BGLAP cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	BGLAP
Entrez Gene ID	281646
Full Name	bone gamma-carboxyglutamate protein
Synonyms	BGP
General protein information	Preferred Names bone gamma-carboxyglutamate protein Names osteocalcin bone Gla protein gamma-carboxyglutamic acid-containing protein
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
NM_174249.2	NP_776674.1	osteocalcin preproprotein

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	BGLAP	NP_001129100.1
	BGLAP	NP_001074237.1
Mus musculus (house mouse)	Bglap2	NP_001027469.2
Bos taurus (cattle)	BGLAP	NP_776674.1
Mus musculus (house mouse)	Bglap	NP_031567.1
Mus musculus (house mouse)	Bglap3	NP_112736.3
Rattus norvegicus (Norway rat)	Bglap	NP_038200.1
Canis lupus familiaris (dog)	BGLAP	XP_547536.2
Homo sapiens (human)	BGLAP	NP_954642.1

Gene Ontology
Bibliography

Related articles in PubMed

Osteopontin, osteocalcin and OB-cadherin expression in Synthetic nanohydroxyapatite vs bovine hydroxyapatite cultured Osteoblastic-like cells.
Santarelli A, Mascitti M, Orsini G, Mem? L, Rocchetti R, Tiriduzzi P, Sampalmieri F, Putignano A, Procaccini M, Lo Muzio L, Bambini F
Journal of biological regulators and homeostatic agents28(3)523-9(2014 Jul-Sep)

X-ray crystal structure of bovine 3 Glu-osteocalcin.
Malashkevich VN, Almo SC, Dowd TL
Biochemistry52(47)8387-92(2013 Nov)

Serum osteocalcin in dairy cows: age-related changes and periparturient variation.
Sato R, Onda K, Ochiai H, Iriki T, Yamazaki Y, Wada Y
Research in veterinary science91(2)196-8(2011 Oct)

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

The (1)H NMR structure of bovine Pb(2+)-osteocalcin and implications for lead toxicity.
Dowd TL, Li L, Gundberg CM
Biochimica et biophysica acta1784(11)1534-45(2008 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Osteopontin, osteocalcin and OB-cadherin expression in Synthetic nanohydroxyapatite vs bovine hydroxyapatite cultured Osteoblastic-like cells.
Title: Osteopontin, osteocalcin and OB-cadherin expression in Synthetic nanohydroxyapatite vs bovine hydroxyapatite cultured Osteoblastic-like cells.

Data suggest that helical content of 3 Glu-OCN is 57% and is independent of calcium binding; 3 Glu-OCN contains 3 alpha-helices around a hydrophobic core; study includes first high-resolution structure of bovine 3 Glu-OCN using X-ray crystallography.
Title: X-ray crystal structure of bovine 3 Glu-osteocalcin.

Serum osteocalcin levels in non-periparturient Holstein dairy cows were highest in the youngest cows, decreasing with age until time of first calving. In periparturient cows, serum osteocalcin levels were higher in primiparous than in multiparous cows.
Title: Serum osteocalcin in dairy cows: age-related changes and periparturient variation.

A molecular mechanism of lead toxicity for calcium binding proteins.
Title: The (1)H NMR structure of bovine Pb(2+)-osteocalcin and implications for lead toxicity.

Osteocalcin is released from the bone matrix during bone resorption as intact molecules and fragments; serum osteocalcin should be considered as a marker of bone turnover rather than bone formation
Title: Release of intact and fragmented osteocalcin molecules from bone matrix during bone resorption in vitro.

The following BGLAP gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the BGLAP cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBa44146	NM_174249.2 Latest version!	Bos taurus bone gamma-carboxyglutamate protein (BGLAP), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

BGLAP ( NM_174249.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_776674.1 Bos taurus bone gamma-carboxyglutamate protein (BGLAP), mRNA.

Price & Availability↑

CloneID	OBa44146
Clone ID Related Accession (Same CDS sequence)	NM_174249.2
Accession Version	NM_174249.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 303bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Bos taurus(cattle)
Product	osteocalcin preproprotein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from X53699.1. On Jun 3, 2003 this sequence version replaced NM_174249.1. ##Evidence-Data-START## Transcript exon combination :: X53699.1, BC126817.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145436 [ECO:0000348] ##Evidence-Data-END##

ATGAGAACCC CCATGCTGCT CGCCCTGCTG GCCCTGGCCA CACTCTGCCT CGCTGGCCGG 
GCAGATGCAA AGCCTGGTGA TGCAGAGTCG GGCAAAGGCG CAGCCTTCGT GTCCAAGCAG 
GAGGGCAGCG AGGTGGTGAA GAGACTCAGG CGCTACCTGG ACCACTGGCT GGGAGCCCCA 
GCCCCCTACC CAGATCCGCT GGAGCCCAAG AGGGAGGTGT GTGAGCTCAA CCCTGACTGT 
GACGAGCTAG CTGACCACAT CGGCTTCCAG GAAGCCTATC GGCGCTTCTA CGGCCCAGTC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_776674.1
CDS	28..330
Translation	MRTPMLLALLALATLCLAGRADAKPGDAESGKGAAFVSKQEGSEVVKRLRRYLDHWLGAPAPYPDPLEPKREVCELNPDCDELADHIGFQEAYRRFYGPV

Target ORF information:

RefSeq Version	NM_174249.2
Organism	Bos taurus(cattle)
Definition	Bos taurus bone gamma-carboxyglutamate protein (BGLAP), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_174249.2

ORF Insert Sequence:

ATGAGAACCC CCATGCTGCT CGCCCTGCTG GCCCTGGCCA CACTCTGCCT CGCTGGCCGG 
GCAGATGCAA AGCCTGGTGA TGCAGAGTCG GGCAAAGGCG CAGCCTTCGT GTCCAAGCAG 
GAGGGCAGCG AGGTGGTGAA GAGACTCAGG CGCTACCTGG ACCACTGGCT GGGAGCCCCA 
GCCCCCTACC CAGATCCGCT GGAGCCCAAG AGGGAGGTGT GTGAGCTCAA CCCTGACTGT 
GACGAGCTAG CTGACCACAT CGGCTTCCAG GAAGCCTATC GGCGCTTCTA CGGCCCAGTC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Osteopontin, osteocalcin and OB-cadherin expression in Synthetic nanohydroxyapatite vs bovine hydroxyapatite cultured Osteoblastic-like cells.
Journal of biological regulators and homeostatic agents28(3)523-9(2014 Jul-Sep)
Santarelli A,Mascitti M,Orsini G,Mem? L,Rocchetti R,Tiriduzzi P,Sampalmieri F,Putignano A,Procaccini M,Lo Muzio L,Bambini F

X-ray crystal structure of bovine 3 Glu-osteocalcin.
Biochemistry52(47)8387-92(2013 Nov)
Malashkevich VN,Almo SC,Dowd TL

Serum osteocalcin in dairy cows: age-related changes and periparturient variation.
Research in veterinary science91(2)196-8(2011 Oct)
Sato R,Onda K,Ochiai H,Iriki T,Yamazaki Y,Wada Y

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

The (1)H NMR structure of bovine Pb(2+)-osteocalcin and implications for lead toxicity.
Biochimica et biophysica acta1784(11)1534-45(2008 Nov)
Dowd TL,Li L,Gundberg CM