NDUFB9 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	NDUFB9
Entrez Gene ID	327660
Full Name	NADH:ubiquinone oxidoreductase subunit B9
Synonyms	B22
General protein information	Preferred Names NADH:ubiquinone oxidoreductase subunit B9 Names NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 CI-B22 NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 9, 22kDa NADH-ubiquinone oxidoreductase B22 subunit complex I-B22
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005215352.4	XP_005215409.2	NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 isoform X1
XM_005215352.3	XP_005215409.2	NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 isoform X1
XM_005215351.3	XP_005215408.1	NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 isoform X2
NM_175783.4	NP_786977.1	NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	NDUFB9	NP_001065267.1
Canis lupus familiaris (dog)	NDUFB9	XP_005627982.1
Rattus norvegicus (Norway rat)	Ndufb9	NP_001120766.1
Bos taurus (cattle)	NDUFB9	NP_786977.1
Mus musculus (house mouse)	Ndufb9	NP_075661.1
	LOC711387	XP_001100168.2
Gallus gallus (chicken)	NDUFB9	XP_418444.3
Danio rerio (zebrafish)	ndufb9	NP_001017880.1
Drosophila melanogaster (fruit fly)	CG9306	NP_609642.1
Caenorhabditis elegans (roundworm)	C16A3.5	NP_498396.1
Homo sapiens (human)	NDUFB9	NP_004996.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009865	XP_318983.3
Xenopus tropicalis (tropical clawed frog)	ndufb9	NP_001072252.1

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

Subunit analysis of bovine heart complex I by reversed-phase high-performance liquid chromatography, electrospray ionization-tandem mass spectrometry, and matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry.
Lemma-Gray P, Valusov? E, Carroll CA, Weintraub ST, Musatov A, Robinson NC
Analytical biochemistry382(2)116-21(2008 Nov)

Resolution of the membrane domain of bovine complex I into subcomplexes: implications for the structural organization of the enzyme.
Sazanov LA, Peak-Chew SY, Fearnley IM, Walker JE
Biochemistry39(24)7229-35(2000 Jun)

Sequences of 20 subunits of NADH:ubiquinone oxidoreductase from bovine heart mitochondria. Application of a novel strategy for sequencing proteins using the polymerase chain reaction.
Walker JE, Arizmendi JM, Dupuis A, Fearnley IM, Finel M, Medd SM, Pilkington SJ, Runswick MJ, Skehel JM
Journal of molecular biology226(4)1051-72(1992 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following NDUFB9 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NDUFB9 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa00008	NM_175783.4 Latest version!	Bos taurus NADH:ubiquinone oxidoreductase subunit B9 (NDUFB9), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OBa25880	XM_005215351.3 Latest version!	Bos taurus NADH:ubiquinone oxidoreductase subunit B9 (NDUFB9), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OBa45554	XM_005215352.4 Latest version!	Bos taurus NADH:ubiquinone oxidoreductase subunit B9 (NDUFB9), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
View Old Accession Versions >>
OBa45554	XM_005215352.3	Bos taurus NADH:ubiquinone oxidoreductase subunit B9 (NDUFB9), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NDUFB9 ( NM_175783.4 ) cDNA ORF clone, Bos taurus(cattle) -> NP_786977.1 Bos taurus NADH:ubiquinone oxidoreductase subunit B9 (NDUFB9), mRNA.

Price & Availability↑

CloneID	OBa00008
Clone ID Related Accession (Same CDS sequence)	NM_175783.4
Accession Version	NM_175783.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 540bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Bos taurus(cattle)
Product	NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC102701.1. On Aug 31, 2012 this sequence version replaced NM_175783.3. ##Evidence-Data-START## Transcript exon combination :: BC102701.1, EV665145.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145412, SAMN03145413 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

ATGGCATTTT TGTCGTCGGG GGCCTATCTG ACCCACCAGC AGAAGGTGCT GCGGCTTTAT 
AAGCGGGCGC TGCGACACCT GGAATCATGG TGCATCCACA GGGATAAATA CCGGTACTTT 
GCTTGCTTGT TGAGAGCCCG GTTTGATGAA CATAAGAATG AAAAGGACAT GGTGAAGGCC 
ACCCAGCTGC TGAGGGAGGC AGAGGAAGAA TTCTGGCATG GTCAGCACCC CCAGCCGTAC 
ATCTTCCCTG AGTCTCCAGG GGGCACCTCC TATGAGAGAT ATGAGTGTTA CAAGGTTCCC 
GAGTGGTGCT TAGATGACTG GCATCCTTCG GAGAAGGCGA TGTATCCTGA CTACTTTGCT 
AAGAGAGAGC AATGGAAGAA ACTGCGGAGG GAGAGCTGGG AGCGGGAGGT GAAGCAGCTG 
CAGGAGGAAA CCCCAGTTGG TGGTCCCCGA ACTGAAGCTT TGCCCCCAGC CCGAAAGCAA 
GGTGACTTGC CCCCTCTGTG GTGGCATATC GTGACCAGAC CCCGGGAGCG GCCCATGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_786977.1
CDS	38..577
Translation	MAFLSSGAYLTHQQKVLRLYKRALRHLESWCIHRDKYRYFACLLRARFDEHKNEKDMVKATQLLREAEEEFWHGQHPQPYIFPESPGGTSYERYECYKVPEWCLDDWHPSEKAMYPDYFAKREQWKKLRRESWEREVKQLQEETPVGGPRTEALPPARKQGDLPPLWWHIVTRPRERPM

Target ORF information:

RefSeq Version	NM_175783.4
Organism	Bos taurus(cattle)
Definition	Bos taurus NADH:ubiquinone oxidoreductase subunit B9 (NDUFB9), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_175783.4

ORF Insert Sequence:

ATGGCATTTT TGTCGTCGGG GGCCTATCTG ACCCACCAGC AGAAGGTGCT GCGGCTTTAT 
AAGCGGGCGC TGCGACACCT GGAATCATGG TGCATCCACA GGGATAAATA CCGGTACTTT 
GCTTGCTTGT TGAGAGCCCG GTTTGATGAA CATAAGAATG AAAAGGACAT GGTGAAGGCC 
ACCCAGCTGC TGAGGGAGGC AGAGGAAGAA TTCTGGCATG GTCAGCACCC CCAGCCGTAC 
ATCTTCCCTG AGTCTCCAGG GGGCACCTCC TATGAGAGAT ATGAGTGTTA CAAGGTTCCC 
GAGTGGTGCT TAGATGACTG GCATCCTTCG GAGAAGGCGA TGTATCCTGA CTACTTTGCT 
AAGAGAGAGC AATGGAAGAA ACTGCGGAGG GAGAGCTGGG AGCGGGAGGT GAAGCAGCTG 
CAGGAGGAAA CCCCAGTTGG TGGTCCCCGA ACTGAAGCTT TGCCCCCAGC CCGAAAGCAA 
GGTGACTTGC CCCCTCTGTG GTGGCATATC GTGACCAGAC CCCGGGAGCG GCCCATGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NDUFB9 ( XM_005215351.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005215408.1 Bos taurus NADH:ubiquinone oxidoreductase subunit B9 (NDUFB9), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa25880
Clone ID Related Accession (Same CDS sequence)	XM_005215351.3
Accession Version	XM_005215351.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 372bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037341.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005215351.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGAAGG CCACCCAGCT GCTGAGGGAG GCAGAGGAAG AATTCTGGCA TGGTCAGCAC 
CCCCAGCCGT ACATCTTCCC TGAGTCTCCA GGGGGCACCT CCTATGAGAG ATATGAGTGT 
TACAAGGTTC CCGAGTGGTG CTTAGATGAC TGGCATCCTT CGGAGAAGGC GATGTATCCT 
GACTACTTTG CTAAGAGAGA GCAATGGAAG AAACTGCGGA GGGAGAGCTG GGAGCGGGAG 
GTGAAGCAGC TGCAGGAGGA AACCCCAGTT GGTGGTCCCC GAACTGAAGC TTTGCCCCCA 
GCCCGAAAGC AAGGTGACTT GCCCCCTCTG TGGTGGCATA TCGTGACCAG ACCCCGGGAG 
CGGCCCATGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005215408.1
CDS	288..659
Translation	MVKATQLLREAEEEFWHGQHPQPYIFPESPGGTSYERYECYKVPEWCLDDWHPSEKAMYPDYFAKREQWKKLRRESWEREVKQLQEETPVGGPRTEALPPARKQGDLPPLWWHIVTRPRERPM

Target ORF information:

RefSeq Version	XM_005215351.3
Organism	Bos taurus(cattle)
Definition	Bos taurus NADH:ubiquinone oxidoreductase subunit B9 (NDUFB9), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005215351.3

ORF Insert Sequence:

ATGGTGAAGG CCACCCAGCT GCTGAGGGAG GCAGAGGAAG AATTCTGGCA TGGTCAGCAC 
CCCCAGCCGT ACATCTTCCC TGAGTCTCCA GGGGGCACCT CCTATGAGAG ATATGAGTGT 
TACAAGGTTC CCGAGTGGTG CTTAGATGAC TGGCATCCTT CGGAGAAGGC GATGTATCCT 
GACTACTTTG CTAAGAGAGA GCAATGGAAG AAACTGCGGA GGGAGAGCTG GGAGCGGGAG 
GTGAAGCAGC TGCAGGAGGA AACCCCAGTT GGTGGTCCCC GAACTGAAGC TTTGCCCCCA 
GCCCGAAAGC AAGGTGACTT GCCCCCTCTG TGGTGGCATA TCGTGACCAG ACCCCGGGAG 
CGGCCCATGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NDUFB9 ( XM_005215352.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005215409.2 Bos taurus NADH:ubiquinone oxidoreductase subunit B9 (NDUFB9), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa45554
Clone ID Related Accession (Same CDS sequence)	XM_005215352.4 , XM_005215352.3
Accession Version	XM_005215352.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 594bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037341.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005215352.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCATC CACAGGAAAA CGGACGCGGT ACCCACGCAG AGGGTTGCAT AACAAGAGAA 
AGAAAGTACC ACAGATGGAC TGTGTTGTGC CTCCAGCGCT CACTCAATAA TAGCTTCCTT 
CGTGACCGCG TTCCTAATAT TGGCAGTCAT AACAAGGATA AATACCGGTA CTTTGCTTGC 
TTGTTGAGAG CCCGGTTTGA TGAACATAAG AATGAAAAGG ACATGGTGAA GGCCACCCAG 
CTGCTGAGGG AGGCAGAGGA AGAATTCTGG CATGGTCAGC ACCCCCAGCC GTACATCTTC 
CCTGAGTCTC CAGGGGGCAC CTCCTATGAG AGATATGAGT GTTACAAGGT TCCCGAGTGG 
TGCTTAGATG ACTGGCATCC TTCGGAGAAG GCGATGTATC CTGACTACTT TGCTAAGAGA 
GAGCAATGGA AGAAACTGCG GAGGGAGAGC TGGGAGCGGG AGGTGAAGCA GCTGCAGGAG 
GAAACCCCAG TTGGTGGTCC CCGAACTGAA GCTTTGCCCC CAGCCCGAAA GCAAGGTGAC 
TTGCCCCCTC TGTGGTGGCA TATCGTGACC AGACCCCGGG AGCGGCCCAT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005215409.2
CDS	146..739
Translation	MVHPQENGRGTHAEGCITRERKYHRWTVLCLQRSLNNSFLRDRVPNIGSHNKDKYRYFACLLRARFDEHKNEKDMVKATQLLREAEEEFWHGQHPQPYIFPESPGGTSYERYECYKVPEWCLDDWHPSEKAMYPDYFAKREQWKKLRRESWEREVKQLQEETPVGGPRTEALPPARKQGDLPPLWWHIVTRPRERPM

Target ORF information:

RefSeq Version	XM_005215352.4
Organism	Bos taurus(cattle)
Definition	Bos taurus NADH:ubiquinone oxidoreductase subunit B9 (NDUFB9), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005215352.4

ORF Insert Sequence:

ATGGTGCATC CACAGGAAAA CGGACGCGGT ACCCACGCAG AGGGTTGCAT AACAAGAGAA 
AGAAAGTACC ACAGATGGAC TGTGTTGTGC CTCCAGCGCT CACTCAATAA TAGCTTCCTT 
CGTGACCGCG TTCCTAATAT TGGCAGTCAT AACAAGGATA AATACCGGTA CTTTGCTTGC 
TTGTTGAGAG CCCGGTTTGA TGAACATAAG AATGAAAAGG ACATGGTGAA GGCCACCCAG 
CTGCTGAGGG AGGCAGAGGA AGAATTCTGG CATGGTCAGC ACCCCCAGCC GTACATCTTC 
CCTGAGTCTC CAGGGGGCAC CTCCTATGAG AGATATGAGT GTTACAAGGT TCCCGAGTGG 
TGCTTAGATG ACTGGCATCC TTCGGAGAAG GCGATGTATC CTGACTACTT TGCTAAGAGA 
GAGCAATGGA AGAAACTGCG GAGGGAGAGC TGGGAGCGGG AGGTGAAGCA GCTGCAGGAG 
GAAACCCCAG TTGGTGGTCC CCGAACTGAA GCTTTGCCCC CAGCCCGAAA GCAAGGTGAC 
TTGCCCCCTC TGTGGTGGCA TATCGTGACC AGACCCCGGG AGCGGCCCAT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NDUFB9 ( XM_005215352.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005215409.2 Bos taurus NADH:ubiquinone oxidoreductase subunit B9 (NDUFB9), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa45554
Clone ID Related Accession (Same CDS sequence)	XM_005215352.4 , XM_005215352.3
Accession Version	XM_005215352.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 594bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104392.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005215352.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCATC CACAGGAAAA CGGACGCGGT ACCCACGCAG AGGGTTGCAT AACAAGAGAA 
AGAAAGTACC ACAGATGGAC TGTGTTGTGC CTCCAGCGCT CACTCAATAA TAGCTTCCTT 
CGTGACCGCG TTCCTAATAT TGGCAGTCAT AACAAGGATA AATACCGGTA CTTTGCTTGC 
TTGTTGAGAG CCCGGTTTGA TGAACATAAG AATGAAAAGG ACATGGTGAA GGCCACCCAG 
CTGCTGAGGG AGGCAGAGGA AGAATTCTGG CATGGTCAGC ACCCCCAGCC GTACATCTTC 
CCTGAGTCTC CAGGGGGCAC CTCCTATGAG AGATATGAGT GTTACAAGGT TCCCGAGTGG 
TGCTTAGATG ACTGGCATCC TTCGGAGAAG GCGATGTATC CTGACTACTT TGCTAAGAGA 
GAGCAATGGA AGAAACTGCG GAGGGAGAGC TGGGAGCGGG AGGTGAAGCA GCTGCAGGAG 
GAAACCCCAG TTGGTGGTCC CCGAACTGAA GCTTTGCCCC CAGCCCGAAA GCAAGGTGAC 
TTGCCCCCTC TGTGGTGGCA TATCGTGACC AGACCCCGGG AGCGGCCCAT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005215409.2
CDS	117..710
Translation	MVHPQENGRGTHAEGCITRERKYHRWTVLCLQRSLNNSFLRDRVPNIGSHNKDKYRYFACLLRARFDEHKNEKDMVKATQLLREAEEEFWHGQHPQPYIFPESPGGTSYERYECYKVPEWCLDDWHPSEKAMYPDYFAKREQWKKLRRESWEREVKQLQEETPVGGPRTEALPPARKQGDLPPLWWHIVTRPRERPM

Target ORF information:

RefSeq Version	XM_005215352.3
Organism	Bos taurus(cattle)
Definition	Bos taurus NADH:ubiquinone oxidoreductase subunit B9 (NDUFB9), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005215352.3

ORF Insert Sequence:

ATGGTGCATC CACAGGAAAA CGGACGCGGT ACCCACGCAG AGGGTTGCAT AACAAGAGAA 
AGAAAGTACC ACAGATGGAC TGTGTTGTGC CTCCAGCGCT CACTCAATAA TAGCTTCCTT 
CGTGACCGCG TTCCTAATAT TGGCAGTCAT AACAAGGATA AATACCGGTA CTTTGCTTGC 
TTGTTGAGAG CCCGGTTTGA TGAACATAAG AATGAAAAGG ACATGGTGAA GGCCACCCAG 
CTGCTGAGGG AGGCAGAGGA AGAATTCTGG CATGGTCAGC ACCCCCAGCC GTACATCTTC 
CCTGAGTCTC CAGGGGGCAC CTCCTATGAG AGATATGAGT GTTACAAGGT TCCCGAGTGG 
TGCTTAGATG ACTGGCATCC TTCGGAGAAG GCGATGTATC CTGACTACTT TGCTAAGAGA 
GAGCAATGGA AGAAACTGCG GAGGGAGAGC TGGGAGCGGG AGGTGAAGCA GCTGCAGGAG 
GAAACCCCAG TTGGTGGTCC CCGAACTGAA GCTTTGCCCC CAGCCCGAAA GCAAGGTGAC 
TTGCCCCCTC TGTGGTGGCA TATCGTGACC AGACCCCGGG AGCGGCCCAT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

Subunit analysis of bovine heart complex I by reversed-phase high-performance liquid chromatography, electrospray ionization-tandem mass spectrometry, and matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry.
Analytical biochemistry382(2)116-21(2008 Nov)
Lemma-Gray P,Valusov? E,Carroll CA,Weintraub ST,Musatov A,Robinson NC

Resolution of the membrane domain of bovine complex I into subcomplexes: implications for the structural organization of the enzyme.
Biochemistry39(24)7229-35(2000 Jun)
Sazanov LA,Peak-Chew SY,Fearnley IM,Walker JE

Sequences of 20 subunits of NADH:ubiquinone oxidoreductase from bovine heart mitochondria. Application of a novel strategy for sequencing proteins using the polymerase chain reaction.
Journal of molecular biology226(4)1051-72(1992 Aug)
Walker JE,Arizmendi JM,Dupuis A,Fearnley IM,Finel M,Medd SM,Pilkington SJ,Runswick MJ,Skehel JM