BCKDHB cDNA ORF clone, Bos mutus(wild yak)

Gene
Clones
gRNAs

Summary

Gene Symbol	BCKDHB
Entrez Gene ID	102284531
Full Name	branched chain keto acid dehydrogenase E1 subunit beta
Gene Type	protein-coding
Organism	Bos mutus(wild yak)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005902756.2	XP_005902818.2	2-oxoisovalerate dehydrogenase subunit beta, mitochondrial

Pathways from BioSystems
Gene Ontology

The following BCKDHB gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the BCKDHB cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBo16739	XM_005902756.2 Latest version!	Bos mutus branched chain keto acid dehydrogenase E1, beta polypeptide (BCKDHB), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$174.30 $249.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

BCKDHB ( XM_005902756.2 ) cDNA ORF clone, Bos mutus(wild yak) -> XP_005902818.2 Bos mutus branched chain keto acid dehydrogenase E1, beta polypeptide (BCKDHB), mRNA.

Price & Availability↑

CloneID	OBo16739
Clone ID Related Accession (Same CDS sequence)	XM_005902756.2
Accession Version	XM_005902756.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 870bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-10-25
Organism	Bos mutus(wild yak)
Product	2-oxoisovalerate dehydrogenase subunit beta, mitochondrial
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005394599.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Oct 26, 2015 this sequence version replaced XM_005902756.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos mutus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 3% of CDS bases ##RefSeq-Attributes-END##

ATGGTCCCAG GTCCCAATGA GTATGAATCA GGCTGTAAAG ATAGAGTTTT TAATACCCCA 
CTGTGTGAAC AAGGAATCGT TGGATTTGGA ATTGGAATCG CAGTCACCGG TGCTACTGCC 
ATAGCAGAAA TTCAGTTTGC AGATTATATT TTCCCTGCTT TTGATCAGAT TGTTAATGAA 
GCTGCCAAGT ATCGCTACCG GTCTGGGGAC CTTTTTAATT GTGGAAGCCT CACCATCCGG 
TCCCCTTGGG GCTGTGTCGG CCACGGGGCT CTCTATCATT CCCAGAGTCC TGAAGCTTTC 
TTTGCCCACT GCCCAGGAAT CAAGGTGGTT GTACCCAGAA GCCCTTTCCA GGCCAAGGGA 
CTTCTTTTAT CATGCATAGA GGATAAAAAT CCTTGTATAT TTTTTGAACC TAAAATACTT 
TACAGGGCAG CAGTGGAGCA GGTTCCTGTA GAGCCATACA ACATCCCCCT TTCCCAAGCT 
GAAGTCATCC AAGAAGGGAG TGATGTCACT CTAGTTGCCT GGGGCACTCA GGTTCATGTG 
ATCCGAGAGG TGGCTGCCAT GGCTCGAGAG AAGCTTGGGG TGTCTTGTGA GGTCATTGAT 
CTGAGGACTA TACTACCTTG GGATGTGGAT ACAGTTTGCA AGTCTGTGAT CAAAACAGGG 
CGACTGCTAG TAAGTCATGA GGCTCCTTTG ACGGGCGGCT TTGCCTCTGA GATCAGCTCA 
ACGGTTCAGG AAGAATGTTT CCTGAACCTG GAAGCTCCTA TATCAAGGGT GTGTGGGTAC 
GACACACCGT TCCCTCACAT TTTTGAACCG TTCTACATCC CAGACAAGTG GAAGTGCTAT 
GATGCCCTTC GAAAAATGAT CAACTATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005902818.2
CDS	1..870
Translation	MVPGPNEYESGCKDRVFNTPLCEQGIVGFGIGIAVTGATAIAEIQFADYIFPAFDQIVNEAAKYRYRSGDLFNCGSLTIRSPWGCVGHGALYHSQSPEAFFAHCPGIKVVVPRSPFQAKGLLLSCIEDKNPCIFFEPKILYRAAVEQVPVEPYNIPLSQAEVIQEGSDVTLVAWGTQVHVIREVAAMAREKLGVSCEVIDLRTILPWDVDTVCKSVIKTGRLLVSHEAPLTGGFASEISSTVQEECFLNLEAPISRVCGYDTPFPHIFEPFYIPDKWKCYDALRKMINY

Target ORF information:

RefSeq Version	XM_005902756.2
Organism	Bos mutus(wild yak)
Definition	Bos mutus branched chain keto acid dehydrogenase E1, beta polypeptide (BCKDHB), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005902756.2

ORF Insert Sequence:

ATGGTCCCAG GTCCCAATGA GTATGAATCA GGCTGTAAAG ATAGAGTTTT TAATACCCCA 
CTGTGTGAAC AAGGAATCGT TGGATTTGGA ATTGGAATCG CAGTCACCGG TGCTACTGCC 
ATAGCAGAAA TTCAGTTTGC AGATTATATT TTCCCTGCTT TTGATCAGAT TGTTAATGAA 
GCTGCCAAGT ATCGCTACCG GTCTGGGGAC CTTTTTAATT GTGGAAGCCT CACCATCCGG 
TCCCCTTGGG GCTGTGTCGG CCACGGGGCT CTCTATCATT CCCAGAGTCC TGAAGCTTTC 
TTTGCCCACT GCCCAGGAAT CAAGGTGGTT GTACCCAGAA GCCCTTTCCA GGCCAAGGGA 
CTTCTTTTAT CATGCATAGA GGATAAAAAT CCTTGTATAT TTTTTGAACC TAAAATACTT 
TACAGGGCAG CAGTGGAGCA GGTTCCTGTA GAGCCATACA ACATCCCCCT TTCCCAAGCT 
GAAGTCATCC AAGAAGGGAG TGATGTCACT CTAGTTGCCT GGGGCACTCA GGTTCATGTG 
ATCCGAGAGG TGGCTGCCAT GGCTCGAGAG AAGCTTGGGG TGTCTTGTGA GGTCATTGAT 
CTGAGGACTA TACTACCTTG GGATGTGGAT ACAGTTTGCA AGTCTGTGAT CAAAACAGGG 
CGACTGCTAG TAAGTCATGA GGCTCCTTTG ACGGGCGGCT TTGCCTCTGA GATCAGCTCA 
ACGGTTCAGG AAGAATGTTT CCTGAACCTG GAAGCTCCTA TATCAAGGGT GTGTGGGTAC 
GACACACCGT TCCCTCACAT TTTTGAACCG TTCTACATCC CAGACAAGTG GAAGTGCTAT 
GATGCCCTTC GAAAAATGAT CAACTATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.