Capn3 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Capn3
Entrez Gene ID	29155
Full Name	calpain 3
Synonyms	Lp82,Lp84,Lp85,p94
General protein information	Preferred Names calpain 3 Names calpain-3 CANP 3 calcium-activated neutral proteinase 3 calpain L3 calpain p94 muscle-specific calcium-activated neutral protease 3
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 3 Map Location: 3q35
Summary	calcium-activated protease; alternative splicing produces different protein products; may be involved in astrocyte plasticity and in eye development and pathology [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_006234728.3	XP_006234790.1	calpain-3 isoform X3
XM_017591508.1	XP_017446997.1	calpain-3 isoform X2
XM_008762081.2	XP_008760303.1	calpain-3 isoform X4
NM_017117.1	NP_058813.1	calpain-3
XM_008762080.2	XP_008760302.1	calpain-3 isoform X1
XM_006234729.3	XP_006234791.1	calpain-3 isoform X5

Pathways from BioSystems

Homologies

Xenopus tropicalis (tropical clawed frog)	LOC100497532	XP_002935381.2
Homo sapiens (human)	CAPN3	NP_000061.1
Mus musculus (house mouse)	Capn3	NP_031627.2
Rattus norvegicus (Norway rat)	Capn3	NP_058813.1
Canis lupus familiaris (dog)	CAPN3	XP_005638349.1
Gallus gallus (chicken)	CAPN3	NP_001004405.1
	CAPN3	XP_001103220.1
Danio rerio (zebrafish)	capn3a	NP_001004571.1
Bos taurus (cattle)	CAPN3	NP_776685.1

Gene Ontology
Bibliography

Related articles in PubMed

Calpain 3 Expression Pattern during Gastrocnemius Muscle Atrophy and Regeneration Following Sciatic Nerve Injury in Rats.
Wu R, Yan Y, Yao J, Liu Y, Zhao J, Liu M
International journal of molecular sciences16(11)26927-35(2015 Nov)

Def defines a conserved nucleolar pathway that leads p53 to proteasome-independent degradation.
Tao T, Shi H, Guan Y, Huang D, Chen Y, Lane DP, Chen J, Peng J
Cell research23(5)620-34(2013 May)

Rbfox1 downregulation and altered calpain 3 splicing by FRG1 in a mouse model of Facioscapulohumeral muscular dystrophy (FSHD).
Pistoni M, Shiue L, Cline MS, Bortolanza S, Neguembor MV, Xynos A, Ares M Jr, Gabellini D
PLoS genetics9(1)e1003186(2013)

Calpain 3 is a rapid-action, unidirectional proteolytic switch central to muscle remodeling.
de Morr?e A, Lutje Hulsik D, Impagliazzo A, van Haagen HH, de Galan P, van Remoortere A, 't Hoen PA, van Ommen GB, Frants RR, van der Maarel SM
PloS one5(8)e11940(2010 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

the expression pattern of the active CAPN3 protein is linked to muscle atrophy and regeneration following denervation
Title: Calpain 3 Expression Pattern during Gastrocnemius Muscle Atrophy and Regeneration Following Sciatic Nerve Injury in Rats.

roles for Na(+) dependence of p94
Title: Skeletal muscle-specific calpain is an intracellular Na+-dependent protease.

calpain-3 is not responsible for Ca(2+)-induced disruption of EC coupling, but that mu-calpain is a plausible candidate
Title: Involvement of calpains in Ca2+-induced disruption of excitation-contraction coupling in mammalian skeletal muscle fibers.

calpain-3 remains quiescent with normal exercise but is activated following eccentric (stretching) contractions, when resting [Ca(2+)] is elevated
Title: Endogenous calpain-3 activation is primarily governed by small increases in resting cytoplasmic [Ca2+] and is not dependent on stretch.

All calpain-3 is bound tightly within a fibre, whereas most mu-calpain ( approximately 0.2 microm) is initially freely diffusible in the cytoplasm at resting [Ca(2+)] but binds within seconds at high [Ca(2+)].
Title: Ca2+ activation of diffusible and bound pools of mu-calpain in rat skeletal muscle.

The following Capn3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Capn3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa02908	NM_017117.1 Latest version!	Rattus norvegicus calpain 3 (Capn3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$391.30 $559.00
ORa26201	XM_006234728.3 Latest version!	Rattus norvegicus calpain 3 (Capn3), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
ORa26202	XM_006234729.3 Latest version!	Rattus norvegicus calpain 3 (Capn3), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
ORa43223	XM_008762080.2 Latest version!	Rattus norvegicus calpain 3 (Capn3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
ORa43224	XM_008762081.2 Latest version!	Rattus norvegicus calpain 3 (Capn3), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
ORa60285	XM_017591508.1 Latest version!	Rattus norvegicus calpain 3 (Capn3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Capn3 ( NM_017117.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_058813.1 Rattus norvegicus calpain 3 (Capn3), mRNA.

Price & Availability↑

CloneID	ORa02908
Clone ID Related Accession (Same CDS sequence)	NM_017117.1
Accession Version	NM_017117.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2466bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Rattus norvegicus(Norway rat)
Product	calpain-3
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from J05121.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: J05121.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2689600, SAMEA2689602 [ECO:0000348] ##Evidence-Data-END##

ATGCCGACCG TTATTAGTCC AACTGTGGCC CCAAGGACAG GAGCTGAGCC CAGGTCCCCA 
GGGCCAGTTC CTCACCCAGC TCAAGGCAAG ACCACTGAGG CTGGAGGTGG ACACCCGGGT 
GGCATCTATT CAGCCATCAT CAGCCGCAAT TTTCCGATCA TTGGTGTGAA AGAGAAGACA 
TTCGAGCAGC TCCACAAGAA GTGCCTAGAG AAGAAAGTTC TTTACCTGGA TCCCGAGTTC 
CCACCGGATG AGACCTCTCT CTTTTACAGC CAGAAGTTCC CCATCCAGTT CGTCTGGAAG 
AGACCTCCGG AAATTTGTGA GAATCCCCGA TTTATCATTG GTGGAGCCAA CAGGACTGAC 
ATCTGCCAAG GAGATCTAGG GGACTGCTGG CTTCTTGCAG CCATTGCCTG TCTGACCCTG 
AATGAGCGAC TGCTTTTCCG AGTTATACCT CATGATCAAA GTTTCACTGA AAACTACGCA 
GGGATCTTCC ACTTCCAGTT CTGGCGCTAT GGAGACTGGG TAGATGTGGT TATTGACGAC 
TGTCTGCCGA CATACAACAA CCAGCTGGTC TTCACCAAAT CCAACCACCG CAATGAGTTC 
TGGAGTGCTC TACTGGAGAA AGCATATGCC AAGCTCCATG GTTCCTATGA AGCTCTGAAA 
GGTGGGAACA CCACAGAAGC CATGGAGGAC TTCACAGGAG GGGTGACAGA GTTTTTTGAG 
ATCAAGGATG CTCCGAGTGA CATGTACAAG ATCATGAGGA AAGCTATCGA GAGAGGCTCC 
CTCATGGGCT GCTCCATTGA TGATGGCACC AACATGACTT ATGGAACCTC TCCTTCTGGT 
CTGAACATGG GGGAATTGAT TGCGCGGATG GTGAGAAATA TGGATAACTC GCTGCTCAGA 
GACTCAGACC TGGACCCCAG GGCCTCAGAT GACAGACCGT CACGGACAAT TGTTCCGGTG 
CAGTATGAAA CAAGAATGGC CTGTGGACTG GTGAAAGGGC ACGCCTATTC AGTCACTGGG 
CTGGAGGAGG CCCTGTTCAA AGGCGAGAAG GTGAAGCTGG TGCGGCTGCG GAACCCCTGG 
GGCCAGGTGG AGTGGAACGG CTCTTGGAGT GATGGTTGGA AGGACTGGAG CTTTGTGGAC 
AAAGACGAGA AGGCCCGTCT GCAGCACCAG GTCACCGAGG ATGGAGAGTT CTGGATGTCA 
TATGATGACT TTGTCTACCA TTTCACAAAG CTGGAGATCT GCAACCTCAC AGCTGATGCC 
CTGGAGTCCG ATAAGCTTCA GACCTGGACA GTGTCTGTAA ATGAGGGCCG CTGGGTGAGG 
GGCTGTTCTG CTGGAGGCTG CCGGAACTTC CCAGACACTT TCTGGACCAA CCCGCAGTAC 
CGTCTCAAGC TCCTGGAGGA GGATGATGAC CCTGATGACT CTGAGGTGAT TTGCAGCTTC 
CTCGTGGCTC TGATGCAGAA AAATCGGCGC AAGGACCGGA AGCTGGGGGC CAACCTCTTC 
ACCATTGGCT TCGCTATCTA CGAGGTTCCC AAAGAGATGC ACGGGAATAA GCAACACCTG 
CAGAAGGACT TCTTCTTGTA CAATGCCTCC AAGGCCAGGA GCAAAACCTA CATCAACATG 
CGGGAGGTGT CCCAGCGCTT CCGCCTGCCG CCCAGCGAGT ATGTCATTGT CCCCTCCACT 
TACGAGCCCC ATCAGGAGGG GGAATTCATC CTCCGGGTCT TCTCTGAAAA GAGGAATCTC 
TCTGAGGAAG CTGAGAATAC AATCTCTGTG GACCGGCCAG TGAAAAAGAA AAAAAACAAG 
CCCATCATCT TCGTTTCAGA CAGAGCAAAC AGCAACAAGG AGCTGGGTGT GGACCAGGAG 
GCAGAGGAGG GCAAAGACAA AACAGGGCCG GATAAACAAG GGGAAAGCCC ACAGCCACGG 
CCTGGCCACA CAGACCAGGA GAGTGAGGAG CAGCAGCAAT TCCGGAACAT CTTCAGGCAG 
ATTGCAGGCG ACGACATGGA GATCTGTGCG GATGAACTCA AGAATGTCCT TAATACGGTG 
GTGAACAAAC ACAAGGACCT GAAGACACAA GGGTTCACTC TGGAGTCCTG CAGAAGCATG 
ATAGCTCTCA TGGATACAGA TGGCTCTGGG AGACTGAATC TTCAAGAGTT CCATCACCTC 
TGGAAAAAGA TCAAGGCCTG GCAGAAAATC TTCAAACACT ATGACACTGA CCATTCTGGT 
ACCATCAATA GCTATGAGAT GCGAAATGCA GTCAATGATG CAGGCTTCCA TCTCAACAGC 
CAACTCTATG ACATCATCAC CATGCGCTAC GCAGACAAAC ACATGAACAT CGACTTTGAC 
AGCTTCATCT GCTGCTTCGT CAGGCTGGAA GGGATGTTCA GAGCTTTTCA CGCATTTGAC 
AAGGATGGAG ATGGCATCAT CAAACTGAAC GTACTTGAGT GGCTGCAGCT TACCATGTAT 
GCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_058813.1
CDS	302..2767
Translation	MPTVISPTVAPRTGAEPRSPGPVPHPAQGKTTEAGGGHPGGIYSAIISRNFPIIGVKEKTFEQLHKKCLEKKVLYLDPEFPPDETSLFYSQKFPIQFVWKRPPEICENPRFIIGGANRTDICQGDLGDCWLLAAIACLTLNERLLFRVIPHDQSFTENYAGIFHFQFWRYGDWVDVVIDDCLPTYNNQLVFTKSNHRNEFWSALLEKAYAKLHGSYEALKGGNTTEAMEDFTGGVTEFFEIKDAPSDMYKIMRKAIERGSLMGCSIDDGTNMTYGTSPSGLNMGELIARMVRNMDNSLLRDSDLDPRASDDRPSRTIVPVQYETRMACGLVKGHAYSVTGLEEALFKGEKVKLVRLRNPWGQVEWNGSWSDGWKDWSFVDKDEKARLQHQVTEDGEFWMSYDDFVYHFTKLEICNLTADALESDKLQTWTVSVNEGRWVRGCSAGGCRNFPDTFWTNPQYRLKLLEEDDDPDDSEVICSFLVALMQKNRRKDRKLGANLFTIGFAIYEVPKEMHGNKQHLQKDFFLYNASKARSKTYINMREVSQRFRLPPSEYVIVPSTYEPHQEGEFILRVFSEKRNLSEEAENTISVDRPVKKKKNKPIIFVSDRANSNKELGVDQEAEEGKDKTGPDKQGESPQPRPGHTDQESEEQQQFRNIFRQIAGDDMEICADELKNVLNTVVNKHKDLKTQGFTLESCRSMIALMDTDGSGRLNLQEFHHLWKKIKAWQKIFKHYDTDHSGTINSYEMRNAVNDAGFHLNSQLYDIITMRYADKHMNIDFDSFICCFVRLEGMFRAFHAFDKDGDGIIKLNVLEWLQLTMYA

Target ORF information:

RefSeq Version	NM_017117.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus calpain 3 (Capn3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_017117.1

ORF Insert Sequence:

ATGCCGACCG TTATTAGTCC AACTGTGGCC CCAAGGACAG GAGCTGAGCC CAGGTCCCCA 
GGGCCAGTTC CTCACCCAGC TCAAGGCAAG ACCACTGAGG CTGGAGGTGG ACACCCGGGT 
GGCATCTATT CAGCCATCAT CAGCCGCAAT TTTCCGATCA TTGGTGTGAA AGAGAAGACA 
TTCGAGCAGC TCCACAAGAA GTGCCTAGAG AAGAAAGTTC TTTACCTGGA TCCCGAGTTC 
CCACCGGATG AGACCTCTCT CTTTTACAGC CAGAAGTTCC CCATCCAGTT CGTCTGGAAG 
AGACCTCCGG AAATTTGTGA GAATCCCCGA TTTATCATTG GTGGAGCCAA CAGGACTGAC 
ATCTGCCAAG GAGATCTAGG GGACTGCTGG CTTCTTGCAG CCATTGCCTG TCTGACCCTG 
AATGAGCGAC TGCTTTTCCG AGTTATACCT CATGATCAAA GTTTCACTGA AAACTACGCA 
GGGATCTTCC ACTTCCAGTT CTGGCGCTAT GGAGACTGGG TAGATGTGGT TATTGACGAC 
TGTCTGCCGA CATACAACAA CCAGCTGGTC TTCACCAAAT CCAACCACCG CAATGAGTTC 
TGGAGTGCTC TACTGGAGAA AGCATATGCC AAGCTCCATG GTTCCTATGA AGCTCTGAAA 
GGTGGGAACA CCACAGAAGC CATGGAGGAC TTCACAGGAG GGGTGACAGA GTTTTTTGAG 
ATCAAGGATG CTCCGAGTGA CATGTACAAG ATCATGAGGA AAGCTATCGA GAGAGGCTCC 
CTCATGGGCT GCTCCATTGA TGATGGCACC AACATGACTT ATGGAACCTC TCCTTCTGGT 
CTGAACATGG GGGAATTGAT TGCGCGGATG GTGAGAAATA TGGATAACTC GCTGCTCAGA 
GACTCAGACC TGGACCCCAG GGCCTCAGAT GACAGACCGT CACGGACAAT TGTTCCGGTG 
CAGTATGAAA CAAGAATGGC CTGTGGACTG GTGAAAGGGC ACGCCTATTC AGTCACTGGG 
CTGGAGGAGG CCCTGTTCAA AGGCGAGAAG GTGAAGCTGG TGCGGCTGCG GAACCCCTGG 
GGCCAGGTGG AGTGGAACGG CTCTTGGAGT GATGGTTGGA AGGACTGGAG CTTTGTGGAC 
AAAGACGAGA AGGCCCGTCT GCAGCACCAG GTCACCGAGG ATGGAGAGTT CTGGATGTCA 
TATGATGACT TTGTCTACCA TTTCACAAAG CTGGAGATCT GCAACCTCAC AGCTGATGCC 
CTGGAGTCCG ATAAGCTTCA GACCTGGACA GTGTCTGTAA ATGAGGGCCG CTGGGTGAGG 
GGCTGTTCTG CTGGAGGCTG CCGGAACTTC CCAGACACTT TCTGGACCAA CCCGCAGTAC 
CGTCTCAAGC TCCTGGAGGA GGATGATGAC CCTGATGACT CTGAGGTGAT TTGCAGCTTC 
CTCGTGGCTC TGATGCAGAA AAATCGGCGC AAGGACCGGA AGCTGGGGGC CAACCTCTTC 
ACCATTGGCT TCGCTATCTA CGAGGTTCCC AAAGAGATGC ACGGGAATAA GCAACACCTG 
CAGAAGGACT TCTTCTTGTA CAATGCCTCC AAGGCCAGGA GCAAAACCTA CATCAACATG 
CGGGAGGTGT CCCAGCGCTT CCGCCTGCCG CCCAGCGAGT ATGTCATTGT CCCCTCCACT 
TACGAGCCCC ATCAGGAGGG GGAATTCATC CTCCGGGTCT TCTCTGAAAA GAGGAATCTC 
TCTGAGGAAG CTGAGAATAC AATCTCTGTG GACCGGCCAG TGAAAAAGAA AAAAAACAAG 
CCCATCATCT TCGTTTCAGA CAGAGCAAAC AGCAACAAGG AGCTGGGTGT GGACCAGGAG 
GCAGAGGAGG GCAAAGACAA AACAGGGCCG GATAAACAAG GGGAAAGCCC ACAGCCACGG 
CCTGGCCACA CAGACCAGGA GAGTGAGGAG CAGCAGCAAT TCCGGAACAT CTTCAGGCAG 
ATTGCAGGCG ACGACATGGA GATCTGTGCG GATGAACTCA AGAATGTCCT TAATACGGTG 
GTGAACAAAC ACAAGGACCT GAAGACACAA GGGTTCACTC TGGAGTCCTG CAGAAGCATG 
ATAGCTCTCA TGGATACAGA TGGCTCTGGG AGACTGAATC TTCAAGAGTT CCATCACCTC 
TGGAAAAAGA TCAAGGCCTG GCAGAAAATC TTCAAACACT ATGACACTGA CCATTCTGGT 
ACCATCAATA GCTATGAGAT GCGAAATGCA GTCAATGATG CAGGCTTCCA TCTCAACAGC 
CAACTCTATG ACATCATCAC CATGCGCTAC GCAGACAAAC ACATGAACAT CGACTTTGAC 
AGCTTCATCT GCTGCTTCGT CAGGCTGGAA GGGATGTTCA GAGCTTTTCA CGCATTTGAC 
AAGGATGGAG ATGGCATCAT CAAACTGAAC GTACTTGAGT GGCTGCAGCT TACCATGTAT 
GCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Capn3 ( XM_006234728.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006234790.1 Rattus norvegicus calpain 3 (Capn3), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa26201
Clone ID Related Accession (Same CDS sequence)	XM_006234728.3
Accession Version	XM_006234728.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2358bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	calpain-3 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006234728.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCTACC TGCTGCCGGG ATTCTTCTGT GACAGAGTGA TCAGAGAAAG GGACAGGAGA 
AATGGAGAGG GCACCGTCTC ACAGCCTCTC AAGTTTGAGG GGCAGGATTT TGTCGTTCTC 
AAACAACGGT GTCTGGCTCA GAAGTGCCTC TTTGAAGATC GAGTCTTCCC AGCAGGTACA 
CAGGCCCTTG GCTCACATGA GCTGAGCCAG AAAGCCAAGA TGAAGGCCAT CACTTGGAAG 
AGGCCAAAGG AAATTTGTGA GAATCCCCGA TTTATCATTG GTGGAGCCAA CAGGACTGAC 
ATCTGCCAAG GAGATCTAGG GGACTGCTGG TTTCTTGCAG CCATTGCCTG TCTGACCCTG 
AATGAGCGAC TGCTTTTCCG AGTTATACCT CATGATCAAA GTTTCACTGA AAACTACGCA 
GGGATCTTCC ACTTCCAGTT CTGGCGCTAT GGAGACTGGG TAGATGTGGT TATTGACGAC 
TGTCTGCCGA CATACAACAA CCAGCTGGTC TTCACCAAAT CCAACCACCG CAATGAGTTC 
TGGAGTGCTC TACTGGAGAA AGCATATGCC AAGCTCCATG GTTCCTATGA AGCTCTGAAA 
GGTGGGAACA CCACAGAAGC CATGGAGGAC TTCACAGGAG GGGTGACAGA GTTTTTTGAG 
ATCAAGGATG CTCCGAGTGA CATGTACAAG ATCATGAGGA AAGCTATCGA GAGAGGCTCC 
CTCATGGGCT GCTCCATTGA TGATGGCACC AACATGACTT ATGGAACCTC TCCTTCTGGT 
CTGAACATGG GGGAATTGAT TGCGCGGATG GTGAGAAATA TGGATAACTC GCTGCTCAGA 
GACTCAGACC TGGACCCCAG GGCCTCAGAT GACAGACCGT CACGGACAAT TGTTCCGGTG 
CAGTATGAAA CAAGAATGGC CTGTGGACTG GTGAAAGGGC ACGCCTATTC AGTCACTGGG 
CTGGAGGAGG CCCTGTTCAA AGGCGAGAAG GTGAAGCTGG TGCGGCTGCG GAACCCCTGG 
GGCCAGGTGG AGTGGAACGG CTCTTGGAGT GATGGTTGGA AGGACTGGAG CTTTGTGGAC 
AAAGACGAGA AGGCCCGTCT GCAGCACCAG GTCACCGAGG ATGGAGAGTT CTGGATGTCA 
TATGATGACT TTGTCTACCA TTTCACAAAG CTGGAGATCT GCAACCTCAC AGCTGATGCC 
CTGGAGTCCG ATAAGCTTCA GACCTGGACA GTGTCTGTAA ATGAGGGCCG CTGGGTGAGG 
GGCTGTTCTG CTGGAGGCTG CCGGAACTTC CCAGACACTT TCTGGACCAA CCCGCAGTAC 
CGTCTCAAGC TCCTGGAGGA GGATGATGAC CCTGATGACT CTGAGGTGAT TTGCAGCTTC 
CTCGTGGCTC TGATGCAGAA AAATCGGCGC AAGGACCGGA AGCTGGGGGC CAACCTCTTC 
ACCATTGGCT TCGCTATCTA CGAGGTTCCC AAAGAGATGC ACGGGAATAA GCAACACCTG 
CAGAAGGACT TCTTCTTGTA CAATGCCTCC AAGGCCAGGA GCAAAACCTA CATCAACATG 
CGGGAGGTGT CCCAGCGCTT CCGCCTGCCG CCCAGCGAGT ATGTCATTGT CCCCTCCACT 
TACGAGCCCC ATCAGGAGGG GGAATTCATC CTCCGGGTCT TCTCTGAAAA GAGGAATCTC 
TCTGAGGAAG CTGAGAATAC AATCTCTGTG GACCGGCCAG TGCCACGGCC TGGCCACACA 
GACCAGGAGA GTGAGGAGCA GCAGCAATTC CGGAACATCT TCAGGCAGAT TGCAGGCGAC 
GACATGGAGA TCTGTGCGGA TGAACTCAAG AATGTCCTTA ATACGGTGGT GAACAAACGT 
GAGCGACTCG CGCCAGACGT GGGGGATGGG CAGGGGCCCC AGCTGTCTGA AAGCAGCTCC 
TCACCCTTCT CCATCCCCCC AGACAAGGAC CTGAAGACAC AAGGGTTCAC TCTGGAGTCC 
TGCAGAAGCA TGATAGCTCT CATGGATACA GATGGCTCTG GGAGACTGAA TCTTCAAGAG 
TTCCATCACC TCTGGAAAAA GATCAAGGCC TGGCAGAAAA TCTTCAAACA CTATGACACT 
GACCATTCTG GTACCATCAA TAGCTATGAG ATGCGAAATG CAGTCAATGA TGCAGGCTTC 
CATCTCAACA GCCAACTCTA TGACATCATC ACCATGCGCT ACGCAGACAA ACACATGAAC 
ATCGACTTTG ACAGCTTCAT CTGCTGCTTC GTCAGGCTGG AAGGGATGTT CAGAGCTTTT 
CACGCATTTG ACAAGGATGG AGATGGCATC ATCAAACTGA ACGTACTTGA GTGGCTGCAG 
CTTACCATGT ATGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006234790.1
CDS	90..2447
Translation	MPYLLPGFFCDRVIRERDRRNGEGTVSQPLKFEGQDFVVLKQRCLAQKCLFEDRVFPAGTQALGSHELSQKAKMKAITWKRPKEICENPRFIIGGANRTDICQGDLGDCWFLAAIACLTLNERLLFRVIPHDQSFTENYAGIFHFQFWRYGDWVDVVIDDCLPTYNNQLVFTKSNHRNEFWSALLEKAYAKLHGSYEALKGGNTTEAMEDFTGGVTEFFEIKDAPSDMYKIMRKAIERGSLMGCSIDDGTNMTYGTSPSGLNMGELIARMVRNMDNSLLRDSDLDPRASDDRPSRTIVPVQYETRMACGLVKGHAYSVTGLEEALFKGEKVKLVRLRNPWGQVEWNGSWSDGWKDWSFVDKDEKARLQHQVTEDGEFWMSYDDFVYHFTKLEICNLTADALESDKLQTWTVSVNEGRWVRGCSAGGCRNFPDTFWTNPQYRLKLLEEDDDPDDSEVICSFLVALMQKNRRKDRKLGANLFTIGFAIYEVPKEMHGNKQHLQKDFFLYNASKARSKTYINMREVSQRFRLPPSEYVIVPSTYEPHQEGEFILRVFSEKRNLSEEAENTISVDRPVPRPGHTDQESEEQQQFRNIFRQIAGDDMEICADELKNVLNTVVNKRERLAPDVGDGQGPQLSESSSSPFSIPPDKDLKTQGFTLESCRSMIALMDTDGSGRLNLQEFHHLWKKIKAWQKIFKHYDTDHSGTINSYEMRNAVNDAGFHLNSQLYDIITMRYADKHMNIDFDSFICCFVRLEGMFRAFHAFDKDGDGIIKLNVLEWLQLTMYA

Target ORF information:

RefSeq Version	XM_006234728.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus calpain 3 (Capn3), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006234728.3

ORF Insert Sequence:

ATGCCCTACC TGCTGCCGGG ATTCTTCTGT GACAGAGTGA TCAGAGAAAG GGACAGGAGA 
AATGGAGAGG GCACCGTCTC ACAGCCTCTC AAGTTTGAGG GGCAGGATTT TGTCGTTCTC 
AAACAACGGT GTCTGGCTCA GAAGTGCCTC TTTGAAGATC GAGTCTTCCC AGCAGGTACA 
CAGGCCCTTG GCTCACATGA GCTGAGCCAG AAAGCCAAGA TGAAGGCCAT CACTTGGAAG 
AGGCCAAAGG AAATTTGTGA GAATCCCCGA TTTATCATTG GTGGAGCCAA CAGGACTGAC 
ATCTGCCAAG GAGATCTAGG GGACTGCTGG TTTCTTGCAG CCATTGCCTG TCTGACCCTG 
AATGAGCGAC TGCTTTTCCG AGTTATACCT CATGATCAAA GTTTCACTGA AAACTACGCA 
GGGATCTTCC ACTTCCAGTT CTGGCGCTAT GGAGACTGGG TAGATGTGGT TATTGACGAC 
TGTCTGCCGA CATACAACAA CCAGCTGGTC TTCACCAAAT CCAACCACCG CAATGAGTTC 
TGGAGTGCTC TACTGGAGAA AGCATATGCC AAGCTCCATG GTTCCTATGA AGCTCTGAAA 
GGTGGGAACA CCACAGAAGC CATGGAGGAC TTCACAGGAG GGGTGACAGA GTTTTTTGAG 
ATCAAGGATG CTCCGAGTGA CATGTACAAG ATCATGAGGA AAGCTATCGA GAGAGGCTCC 
CTCATGGGCT GCTCCATTGA TGATGGCACC AACATGACTT ATGGAACCTC TCCTTCTGGT 
CTGAACATGG GGGAATTGAT TGCGCGGATG GTGAGAAATA TGGATAACTC GCTGCTCAGA 
GACTCAGACC TGGACCCCAG GGCCTCAGAT GACAGACCGT CACGGACAAT TGTTCCGGTG 
CAGTATGAAA CAAGAATGGC CTGTGGACTG GTGAAAGGGC ACGCCTATTC AGTCACTGGG 
CTGGAGGAGG CCCTGTTCAA AGGCGAGAAG GTGAAGCTGG TGCGGCTGCG GAACCCCTGG 
GGCCAGGTGG AGTGGAACGG CTCTTGGAGT GATGGTTGGA AGGACTGGAG CTTTGTGGAC 
AAAGACGAGA AGGCCCGTCT GCAGCACCAG GTCACCGAGG ATGGAGAGTT CTGGATGTCA 
TATGATGACT TTGTCTACCA TTTCACAAAG CTGGAGATCT GCAACCTCAC AGCTGATGCC 
CTGGAGTCCG ATAAGCTTCA GACCTGGACA GTGTCTGTAA ATGAGGGCCG CTGGGTGAGG 
GGCTGTTCTG CTGGAGGCTG CCGGAACTTC CCAGACACTT TCTGGACCAA CCCGCAGTAC 
CGTCTCAAGC TCCTGGAGGA GGATGATGAC CCTGATGACT CTGAGGTGAT TTGCAGCTTC 
CTCGTGGCTC TGATGCAGAA AAATCGGCGC AAGGACCGGA AGCTGGGGGC CAACCTCTTC 
ACCATTGGCT TCGCTATCTA CGAGGTTCCC AAAGAGATGC ACGGGAATAA GCAACACCTG 
CAGAAGGACT TCTTCTTGTA CAATGCCTCC AAGGCCAGGA GCAAAACCTA CATCAACATG 
CGGGAGGTGT CCCAGCGCTT CCGCCTGCCG CCCAGCGAGT ATGTCATTGT CCCCTCCACT 
TACGAGCCCC ATCAGGAGGG GGAATTCATC CTCCGGGTCT TCTCTGAAAA GAGGAATCTC 
TCTGAGGAAG CTGAGAATAC AATCTCTGTG GACCGGCCAG TGCCACGGCC TGGCCACACA 
GACCAGGAGA GTGAGGAGCA GCAGCAATTC CGGAACATCT TCAGGCAGAT TGCAGGCGAC 
GACATGGAGA TCTGTGCGGA TGAACTCAAG AATGTCCTTA ATACGGTGGT GAACAAACGT 
GAGCGACTCG CGCCAGACGT GGGGGATGGG CAGGGGCCCC AGCTGTCTGA AAGCAGCTCC 
TCACCCTTCT CCATCCCCCC AGACAAGGAC CTGAAGACAC AAGGGTTCAC TCTGGAGTCC 
TGCAGAAGCA TGATAGCTCT CATGGATACA GATGGCTCTG GGAGACTGAA TCTTCAAGAG 
TTCCATCACC TCTGGAAAAA GATCAAGGCC TGGCAGAAAA TCTTCAAACA CTATGACACT 
GACCATTCTG GTACCATCAA TAGCTATGAG ATGCGAAATG CAGTCAATGA TGCAGGCTTC 
CATCTCAACA GCCAACTCTA TGACATCATC ACCATGCGCT ACGCAGACAA ACACATGAAC 
ATCGACTTTG ACAGCTTCAT CTGCTGCTTC GTCAGGCTGG AAGGGATGTT CAGAGCTTTT 
CACGCATTTG ACAAGGATGG AGATGGCATC ATCAAACTGA ACGTACTTGA GTGGCTGCAG 
CTTACCATGT ATGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Capn3 ( XM_006234729.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006234791.1 Rattus norvegicus calpain 3 (Capn3), transcript variant X5, mRNA.

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CloneID	ORa26202
Clone ID Related Accession (Same CDS sequence)	XM_006234729.3
Accession Version	XM_006234729.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2274bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	calpain-3 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006234729.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCTACC TGCTGCCGGG ATTCTTCTGT GACAGAGTGA TCAGAGAAAG GGACAGGAGA 
AATGGAGAGG GCACCGTCTC ACAGCCTCTC AAGTTTGAGG GGCAGGATTT TGTCGTTCTC 
AAACAACGGT GTCTGGCTCA GAAGTGCCTC TTTGAAGATC GAGTCTTCCC AGCAGGTACA 
CAGGCCCTTG GCTCACATGA GCTGAGCCAG AAAGCCAAGA TGAAGGCCAT CACTTGGAAG 
AGGCCAAAGG AAATTTGTGA GAATCCCCGA TTTATCATTG GTGGAGCCAA CAGGACTGAC 
ATCTGCCAAG GAGATCTAGG GGACTGCTGG TTTCTTGCAG CCATTGCCTG TCTGACCCTG 
AATGAGCGAC TGCTTTTCCG AGTTATACCT CATGATCAAA GTTTCACTGA AAACTACGCA 
GGGATCTTCC ACTTCCAGTT CTGGCGCTAT GGAGACTGGG TAGATGTGGT TATTGACGAC 
TGTCTGCCGA CATACAACAA CCAGCTGGTC TTCACCAAAT CCAACCACCG CAATGAGTTC 
TGGAGTGCTC TACTGGAGAA AGCATATGCC AAGCTCCATG GTTCCTATGA AGCTCTGAAA 
GGTGGGAACA CCACAGAAGC CATGGAGGAC TTCACAGGAG GGGTGACAGA GTTTTTTGAG 
ATCAAGGATG CTCCGAGTGA CATGTACAAG ATCATGAGGA AAGCTATCGA GAGAGGCTCC 
CTCATGGGCT GCTCCATTGA TGATGGCACC AACATGACTT ATGGAACCTC TCCTTCTGGT 
CTGAACATGG GGGAATTGAT TGCGCGGATG GTGAGAAATA TGGATAACTC GCTGCTCAGA 
GACTCAGACC TGGACCCCAG GGCCTCAGAT GACAGACCGT CACGGACAAT TGTTCCGGTG 
CAGTATGAAA CAAGAATGGC CTGTGGACTG GTGAAAGGGC ACGCCTATTC AGTCACTGGG 
CTGGAGGAGG CCCTGTTCAA AGGCGAGAAG GTGAAGCTGG TGCGGCTGCG GAACCCCTGG 
GGCCAGGTGG AGTGGAACGG CTCTTGGAGT GATGGTTGGA AGGACTGGAG CTTTGTGGAC 
AAAGACGAGA AGGCCCGTCT GCAGCACCAG GTCACCGAGG ATGGAGAGTT CTGGATGTCA 
TATGATGACT TTGTCTACCA TTTCACAAAG CTGGAGATCT GCAACCTCAC AGCTGATGCC 
CTGGAGTCCG ATAAGCTTCA GACCTGGACA GTGTCTGTAA ATGAGGGCCG CTGGGTGAGG 
GGCTGTTCTG CTGGAGGCTG CCGGAACTTC CCAGACACTT TCTGGACCAA CCCGCAGTAC 
CGTCTCAAGC TCCTGGAGGA GGATGATGAC CCTGATGACT CTGAGGTGAT TTGCAGCTTC 
CTCGTGGCTC TGATGCAGAA AAATCGGCGC AAGGACCGGA AGCTGGGGGC CAACCTCTTC 
ACCATTGGCT TCGCTATCTA CGAGGTTCCC AAAGAGATGC ACGGGAATAA GCAACACCTG 
CAGAAGGACT TCTTCTTGTA CAATGCCTCC AAGGCCAGGA GCAAAACCTA CATCAACATG 
CGGGAGGTGT CCCAGCGCTT CCGCCTGCCG CCCAGCGAGT ATGTCATTGT CCCCTCCACT 
TACGAGCCCC ATCAGGAGGG GGAATTCATC CTCCGGGTCT TCTCTGAAAA GAGGAATCTC 
TCTGAGGAAG CTGAGAATAC AATCTCTGTG GACCGGCCAG TGCCACGGCC TGGCCACACA 
GACCAGGAGA GTGAGGAGCA GCAGCAATTC CGGAACATCT TCAGGCAGAT TGCAGGCGAC 
GACATGGAGA TCTGTGCGGA TGAACTCAAG AATGTCCTTA ATACGGTGGT GAACAAACAC 
AAGGACCTGA AGACACAAGG GTTCACTCTG GAGTCCTGCA GAAGCATGAT AGCTCTCATG 
GATACAGATG GCTCTGGGAG ACTGAATCTT CAAGAGTTCC ATCACCTCTG GAAAAAGATC 
AAGGCCTGGC AGAAAATCTT CAAACACTAT GACACTGACC ATTCTGGTAC CATCAATAGC 
TATGAGATGC GAAATGCAGT CAATGATGCA GGCTTCCATC TCAACAGCCA ACTCTATGAC 
ATCATCACCA TGCGCTACGC AGACAAACAC ATGAACATCG ACTTTGACAG CTTCATCTGC 
TGCTTCGTCA GGCTGGAAGG GATGTTCAGA GCTTTTCACG CATTTGACAA GGATGGAGAT 
GGCATCATCA AACTGAACGT ACTTGAGTGG CTGCAGCTTA CCATGTATGC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006234791.1
CDS	86..2359
Translation	MPYLLPGFFCDRVIRERDRRNGEGTVSQPLKFEGQDFVVLKQRCLAQKCLFEDRVFPAGTQALGSHELSQKAKMKAITWKRPKEICENPRFIIGGANRTDICQGDLGDCWFLAAIACLTLNERLLFRVIPHDQSFTENYAGIFHFQFWRYGDWVDVVIDDCLPTYNNQLVFTKSNHRNEFWSALLEKAYAKLHGSYEALKGGNTTEAMEDFTGGVTEFFEIKDAPSDMYKIMRKAIERGSLMGCSIDDGTNMTYGTSPSGLNMGELIARMVRNMDNSLLRDSDLDPRASDDRPSRTIVPVQYETRMACGLVKGHAYSVTGLEEALFKGEKVKLVRLRNPWGQVEWNGSWSDGWKDWSFVDKDEKARLQHQVTEDGEFWMSYDDFVYHFTKLEICNLTADALESDKLQTWTVSVNEGRWVRGCSAGGCRNFPDTFWTNPQYRLKLLEEDDDPDDSEVICSFLVALMQKNRRKDRKLGANLFTIGFAIYEVPKEMHGNKQHLQKDFFLYNASKARSKTYINMREVSQRFRLPPSEYVIVPSTYEPHQEGEFILRVFSEKRNLSEEAENTISVDRPVPRPGHTDQESEEQQQFRNIFRQIAGDDMEICADELKNVLNTVVNKHKDLKTQGFTLESCRSMIALMDTDGSGRLNLQEFHHLWKKIKAWQKIFKHYDTDHSGTINSYEMRNAVNDAGFHLNSQLYDIITMRYADKHMNIDFDSFICCFVRLEGMFRAFHAFDKDGDGIIKLNVLEWLQLTMYA

Target ORF information:

RefSeq Version	XM_006234729.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus calpain 3 (Capn3), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006234729.3

ORF Insert Sequence:

ATGCCCTACC TGCTGCCGGG ATTCTTCTGT GACAGAGTGA TCAGAGAAAG GGACAGGAGA 
AATGGAGAGG GCACCGTCTC ACAGCCTCTC AAGTTTGAGG GGCAGGATTT TGTCGTTCTC 
AAACAACGGT GTCTGGCTCA GAAGTGCCTC TTTGAAGATC GAGTCTTCCC AGCAGGTACA 
CAGGCCCTTG GCTCACATGA GCTGAGCCAG AAAGCCAAGA TGAAGGCCAT CACTTGGAAG 
AGGCCAAAGG AAATTTGTGA GAATCCCCGA TTTATCATTG GTGGAGCCAA CAGGACTGAC 
ATCTGCCAAG GAGATCTAGG GGACTGCTGG TTTCTTGCAG CCATTGCCTG TCTGACCCTG 
AATGAGCGAC TGCTTTTCCG AGTTATACCT CATGATCAAA GTTTCACTGA AAACTACGCA 
GGGATCTTCC ACTTCCAGTT CTGGCGCTAT GGAGACTGGG TAGATGTGGT TATTGACGAC 
TGTCTGCCGA CATACAACAA CCAGCTGGTC TTCACCAAAT CCAACCACCG CAATGAGTTC 
TGGAGTGCTC TACTGGAGAA AGCATATGCC AAGCTCCATG GTTCCTATGA AGCTCTGAAA 
GGTGGGAACA CCACAGAAGC CATGGAGGAC TTCACAGGAG GGGTGACAGA GTTTTTTGAG 
ATCAAGGATG CTCCGAGTGA CATGTACAAG ATCATGAGGA AAGCTATCGA GAGAGGCTCC 
CTCATGGGCT GCTCCATTGA TGATGGCACC AACATGACTT ATGGAACCTC TCCTTCTGGT 
CTGAACATGG GGGAATTGAT TGCGCGGATG GTGAGAAATA TGGATAACTC GCTGCTCAGA 
GACTCAGACC TGGACCCCAG GGCCTCAGAT GACAGACCGT CACGGACAAT TGTTCCGGTG 
CAGTATGAAA CAAGAATGGC CTGTGGACTG GTGAAAGGGC ACGCCTATTC AGTCACTGGG 
CTGGAGGAGG CCCTGTTCAA AGGCGAGAAG GTGAAGCTGG TGCGGCTGCG GAACCCCTGG 
GGCCAGGTGG AGTGGAACGG CTCTTGGAGT GATGGTTGGA AGGACTGGAG CTTTGTGGAC 
AAAGACGAGA AGGCCCGTCT GCAGCACCAG GTCACCGAGG ATGGAGAGTT CTGGATGTCA 
TATGATGACT TTGTCTACCA TTTCACAAAG CTGGAGATCT GCAACCTCAC AGCTGATGCC 
CTGGAGTCCG ATAAGCTTCA GACCTGGACA GTGTCTGTAA ATGAGGGCCG CTGGGTGAGG 
GGCTGTTCTG CTGGAGGCTG CCGGAACTTC CCAGACACTT TCTGGACCAA CCCGCAGTAC 
CGTCTCAAGC TCCTGGAGGA GGATGATGAC CCTGATGACT CTGAGGTGAT TTGCAGCTTC 
CTCGTGGCTC TGATGCAGAA AAATCGGCGC AAGGACCGGA AGCTGGGGGC CAACCTCTTC 
ACCATTGGCT TCGCTATCTA CGAGGTTCCC AAAGAGATGC ACGGGAATAA GCAACACCTG 
CAGAAGGACT TCTTCTTGTA CAATGCCTCC AAGGCCAGGA GCAAAACCTA CATCAACATG 
CGGGAGGTGT CCCAGCGCTT CCGCCTGCCG CCCAGCGAGT ATGTCATTGT CCCCTCCACT 
TACGAGCCCC ATCAGGAGGG GGAATTCATC CTCCGGGTCT TCTCTGAAAA GAGGAATCTC 
TCTGAGGAAG CTGAGAATAC AATCTCTGTG GACCGGCCAG TGCCACGGCC TGGCCACACA 
GACCAGGAGA GTGAGGAGCA GCAGCAATTC CGGAACATCT TCAGGCAGAT TGCAGGCGAC 
GACATGGAGA TCTGTGCGGA TGAACTCAAG AATGTCCTTA ATACGGTGGT GAACAAACAC 
AAGGACCTGA AGACACAAGG GTTCACTCTG GAGTCCTGCA GAAGCATGAT AGCTCTCATG 
GATACAGATG GCTCTGGGAG ACTGAATCTT CAAGAGTTCC ATCACCTCTG GAAAAAGATC 
AAGGCCTGGC AGAAAATCTT CAAACACTAT GACACTGACC ATTCTGGTAC CATCAATAGC 
TATGAGATGC GAAATGCAGT CAATGATGCA GGCTTCCATC TCAACAGCCA ACTCTATGAC 
ATCATCACCA TGCGCTACGC AGACAAACAC ATGAACATCG ACTTTGACAG CTTCATCTGC 
TGCTTCGTCA GGCTGGAAGG GATGTTCAGA GCTTTTCACG CATTTGACAA GGATGGAGAT 
GGCATCATCA AACTGAACGT ACTTGAGTGG CTGCAGCTTA CCATGTATGC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Capn3 ( XM_008762080.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008760302.1 Rattus norvegicus calpain 3 (Capn3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa43223
Clone ID Related Accession (Same CDS sequence)	XM_008762080.2
Accession Version	XM_008762080.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2418bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	calpain-3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008762080.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGACCG TTATTAGTCC AACTGTGGCC CCAAGGACAG GAGCTGAGCC CAGGTCCCCA 
GGGCCAGTTC CTCACCCAGC TCAAGGCAAG ACCACTGAGG CTGGAGGTGG ACACCCGGGT 
GGCATCTATT CAGCCATCAT CAGCCGCAAT TTTCCGATCA TTGGTGTGAA AGAGAAGACA 
TTCGAGCAGC TCCACAAGAA GTGCCTAGAG AAGAAAGTTC TTTACCTGGA TCCCGAGTTC 
CCACCGGATG AGACCTCTCT CTTTTACAGC CAGAAGTTCC CCATCCAGTT CGTCTGGAAG 
AGACCTCCGG AAATTTGTGA GAATCCCCGA TTTATCATTG GTGGAGCCAA CAGGACTGAC 
ATCTGCCAAG GAGATCTAGG GGACTGCTGG TTTCTTGCAG CCATTGCCTG TCTGACCCTG 
AATGAGCGAC TGCTTTTCCG AGTTATACCT CATGATCAAA GTTTCACTGA AAACTACGCA 
GGGATCTTCC ACTTCCAGTT CTGGCGCTAT GGAGACTGGG TAGATGTGGT TATTGACGAC 
TGTCTGCCGA CATACAACAA CCAGCTGGTC TTCACCAAAT CCAACCACCG CAATGAGTTC 
TGGAGTGCTC TACTGGAGAA AGCATATGCC AAGCTCCATG GTTCCTATGA AGCTCTGAAA 
GGTGGGAACA CCACAGAAGC CATGGAGGAC TTCACAGGAG GGGTGACAGA GTTTTTTGAG 
ATCAAGGATG CTCCGAGTGA CATGTACAAG ATCATGAGGA AAGCTATCGA GAGAGGCTCC 
CTCATGGGCT GCTCCATTGA TGATGGCACC AACATGACTT ATGGAACCTC TCCTTCTGGT 
CTGAACATGG GGGAATTGAT TGCGCGGATG GTGAGAAATA TGGATAACTC GCTGCTCAGA 
GACTCAGACC TGGACCCCAG GGCCTCAGAT GACAGACCGT CACGGACAAT TGTTCCGGTG 
CAGTATGAAA CAAGAATGGC CTGTGGACTG GTGAAAGGGC ACGCCTATTC AGTCACTGGG 
CTGGAGGAGG CCCTGTTCAA AGGCGAGAAG GTGAAGCTGG TGCGGCTGCG GAACCCCTGG 
GGCCAGGTGG AGTGGAACGG CTCTTGGAGT GATGGTTGGA AGGACTGGAG CTTTGTGGAC 
AAAGACGAGA AGGCCCGTCT GCAGCACCAG GTCACCGAGG ATGGAGAGTT CTGGATGTCA 
TATGATGACT TTGTCTACCA TTTCACAAAG CTGGAGATCT GCAACCTCAC AGCTGATGCC 
CTGGAGTCCG ATAAGCTTCA GACCTGGACA GTGTCTGTAA ATGAGGGCCG CTGGGTGAGG 
GGCTGTTCTG CTGGAGGCTG CCGGAACTTC CCAGACACTT TCTGGACCAA CCCGCAGTAC 
CGTCTCAAGC TCCTGGAGGA GGATGATGAC CCTGATGACT CTGAGGTGAT TTGCAGCTTC 
CTCGTGGCTC TGATGCAGAA AAATCGGCGC AAGGACCGGA AGCTGGGGGC CAACCTCTTC 
ACCATTGGCT TCGCTATCTA CGAGGTTCCC AAAGAGATGC ACGGGAATAA GCAACACCTG 
CAGAAGGACT TCTTCTTGTA CAATGCCTCC AAGGCCAGGA GCAAAACCTA CATCAACATG 
CGGGAGGTGT CCCAGCGCTT CCGCCTGCCG CCCAGCGAGT ATGTCATTGT CCCCTCCACT 
TACGAGCCCC ATCAGGAGGG GGAATTCATC CTCCGGGTCT TCTCTGAAAA GAGGAATCTC 
TCTGAGGAAG CTGAGAATAC AATCTCTGTG GACCGGCCAG TGCCACGGCC TGGCCACACA 
GACCAGGAGA GTGAGGAGCA GCAGCAATTC CGGAACATCT TCAGGCAGAT TGCAGGCGAC 
GACATGGAGA TCTGTGCGGA TGAACTCAAG AATGTCCTTA ATACGGTGGT GAACAAACGT 
GAGCGACTCG CGCCAGACGT GGGGGATGGG CAGGGGCCCC AGCTGTCTGA AAGCAGCTCC 
TCACCCTTCT CCATCCCCCC AGACAAGGAC CTGAAGACAC AAGGGTTCAC TCTGGAGTCC 
TGCAGAAGCA TGATAGCTCT CATGGATACA GATGGCTCTG GGAGACTGAA TCTTCAAGAG 
TTCCATCACC TCTGGAAAAA GATCAAGGCC TGGCAGAAAA TCTTCAAACA CTATGACACT 
GACCATTCTG GTACCATCAA TAGCTATGAG ATGCGAAATG CAGTCAATGA TGCAGGCTTC 
CATCTCAACA GCCAACTCTA TGACATCATC ACCATGCGCT ACGCAGACAA ACACATGAAC 
ATCGACTTTG ACAGCTTCAT CTGCTGCTTC GTCAGGCTGG AAGGGATGTT CAGAGCTTTT 
CACGCATTTG ACAAGGATGG AGATGGCATC ATCAAACTGA ACGTACTTGA GTGGCTGCAG 
CTTACCATGT ATGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008760302.1
CDS	1434..3851
Translation	MPTVISPTVAPRTGAEPRSPGPVPHPAQGKTTEAGGGHPGGIYSAIISRNFPIIGVKEKTFEQLHKKCLEKKVLYLDPEFPPDETSLFYSQKFPIQFVWKRPPEICENPRFIIGGANRTDICQGDLGDCWFLAAIACLTLNERLLFRVIPHDQSFTENYAGIFHFQFWRYGDWVDVVIDDCLPTYNNQLVFTKSNHRNEFWSALLEKAYAKLHGSYEALKGGNTTEAMEDFTGGVTEFFEIKDAPSDMYKIMRKAIERGSLMGCSIDDGTNMTYGTSPSGLNMGELIARMVRNMDNSLLRDSDLDPRASDDRPSRTIVPVQYETRMACGLVKGHAYSVTGLEEALFKGEKVKLVRLRNPWGQVEWNGSWSDGWKDWSFVDKDEKARLQHQVTEDGEFWMSYDDFVYHFTKLEICNLTADALESDKLQTWTVSVNEGRWVRGCSAGGCRNFPDTFWTNPQYRLKLLEEDDDPDDSEVICSFLVALMQKNRRKDRKLGANLFTIGFAIYEVPKEMHGNKQHLQKDFFLYNASKARSKTYINMREVSQRFRLPPSEYVIVPSTYEPHQEGEFILRVFSEKRNLSEEAENTISVDRPVPRPGHTDQESEEQQQFRNIFRQIAGDDMEICADELKNVLNTVVNKRERLAPDVGDGQGPQLSESSSSPFSIPPDKDLKTQGFTLESCRSMIALMDTDGSGRLNLQEFHHLWKKIKAWQKIFKHYDTDHSGTINSYEMRNAVNDAGFHLNSQLYDIITMRYADKHMNIDFDSFICCFVRLEGMFRAFHAFDKDGDGIIKLNVLEWLQLTMYA

Target ORF information:

RefSeq Version	XM_008762080.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus calpain 3 (Capn3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008762080.2

ORF Insert Sequence:

ATGCCGACCG TTATTAGTCC AACTGTGGCC CCAAGGACAG GAGCTGAGCC CAGGTCCCCA 
GGGCCAGTTC CTCACCCAGC TCAAGGCAAG ACCACTGAGG CTGGAGGTGG ACACCCGGGT 
GGCATCTATT CAGCCATCAT CAGCCGCAAT TTTCCGATCA TTGGTGTGAA AGAGAAGACA 
TTCGAGCAGC TCCACAAGAA GTGCCTAGAG AAGAAAGTTC TTTACCTGGA TCCCGAGTTC 
CCACCGGATG AGACCTCTCT CTTTTACAGC CAGAAGTTCC CCATCCAGTT CGTCTGGAAG 
AGACCTCCGG AAATTTGTGA GAATCCCCGA TTTATCATTG GTGGAGCCAA CAGGACTGAC 
ATCTGCCAAG GAGATCTAGG GGACTGCTGG TTTCTTGCAG CCATTGCCTG TCTGACCCTG 
AATGAGCGAC TGCTTTTCCG AGTTATACCT CATGATCAAA GTTTCACTGA AAACTACGCA 
GGGATCTTCC ACTTCCAGTT CTGGCGCTAT GGAGACTGGG TAGATGTGGT TATTGACGAC 
TGTCTGCCGA CATACAACAA CCAGCTGGTC TTCACCAAAT CCAACCACCG CAATGAGTTC 
TGGAGTGCTC TACTGGAGAA AGCATATGCC AAGCTCCATG GTTCCTATGA AGCTCTGAAA 
GGTGGGAACA CCACAGAAGC CATGGAGGAC TTCACAGGAG GGGTGACAGA GTTTTTTGAG 
ATCAAGGATG CTCCGAGTGA CATGTACAAG ATCATGAGGA AAGCTATCGA GAGAGGCTCC 
CTCATGGGCT GCTCCATTGA TGATGGCACC AACATGACTT ATGGAACCTC TCCTTCTGGT 
CTGAACATGG GGGAATTGAT TGCGCGGATG GTGAGAAATA TGGATAACTC GCTGCTCAGA 
GACTCAGACC TGGACCCCAG GGCCTCAGAT GACAGACCGT CACGGACAAT TGTTCCGGTG 
CAGTATGAAA CAAGAATGGC CTGTGGACTG GTGAAAGGGC ACGCCTATTC AGTCACTGGG 
CTGGAGGAGG CCCTGTTCAA AGGCGAGAAG GTGAAGCTGG TGCGGCTGCG GAACCCCTGG 
GGCCAGGTGG AGTGGAACGG CTCTTGGAGT GATGGTTGGA AGGACTGGAG CTTTGTGGAC 
AAAGACGAGA AGGCCCGTCT GCAGCACCAG GTCACCGAGG ATGGAGAGTT CTGGATGTCA 
TATGATGACT TTGTCTACCA TTTCACAAAG CTGGAGATCT GCAACCTCAC AGCTGATGCC 
CTGGAGTCCG ATAAGCTTCA GACCTGGACA GTGTCTGTAA ATGAGGGCCG CTGGGTGAGG 
GGCTGTTCTG CTGGAGGCTG CCGGAACTTC CCAGACACTT TCTGGACCAA CCCGCAGTAC 
CGTCTCAAGC TCCTGGAGGA GGATGATGAC CCTGATGACT CTGAGGTGAT TTGCAGCTTC 
CTCGTGGCTC TGATGCAGAA AAATCGGCGC AAGGACCGGA AGCTGGGGGC CAACCTCTTC 
ACCATTGGCT TCGCTATCTA CGAGGTTCCC AAAGAGATGC ACGGGAATAA GCAACACCTG 
CAGAAGGACT TCTTCTTGTA CAATGCCTCC AAGGCCAGGA GCAAAACCTA CATCAACATG 
CGGGAGGTGT CCCAGCGCTT CCGCCTGCCG CCCAGCGAGT ATGTCATTGT CCCCTCCACT 
TACGAGCCCC ATCAGGAGGG GGAATTCATC CTCCGGGTCT TCTCTGAAAA GAGGAATCTC 
TCTGAGGAAG CTGAGAATAC AATCTCTGTG GACCGGCCAG TGCCACGGCC TGGCCACACA 
GACCAGGAGA GTGAGGAGCA GCAGCAATTC CGGAACATCT TCAGGCAGAT TGCAGGCGAC 
GACATGGAGA TCTGTGCGGA TGAACTCAAG AATGTCCTTA ATACGGTGGT GAACAAACGT 
GAGCGACTCG CGCCAGACGT GGGGGATGGG CAGGGGCCCC AGCTGTCTGA AAGCAGCTCC 
TCACCCTTCT CCATCCCCCC AGACAAGGAC CTGAAGACAC AAGGGTTCAC TCTGGAGTCC 
TGCAGAAGCA TGATAGCTCT CATGGATACA GATGGCTCTG GGAGACTGAA TCTTCAAGAG 
TTCCATCACC TCTGGAAAAA GATCAAGGCC TGGCAGAAAA TCTTCAAACA CTATGACACT 
GACCATTCTG GTACCATCAA TAGCTATGAG ATGCGAAATG CAGTCAATGA TGCAGGCTTC 
CATCTCAACA GCCAACTCTA TGACATCATC ACCATGCGCT ACGCAGACAA ACACATGAAC 
ATCGACTTTG ACAGCTTCAT CTGCTGCTTC GTCAGGCTGG AAGGGATGTT CAGAGCTTTT 
CACGCATTTG ACAAGGATGG AGATGGCATC ATCAAACTGA ACGTACTTGA GTGGCTGCAG 
CTTACCATGT ATGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Capn3 ( XM_008762081.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008760303.1 Rattus norvegicus calpain 3 (Capn3), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ORa43224
Clone ID Related Accession (Same CDS sequence)	XM_008762081.2
Accession Version	XM_008762081.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2334bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	calpain-3 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008762081.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGACCG TTATTAGTCC AACTGTGGCC CCAAGGACAG GAGCTGAGCC CAGGTCCCCA 
GGGCCAGTTC CTCACCCAGC TCAAGGCAAG ACCACTGAGG CTGGAGGTGG ACACCCGGGT 
GGCATCTATT CAGCCATCAT CAGCCGCAAT TTTCCGATCA TTGGTGTGAA AGAGAAGACA 
TTCGAGCAGC TCCACAAGAA GTGCCTAGAG AAGAAAGTTC TTTACCTGGA TCCCGAGTTC 
CCACCGGATG AGACCTCTCT CTTTTACAGC CAGAAGTTCC CCATCCAGTT CGTCTGGAAG 
AGACCTCCGG AAATTTGTGA GAATCCCCGA TTTATCATTG GTGGAGCCAA CAGGACTGAC 
ATCTGCCAAG GAGATCTAGG GGACTGCTGG TTTCTTGCAG CCATTGCCTG TCTGACCCTG 
AATGAGCGAC TGCTTTTCCG AGTTATACCT CATGATCAAA GTTTCACTGA AAACTACGCA 
GGGATCTTCC ACTTCCAGTT CTGGCGCTAT GGAGACTGGG TAGATGTGGT TATTGACGAC 
TGTCTGCCGA CATACAACAA CCAGCTGGTC TTCACCAAAT CCAACCACCG CAATGAGTTC 
TGGAGTGCTC TACTGGAGAA AGCATATGCC AAGCTCCATG GTTCCTATGA AGCTCTGAAA 
GGTGGGAACA CCACAGAAGC CATGGAGGAC TTCACAGGAG GGGTGACAGA GTTTTTTGAG 
ATCAAGGATG CTCCGAGTGA CATGTACAAG ATCATGAGGA AAGCTATCGA GAGAGGCTCC 
CTCATGGGCT GCTCCATTGA TGATGGCACC AACATGACTT ATGGAACCTC TCCTTCTGGT 
CTGAACATGG GGGAATTGAT TGCGCGGATG GTGAGAAATA TGGATAACTC GCTGCTCAGA 
GACTCAGACC TGGACCCCAG GGCCTCAGAT GACAGACCGT CACGGACAAT TGTTCCGGTG 
CAGTATGAAA CAAGAATGGC CTGTGGACTG GTGAAAGGGC ACGCCTATTC AGTCACTGGG 
CTGGAGGAGG CCCTGTTCAA AGGCGAGAAG GTGAAGCTGG TGCGGCTGCG GAACCCCTGG 
GGCCAGGTGG AGTGGAACGG CTCTTGGAGT GATGGTTGGA AGGACTGGAG CTTTGTGGAC 
AAAGACGAGA AGGCCCGTCT GCAGCACCAG GTCACCGAGG ATGGAGAGTT CTGGATGTCA 
TATGATGACT TTGTCTACCA TTTCACAAAG CTGGAGATCT GCAACCTCAC AGCTGATGCC 
CTGGAGTCCG ATAAGCTTCA GACCTGGACA GTGTCTGTAA ATGAGGGCCG CTGGGTGAGG 
GGCTGTTCTG CTGGAGGCTG CCGGAACTTC CCAGACACTT TCTGGACCAA CCCGCAGTAC 
CGTCTCAAGC TCCTGGAGGA GGATGATGAC CCTGATGACT CTGAGGTGAT TTGCAGCTTC 
CTCGTGGCTC TGATGCAGAA AAATCGGCGC AAGGACCGGA AGCTGGGGGC CAACCTCTTC 
ACCATTGGCT TCGCTATCTA CGAGGTTCCC AAAGAGATGC ACGGGAATAA GCAACACCTG 
CAGAAGGACT TCTTCTTGTA CAATGCCTCC AAGGCCAGGA GCAAAACCTA CATCAACATG 
CGGGAGGTGT CCCAGCGCTT CCGCCTGCCG CCCAGCGAGT ATGTCATTGT CCCCTCCACT 
TACGAGCCCC ATCAGGAGGG GGAATTCATC CTCCGGGTCT TCTCTGAAAA GAGGAATCTC 
TCTGAGGAAG CTGAGAATAC AATCTCTGTG GACCGGCCAG TGCCACGGCC TGGCCACACA 
GACCAGGAGA GTGAGGAGCA GCAGCAATTC CGGAACATCT TCAGGCAGAT TGCAGGCGAC 
GACATGGAGA TCTGTGCGGA TGAACTCAAG AATGTCCTTA ATACGGTGGT GAACAAACAC 
AAGGACCTGA AGACACAAGG GTTCACTCTG GAGTCCTGCA GAAGCATGAT AGCTCTCATG 
GATACAGATG GCTCTGGGAG ACTGAATCTT CAAGAGTTCC ATCACCTCTG GAAAAAGATC 
AAGGCCTGGC AGAAAATCTT CAAACACTAT GACACTGACC ATTCTGGTAC CATCAATAGC 
TATGAGATGC GAAATGCAGT CAATGATGCA GGCTTCCATC TCAACAGCCA ACTCTATGAC 
ATCATCACCA TGCGCTACGC AGACAAACAC ATGAACATCG ACTTTGACAG CTTCATCTGC 
TGCTTCGTCA GGCTGGAAGG GATGTTCAGA GCTTTTCACG CATTTGACAA GGATGGAGAT 
GGCATCATCA AACTGAACGT ACTTGAGTGG CTGCAGCTTA CCATGTATGC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008760303.1
CDS	1433..3766
Translation	MPTVISPTVAPRTGAEPRSPGPVPHPAQGKTTEAGGGHPGGIYSAIISRNFPIIGVKEKTFEQLHKKCLEKKVLYLDPEFPPDETSLFYSQKFPIQFVWKRPPEICENPRFIIGGANRTDICQGDLGDCWFLAAIACLTLNERLLFRVIPHDQSFTENYAGIFHFQFWRYGDWVDVVIDDCLPTYNNQLVFTKSNHRNEFWSALLEKAYAKLHGSYEALKGGNTTEAMEDFTGGVTEFFEIKDAPSDMYKIMRKAIERGSLMGCSIDDGTNMTYGTSPSGLNMGELIARMVRNMDNSLLRDSDLDPRASDDRPSRTIVPVQYETRMACGLVKGHAYSVTGLEEALFKGEKVKLVRLRNPWGQVEWNGSWSDGWKDWSFVDKDEKARLQHQVTEDGEFWMSYDDFVYHFTKLEICNLTADALESDKLQTWTVSVNEGRWVRGCSAGGCRNFPDTFWTNPQYRLKLLEEDDDPDDSEVICSFLVALMQKNRRKDRKLGANLFTIGFAIYEVPKEMHGNKQHLQKDFFLYNASKARSKTYINMREVSQRFRLPPSEYVIVPSTYEPHQEGEFILRVFSEKRNLSEEAENTISVDRPVPRPGHTDQESEEQQQFRNIFRQIAGDDMEICADELKNVLNTVVNKHKDLKTQGFTLESCRSMIALMDTDGSGRLNLQEFHHLWKKIKAWQKIFKHYDTDHSGTINSYEMRNAVNDAGFHLNSQLYDIITMRYADKHMNIDFDSFICCFVRLEGMFRAFHAFDKDGDGIIKLNVLEWLQLTMYA

Target ORF information:

RefSeq Version	XM_008762081.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus calpain 3 (Capn3), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008762081.2

ORF Insert Sequence:

ATGCCGACCG TTATTAGTCC AACTGTGGCC CCAAGGACAG GAGCTGAGCC CAGGTCCCCA 
GGGCCAGTTC CTCACCCAGC TCAAGGCAAG ACCACTGAGG CTGGAGGTGG ACACCCGGGT 
GGCATCTATT CAGCCATCAT CAGCCGCAAT TTTCCGATCA TTGGTGTGAA AGAGAAGACA 
TTCGAGCAGC TCCACAAGAA GTGCCTAGAG AAGAAAGTTC TTTACCTGGA TCCCGAGTTC 
CCACCGGATG AGACCTCTCT CTTTTACAGC CAGAAGTTCC CCATCCAGTT CGTCTGGAAG 
AGACCTCCGG AAATTTGTGA GAATCCCCGA TTTATCATTG GTGGAGCCAA CAGGACTGAC 
ATCTGCCAAG GAGATCTAGG GGACTGCTGG TTTCTTGCAG CCATTGCCTG TCTGACCCTG 
AATGAGCGAC TGCTTTTCCG AGTTATACCT CATGATCAAA GTTTCACTGA AAACTACGCA 
GGGATCTTCC ACTTCCAGTT CTGGCGCTAT GGAGACTGGG TAGATGTGGT TATTGACGAC 
TGTCTGCCGA CATACAACAA CCAGCTGGTC TTCACCAAAT CCAACCACCG CAATGAGTTC 
TGGAGTGCTC TACTGGAGAA AGCATATGCC AAGCTCCATG GTTCCTATGA AGCTCTGAAA 
GGTGGGAACA CCACAGAAGC CATGGAGGAC TTCACAGGAG GGGTGACAGA GTTTTTTGAG 
ATCAAGGATG CTCCGAGTGA CATGTACAAG ATCATGAGGA AAGCTATCGA GAGAGGCTCC 
CTCATGGGCT GCTCCATTGA TGATGGCACC AACATGACTT ATGGAACCTC TCCTTCTGGT 
CTGAACATGG GGGAATTGAT TGCGCGGATG GTGAGAAATA TGGATAACTC GCTGCTCAGA 
GACTCAGACC TGGACCCCAG GGCCTCAGAT GACAGACCGT CACGGACAAT TGTTCCGGTG 
CAGTATGAAA CAAGAATGGC CTGTGGACTG GTGAAAGGGC ACGCCTATTC AGTCACTGGG 
CTGGAGGAGG CCCTGTTCAA AGGCGAGAAG GTGAAGCTGG TGCGGCTGCG GAACCCCTGG 
GGCCAGGTGG AGTGGAACGG CTCTTGGAGT GATGGTTGGA AGGACTGGAG CTTTGTGGAC 
AAAGACGAGA AGGCCCGTCT GCAGCACCAG GTCACCGAGG ATGGAGAGTT CTGGATGTCA 
TATGATGACT TTGTCTACCA TTTCACAAAG CTGGAGATCT GCAACCTCAC AGCTGATGCC 
CTGGAGTCCG ATAAGCTTCA GACCTGGACA GTGTCTGTAA ATGAGGGCCG CTGGGTGAGG 
GGCTGTTCTG CTGGAGGCTG CCGGAACTTC CCAGACACTT TCTGGACCAA CCCGCAGTAC 
CGTCTCAAGC TCCTGGAGGA GGATGATGAC CCTGATGACT CTGAGGTGAT TTGCAGCTTC 
CTCGTGGCTC TGATGCAGAA AAATCGGCGC AAGGACCGGA AGCTGGGGGC CAACCTCTTC 
ACCATTGGCT TCGCTATCTA CGAGGTTCCC AAAGAGATGC ACGGGAATAA GCAACACCTG 
CAGAAGGACT TCTTCTTGTA CAATGCCTCC AAGGCCAGGA GCAAAACCTA CATCAACATG 
CGGGAGGTGT CCCAGCGCTT CCGCCTGCCG CCCAGCGAGT ATGTCATTGT CCCCTCCACT 
TACGAGCCCC ATCAGGAGGG GGAATTCATC CTCCGGGTCT TCTCTGAAAA GAGGAATCTC 
TCTGAGGAAG CTGAGAATAC AATCTCTGTG GACCGGCCAG TGCCACGGCC TGGCCACACA 
GACCAGGAGA GTGAGGAGCA GCAGCAATTC CGGAACATCT TCAGGCAGAT TGCAGGCGAC 
GACATGGAGA TCTGTGCGGA TGAACTCAAG AATGTCCTTA ATACGGTGGT GAACAAACAC 
AAGGACCTGA AGACACAAGG GTTCACTCTG GAGTCCTGCA GAAGCATGAT AGCTCTCATG 
GATACAGATG GCTCTGGGAG ACTGAATCTT CAAGAGTTCC ATCACCTCTG GAAAAAGATC 
AAGGCCTGGC AGAAAATCTT CAAACACTAT GACACTGACC ATTCTGGTAC CATCAATAGC 
TATGAGATGC GAAATGCAGT CAATGATGCA GGCTTCCATC TCAACAGCCA ACTCTATGAC 
ATCATCACCA TGCGCTACGC AGACAAACAC ATGAACATCG ACTTTGACAG CTTCATCTGC 
TGCTTCGTCA GGCTGGAAGG GATGTTCAGA GCTTTTCACG CATTTGACAA GGATGGAGAT 
GGCATCATCA AACTGAACGT ACTTGAGTGG CTGCAGCTTA CCATGTATGC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Capn3 ( XM_017591508.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017446997.1 Rattus norvegicus calpain 3 (Capn3), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa60285
Clone ID Related Accession (Same CDS sequence)	XM_017591508.1
Accession Version	XM_017591508.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2406bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	calpain-3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCTACC TGCTGCCGGG ATTCTTCTGT GACAGAGTGA TCAGAGAAAG GGACAGGAGA 
AATGGAGAGG GCACCGTCTC ACAGCCTCTC AAGTTTGAGG GGCAGGATTT TGTCGTTCTC 
AAACAACGGT GTCTGGCTCA GAAGTGCCTC TTTGAAGATC GAGTCTTCCC AGCAGGTACA 
CAGGCCCTTG GCTCACATGA GCTGAGCCAG AAAGCCAAGA TGAAGGCCAT CACTTGGAAG 
AGGCCAAAGG AAATTTGTGA GAATCCCCGA TTTATCATTG GTGGAGCCAA CAGGACTGAC 
ATCTGCCAAG GAGATCTAGG GGACTGCTGG TTTCTTGCAG CCATTGCCTG TCTGACCCTG 
AATGAGCGAC TGCTTTTCCG AGTTATACCT CATGATCAAA GTTTCACTGA AAACTACGCA 
GGGATCTTCC ACTTCCAGTT CTGGCGCTAT GGAGACTGGG TAGATGTGGT TATTGACGAC 
TGTCTGCCGA CATACAACAA CCAGCTGGTC TTCACCAAAT CCAACCACCG CAATGAGTTC 
TGGAGTGCTC TACTGGAGAA AGCATATGCC AAGCTCCATG GTTCCTATGA AGCTCTGAAA 
GGTGGGAACA CCACAGAAGC CATGGAGGAC TTCACAGGAG GGGTGACAGA GTTTTTTGAG 
ATCAAGGATG CTCCGAGTGA CATGTACAAG ATCATGAGGA AAGCTATCGA GAGAGGCTCC 
CTCATGGGCT GCTCCATTGA TGATGGCACC AACATGACTT ATGGAACCTC TCCTTCTGGT 
CTGAACATGG GGGAATTGAT TGCGCGGATG GTGAGAAATA TGGATAACTC GCTGCTCAGA 
GACTCAGACC TGGACCCCAG GGCCTCAGAT GACAGACCGT CACGGACAAT TGTTCCGGTG 
CAGTATGAAA CAAGAATGGC CTGTGGACTG GTGAAAGGGC ACGCCTATTC AGTCACTGGG 
CTGGAGGAGG CCCTGTTCAA AGGCGAGAAG GTGAAGCTGG TGCGGCTGCG GAACCCCTGG 
GGCCAGGTGG AGTGGAACGG CTCTTGGAGT GATGGTTGGA AGGACTGGAG CTTTGTGGAC 
AAAGACGAGA AGGCCCGTCT GCAGCACCAG GTCACCGAGG ATGGAGAGTT CTGGATGTCA 
TATGATGACT TTGTCTACCA TTTCACAAAG CTGGAGATCT GCAACCTCAC AGCTGATGCC 
CTGGAGTCCG ATAAGCTTCA GACCTGGACA GTGTCTGTAA ATGAGGGCCG CTGGGTGAGG 
GGCTGTTCTG CTGGAGGCTG CCGGAACTTC CCAGACACTT TCTGGACCAA CCCGCAGTAC 
CGTCTCAAGC TCCTGGAGGA GGATGATGAC CCTGATGACT CTGAGGTGAT TTGCAGCTTC 
CTCGTGGCTC TGATGCAGAA AAATCGGCGC AAGGACCGGA AGCTGGGGGC CAACCTCTTC 
ACCATTGGCT TCGCTATCTA CGAGGTTCCC AAAGAGATGC ACGGGAATAA GCAACACCTG 
CAGAAGGACT TCTTCTTGTA CAATGCCTCC AAGGCCAGGA GCAAAACCTA CATCAACATG 
CGGGAGGTGT CCCAGCGCTT CCGCCTGCCG CCCAGCGAGT ATGTCATTGT CCCCTCCACT 
TACGAGCCCC ATCAGGAGGG GGAATTCATC CTCCGGGTCT TCTCTGAAAA GAGGAATCTC 
TCTGAGGAAG CTGAGAATAC AATCTCTGTG GACCGGCCAG TGAAAAAGAA AAAAAACAAG 
CCCATCATCT TCGTTTCAGA CAGAGCAAAC AGCAACAAGG AGCTGGGTGT GGACCAGGAG 
GCAGAGGAGG GCAAAGACAA AACAGGGCCG GATAAACAAG GGGAAAGCCC ACAGCCACGG 
CCTGGCCACA CAGACCAGGA GAGTGAGGAG CAGCAGCAAT TCCGGAACAT CTTCAGGCAG 
ATTGCAGGCG ACGACATGGA GATCTGTGCG GATGAACTCA AGAATGTCCT TAATACGGTG 
GTGAACAAAC ACAAGGACCT GAAGACACAA GGGTTCACTC TGGAGTCCTG CAGAAGCATG 
ATAGCTCTCA TGGATACAGA TGGCTCTGGG AGACTGAATC TTCAAGAGTT CCATCACCTC 
TGGAAAAAGA TCAAGGCCTG GCAGAAAATC TTCAAACACT ATGACACTGA CCATTCTGGT 
ACCATCAATA GCTATGAGAT GCGAAATGCA GTCAATGATG CAGGCTTCCA TCTCAACAGC 
CAACTCTATG ACATCATCAC CATGCGCTAC GCAGACAAAC ACATGAACAT CGACTTTGAC 
AGCTTCATCT GCTGCTTCGT CAGGCTGGAA GGGATGTTCA GAGCTTTTCA CGCATTTGAC 
AAGGATGGAG ATGGCATCAT CAAACTGAAC GTACTTGAGT GGCTGCAGCT TACCATGTAT 
GCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017446997.1
CDS	87..2492
Translation	MPYLLPGFFCDRVIRERDRRNGEGTVSQPLKFEGQDFVVLKQRCLAQKCLFEDRVFPAGTQALGSHELSQKAKMKAITWKRPKEICENPRFIIGGANRTDICQGDLGDCWFLAAIACLTLNERLLFRVIPHDQSFTENYAGIFHFQFWRYGDWVDVVIDDCLPTYNNQLVFTKSNHRNEFWSALLEKAYAKLHGSYEALKGGNTTEAMEDFTGGVTEFFEIKDAPSDMYKIMRKAIERGSLMGCSIDDGTNMTYGTSPSGLNMGELIARMVRNMDNSLLRDSDLDPRASDDRPSRTIVPVQYETRMACGLVKGHAYSVTGLEEALFKGEKVKLVRLRNPWGQVEWNGSWSDGWKDWSFVDKDEKARLQHQVTEDGEFWMSYDDFVYHFTKLEICNLTADALESDKLQTWTVSVNEGRWVRGCSAGGCRNFPDTFWTNPQYRLKLLEEDDDPDDSEVICSFLVALMQKNRRKDRKLGANLFTIGFAIYEVPKEMHGNKQHLQKDFFLYNASKARSKTYINMREVSQRFRLPPSEYVIVPSTYEPHQEGEFILRVFSEKRNLSEEAENTISVDRPVKKKKNKPIIFVSDRANSNKELGVDQEAEEGKDKTGPDKQGESPQPRPGHTDQESEEQQQFRNIFRQIAGDDMEICADELKNVLNTVVNKHKDLKTQGFTLESCRSMIALMDTDGSGRLNLQEFHHLWKKIKAWQKIFKHYDTDHSGTINSYEMRNAVNDAGFHLNSQLYDIITMRYADKHMNIDFDSFICCFVRLEGMFRAFHAFDKDGDGIIKLNVLEWLQLTMYA

Target ORF information:

RefSeq Version	XM_017591508.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus calpain 3 (Capn3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017591508.1

ORF Insert Sequence:

ATGCCCTACC TGCTGCCGGG ATTCTTCTGT GACAGAGTGA TCAGAGAAAG GGACAGGAGA 
AATGGAGAGG GCACCGTCTC ACAGCCTCTC AAGTTTGAGG GGCAGGATTT TGTCGTTCTC 
AAACAACGGT GTCTGGCTCA GAAGTGCCTC TTTGAAGATC GAGTCTTCCC AGCAGGTACA 
CAGGCCCTTG GCTCACATGA GCTGAGCCAG AAAGCCAAGA TGAAGGCCAT CACTTGGAAG 
AGGCCAAAGG AAATTTGTGA GAATCCCCGA TTTATCATTG GTGGAGCCAA CAGGACTGAC 
ATCTGCCAAG GAGATCTAGG GGACTGCTGG TTTCTTGCAG CCATTGCCTG TCTGACCCTG 
AATGAGCGAC TGCTTTTCCG AGTTATACCT CATGATCAAA GTTTCACTGA AAACTACGCA 
GGGATCTTCC ACTTCCAGTT CTGGCGCTAT GGAGACTGGG TAGATGTGGT TATTGACGAC 
TGTCTGCCGA CATACAACAA CCAGCTGGTC TTCACCAAAT CCAACCACCG CAATGAGTTC 
TGGAGTGCTC TACTGGAGAA AGCATATGCC AAGCTCCATG GTTCCTATGA AGCTCTGAAA 
GGTGGGAACA CCACAGAAGC CATGGAGGAC TTCACAGGAG GGGTGACAGA GTTTTTTGAG 
ATCAAGGATG CTCCGAGTGA CATGTACAAG ATCATGAGGA AAGCTATCGA GAGAGGCTCC 
CTCATGGGCT GCTCCATTGA TGATGGCACC AACATGACTT ATGGAACCTC TCCTTCTGGT 
CTGAACATGG GGGAATTGAT TGCGCGGATG GTGAGAAATA TGGATAACTC GCTGCTCAGA 
GACTCAGACC TGGACCCCAG GGCCTCAGAT GACAGACCGT CACGGACAAT TGTTCCGGTG 
CAGTATGAAA CAAGAATGGC CTGTGGACTG GTGAAAGGGC ACGCCTATTC AGTCACTGGG 
CTGGAGGAGG CCCTGTTCAA AGGCGAGAAG GTGAAGCTGG TGCGGCTGCG GAACCCCTGG 
GGCCAGGTGG AGTGGAACGG CTCTTGGAGT GATGGTTGGA AGGACTGGAG CTTTGTGGAC 
AAAGACGAGA AGGCCCGTCT GCAGCACCAG GTCACCGAGG ATGGAGAGTT CTGGATGTCA 
TATGATGACT TTGTCTACCA TTTCACAAAG CTGGAGATCT GCAACCTCAC AGCTGATGCC 
CTGGAGTCCG ATAAGCTTCA GACCTGGACA GTGTCTGTAA ATGAGGGCCG CTGGGTGAGG 
GGCTGTTCTG CTGGAGGCTG CCGGAACTTC CCAGACACTT TCTGGACCAA CCCGCAGTAC 
CGTCTCAAGC TCCTGGAGGA GGATGATGAC CCTGATGACT CTGAGGTGAT TTGCAGCTTC 
CTCGTGGCTC TGATGCAGAA AAATCGGCGC AAGGACCGGA AGCTGGGGGC CAACCTCTTC 
ACCATTGGCT TCGCTATCTA CGAGGTTCCC AAAGAGATGC ACGGGAATAA GCAACACCTG 
CAGAAGGACT TCTTCTTGTA CAATGCCTCC AAGGCCAGGA GCAAAACCTA CATCAACATG 
CGGGAGGTGT CCCAGCGCTT CCGCCTGCCG CCCAGCGAGT ATGTCATTGT CCCCTCCACT 
TACGAGCCCC ATCAGGAGGG GGAATTCATC CTCCGGGTCT TCTCTGAAAA GAGGAATCTC 
TCTGAGGAAG CTGAGAATAC AATCTCTGTG GACCGGCCAG TGAAAAAGAA AAAAAACAAG 
CCCATCATCT TCGTTTCAGA CAGAGCAAAC AGCAACAAGG AGCTGGGTGT GGACCAGGAG 
GCAGAGGAGG GCAAAGACAA AACAGGGCCG GATAAACAAG GGGAAAGCCC ACAGCCACGG 
CCTGGCCACA CAGACCAGGA GAGTGAGGAG CAGCAGCAAT TCCGGAACAT CTTCAGGCAG 
ATTGCAGGCG ACGACATGGA GATCTGTGCG GATGAACTCA AGAATGTCCT TAATACGGTG 
GTGAACAAAC ACAAGGACCT GAAGACACAA GGGTTCACTC TGGAGTCCTG CAGAAGCATG 
ATAGCTCTCA TGGATACAGA TGGCTCTGGG AGACTGAATC TTCAAGAGTT CCATCACCTC 
TGGAAAAAGA TCAAGGCCTG GCAGAAAATC TTCAAACACT ATGACACTGA CCATTCTGGT 
ACCATCAATA GCTATGAGAT GCGAAATGCA GTCAATGATG CAGGCTTCCA TCTCAACAGC 
CAACTCTATG ACATCATCAC CATGCGCTAC GCAGACAAAC ACATGAACAT CGACTTTGAC 
AGCTTCATCT GCTGCTTCGT CAGGCTGGAA GGGATGTTCA GAGCTTTTCA CGCATTTGAC 
AAGGATGGAG ATGGCATCAT CAAACTGAAC GTACTTGAGT GGCTGCAGCT TACCATGTAT 
GCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Calpain 3 Expression Pattern during Gastrocnemius Muscle Atrophy and Regeneration Following Sciatic Nerve Injury in Rats.
International journal of molecular sciences16(11)26927-35(2015 Nov)
Wu R,Yan Y,Yao J,Liu Y,Zhao J,Liu M

Def defines a conserved nucleolar pathway that leads p53 to proteasome-independent degradation.
Cell research23(5)620-34(2013 May)
Tao T,Shi H,Guan Y,Huang D,Chen Y,Lane DP,Chen J,Peng J

Rbfox1 downregulation and altered calpain 3 splicing by FRG1 in a mouse model of Facioscapulohumeral muscular dystrophy (FSHD).
PLoS genetics9(1)e1003186(2013)
Pistoni M,Shiue L,Cline MS,Bortolanza S,Neguembor MV,Xynos A,Ares M Jr,Gabellini D

Calpain 3 is a rapid-action, unidirectional proteolytic switch central to muscle remodeling.
PloS one5(8)e11940(2010 Aug)
de Morr?e A,Lutje Hulsik D,Impagliazzo A,van Haagen HH,de Galan P,van Remoortere A,'t Hoen PA,van Ommen GB,Frants RR,van der Maarel SM

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Capn3 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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