAAGATA TCTCCACCAT CACAGGCCAC CGTGGAAAAG ACATCTCTAC TATTTTGGAT 
GAAGAAAGAA AAGAAAATAA ACGACCCCAG AGGGCAGCTG CTGCTCGCCG GAAGAAACGC 
CGGCGACTGA ATGATCTGGA CAGTGACAGC AACCTGGATG AAGAGGAGAG CGAGGATGAA 
TTCAAGATCA GTGATGGATC TCAGGATGAA TTTGTTGTAT CTGATGAGAA CCCAGATGAG 
AGTGAAGAAG ACCCACCATC TAATGATGAC AGCGACACTG ATTTCTGTAG CCGAAGACTA 
AGGCGACACC CCTCCCGGCC TATGAGGCAA AGCAGGCGTT TGCGGAGAAA GACCCCAAAG 
AGAAAGTGCT CTGACGATGA TGAGGAGGAA GAGTCTGAGG AGAACAGCAG AGACTCTGAA 
AGTGACTTTA GTGATGACTT TAGCGATGAT TTTGTAGAAA CTCGGCGAAG AAGGTCTAGA 
AGGAACCAGA AGAGACAAAT TAACTACAAG GAAGATTCAG AAAGTGATGG CTCCCAGAAG 
AGTTTACGAC GTGGTAAAGA AATCAGACGA GTTCACAAGC GTAGGCTCTC TAGCTCAGAT 
AGTGAAGAGA GCTATATGTC CAAGAACTCT GAAGATGATG AGCTAGCTAA AGAATCAAAG 
CGGTCAGTTC GAAAACGGGG TCGAAGCACA GATGAGTATT CCGAAGCAGA TGAGGAGGAA 
GAAGAAGGTA AACCATCCCG AAAACGGCTA CACCGGATCG AGACAGATGA AGAGGAGAGT 
TGTGACAATG CTCATGGAGA TGCAGATCAA CCTGCCCATG ACCGCCAGCC CAGGGTCCTG 
CCCTCAGAAC AAGACAGCAC CAAAAAGCCC TATCGGATAG ACAGTGACGA GGAAGAGGAC 
TTTGAAAATG TAGGCAAGGT GGGGAGCCCA TTGGACTATA GCTTAGTGGA CCTACCTTCC 
ACCAATGGAC AGAGTCCTGG CAAAGCCATT GAAAACTTGA TTGGCAAGCC AGCTGAGAAG 
CCTCAGACCC CCAAGGACAG CAGCACAGCC AGTGCAAGCC TGGCCCCCAA TGGGACAAGT 
GGTGGGCAGG AGGCAGGGGC CCCAGAAGAG GACGAAGATG AGCTTTTAAG AGTGACTGAC 
CTTGTTGATT ATGTCTGTAA CAGTGAACAG TTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005226784.1
CDS	284..4639
Translation	MAAAAAAAAAMAPPGCPGSCPNFAVVCSFLERYGPLLDLPELPFPELERVLQAPPPDVGNGEVPKELVELHLKLMRKIGKSVTADRWEKYLIKICQEFNSTWAWEMEKKGYLEMSVECKLALLKYLCECQFDDNLKFKNIINEEDADTMRLQPIGRDKDGLMYWYQLDQDHNVRMYIEEQDDQDGSSWKCIVRNRNELAETLALLKAQIDPVLLKNSSQQDSSSRESPSLEEEETKKEEETTKQGNSCLESLQVCQGTLLLHCDFEEEQKGSAKMKSEERPIDSEKCSTPSALEETTVKIEKEDEKELVKLPVIVKLEKPFPESEEKKIIKEESDSFKENVKPVKVEMKECKADPRDIKGSMEKLEPERLEFVGNVKSSQEITEKSTEETEKLKNDQQAKIPLKKREIKLSDDFDSPIKGPLCKSVTPTKEFLKDEIKQEEETCKRISTITALGHEGKQLVNGEVSDEKVTPHFKTEQMETKFYDSKEDSCSPSKDRSVIMEGNGAESVNSVIPSVKIGDLEKEVVPLGKDTDNSISVLETPSQKEYIDETGPSEMETSLETAEVAKDLCLKTALSATESYSMRVEEKSPKSKKDKRPPVLECLEKSKKTFLDKDIQRLSPIPEEVPKTTVESEKAGSPEAAETYPSSNVTVHCEKLASEVECQNTSSLEGHSLEKVDPEILKVDSESTKVEVDNLDNAQTSGTGDPSETKGSMQKSKLKYKLIPEEENTASENTEITSERQKEGIKLTIRISSRKKKSDSPPKILEPETKQEKTEKEEEKTNVGRTLRRSPRISRPTAKVAEIRDQKADKKRGEEDEVEEESAALQKTDKKENLKKAEKDTNSKVAKVKPKGKVRWTGSRTRGRWKYSSNDESEGSDSEKSSAASEEEEEKESEEAILADDDEPCKKCGLPNHPELILLCDSCDSGYHTACLRPPLMIIPDGEWFCPPCQHKLLCEKLEEQLQDLDVALKKKERAERRKERLVYVGISIENIIPPQEPDFSEDHEEKKKDSKKSKANLLERRSTRTRKCISYRFDEFDEAIDEAIEDDIKEADGGGVGRGKDISTITGHRGKDISTILDEERKENKRPQRAAAARRKKRRRLNDLDSDSNLDEEESEDEFKISDGSQDEFVVSDENPDESEEDPPSNDDSDTDFCSRRLRRHPSRPMRQSRRLRRKTPKRKCSDDDEEEESEENSRDSESDFSDDFSDDFVETRRRRSRRNQKRQINYKEDSESDGSQKSLRRGKEIRRVHKRRLSSSDSEESYMSKNSEDDELAKESKRSVRKRGRSTDEYSEADEEEEEGKPSRKRLHRIETDEEESCDNAHGDADQPAHDRQPRVLPSEQDSTKKPYRIDSDEEEDFENVGKVGSPLDYSLVDLPSTNGQSPGKAIENLIGKPAEKPQTPKDSSTASASLAPNGTSGGQEAGAPEEDEDELLRVTDLVDYVCNSEQL

Target ORF information:

RefSeq Version	XM_005226727.3
Organism	Bos taurus(cattle)
Definition	Bos taurus remodeling and spacing factor 1 (RSF1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005226727.3

ORF Insert Sequence:

ATGGCGGCGG CGGCGGCAGC GGCGGCGGCG ATGGCTCCTC CGGGCTGCCC GGGTTCGTGT 
CCCAACTTCG CCGTAGTCTG CTCTTTCTTG GAGCGCTACG GGCCGCTGCT CGACCTGCCT 
GAGTTGCCGT TCCCGGAGCT CGAGCGGGTG CTGCAAGCTC CGCCGCCGGA CGTCGGCAAC 
GGAGAAGTAC CTAAAGAATT GGTGGAGCTC CATTTGAAGT TGATGAGGAA GATTGGCAAA 
TCTGTTACAG CAGACAGATG GGAAAAATAC TTGATCAAGA TATGCCAAGA GTTTAATAGC 
ACCTGGGCGT GGGAGATGGA GAAGAAAGGC TATCTTGAAA TGAGTGTTGA ATGCAAGTTA 
GCACTCTTAA AGTACCTCTG TGAGTGTCAA TTTGATGACA ATCTCAAATT CAAGAATATT 
ATCAATGAGG AGGATGCCGA TACCATGCGT CTCCAGCCAA TTGGCCGTGA CAAAGATGGC 
CTCATGTACT GGTACCAGTT GGATCAAGAT CACAATGTCA GAATGTACAT AGAAGAACAA 
GATGATCAAG ATGGCTCTTC ATGGAAGTGC ATTGTGAGAA ATCGAAACGA GTTGGCTGAG 
ACACTTGCAC TCCTGAAAGC ACAAATTGAT CCTGTACTAT TGAAGAACTC TAGCCAACAG 
GACAGCTCTT CCCGGGAAAG TCCCAGCTTA GAAGAAGAGG AGACTAAAAA GGAGGAAGAA 
ACAACTAAAC AAGGTAACAG CTGTCTTGAA TCCCTTCAAG TCTGTCAAGG AACATTGCTT 
CTGCATTGTG ATTTTGAAGA GGAACAGAAA GGAAGTGCAA AGATGAAAAG TGAAGAGCGG 
CCAATAGATT CAGAAAAATG TTCTACACCC AGTGCTCTAG AAGAGACTAC TGTGAAAATA 
GAAAAAGAAG ATGAAAAGGA ACTTGTAAAA CTGCCAGTCA TAGTGAAGCT AGAAAAACCT 
TTTCCAGAAA GTGAAGAAAA AAAGATTATC AAAGAAGAAA GTGATTCCTT CAAGGAAAAT 
GTCAAACCTG TAAAAGTGGA AATGAAAGAA TGTAAAGCAG ATCCTAGAGA TATCAAAGGT 
AGTATGGAGA AGTTAGAGCC TGAGAGGCTA GAGTTTGTTG GCAATGTTAA ATCTTCTCAA 
GAAATTACTG AAAAGTCTAC TGAAGAAACC GAGAAACTTA AAAATGACCA GCAGGCCAAG 
ATTCCACTAA AAAAACGAGA AATTAAACTG AGTGATGATT TTGATAGTCC AATCAAAGGA 
CCTTTGTGTA AATCAGTTAC TCCAACAAAA GAGTTTTTGA AAGATGAAAT AAAACAAGAG 
GAAGAGACTT GTAAAAGGAT CTCTACAATC ACTGCCTTGG GTCATGAAGG GAAACAGCTG 
GTAAATGGAG AAGTTAGTGA TGAAAAGGTA ACTCCACATT TTAAGACAGA ACAGATGGAG 
ACAAAGTTTT ATGATTCAAA GGAAGATAGT TGCAGCCCCT CTAAGGACAG AAGTGTCATC 
ATGGAGGGAA ATGGAGCAGA GTCTGTAAAT TCTGTCATTC CAAGTGTAAA AATAGGTGAT 
CTTGAGAAAG AAGTGGTCCC TTTAGGGAAA GATACAGATA ATTCAATATC AGTCTTAGAG 
ACCCCGAGTC AGAAAGAGTA CATAGACGAA ACTGGTCCTT CAGAAATGGA AACCTCTCTT 
GAGACTGCTG AAGTAGCAAA GGATCTCTGT TTAAAAACTG CTTTATCTGC CACTGAATCC 
TATAGTATGA GAGTTGAAGA GAAGTCTCCC AAAAGTAAGA AGGATAAGCG CCCACCAGTC 
CTAGAGTGTC TTGAAAAGTC CAAAAAGACT TTTCTTGATA AGGACATACA AAGATTGAGT 
CCAATACCGG AGGAGGTTCC AAAGACAACT GTAGAATCAG AGAAGGCTGG GTCTCCTGAG 
GCAGCTGAAA CTTATCCGTC GTCTAACGTG ACTGTCCACT GTGAGAAACT AGCCTCAGAA 
GTTGAGTGTC AGAATACAAG TTCACTAGAA GGTCATTCCC TGGAGAAAGT AGACCCAGAA 
ATTTTAAAAG TGGATTCTGA ATCAACAAAG GTAGAAGTGG ATAATCTGGA CAATGCCCAG 
ACCTCTGGCA CAGGTGATCC TTCTGAGACA AAGGGTTCTA TGCAAAAAAG CAAATTGAAA 
TATAAGTTGA TTCCTGAAGA AGAAAACACT GCCTCAGAAA ACACAGAGAT AACCTCCGAA 
AGGCAGAAAG AAGGCATCAA ATTAACAATT AGGATATCTA GTCGGAAAAA GAAGTCAGAT 
TCTCCTCCCA AAATTTTAGA ACCAGAAACC AAGCAAGAGA AGACAGAAAA GGAAGAAGAG 
AAAACAAACG TTGGTCGTAC TTTGAGGAGG TCTCCAAGAA TATCTAGACC CACAGCAAAA 
GTGGCTGAAA TCAGAGATCA GAAAGCTGAT AAAAAAAGAG GGGAAGAAGA TGAAGTAGAA 
GAAGAGTCAG CAGCTTTGCA AAAGACAGAC AAAAAAGAAA ATTTGAAAAA AGCAGAGAAG 
GATACAAATT CTAAAGTAGC CAAGGTAAAG CCCAAAGGCA AAGTTCGATG GACTGGCTCT 
CGAACACGTG GCAGGTGGAA ATATTCCAGC AATGATGAAA GTGAAGGGTC TGACAGTGAA 
AAATCATCTG CAGCTTCAGA AGAGGAGGAA GAAAAGGAAA GTGAAGAAGC CATCCTAGCA 
GATGATGATG AACCATGCAA AAAATGTGGC CTTCCAAACC ATCCTGAATT AATCCTTCTG 
TGTGACTCTT GTGACAGTGG ATACCACACT GCCTGCCTTC GCCCTCCTCT AATGATTATT 
CCAGATGGAG AATGGTTCTG CCCCCCTTGC CAGCATAAAC TACTGTGTGA AAAATTAGAA 
GAACAATTGC AAGATTTGGA TGTTGCCTTA AAGAAGAAAG AGCGTGCTGA ACGCAGGAAA 
GAACGCTTGG TGTATGTGGG TATTAGTATT GAAAACATCA TTCCTCCACA AGAGCCAGAT 
TTTTCGGAAG ATCATGAAGA AAAGAAAAAA GATTCAAAAA AATCCAAAGC AAACTTGCTT 
GAAAGGAGGT CGACAAGAAC ACGGAAATGT ATAAGTTACA GATTTGATGA GTTTGACGAA 
GCAATTGATG AAGCTATAGA AGATGATATC AAAGAGGCTG ATGGAGGAGG AGTTGGCCGA 
GGAAAAGATA TCTCCACCAT CACAGGCCAC CGTGGAAAAG ACATCTCTAC TATTTTGGAT 
GAAGAAAGAA AAGAAAATAA ACGACCCCAG AGGGCAGCTG CTGCTCGCCG GAAGAAACGC 
CGGCGACTGA ATGATCTGGA CAGTGACAGC AACCTGGATG AAGAGGAGAG CGAGGATGAA 
TTCAAGATCA GTGATGGATC TCAGGATGAA TTTGTTGTAT CTGATGAGAA CCCAGATGAG 
AGTGAAGAAG ACCCACCATC TAATGATGAC AGCGACACTG ATTTCTGTAG CCGAAGACTA 
AGGCGACACC CCTCCCGGCC TATGAGGCAA AGCAGGCGTT TGCGGAGAAA GACCCCAAAG 
AGAAAGTGCT CTGACGATGA TGAGGAGGAA GAGTCTGAGG AGAACAGCAG AGACTCTGAA 
AGTGACTTTA GTGATGACTT TAGCGATGAT TTTGTAGAAA CTCGGCGAAG AAGGTCTAGA 
AGGAACCAGA AGAGACAAAT TAACTACAAG GAAGATTCAG AAAGTGATGG CTCCCAGAAG 
AGTTTACGAC GTGGTAAAGA AATCAGACGA GTTCACAAGC GTAGGCTCTC TAGCTCAGAT 
AGTGAAGAGA GCTATATGTC CAAGAACTCT GAAGATGATG AGCTAGCTAA AGAATCAAAG 
CGGTCAGTTC GAAAACGGGG TCGAAGCACA GATGAGTATT CCGAAGCAGA TGAGGAGGAA 
GAAGAAGGTA AACCATCCCG AAAACGGCTA CACCGGATCG AGACAGATGA AGAGGAGAGT 
TGTGACAATG CTCATGGAGA TGCAGATCAA CCTGCCCATG ACCGCCAGCC CAGGGTCCTG 
CCCTCAGAAC AAGACAGCAC CAAAAAGCCC TATCGGATAG ACAGTGACGA GGAAGAGGAC 
TTTGAAAATG TAGGCAAGGT GGGGAGCCCA TTGGACTATA GCTTAGTGGA CCTACCTTCC 
ACCAATGGAC AGAGTCCTGG CAAAGCCATT GAAAACTTGA TTGGCAAGCC AGCTGAGAAG 
CCTCAGACCC CCAAGGACAG CAGCACAGCC AGTGCAAGCC TGGCCCCCAA TGGGACAAGT 
GGTGGGCAGG AGGCAGGGGC CCCAGAAGAG GACGAAGATG AGCTTTTAAG AGTGACTGAC 
CTTGTTGATT ATGTCTGTAA CAGTGAACAG TTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RSF1 ( XM_002699063.5 ) cDNA ORF clone, Bos taurus(cattle) -> XP_002699109.1 Bos taurus remodeling and spacing factor 1 (RSF1), transcript variant X3, mRNA.

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CloneID	OBa12598
Clone ID Related Accession (Same CDS sequence)	XM_002699063.5 , XM_002699063.4 , XM_864539.7
Accession Version	XM_002699063.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4290bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	remodeling and spacing factor 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037356.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_002699063.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG CGGCGGCAGC GGCGGCGGCG ATGGCTCCTC CGGGCTGCCC GGGTTCGTGT 
CCCAACTTCG CCGTAGTCTG CTCTTTCTTG GAGCGCTACG GGCCGCTGCT CGACCTGCCT 
GAGTTGCCGT TCCCGGAGCT CGAGCGGGTG CTGCAAGCTC CGCCGCCGGA CGTCGGCAAC 
GGAGAAGTAC CTAAAGAATT GGTGGAGCTC CATTTGAAGT TGATGAGGAA GATTGGCAAA 
TCTGTTACAG CAGACAGATG GGAAAAATAC TTGATCAAGA TATGCCAAGA GTTTAATAGC 
ACCTGGGCGT GGGAGATGGA GAAGAAAGGC TATCTTGAAA TGAGTGTTGA ATGCAAGTTA 
GCACTCTTAA AGTACCTCTG TGAGTGTCAA TTTGATGACA ATCTCAAATT CAAGAATATT 
ATCAATGAGG AGGATGCCGA TACCATGCGT CTCCAGCCAA TTGGCCGTGA CAAAGATGGC 
CTCATGTACT GGTACCAGTT GGATCAAGAT CACAATGTCA GAATGTACAT AGAAGAACAA 
GATGATCAAG ATGGCTCTTC ATGGAAGTGC ATTGTGAGAA ATCGAAACGA GTTGGCTGAG 
ACACTTGCAC TCCTGAAAGC ACAAATTGAT CCTGTACTAT TGAAGAACTC TAGCCAACAG 
GACAGCTCTT CCCGGGAAAG TCCCAGCTTA GAAGAAGAGG AGACTAAAAA GGAGGAAGAA 
ACAACTAAAC AAGAGGAACA GAAAGGAAGT GCAAAGATGA AAAGTGAAGA GCGGCCAATA 
GATTCAGAAA AATGTTCTAC ACCCAGTGCT CTAGAAGAGA CTACTGTGAA AATAGAAAAA 
GAAGATGAAA AGGAACTTGT AAAACTGCCA GTCATAGTGA AGCTAGAAAA ACCTTTTCCA 
GAAAGTGAAG AAAAAAAGAT TATCAAAGAA GAAAGTGATT CCTTCAAGGA AAATGTCAAA 
CCTGTAAAAG TGGAAATGAA AGAATGTAAA GCAGATCCTA GAGATATCAA AGGTAGTATG 
GAGAAGTTAG AGCCTGAGAG GCTAGAGTTT GTTGGCAATG TTAAATCTTC TCAAGAAATT 
ACTGAAAAGT CTACTGAAGA AACCGAGAAA CTTAAAAATG ACCAGCAGGC CAAGATTCCA 
CTAAAAAAAC GAGAAATTAA ACTGAGTGAT GATTTTGATA GTCCAATCAA AGGACCTTTG 
TGTAAATCAG TTACTCCAAC AAAAGAGTTT TTGAAAGATG AAATAAAACA AGAGGAAGAG 
ACTTGTAAAA GGATCTCTAC AATCACTGCC TTGGGTCATG AAGGGAAACA GCTGGTAAAT 
GGAGAAGTTA GTGATGAAAA GGTAACTCCA CATTTTAAGA CAGAACAGAT GGAGACAAAG 
TTTTATGATT CAAAGGAAGA TAGTTGCAGC CCCTCTAAGG ACAGAAGTGT CATCATGGAG 
GGAAATGGAG CAGAGTCTGT AAATTCTGTC ATTCCAAGTG TAAAAATAGG TGATCTTGAG 
AAAGAAGTGG TCCCTTTAGG GAAAGATACA GATAATTCAA TATCAGTCTT AGAGACCCCG 
AGTCAGAAAG AGTACATAGA CGAAACTGGT CCTTCAGAAA TGGAAACCTC TCTTGAGACT 
GCTGAAGTAG CAAAGGATCT CTGTTTAAAA ACTGCTTTAT CTGCCACTGA ATCCTATAGT 
ATGAGAGTTG AAGAGAAGTC TCCCAAAAGT AAGAAGGATA AGCGCCCACC AGTCCTAGAG 
TGTCTTGAAA AGTCCAAAAA GACTTTTCTT GATAAGGACA TACAAAGATT GAGTCCAATA 
CCGGAGGAGG TTCCAAAGAC AACTGTAGAA TCAGAGAAGG CTGGGTCTCC TGAGGCAGCT 
GAAACTTATC CGTCGTCTAA CGTGACTGTC CACTGTGAGA AACTAGCCTC AGAAGTTGAG 
TGTCAGAATA CAAGTTCACT AGAAGGTCAT TCCCTGGAGA AAGTAGACCC AGAAATTTTA 
AAAGTGGATT CTGAATCAAC AAAGGTAGAA GTGGATAATC TGGACAATGC CCAGACCTCT 
GGCACAGGTG ATCCTTCTGA GACAAAGGGT TCTATGCAAA AAAGCAAATT GAAATATAAG 
TTGATTCCTG AAGAAGAAAA CACTGCCTCA GAAAACACAG AGATAACCTC CGAAAGGCAG 
AAAGAAGGCA TCAAATTAAC AATTAGGATA TCTAGTCGGA AAAAGAAGTC AGATTCTCCT 
CCCAAAATTT TAGAACCAGA AACCAAGCAA GAGAAGACAG AAAAGGAAGA AGAGAAAACA 
AACGTTGGTC GTACTTTGAG GAGGTCTCCA AGAATATCTA GACCCACAGC AAAAGTGGCT 
GAAATCAGAG ATCAGAAAGC TGATAAAAAA AGAGGGGAAG AAGATGAAGT AGAAGAAGAG 
TCAGCAGCTT TGCAAAAGAC AGACAAAAAA GAAAATTTGA AAAAAGCAGA GAAGGATACA 
AATTCTAAAG TAGCCAAGGT AAAGCCCAAA GGCAAAGTTC GATGGACTGG CTCTCGAACA 
CGTGGCAGGT GGAAATATTC CAGCAATGAT GAAAGTGAAG GGTCTGACAG TGAAAAATCA 
TCTGCAGCTT CAGAAGAGGA GGAAGAAAAG GAAAGTGAAG AAGCCATCCT AGCAGATGAT 
GATGAACCAT GCAAAAAATG TGGCCTTCCA AACCATCCTG AATTAATCCT TCTGTGTGAC 
TCTTGTGACA GTGGATACCA CACTGCCTGC CTTCGCCCTC CTCTAATGAT TATTCCAGAT 
GGAGAATGGT TCTGCCCCCC TTGCCAGCAT AAACTACTGT GTGAAAAATT AGAAGAACAA 
TTGCAAGATT TGGATGTTGC CTTAAAGAAG AAAGAGCGTG CTGAACGCAG GAAAGAACGC 
TTGGTGTATG TGGGTATTAG TATTGAAAAC ATCATTCCTC CACAAGAGCC AGATTTTTCG 
GAAGATCATG AAGAAAAGAA AAAAGATTCA AAAAAATCCA AAGCAAACTT GCTTGAAAGG 
AGGTCGACAA GAACACGGAA ATGTATAAGT TACAGATTTG ATGAGTTTGA CGAAGCAATT 
GATGAAGCTA TAGAAGATGA TATCAAAGAG GCTGATGGAG GAGGAGTTGG CCGAGGAAAA 
GATATCTCCA CCATCACAGG CCACCGTGGA AAAGACATCT CTACTATTTT GGATGAAGAA 
AGAAAAGAAA ATAAACGACC CCAGAGGGCA GCTGCTGCTC GCCGGAAGAA ACGCCGGCGA 
CTGAATGATC TGGACAGTGA CAGCAACCTG GATGAAGAGG AGAGCGAGGA TGAATTCAAG 
ATCAGTGATG GATCTCAGGA TGAATTTGTT GTATCTGATG AGAACCCAGA TGAGAGTGAA 
GAAGACCCAC CATCTAATGA TGACAGCGAC ACTGATTTCT GTAGCCGAAG ACTAAGGCGA 
CACCCCTCCC GGCCTATGAG GCAAAGCAGG CGTTTGCGGA GAAAGACCCC AAAGAGAAAG 
TGCTCTGACG ATGATGAGGA GGAAGAGTCT GAGGAGAACA GCAGAGACTC TGAAAGTGAC 
TTTAGTGATG ACTTTAGCGA TGATTTTGTA GAAACTCGGC GAAGAAGGTC TAGAAGGAAC 
CAGAAGAGAC AAATTAACTA CAAGGAAGAT TCAGAAAGTG ATGGCTCCCA GAAGAGTTTA 
CGACGTGGTA AAGAAATCAG ACGAGTTCAC AAGCGTAGGC TCTCTAGCTC AGATAGTGAA 
GAGAGCTATA TGTCCAAGAA CTCTGAAGAT GATGAGCTAG CTAAAGAATC AAAGCGGTCA 
GTTCGAAAAC GGGGTCGAAG CACAGATGAG TATTCCGAAG CAGATGAGGA GGAAGAAGAA 
GGTAAACCAT CCCGAAAACG GCTACACCGG ATCGAGACAG ATGAAGAGGA GAGTTGTGAC 
AATGCTCATG GAGATGCAGA TCAACCTGCC CATGACCGCC AGCCCAGGGT CCTGCCCTCA 
GAACAAGACA GCACCAAAAA GCCCTATCGG ATAGACAGTG ACGAGGAAGA GGACTTTGAA 
AATGTAGGCA AGGTGGGGAG CCCATTGGAC TATAGCTTAG TGGACCTACC TTCCACCAAT 
GGACAGAGTC CTGGCAAAGC CATTGAAAAC TTGATTGGCA AGCCAGCTGA GAAGCCTCAG 
ACCCCCAAGG ACAGCAGCAC AGCCAGTGCA AGCCTGGCCC CCAATGGGAC AAGTGGTGGG 
CAGGAGGCAG GGGCCCCAGA AGAGGACGAA GATGAGCTTT TAAGAGTGAC TGACCTTGTT 
GATTATGTCT GTAACAGTGA ACAGTTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002699109.1
CDS	280..4569
Translation	MAAAAAAAAAMAPPGCPGSCPNFAVVCSFLERYGPLLDLPELPFPELERVLQAPPPDVGNGEVPKELVELHLKLMRKIGKSVTADRWEKYLIKICQEFNSTWAWEMEKKGYLEMSVECKLALLKYLCECQFDDNLKFKNIINEEDADTMRLQPIGRDKDGLMYWYQLDQDHNVRMYIEEQDDQDGSSWKCIVRNRNELAETLALLKAQIDPVLLKNSSQQDSSSRESPSLEEEETKKEEETTKQEEQKGSAKMKSEERPIDSEKCSTPSALEETTVKIEKEDEKELVKLPVIVKLEKPFPESEEKKIIKEESDSFKENVKPVKVEMKECKADPRDIKGSMEKLEPERLEFVGNVKSSQEITEKSTEETEKLKNDQQAKIPLKKREIKLSDDFDSPIKGPLCKSVTPTKEFLKDEIKQEEETCKRISTITALGHEGKQLVNGEVSDEKVTPHFKTEQMETKFYDSKEDSCSPSKDRSVIMEGNGAESVNSVIPSVKIGDLEKEVVPLGKDTDNSISVLETPSQKEYIDETGPSEMETSLETAEVAKDLCLKTALSATESYSMRVEEKSPKSKKDKRPPVLECLEKSKKTFLDKDIQRLSPIPEEVPKTTVESEKAGSPEAAETYPSSNVTVHCEKLASEVECQNTSSLEGHSLEKVDPEILKVDSESTKVEVDNLDNAQTSGTGDPSETKGSMQKSKLKYKLIPEEENTASENTEITSERQKEGIKLTIRISSRKKKSDSPPKILEPETKQEKTEKEEEKTNVGRTLRRSPRISRPTAKVAEIRDQKADKKRGEEDEVEEESAALQKTDKKENLKKAEKDTNSKVAKVKPKGKVRWTGSRTRGRWKYSSNDESEGSDSEKSSAASEEEEEKESEEAILADDDEPCKKCGLPNHPELILLCDSCDSGYHTACLRPPLMIIPDGEWFCPPCQHKLLCEKLEEQLQDLDVALKKKERAERRKERLVYVGISIENIIPPQEPDFSEDHEEKKKDSKKSKANLLERRSTRTRKCISYRFDEFDEAIDEAIEDDIKEADGGGVGRGKDISTITGHRGKDISTILDEERKENKRPQRAAAARRKKRRRLNDLDSDSNLDEEESEDEFKISDGSQDEFVVSDENPDESEEDPPSNDDSDTDFCSRRLRRHPSRPMRQSRRLRRKTPKRKCSDDDEEEESEENSRDSESDFSDDFSDDFVETRRRRSRRNQKRQINYKEDSESDGSQKSLRRGKEIRRVHKRRLSSSDSEESYMSKNSEDDELAKESKRSVRKRGRSTDEYSEADEEEEEGKPSRKRLHRIETDEEESCDNAHGDADQPAHDRQPRVLPSEQDSTKKPYRIDSDEEEDFENVGKVGSPLDYSLVDLPSTNGQSPGKAIENLIGKPAEKPQTPKDSSTASASLAPNGTSGGQEAGAPEEDEDELLRVTDLVDYVCNSEQL

Target ORF information:

RefSeq Version	XM_002699063.5
Organism	Bos taurus(cattle)
Definition	Bos taurus remodeling and spacing factor 1 (RSF1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002699063.5

ORF Insert Sequence:

ATGGCGGCGG CGGCGGCAGC GGCGGCGGCG ATGGCTCCTC CGGGCTGCCC GGGTTCGTGT 
CCCAACTTCG CCGTAGTCTG CTCTTTCTTG GAGCGCTACG GGCCGCTGCT CGACCTGCCT 
GAGTTGCCGT TCCCGGAGCT CGAGCGGGTG CTGCAAGCTC CGCCGCCGGA CGTCGGCAAC 
GGAGAAGTAC CTAAAGAATT GGTGGAGCTC CATTTGAAGT TGATGAGGAA GATTGGCAAA 
TCTGTTACAG CAGACAGATG GGAAAAATAC TTGATCAAGA TATGCCAAGA GTTTAATAGC 
ACCTGGGCGT GGGAGATGGA GAAGAAAGGC TATCTTGAAA TGAGTGTTGA ATGCAAGTTA 
GCACTCTTAA AGTACCTCTG TGAGTGTCAA TTTGATGACA ATCTCAAATT CAAGAATATT 
ATCAATGAGG AGGATGCCGA TACCATGCGT CTCCAGCCAA TTGGCCGTGA CAAAGATGGC 
CTCATGTACT GGTACCAGTT GGATCAAGAT CACAATGTCA GAATGTACAT AGAAGAACAA 
GATGATCAAG ATGGCTCTTC ATGGAAGTGC ATTGTGAGAA ATCGAAACGA GTTGGCTGAG 
ACACTTGCAC TCCTGAAAGC ACAAATTGAT CCTGTACTAT TGAAGAACTC TAGCCAACAG 
GACAGCTCTT CCCGGGAAAG TCCCAGCTTA GAAGAAGAGG AGACTAAAAA GGAGGAAGAA 
ACAACTAAAC AAGAGGAACA GAAAGGAAGT GCAAAGATGA AAAGTGAAGA GCGGCCAATA 
GATTCAGAAA AATGTTCTAC ACCCAGTGCT CTAGAAGAGA CTACTGTGAA AATAGAAAAA 
GAAGATGAAA AGGAACTTGT AAAACTGCCA GTCATAGTGA AGCTAGAAAA ACCTTTTCCA 
GAAAGTGAAG AAAAAAAGAT TATCAAAGAA GAAAGTGATT CCTTCAAGGA AAATGTCAAA 
CCTGTAAAAG TGGAAATGAA AGAATGTAAA GCAGATCCTA GAGATATCAA AGGTAGTATG 
GAGAAGTTAG AGCCTGAGAG GCTAGAGTTT GTTGGCAATG TTAAATCTTC TCAAGAAATT 
ACTGAAAAGT CTACTGAAGA AACCGAGAAA CTTAAAAATG ACCAGCAGGC CAAGATTCCA 
CTAAAAAAAC GAGAAATTAA ACTGAGTGAT GATTTTGATA GTCCAATCAA AGGACCTTTG 
TGTAAATCAG TTACTCCAAC AAAAGAGTTT TTGAAAGATG AAATAAAACA AGAGGAAGAG 
ACTTGTAAAA GGATCTCTAC AATCACTGCC TTGGGTCATG AAGGGAAACA GCTGGTAAAT 
GGAGAAGTTA GTGATGAAAA GGTAACTCCA CATTTTAAGA CAGAACAGAT GGAGACAAAG 
TTTTATGATT CAAAGGAAGA TAGTTGCAGC CCCTCTAAGG ACAGAAGTGT CATCATGGAG 
GGAAATGGAG CAGAGTCTGT AAATTCTGTC ATTCCAAGTG TAAAAATAGG TGATCTTGAG 
AAAGAAGTGG TCCCTTTAGG GAAAGATACA GATAATTCAA TATCAGTCTT AGAGACCCCG 
AGTCAGAAAG AGTACATAGA CGAAACTGGT CCTTCAGAAA TGGAAACCTC TCTTGAGACT 
GCTGAAGTAG CAAAGGATCT CTGTTTAAAA ACTGCTTTAT CTGCCACTGA ATCCTATAGT 
ATGAGAGTTG AAGAGAAGTC TCCCAAAAGT AAGAAGGATA AGCGCCCACC AGTCCTAGAG 
TGTCTTGAAA AGTCCAAAAA GACTTTTCTT GATAAGGACA TACAAAGATT GAGTCCAATA 
CCGGAGGAGG TTCCAAAGAC AACTGTAGAA TCAGAGAAGG CTGGGTCTCC TGAGGCAGCT 
GAAACTTATC CGTCGTCTAA CGTGACTGTC CACTGTGAGA AACTAGCCTC AGAAGTTGAG 
TGTCAGAATA CAAGTTCACT AGAAGGTCAT TCCCTGGAGA AAGTAGACCC AGAAATTTTA 
AAAGTGGATT CTGAATCAAC AAAGGTAGAA GTGGATAATC TGGACAATGC CCAGACCTCT 
GGCACAGGTG ATCCTTCTGA GACAAAGGGT TCTATGCAAA AAAGCAAATT GAAATATAAG 
TTGATTCCTG AAGAAGAAAA CACTGCCTCA GAAAACACAG AGATAACCTC CGAAAGGCAG 
AAAGAAGGCA TCAAATTAAC AATTAGGATA TCTAGTCGGA AAAAGAAGTC AGATTCTCCT 
CCCAAAATTT TAGAACCAGA AACCAAGCAA GAGAAGACAG AAAAGGAAGA AGAGAAAACA 
AACGTTGGTC GTACTTTGAG GAGGTCTCCA AGAATATCTA GACCCACAGC AAAAGTGGCT 
GAAATCAGAG ATCAGAAAGC TGATAAAAAA AGAGGGGAAG AAGATGAAGT AGAAGAAGAG 
TCAGCAGCTT TGCAAAAGAC AGACAAAAAA GAAAATTTGA AAAAAGCAGA GAAGGATACA 
AATTCTAAAG TAGCCAAGGT AAAGCCCAAA GGCAAAGTTC GATGGACTGG CTCTCGAACA 
CGTGGCAGGT GGAAATATTC CAGCAATGAT GAAAGTGAAG GGTCTGACAG TGAAAAATCA 
TCTGCAGCTT CAGAAGAGGA GGAAGAAAAG GAAAGTGAAG AAGCCATCCT AGCAGATGAT 
GATGAACCAT GCAAAAAATG TGGCCTTCCA AACCATCCTG AATTAATCCT TCTGTGTGAC 
TCTTGTGACA GTGGATACCA CACTGCCTGC CTTCGCCCTC CTCTAATGAT TATTCCAGAT 
GGAGAATGGT TCTGCCCCCC TTGCCAGCAT AAACTACTGT GTGAAAAATT AGAAGAACAA 
TTGCAAGATT TGGATGTTGC CTTAAAGAAG AAAGAGCGTG CTGAACGCAG GAAAGAACGC 
TTGGTGTATG TGGGTATTAG TATTGAAAAC ATCATTCCTC CACAAGAGCC AGATTTTTCG 
GAAGATCATG AAGAAAAGAA AAAAGATTCA AAAAAATCCA AAGCAAACTT GCTTGAAAGG 
AGGTCGACAA GAACACGGAA ATGTATAAGT TACAGATTTG ATGAGTTTGA CGAAGCAATT 
GATGAAGCTA TAGAAGATGA TATCAAAGAG GCTGATGGAG GAGGAGTTGG CCGAGGAAAA 
GATATCTCCA CCATCACAGG CCACCGTGGA AAAGACATCT CTACTATTTT GGATGAAGAA 
AGAAAAGAAA ATAAACGACC CCAGAGGGCA GCTGCTGCTC GCCGGAAGAA ACGCCGGCGA 
CTGAATGATC TGGACAGTGA CAGCAACCTG GATGAAGAGG AGAGCGAGGA TGAATTCAAG 
ATCAGTGATG GATCTCAGGA TGAATTTGTT GTATCTGATG AGAACCCAGA TGAGAGTGAA 
GAAGACCCAC CATCTAATGA TGACAGCGAC ACTGATTTCT GTAGCCGAAG ACTAAGGCGA 
CACCCCTCCC GGCCTATGAG GCAAAGCAGG CGTTTGCGGA GAAAGACCCC AAAGAGAAAG 
TGCTCTGACG ATGATGAGGA GGAAGAGTCT GAGGAGAACA GCAGAGACTC TGAAAGTGAC 
TTTAGTGATG ACTTTAGCGA TGATTTTGTA GAAACTCGGC GAAGAAGGTC TAGAAGGAAC 
CAGAAGAGAC AAATTAACTA CAAGGAAGAT TCAGAAAGTG ATGGCTCCCA GAAGAGTTTA 
CGACGTGGTA AAGAAATCAG ACGAGTTCAC AAGCGTAGGC TCTCTAGCTC AGATAGTGAA 
GAGAGCTATA TGTCCAAGAA CTCTGAAGAT GATGAGCTAG CTAAAGAATC AAAGCGGTCA 
GTTCGAAAAC GGGGTCGAAG CACAGATGAG TATTCCGAAG CAGATGAGGA GGAAGAAGAA 
GGTAAACCAT CCCGAAAACG GCTACACCGG ATCGAGACAG ATGAAGAGGA GAGTTGTGAC 
AATGCTCATG GAGATGCAGA TCAACCTGCC CATGACCGCC AGCCCAGGGT CCTGCCCTCA 
GAACAAGACA GCACCAAAAA GCCCTATCGG ATAGACAGTG ACGAGGAAGA GGACTTTGAA 
AATGTAGGCA AGGTGGGGAG CCCATTGGAC TATAGCTTAG TGGACCTACC TTCCACCAAT 
GGACAGAGTC CTGGCAAAGC CATTGAAAAC TTGATTGGCA AGCCAGCTGA GAAGCCTCAG 
ACCCCCAAGG ACAGCAGCAC AGCCAGTGCA AGCCTGGCCC CCAATGGGAC AAGTGGTGGG 
CAGGAGGCAG GGGCCCCAGA AGAGGACGAA GATGAGCTTT TAAGAGTGAC TGACCTTGTT 
GATTATGTCT GTAACAGTGA ACAGTTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RSF1 ( XM_002699063.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_002699109.1 Bos taurus remodeling and spacing factor 1 (RSF1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa12598
Clone ID Related Accession (Same CDS sequence)	XM_002699063.5 , XM_002699063.4 , XM_864539.7
Accession Version	XM_002699063.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4290bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	remodeling and spacing factor 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104633.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_002699063.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG CGGCGGCAGC GGCGGCGGCG ATGGCTCCTC CGGGCTGCCC GGGTTCGTGT 
CCCAACTTCG CCGTAGTCTG CTCTTTCTTG GAGCGCTACG GGCCGCTGCT CGACCTGCCT 
GAGTTGCCGT TCCCGGAGCT CGAGCGGGTG CTGCAAGCTC CGCCGCCGGA CGTCGGCAAC 
GGAGAAGTAC CTAAAGAATT GGTGGAGCTC CATTTGAAGT TGATGAGGAA GATTGGCAAA 
TCTGTTACAG CAGACAGATG GGAAAAATAC TTGATCAAGA TATGCCAAGA GTTTAATAGC 
ACCTGGGCGT GGGAGATGGA GAAGAAAGGC TATCTTGAAA TGAGTGTTGA ATGCAAGTTA 
GCACTCTTAA AGTACCTCTG TGAGTGTCAA TTTGATGACA ATCTCAAATT CAAGAATATT 
ATCAATGAGG AGGATGCCGA TACCATGCGT CTCCAGCCAA TTGGCCGTGA CAAAGATGGC 
CTCATGTACT GGTACCAGTT GGATCAAGAT CACAATGTCA GAATGTACAT AGAAGAACAA 
GATGATCAAG ATGGCTCTTC ATGGAAGTGC ATTGTGAGAA ATCGAAACGA GTTGGCTGAG 
ACACTTGCAC TCCTGAAAGC ACAAATTGAT CCTGTACTAT TGAAGAACTC TAGCCAACAG 
GACAGCTCTT CCCGGGAAAG TCCCAGCTTA GAAGAAGAGG AGACTAAAAA GGAGGAAGAA 
ACAACTAAAC AAGAGGAACA GAAAGGAAGT GCAAAGATGA AAAGTGAAGA GCGGCCAATA 
GATTCAGAAA AATGTTCTAC ACCCAGTGCT CTAGAAGAGA CTACTGTGAA AATAGAAAAA 
GAAGATGAAA AGGAACTTGT AAAACTGCCA GTCATAGTGA AGCTAGAAAA ACCTTTTCCA 
GAAAGTGAAG AAAAAAAGAT TATCAAAGAA GAAAGTGATT CCTTCAAGGA AAATGTCAAA 
CCTGTAAAAG TGGAAATGAA AGAATGTAAA GCAGATCCTA GAGATATCAA AGGTAGTATG 
GAGAAGTTAG AGCCTGAGAG GCTAGAGTTT GTTGGCAATG TTAAATCTTC TCAAGAAATT 
ACTGAAAAGT CTACTGAAGA AACCGAGAAA CTTAAAAATG ACCAGCAGGC CAAGATTCCA 
CTAAAAAAAC GAGAAATTAA ACTGAGTGAT GATTTTGATA GTCCAATCAA AGGACCTTTG 
TGTAAATCAG TTACTCCAAC AAAAGAGTTT TTGAAAGATG AAATAAAACA AGAGGAAGAG 
ACTTGTAAAA GGATCTCTAC AATCACTGCC TTGGGTCATG AAGGGAAACA GCTGGTAAAT 
GGAGAAGTTA GTGATGAAAA GGTAACTCCA CATTTTAAGA CAGAACAGAT GGAGACAAAG 
TTTTATGATT CAAAGGAAGA TAGTTGCAGC CCCTCTAAGG ACAGAAGTGT CATCATGGAG 
GGAAATGGAG CAGAGTCTGT AAATTCTGTC ATTCCAAGTG TAAAAATAGG TGATCTTGAG 
AAAGAAGTGG TCCCTTTAGG GAAAGATACA GATAATTCAA TATCAGTCTT AGAGACCCCG 
AGTCAGAAAG AGTACATAGA CGAAACTGGT CCTTCAGAAA TGGAAACCTC TCTTGAGACT 
GCTGAAGTAG CAAAGGATCT CTGTTTAAAA ACTGCTTTAT CTGCCACTGA ATCCTATAGT 
ATGAGAGTTG AAGAGAAGTC TCCCAAAAGT AAGAAGGATA AGCGCCCACC AGTCCTAGAG 
TGTCTTGAAA AGTCCAAAAA GACTTTTCTT GATAAGGACA TACAAAGATT GAGTCCAATA 
CCGGAGGAGG TTCCAAAGAC AACTGTAGAA TCAGAGAAGG CTGGGTCTCC TGAGGCAGCT 
GAAACTTATC CGTCGTCTAA CGTGACTGTC CACTGTGAGA AACTAGCCTC AGAAGTTGAG 
TGTCAGAATA CAAGTTCACT AGAAGGTCAT TCCCTGGAGA AAGTAGACCC AGAAATTTTA 
AAAGTGGATT CTGAATCAAC AAAGGTAGAA GTGGATAATC TGGACAATGC CCAGACCTCT 
GGCACAGGTG ATCCTTCTGA GACAAAGGGT TCTATGCAAA AAAGCAAATT GAAATATAAG 
TTGATTCCTG AAGAAGAAAA CACTGCCTCA GAAAACACAG AGATAACCTC CGAAAGGCAG 
AAAGAAGGCA TCAAATTAAC AATTAGGATA TCTAGTCGGA AAAAGAAGTC AGATTCTCCT 
CCCAAAATTT TAGAACCAGA AACCAAGCAA GAGAAGACAG AAAAGGAAGA AGAGAAAACA 
AACGTTGGTC GTACTTTGAG GAGGTCTCCA AGAATATCTA GACCCACAGC AAAAGTGGCT 
GAAATCAGAG ATCAGAAAGC TGATAAAAAA AGAGGGGAAG AAGATGAAGT AGAAGAAGAG 
TCAGCAGCTT TGCAAAAGAC AGACAAAAAA GAAAATTTGA AAAAAGCAGA GAAGGATACA 
AATTCTAAAG TAGCCAAGGT AAAGCCCAAA GGCAAAGTTC GATGGACTGG CTCTCGAACA 
CGTGGCAGGT GGAAATATTC CAGCAATGAT GAAAGTGAAG GGTCTGACAG TGAAAAATCA 
TCTGCAGCTT CAGAAGAGGA GGAAGAAAAG GAAAGTGAAG AAGCCATCCT AGCAGATGAT 
GATGAACCAT GCAAAAAATG TGGCCTTCCA AACCATCCTG AATTAATCCT TCTGTGTGAC 
TCTTGTGACA GTGGATACCA CACTGCCTGC CTTCGCCCTC CTCTAATGAT TATTCCAGAT 
GGAGAATGGT TCTGCCCCCC TTGCCAGCAT AAACTACTGT GTGAAAAATT AGAAGAACAA 
TTGCAAGATT TGGATGTTGC CTTAAAGAAG AAAGAGCGTG CTGAACGCAG GAAAGAACGC 
TTGGTGTATG TGGGTATTAG TATTGAAAAC ATCATTCCTC CACAAGAGCC AGATTTTTCG 
GAAGATCATG AAGAAAAGAA AAAAGATTCA AAAAAATCCA AAGCAAACTT GCTTGAAAGG 
AGGTCGACAA GAACACGGAA ATGTATAAGT TACAGATTTG ATGAGTTTGA CGAAGCAATT 
GATGAAGCTA TAGAAGATGA TATCAAAGAG GCTGATGGAG GAGGAGTTGG CCGAGGAAAA 
GATATCTCCA CCATCACAGG CCACCGTGGA AAAGACATCT CTACTATTTT GGATGAAGAA 
AGAAAAGAAA ATAAACGACC CCAGAGGGCA GCTGCTGCTC GCCGGAAGAA ACGCCGGCGA 
CTGAATGATC TGGACAGTGA CAGCAACCTG GATGAAGAGG AGAGCGAGGA TGAATTCAAG 
ATCAGTGATG GATCTCAGGA TGAATTTGTT GTATCTGATG AGAACCCAGA TGAGAGTGAA 
GAAGACCCAC CATCTAATGA TGACAGCGAC ACTGATTTCT GTAGCCGAAG ACTAAGGCGA 
CACCCCTCCC GGCCTATGAG GCAAAGCAGG CGTTTGCGGA GAAAGACCCC AAAGAGAAAG 
TGCTCTGACG ATGATGAGGA GGAAGAGTCT GAGGAGAACA GCAGAGACTC TGAAAGTGAC 
TTTAGTGATG ACTTTAGCGA TGATTTTGTA GAAACTCGGC GAAGAAGGTC TAGAAGGAAC 
CAGAAGAGAC AAATTAACTA CAAGGAAGAT TCAGAAAGTG ATGGCTCCCA GAAGAGTTTA 
CGACGTGGTA AAGAAATCAG ACGAGTTCAC AAGCGTAGGC TCTCTAGCTC AGATAGTGAA 
GAGAGCTATA TGTCCAAGAA CTCTGAAGAT GATGAGCTAG CTAAAGAATC AAAGCGGTCA 
GTTCGAAAAC GGGGTCGAAG CACAGATGAG TATTCCGAAG CAGATGAGGA GGAAGAAGAA 
GGTAAACCAT CCCGAAAACG GCTACACCGG ATCGAGACAG ATGAAGAGGA GAGTTGTGAC 
AATGCTCATG GAGATGCAGA TCAACCTGCC CATGACCGCC AGCCCAGGGT CCTGCCCTCA 
GAACAAGACA GCACCAAAAA GCCCTATCGG ATAGACAGTG ACGAGGAAGA GGACTTTGAA 
AATGTAGGCA AGGTGGGGAG CCCATTGGAC TATAGCTTAG TGGACCTACC TTCCACCAAT 
GGACAGAGTC CTGGCAAAGC CATTGAAAAC TTGATTGGCA AGCCAGCTGA GAAGCCTCAG 
ACCCCCAAGG ACAGCAGCAC AGCCAGTGCA AGCCTGGCCC CCAATGGGAC AAGTGGTGGG 
CAGGAGGCAG GGGCCCCAGA AGAGGACGAA GATGAGCTTT TAAGAGTGAC TGACCTTGTT 
GATTATGTCT GTAACAGTGA ACAGTTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002699109.1
CDS	283..4572
Translation	MAAAAAAAAAMAPPGCPGSCPNFAVVCSFLERYGPLLDLPELPFPELERVLQAPPPDVGNGEVPKELVELHLKLMRKIGKSVTADRWEKYLIKICQEFNSTWAWEMEKKGYLEMSVECKLALLKYLCECQFDDNLKFKNIINEEDADTMRLQPIGRDKDGLMYWYQLDQDHNVRMYIEEQDDQDGSSWKCIVRNRNELAETLALLKAQIDPVLLKNSSQQDSSSRESPSLEEEETKKEEETTKQEEQKGSAKMKSEERPIDSEKCSTPSALEETTVKIEKEDEKELVKLPVIVKLEKPFPESEEKKIIKEESDSFKENVKPVKVEMKECKADPRDIKGSMEKLEPERLEFVGNVKSSQEITEKSTEETEKLKNDQQAKIPLKKREIKLSDDFDSPIKGPLCKSVTPTKEFLKDEIKQEEETCKRISTITALGHEGKQLVNGEVSDEKVTPHFKTEQMETKFYDSKEDSCSPSKDRSVIMEGNGAESVNSVIPSVKIGDLEKEVVPLGKDTDNSISVLETPSQKEYIDETGPSEMETSLETAEVAKDLCLKTALSATESYSMRVEEKSPKSKKDKRPPVLECLEKSKKTFLDKDIQRLSPIPEEVPKTTVESEKAGSPEAAETYPSSNVTVHCEKLASEVECQNTSSLEGHSLEKVDPEILKVDSESTKVEVDNLDNAQTSGTGDPSETKGSMQKSKLKYKLIPEEENTASENTEITSERQKEGIKLTIRISSRKKKSDSPPKILEPETKQEKTEKEEEKTNVGRTLRRSPRISRPTAKVAEIRDQKADKKRGEEDEVEEESAALQKTDKKENLKKAEKDTNSKVAKVKPKGKVRWTGSRTRGRWKYSSNDESEGSDSEKSSAASEEEEEKESEEAILADDDEPCKKCGLPNHPELILLCDSCDSGYHTACLRPPLMIIPDGEWFCPPCQHKLLCEKLEEQLQDLDVALKKKERAERRKERLVYVGISIENIIPPQEPDFSEDHEEKKKDSKKSKANLLERRSTRTRKCISYRFDEFDEAIDEAIEDDIKEADGGGVGRGKDISTITGHRGKDISTILDEERKENKRPQRAAAARRKKRRRLNDLDSDSNLDEEESEDEFKISDGSQDEFVVSDENPDESEEDPPSNDDSDTDFCSRRLRRHPSRPMRQSRRLRRKTPKRKCSDDDEEEESEENSRDSESDFSDDFSDDFVETRRRRSRRNQKRQINYKEDSESDGSQKSLRRGKEIRRVHKRRLSSSDSEESYMSKNSEDDELAKESKRSVRKRGRSTDEYSEADEEEEEGKPSRKRLHRIETDEEESCDNAHGDADQPAHDRQPRVLPSEQDSTKKPYRIDSDEEEDFENVGKVGSPLDYSLVDLPSTNGQSPGKAIENLIGKPAEKPQTPKDSSTASASLAPNGTSGGQEAGAPEEDEDELLRVTDLVDYVCNSEQL

Target ORF information:

RefSeq Version	XM_002699063.4
Organism	Bos taurus(cattle)
Definition	Bos taurus remodeling and spacing factor 1 (RSF1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002699063.4

ORF Insert Sequence:

ATGGCGGCGG CGGCGGCAGC GGCGGCGGCG ATGGCTCCTC CGGGCTGCCC GGGTTCGTGT 
CCCAACTTCG CCGTAGTCTG CTCTTTCTTG GAGCGCTACG GGCCGCTGCT CGACCTGCCT 
GAGTTGCCGT TCCCGGAGCT CGAGCGGGTG CTGCAAGCTC CGCCGCCGGA CGTCGGCAAC 
GGAGAAGTAC CTAAAGAATT GGTGGAGCTC CATTTGAAGT TGATGAGGAA GATTGGCAAA 
TCTGTTACAG CAGACAGATG GGAAAAATAC TTGATCAAGA TATGCCAAGA GTTTAATAGC 
ACCTGGGCGT GGGAGATGGA GAAGAAAGGC TATCTTGAAA TGAGTGTTGA ATGCAAGTTA 
GCACTCTTAA AGTACCTCTG TGAGTGTCAA TTTGATGACA ATCTCAAATT CAAGAATATT 
ATCAATGAGG AGGATGCCGA TACCATGCGT CTCCAGCCAA TTGGCCGTGA CAAAGATGGC 
CTCATGTACT GGTACCAGTT GGATCAAGAT CACAATGTCA GAATGTACAT AGAAGAACAA 
GATGATCAAG ATGGCTCTTC ATGGAAGTGC ATTGTGAGAA ATCGAAACGA GTTGGCTGAG 
ACACTTGCAC TCCTGAAAGC ACAAATTGAT CCTGTACTAT TGAAGAACTC TAGCCAACAG 
GACAGCTCTT CCCGGGAAAG TCCCAGCTTA GAAGAAGAGG AGACTAAAAA GGAGGAAGAA 
ACAACTAAAC AAGAGGAACA GAAAGGAAGT GCAAAGATGA AAAGTGAAGA GCGGCCAATA 
GATTCAGAAA AATGTTCTAC ACCCAGTGCT CTAGAAGAGA CTACTGTGAA AATAGAAAAA 
GAAGATGAAA AGGAACTTGT AAAACTGCCA GTCATAGTGA AGCTAGAAAA ACCTTTTCCA 
GAAAGTGAAG AAAAAAAGAT TATCAAAGAA GAAAGTGATT CCTTCAAGGA AAATGTCAAA 
CCTGTAAAAG TGGAAATGAA AGAATGTAAA GCAGATCCTA GAGATATCAA AGGTAGTATG 
GAGAAGTTAG AGCCTGAGAG GCTAGAGTTT GTTGGCAATG TTAAATCTTC TCAAGAAATT 
ACTGAAAAGT CTACTGAAGA AACCGAGAAA CTTAAAAATG ACCAGCAGGC CAAGATTCCA 
CTAAAAAAAC GAGAAATTAA ACTGAGTGAT GATTTTGATA GTCCAATCAA AGGACCTTTG 
TGTAAATCAG TTACTCCAAC AAAAGAGTTT TTGAAAGATG AAATAAAACA AGAGGAAGAG 
ACTTGTAAAA GGATCTCTAC AATCACTGCC TTGGGTCATG AAGGGAAACA GCTGGTAAAT 
GGAGAAGTTA GTGATGAAAA GGTAACTCCA CATTTTAAGA CAGAACAGAT GGAGACAAAG 
TTTTATGATT CAAAGGAAGA TAGTTGCAGC CCCTCTAAGG ACAGAAGTGT CATCATGGAG 
GGAAATGGAG CAGAGTCTGT AAATTCTGTC ATTCCAAGTG TAAAAATAGG TGATCTTGAG 
AAAGAAGTGG TCCCTTTAGG GAAAGATACA GATAATTCAA TATCAGTCTT AGAGACCCCG 
AGTCAGAAAG AGTACATAGA CGAAACTGGT CCTTCAGAAA TGGAAACCTC TCTTGAGACT 
GCTGAAGTAG CAAAGGATCT CTGTTTAAAA ACTGCTTTAT CTGCCACTGA ATCCTATAGT 
ATGAGAGTTG AAGAGAAGTC TCCCAAAAGT AAGAAGGATA AGCGCCCACC AGTCCTAGAG 
TGTCTTGAAA AGTCCAAAAA GACTTTTCTT GATAAGGACA TACAAAGATT GAGTCCAATA 
CCGGAGGAGG TTCCAAAGAC AACTGTAGAA TCAGAGAAGG CTGGGTCTCC TGAGGCAGCT 
GAAACTTATC CGTCGTCTAA CGTGACTGTC CACTGTGAGA AACTAGCCTC AGAAGTTGAG 
TGTCAGAATA CAAGTTCACT AGAAGGTCAT TCCCTGGAGA AAGTAGACCC AGAAATTTTA 
AAAGTGGATT CTGAATCAAC AAAGGTAGAA GTGGATAATC TGGACAATGC CCAGACCTCT 
GGCACAGGTG ATCCTTCTGA GACAAAGGGT TCTATGCAAA AAAGCAAATT GAAATATAAG 
TTGATTCCTG AAGAAGAAAA CACTGCCTCA GAAAACACAG AGATAACCTC CGAAAGGCAG 
AAAGAAGGCA TCAAATTAAC AATTAGGATA TCTAGTCGGA AAAAGAAGTC AGATTCTCCT 
CCCAAAATTT TAGAACCAGA AACCAAGCAA GAGAAGACAG AAAAGGAAGA AGAGAAAACA 
AACGTTGGTC GTACTTTGAG GAGGTCTCCA AGAATATCTA GACCCACAGC AAAAGTGGCT 
GAAATCAGAG ATCAGAAAGC TGATAAAAAA AGAGGGGAAG AAGATGAAGT AGAAGAAGAG 
TCAGCAGCTT TGCAAAAGAC AGACAAAAAA GAAAATTTGA AAAAAGCAGA GAAGGATACA 
AATTCTAAAG TAGCCAAGGT AAAGCCCAAA GGCAAAGTTC GATGGACTGG CTCTCGAACA 
CGTGGCAGGT GGAAATATTC CAGCAATGAT GAAAGTGAAG GGTCTGACAG TGAAAAATCA 
TCTGCAGCTT CAGAAGAGGA GGAAGAAAAG GAAAGTGAAG AAGCCATCCT AGCAGATGAT 
GATGAACCAT GCAAAAAATG TGGCCTTCCA AACCATCCTG AATTAATCCT TCTGTGTGAC 
TCTTGTGACA GTGGATACCA CACTGCCTGC CTTCGCCCTC CTCTAATGAT TATTCCAGAT 
GGAGAATGGT TCTGCCCCCC TTGCCAGCAT AAACTACTGT GTGAAAAATT AGAAGAACAA 
TTGCAAGATT TGGATGTTGC CTTAAAGAAG AAAGAGCGTG CTGAACGCAG GAAAGAACGC 
TTGGTGTATG TGGGTATTAG TATTGAAAAC ATCATTCCTC CACAAGAGCC AGATTTTTCG 
GAAGATCATG AAGAAAAGAA AAAAGATTCA AAAAAATCCA AAGCAAACTT GCTTGAAAGG 
AGGTCGACAA GAACACGGAA ATGTATAAGT TACAGATTTG ATGAGTTTGA CGAAGCAATT 
GATGAAGCTA TAGAAGATGA TATCAAAGAG GCTGATGGAG GAGGAGTTGG CCGAGGAAAA 
GATATCTCCA CCATCACAGG CCACCGTGGA AAAGACATCT CTACTATTTT GGATGAAGAA 
AGAAAAGAAA ATAAACGACC CCAGAGGGCA GCTGCTGCTC GCCGGAAGAA ACGCCGGCGA 
CTGAATGATC TGGACAGTGA CAGCAACCTG GATGAAGAGG AGAGCGAGGA TGAATTCAAG 
ATCAGTGATG GATCTCAGGA TGAATTTGTT GTATCTGATG AGAACCCAGA TGAGAGTGAA 
GAAGACCCAC CATCTAATGA TGACAGCGAC ACTGATTTCT GTAGCCGAAG ACTAAGGCGA 
CACCCCTCCC GGCCTATGAG GCAAAGCAGG CGTTTGCGGA GAAAGACCCC AAAGAGAAAG 
TGCTCTGACG ATGATGAGGA GGAAGAGTCT GAGGAGAACA GCAGAGACTC TGAAAGTGAC 
TTTAGTGATG ACTTTAGCGA TGATTTTGTA GAAACTCGGC GAAGAAGGTC TAGAAGGAAC 
CAGAAGAGAC AAATTAACTA CAAGGAAGAT TCAGAAAGTG ATGGCTCCCA GAAGAGTTTA 
CGACGTGGTA AAGAAATCAG ACGAGTTCAC AAGCGTAGGC TCTCTAGCTC AGATAGTGAA 
GAGAGCTATA TGTCCAAGAA CTCTGAAGAT GATGAGCTAG CTAAAGAATC AAAGCGGTCA 
GTTCGAAAAC GGGGTCGAAG CACAGATGAG TATTCCGAAG CAGATGAGGA GGAAGAAGAA 
GGTAAACCAT CCCGAAAACG GCTACACCGG ATCGAGACAG ATGAAGAGGA GAGTTGTGAC 
AATGCTCATG GAGATGCAGA TCAACCTGCC CATGACCGCC AGCCCAGGGT CCTGCCCTCA 
GAACAAGACA GCACCAAAAA GCCCTATCGG ATAGACAGTG ACGAGGAAGA GGACTTTGAA 
AATGTAGGCA AGGTGGGGAG CCCATTGGAC TATAGCTTAG TGGACCTACC TTCCACCAAT 
GGACAGAGTC CTGGCAAAGC CATTGAAAAC TTGATTGGCA AGCCAGCTGA GAAGCCTCAG 
ACCCCCAAGG ACAGCAGCAC AGCCAGTGCA AGCCTGGCCC CCAATGGGAC AAGTGGTGGG 
CAGGAGGCAG GGGCCCCAGA AGAGGACGAA GATGAGCTTT TAAGAGTGAC TGACCTTGTT 
GATTATGTCT GTAACAGTGA ACAGTTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RSF1 ( XM_864539.7 ) cDNA ORF clone, Bos taurus(cattle) -> XP_869632.1 Bos taurus remodeling and spacing factor 1 (RSF1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa12598
Clone ID Related Accession (Same CDS sequence)	XM_002699063.5 , XM_002699063.4 , XM_864539.7
Accession Version	XM_864539.7 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4290bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	remodeling and spacing factor 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646776.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_864539.6. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG CGGCGGCAGC GGCGGCGGCG ATGGCTCCTC CGGGCTGCCC GGGTTCGTGT 
CCCAACTTCG CCGTAGTCTG CTCTTTCTTG GAGCGCTACG GGCCGCTGCT CGACCTGCCT 
GAGTTGCCGT TCCCGGAGCT CGAGCGGGTG CTGCAAGCTC CGCCGCCGGA CGTCGGCAAC 
GGAGAAGTAC CTAAAGAATT GGTGGAGCTC CATTTGAAGT TGATGAGGAA GATTGGCAAA 
TCTGTTACAG CAGACAGATG GGAAAAATAC TTGATCAAGA TATGCCAAGA GTTTAATAGC 
ACCTGGGCGT GGGAGATGGA GAAGAAAGGC TATCTTGAAA TGAGTGTTGA ATGCAAGTTA 
GCACTCTTAA AGTACCTCTG TGAGTGTCAA TTTGATGACA ATCTCAAATT CAAGAATATT 
ATCAATGAGG AGGATGCCGA TACCATGCGT CTCCAGCCAA TTGGCCGTGA CAAAGATGGC 
CTCATGTACT GGTACCAGTT GGATCAAGAT CACAATGTCA GAATGTACAT AGAAGAACAA 
GATGATCAAG ATGGCTCTTC ATGGAAGTGC ATTGTGAGAA ATCGAAACGA GTTGGCTGAG 
ACACTTGCAC TCCTGAAAGC ACAAATTGAT CCTGTACTAT TGAAGAACTC TAGCCAACAG 
GACAGCTCTT CCCGGGAAAG TCCCAGCTTA GAAGAAGAGG AGACTAAAAA GGAGGAAGAA 
ACAACTAAAC AAGAGGAACA GAAAGGAAGT GCAAAGATGA AAAGTGAAGA GCGGCCAATA 
GATTCAGAAA AATGTTCTAC ACCCAGTGCT CTAGAAGAGA CTACTGTGAA AATAGAAAAA 
GAAGATGAAA AGGAACTTGT AAAACTGCCA GTCATAGTGA AGCTAGAAAA ACCTTTTCCA 
GAAAGTGAAG AAAAAAAGAT TATCAAAGAA GAAAGTGATT CCTTCAAGGA AAATGTCAAA 
CCTGTAAAAG TGGAAATGAA AGAATGTAAA GCAGATCCTA GAGATATCAA AGGTAGTATG 
GAGAAGTTAG AGCCTGAGAG GCTAGAGTTT GTTGGCAATG TTAAATCTTC TCAAGAAATT 
ACTGAAAAGT CTACTGAAGA AACCGAGAAA CTTAAAAATG ACCAGCAGGC CAAGATTCCA 
CTAAAAAAAC GAGAAATTAA ACTGAGTGAT GATTTTGATA GTCCAATCAA AGGACCTTTG 
TGTAAATCAG TTACTCCAAC AAAAGAGTTT TTGAAAGATG AAATAAAACA AGAGGAAGAG 
ACTTGTAAAA GGATCTCTAC AATCACTGCC TTGGGTCATG AAGGGAAACA GCTGGTAAAT 
GGAGAAGTTA GTGATGAAAA GGTAACTCCA CATTTTAAGA CAGAACAGAT GGAGACAAAG 
TTTTATGATT CAAAGGAAGA TAGTTGCAGC CCCTCTAAGG ACAGAAGTGT CATCATGGAG 
GGAAATGGAG CAGAGTCTGT AAATTCTGTC ATTCCAAGTG TAAAAATAGG TGATCTTGAG 
AAAGAAGTGG TCCCTTTAGG GAAAGATACA GATAATTCAA TATCAGTCTT AGAGACCCCG 
AGTCAGAAAG AGTACATAGA CGAAACTGGT CCTTCAGAAA TGGAAACCTC TCTTGAGACT 
GCTGAAGTAG CAAAGGATCT CTGTTTAAAA ACTGCTTTAT CTGCCACTGA ATCCTATAGT 
ATGAGAGTTG AAGAGAAGTC TCCCAAAAGT AAGAAGGATA AGCGCCCACC AGTCCTAGAG 
TGTCTTGAAA AGTCCAAAAA GACTTTTCTT GATAAGGACA TACAAAGATT GAGTCCAATA 
CCGGAGGAGG TTCCAAAGAC AACTGTAGAA TCAGAGAAGG CTGGGTCTCC TGAGGCAGCT 
GAAACTTATC CGTCGTCTAA CGTGACTGTC CACTGTGAGA AACTAGCCTC AGAAGTTGAG 
TGTCAGAATA CAAGTTCACT AGAAGGTCAT TCCCTGGAGA AAGTAGACCC AGAAATTTTA 
AAAGTGGATT CTGAATCAAC AAAGGTAGAA GTGGATAATC TGGACAATGC CCAGACCTCT 
GGCACAGGTG ATCCTTCTGA GACAAAGGGT TCTATGCAAA AAAGCAAATT GAAATATAAG 
TTGATTCCTG AAGAAGAAAA CACTGCCTCA GAAAACACAG AGATAACCTC CGAAAGGCAG 
AAAGAAGGCA TCAAATTAAC AATTAGGATA TCTAGTCGGA AAAAGAAGTC AGATTCTCCT 
CCCAAAATTT TAGAACCAGA AACCAAGCAA GAGAAGACAG AAAAGGAAGA AGAGAAAACA 
AACGTTGGTC GTACTTTGAG GAGGTCTCCA AGAATATCTA GACCCACAGC AAAAGTGGCT 
GAAATCAGAG ATCAGAAAGC TGATAAAAAA AGAGGGGAAG AAGATGAAGT AGAAGAAGAG 
TCAGCAGCTT TGCAAAAGAC AGACAAAAAA GAAAATTTGA AAAAAGCAGA GAAGGATACA 
AATTCTAAAG TAGCCAAGGT AAAGCCCAAA GGCAAAGTTC GATGGACTGG CTCTCGAACA 
CGTGGCAGGT GGAAATATTC CAGCAATGAT GAAAGTGAAG GGTCTGACAG TGAAAAATCA 
TCTGCAGCTT CAGAAGAGGA GGAAGAAAAG GAAAGTGAAG AAGCCATCCT AGCAGATGAT 
GATGAACCAT GCAAAAAATG TGGCCTTCCA AACCATCCTG AATTAATCCT TCTGTGTGAC 
TCTTGTGACA GTGGATACCA CACTGCCTGC CTTCGCCCTC CTCTAATGAT TATTCCAGAT 
GGAGAATGGT TCTGCCCCCC TTGCCAGCAT AAACTACTGT GTGAAAAATT AGAAGAACAA 
TTGCAAGATT TGGATGTTGC CTTAAAGAAG AAAGAGCGTG CTGAACGCAG GAAAGAACGC 
TTGGTGTATG TGGGTATTAG TATTGAAAAC ATCATTCCTC CACAAGAGCC AGATTTTTCG 
GAAGATCATG AAGAAAAGAA AAAAGATTCA AAAAAATCCA AAGCAAACTT GCTTGAAAGG 
AGGTCGACAA GAACACGGAA ATGTATAAGT TACAGATTTG ATGAGTTTGA CGAAGCAATT 
GATGAAGCTA TAGAAGATGA TATCAAAGAG GCTGATGGAG GAGGAGTTGG CCGAGGAAAA 
GATATCTCCA CCATCACAGG CCACCGTGGA AAAGACATCT CTACTATTTT GGATGAAGAA 
AGAAAAGAAA ATAAACGACC CCAGAGGGCA GCTGCTGCTC GCCGGAAGAA ACGCCGGCGA 
CTGAATGATC TGGACAGTGA CAGCAACCTG GATGAAGAGG AGAGCGAGGA TGAATTCAAG 
ATCAGTGATG GATCTCAGGA TGAATTTGTT GTATCTGATG AGAACCCAGA TGAGAGTGAA 
GAAGACCCAC CATCTAATGA TGACAGCGAC ACTGATTTCT GTAGCCGAAG ACTAAGGCGA 
CACCCCTCCC GGCCTATGAG GCAAAGCAGG CGTTTGCGGA GAAAGACCCC AAAGAGAAAG 
TGCTCTGACG ATGATGAGGA GGAAGAGTCT GAGGAGAACA GCAGAGACTC TGAAAGTGAC 
TTTAGTGATG ACTTTAGCGA TGATTTTGTA GAAACTCGGC GAAGAAGGTC TAGAAGGAAC 
CAGAAGAGAC AAATTAACTA CAAGGAAGAT TCAGAAAGTG ATGGCTCCCA GAAGAGTTTA 
CGACGTGGTA AAGAAATCAG ACGAGTTCAC AAGCGTAGGC TCTCTAGCTC AGATAGTGAA 
GAGAGCTATA TGTCCAAGAA CTCTGAAGAT GATGAGCTAG CTAAAGAATC AAAGCGGTCA 
GTTCGAAAAC GGGGTCGAAG CACAGATGAG TATTCCGAAG CAGATGAGGA GGAAGAAGAA 
GGTAAACCAT CCCGAAAACG GCTACACCGG ATCGAGACAG ATGAAGAGGA GAGTTGTGAC 
AATGCTCATG GAGATGCAGA TCAACCTGCC CATGACCGCC AGCCCAGGGT CCTGCCCTCA 
GAACAAGACA GCACCAAAAA GCCCTATCGG ATAGACAGTG ACGAGGAAGA GGACTTTGAA 
AATGTAGGCA AGGTGGGGAG CCCATTGGAC TATAGCTTAG TGGACCTACC TTCCACCAAT 
GGACAGAGTC CTGGCAAAGC CATTGAAAAC TTGATTGGCA AGCCAGCTGA GAAGCCTCAG 
ACCCCCAAGG ACAGCAGCAC AGCCAGTGCA AGCCTGGCCC CCAATGGGAC AAGTGGTGGG 
CAGGAGGCAG GGGCCCCAGA AGAGGACGAA GATGAGCTTT TAAGAGTGAC TGACCTTGTT 
GATTATGTCT GTAACAGTGA ACAGTTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_869632.1
CDS	283..4572
Translation	MAAAAAAAAAMAPPGCPGSCPNFAVVCSFLERYGPLLDLPELPFPELERVLQAPPPDVGNGEVPKELVELHLKLMRKIGKSVTADRWEKYLIKICQEFNSTWAWEMEKKGYLEMSVECKLALLKYLCECQFDDNLKFKNIINEEDADTMRLQPIGRDKDGLMYWYQLDQDHNVRMYIEEQDDQDGSSWKCIVRNRNELAETLALLKAQIDPVLLKNSSQQDSSSRESPSLEEEETKKEEETTKQEEQKGSAKMKSEERPIDSEKCSTPSALEETTVKIEKEDEKELVKLPVIVKLEKPFPESEEKKIIKEESDSFKENVKPVKVEMKECKADPRDIKGSMEKLEPERLEFVGNVKSSQEITEKSTEETEKLKNDQQAKIPLKKREIKLSDDFDSPIKGPLCKSVTPTKEFLKDEIKQEEETCKRISTITALGHEGKQLVNGEVSDEKVTPHFKTEQMETKFYDSKEDSCSPSKDRSVIMEGNGAESVNSVIPSVKIGDLEKEVVPLGKDTDNSISVLETPSQKEYIDETGPSEMETSLETAEVAKDLCLKTALSATESYSMRVEEKSPKSKKDKRPPVLECLEKSKKTFLDKDIQRLSPIPEEVPKTTVESEKAGSPEAAETYPSSNVTVHCEKLASEVECQNTSSLEGHSLEKVDPEILKVDSESTKVEVDNLDNAQTSGTGDPSETKGSMQKSKLKYKLIPEEENTASENTEITSERQKEGIKLTIRISSRKKKSDSPPKILEPETKQEKTEKEEEKTNVGRTLRRSPRISRPTAKVAEIRDQKADKKRGEEDEVEEESAALQKTDKKENLKKAEKDTNSKVAKVKPKGKVRWTGSRTRGRWKYSSNDESEGSDSEKSSAASEEEEEKESEEAILADDDEPCKKCGLPNHPELILLCDSCDSGYHTACLRPPLMIIPDGEWFCPPCQHKLLCEKLEEQLQDLDVALKKKERAERRKERLVYVGISIENIIPPQEPDFSEDHEEKKKDSKKSKANLLERRSTRTRKCISYRFDEFDEAIDEAIEDDIKEADGGGVGRGKDISTITGHRGKDISTILDEERKENKRPQRAAAARRKKRRRLNDLDSDSNLDEEESEDEFKISDGSQDEFVVSDENPDESEEDPPSNDDSDTDFCSRRLRRHPSRPMRQSRRLRRKTPKRKCSDDDEEEESEENSRDSESDFSDDFSDDFVETRRRRSRRNQKRQINYKEDSESDGSQKSLRRGKEIRRVHKRRLSSSDSEESYMSKNSEDDELAKESKRSVRKRGRSTDEYSEADEEEEEGKPSRKRLHRIETDEEESCDNAHGDADQPAHDRQPRVLPSEQDSTKKPYRIDSDEEEDFENVGKVGSPLDYSLVDLPSTNGQSPGKAIENLIGKPAEKPQTPKDSSTASASLAPNGTSGGQEAGAPEEDEDELLRVTDLVDYVCNSEQL

Target ORF information:

RefSeq Version	XM_864539.7
Organism	Bos taurus(cattle)
Definition	Bos taurus remodeling and spacing factor 1 (RSF1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_864539.7

ORF Insert Sequence:

ATGGCGGCGG CGGCGGCAGC GGCGGCGGCG ATGGCTCCTC CGGGCTGCCC GGGTTCGTGT 
CCCAACTTCG CCGTAGTCTG CTCTTTCTTG GAGCGCTACG GGCCGCTGCT CGACCTGCCT 
GAGTTGCCGT TCCCGGAGCT CGAGCGGGTG CTGCAAGCTC CGCCGCCGGA CGTCGGCAAC 
GGAGAAGTAC CTAAAGAATT GGTGGAGCTC CATTTGAAGT TGATGAGGAA GATTGGCAAA 
TCTGTTACAG CAGACAGATG GGAAAAATAC TTGATCAAGA TATGCCAAGA GTTTAATAGC 
ACCTGGGCGT GGGAGATGGA GAAGAAAGGC TATCTTGAAA TGAGTGTTGA ATGCAAGTTA 
GCACTCTTAA AGTACCTCTG TGAGTGTCAA TTTGATGACA ATCTCAAATT CAAGAATATT 
ATCAATGAGG AGGATGCCGA TACCATGCGT CTCCAGCCAA TTGGCCGTGA CAAAGATGGC 
CTCATGTACT GGTACCAGTT GGATCAAGAT CACAATGTCA GAATGTACAT AGAAGAACAA 
GATGATCAAG ATGGCTCTTC ATGGAAGTGC ATTGTGAGAA ATCGAAACGA GTTGGCTGAG 
ACACTTGCAC TCCTGAAAGC ACAAATTGAT CCTGTACTAT TGAAGAACTC TAGCCAACAG 
GACAGCTCTT CCCGGGAAAG TCCCAGCTTA GAAGAAGAGG AGACTAAAAA GGAGGAAGAA 
ACAACTAAAC AAGAGGAACA GAAAGGAAGT GCAAAGATGA AAAGTGAAGA GCGGCCAATA 
GATTCAGAAA AATGTTCTAC ACCCAGTGCT CTAGAAGAGA CTACTGTGAA AATAGAAAAA 
GAAGATGAAA AGGAACTTGT AAAACTGCCA GTCATAGTGA AGCTAGAAAA ACCTTTTCCA 
GAAAGTGAAG AAAAAAAGAT TATCAAAGAA GAAAGTGATT CCTTCAAGGA AAATGTCAAA 
CCTGTAAAAG TGGAAATGAA AGAATGTAAA GCAGATCCTA GAGATATCAA AGGTAGTATG 
GAGAAGTTAG AGCCTGAGAG GCTAGAGTTT GTTGGCAATG TTAAATCTTC TCAAGAAATT 
ACTGAAAAGT CTACTGAAGA AACCGAGAAA CTTAAAAATG ACCAGCAGGC CAAGATTCCA 
CTAAAAAAAC GAGAAATTAA ACTGAGTGAT GATTTTGATA GTCCAATCAA AGGACCTTTG 
TGTAAATCAG TTACTCCAAC AAAAGAGTTT TTGAAAGATG AAATAAAACA AGAGGAAGAG 
ACTTGTAAAA GGATCTCTAC AATCACTGCC TTGGGTCATG AAGGGAAACA GCTGGTAAAT 
GGAGAAGTTA GTGATGAAAA GGTAACTCCA CATTTTAAGA CAGAACAGAT GGAGACAAAG 
TTTTATGATT CAAAGGAAGA TAGTTGCAGC CCCTCTAAGG ACAGAAGTGT CATCATGGAG 
GGAAATGGAG CAGAGTCTGT AAATTCTGTC ATTCCAAGTG TAAAAATAGG TGATCTTGAG 
AAAGAAGTGG TCCCTTTAGG GAAAGATACA GATAATTCAA TATCAGTCTT AGAGACCCCG 
AGTCAGAAAG AGTACATAGA CGAAACTGGT CCTTCAGAAA TGGAAACCTC TCTTGAGACT 
GCTGAAGTAG CAAAGGATCT CTGTTTAAAA ACTGCTTTAT CTGCCACTGA ATCCTATAGT 
ATGAGAGTTG AAGAGAAGTC TCCCAAAAGT AAGAAGGATA AGCGCCCACC AGTCCTAGAG 
TGTCTTGAAA AGTCCAAAAA GACTTTTCTT GATAAGGACA TACAAAGATT GAGTCCAATA 
CCGGAGGAGG TTCCAAAGAC AACTGTAGAA TCAGAGAAGG CTGGGTCTCC TGAGGCAGCT 
GAAACTTATC CGTCGTCTAA CGTGACTGTC CACTGTGAGA AACTAGCCTC AGAAGTTGAG 
TGTCAGAATA CAAGTTCACT AGAAGGTCAT TCCCTGGAGA AAGTAGACCC AGAAATTTTA 
AAAGTGGATT CTGAATCAAC AAAGGTAGAA GTGGATAATC TGGACAATGC CCAGACCTCT 
GGCACAGGTG ATCCTTCTGA GACAAAGGGT TCTATGCAAA AAAGCAAATT GAAATATAAG 
TTGATTCCTG AAGAAGAAAA CACTGCCTCA GAAAACACAG AGATAACCTC CGAAAGGCAG 
AAAGAAGGCA TCAAATTAAC AATTAGGATA TCTAGTCGGA AAAAGAAGTC AGATTCTCCT 
CCCAAAATTT TAGAACCAGA AACCAAGCAA GAGAAGACAG AAAAGGAAGA AGAGAAAACA 
AACGTTGGTC GTACTTTGAG GAGGTCTCCA AGAATATCTA GACCCACAGC AAAAGTGGCT 
GAAATCAGAG ATCAGAAAGC TGATAAAAAA AGAGGGGAAG AAGATGAAGT AGAAGAAGAG 
TCAGCAGCTT TGCAAAAGAC AGACAAAAAA GAAAATTTGA AAAAAGCAGA GAAGGATACA 
AATTCTAAAG TAGCCAAGGT AAAGCCCAAA GGCAAAGTTC GATGGACTGG CTCTCGAACA 
CGTGGCAGGT GGAAATATTC CAGCAATGAT GAAAGTGAAG GGTCTGACAG TGAAAAATCA 
TCTGCAGCTT CAGAAGAGGA GGAAGAAAAG GAAAGTGAAG AAGCCATCCT AGCAGATGAT 
GATGAACCAT GCAAAAAATG TGGCCTTCCA AACCATCCTG AATTAATCCT TCTGTGTGAC 
TCTTGTGACA GTGGATACCA CACTGCCTGC CTTCGCCCTC CTCTAATGAT TATTCCAGAT 
GGAGAATGGT TCTGCCCCCC TTGCCAGCAT AAACTACTGT GTGAAAAATT AGAAGAACAA 
TTGCAAGATT TGGATGTTGC CTTAAAGAAG AAAGAGCGTG CTGAACGCAG GAAAGAACGC 
TTGGTGTATG TGGGTATTAG TATTGAAAAC ATCATTCCTC CACAAGAGCC AGATTTTTCG 
GAAGATCATG AAGAAAAGAA AAAAGATTCA AAAAAATCCA AAGCAAACTT GCTTGAAAGG 
AGGTCGACAA GAACACGGAA ATGTATAAGT TACAGATTTG ATGAGTTTGA CGAAGCAATT 
GATGAAGCTA TAGAAGATGA TATCAAAGAG GCTGATGGAG GAGGAGTTGG CCGAGGAAAA 
GATATCTCCA CCATCACAGG CCACCGTGGA AAAGACATCT CTACTATTTT GGATGAAGAA 
AGAAAAGAAA ATAAACGACC CCAGAGGGCA GCTGCTGCTC GCCGGAAGAA ACGCCGGCGA 
CTGAATGATC TGGACAGTGA CAGCAACCTG GATGAAGAGG AGAGCGAGGA TGAATTCAAG 
ATCAGTGATG GATCTCAGGA TGAATTTGTT GTATCTGATG AGAACCCAGA TGAGAGTGAA 
GAAGACCCAC CATCTAATGA TGACAGCGAC ACTGATTTCT GTAGCCGAAG ACTAAGGCGA 
CACCCCTCCC GGCCTATGAG GCAAAGCAGG CGTTTGCGGA GAAAGACCCC AAAGAGAAAG 
TGCTCTGACG ATGATGAGGA GGAAGAGTCT GAGGAGAACA GCAGAGACTC TGAAAGTGAC 
TTTAGTGATG ACTTTAGCGA TGATTTTGTA GAAACTCGGC GAAGAAGGTC TAGAAGGAAC 
CAGAAGAGAC AAATTAACTA CAAGGAAGAT TCAGAAAGTG ATGGCTCCCA GAAGAGTTTA 
CGACGTGGTA AAGAAATCAG ACGAGTTCAC AAGCGTAGGC TCTCTAGCTC AGATAGTGAA 
GAGAGCTATA TGTCCAAGAA CTCTGAAGAT GATGAGCTAG CTAAAGAATC AAAGCGGTCA 
GTTCGAAAAC GGGGTCGAAG CACAGATGAG TATTCCGAAG CAGATGAGGA GGAAGAAGAA 
GGTAAACCAT CCCGAAAACG GCTACACCGG ATCGAGACAG ATGAAGAGGA GAGTTGTGAC 
AATGCTCATG GAGATGCAGA TCAACCTGCC CATGACCGCC AGCCCAGGGT CCTGCCCTCA 
GAACAAGACA GCACCAAAAA GCCCTATCGG ATAGACAGTG ACGAGGAAGA GGACTTTGAA 
AATGTAGGCA AGGTGGGGAG CCCATTGGAC TATAGCTTAG TGGACCTACC TTCCACCAAT 
GGACAGAGTC CTGGCAAAGC CATTGAAAAC TTGATTGGCA AGCCAGCTGA GAAGCCTCAG 
ACCCCCAAGG ACAGCAGCAC AGCCAGTGCA AGCCTGGCCC CCAATGGGAC AAGTGGTGGG 
CAGGAGGCAG GGGCCCCAGA AGAGGACGAA GATGAGCTTT TAAGAGTGAC TGACCTTGTT 
GATTATGTCT GTAACAGTGA ACAGTTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RSF1 ( XM_010820856.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010819158.1 Bos taurus remodeling and spacing factor 1 (RSF1), transcript variant X1, mRNA.

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CloneID	OBa55069
Clone ID Related Accession (Same CDS sequence)	XM_010820856.3 , XM_010826221.2 , XM_010820856.2
Accession Version	XM_010820856.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4422bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	remodeling and spacing factor 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037356.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010820856.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG CGGCGGCAGC GGCGGCGGCG ATGGCTCCTC CGGGCTGCCC GGGTTCGTGT 
CCCAACTTCG CCGTAGTCTG CTCTTTCTTG GAGCGCTACG GGCCGCTGCT CGACCTGCCT 
GAGTTGCCGT TCCCGGAGCT CGAGCGGGTG CTGCAAGCTC CGCCGCCGGA CGTCGGCAAC 
GGAGAAGTAC CTAAAGAATT GGTGGAGCTC CATTTGAAGT TGATGAGGAA GATTGGCAAA 
TCTGTTACAG CAGACAGATG GGAAAAATAC TTGATCAAGA TATGCCAAGA GTTTAATAGC 
ACCTGGGCGT GGGAGATGGA GAAGAAAGGC TATCTTGAAA TGAGTGTTGA ATGCAAGTTA 
GCACTCTTAA AGTACCTCTG TGAGTGTCAA TTTGATGACA ATCTCAAATT CAAGAATATT 
ATCAATGAGG AGGATGCCGA TACCATGCGT CTCCAGCCAA TTGGCCGTGA CAAAGATGGC 
CTCATGTACT GGTACCAGTT GGATCAAGAT CACAATGTCA GAATGTACAT AGAAGAACAA 
GATGATCAAG ATGGCTCTTC ATGGAAGTGC ATTGTGAGAA ATCGAAACGA GTTGGCTGAG 
ACACTTGCAC TCCTGAAAGC ACAAATTGAT CCTGTACTAT TGAAGAACTC TAGCCAACAG 
GACAGCTCTT CCCGGGAAAG TCCCAGCTTA GAAGAAGAGG AGACTAAAAA GGAGGAAGAA 
ACAACTAAAC AAGGTATTGT GTGCAGACTC TGGACAGAAA GAAGGGGAAA GGGCAGAAGG 
CATAGGTCAA CTGAACCCTG TAACAGCTGT CTTGAATCCC TTCAAGTCTG TCAAGGAACA 
TTGCTTCTGC ATTGTGATTT TGAAGAGGAA CAGAAAGGAA GTGCAAAGAT GAAAAGTGAA 
GAGCGGCCAA TAGATTCAGA AAAATGTTCT ACACCCAGTG CTCTAGAAGA GACTACTGTG 
AAAATAGAAA AAGAAGATGA AAAGGAACTT GTAAAACTGC CAGTCATAGT GAAGCTAGAA 
AAACCTTTTC CAGAAAGTGA AGAAAAAAAG ATTATCAAAG AAGAAAGTGA TTCCTTCAAG 
GAAAATGTCA AACCTGTAAA AGTGGAAATG AAAGAATGTA AAGCAGATCC TAGAGATATC 
AAAGGTAGTA TGGAGAAGTT AGAGCCTGAG AGGCTAGAGT TTGTTGGCAA TGTTAAATCT 
TCTCAAGAAA TTACTGAAAA GTCTACTGAA GAAACCGAGA AACTTAAAAA TGACCAGCAG 
GCCAAGATTC CACTAAAAAA ACGAGAAATT AAACTGAGTG ATGATTTTGA TAGTCCAATC 
AAAGGACCTT TGTGTAAATC AGTTACTCCA ACAAAAGAGT TTTTGAAAGA TGAAATAAAA 
CAAGAGGAAG AGACTTGTAA AAGGATCTCT ACAATCACTG CCTTGGGTCA TGAAGGGAAA 
CAGCTGGTAA ATGGAGAAGT TAGTGATGAA AAGGTAACTC CACATTTTAA GACAGAACAG 
ATGGAGACAA AGTTTTATGA TTCAAAGGAA GATAGTTGCA GCCCCTCTAA GGACAGAAGT 
GTCATCATGG AGGGAAATGG AGCAGAGTCT GTAAATTCTG TCATTCCAAG TGTAAAAATA 
GGTGATCTTG AGAAAGAAGT GGTCCCTTTA GGGAAAGATA CAGATAATTC AATATCAGTC 
TTAGAGACCC CGAGTCAGAA AGAGTACATA GACGAAACTG GTCCTTCAGA AATGGAAACC 
TCTCTTGAGA CTGCTGAAGT AGCAAAGGAT CTCTGTTTAA AAACTGCTTT ATCTGCCACT 
GAATCCTATA GTATGAGAGT TGAAGAGAAG TCTCCCAAAA GTAAGAAGGA TAAGCGCCCA 
CCAGTCCTAG AGTGTCTTGA AAAGTCCAAA AAGACTTTTC TTGATAAGGA CATACAAAGA 
TTGAGTCCAA TACCGGAGGA GGTTCCAAAG ACAACTGTAG AATCAGAGAA GGCTGGGTCT 
CCTGAGGCAG CTGAAACTTA TCCGTCGTCT AACGTGACTG TCCACTGTGA GAAACTAGCC 
TCAGAAGTTG AGTGTCAGAA TACAAGTTCA CTAGAAGGTC ATTCCCTGGA GAAAGTAGAC 
CCAGAAATTT TAAAAGTGGA TTCTGAATCA ACAAAGGTAG AAGTGGATAA TCTGGACAAT 
GCCCAGACCT CTGGCACAGG TGATCCTTCT GAGACAAAGG GTTCTATGCA AAAAAGCAAA 
TTGAAATATA AGTTGATTCC TGAAGAAGAA AACACTGCCT CAGAAAACAC AGAGATAACC 
TCCGAAAGGC AGAAAGAAGG CATCAAATTA ACAATTAGGA TATCTAGTCG GAAAAAGAAG 
TCAGATTCTC CTCCCAAAAT TTTAGAACCA GAAACCAAGC AAGAGAAGAC AGAAAAGGAA 
GAAGAGAAAA CAAACGTTGG TCGTACTTTG AGGAGGTCTC CAAGAATATC TAGACCCACA 
GCAAAAGTGG CTGAAATCAG AGATCAGAAA GCTGATAAAA AAAGAGGGGA AGAAGATGAA 
GTAGAAGAAG AGTCAGCAGC TTTGCAAAAG ACAGACAAAA AAGAAAATTT GAAAAAAGCA 
GAGAAGGATA CAAATTCTAA AGTAGCCAAG GTAAAGCCCA AAGGCAAAGT TCGATGGACT 
GGCTCTCGAA CACGTGGCAG GTGGAAATAT TCCAGCAATG ATGAAAGTGA AGGGTCTGAC 
AGTGAAAAAT CATCTGCAGC TTCAGAAGAG GAGGAAGAAA AGGAAAGTGA AGAAGCCATC 
CTAGCAGATG ATGATGAACC ATGCAAAAAA TGTGGCCTTC CAAACCATCC TGAATTAATC 
CTTCTGTGTG ACTCTTGTGA CAGTGGATAC CACACTGCCT GCCTTCGCCC TCCTCTAATG 
ATTATTCCAG ATGGAGAATG GTTCTGCCCC CCTTGCCAGC ATAAACTACT GTGTGAAAAA 
TTAGAAGAAC AATTGCAAGA TTTGGATGTT GCCTTAAAGA AGAAAGAGCG TGCTGAACGC 
AGGAAAGAAC GCTTGGTGTA TGTGGGTATT AGTATTGAAA ACATCATTCC TCCACAAGAG 
CCAGATTTTT CGGAAGATCA TGAAGAAAAG AAAAAAGATT CAAAAAAATC CAAAGCAAAC 
TTGCTTGAAA GGAGGTCGAC AAGAACACGG AAATGTATAA GTTACAGATT TGATGAGTTT 
GACGAAGCAA TTGATGAAGC TATAGAAGAT GATATCAAAG AGGCTGATGG AGGAGGAGTT 
GGCCGAGGAA AAGATATCTC CACCATCACA GGCCACCGTG GAAAAGACAT CTCTACTATT 
TTGGATGAAG AAAGAAAAGA AAATAAACGA CCCCAGAGGG CAGCTGCTGC TCGCCGGAAG 
AAACGCCGGC GACTGAATGA TCTGGACAGT GACAGCAACC TGGATGAAGA GGAGAGCGAG 
GATGAATTCA AGATCAGTGA TGGATCTCAG GATGAATTTG TTGTATCTGA TGAGAACCCA 
GATGAGAGTG AAGAAGACCC ACCATCTAAT GATGACAGCG ACACTGATTT CTGTAGCCGA 
AGACTAAGGC GACACCCCTC CCGGCCTATG AGGCAAAGCA GGCGTTTGCG GAGAAAGACC 
CCAAAGAGAA AGTGCTCTGA CGATGATGAG GAGGAAGAGT CTGAGGAGAA CAGCAGAGAC 
TCTGAAAGTG ACTTTAGTGA TGACTTTAGC GATGATTTTG TAGAAACTCG GCGAAGAAGG 
TCTAGAAGGA ACCAGAAGAG ACAAATTAAC TACAAGGAAG ATTCAGAAAG TGATGGCTCC 
CAGAAGAGTT TACGACGTGG TAAAGAAATC AGACGAGTTC ACAAGCGTAG GCTCTCTAGC 
TCAGATAGTG AAGAGAGCTA TATGTCCAAG AACTCTGAAG ATGATGAGCT AGCTAAAGAA 
TCAAAGCGGT CAGTTCGAAA ACGGGGTCGA AGCACAGATG AGTATTCCGA AGCAGATGAG 
GAGGAAGAAG AAGGTAAACC ATCCCGAAAA CGGCTACACC GGATCGAGAC AGATGAAGAG 
GAGAGTTGTG ACAATGCTCA TGGAGATGCA GATCAACCTG CCCATGACCG CCAGCCCAGG 
GTCCTGCCCT CAGAACAAGA CAGCACCAAA AAGCCCTATC GGATAGACAG TGACGAGGAA 
GAGGACTTTG AAAATGTAGG CAAGGTGGGG AGCCCATTGG ACTATAGCTT AGTGGACCTA 
CCTTCCACCA ATGGACAGAG TCCTGGCAAA GCCATTGAAA ACTTGATTGG CAAGCCAGCT 
GAGAAGCCTC AGACCCCCAA GGACAGCAGC ACAGCCAGTG CAAGCCTGGC CCCCAATGGG 
ACAAGTGGTG GGCAGGAGGC AGGGGCCCCA GAAGAGGACG AAGATGAGCT TTTAAGAGTG 
ACTGACCTTG TTGATTATGT CTGTAACAGT GAACAGTTAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010819158.1
CDS	280..4701
Translation	MAAAAAAAAAMAPPGCPGSCPNFAVVCSFLERYGPLLDLPELPFPELERVLQAPPPDVGNGEVPKELVELHLKLMRKIGKSVTADRWEKYLIKICQEFNSTWAWEMEKKGYLEMSVECKLALLKYLCECQFDDNLKFKNIINEEDADTMRLQPIGRDKDGLMYWYQLDQDHNVRMYIEEQDDQDGSSWKCIVRNRNELAETLALLKAQIDPVLLKNSSQQDSSSRESPSLEEEETKKEEETTKQGIVCRLWTERRGKGRRHRSTEPCNSCLESLQVCQGTLLLHCDFEEEQKGSAKMKSEERPIDSEKCSTPSALEETTVKIEKEDEKELVKLPVIVKLEKPFPESEEKKIIKEESDSFKENVKPVKVEMKECKADPRDIKGSMEKLEPERLEFVGNVKSSQEITEKSTEETEKLKNDQQAKIPLKKREIKLSDDFDSPIKGPLCKSVTPTKEFLKDEIKQEEETCKRISTITALGHEGKQLVNGEVSDEKVTPHFKTEQMETKFYDSKEDSCSPSKDRSVIMEGNGAESVNSVIPSVKIGDLEKEVVPLGKDTDNSISVLETPSQKEYIDETGPSEMETSLETAEVAKDLCLKTALSATESYSMRVEEKSPKSKKDKRPPVLECLEKSKKTFLDKDIQRLSPIPEEVPKTTVESEKAGSPEAAETYPSSNVTVHCEKLASEVECQNTSSLEGHSLEKVDPEILKVDSESTKVEVDNLDNAQTSGTGDPSETKGSMQKSKLKYKLIPEEENTASENTEITSERQKEGIKLTIRISSRKKKSDSPPKILEPETKQEKTEKEEEKTNVGRTLRRSPRISRPTAKVAEIRDQKADKKRGEEDEVEEESAALQKTDKKENLKKAEKDTNSKVAKVKPKGKVRWTGSRTRGRWKYSSNDESEGSDSEKSSAASEEEEEKESEEAILADDDEPCKKCGLPNHPELILLCDSCDSGYHTACLRPPLMIIPDGEWFCPPCQHKLLCEKLEEQLQDLDVALKKKERAERRKERLVYVGISIENIIPPQEPDFSEDHEEKKKDSKKSKANLLERRSTRTRKCISYRFDEFDEAIDEAIEDDIKEADGGGVGRGKDISTITGHRGKDISTILDEERKENKRPQRAAAARRKKRRRLNDLDSDSNLDEEESEDEFKISDGSQDEFVVSDENPDESEEDPPSNDDSDTDFCSRRLRRHPSRPMRQSRRLRRKTPKRKCSDDDEEEESEENSRDSESDFSDDFSDDFVETRRRRSRRNQKRQINYKEDSESDGSQKSLRRGKEIRRVHKRRLSSSDSEESYMSKNSEDDELAKESKRSVRKRGRSTDEYSEADEEEEEGKPSRKRLHRIETDEEESCDNAHGDADQPAHDRQPRVLPSEQDSTKKPYRIDSDEEEDFENVGKVGSPLDYSLVDLPSTNGQSPGKAIENLIGKPAEKPQTPKDSSTASASLAPNGTSGGQEAGAPEEDEDELLRVTDLVDYVCNSEQL

Target ORF information:

RefSeq Version	XM_010820856.3
Organism	Bos taurus(cattle)
Definition	Bos taurus remodeling and spacing factor 1 (RSF1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010820856.3

ORF Insert Sequence:

ATGGCGGCGG CGGCGGCAGC GGCGGCGGCG ATGGCTCCTC CGGGCTGCCC GGGTTCGTGT 
CCCAACTTCG CCGTAGTCTG CTCTTTCTTG GAGCGCTACG GGCCGCTGCT CGACCTGCCT 
GAGTTGCCGT TCCCGGAGCT CGAGCGGGTG CTGCAAGCTC CGCCGCCGGA CGTCGGCAAC 
GGAGAAGTAC CTAAAGAATT GGTGGAGCTC CATTTGAAGT TGATGAGGAA GATTGGCAAA 
TCTGTTACAG CAGACAGATG GGAAAAATAC TTGATCAAGA TATGCCAAGA GTTTAATAGC 
ACCTGGGCGT GGGAGATGGA GAAGAAAGGC TATCTTGAAA TGAGTGTTGA ATGCAAGTTA 
GCACTCTTAA AGTACCTCTG TGAGTGTCAA TTTGATGACA ATCTCAAATT CAAGAATATT 
ATCAATGAGG AGGATGCCGA TACCATGCGT CTCCAGCCAA TTGGCCGTGA CAAAGATGGC 
CTCATGTACT GGTACCAGTT GGATCAAGAT CACAATGTCA GAATGTACAT AGAAGAACAA 
GATGATCAAG ATGGCTCTTC ATGGAAGTGC ATTGTGAGAA ATCGAAACGA GTTGGCTGAG 
ACACTTGCAC TCCTGAAAGC ACAAATTGAT CCTGTACTAT TGAAGAACTC TAGCCAACAG 
GACAGCTCTT CCCGGGAAAG TCCCAGCTTA GAAGAAGAGG AGACTAAAAA GGAGGAAGAA 
ACAACTAAAC AAGGTATTGT GTGCAGACTC TGGACAGAAA GAAGGGGAAA GGGCAGAAGG 
CATAGGTCAA CTGAACCCTG TAACAGCTGT CTTGAATCCC TTCAAGTCTG TCAAGGAACA 
TTGCTTCTGC ATTGTGATTT TGAAGAGGAA CAGAAAGGAA GTGCAAAGAT GAAAAGTGAA 
GAGCGGCCAA TAGATTCAGA AAAATGTTCT ACACCCAGTG CTCTAGAAGA GACTACTGTG 
AAAATAGAAA AAGAAGATGA AAAGGAACTT GTAAAACTGC CAGTCATAGT GAAGCTAGAA 
AAACCTTTTC CAGAAAGTGA AGAAAAAAAG ATTATCAAAG AAGAAAGTGA TTCCTTCAAG 
GAAAATGTCA AACCTGTAAA AGTGGAAATG AAAGAATGTA AAGCAGATCC TAGAGATATC 
AAAGGTAGTA TGGAGAAGTT AGAGCCTGAG AGGCTAGAGT TTGTTGGCAA TGTTAAATCT 
TCTCAAGAAA TTACTGAAAA GTCTACTGAA GAAACCGAGA AACTTAAAAA TGACCAGCAG 
GCCAAGATTC CACTAAAAAA ACGAGAAATT AAACTGAGTG ATGATTTTGA TAGTCCAATC 
AAAGGACCTT TGTGTAAATC AGTTACTCCA ACAAAAGAGT TTTTGAAAGA TGAAATAAAA 
CAAGAGGAAG AGACTTGTAA AAGGATCTCT ACAATCACTG CCTTGGGTCA TGAAGGGAAA 
CAGCTGGTAA ATGGAGAAGT TAGTGATGAA AAGGTAACTC CACATTTTAA GACAGAACAG 
ATGGAGACAA AGTTTTATGA TTCAAAGGAA GATAGTTGCA GCCCCTCTAA GGACAGAAGT 
GTCATCATGG AGGGAAATGG AGCAGAGTCT GTAAATTCTG TCATTCCAAG TGTAAAAATA 
GGTGATCTTG AGAAAGAAGT GGTCCCTTTA GGGAAAGATA CAGATAATTC AATATCAGTC 
TTAGAGACCC CGAGTCAGAA AGAGTACATA GACGAAACTG GTCCTTCAGA AATGGAAACC 
TCTCTTGAGA CTGCTGAAGT AGCAAAGGAT CTCTGTTTAA AAACTGCTTT ATCTGCCACT 
GAATCCTATA GTATGAGAGT TGAAGAGAAG TCTCCCAAAA GTAAGAAGGA TAAGCGCCCA 
CCAGTCCTAG AGTGTCTTGA AAAGTCCAAA AAGACTTTTC TTGATAAGGA CATACAAAGA 
TTGAGTCCAA TACCGGAGGA GGTTCCAAAG ACAACTGTAG AATCAGAGAA GGCTGGGTCT 
CCTGAGGCAG CTGAAACTTA TCCGTCGTCT AACGTGACTG TCCACTGTGA GAAACTAGCC 
TCAGAAGTTG AGTGTCAGAA TACAAGTTCA CTAGAAGGTC ATTCCCTGGA GAAAGTAGAC 
CCAGAAATTT TAAAAGTGGA TTCTGAATCA ACAAAGGTAG AAGTGGATAA TCTGGACAAT 
GCCCAGACCT CTGGCACAGG TGATCCTTCT GAGACAAAGG GTTCTATGCA AAAAAGCAAA 
TTGAAATATA AGTTGATTCC TGAAGAAGAA AACACTGCCT CAGAAAACAC AGAGATAACC 
TCCGAAAGGC AGAAAGAAGG CATCAAATTA ACAATTAGGA TATCTAGTCG GAAAAAGAAG 
TCAGATTCTC CTCCCAAAAT TTTAGAACCA GAAACCAAGC AAGAGAAGAC AGAAAAGGAA 
GAAGAGAAAA CAAACGTTGG TCGTACTTTG AGGAGGTCTC CAAGAATATC TAGACCCACA 
GCAAAAGTGG CTGAAATCAG AGATCAGAAA GCTGATAAAA AAAGAGGGGA AGAAGATGAA 
GTAGAAGAAG AGTCAGCAGC TTTGCAAAAG ACAGACAAAA AAGAAAATTT GAAAAAAGCA 
GAGAAGGATA CAAATTCTAA AGTAGCCAAG GTAAAGCCCA AAGGCAAAGT TCGATGGACT 
GGCTCTCGAA CACGTGGCAG GTGGAAATAT TCCAGCAATG ATGAAAGTGA AGGGTCTGAC 
AGTGAAAAAT CATCTGCAGC TTCAGAAGAG GAGGAAGAAA AGGAAAGTGA AGAAGCCATC 
CTAGCAGATG ATGATGAACC ATGCAAAAAA TGTGGCCTTC CAAACCATCC TGAATTAATC 
CTTCTGTGTG ACTCTTGTGA CAGTGGATAC CACACTGCCT GCCTTCGCCC TCCTCTAATG 
ATTATTCCAG ATGGAGAATG GTTCTGCCCC CCTTGCCAGC ATAAACTACT GTGTGAAAAA 
TTAGAAGAAC AATTGCAAGA TTTGGATGTT GCCTTAAAGA AGAAAGAGCG TGCTGAACGC 
AGGAAAGAAC GCTTGGTGTA TGTGGGTATT AGTATTGAAA ACATCATTCC TCCACAAGAG 
CCAGATTTTT CGGAAGATCA TGAAGAAAAG AAAAAAGATT CAAAAAAATC CAAAGCAAAC 
TTGCTTGAAA GGAGGTCGAC AAGAACACGG AAATGTATAA GTTACAGATT TGATGAGTTT 
GACGAAGCAA TTGATGAAGC TATAGAAGAT GATATCAAAG AGGCTGATGG AGGAGGAGTT 
GGCCGAGGAA AAGATATCTC CACCATCACA GGCCACCGTG GAAAAGACAT CTCTACTATT 
TTGGATGAAG AAAGAAAAGA AAATAAACGA CCCCAGAGGG CAGCTGCTGC TCGCCGGAAG 
AAACGCCGGC GACTGAATGA TCTGGACAGT GACAGCAACC TGGATGAAGA GGAGAGCGAG 
GATGAATTCA AGATCAGTGA TGGATCTCAG GATGAATTTG TTGTATCTGA TGAGAACCCA 
GATGAGAGTG AAGAAGACCC ACCATCTAAT GATGACAGCG ACACTGATTT CTGTAGCCGA 
AGACTAAGGC GACACCCCTC CCGGCCTATG AGGCAAAGCA GGCGTTTGCG GAGAAAGACC 
CCAAAGAGAA AGTGCTCTGA CGATGATGAG GAGGAAGAGT CTGAGGAGAA CAGCAGAGAC 
TCTGAAAGTG ACTTTAGTGA TGACTTTAGC GATGATTTTG TAGAAACTCG GCGAAGAAGG 
TCTAGAAGGA ACCAGAAGAG ACAAATTAAC TACAAGGAAG ATTCAGAAAG TGATGGCTCC 
CAGAAGAGTT TACGACGTGG TAAAGAAATC AGACGAGTTC ACAAGCGTAG GCTCTCTAGC 
TCAGATAGTG AAGAGAGCTA TATGTCCAAG AACTCTGAAG ATGATGAGCT AGCTAAAGAA 
TCAAAGCGGT CAGTTCGAAA ACGGGGTCGA AGCACAGATG AGTATTCCGA AGCAGATGAG 
GAGGAAGAAG AAGGTAAACC ATCCCGAAAA CGGCTACACC GGATCGAGAC AGATGAAGAG 
GAGAGTTGTG ACAATGCTCA TGGAGATGCA GATCAACCTG CCCATGACCG CCAGCCCAGG 
GTCCTGCCCT CAGAACAAGA CAGCACCAAA AAGCCCTATC GGATAGACAG TGACGAGGAA 
GAGGACTTTG AAAATGTAGG CAAGGTGGGG AGCCCATTGG ACTATAGCTT AGTGGACCTA 
CCTTCCACCA ATGGACAGAG TCCTGGCAAA GCCATTGAAA ACTTGATTGG CAAGCCAGCT 
GAGAAGCCTC AGACCCCCAA GGACAGCAGC ACAGCCAGTG CAAGCCTGGC CCCCAATGGG 
ACAAGTGGTG GGCAGGAGGC AGGGGCCCCA GAAGAGGACG AAGATGAGCT TTTAAGAGTG 
ACTGACCTTG TTGATTATGT CTGTAACAGT GAACAGTTAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RSF1 ( XM_010826221.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010824523.1 Bos taurus remodeling and spacing factor 1 (RSF1), transcript variant X1, mRNA.

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CloneID	OBa55069
Clone ID Related Accession (Same CDS sequence)	XM_010820856.3 , XM_010826221.2 , XM_010820856.2
Accession Version	XM_010826221.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4422bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	remodeling and spacing factor 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646776.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010826221.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG CGGCGGCAGC GGCGGCGGCG ATGGCTCCTC CGGGCTGCCC GGGTTCGTGT 
CCCAACTTCG CCGTAGTCTG CTCTTTCTTG GAGCGCTACG GGCCGCTGCT CGACCTGCCT 
GAGTTGCCGT TCCCGGAGCT CGAGCGGGTG CTGCAAGCTC CGCCGCCGGA CGTCGGCAAC 
GGAGAAGTAC CTAAAGAATT GGTGGAGCTC CATTTGAAGT TGATGAGGAA GATTGGCAAA 
TCTGTTACAG CAGACAGATG GGAAAAATAC TTGATCAAGA TATGCCAAGA GTTTAATAGC 
ACCTGGGCGT GGGAGATGGA GAAGAAAGGC TATCTTGAAA TGAGTGTTGA ATGCAAGTTA 
GCACTCTTAA AGTACCTCTG TGAGTGTCAA TTTGATGACA ATCTCAAATT CAAGAATATT 
ATCAATGAGG AGGATGCCGA TACCATGCGT CTCCAGCCAA TTGGCCGTGA CAAAGATGGC 
CTCATGTACT GGTACCAGTT GGATCAAGAT CACAATGTCA GAATGTACAT AGAAGAACAA 
GATGATCAAG ATGGCTCTTC ATGGAAGTGC ATTGTGAGAA ATCGAAACGA GTTGGCTGAG 
ACACTTGCAC TCCTGAAAGC ACAAATTGAT CCTGTACTAT TGAAGAACTC TAGCCAACAG 
GACAGCTCTT CCCGGGAAAG TCCCAGCTTA GAAGAAGAGG AGACTAAAAA GGAGGAAGAA 
ACAACTAAAC AAGGTATTGT GTGCAGACTC TGGACAGAAA GAAGGGGAAA GGGCAGAAGG 
CATAGGTCAA CTGAACCCTG TAACAGCTGT CTTGAATCCC TTCAAGTCTG TCAAGGAACA 
TTGCTTCTGC ATTGTGATTT TGAAGAGGAA CAGAAAGGAA GTGCAAAGAT GAAAAGTGAA 
GAGCGGCCAA TAGATTCAGA AAAATGTTCT ACACCCAGTG CTCTAGAAGA GACTACTGTG 
AAAATAGAAA AAGAAGATGA AAAGGAACTT GTAAAACTGC CAGTCATAGT GAAGCTAGAA 
AAACCTTTTC CAGAAAGTGA AGAAAAAAAG ATTATCAAAG AAGAAAGTGA TTCCTTCAAG 
GAAAATGTCA AACCTGTAAA AGTGGAAATG AAAGAATGTA AAGCAGATCC TAGAGATATC 
AAAGGTAGTA TGGAGAAGTT AGAGCCTGAG AGGCTAGAGT TTGTTGGCAA TGTTAAATCT 
TCTCAAGAAA TTACTGAAAA GTCTACTGAA GAAACCGAGA AACTTAAAAA TGACCAGCAG 
GCCAAGATTC CACTAAAAAA ACGAGAAATT AAACTGAGTG ATGATTTTGA TAGTCCAATC 
AAAGGACCTT TGTGTAAATC AGTTACTCCA ACAAAAGAGT TTTTGAAAGA TGAAATAAAA 
CAAGAGGAAG AGACTTGTAA AAGGATCTCT ACAATCACTG CCTTGGGTCA TGAAGGGAAA 
CAGCTGGTAA ATGGAGAAGT TAGTGATGAA AAGGTAACTC CACATTTTAA GACAGAACAG 
ATGGAGACAA AGTTTTATGA TTCAAAGGAA GATAGTTGCA GCCCCTCTAA GGACAGAAGT 
GTCATCATGG AGGGAAATGG AGCAGAGTCT GTAAATTCTG TCATTCCAAG TGTAAAAATA 
GGTGATCTTG AGAAAGAAGT GGTCCCTTTA GGGAAAGATA CAGATAATTC AATATCAGTC 
TTAGAGACCC CGAGTCAGAA AGAGTACATA GACGAAACTG GTCCTTCAGA AATGGAAACC 
TCTCTTGAGA CTGCTGAAGT AGCAAAGGAT CTCTGTTTAA AAACTGCTTT ATCTGCCACT 
GAATCCTATA GTATGAGAGT TGAAGAGAAG TCTCCCAAAA GTAAGAAGGA TAAGCGCCCA 
CCAGTCCTAG AGTGTCTTGA AAAGTCCAAA AAGACTTTTC TTGATAAGGA CATACAAAGA 
TTGAGTCCAA TACCGGAGGA GGTTCCAAAG ACAACTGTAG AATCAGAGAA GGCTGGGTCT 
CCTGAGGCAG CTGAAACTTA TCCGTCGTCT AACGTGACTG TCCACTGTGA GAAACTAGCC 
TCAGAAGTTG AGTGTCAGAA TACAAGTTCA CTAGAAGGTC ATTCCCTGGA GAAAGTAGAC 
CCAGAAATTT TAAAAGTGGA TTCTGAATCA ACAAAGGTAG AAGTGGATAA TCTGGACAAT 
GCCCAGACCT CTGGCACAGG TGATCCTTCT GAGACAAAGG GTTCTATGCA AAAAAGCAAA 
TTGAAATATA AGTTGATTCC TGAAGAAGAA AACACTGCCT CAGAAAACAC AGAGATAACC 
TCCGAAAGGC AGAAAGAAGG CATCAAATTA ACAATTAGGA TATCTAGTCG GAAAAAGAAG 
TCAGATTCTC CTCCCAAAAT TTTAGAACCA GAAACCAAGC AAGAGAAGAC AGAAAAGGAA 
GAAGAGAAAA CAAACGTTGG TCGTACTTTG AGGAGGTCTC CAAGAATATC TAGACCCACA 
GCAAAAGTGG CTGAAATCAG AGATCAGAAA GCTGATAAAA AAAGAGGGGA AGAAGATGAA 
GTAGAAGAAG AGTCAGCAGC TTTGCAAAAG ACAGACAAAA AAGAAAATTT GAAAAAAGCA 
GAGAAGGATA CAAATTCTAA AGTAGCCAAG GTAAAGCCCA AAGGCAAAGT TCGATGGACT 
GGCTCTCGAA CACGTGGCAG GTGGAAATAT TCCAGCAATG ATGAAAGTGA AGGGTCTGAC 
AGTGAAAAAT CATCTGCAGC TTCAGAAGAG GAGGAAGAAA AGGAAAGTGA AGAAGCCATC 
CTAGCAGATG ATGATGAACC ATGCAAAAAA TGTGGCCTTC CAAACCATCC TGAATTAATC 
CTTCTGTGTG ACTCTTGTGA CAGTGGATAC CACACTGCCT GCCTTCGCCC TCCTCTAATG 
ATTATTCCAG ATGGAGAATG GTTCTGCCCC CCTTGCCAGC ATAAACTACT GTGTGAAAAA 
TTAGAAGAAC AATTGCAAGA TTTGGATGTT GCCTTAAAGA AGAAAGAGCG TGCTGAACGC 
AGGAAAGAAC GCTTGGTGTA TGTGGGTATT AGTATTGAAA ACATCATTCC TCCACAAGAG 
CCAGATTTTT CGGAAGATCA TGAAGAAAAG AAAAAAGATT CAAAAAAATC CAAAGCAAAC 
TTGCTTGAAA GGAGGTCGAC AAGAACACGG AAATGTATAA GTTACAGATT TGATGAGTTT 
GACGAAGCAA TTGATGAAGC TATAGAAGAT GATATCAAAG AGGCTGATGG AGGAGGAGTT 
GGCCGAGGAA AAGATATCTC CACCATCACA GGCCACCGTG GAAAAGACAT CTCTACTATT 
TTGGATGAAG AAAGAAAAGA AAATAAACGA CCCCAGAGGG CAGCTGCTGC TCGCCGGAAG 
AAACGCCGGC GACTGAATGA TCTGGACAGT GACAGCAACC TGGATGAAGA GGAGAGCGAG 
GATGAATTCA AGATCAGTGA TGGATCTCAG GATGAATTTG TTGTATCTGA TGAGAACCCA 
GATGAGAGTG AAGAAGACCC ACCATCTAAT GATGACAGCG ACACTGATTT CTGTAGCCGA 
AGACTAAGGC GACACCCCTC CCGGCCTATG AGGCAAAGCA GGCGTTTGCG GAGAAAGACC 
CCAAAGAGAA AGTGCTCTGA CGATGATGAG GAGGAAGAGT CTGAGGAGAA CAGCAGAGAC 
TCTGAAAGTG ACTTTAGTGA TGACTTTAGC GATGATTTTG TAGAAACTCG GCGAAGAAGG 
TCTAGAAGGA ACCAGAAGAG ACAAATTAAC TACAAGGAAG ATTCAGAAAG TGATGGCTCC 
CAGAAGAGTT TACGACGTGG TAAAGAAATC AGACGAGTTC ACAAGCGTAG GCTCTCTAGC 
TCAGATAGTG AAGAGAGCTA TATGTCCAAG AACTCTGAAG ATGATGAGCT AGCTAAAGAA 
TCAAAGCGGT CAGTTCGAAA ACGGGGTCGA AGCACAGATG AGTATTCCGA AGCAGATGAG 
GAGGAAGAAG AAGGTAAACC ATCCCGAAAA CGGCTACACC GGATCGAGAC AGATGAAGAG 
GAGAGTTGTG ACAATGCTCA TGGAGATGCA GATCAACCTG CCCATGACCG CCAGCCCAGG 
GTCCTGCCCT CAGAACAAGA CAGCACCAAA AAGCCCTATC GGATAGACAG TGACGAGGAA 
GAGGACTTTG AAAATGTAGG CAAGGTGGGG AGCCCATTGG ACTATAGCTT AGTGGACCTA 
CCTTCCACCA ATGGACAGAG TCCTGGCAAA GCCATTGAAA ACTTGATTGG CAAGCCAGCT 
GAGAAGCCTC AGACCCCCAA GGACAGCAGC ACAGCCAGTG CAAGCCTGGC CCCCAATGGG 
ACAAGTGGTG GGCAGGAGGC AGGGGCCCCA GAAGAGGACG AAGATGAGCT TTTAAGAGTG 
ACTGACCTTG TTGATTATGT CTGTAACAGT GAACAGTTAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010824523.1
CDS	284..4705
Translation	MAAAAAAAAAMAPPGCPGSCPNFAVVCSFLERYGPLLDLPELPFPELERVLQAPPPDVGNGEVPKELVELHLKLMRKIGKSVTADRWEKYLIKICQEFNSTWAWEMEKKGYLEMSVECKLALLKYLCECQFDDNLKFKNIINEEDADTMRLQPIGRDKDGLMYWYQLDQDHNVRMYIEEQDDQDGSSWKCIVRNRNELAETLALLKAQIDPVLLKNSSQQDSSSRESPSLEEEETKKEEETTKQGIVCRLWTERRGKGRRHRSTEPCNSCLESLQVCQGTLLLHCDFEEEQKGSAKMKSEERPIDSEKCSTPSALEETTVKIEKEDEKELVKLPVIVKLEKPFPESEEKKIIKEESDSFKENVKPVKVEMKECKADPRDIKGSMEKLEPERLEFVGNVKSSQEITEKSTEETEKLKNDQQAKIPLKKREIKLSDDFDSPIKGPLCKSVTPTKEFLKDEIKQEEETCKRISTITALGHEGKQLVNGEVSDEKVTPHFKTEQMETKFYDSKEDSCSPSKDRSVIMEGNGAESVNSVIPSVKIGDLEKEVVPLGKDTDNSISVLETPSQKEYIDETGPSEMETSLETAEVAKDLCLKTALSATESYSMRVEEKSPKSKKDKRPPVLECLEKSKKTFLDKDIQRLSPIPEEVPKTTVESEKAGSPEAAETYPSSNVTVHCEKLASEVECQNTSSLEGHSLEKVDPEILKVDSESTKVEVDNLDNAQTSGTGDPSETKGSMQKSKLKYKLIPEEENTASENTEITSERQKEGIKLTIRISSRKKKSDSPPKILEPETKQEKTEKEEEKTNVGRTLRRSPRISRPTAKVAEIRDQKADKKRGEEDEVEEESAALQKTDKKENLKKAEKDTNSKVAKVKPKGKVRWTGSRTRGRWKYSSNDESEGSDSEKSSAASEEEEEKESEEAILADDDEPCKKCGLPNHPELILLCDSCDSGYHTACLRPPLMIIPDGEWFCPPCQHKLLCEKLEEQLQDLDVALKKKERAERRKERLVYVGISIENIIPPQEPDFSEDHEEKKKDSKKSKANLLERRSTRTRKCISYRFDEFDEAIDEAIEDDIKEADGGGVGRGKDISTITGHRGKDISTILDEERKENKRPQRAAAARRKKRRRLNDLDSDSNLDEEESEDEFKISDGSQDEFVVSDENPDESEEDPPSNDDSDTDFCSRRLRRHPSRPMRQSRRLRRKTPKRKCSDDDEEEESEENSRDSESDFSDDFSDDFVETRRRRSRRNQKRQINYKEDSESDGSQKSLRRGKEIRRVHKRRLSSSDSEESYMSKNSEDDELAKESKRSVRKRGRSTDEYSEADEEEEEGKPSRKRLHRIETDEEESCDNAHGDADQPAHDRQPRVLPSEQDSTKKPYRIDSDEEEDFENVGKVGSPLDYSLVDLPSTNGQSPGKAIENLIGKPAEKPQTPKDSSTASASLAPNGTSGGQEAGAPEEDEDELLRVTDLVDYVCNSEQL

Target ORF information:

RefSeq Version	XM_010826221.2
Organism	Bos taurus(cattle)
Definition	Bos taurus remodeling and spacing factor 1 (RSF1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010826221.2

ORF Insert Sequence:

ATGGCGGCGG CGGCGGCAGC GGCGGCGGCG ATGGCTCCTC CGGGCTGCCC GGGTTCGTGT 
CCCAACTTCG CCGTAGTCTG CTCTTTCTTG GAGCGCTACG GGCCGCTGCT CGACCTGCCT 
GAGTTGCCGT TCCCGGAGCT CGAGCGGGTG CTGCAAGCTC CGCCGCCGGA CGTCGGCAAC 
GGAGAAGTAC CTAAAGAATT GGTGGAGCTC CATTTGAAGT TGATGAGGAA GATTGGCAAA 
TCTGTTACAG CAGACAGATG GGAAAAATAC TTGATCAAGA TATGCCAAGA GTTTAATAGC 
ACCTGGGCGT GGGAGATGGA GAAGAAAGGC TATCTTGAAA TGAGTGTTGA ATGCAAGTTA 
GCACTCTTAA AGTACCTCTG TGAGTGTCAA TTTGATGACA ATCTCAAATT CAAGAATATT 
ATCAATGAGG AGGATGCCGA TACCATGCGT CTCCAGCCAA TTGGCCGTGA CAAAGATGGC 
CTCATGTACT GGTACCAGTT GGATCAAGAT CACAATGTCA GAATGTACAT AGAAGAACAA 
GATGATCAAG ATGGCTCTTC ATGGAAGTGC ATTGTGAGAA ATCGAAACGA GTTGGCTGAG 
ACACTTGCAC TCCTGAAAGC ACAAATTGAT CCTGTACTAT TGAAGAACTC TAGCCAACAG 
GACAGCTCTT CCCGGGAAAG TCCCAGCTTA GAAGAAGAGG AGACTAAAAA GGAGGAAGAA 
ACAACTAAAC AAGGTATTGT GTGCAGACTC TGGACAGAAA GAAGGGGAAA GGGCAGAAGG 
CATAGGTCAA CTGAACCCTG TAACAGCTGT CTTGAATCCC TTCAAGTCTG TCAAGGAACA 
TTGCTTCTGC ATTGTGATTT TGAAGAGGAA CAGAAAGGAA GTGCAAAGAT GAAAAGTGAA 
GAGCGGCCAA TAGATTCAGA AAAATGTTCT ACACCCAGTG CTCTAGAAGA GACTACTGTG 
AAAATAGAAA AAGAAGATGA AAAGGAACTT GTAAAACTGC CAGTCATAGT GAAGCTAGAA 
AAACCTTTTC CAGAAAGTGA AGAAAAAAAG ATTATCAAAG AAGAAAGTGA TTCCTTCAAG 
GAAAATGTCA AACCTGTAAA AGTGGAAATG AAAGAATGTA AAGCAGATCC TAGAGATATC 
AAAGGTAGTA TGGAGAAGTT AGAGCCTGAG AGGCTAGAGT TTGTTGGCAA TGTTAAATCT 
TCTCAAGAAA TTACTGAAAA GTCTACTGAA GAAACCGAGA AACTTAAAAA TGACCAGCAG 
GCCAAGATTC CACTAAAAAA ACGAGAAATT AAACTGAGTG ATGATTTTGA TAGTCCAATC 
AAAGGACCTT TGTGTAAATC AGTTACTCCA ACAAAAGAGT TTTTGAAAGA TGAAATAAAA 
CAAGAGGAAG AGACTTGTAA AAGGATCTCT ACAATCACTG CCTTGGGTCA TGAAGGGAAA 
CAGCTGGTAA ATGGAGAAGT TAGTGATGAA AAGGTAACTC CACATTTTAA GACAGAACAG 
ATGGAGACAA AGTTTTATGA TTCAAAGGAA GATAGTTGCA GCCCCTCTAA GGACAGAAGT 
GTCATCATGG AGGGAAATGG AGCAGAGTCT GTAAATTCTG TCATTCCAAG TGTAAAAATA 
GGTGATCTTG AGAAAGAAGT GGTCCCTTTA GGGAAAGATA CAGATAATTC AATATCAGTC 
TTAGAGACCC CGAGTCAGAA AGAGTACATA GACGAAACTG GTCCTTCAGA AATGGAAACC 
TCTCTTGAGA CTGCTGAAGT AGCAAAGGAT CTCTGTTTAA AAACTGCTTT ATCTGCCACT 
GAATCCTATA GTATGAGAGT TGAAGAGAAG TCTCCCAAAA GTAAGAAGGA TAAGCGCCCA 
CCAGTCCTAG AGTGTCTTGA AAAGTCCAAA AAGACTTTTC TTGATAAGGA CATACAAAGA 
TTGAGTCCAA TACCGGAGGA GGTTCCAAAG ACAACTGTAG AATCAGAGAA GGCTGGGTCT 
CCTGAGGCAG CTGAAACTTA TCCGTCGTCT AACGTGACTG TCCACTGTGA GAAACTAGCC 
TCAGAAGTTG AGTGTCAGAA TACAAGTTCA CTAGAAGGTC ATTCCCTGGA GAAAGTAGAC 
CCAGAAATTT TAAAAGTGGA TTCTGAATCA ACAAAGGTAG AAGTGGATAA TCTGGACAAT 
GCCCAGACCT CTGGCACAGG TGATCCTTCT GAGACAAAGG GTTCTATGCA AAAAAGCAAA 
TTGAAATATA AGTTGATTCC TGAAGAAGAA AACACTGCCT CAGAAAACAC AGAGATAACC 
TCCGAAAGGC AGAAAGAAGG CATCAAATTA ACAATTAGGA TATCTAGTCG GAAAAAGAAG 
TCAGATTCTC CTCCCAAAAT TTTAGAACCA GAAACCAAGC AAGAGAAGAC AGAAAAGGAA 
GAAGAGAAAA CAAACGTTGG TCGTACTTTG AGGAGGTCTC CAAGAATATC TAGACCCACA 
GCAAAAGTGG CTGAAATCAG AGATCAGAAA GCTGATAAAA AAAGAGGGGA AGAAGATGAA 
GTAGAAGAAG AGTCAGCAGC TTTGCAAAAG ACAGACAAAA AAGAAAATTT GAAAAAAGCA 
GAGAAGGATA CAAATTCTAA AGTAGCCAAG GTAAAGCCCA AAGGCAAAGT TCGATGGACT 
GGCTCTCGAA CACGTGGCAG GTGGAAATAT TCCAGCAATG ATGAAAGTGA AGGGTCTGAC 
AGTGAAAAAT CATCTGCAGC TTCAGAAGAG GAGGAAGAAA AGGAAAGTGA AGAAGCCATC 
CTAGCAGATG ATGATGAACC ATGCAAAAAA TGTGGCCTTC CAAACCATCC TGAATTAATC 
CTTCTGTGTG ACTCTTGTGA CAGTGGATAC CACACTGCCT GCCTTCGCCC TCCTCTAATG 
ATTATTCCAG ATGGAGAATG GTTCTGCCCC CCTTGCCAGC ATAAACTACT GTGTGAAAAA 
TTAGAAGAAC AATTGCAAGA TTTGGATGTT GCCTTAAAGA AGAAAGAGCG TGCTGAACGC 
AGGAAAGAAC GCTTGGTGTA TGTGGGTATT AGTATTGAAA ACATCATTCC TCCACAAGAG 
CCAGATTTTT CGGAAGATCA TGAAGAAAAG AAAAAAGATT CAAAAAAATC CAAAGCAAAC 
TTGCTTGAAA GGAGGTCGAC AAGAACACGG AAATGTATAA GTTACAGATT TGATGAGTTT 
GACGAAGCAA TTGATGAAGC TATAGAAGAT GATATCAAAG AGGCTGATGG AGGAGGAGTT 
GGCCGAGGAA AAGATATCTC CACCATCACA GGCCACCGTG GAAAAGACAT CTCTACTATT 
TTGGATGAAG AAAGAAAAGA AAATAAACGA CCCCAGAGGG CAGCTGCTGC TCGCCGGAAG 
AAACGCCGGC GACTGAATGA TCTGGACAGT GACAGCAACC TGGATGAAGA GGAGAGCGAG 
GATGAATTCA AGATCAGTGA TGGATCTCAG GATGAATTTG TTGTATCTGA TGAGAACCCA 
GATGAGAGTG AAGAAGACCC ACCATCTAAT GATGACAGCG ACACTGATTT CTGTAGCCGA 
AGACTAAGGC GACACCCCTC CCGGCCTATG AGGCAAAGCA GGCGTTTGCG GAGAAAGACC 
CCAAAGAGAA AGTGCTCTGA CGATGATGAG GAGGAAGAGT CTGAGGAGAA CAGCAGAGAC 
TCTGAAAGTG ACTTTAGTGA TGACTTTAGC GATGATTTTG TAGAAACTCG GCGAAGAAGG 
TCTAGAAGGA ACCAGAAGAG ACAAATTAAC TACAAGGAAG ATTCAGAAAG TGATGGCTCC 
CAGAAGAGTT TACGACGTGG TAAAGAAATC AGACGAGTTC ACAAGCGTAG GCTCTCTAGC 
TCAGATAGTG AAGAGAGCTA TATGTCCAAG AACTCTGAAG ATGATGAGCT AGCTAAAGAA 
TCAAAGCGGT CAGTTCGAAA ACGGGGTCGA AGCACAGATG AGTATTCCGA AGCAGATGAG 
GAGGAAGAAG AAGGTAAACC ATCCCGAAAA CGGCTACACC GGATCGAGAC AGATGAAGAG 
GAGAGTTGTG ACAATGCTCA TGGAGATGCA GATCAACCTG CCCATGACCG CCAGCCCAGG 
GTCCTGCCCT CAGAACAAGA CAGCACCAAA AAGCCCTATC GGATAGACAG TGACGAGGAA 
GAGGACTTTG AAAATGTAGG CAAGGTGGGG AGCCCATTGG ACTATAGCTT AGTGGACCTA 
CCTTCCACCA ATGGACAGAG TCCTGGCAAA GCCATTGAAA ACTTGATTGG CAAGCCAGCT 
GAGAAGCCTC AGACCCCCAA GGACAGCAGC ACAGCCAGTG CAAGCCTGGC CCCCAATGGG 
ACAAGTGGTG GGCAGGAGGC AGGGGCCCCA GAAGAGGACG AAGATGAGCT TTTAAGAGTG 
ACTGACCTTG TTGATTATGT CTGTAACAGT GAACAGTTAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RSF1 ( XM_010820856.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010819158.1 Bos taurus remodeling and spacing factor 1 (RSF1), transcript variant X1, mRNA.

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CloneID	OBa55069
Clone ID Related Accession (Same CDS sequence)	XM_010820856.3 , XM_010826221.2 , XM_010820856.2
Accession Version	XM_010820856.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4422bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	remodeling and spacing factor 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104633.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010820856.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG CGGCGGCAGC GGCGGCGGCG ATGGCTCCTC CGGGCTGCCC GGGTTCGTGT 
CCCAACTTCG CCGTAGTCTG CTCTTTCTTG GAGCGCTACG GGCCGCTGCT CGACCTGCCT 
GAGTTGCCGT TCCCGGAGCT CGAGCGGGTG CTGCAAGCTC CGCCGCCGGA CGTCGGCAAC 
GGAGAAGTAC CTAAAGAATT GGTGGAGCTC CATTTGAAGT TGATGAGGAA GATTGGCAAA 
TCTGTTACAG CAGACAGATG GGAAAAATAC TTGATCAAGA TATGCCAAGA GTTTAATAGC 
ACCTGGGCGT GGGAGATGGA GAAGAAAGGC TATCTTGAAA TGAGTGTTGA ATGCAAGTTA 
GCACTCTTAA AGTACCTCTG TGAGTGTCAA TTTGATGACA ATCTCAAATT CAAGAATATT 
ATCAATGAGG AGGATGCCGA TACCATGCGT CTCCAGCCAA TTGGCCGTGA CAAAGATGGC 
CTCATGTACT GGTACCAGTT GGATCAAGAT CACAATGTCA GAATGTACAT AGAAGAACAA 
GATGATCAAG ATGGCTCTTC ATGGAAGTGC ATTGTGAGAA ATCGAAACGA GTTGGCTGAG 
ACACTTGCAC TCCTGAAAGC ACAAATTGAT CCTGTACTAT TGAAGAACTC TAGCCAACAG 
GACAGCTCTT CCCGGGAAAG TCCCAGCTTA GAAGAAGAGG AGACTAAAAA GGAGGAAGAA 
ACAACTAAAC AAGGTATTGT GTGCAGACTC TGGACAGAAA GAAGGGGAAA GGGCAGAAGG 
CATAGGTCAA CTGAACCCTG TAACAGCTGT CTTGAATCCC TTCAAGTCTG TCAAGGAACA 
TTGCTTCTGC ATTGTGATTT TGAAGAGGAA CAGAAAGGAA GTGCAAAGAT GAAAAGTGAA 
GAGCGGCCAA TAGATTCAGA AAAATGTTCT ACACCCAGTG CTCTAGAAGA GACTACTGTG 
AAAATAGAAA AAGAAGATGA AAAGGAACTT GTAAAACTGC CAGTCATAGT GAAGCTAGAA 
AAACCTTTTC CAGAAAGTGA AGAAAAAAAG ATTATCAAAG AAGAAAGTGA TTCCTTCAAG 
GAAAATGTCA AACCTGTAAA AGTGGAAATG AAAGAATGTA AAGCAGATCC TAGAGATATC 
AAAGGTAGTA TGGAGAAGTT AGAGCCTGAG AGGCTAGAGT TTGTTGGCAA TGTTAAATCT 
TCTCAAGAAA TTACTGAAAA GTCTACTGAA GAAACCGAGA AACTTAAAAA TGACCAGCAG 
GCCAAGATTC CACTAAAAAA ACGAGAAATT AAACTGAGTG ATGATTTTGA TAGTCCAATC 
AAAGGACCTT TGTGTAAATC AGTTACTCCA ACAAAAGAGT TTTTGAAAGA TGAAATAAAA 
CAAGAGGAAG AGACTTGTAA AAGGATCTCT ACAATCACTG CCTTGGGTCA TGAAGGGAAA 
CAGCTGGTAA ATGGAGAAGT TAGTGATGAA AAGGTAACTC CACATTTTAA GACAGAACAG 
ATGGAGACAA AGTTTTATGA TTCAAAGGAA GATAGTTGCA GCCCCTCTAA GGACAGAAGT 
GTCATCATGG AGGGAAATGG AGCAGAGTCT GTAAATTCTG TCATTCCAAG TGTAAAAATA 
GGTGATCTTG AGAAAGAAGT GGTCCCTTTA GGGAAAGATA CAGATAATTC AATATCAGTC 
TTAGAGACCC CGAGTCAGAA AGAGTACATA GACGAAACTG GTCCTTCAGA AATGGAAACC 
TCTCTTGAGA CTGCTGAAGT AGCAAAGGAT CTCTGTTTAA AAACTGCTTT ATCTGCCACT 
GAATCCTATA GTATGAGAGT TGAAGAGAAG TCTCCCAAAA GTAAGAAGGA TAAGCGCCCA 
CCAGTCCTAG AGTGTCTTGA AAAGTCCAAA AAGACTTTTC TTGATAAGGA CATACAAAGA 
TTGAGTCCAA TACCGGAGGA GGTTCCAAAG ACAACTGTAG AATCAGAGAA GGCTGGGTCT 
CCTGAGGCAG CTGAAACTTA TCCGTCGTCT AACGTGACTG TCCACTGTGA GAAACTAGCC 
TCAGAAGTTG AGTGTCAGAA TACAAGTTCA CTAGAAGGTC ATTCCCTGGA GAAAGTAGAC 
CCAGAAATTT TAAAAGTGGA TTCTGAATCA ACAAAGGTAG AAGTGGATAA TCTGGACAAT 
GCCCAGACCT CTGGCACAGG TGATCCTTCT GAGACAAAGG GTTCTATGCA AAAAAGCAAA 
TTGAAATATA AGTTGATTCC TGAAGAAGAA AACACTGCCT CAGAAAACAC AGAGATAACC 
TCCGAAAGGC AGAAAGAAGG CATCAAATTA ACAATTAGGA TATCTAGTCG GAAAAAGAAG 
TCAGATTCTC CTCCCAAAAT TTTAGAACCA GAAACCAAGC AAGAGAAGAC AGAAAAGGAA 
GAAGAGAAAA CAAACGTTGG TCGTACTTTG AGGAGGTCTC CAAGAATATC TAGACCCACA 
GCAAAAGTGG CTGAAATCAG AGATCAGAAA GCTGATAAAA AAAGAGGGGA AGAAGATGAA 
GTAGAAGAAG AGTCAGCAGC TTTGCAAAAG ACAGACAAAA AAGAAAATTT GAAAAAAGCA 
GAGAAGGATA CAAATTCTAA AGTAGCCAAG GTAAAGCCCA AAGGCAAAGT TCGATGGACT 
GGCTCTCGAA CACGTGGCAG GTGGAAATAT TCCAGCAATG ATGAAAGTGA AGGGTCTGAC 
AGTGAAAAAT CATCTGCAGC TTCAGAAGAG GAGGAAGAAA AGGAAAGTGA AGAAGCCATC 
CTAGCAGATG ATGATGAACC ATGCAAAAAA TGTGGCCTTC CAAACCATCC TGAATTAATC 
CTTCTGTGTG ACTCTTGTGA CAGTGGATAC CACACTGCCT GCCTTCGCCC TCCTCTAATG 
ATTATTCCAG ATGGAGAATG GTTCTGCCCC CCTTGCCAGC ATAAACTACT GTGTGAAAAA 
TTAGAAGAAC AATTGCAAGA TTTGGATGTT GCCTTAAAGA AGAAAGAGCG TGCTGAACGC 
AGGAAAGAAC GCTTGGTGTA TGTGGGTATT AGTATTGAAA ACATCATTCC TCCACAAGAG 
CCAGATTTTT CGGAAGATCA TGAAGAAAAG AAAAAAGATT CAAAAAAATC CAAAGCAAAC 
TTGCTTGAAA GGAGGTCGAC AAGAACACGG AAATGTATAA GTTACAGATT TGATGAGTTT 
GACGAAGCAA TTGATGAAGC TATAGAAGAT GATATCAAAG AGGCTGATGG AGGAGGAGTT 
GGCCGAGGAA AAGATATCTC CACCATCACA GGCCACCGTG GAAAAGACAT CTCTACTATT 
TTGGATGAAG AAAGAAAAGA AAATAAACGA CCCCAGAGGG CAGCTGCTGC TCGCCGGAAG 
AAACGCCGGC GACTGAATGA TCTGGACAGT GACAGCAACC TGGATGAAGA GGAGAGCGAG 
GATGAATTCA AGATCAGTGA TGGATCTCAG GATGAATTTG TTGTATCTGA TGAGAACCCA 
GATGAGAGTG AAGAAGACCC ACCATCTAAT GATGACAGCG ACACTGATTT CTGTAGCCGA 
AGACTAAGGC GACACCCCTC CCGGCCTATG AGGCAAAGCA GGCGTTTGCG GAGAAAGACC 
CCAAAGAGAA AGTGCTCTGA CGATGATGAG GAGGAAGAGT CTGAGGAGAA CAGCAGAGAC 
TCTGAAAGTG ACTTTAGTGA TGACTTTAGC GATGATTTTG TAGAAACTCG GCGAAGAAGG 
TCTAGAAGGA ACCAGAAGAG ACAAATTAAC TACAAGGAAG ATTCAGAAAG TGATGGCTCC 
CAGAAGAGTT TACGACGTGG TAAAGAAATC AGACGAGTTC ACAAGCGTAG GCTCTCTAGC 
TCAGATAGTG AAGAGAGCTA TATGTCCAAG AACTCTGAAG ATGATGAGCT AGCTAAAGAA 
TCAAAGCGGT CAGTTCGAAA ACGGGGTCGA AGCACAGATG AGTATTCCGA AGCAGATGAG 
GAGGAAGAAG AAGGTAAACC ATCCCGAAAA CGGCTACACC GGATCGAGAC AGATGAAGAG 
GAGAGTTGTG ACAATGCTCA TGGAGATGCA GATCAACCTG CCCATGACCG CCAGCCCAGG 
GTCCTGCCCT CAGAACAAGA CAGCACCAAA AAGCCCTATC GGATAGACAG TGACGAGGAA 
GAGGACTTTG AAAATGTAGG CAAGGTGGGG AGCCCATTGG ACTATAGCTT AGTGGACCTA 
CCTTCCACCA ATGGACAGAG TCCTGGCAAA GCCATTGAAA ACTTGATTGG CAAGCCAGCT 
GAGAAGCCTC AGACCCCCAA GGACAGCAGC ACAGCCAGTG CAAGCCTGGC CCCCAATGGG 
ACAAGTGGTG GGCAGGAGGC AGGGGCCCCA GAAGAGGACG AAGATGAGCT TTTAAGAGTG 
ACTGACCTTG TTGATTATGT CTGTAACAGT GAACAGTTAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010819158.1
CDS	284..4705
Translation	MAAAAAAAAAMAPPGCPGSCPNFAVVCSFLERYGPLLDLPELPFPELERVLQAPPPDVGNGEVPKELVELHLKLMRKIGKSVTADRWEKYLIKICQEFNSTWAWEMEKKGYLEMSVECKLALLKYLCECQFDDNLKFKNIINEEDADTMRLQPIGRDKDGLMYWYQLDQDHNVRMYIEEQDDQDGSSWKCIVRNRNELAETLALLKAQIDPVLLKNSSQQDSSSRESPSLEEEETKKEEETTKQGIVCRLWTERRGKGRRHRSTEPCNSCLESLQVCQGTLLLHCDFEEEQKGSAKMKSEERPIDSEKCSTPSALEETTVKIEKEDEKELVKLPVIVKLEKPFPESEEKKIIKEESDSFKENVKPVKVEMKECKADPRDIKGSMEKLEPERLEFVGNVKSSQEITEKSTEETEKLKNDQQAKIPLKKREIKLSDDFDSPIKGPLCKSVTPTKEFLKDEIKQEEETCKRISTITALGHEGKQLVNGEVSDEKVTPHFKTEQMETKFYDSKEDSCSPSKDRSVIMEGNGAESVNSVIPSVKIGDLEKEVVPLGKDTDNSISVLETPSQKEYIDETGPSEMETSLETAEVAKDLCLKTALSATESYSMRVEEKSPKSKKDKRPPVLECLEKSKKTFLDKDIQRLSPIPEEVPKTTVESEKAGSPEAAETYPSSNVTVHCEKLASEVECQNTSSLEGHSLEKVDPEILKVDSESTKVEVDNLDNAQTSGTGDPSETKGSMQKSKLKYKLIPEEENTASENTEITSERQKEGIKLTIRISSRKKKSDSPPKILEPETKQEKTEKEEEKTNVGRTLRRSPRISRPTAKVAEIRDQKADKKRGEEDEVEEESAALQKTDKKENLKKAEKDTNSKVAKVKPKGKVRWTGSRTRGRWKYSSNDESEGSDSEKSSAASEEEEEKESEEAILADDDEPCKKCGLPNHPELILLCDSCDSGYHTACLRPPLMIIPDGEWFCPPCQHKLLCEKLEEQLQDLDVALKKKERAERRKERLVYVGISIENIIPPQEPDFSEDHEEKKKDSKKSKANLLERRSTRTRKCISYRFDEFDEAIDEAIEDDIKEADGGGVGRGKDISTITGHRGKDISTILDEERKENKRPQRAAAARRKKRRRLNDLDSDSNLDEEESEDEFKISDGSQDEFVVSDENPDESEEDPPSNDDSDTDFCSRRLRRHPSRPMRQSRRLRRKTPKRKCSDDDEEEESEENSRDSESDFSDDFSDDFVETRRRRSRRNQKRQINYKEDSESDGSQKSLRRGKEIRRVHKRRLSSSDSEESYMSKNSEDDELAKESKRSVRKRGRSTDEYSEADEEEEEGKPSRKRLHRIETDEEESCDNAHGDADQPAHDRQPRVLPSEQDSTKKPYRIDSDEEEDFENVGKVGSPLDYSLVDLPSTNGQSPGKAIENLIGKPAEKPQTPKDSSTASASLAPNGTSGGQEAGAPEEDEDELLRVTDLVDYVCNSEQL

Target ORF information:

RefSeq Version	XM_010820856.2
Organism	Bos taurus(cattle)
Definition	Bos taurus remodeling and spacing factor 1 (RSF1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010820856.2

ORF Insert Sequence:

ATGGCGGCGG CGGCGGCAGC GGCGGCGGCG ATGGCTCCTC CGGGCTGCCC GGGTTCGTGT 
CCCAACTTCG CCGTAGTCTG CTCTTTCTTG GAGCGCTACG GGCCGCTGCT CGACCTGCCT 
GAGTTGCCGT TCCCGGAGCT CGAGCGGGTG CTGCAAGCTC CGCCGCCGGA CGTCGGCAAC 
GGAGAAGTAC CTAAAGAATT GGTGGAGCTC CATTTGAAGT TGATGAGGAA GATTGGCAAA 
TCTGTTACAG CAGACAGATG GGAAAAATAC TTGATCAAGA TATGCCAAGA GTTTAATAGC 
ACCTGGGCGT GGGAGATGGA GAAGAAAGGC TATCTTGAAA TGAGTGTTGA ATGCAAGTTA 
GCACTCTTAA AGTACCTCTG TGAGTGTCAA TTTGATGACA ATCTCAAATT CAAGAATATT 
ATCAATGAGG AGGATGCCGA TACCATGCGT CTCCAGCCAA TTGGCCGTGA CAAAGATGGC 
CTCATGTACT GGTACCAGTT GGATCAAGAT CACAATGTCA GAATGTACAT AGAAGAACAA 
GATGATCAAG ATGGCTCTTC ATGGAAGTGC ATTGTGAGAA ATCGAAACGA GTTGGCTGAG 
ACACTTGCAC TCCTGAAAGC ACAAATTGAT CCTGTACTAT TGAAGAACTC TAGCCAACAG 
GACAGCTCTT CCCGGGAAAG TCCCAGCTTA GAAGAAGAGG AGACTAAAAA GGAGGAAGAA 
ACAACTAAAC AAGGTATTGT GTGCAGACTC TGGACAGAAA GAAGGGGAAA GGGCAGAAGG 
CATAGGTCAA CTGAACCCTG TAACAGCTGT CTTGAATCCC TTCAAGTCTG TCAAGGAACA 
TTGCTTCTGC ATTGTGATTT TGAAGAGGAA CAGAAAGGAA GTGCAAAGAT GAAAAGTGAA 
GAGCGGCCAA TAGATTCAGA AAAATGTTCT ACACCCAGTG CTCTAGAAGA GACTACTGTG 
AAAATAGAAA AAGAAGATGA AAAGGAACTT GTAAAACTGC CAGTCATAGT GAAGCTAGAA 
AAACCTTTTC CAGAAAGTGA AGAAAAAAAG ATTATCAAAG AAGAAAGTGA TTCCTTCAAG 
GAAAATGTCA AACCTGTAAA AGTGGAAATG AAAGAATGTA AAGCAGATCC TAGAGATATC 
AAAGGTAGTA TGGAGAAGTT AGAGCCTGAG AGGCTAGAGT TTGTTGGCAA TGTTAAATCT 
TCTCAAGAAA TTACTGAAAA GTCTACTGAA GAAACCGAGA AACTTAAAAA TGACCAGCAG 
GCCAAGATTC CACTAAAAAA ACGAGAAATT AAACTGAGTG ATGATTTTGA TAGTCCAATC 
AAAGGACCTT TGTGTAAATC AGTTACTCCA ACAAAAGAGT TTTTGAAAGA TGAAATAAAA 
CAAGAGGAAG AGACTTGTAA AAGGATCTCT ACAATCACTG CCTTGGGTCA TGAAGGGAAA 
CAGCTGGTAA ATGGAGAAGT TAGTGATGAA AAGGTAACTC CACATTTTAA GACAGAACAG 
ATGGAGACAA AGTTTTATGA TTCAAAGGAA GATAGTTGCA GCCCCTCTAA GGACAGAAGT 
GTCATCATGG AGGGAAATGG AGCAGAGTCT GTAAATTCTG TCATTCCAAG TGTAAAAATA 
GGTGATCTTG AGAAAGAAGT GGTCCCTTTA GGGAAAGATA CAGATAATTC AATATCAGTC 
TTAGAGACCC CGAGTCAGAA AGAGTACATA GACGAAACTG GTCCTTCAGA AATGGAAACC 
TCTCTTGAGA CTGCTGAAGT AGCAAAGGAT CTCTGTTTAA AAACTGCTTT ATCTGCCACT 
GAATCCTATA GTATGAGAGT TGAAGAGAAG TCTCCCAAAA GTAAGAAGGA TAAGCGCCCA 
CCAGTCCTAG AGTGTCTTGA AAAGTCCAAA AAGACTTTTC TTGATAAGGA CATACAAAGA 
TTGAGTCCAA TACCGGAGGA GGTTCCAAAG ACAACTGTAG AATCAGAGAA GGCTGGGTCT 
CCTGAGGCAG CTGAAACTTA TCCGTCGTCT AACGTGACTG TCCACTGTGA GAAACTAGCC 
TCAGAAGTTG AGTGTCAGAA TACAAGTTCA CTAGAAGGTC ATTCCCTGGA GAAAGTAGAC 
CCAGAAATTT TAAAAGTGGA TTCTGAATCA ACAAAGGTAG AAGTGGATAA TCTGGACAAT 
GCCCAGACCT CTGGCACAGG TGATCCTTCT GAGACAAAGG GTTCTATGCA AAAAAGCAAA 
TTGAAATATA AGTTGATTCC TGAAGAAGAA AACACTGCCT CAGAAAACAC AGAGATAACC 
TCCGAAAGGC AGAAAGAAGG CATCAAATTA ACAATTAGGA TATCTAGTCG GAAAAAGAAG 
TCAGATTCTC CTCCCAAAAT TTTAGAACCA GAAACCAAGC AAGAGAAGAC AGAAAAGGAA 
GAAGAGAAAA CAAACGTTGG TCGTACTTTG AGGAGGTCTC CAAGAATATC TAGACCCACA 
GCAAAAGTGG CTGAAATCAG AGATCAGAAA GCTGATAAAA AAAGAGGGGA AGAAGATGAA 
GTAGAAGAAG AGTCAGCAGC TTTGCAAAAG ACAGACAAAA AAGAAAATTT GAAAAAAGCA 
GAGAAGGATA CAAATTCTAA AGTAGCCAAG GTAAAGCCCA AAGGCAAAGT TCGATGGACT 
GGCTCTCGAA CACGTGGCAG GTGGAAATAT TCCAGCAATG ATGAAAGTGA AGGGTCTGAC 
AGTGAAAAAT CATCTGCAGC TTCAGAAGAG GAGGAAGAAA AGGAAAGTGA AGAAGCCATC 
CTAGCAGATG ATGATGAACC ATGCAAAAAA TGTGGCCTTC CAAACCATCC TGAATTAATC 
CTTCTGTGTG ACTCTTGTGA CAGTGGATAC CACACTGCCT GCCTTCGCCC TCCTCTAATG 
ATTATTCCAG ATGGAGAATG GTTCTGCCCC CCTTGCCAGC ATAAACTACT GTGTGAAAAA 
TTAGAAGAAC AATTGCAAGA TTTGGATGTT GCCTTAAAGA AGAAAGAGCG TGCTGAACGC 
AGGAAAGAAC GCTTGGTGTA TGTGGGTATT AGTATTGAAA ACATCATTCC TCCACAAGAG 
CCAGATTTTT CGGAAGATCA TGAAGAAAAG AAAAAAGATT CAAAAAAATC CAAAGCAAAC 
TTGCTTGAAA GGAGGTCGAC AAGAACACGG AAATGTATAA GTTACAGATT TGATGAGTTT 
GACGAAGCAA TTGATGAAGC TATAGAAGAT GATATCAAAG AGGCTGATGG AGGAGGAGTT 
GGCCGAGGAA AAGATATCTC CACCATCACA GGCCACCGTG GAAAAGACAT CTCTACTATT 
TTGGATGAAG AAAGAAAAGA AAATAAACGA CCCCAGAGGG CAGCTGCTGC TCGCCGGAAG 
AAACGCCGGC GACTGAATGA TCTGGACAGT GACAGCAACC TGGATGAAGA GGAGAGCGAG 
GATGAATTCA AGATCAGTGA TGGATCTCAG GATGAATTTG TTGTATCTGA TGAGAACCCA 
GATGAGAGTG AAGAAGACCC ACCATCTAAT GATGACAGCG ACACTGATTT CTGTAGCCGA 
AGACTAAGGC GACACCCCTC CCGGCCTATG AGGCAAAGCA GGCGTTTGCG GAGAAAGACC 
CCAAAGAGAA AGTGCTCTGA CGATGATGAG GAGGAAGAGT CTGAGGAGAA CAGCAGAGAC 
TCTGAAAGTG ACTTTAGTGA TGACTTTAGC GATGATTTTG TAGAAACTCG GCGAAGAAGG 
TCTAGAAGGA ACCAGAAGAG ACAAATTAAC TACAAGGAAG ATTCAGAAAG TGATGGCTCC 
CAGAAGAGTT TACGACGTGG TAAAGAAATC AGACGAGTTC ACAAGCGTAG GCTCTCTAGC 
TCAGATAGTG AAGAGAGCTA TATGTCCAAG AACTCTGAAG ATGATGAGCT AGCTAAAGAA 
TCAAAGCGGT CAGTTCGAAA ACGGGGTCGA AGCACAGATG AGTATTCCGA AGCAGATGAG 
GAGGAAGAAG AAGGTAAACC ATCCCGAAAA CGGCTACACC GGATCGAGAC AGATGAAGAG 
GAGAGTTGTG ACAATGCTCA TGGAGATGCA GATCAACCTG CCCATGACCG CCAGCCCAGG 
GTCCTGCCCT CAGAACAAGA CAGCACCAAA AAGCCCTATC GGATAGACAG TGACGAGGAA 
GAGGACTTTG AAAATGTAGG CAAGGTGGGG AGCCCATTGG ACTATAGCTT AGTGGACCTA 
CCTTCCACCA ATGGACAGAG TCCTGGCAAA GCCATTGAAA ACTTGATTGG CAAGCCAGCT 
GAGAAGCCTC AGACCCCCAA GGACAGCAGC ACAGCCAGTG CAAGCCTGGC CCCCAATGGG 
ACAAGTGGTG GGCAGGAGGC AGGGGCCCCA GAAGAGGACG AAGATGAGCT TTTAAGAGTG 
ACTGACCTTG TTGATTATGT CTGTAACAGT GAACAGTTAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	RSF1
Entrez Gene ID	541248
Full Name	remodeling and spacing factor 1
General protein information	Preferred Names remodeling and spacing factor 1 Names remodeling and spacing factor 1
Gene Type	protein-coding
Organism	Bos taurus(cattle)

	RSF1	XP_001091378.1
Canis lupus familiaris (dog)	RSF1	XP_534002.2
Rattus norvegicus (Norway rat)	Rsf1	XP_218939.4
Homo sapiens (human)	RSF1	NP_057662.3
Gallus gallus (chicken)	RSF1	XP_424124.3
Danio rerio (zebrafish)	si:dkey-21n12.1	XP_005169237.1
Bos taurus (cattle)	RSF1	XP_002699109.1
Mus musculus (house mouse)	Rsf1	NP_001074736.1
Pan troglodytes (chimpanzee)	RSF1	XP_001175061.2

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

RSF1 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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