CAPN3 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	CAPN3
Entrez Gene ID	281663
Full Name	calpain 3
Synonyms	SKM-CALP
General protein information	Preferred Names calpain 3 Names calpain-3 CANP 3 calcium-activated neutral proteinase 3 calpain 3, (p94) calpain L3 calpain p94 muscle-specific calcium-activated neutral protease 3 muscle-specific calpain nCL-1 new calpain 1 skeletal muscle-specific calpain
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_024997263.1	XP_024853031.1	calpain-3 isoform X1
XM_024997264.1	XP_024853032.1	calpain-3 isoform X3
XM_024997265.1	XP_024853033.1	calpain-3 isoform X4
NM_174260.2	NP_776685.1	calpain-3
XM_015473111.1	XP_015328597.1	calpain-3 isoform X1
XM_015473111.2	XP_015328597.1	calpain-3 isoform X2

Pathways from BioSystems

Homologies

Xenopus tropicalis (tropical clawed frog)	LOC100497532	XP_002935381.2
Homo sapiens (human)	CAPN3	NP_000061.1
Mus musculus (house mouse)	Capn3	NP_031627.2
Rattus norvegicus (Norway rat)	Capn3	NP_058813.1
Canis lupus familiaris (dog)	CAPN3	XP_005638349.1
Gallus gallus (chicken)	CAPN3	NP_001004405.1
	CAPN3	XP_001103220.1
Danio rerio (zebrafish)	capn3a	NP_001004571.1
Bos taurus (cattle)	CAPN3	NP_776685.1

Gene Ontology
Bibliography

Related articles in PubMed

Characterization of a novel CAPN3 transcript generated by alternative splicing in cattle.
Liu SY, Jiang H, Yuan B, Gao Y, Dai LS, Zhang JB
Genetics and molecular research : GMR14(1)457-63(2015 Jan)

Production and processing studies on calpain-system gene markers for beef tenderness: consumer assessments of eating quality.
Robinson DL, Cafe LM, McIntyre BL, Geesink GH, Barendse W, Pethick DW, Thompson JM, Polkinghorne R, Greenwood PL
Journal of animal science90(8)2850-60(2012 Aug)

Calpain3 is expressed in a proteolitically active form in papillomavirus-associated urothelial tumors of the urinary bladder in cattle.
Roperto S, De Tullio R, Raso C, Stifanese R, Russo V, Gaspari M, Borzacchiello G, Averna M, Paciello O, Cuda G, Roperto F
PloS one5(4)e10299(2010 Apr)

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

Variation at the Calpain 3 gene is associated with meat tenderness in zebu and composite breeds of cattle.
Barendse W, Harrison BE, Bunch RJ, Thomas MB
BMC genetics941(2008 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

2 CAPN3 transcripts in the skeletal muscle individuals of local black cattle from Jilin, China, were identified.
Title: Characterization of a novel CAPN3 transcript generated by alternative splicing in cattle.

Calpain-system gene markers have beneficial effects on eating quality, consistent with previous findings for objective meat quality.
Title: Production and processing studies on calpain-system gene markers for beef tenderness: consumer assessments of eating quality.

activated Capn3 may be involved in molecular pathways leading to the overexpression of E2F3, which in turn could be responsible for urothelial tumor cell proliferation
Title: Calpain3 is expressed in a proteolitically active form in papillomavirus-associated urothelial tumors of the urinary bladder in cattle.

A QTL for meat tenderness is located in the Calpain 3 region of bovine chromosome 10.
Title: Variation at the Calpain 3 gene is associated with meat tenderness in zebu and composite breeds of cattle.

The following CAPN3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CAPN3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa43478	NM_174260.2 Latest version!	Bos taurus calpain 3 (CAPN3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OBa219403	XM_024997263.1 Latest version!	Bos taurus calpain 3 (CAPN3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OBa219404	XM_024997264.1 Latest version!	Bos taurus calpain 3 (CAPN3), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OBa219405	XM_024997265.1 Latest version!	Bos taurus calpain 3 (CAPN3), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OBa91663	XM_015473111.2 Latest version!	Bos taurus calpain 3 (CAPN3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
View Old Accession Versions >>
OBa91663	XM_015473111.1	Bos taurus calpain 3 (CAPN3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CAPN3 ( NM_174260.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_776685.1 Bos taurus calpain 3 (CAPN3), mRNA.

Price & Availability↑

CloneID	OBa43478
Clone ID Related Accession (Same CDS sequence)	NM_174260.2
Accession Version	NM_174260.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2469bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Bos taurus(cattle)
Product	calpain-3
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF087569.1. On Jun 3, 2003 this sequence version replaced NM_174260.1. ##Evidence-Data-START## Transcript exon combination :: AF087569.1, BC149197.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145418, SAMN03145430 [ECO:0000348] ##Evidence-Data-END##

ATGCCGACCG TCATTAGCGC CTCTGTGGCC CCACGGACAG GGGCTGAGCC CATGTCCCCA 
GGGCCCATCG CCCAGGCAGC CCAGGACAAG GGCACCGAGG CAGGGGGTGG AAACCCAAGT 
GGCATCTACT CAGCCATCAT CAGCCGCAAT TTTCCCATTA TTGGGGTGAA AGAGAAGACA 
TTCGAGCAGC TTCACAAGAA ATGTCTGGAA AAGAAGGTTC TTTTTGTGGA TCCTGAGTTC 
CCACCGGACG AGACCTCCCT GTTTTACAGC CAGAAGTTCC CCATCCAGTT CGTCTGGAAG 
AGACCTCCGG AAATTTGTGA GAATCCCCGA TTTATCGTTG GTGGAGCCAA TAGAACTGAC 
ATCTGCCAAG GAGATCTAGG GGACTGCTGG TTTCTTGCAG CCATCGCTTG CCTGACCTTG 
AACAAGCGTC TGCTTTTCCG GGTCATACCC CATGATCAGA GTTTCACCGA AAACTACGCG 
GGGATTTTTC ACTTCCAGTT CTGGCGCTAT GGAGACTGGG TGGACGTGGT TATTGATGAC 
TGCCTGCCAA CCTACAACAA TCAACTGGTT TTCACCAAAT CCAACCATCG CAATGAGTTC 
TGGAATGCTC TGCTGGAGAA GGCTTATGCT AAGCTCCATG GTTCGTATGA AGCCCTGAAA 
GGTGGGAACA CTACAGAGGC CATGGAGGAC TTCACGGGAG GAGTGACAGA GTTTTTTGAA 
ATCAAGGATG CTCCCAGAGA CATGTACAAG ATCATGAAGA AAGCCATCGA GAGGGGTTCC 
CTCATGGGCT GCTCCATTGA TGATGGCACA AACATGACCT ATGGAACCTC TCCTTCTGGG 
CTGAAAATGG GGGAGTTGAT TGAGCGGATG GTGAGGAATA TGGATAACTC GCGGCTCAGG 
GACTCAGACC TCATCCCTGA GGGATGCTCA GATGACAGAC CAACTCGGAT GATTGTTCCA 
GTTCAGTTTG AGACAAGAAT GGCCTGTGGG CTGGTCAAAG GCCATGCCTA CTCAGTCACT 
GGGCTGGAGG AGGCCCTGTA CAAGGGTGAG AAAGTGAAGC TGGTGCGGCT GCGGAACCCC 
TGGGGCCAGG TGGAGTGGAA TGGCTCCTGG AGTGACAGCT GGAAGGACTG GAGCTATGTG 
GACAAGGACG AGAAGGCCCG TTTGCAGCAC CAGGTCACTG AGGATGGAGA GTTCTGGATG 
TCCTACGATG ATTTTATCTA CCATTTCACA AAGCTGGAGA TCTGCAACCT CACAGCTGAT 
GCCCTGGAGT CCGACAAGCT TCAGACTTGG ACAGTGTCCG TGAATGAGGG CCGCTGGGTG 
AGGGGCTGCT CTGCCGGAGG CTGCCGCAAC TTCCCAGACA CTTTCTGGAC CAACCCACAG 
TACCGTCTGA AGCTCCTAGA GGAGGACGAC GACCCCGATG ATTCCGAGGT GATCTGTAGT 
TTCCTGGTGG CTCTGATGCA GAAGAACCGG AGGAAGGACC GGAAGCTGGG GGCTAACCTC 
TTCACCATCG GTTTCGCCAT CTACGAGGTT CCCAAAGAGA TGCACGGCAA CAAGCAGCAC 
CTGCAGAAGG ACTTCTTCCT GTACAATGCC TCCAAGGCTA GGAGCAGAAC CTACATCAAC 
ATGCGGGAGG TGTCTGAGCG CTTCCGCCTG CCTCCCAGCG AGTACGTCAT TGTGCCCTCC 
ACTTACGAGC CCCACCAGGA GGGCGAGTTC ATCCTCCGGG TCTTCTCGGA AAAGAGGAAC 
CTCTCTGAGG AAGTTGAGAA TACAATCTCT GTGGATCGGC CAGTGAAAAA GAAAAAAAAC 
AAGCCCATCA TCTTTGTTTC AGACCGAGCA AACAGCAACA AGGAGCTGGG TGTGGACCAG 
GAAACAGAGG AGGGAAAAGA CAACACAAGC CCTGATAAGC AAGCAAAATC CCCACAGCTA 
GAGCCTGGCA ACACCGACCA GGAAAGTGAG GAACAGCGGC AATTCCGGAA TATTTTCAGG 
CAGATAGCAG GCGATGACAT GGAGATCTGC GCAGATGAGC TCAAGAACGT CCTTAACAGA 
GTTGTGAACA AACATAAGGA CCTGAAGACA CAAGGCTTCA CGCTGGAGTC CTGCCGTAGC 
ATGATTGCTC TCATGGACAC AGATGGCTCT GGAAGACTGA ACCTGCAAGA GTTTCATCAC 
CTCTGGAAGA AGATTAAGAC GTGGCAGAAA ATTTTCAAAC ACTATGACAC AGACCAATCT 
GGCACCATCA ACAGCTACGA GATGCGCAAT GCAGTCAAAG ATGCAGGCTT CCACCTCAAC 
AACCAGCTCT ACGATATCAT TACCATGCGC TATGCAGACA AGTACATGAA TATTGACTTC 
GACAGTTTCA TCTGCTGCTT TGTCAGGCTG GAGGGCATGT TCAGAGCTTT CAATGCATTT 
GACAAGGATG GGGACGGTAT CATCAAACTC AATGTTCTCG AGTGGCTGCA GCTCACCATG 
TATGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_776685.1
CDS	70..2538
Translation	MPTVISASVAPRTGAEPMSPGPIAQAAQDKGTEAGGGNPSGIYSAIISRNFPIIGVKEKTFEQLHKKCLEKKVLFVDPEFPPDETSLFYSQKFPIQFVWKRPPEICENPRFIVGGANRTDICQGDLGDCWFLAAIACLTLNKRLLFRVIPHDQSFTENYAGIFHFQFWRYGDWVDVVIDDCLPTYNNQLVFTKSNHRNEFWNALLEKAYAKLHGSYEALKGGNTTEAMEDFTGGVTEFFEIKDAPRDMYKIMKKAIERGSLMGCSIDDGTNMTYGTSPSGLKMGELIERMVRNMDNSRLRDSDLIPEGCSDDRPTRMIVPVQFETRMACGLVKGHAYSVTGLEEALYKGEKVKLVRLRNPWGQVEWNGSWSDSWKDWSYVDKDEKARLQHQVTEDGEFWMSYDDFIYHFTKLEICNLTADALESDKLQTWTVSVNEGRWVRGCSAGGCRNFPDTFWTNPQYRLKLLEEDDDPDDSEVICSFLVALMQKNRRKDRKLGANLFTIGFAIYEVPKEMHGNKQHLQKDFFLYNASKARSRTYINMREVSERFRLPPSEYVIVPSTYEPHQEGEFILRVFSEKRNLSEEVENTISVDRPVKKKKNKPIIFVSDRANSNKELGVDQETEEGKDNTSPDKQAKSPQLEPGNTDQESEEQRQFRNIFRQIAGDDMEICADELKNVLNRVVNKHKDLKTQGFTLESCRSMIALMDTDGSGRLNLQEFHHLWKKIKTWQKIFKHYDTDQSGTINSYEMRNAVKDAGFHLNNQLYDIITMRYADKYMNIDFDSFICCFVRLEGMFRAFNAFDKDGDGIIKLNVLEWLQLTMYA

Target ORF information:

RefSeq Version	NM_174260.2
Organism	Bos taurus(cattle)
Definition	Bos taurus calpain 3 (CAPN3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_174260.2

ORF Insert Sequence:

ATGCCGACCG TCATTAGCGC CTCTGTGGCC CCACGGACAG GGGCTGAGCC CATGTCCCCA 
GGGCCCATCG CCCAGGCAGC CCAGGACAAG GGCACCGAGG CAGGGGGTGG AAACCCAAGT 
GGCATCTACT CAGCCATCAT CAGCCGCAAT TTTCCCATTA TTGGGGTGAA AGAGAAGACA 
TTCGAGCAGC TTCACAAGAA ATGTCTGGAA AAGAAGGTTC TTTTTGTGGA TCCTGAGTTC 
CCACCGGACG AGACCTCCCT GTTTTACAGC CAGAAGTTCC CCATCCAGTT CGTCTGGAAG 
AGACCTCCGG AAATTTGTGA GAATCCCCGA TTTATCGTTG GTGGAGCCAA TAGAACTGAC 
ATCTGCCAAG GAGATCTAGG GGACTGCTGG TTTCTTGCAG CCATCGCTTG CCTGACCTTG 
AACAAGCGTC TGCTTTTCCG GGTCATACCC CATGATCAGA GTTTCACCGA AAACTACGCG 
GGGATTTTTC ACTTCCAGTT CTGGCGCTAT GGAGACTGGG TGGACGTGGT TATTGATGAC 
TGCCTGCCAA CCTACAACAA TCAACTGGTT TTCACCAAAT CCAACCATCG CAATGAGTTC 
TGGAATGCTC TGCTGGAGAA GGCTTATGCT AAGCTCCATG GTTCGTATGA AGCCCTGAAA 
GGTGGGAACA CTACAGAGGC CATGGAGGAC TTCACGGGAG GAGTGACAGA GTTTTTTGAA 
ATCAAGGATG CTCCCAGAGA CATGTACAAG ATCATGAAGA AAGCCATCGA GAGGGGTTCC 
CTCATGGGCT GCTCCATTGA TGATGGCACA AACATGACCT ATGGAACCTC TCCTTCTGGG 
CTGAAAATGG GGGAGTTGAT TGAGCGGATG GTGAGGAATA TGGATAACTC GCGGCTCAGG 
GACTCAGACC TCATCCCTGA GGGATGCTCA GATGACAGAC CAACTCGGAT GATTGTTCCA 
GTTCAGTTTG AGACAAGAAT GGCCTGTGGG CTGGTCAAAG GCCATGCCTA CTCAGTCACT 
GGGCTGGAGG AGGCCCTGTA CAAGGGTGAG AAAGTGAAGC TGGTGCGGCT GCGGAACCCC 
TGGGGCCAGG TGGAGTGGAA TGGCTCCTGG AGTGACAGCT GGAAGGACTG GAGCTATGTG 
GACAAGGACG AGAAGGCCCG TTTGCAGCAC CAGGTCACTG AGGATGGAGA GTTCTGGATG 
TCCTACGATG ATTTTATCTA CCATTTCACA AAGCTGGAGA TCTGCAACCT CACAGCTGAT 
GCCCTGGAGT CCGACAAGCT TCAGACTTGG ACAGTGTCCG TGAATGAGGG CCGCTGGGTG 
AGGGGCTGCT CTGCCGGAGG CTGCCGCAAC TTCCCAGACA CTTTCTGGAC CAACCCACAG 
TACCGTCTGA AGCTCCTAGA GGAGGACGAC GACCCCGATG ATTCCGAGGT GATCTGTAGT 
TTCCTGGTGG CTCTGATGCA GAAGAACCGG AGGAAGGACC GGAAGCTGGG GGCTAACCTC 
TTCACCATCG GTTTCGCCAT CTACGAGGTT CCCAAAGAGA TGCACGGCAA CAAGCAGCAC 
CTGCAGAAGG ACTTCTTCCT GTACAATGCC TCCAAGGCTA GGAGCAGAAC CTACATCAAC 
ATGCGGGAGG TGTCTGAGCG CTTCCGCCTG CCTCCCAGCG AGTACGTCAT TGTGCCCTCC 
ACTTACGAGC CCCACCAGGA GGGCGAGTTC ATCCTCCGGG TCTTCTCGGA AAAGAGGAAC 
CTCTCTGAGG AAGTTGAGAA TACAATCTCT GTGGATCGGC CAGTGAAAAA GAAAAAAAAC 
AAGCCCATCA TCTTTGTTTC AGACCGAGCA AACAGCAACA AGGAGCTGGG TGTGGACCAG 
GAAACAGAGG AGGGAAAAGA CAACACAAGC CCTGATAAGC AAGCAAAATC CCCACAGCTA 
GAGCCTGGCA ACACCGACCA GGAAAGTGAG GAACAGCGGC AATTCCGGAA TATTTTCAGG 
CAGATAGCAG GCGATGACAT GGAGATCTGC GCAGATGAGC TCAAGAACGT CCTTAACAGA 
GTTGTGAACA AACATAAGGA CCTGAAGACA CAAGGCTTCA CGCTGGAGTC CTGCCGTAGC 
ATGATTGCTC TCATGGACAC AGATGGCTCT GGAAGACTGA ACCTGCAAGA GTTTCATCAC 
CTCTGGAAGA AGATTAAGAC GTGGCAGAAA ATTTTCAAAC ACTATGACAC AGACCAATCT 
GGCACCATCA ACAGCTACGA GATGCGCAAT GCAGTCAAAG ATGCAGGCTT CCACCTCAAC 
AACCAGCTCT ACGATATCAT TACCATGCGC TATGCAGACA AGTACATGAA TATTGACTTC 
GACAGTTTCA TCTGCTGCTT TGTCAGGCTG GAGGGCATGT TCAGAGCTTT CAATGCATTT 
GACAAGGATG GGGACGGTAT CATCAAACTC AATGTTCTCG AGTGGCTGCA GCTCACCATG 
TATGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CAPN3 ( XM_024997263.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024853031.1 Bos taurus calpain 3 (CAPN3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa219403
Clone ID Related Accession (Same CDS sequence)	XM_024997263.1
Accession Version	XM_024997263.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2451bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	calpain-3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGACCG TCATTAGCGC CTCTGTGGCC CCACGGACAG GGGCTGAGCC CATGTCCCCA 
GGGCCCATCG CCCAGGCAGC CCAGGACAAG GGCACCGAGG CAGGGGGTGG AAACCCAAGT 
GGCATCTACT CAGCCATCAT CAGCCGCAAT TTTCCCATTA TTGGGGTGAA AGAGAAGACA 
TTCGAGCAGC TTCACAAGAA ATGTCTGGAA AAGAAGGTTC TTTTTGTGGA TCCTGAGTTC 
CCACCGGACG AGACCTCCCT GTTTTACAGC CAGAAGTTCC CCATCCAGTT CGTCTGGAAG 
AGACCTCCGG AAATTTGTGA GAATCCCCGA TTTATCGTTG GTGGAGCCAA TAGAACTGAC 
ATCTGCCAAG GAGATCTAGG GGACTGCTGG TTTCTTGCAG CCATCGCTTG CCTGACCTTG 
AACAAGCGTC TGCTTTTCCG GGTCATACCC CATGATCAGA GTTTCACCGA AAACTACGCG 
GGGATTTTTC ACTTCCAGTT CTGGCGCTAT GGAGACTGGG TGGACGTGGT TATTGATGAC 
TGCCTGCCAA CCTACAACAA TCAACTGGTT TTCACCAAAT CCAACCATCG CAATGAGTTC 
TGGAGTGCTC TGCTGGAGAA GGCTTATGCT AAGCTCCATG GTTCGTATGA AGCCCTGAAA 
GGTGGGAACA CTACAGAGGC CATGGAGGAC TTCACGGGAG GAGTGACAGA GTTTTTTGAA 
ATCAAGGATG CTCCCAGAGA CATGTACAAG ATCATGAAGA AAGCCATCGA GAGGGGTTCC 
CTCATGGGCT GCTCCATTGA TGATGGCACA AACATGACCT ATGGAACCTC TCCTTCTGGG 
CTGAAAATGG GGGAGTTGAT TGAGCGGATG GTGAGGAATA TGGATAACTC GCGGCTCAGG 
GACTCAGACC TCATCCCTGA GGGATGCTCA GATGACAGAC CAACTCGGAT GATTGTTCCA 
GTTCAGTTTG AGACAAGAAT GGCCTGTGGG CTGGTCAAAG GCCATGCCTA CTCAGTCACT 
GGGCTGGAGG AGGCCCTGTA CAAGGGTGAG AAAGTGAAGC TGGTGCGGCT GCGGAACCCC 
TGGGGCCAGG TGGAGTGGAA TGGCTCCTGG AGTGACAGCT GGAAGGACTG GAGCTATGTG 
GACAAGGACG AGAAGGCCCG TTTGCAGCAC CAGGTCACTG AGGATGGAGA GTTCTGGATG 
TCCTACGATG ATTTTATCTA CCATTTCACA AAGCTGGAGA TCTGCAACCT CACAGCTGAT 
GCCCTGGAGT CCGACAAGCT TCAGACTTGG ACAGTGTCCG TGAATGAGGG CCGCTGGGTG 
AGGGGCTGCT CTGCCGGAGG CTGCCGCAAC TTCCCAGACA CTTTCTGGAC CAACCCACAG 
TACCGTCTGA AGCTCCTAGA GGAGGACGAC GACCCCGATG ATTCCGAGGT GATCTGTAGT 
TTCCTGGTGG CTCTGATGCA GAAGAACCGG AGGAAGGACC GGAAGCTGGG GGCTAACCTC 
TTCACCATCG GTTTCGCCAT CTACGAGGTT CCCAAAGAGA TGCACGGCAA CAAGCAGCAC 
CTGCAGAAGG ACTTCTTCCT GTACAATGCC TCCAAGGCTA GGAGCAGAAC CTACATCAAC 
ATGCGGGAGG TGTCTGAGCG CTTCCGCCTG CCTCCCAGCG AGTACGTCAT TGTGCCCTCC 
ACTTACGAGC CCCACCAGGA GGGCGAGTTC ATCCTCCGGG TCTTCTCGGA AAAGAGGAAC 
CTCTCTGAGG AAGTTGAGAA TACAATCTCT GTGGATCGGC CAGTGCCCAT CATCTTTGTT 
TCAGACCGAG CAAACAGCAA CAAGGAGCTG GGTGTGGACC AGGAAACAGA GGAGGGAAAA 
GACAACACAA GCCCTGATAA GCAAGCAAAA TCCCCACAGC TAGAGCCTGG CAACACCGAC 
CAGGAAAGTG AGGAACAGCG GCAATTCCGG AATATTTTCA GGCAGATAGC AGGCGATGAC 
ATGGAGATCT GCGCAGATGA GCTCAAGAAC GTCCTTAACA GAGTTGTGAA CAAACATAAG 
GACCTGAAGA CACAAGGCTT CACGCTGGAG TCCTGCCGTA GCATGATTGC TCTCATGGAC 
ACAGATGGCT CTGGGAGACT GAACCTGCAA GAGTTTCATC ACCTCTGGAA GAAGATTAAG 
ACGTGGCAGA AAATTTTCAA ACACTATGAC ACAGACCAAT CTGGCACCAT CAACAGCTAC 
GAGATGCGCA ATGCAGTCAA AGATGCAGGC TTCCACCTCA ACAACCAGCT CTACGATATC 
ATTACCATGC GCTATGCAGA CAAGTACATG AATATTGACT TCGACAGTTT CATCTGCTGC 
TTTGTCAGGC TGGAGGGCAT GTTCAGAGCT TTCAATGCAT TTGACAAGGA TGGGGACGGT 
ATCATCAAAC TCAATGTTCT CGAGTGGCTG CAGCTCACCA TGTATGCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024853031.1
CDS	304..2754
Translation	MPTVISASVAPRTGAEPMSPGPIAQAAQDKGTEAGGGNPSGIYSAIISRNFPIIGVKEKTFEQLHKKCLEKKVLFVDPEFPPDETSLFYSQKFPIQFVWKRPPEICENPRFIVGGANRTDICQGDLGDCWFLAAIACLTLNKRLLFRVIPHDQSFTENYAGIFHFQFWRYGDWVDVVIDDCLPTYNNQLVFTKSNHRNEFWSALLEKAYAKLHGSYEALKGGNTTEAMEDFTGGVTEFFEIKDAPRDMYKIMKKAIERGSLMGCSIDDGTNMTYGTSPSGLKMGELIERMVRNMDNSRLRDSDLIPEGCSDDRPTRMIVPVQFETRMACGLVKGHAYSVTGLEEALYKGEKVKLVRLRNPWGQVEWNGSWSDSWKDWSYVDKDEKARLQHQVTEDGEFWMSYDDFIYHFTKLEICNLTADALESDKLQTWTVSVNEGRWVRGCSAGGCRNFPDTFWTNPQYRLKLLEEDDDPDDSEVICSFLVALMQKNRRKDRKLGANLFTIGFAIYEVPKEMHGNKQHLQKDFFLYNASKARSRTYINMREVSERFRLPPSEYVIVPSTYEPHQEGEFILRVFSEKRNLSEEVENTISVDRPVPIIFVSDRANSNKELGVDQETEEGKDNTSPDKQAKSPQLEPGNTDQESEEQRQFRNIFRQIAGDDMEICADELKNVLNRVVNKHKDLKTQGFTLESCRSMIALMDTDGSGRLNLQEFHHLWKKIKTWQKIFKHYDTDQSGTINSYEMRNAVKDAGFHLNNQLYDIITMRYADKYMNIDFDSFICCFVRLEGMFRAFNAFDKDGDGIIKLNVLEWLQLTMYA

Target ORF information:

RefSeq Version	XM_024997263.1
Organism	Bos taurus(cattle)
Definition	Bos taurus calpain 3 (CAPN3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024997263.1

ORF Insert Sequence:

ATGCCGACCG TCATTAGCGC CTCTGTGGCC CCACGGACAG GGGCTGAGCC CATGTCCCCA 
GGGCCCATCG CCCAGGCAGC CCAGGACAAG GGCACCGAGG CAGGGGGTGG AAACCCAAGT 
GGCATCTACT CAGCCATCAT CAGCCGCAAT TTTCCCATTA TTGGGGTGAA AGAGAAGACA 
TTCGAGCAGC TTCACAAGAA ATGTCTGGAA AAGAAGGTTC TTTTTGTGGA TCCTGAGTTC 
CCACCGGACG AGACCTCCCT GTTTTACAGC CAGAAGTTCC CCATCCAGTT CGTCTGGAAG 
AGACCTCCGG AAATTTGTGA GAATCCCCGA TTTATCGTTG GTGGAGCCAA TAGAACTGAC 
ATCTGCCAAG GAGATCTAGG GGACTGCTGG TTTCTTGCAG CCATCGCTTG CCTGACCTTG 
AACAAGCGTC TGCTTTTCCG GGTCATACCC CATGATCAGA GTTTCACCGA AAACTACGCG 
GGGATTTTTC ACTTCCAGTT CTGGCGCTAT GGAGACTGGG TGGACGTGGT TATTGATGAC 
TGCCTGCCAA CCTACAACAA TCAACTGGTT TTCACCAAAT CCAACCATCG CAATGAGTTC 
TGGAGTGCTC TGCTGGAGAA GGCTTATGCT AAGCTCCATG GTTCGTATGA AGCCCTGAAA 
GGTGGGAACA CTACAGAGGC CATGGAGGAC TTCACGGGAG GAGTGACAGA GTTTTTTGAA 
ATCAAGGATG CTCCCAGAGA CATGTACAAG ATCATGAAGA AAGCCATCGA GAGGGGTTCC 
CTCATGGGCT GCTCCATTGA TGATGGCACA AACATGACCT ATGGAACCTC TCCTTCTGGG 
CTGAAAATGG GGGAGTTGAT TGAGCGGATG GTGAGGAATA TGGATAACTC GCGGCTCAGG 
GACTCAGACC TCATCCCTGA GGGATGCTCA GATGACAGAC CAACTCGGAT GATTGTTCCA 
GTTCAGTTTG AGACAAGAAT GGCCTGTGGG CTGGTCAAAG GCCATGCCTA CTCAGTCACT 
GGGCTGGAGG AGGCCCTGTA CAAGGGTGAG AAAGTGAAGC TGGTGCGGCT GCGGAACCCC 
TGGGGCCAGG TGGAGTGGAA TGGCTCCTGG AGTGACAGCT GGAAGGACTG GAGCTATGTG 
GACAAGGACG AGAAGGCCCG TTTGCAGCAC CAGGTCACTG AGGATGGAGA GTTCTGGATG 
TCCTACGATG ATTTTATCTA CCATTTCACA AAGCTGGAGA TCTGCAACCT CACAGCTGAT 
GCCCTGGAGT CCGACAAGCT TCAGACTTGG ACAGTGTCCG TGAATGAGGG CCGCTGGGTG 
AGGGGCTGCT CTGCCGGAGG CTGCCGCAAC TTCCCAGACA CTTTCTGGAC CAACCCACAG 
TACCGTCTGA AGCTCCTAGA GGAGGACGAC GACCCCGATG ATTCCGAGGT GATCTGTAGT 
TTCCTGGTGG CTCTGATGCA GAAGAACCGG AGGAAGGACC GGAAGCTGGG GGCTAACCTC 
TTCACCATCG GTTTCGCCAT CTACGAGGTT CCCAAAGAGA TGCACGGCAA CAAGCAGCAC 
CTGCAGAAGG ACTTCTTCCT GTACAATGCC TCCAAGGCTA GGAGCAGAAC CTACATCAAC 
ATGCGGGAGG TGTCTGAGCG CTTCCGCCTG CCTCCCAGCG AGTACGTCAT TGTGCCCTCC 
ACTTACGAGC CCCACCAGGA GGGCGAGTTC ATCCTCCGGG TCTTCTCGGA AAAGAGGAAC 
CTCTCTGAGG AAGTTGAGAA TACAATCTCT GTGGATCGGC CAGTGCCCAT CATCTTTGTT 
TCAGACCGAG CAAACAGCAA CAAGGAGCTG GGTGTGGACC AGGAAACAGA GGAGGGAAAA 
GACAACACAA GCCCTGATAA GCAAGCAAAA TCCCCACAGC TAGAGCCTGG CAACACCGAC 
CAGGAAAGTG AGGAACAGCG GCAATTCCGG AATATTTTCA GGCAGATAGC AGGCGATGAC 
ATGGAGATCT GCGCAGATGA GCTCAAGAAC GTCCTTAACA GAGTTGTGAA CAAACATAAG 
GACCTGAAGA CACAAGGCTT CACGCTGGAG TCCTGCCGTA GCATGATTGC TCTCATGGAC 
ACAGATGGCT CTGGGAGACT GAACCTGCAA GAGTTTCATC ACCTCTGGAA GAAGATTAAG 
ACGTGGCAGA AAATTTTCAA ACACTATGAC ACAGACCAAT CTGGCACCAT CAACAGCTAC 
GAGATGCGCA ATGCAGTCAA AGATGCAGGC TTCCACCTCA ACAACCAGCT CTACGATATC 
ATTACCATGC GCTATGCAGA CAAGTACATG AATATTGACT TCGACAGTTT CATCTGCTGC 
TTTGTCAGGC TGGAGGGCAT GTTCAGAGCT TTCAATGCAT TTGACAAGGA TGGGGACGGT 
ATCATCAAAC TCAATGTTCT CGAGTGGCTG CAGCTCACCA TGTATGCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CAPN3 ( XM_024997264.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024853032.1 Bos taurus calpain 3 (CAPN3), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa219404
Clone ID Related Accession (Same CDS sequence)	XM_024997264.1
Accession Version	XM_024997264.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2322bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	calpain-3 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGACCG TCATTAGCGC CTCTGTGGCC CCACGGACAG GGGCTGAGCC CATGTCCCCA 
GGGCCCATCG CCCAGGCAGC CCAGGACAAG GGCACCGAGG CAGGGGGTGG AAACCCAAGT 
GGCATCTACT CAGCCATCAT CAGCCGCAAT TTTCCCATTA TTGGGGTGAA AGAGAAGACA 
TTCGAGCAGC TTCACAAGAA ATGTCTGGAA AAGAAGGTTC TTTTTGTGGA TCCTGAGTTC 
CCACCGGACG AGACCTCCCT GTTTTACAGC CAGAAGTTCC CCATCCAGTT CGTCTGGAAG 
AGACCTCCGG AAATTTGTGA GAATCCCCGA TTTATCGTTG GTGGAGCCAA TAGAACTGAC 
ATCTGCCAAG GAGATCTAGG GGACTGCTGG TTTCTTGCAG CCATCGCTTG CCTGACCTTG 
AACAAGCGTC TGCTTTTCCG GGTCATACCC CATGATCAGA GTTTCACCGA AAACTACGCG 
GGGATTTTTC ACTTCCAGTT CTGGCGCTAT GGAGACTGGG TGGACGTGGT TATTGATGAC 
TGCCTGCCAA CCTACAACAA TCAACTGGTT TTCACCAAAT CCAACCATCG CAATGAGTTC 
TGGAGTGCTC TGCTGGAGAA GGCTTATGCT AAGCTCCATG GTTCGTATGA AGCCCTGAAA 
GGTGGGAACA CTACAGAGGC CATGGAGGAC TTCACGGGAG GAGTGACAGA GTTTTTTGAA 
ATCAAGGATG CTCCCAGAGA CATGTACAAG ATCATGAAGA AAGCCATCGA GAGGGGTTCC 
CTCATGGGCT GCTCCATTGA TATGATTGTT CCAGTTCAGT TTGAGACAAG AATGGCCTGT 
GGGCTGGTCA AAGGCCATGC CTACTCAGTC ACTGGGCTGG AGGAGGCCCT GTACAAGGGT 
GAGAAAGTGA AGCTGGTGCG GCTGCGGAAC CCCTGGGGCC AGGTGGAGTG GAATGGCTCC 
TGGAGTGACA GCTGGAAGGA CTGGAGCTAT GTGGACAAGG ACGAGAAGGC CCGTTTGCAG 
CACCAGGTCA CTGAGGATGG AGAGTTCTGG ATGTCCTACG ATGATTTTAT CTACCATTTC 
ACAAAGCTGG AGATCTGCAA CCTCACAGCT GATGCCCTGG AGTCCGACAA GCTTCAGACT 
TGGACAGTGT CCGTGAATGA GGGCCGCTGG GTGAGGGGCT GCTCTGCCGG AGGCTGCCGC 
AACTTCCCAG ACACTTTCTG GACCAACCCA CAGTACCGTC TGAAGCTCCT AGAGGAGGAC 
GACGACCCCG ATGATTCCGA GGTGATCTGT AGTTTCCTGG TGGCTCTGAT GCAGAAGAAC 
CGGAGGAAGG ACCGGAAGCT GGGGGCTAAC CTCTTCACCA TCGGTTTCGC CATCTACGAG 
GTTCCCAAAG AGATGCACGG CAACAAGCAG CACCTGCAGA AGGACTTCTT CCTGTACAAT 
GCCTCCAAGG CTAGGAGCAG AACCTACATC AACATGCGGG AGGTGTCTGA GCGCTTCCGC 
CTGCCTCCCA GCGAGTACGT CATTGTGCCC TCCACTTACG AGCCCCACCA GGAGGGCGAG 
TTCATCCTCC GGGTCTTCTC GGAAAAGAGG AACCTCTCTG AGGAAGTTGA GAATACAATC 
TCTGTGGATC GGCCAGTGAA AAAGAAAAAA AACAAGCCCA TCATCTTTGT TTCAGACCGA 
GCAAACAGCA ACAAGGAGCT GGGTGTGGAC CAGGAAACAG AGGAGGGAAA AGACAACACA 
AGCCCTGATA AGCAAGCAAA ATCCCCACAG CTAGAGCCTG GCAACACCGA CCAGGAAAGT 
GAGGAACAGC GGCAATTCCG GAATATTTTC AGGCAGATAG CAGGCGATGA CATGGAGATC 
TGCGCAGATG AGCTCAAGAA CGTCCTTAAC AGAGTTGTGA ACAAACATAA GGACCTGAAG 
ACACAAGGCT TCACGCTGGA GTCCTGCCGT AGCATGATTG CTCTCATGGA CACAGATGGC 
TCTGGGAGAC TGAACCTGCA AGAGTTTCAT CACCTCTGGA AGAAGATTAA GACGTGGCAG 
AAAATTTTCA AACACTATGA CACAGACCAA TCTGGCACCA TCAACAGCTA CGAGATGCGC 
AATGCAGTCA AAGATGCAGG CTTCCACCTC AACAACCAGC TCTACGATAT CATTACCATG 
CGCTATGCAG ACAAGTACAT GAATATTGAC TTCGACAGTT TCATCTGCTG CTTTGTCAGG 
CTGGAGGGCA TGTTCAGAGC TTTCAATGCA TTTGACAAGG ATGGGGACGG TATCATCAAA 
CTCAATGTTC TCGAGTGGCT GCAGCTCACC ATGTATGCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024853032.1
CDS	304..2625
Translation	MPTVISASVAPRTGAEPMSPGPIAQAAQDKGTEAGGGNPSGIYSAIISRNFPIIGVKEKTFEQLHKKCLEKKVLFVDPEFPPDETSLFYSQKFPIQFVWKRPPEICENPRFIVGGANRTDICQGDLGDCWFLAAIACLTLNKRLLFRVIPHDQSFTENYAGIFHFQFWRYGDWVDVVIDDCLPTYNNQLVFTKSNHRNEFWSALLEKAYAKLHGSYEALKGGNTTEAMEDFTGGVTEFFEIKDAPRDMYKIMKKAIERGSLMGCSIDMIVPVQFETRMACGLVKGHAYSVTGLEEALYKGEKVKLVRLRNPWGQVEWNGSWSDSWKDWSYVDKDEKARLQHQVTEDGEFWMSYDDFIYHFTKLEICNLTADALESDKLQTWTVSVNEGRWVRGCSAGGCRNFPDTFWTNPQYRLKLLEEDDDPDDSEVICSFLVALMQKNRRKDRKLGANLFTIGFAIYEVPKEMHGNKQHLQKDFFLYNASKARSRTYINMREVSERFRLPPSEYVIVPSTYEPHQEGEFILRVFSEKRNLSEEVENTISVDRPVKKKKNKPIIFVSDRANSNKELGVDQETEEGKDNTSPDKQAKSPQLEPGNTDQESEEQRQFRNIFRQIAGDDMEICADELKNVLNRVVNKHKDLKTQGFTLESCRSMIALMDTDGSGRLNLQEFHHLWKKIKTWQKIFKHYDTDQSGTINSYEMRNAVKDAGFHLNNQLYDIITMRYADKYMNIDFDSFICCFVRLEGMFRAFNAFDKDGDGIIKLNVLEWLQLTMYA

Target ORF information:

RefSeq Version	XM_024997264.1
Organism	Bos taurus(cattle)
Definition	Bos taurus calpain 3 (CAPN3), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024997264.1

ORF Insert Sequence:

ATGCCGACCG TCATTAGCGC CTCTGTGGCC CCACGGACAG GGGCTGAGCC CATGTCCCCA 
GGGCCCATCG CCCAGGCAGC CCAGGACAAG GGCACCGAGG CAGGGGGTGG AAACCCAAGT 
GGCATCTACT CAGCCATCAT CAGCCGCAAT TTTCCCATTA TTGGGGTGAA AGAGAAGACA 
TTCGAGCAGC TTCACAAGAA ATGTCTGGAA AAGAAGGTTC TTTTTGTGGA TCCTGAGTTC 
CCACCGGACG AGACCTCCCT GTTTTACAGC CAGAAGTTCC CCATCCAGTT CGTCTGGAAG 
AGACCTCCGG AAATTTGTGA GAATCCCCGA TTTATCGTTG GTGGAGCCAA TAGAACTGAC 
ATCTGCCAAG GAGATCTAGG GGACTGCTGG TTTCTTGCAG CCATCGCTTG CCTGACCTTG 
AACAAGCGTC TGCTTTTCCG GGTCATACCC CATGATCAGA GTTTCACCGA AAACTACGCG 
GGGATTTTTC ACTTCCAGTT CTGGCGCTAT GGAGACTGGG TGGACGTGGT TATTGATGAC 
TGCCTGCCAA CCTACAACAA TCAACTGGTT TTCACCAAAT CCAACCATCG CAATGAGTTC 
TGGAGTGCTC TGCTGGAGAA GGCTTATGCT AAGCTCCATG GTTCGTATGA AGCCCTGAAA 
GGTGGGAACA CTACAGAGGC CATGGAGGAC TTCACGGGAG GAGTGACAGA GTTTTTTGAA 
ATCAAGGATG CTCCCAGAGA CATGTACAAG ATCATGAAGA AAGCCATCGA GAGGGGTTCC 
CTCATGGGCT GCTCCATTGA TATGATTGTT CCAGTTCAGT TTGAGACAAG AATGGCCTGT 
GGGCTGGTCA AAGGCCATGC CTACTCAGTC ACTGGGCTGG AGGAGGCCCT GTACAAGGGT 
GAGAAAGTGA AGCTGGTGCG GCTGCGGAAC CCCTGGGGCC AGGTGGAGTG GAATGGCTCC 
TGGAGTGACA GCTGGAAGGA CTGGAGCTAT GTGGACAAGG ACGAGAAGGC CCGTTTGCAG 
CACCAGGTCA CTGAGGATGG AGAGTTCTGG ATGTCCTACG ATGATTTTAT CTACCATTTC 
ACAAAGCTGG AGATCTGCAA CCTCACAGCT GATGCCCTGG AGTCCGACAA GCTTCAGACT 
TGGACAGTGT CCGTGAATGA GGGCCGCTGG GTGAGGGGCT GCTCTGCCGG AGGCTGCCGC 
AACTTCCCAG ACACTTTCTG GACCAACCCA CAGTACCGTC TGAAGCTCCT AGAGGAGGAC 
GACGACCCCG ATGATTCCGA GGTGATCTGT AGTTTCCTGG TGGCTCTGAT GCAGAAGAAC 
CGGAGGAAGG ACCGGAAGCT GGGGGCTAAC CTCTTCACCA TCGGTTTCGC CATCTACGAG 
GTTCCCAAAG AGATGCACGG CAACAAGCAG CACCTGCAGA AGGACTTCTT CCTGTACAAT 
GCCTCCAAGG CTAGGAGCAG AACCTACATC AACATGCGGG AGGTGTCTGA GCGCTTCCGC 
CTGCCTCCCA GCGAGTACGT CATTGTGCCC TCCACTTACG AGCCCCACCA GGAGGGCGAG 
TTCATCCTCC GGGTCTTCTC GGAAAAGAGG AACCTCTCTG AGGAAGTTGA GAATACAATC 
TCTGTGGATC GGCCAGTGAA AAAGAAAAAA AACAAGCCCA TCATCTTTGT TTCAGACCGA 
GCAAACAGCA ACAAGGAGCT GGGTGTGGAC CAGGAAACAG AGGAGGGAAA AGACAACACA 
AGCCCTGATA AGCAAGCAAA ATCCCCACAG CTAGAGCCTG GCAACACCGA CCAGGAAAGT 
GAGGAACAGC GGCAATTCCG GAATATTTTC AGGCAGATAG CAGGCGATGA CATGGAGATC 
TGCGCAGATG AGCTCAAGAA CGTCCTTAAC AGAGTTGTGA ACAAACATAA GGACCTGAAG 
ACACAAGGCT TCACGCTGGA GTCCTGCCGT AGCATGATTG CTCTCATGGA CACAGATGGC 
TCTGGGAGAC TGAACCTGCA AGAGTTTCAT CACCTCTGGA AGAAGATTAA GACGTGGCAG 
AAAATTTTCA AACACTATGA CACAGACCAA TCTGGCACCA TCAACAGCTA CGAGATGCGC 
AATGCAGTCA AAGATGCAGG CTTCCACCTC AACAACCAGC TCTACGATAT CATTACCATG 
CGCTATGCAG ACAAGTACAT GAATATTGAC TTCGACAGTT TCATCTGCTG CTTTGTCAGG 
CTGGAGGGCA TGTTCAGAGC TTTCAATGCA TTTGACAAGG ATGGGGACGG TATCATCAAA 
CTCAATGTTC TCGAGTGGCT GCAGCTCACC ATGTATGCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CAPN3 ( XM_024997265.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024853033.1 Bos taurus calpain 3 (CAPN3), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBa219405
Clone ID Related Accession (Same CDS sequence)	XM_024997265.1
Accession Version	XM_024997265.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2190bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	calpain-3 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGACCG TCATTAGCGC CTCTGTGGCC CCACGGACAG GGGCTGAGCC CATGTCCCCA 
GGGCCCATCG CCCAGGCAGC CCAGGACAAG GGCACCGAGG CAGGGGGTGG AAACCCAAGT 
GGCATCTACT CAGCCATCAT CAGCCGCAAT TTTCCCATTA TTGGGGTGAA AGAGAAGACA 
TTCGAGCAGC TTCACAAGAA ATGTCTGGAA AAGAAGGTTC TTTTTGTGGA TCCTGAGTTC 
CCACCGGACG AGACCTCCCT GTTTTACAGC CAGAAGTTCC CCATCCAGTT CGTCTGGAAG 
AGACCTCCGG AAATTTGTGA GAATCCCCGA TTTATCGTTG GTGGAGCCAA TAGAACTGAC 
ATCTGCCAAG GAGATCTAGG GGACTGCTGG TTTCTTGCAG CCATCGCTTG CCTGACCTTG 
AACAAGCGTC TGCTTTTCCG GGTCATACCC CATGATCAGA GTTTCACCGA AAACTACGCG 
GGGATTTTTC ACTTCCAGTT CTGGCGCTAT GGAGACTGGG TGGACGTGGT TATTGATGAC 
TGCCTGCCAA CCTACAACAA TCAACTGGTT TTCACCAAAT CCAACCATCG CAATGAGTTC 
TGGAGTGCTC TGCTGGAGAA GGCTTATGCT AAGCTCCATG GTTCGTATGA AGCCCTGAAA 
GGTGGGAACA CTACAGAGGC CATGGAGGAC TTCACGGGAG GAGTGACAGA GTTTTTTGAA 
ATCAAGGATG CTCCCAGAGA CATGTACAAG ATCATGAAGA AAGCCATCGA GAGGGGTTCC 
CTCATGGGCT GCTCCATTGA TATGATTGTT CCAGTTCAGT TTGAGACAAG AATGGCCTGT 
GGGCTGGTCA AAGGCCATGC CTACTCAGTC ACTGGGCTGG AGGAGGCCCT GTACAAGGGT 
GAGAAAGTGA AGCTGGTGCG GCTGCGGAAC CCCTGGGGCC AGGTGGAGTG GAATGGCTCC 
TGGAGTGACA GCTGGAAGGA CTGGAGCTAT GTGGACAAGG ACGAGAAGGC CCGTTTGCAG 
CACCAGGTCA CTGAGGATGG AGAGTTCTGG ATGTCCTACG ATGATTTTAT CTACCATTTC 
ACAAAGCTGG AGATCTGCAA CCTCACAGCT GATGCCCTGG AGTCCGACAA GCTTCAGACT 
TGGACAGTGT CCGTGAATGA GGGCCGCTGG GTGAGGGGCT GCTCTGCCGG AGGCTGCCGC 
AACTTCCCAG ACACTTTCTG GACCAACCCA CAGTACCGTC TGAAGCTCCT AGAGGAGGAC 
GACGACCCCG ATGATTCCGA GGTGATCTGT AGTTTCCTGG TGGCTCTGAT GCAGAAGAAC 
CGGAGGAAGG ACCGGAAGCT GGGGGCTAAC CTCTTCACCA TCGGTTTCGC CATCTACGAG 
GTTCCCAAAG AGATGCACGG CAACAAGCAG CACCTGCAGA AGGACTTCTT CCTGTACAAT 
GCCTCCAAGG CTAGGAGCAG AACCTACATC AACATGCGGG AGGTGTCTGA GCGCTTCCGC 
CTGCCTCCCA GCGAGTACGT CATTGTGCCC TCCACTTACG AGCCCCACCA GGAGGGCGAG 
TTCATCCTCC GGGTCTTCTC GGAAAAGAGG AACCTCTCTG AGGAAGTTGA GAATACAATC 
TCTGTGGATC GGCCAGTGCT AGAGCCTGGC AACACCGACC AGGAAAGTGA GGAACAGCGG 
CAATTCCGGA ATATTTTCAG GCAGATAGCA GGCGATGACA TGGAGATCTG CGCAGATGAG 
CTCAAGAACG TCCTTAACAG AGTTGTGAAC AAACATAAGG ACCTGAAGAC ACAAGGCTTC 
ACGCTGGAGT CCTGCCGTAG CATGATTGCT CTCATGGACA CAGATGGCTC TGGGAGACTG 
AACCTGCAAG AGTTTCATCA CCTCTGGAAG AAGATTAAGA CGTGGCAGAA AATTTTCAAA 
CACTATGACA CAGACCAATC TGGCACCATC AACAGCTACG AGATGCGCAA TGCAGTCAAA 
GATGCAGGCT TCCACCTCAA CAACCAGCTC TACGATATCA TTACCATGCG CTATGCAGAC 
AAGTACATGA ATATTGACTT CGACAGTTTC ATCTGCTGCT TTGTCAGGCT GGAGGGCATG 
TTCAGAGCTT TCAATGCATT TGACAAGGAT GGGGACGGTA TCATCAAACT CAATGTTCTC 
GAGTGGCTGC AGCTCACCAT GTATGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024853033.1
CDS	304..2493
Translation	MPTVISASVAPRTGAEPMSPGPIAQAAQDKGTEAGGGNPSGIYSAIISRNFPIIGVKEKTFEQLHKKCLEKKVLFVDPEFPPDETSLFYSQKFPIQFVWKRPPEICENPRFIVGGANRTDICQGDLGDCWFLAAIACLTLNKRLLFRVIPHDQSFTENYAGIFHFQFWRYGDWVDVVIDDCLPTYNNQLVFTKSNHRNEFWSALLEKAYAKLHGSYEALKGGNTTEAMEDFTGGVTEFFEIKDAPRDMYKIMKKAIERGSLMGCSIDMIVPVQFETRMACGLVKGHAYSVTGLEEALYKGEKVKLVRLRNPWGQVEWNGSWSDSWKDWSYVDKDEKARLQHQVTEDGEFWMSYDDFIYHFTKLEICNLTADALESDKLQTWTVSVNEGRWVRGCSAGGCRNFPDTFWTNPQYRLKLLEEDDDPDDSEVICSFLVALMQKNRRKDRKLGANLFTIGFAIYEVPKEMHGNKQHLQKDFFLYNASKARSRTYINMREVSERFRLPPSEYVIVPSTYEPHQEGEFILRVFSEKRNLSEEVENTISVDRPVLEPGNTDQESEEQRQFRNIFRQIAGDDMEICADELKNVLNRVVNKHKDLKTQGFTLESCRSMIALMDTDGSGRLNLQEFHHLWKKIKTWQKIFKHYDTDQSGTINSYEMRNAVKDAGFHLNNQLYDIITMRYADKYMNIDFDSFICCFVRLEGMFRAFNAFDKDGDGIIKLNVLEWLQLTMYA

Target ORF information:

RefSeq Version	XM_024997265.1
Organism	Bos taurus(cattle)
Definition	Bos taurus calpain 3 (CAPN3), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024997265.1

ORF Insert Sequence:

ATGCCGACCG TCATTAGCGC CTCTGTGGCC CCACGGACAG GGGCTGAGCC CATGTCCCCA 
GGGCCCATCG CCCAGGCAGC CCAGGACAAG GGCACCGAGG CAGGGGGTGG AAACCCAAGT 
GGCATCTACT CAGCCATCAT CAGCCGCAAT TTTCCCATTA TTGGGGTGAA AGAGAAGACA 
TTCGAGCAGC TTCACAAGAA ATGTCTGGAA AAGAAGGTTC TTTTTGTGGA TCCTGAGTTC 
CCACCGGACG AGACCTCCCT GTTTTACAGC CAGAAGTTCC CCATCCAGTT CGTCTGGAAG 
AGACCTCCGG AAATTTGTGA GAATCCCCGA TTTATCGTTG GTGGAGCCAA TAGAACTGAC 
ATCTGCCAAG GAGATCTAGG GGACTGCTGG TTTCTTGCAG CCATCGCTTG CCTGACCTTG 
AACAAGCGTC TGCTTTTCCG GGTCATACCC CATGATCAGA GTTTCACCGA AAACTACGCG 
GGGATTTTTC ACTTCCAGTT CTGGCGCTAT GGAGACTGGG TGGACGTGGT TATTGATGAC 
TGCCTGCCAA CCTACAACAA TCAACTGGTT TTCACCAAAT CCAACCATCG CAATGAGTTC 
TGGAGTGCTC TGCTGGAGAA GGCTTATGCT AAGCTCCATG GTTCGTATGA AGCCCTGAAA 
GGTGGGAACA CTACAGAGGC CATGGAGGAC TTCACGGGAG GAGTGACAGA GTTTTTTGAA 
ATCAAGGATG CTCCCAGAGA CATGTACAAG ATCATGAAGA AAGCCATCGA GAGGGGTTCC 
CTCATGGGCT GCTCCATTGA TATGATTGTT CCAGTTCAGT TTGAGACAAG AATGGCCTGT 
GGGCTGGTCA AAGGCCATGC CTACTCAGTC ACTGGGCTGG AGGAGGCCCT GTACAAGGGT 
GAGAAAGTGA AGCTGGTGCG GCTGCGGAAC CCCTGGGGCC AGGTGGAGTG GAATGGCTCC 
TGGAGTGACA GCTGGAAGGA CTGGAGCTAT GTGGACAAGG ACGAGAAGGC CCGTTTGCAG 
CACCAGGTCA CTGAGGATGG AGAGTTCTGG ATGTCCTACG ATGATTTTAT CTACCATTTC 
ACAAAGCTGG AGATCTGCAA CCTCACAGCT GATGCCCTGG AGTCCGACAA GCTTCAGACT 
TGGACAGTGT CCGTGAATGA GGGCCGCTGG GTGAGGGGCT GCTCTGCCGG AGGCTGCCGC 
AACTTCCCAG ACACTTTCTG GACCAACCCA CAGTACCGTC TGAAGCTCCT AGAGGAGGAC 
GACGACCCCG ATGATTCCGA GGTGATCTGT AGTTTCCTGG TGGCTCTGAT GCAGAAGAAC 
CGGAGGAAGG ACCGGAAGCT GGGGGCTAAC CTCTTCACCA TCGGTTTCGC CATCTACGAG 
GTTCCCAAAG AGATGCACGG CAACAAGCAG CACCTGCAGA AGGACTTCTT CCTGTACAAT 
GCCTCCAAGG CTAGGAGCAG AACCTACATC AACATGCGGG AGGTGTCTGA GCGCTTCCGC 
CTGCCTCCCA GCGAGTACGT CATTGTGCCC TCCACTTACG AGCCCCACCA GGAGGGCGAG 
TTCATCCTCC GGGTCTTCTC GGAAAAGAGG AACCTCTCTG AGGAAGTTGA GAATACAATC 
TCTGTGGATC GGCCAGTGCT AGAGCCTGGC AACACCGACC AGGAAAGTGA GGAACAGCGG 
CAATTCCGGA ATATTTTCAG GCAGATAGCA GGCGATGACA TGGAGATCTG CGCAGATGAG 
CTCAAGAACG TCCTTAACAG AGTTGTGAAC AAACATAAGG ACCTGAAGAC ACAAGGCTTC 
ACGCTGGAGT CCTGCCGTAG CATGATTGCT CTCATGGACA CAGATGGCTC TGGGAGACTG 
AACCTGCAAG AGTTTCATCA CCTCTGGAAG AAGATTAAGA CGTGGCAGAA AATTTTCAAA 
CACTATGACA CAGACCAATC TGGCACCATC AACAGCTACG AGATGCGCAA TGCAGTCAAA 
GATGCAGGCT TCCACCTCAA CAACCAGCTC TACGATATCA TTACCATGCG CTATGCAGAC 
AAGTACATGA ATATTGACTT CGACAGTTTC ATCTGCTGCT TTGTCAGGCT GGAGGGCATG 
TTCAGAGCTT TCAATGCATT TGACAAGGAT GGGGACGGTA TCATCAAACT CAATGTTCTC 
GAGTGGCTGC AGCTCACCAT GTATGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CAPN3 ( XM_015473111.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015328597.1 Bos taurus calpain 3 (CAPN3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa91663
Clone ID Related Accession (Same CDS sequence)	XM_015473111.1 , XM_015473111.2
Accession Version	XM_015473111.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2337bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	calpain-3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104278.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGACCG TCATTAGCGC CTCTGTGGCC CCACGGACAG GGGCTGAGCC CATGTCCCCA 
GGGCCCATCG CCCAGGCAGC CCAGGACAAG GGCACCGAGG CAGGGGGTGG AAACCCAAGT 
GGCATCTACT CAGCCATCAT CAGCCGCAAT TTTCCCATTA TTGGGGTGAA AGAGAAGACA 
TTCGAGCAGC TTCACAAGAA ATGTCTGGAA AAGAAGGTTC TTTTTGTGGA TCCTGAGTTC 
CCACCGGACG AGACCTCCCT GTTTTACAGC CAGAAGTTCC CCATCCAGTT CGTCTGGAAG 
AGACCTCCGG AAATTTGTGA GAATCCCCGA TTTATCGTTG GTGGAGCCAA TAGAACTGAC 
ATCTGCCAAG GAGATCTAGG GGACTGCTGG TTTCTTGCAG CCATCGCTTG CCTGACCTTG 
AACAAGCGTC TGCTTTTCCG GGTCATACCC CATGATCAGA GTTTCACCGA AAACTACGCG 
GGGATTTTTC ACTTCCAGTT CTGGCGCTAT GGAGACTGGG TGGACGTGGT TATTGATGAC 
TGCCTGCCAA CCTACAACAA TCAACTGGTT TTCACCAAAT CCAACCATCG CAATGAGTTC 
TGGAGTGCTC TGCTGGAGAA GGCTTATGCT AAGCTCCATG GTTCGTATGA AGCCCTGAAA 
GGTGGGAACA CTACAGAGGC CATGGAGGAC TTCACGGGAG GAGTGACAGA GTTTTTTGAA 
ATCAAGGATG CTCCCAGAGA CATGTACAAG ATCATGAAGA AAGCCATCGA GAGGGGTTCC 
CTCATGGGCT GCTCCATTGA TGATGGCACA AACATGACCT ATGGAACCTC TCCTTCTGGG 
CTGAAAATGG GGGAGTTGAT TGAGCGGATG GTGAGGAATA TGGATAACTC GCGGCTCAGG 
GACTCAGACC TCATCCCTGA GGGATGCTCA GATGACAGAC CAACTCGGAT GATTGTTCCA 
GTTCAGTTTG AGACAAGAAT GGCCTGTGGG CTGGTCAAAG GCCATGCCTA CTCAGTCACT 
GGGCTGGAGG AGGCCCTGTA CAAGGGTGAG AAAGTGAAGC TGGTGCGGCT GCGGAACCCC 
TGGGGCCAGG TGGAGTGGAA TGGCTCCTGG AGTGACAGCT GGAAGGACTG GAGCTATGTG 
GACAAGGACG AGAAGGCCCG TTTGCAGCAC CAGGTCACTG AGGATGGAGA GTTCTGGATG 
TCCTACGATG ATTTTATCTA CCATTTCACA AAGCTGGAGA TCTGCAACCT CACAGCTGAT 
GCCCTGGAGT CCGACAAGCT TCAGACTTGG ACAGTGTCCG TGAATGAGGG CCGCTGGGTG 
AGGGGCTGCT CTGCCGGAGG CTGCCGCAAC TTCCCAGACA CTTTCTGGAC CAACCCACAG 
TACCGTCTGA AGCTCCTAGA GGAGGACGAC GACCCCGATG ATTCCGAGGT GATCTGTAGT 
TTCCTGGTGG CTCTGATGCA GAAGAACCGG AGGAAGGACC GGAAGCTGGG GGCTAACCTC 
TTCACCATCG GTTTCGCCAT CTACGAGGTT CCCAAAGAGA TGCACGGCAA CAAGCAGCAC 
CTGCAGAAGG ACTTCTTCCT GTACAATGCC TCCAAGGCTA GGAGCAGAAC CTACATCAAC 
ATGCGGGAGG TGTCTGAGCG CTTCCGCCTG CCTCCCAGCG AGTACGTCAT TGTGCCCTCC 
ACTTACGAGC CCCACCAGGA GGGCGAGTTC ATCCTCCGGG TCTTCTCGGA AAAGAGGAAC 
CTCTCTGAGG AAGTTGAGAA TACAATCTCT GTGGATCGGC CAGTGCTAGA GCCTGGCAAC 
ACCGACCAGG AAAGTGAGGA ACAGCGGCAA TTCCGGAATA TTTTCAGGCA GATAGCAGGC 
GATGACATGG AGATCTGCGC AGATGAGCTC AAGAACGTCC TTAACAGAGT TGTGAACAAA 
CATAAGGACC TGAAGACACA AGGCTTCACG CTGGAGTCCT GCCGTAGCAT GATTGCTCTC 
ATGGACACAG ATGGCTCTGG GAGACTGAAC CTGCAAGAGT TTCATCACCT CTGGAAGAAG 
ATTAAGACGT GGCAGAAAAT TTTCAAACAC TATGACACAG ACCAATCTGG CACCATCAAC 
AGCTACGAGA TGCGCAATGC AGTCAAAGAT GCAGGCTTCC ACCTCAACAA CCAGCTCTAC 
GATATCATTA CCATGCGCTA TGCAGACAAG TACATGAATA TTGACTTCGA CAGTTTCATC 
TGCTGCTTTG TCAGGCTGGA GGGCATGTTC AGAGCTTTCA ATGCATTTGA CAAGGATGGG 
GACGGTATCA TCAAACTCAA TGTTCTCGAG TGGCTGCAGC TCACCATGTA TGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015328597.1
CDS	304..2640
Translation	MPTVISASVAPRTGAEPMSPGPIAQAAQDKGTEAGGGNPSGIYSAIISRNFPIIGVKEKTFEQLHKKCLEKKVLFVDPEFPPDETSLFYSQKFPIQFVWKRPPEICENPRFIVGGANRTDICQGDLGDCWFLAAIACLTLNKRLLFRVIPHDQSFTENYAGIFHFQFWRYGDWVDVVIDDCLPTYNNQLVFTKSNHRNEFWSALLEKAYAKLHGSYEALKGGNTTEAMEDFTGGVTEFFEIKDAPRDMYKIMKKAIERGSLMGCSIDDGTNMTYGTSPSGLKMGELIERMVRNMDNSRLRDSDLIPEGCSDDRPTRMIVPVQFETRMACGLVKGHAYSVTGLEEALYKGEKVKLVRLRNPWGQVEWNGSWSDSWKDWSYVDKDEKARLQHQVTEDGEFWMSYDDFIYHFTKLEICNLTADALESDKLQTWTVSVNEGRWVRGCSAGGCRNFPDTFWTNPQYRLKLLEEDDDPDDSEVICSFLVALMQKNRRKDRKLGANLFTIGFAIYEVPKEMHGNKQHLQKDFFLYNASKARSRTYINMREVSERFRLPPSEYVIVPSTYEPHQEGEFILRVFSEKRNLSEEVENTISVDRPVLEPGNTDQESEEQRQFRNIFRQIAGDDMEICADELKNVLNRVVNKHKDLKTQGFTLESCRSMIALMDTDGSGRLNLQEFHHLWKKIKTWQKIFKHYDTDQSGTINSYEMRNAVKDAGFHLNNQLYDIITMRYADKYMNIDFDSFICCFVRLEGMFRAFNAFDKDGDGIIKLNVLEWLQLTMYA

Target ORF information:

RefSeq Version	XM_015473111.1
Organism	Bos taurus(cattle)
Definition	Bos taurus calpain 3 (CAPN3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015473111.1

ORF Insert Sequence:

ATGCCGACCG TCATTAGCGC CTCTGTGGCC CCACGGACAG GGGCTGAGCC CATGTCCCCA 
GGGCCCATCG CCCAGGCAGC CCAGGACAAG GGCACCGAGG CAGGGGGTGG AAACCCAAGT 
GGCATCTACT CAGCCATCAT CAGCCGCAAT TTTCCCATTA TTGGGGTGAA AGAGAAGACA 
TTCGAGCAGC TTCACAAGAA ATGTCTGGAA AAGAAGGTTC TTTTTGTGGA TCCTGAGTTC 
CCACCGGACG AGACCTCCCT GTTTTACAGC CAGAAGTTCC CCATCCAGTT CGTCTGGAAG 
AGACCTCCGG AAATTTGTGA GAATCCCCGA TTTATCGTTG GTGGAGCCAA TAGAACTGAC 
ATCTGCCAAG GAGATCTAGG GGACTGCTGG TTTCTTGCAG CCATCGCTTG CCTGACCTTG 
AACAAGCGTC TGCTTTTCCG GGTCATACCC CATGATCAGA GTTTCACCGA AAACTACGCG 
GGGATTTTTC ACTTCCAGTT CTGGCGCTAT GGAGACTGGG TGGACGTGGT TATTGATGAC 
TGCCTGCCAA CCTACAACAA TCAACTGGTT TTCACCAAAT CCAACCATCG CAATGAGTTC 
TGGAGTGCTC TGCTGGAGAA GGCTTATGCT AAGCTCCATG GTTCGTATGA AGCCCTGAAA 
GGTGGGAACA CTACAGAGGC CATGGAGGAC TTCACGGGAG GAGTGACAGA GTTTTTTGAA 
ATCAAGGATG CTCCCAGAGA CATGTACAAG ATCATGAAGA AAGCCATCGA GAGGGGTTCC 
CTCATGGGCT GCTCCATTGA TGATGGCACA AACATGACCT ATGGAACCTC TCCTTCTGGG 
CTGAAAATGG GGGAGTTGAT TGAGCGGATG GTGAGGAATA TGGATAACTC GCGGCTCAGG 
GACTCAGACC TCATCCCTGA GGGATGCTCA GATGACAGAC CAACTCGGAT GATTGTTCCA 
GTTCAGTTTG AGACAAGAAT GGCCTGTGGG CTGGTCAAAG GCCATGCCTA CTCAGTCACT 
GGGCTGGAGG AGGCCCTGTA CAAGGGTGAG AAAGTGAAGC TGGTGCGGCT GCGGAACCCC 
TGGGGCCAGG TGGAGTGGAA TGGCTCCTGG AGTGACAGCT GGAAGGACTG GAGCTATGTG 
GACAAGGACG AGAAGGCCCG TTTGCAGCAC CAGGTCACTG AGGATGGAGA GTTCTGGATG 
TCCTACGATG ATTTTATCTA CCATTTCACA AAGCTGGAGA TCTGCAACCT CACAGCTGAT 
GCCCTGGAGT CCGACAAGCT TCAGACTTGG ACAGTGTCCG TGAATGAGGG CCGCTGGGTG 
AGGGGCTGCT CTGCCGGAGG CTGCCGCAAC TTCCCAGACA CTTTCTGGAC CAACCCACAG 
TACCGTCTGA AGCTCCTAGA GGAGGACGAC GACCCCGATG ATTCCGAGGT GATCTGTAGT 
TTCCTGGTGG CTCTGATGCA GAAGAACCGG AGGAAGGACC GGAAGCTGGG GGCTAACCTC 
TTCACCATCG GTTTCGCCAT CTACGAGGTT CCCAAAGAGA TGCACGGCAA CAAGCAGCAC 
CTGCAGAAGG ACTTCTTCCT GTACAATGCC TCCAAGGCTA GGAGCAGAAC CTACATCAAC 
ATGCGGGAGG TGTCTGAGCG CTTCCGCCTG CCTCCCAGCG AGTACGTCAT TGTGCCCTCC 
ACTTACGAGC CCCACCAGGA GGGCGAGTTC ATCCTCCGGG TCTTCTCGGA AAAGAGGAAC 
CTCTCTGAGG AAGTTGAGAA TACAATCTCT GTGGATCGGC CAGTGCTAGA GCCTGGCAAC 
ACCGACCAGG AAAGTGAGGA ACAGCGGCAA TTCCGGAATA TTTTCAGGCA GATAGCAGGC 
GATGACATGG AGATCTGCGC AGATGAGCTC AAGAACGTCC TTAACAGAGT TGTGAACAAA 
CATAAGGACC TGAAGACACA AGGCTTCACG CTGGAGTCCT GCCGTAGCAT GATTGCTCTC 
ATGGACACAG ATGGCTCTGG GAGACTGAAC CTGCAAGAGT TTCATCACCT CTGGAAGAAG 
ATTAAGACGT GGCAGAAAAT TTTCAAACAC TATGACACAG ACCAATCTGG CACCATCAAC 
AGCTACGAGA TGCGCAATGC AGTCAAAGAT GCAGGCTTCC ACCTCAACAA CCAGCTCTAC 
GATATCATTA CCATGCGCTA TGCAGACAAG TACATGAATA TTGACTTCGA CAGTTTCATC 
TGCTGCTTTG TCAGGCTGGA GGGCATGTTC AGAGCTTTCA ATGCATTTGA CAAGGATGGG 
GACGGTATCA TCAAACTCAA TGTTCTCGAG TGGCTGCAGC TCACCATGTA TGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CAPN3 ( XM_015473111.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015328597.1 Bos taurus calpain 3 (CAPN3), transcript variant X2, mRNA.

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CloneID	OBa91663
Clone ID Related Accession (Same CDS sequence)	XM_015473111.1 , XM_015473111.2
Accession Version	XM_015473111.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2337bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	calpain-3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015473111.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGACCG TCATTAGCGC CTCTGTGGCC CCACGGACAG GGGCTGAGCC CATGTCCCCA 
GGGCCCATCG CCCAGGCAGC CCAGGACAAG GGCACCGAGG CAGGGGGTGG AAACCCAAGT 
GGCATCTACT CAGCCATCAT CAGCCGCAAT TTTCCCATTA TTGGGGTGAA AGAGAAGACA 
TTCGAGCAGC TTCACAAGAA ATGTCTGGAA AAGAAGGTTC TTTTTGTGGA TCCTGAGTTC 
CCACCGGACG AGACCTCCCT GTTTTACAGC CAGAAGTTCC CCATCCAGTT CGTCTGGAAG 
AGACCTCCGG AAATTTGTGA GAATCCCCGA TTTATCGTTG GTGGAGCCAA TAGAACTGAC 
ATCTGCCAAG GAGATCTAGG GGACTGCTGG TTTCTTGCAG CCATCGCTTG CCTGACCTTG 
AACAAGCGTC TGCTTTTCCG GGTCATACCC CATGATCAGA GTTTCACCGA AAACTACGCG 
GGGATTTTTC ACTTCCAGTT CTGGCGCTAT GGAGACTGGG TGGACGTGGT TATTGATGAC 
TGCCTGCCAA CCTACAACAA TCAACTGGTT TTCACCAAAT CCAACCATCG CAATGAGTTC 
TGGAGTGCTC TGCTGGAGAA GGCTTATGCT AAGCTCCATG GTTCGTATGA AGCCCTGAAA 
GGTGGGAACA CTACAGAGGC CATGGAGGAC TTCACGGGAG GAGTGACAGA GTTTTTTGAA 
ATCAAGGATG CTCCCAGAGA CATGTACAAG ATCATGAAGA AAGCCATCGA GAGGGGTTCC 
CTCATGGGCT GCTCCATTGA TGATGGCACA AACATGACCT ATGGAACCTC TCCTTCTGGG 
CTGAAAATGG GGGAGTTGAT TGAGCGGATG GTGAGGAATA TGGATAACTC GCGGCTCAGG 
GACTCAGACC TCATCCCTGA GGGATGCTCA GATGACAGAC CAACTCGGAT GATTGTTCCA 
GTTCAGTTTG AGACAAGAAT GGCCTGTGGG CTGGTCAAAG GCCATGCCTA CTCAGTCACT 
GGGCTGGAGG AGGCCCTGTA CAAGGGTGAG AAAGTGAAGC TGGTGCGGCT GCGGAACCCC 
TGGGGCCAGG TGGAGTGGAA TGGCTCCTGG AGTGACAGCT GGAAGGACTG GAGCTATGTG 
GACAAGGACG AGAAGGCCCG TTTGCAGCAC CAGGTCACTG AGGATGGAGA GTTCTGGATG 
TCCTACGATG ATTTTATCTA CCATTTCACA AAGCTGGAGA TCTGCAACCT CACAGCTGAT 
GCCCTGGAGT CCGACAAGCT TCAGACTTGG ACAGTGTCCG TGAATGAGGG CCGCTGGGTG 
AGGGGCTGCT CTGCCGGAGG CTGCCGCAAC TTCCCAGACA CTTTCTGGAC CAACCCACAG 
TACCGTCTGA AGCTCCTAGA GGAGGACGAC GACCCCGATG ATTCCGAGGT GATCTGTAGT 
TTCCTGGTGG CTCTGATGCA GAAGAACCGG AGGAAGGACC GGAAGCTGGG GGCTAACCTC 
TTCACCATCG GTTTCGCCAT CTACGAGGTT CCCAAAGAGA TGCACGGCAA CAAGCAGCAC 
CTGCAGAAGG ACTTCTTCCT GTACAATGCC TCCAAGGCTA GGAGCAGAAC CTACATCAAC 
ATGCGGGAGG TGTCTGAGCG CTTCCGCCTG CCTCCCAGCG AGTACGTCAT TGTGCCCTCC 
ACTTACGAGC CCCACCAGGA GGGCGAGTTC ATCCTCCGGG TCTTCTCGGA AAAGAGGAAC 
CTCTCTGAGG AAGTTGAGAA TACAATCTCT GTGGATCGGC CAGTGCTAGA GCCTGGCAAC 
ACCGACCAGG AAAGTGAGGA ACAGCGGCAA TTCCGGAATA TTTTCAGGCA GATAGCAGGC 
GATGACATGG AGATCTGCGC AGATGAGCTC AAGAACGTCC TTAACAGAGT TGTGAACAAA 
CATAAGGACC TGAAGACACA AGGCTTCACG CTGGAGTCCT GCCGTAGCAT GATTGCTCTC 
ATGGACACAG ATGGCTCTGG GAGACTGAAC CTGCAAGAGT TTCATCACCT CTGGAAGAAG 
ATTAAGACGT GGCAGAAAAT TTTCAAACAC TATGACACAG ACCAATCTGG CACCATCAAC 
AGCTACGAGA TGCGCAATGC AGTCAAAGAT GCAGGCTTCC ACCTCAACAA CCAGCTCTAC 
GATATCATTA CCATGCGCTA TGCAGACAAG TACATGAATA TTGACTTCGA CAGTTTCATC 
TGCTGCTTTG TCAGGCTGGA GGGCATGTTC AGAGCTTTCA ATGCATTTGA CAAGGATGGG 
GACGGTATCA TCAAACTCAA TGTTCTCGAG TGGCTGCAGC TCACCATGTA TGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015328597.1
CDS	304..2640
Translation	MPTVISASVAPRTGAEPMSPGPIAQAAQDKGTEAGGGNPSGIYSAIISRNFPIIGVKEKTFEQLHKKCLEKKVLFVDPEFPPDETSLFYSQKFPIQFVWKRPPEICENPRFIVGGANRTDICQGDLGDCWFLAAIACLTLNKRLLFRVIPHDQSFTENYAGIFHFQFWRYGDWVDVVIDDCLPTYNNQLVFTKSNHRNEFWSALLEKAYAKLHGSYEALKGGNTTEAMEDFTGGVTEFFEIKDAPRDMYKIMKKAIERGSLMGCSIDDGTNMTYGTSPSGLKMGELIERMVRNMDNSRLRDSDLIPEGCSDDRPTRMIVPVQFETRMACGLVKGHAYSVTGLEEALYKGEKVKLVRLRNPWGQVEWNGSWSDSWKDWSYVDKDEKARLQHQVTEDGEFWMSYDDFIYHFTKLEICNLTADALESDKLQTWTVSVNEGRWVRGCSAGGCRNFPDTFWTNPQYRLKLLEEDDDPDDSEVICSFLVALMQKNRRKDRKLGANLFTIGFAIYEVPKEMHGNKQHLQKDFFLYNASKARSRTYINMREVSERFRLPPSEYVIVPSTYEPHQEGEFILRVFSEKRNLSEEVENTISVDRPVLEPGNTDQESEEQRQFRNIFRQIAGDDMEICADELKNVLNRVVNKHKDLKTQGFTLESCRSMIALMDTDGSGRLNLQEFHHLWKKIKTWQKIFKHYDTDQSGTINSYEMRNAVKDAGFHLNNQLYDIITMRYADKYMNIDFDSFICCFVRLEGMFRAFNAFDKDGDGIIKLNVLEWLQLTMYA

Target ORF information:

RefSeq Version	XM_015473111.2
Organism	Bos taurus(cattle)
Definition	Bos taurus calpain 3 (CAPN3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015473111.2

ORF Insert Sequence:

ATGCCGACCG TCATTAGCGC CTCTGTGGCC CCACGGACAG GGGCTGAGCC CATGTCCCCA 
GGGCCCATCG CCCAGGCAGC CCAGGACAAG GGCACCGAGG CAGGGGGTGG AAACCCAAGT 
GGCATCTACT CAGCCATCAT CAGCCGCAAT TTTCCCATTA TTGGGGTGAA AGAGAAGACA 
TTCGAGCAGC TTCACAAGAA ATGTCTGGAA AAGAAGGTTC TTTTTGTGGA TCCTGAGTTC 
CCACCGGACG AGACCTCCCT GTTTTACAGC CAGAAGTTCC CCATCCAGTT CGTCTGGAAG 
AGACCTCCGG AAATTTGTGA GAATCCCCGA TTTATCGTTG GTGGAGCCAA TAGAACTGAC 
ATCTGCCAAG GAGATCTAGG GGACTGCTGG TTTCTTGCAG CCATCGCTTG CCTGACCTTG 
AACAAGCGTC TGCTTTTCCG GGTCATACCC CATGATCAGA GTTTCACCGA AAACTACGCG 
GGGATTTTTC ACTTCCAGTT CTGGCGCTAT GGAGACTGGG TGGACGTGGT TATTGATGAC 
TGCCTGCCAA CCTACAACAA TCAACTGGTT TTCACCAAAT CCAACCATCG CAATGAGTTC 
TGGAGTGCTC TGCTGGAGAA GGCTTATGCT AAGCTCCATG GTTCGTATGA AGCCCTGAAA 
GGTGGGAACA CTACAGAGGC CATGGAGGAC TTCACGGGAG GAGTGACAGA GTTTTTTGAA 
ATCAAGGATG CTCCCAGAGA CATGTACAAG ATCATGAAGA AAGCCATCGA GAGGGGTTCC 
CTCATGGGCT GCTCCATTGA TGATGGCACA AACATGACCT ATGGAACCTC TCCTTCTGGG 
CTGAAAATGG GGGAGTTGAT TGAGCGGATG GTGAGGAATA TGGATAACTC GCGGCTCAGG 
GACTCAGACC TCATCCCTGA GGGATGCTCA GATGACAGAC CAACTCGGAT GATTGTTCCA 
GTTCAGTTTG AGACAAGAAT GGCCTGTGGG CTGGTCAAAG GCCATGCCTA CTCAGTCACT 
GGGCTGGAGG AGGCCCTGTA CAAGGGTGAG AAAGTGAAGC TGGTGCGGCT GCGGAACCCC 
TGGGGCCAGG TGGAGTGGAA TGGCTCCTGG AGTGACAGCT GGAAGGACTG GAGCTATGTG 
GACAAGGACG AGAAGGCCCG TTTGCAGCAC CAGGTCACTG AGGATGGAGA GTTCTGGATG 
TCCTACGATG ATTTTATCTA CCATTTCACA AAGCTGGAGA TCTGCAACCT CACAGCTGAT 
GCCCTGGAGT CCGACAAGCT TCAGACTTGG ACAGTGTCCG TGAATGAGGG CCGCTGGGTG 
AGGGGCTGCT CTGCCGGAGG CTGCCGCAAC TTCCCAGACA CTTTCTGGAC CAACCCACAG 
TACCGTCTGA AGCTCCTAGA GGAGGACGAC GACCCCGATG ATTCCGAGGT GATCTGTAGT 
TTCCTGGTGG CTCTGATGCA GAAGAACCGG AGGAAGGACC GGAAGCTGGG GGCTAACCTC 
TTCACCATCG GTTTCGCCAT CTACGAGGTT CCCAAAGAGA TGCACGGCAA CAAGCAGCAC 
CTGCAGAAGG ACTTCTTCCT GTACAATGCC TCCAAGGCTA GGAGCAGAAC CTACATCAAC 
ATGCGGGAGG TGTCTGAGCG CTTCCGCCTG CCTCCCAGCG AGTACGTCAT TGTGCCCTCC 
ACTTACGAGC CCCACCAGGA GGGCGAGTTC ATCCTCCGGG TCTTCTCGGA AAAGAGGAAC 
CTCTCTGAGG AAGTTGAGAA TACAATCTCT GTGGATCGGC CAGTGCTAGA GCCTGGCAAC 
ACCGACCAGG AAAGTGAGGA ACAGCGGCAA TTCCGGAATA TTTTCAGGCA GATAGCAGGC 
GATGACATGG AGATCTGCGC AGATGAGCTC AAGAACGTCC TTAACAGAGT TGTGAACAAA 
CATAAGGACC TGAAGACACA AGGCTTCACG CTGGAGTCCT GCCGTAGCAT GATTGCTCTC 
ATGGACACAG ATGGCTCTGG GAGACTGAAC CTGCAAGAGT TTCATCACCT CTGGAAGAAG 
ATTAAGACGT GGCAGAAAAT TTTCAAACAC TATGACACAG ACCAATCTGG CACCATCAAC 
AGCTACGAGA TGCGCAATGC AGTCAAAGAT GCAGGCTTCC ACCTCAACAA CCAGCTCTAC 
GATATCATTA CCATGCGCTA TGCAGACAAG TACATGAATA TTGACTTCGA CAGTTTCATC 
TGCTGCTTTG TCAGGCTGGA GGGCATGTTC AGAGCTTTCA ATGCATTTGA CAAGGATGGG 
GACGGTATCA TCAAACTCAA TGTTCTCGAG TGGCTGCAGC TCACCATGTA TGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Characterization of a novel CAPN3 transcript generated by alternative splicing in cattle.
Genetics and molecular research : GMR14(1)457-63(2015 Jan)
Liu SY,Jiang H,Yuan B,Gao Y,Dai LS,Zhang JB

Production and processing studies on calpain-system gene markers for beef tenderness: consumer assessments of eating quality.
Journal of animal science90(8)2850-60(2012 Aug)
Robinson DL,Cafe LM,McIntyre BL,Geesink GH,Barendse W,Pethick DW,Thompson JM,Polkinghorne R,Greenwood PL

Calpain3 is expressed in a proteolitically active form in papillomavirus-associated urothelial tumors of the urinary bladder in cattle.
PloS one5(4)e10299(2010 Apr)
Roperto S,De Tullio R,Raso C,Stifanese R,Russo V,Gaspari M,Borzacchiello G,Averna M,Paciello O,Cuda G,Roperto F

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

Variation at the Calpain 3 gene is associated with meat tenderness in zebu and composite breeds of cattle.
BMC genetics941(2008 Jul)
Barendse W,Harrison BE,Bunch RJ,Thomas MB

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CAPN3 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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FAX

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