DNMT3A cDNA ORF clone, Bos indicus(Bos taurus indicus)

Gene
Clones
gRNAs

Summary

Gene Symbol	DNMT3A
Entrez Gene ID	109566324
Full Name	DNA methyltransferase 3 alpha
Gene Type	protein-coding
Organism	Bos indicus(Bos taurus indicus)

mRNA and Protein(s)

mRNA	Protein	Name
XM_019970606.1	XP_019826165.1	DNA (cytosine-5)-methyltransferase 3A
XM_019970605.1	XP_019826164.1	DNA (cytosine-5)-methyltransferase 3A

Pathways from BioSystems
Gene Ontology

The following DNMT3A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DNMT3A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBo29330	XM_019970605.1 Latest version!	Bos indicus DNA methyltransferase 3 alpha (DNMT3A), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OBo29330	XM_019970606.1 Latest version!	Bos indicus DNA methyltransferase 3 alpha (DNMT3A), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DNMT3A ( XM_019970606.1 ) cDNA ORF clone, Bos indicus(Bos taurus indicus) -> XP_019826165.1 Bos indicus DNA methyltransferase 3 alpha (DNMT3A), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBo29330
Clone ID Related Accession (Same CDS sequence)	XM_019970606.1 , XM_019970605.1
Accession Version	XM_019970606.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2730bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-01-11
Organism	Bos indicus(Bos taurus indicus)
Product	DNA (cytosine-5)-methyltransferase 3A
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_032660.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos indicus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCGCCA GCGGCCCCGG GGACACCAGC GGCTCCGCTC TGGAGCGGGA GGAGGAGCGC 
AAGGAGGGAG AGGAGCAGGA GGAGGCTCGT GCCAAGGAGG AGCGGCAGGA GCCCAGCACA 
ACTGCCCGGA AGGTGGGGCG GCCTGGCAGG AAGCGCAAGC ACCCCCCGGT GGAAAGCAGT 
GACACACCGA AGGACCCCGC CGTGACCTCC AAGTCCCTGT CCATGGCCCA AGACTCAGGC 
CCCTCAGAGC TGTTACCCAA CGGGGACTTG GAGAAGCGGA GTGAGCCCCA GCCAGAGGAG 
GGGAGCCCCG CTGGAGGGCA GAAGGGCGGG GCCCCAGCAG AGGGAGAGGG TGCCACTGAG 
ACCCCGCCCG AAGCCTCCAG AGCTGTAGAR AATGGCTGCT GCACCCCCAA GGATGGCCGG 
GGAGCCCCTG CAGAAGAGGG CAAAGAACAG AAGGAGACCA ACATCGAATC CATGAAAATG 
GAGGGCTCCC GGGGCCGGCT GCGGGGTGGC CTGGGCTGGG AGTCCAGCCT CCGCCAGCGG 
CCCATGCCGC GGCTCACCTT CCAGGCGGGG GACCCCTACT ACATCAGCAA GCGCAAGCGG 
GACGAGTGGC TGGCACGCTG GAAAAGGGAG GCTGAGAAGA AAGCCAAGGT GATCGCAGTA 
ATGAATGCTG TTGAAGAAAA CCAGGGGTCC ACCGAGTCTC AGAAGGTGGA GGAGGCCAGT 
CCTCCCGCTG TGCAGCAGCC CACCGACCCT GCGTCCCCCA CAGTGGCCAC CACGCCTGAG 
CCCGTGGGCG CCGATGCCGG GGACAAGAAT GCCACCAAAG CAGCTGACGA TGAACCGGAG 
TACGAGGACG GCCGGGGCTT TGGCATTGGG GAGCTGGTGT GGGGGAAACT GCGGGGCTTC 
TCCTGGTGGC CAGGCCGCAT TGTGTCTTGG TGGATGACGG GCCGGAGCCG AGCAGCGGAA 
GGCACTCGTT GGGTCATGTG GTTCGGAGAC GGCAAGTTCT CAGTGGTGTG TGTGGAGAAG 
CTGATGCCGC TGAGCTCCTT CTGCAGTGCT TTCCACCAGG CCACCTACAA CAAGCAGCCC 
ATGTACCGCA AGGCTATCTA CGAGGTCCTG CAGGTGGCCA GCAGTCGAGC GGGGAAGCTG 
TTTCCAATGT GCCATGACAG CGACGAGAGC GACACTGCCA AGGCCGTGGA GGTGCAGAAC 
AAACARATGA TCGAGTGGGC CCTGGGAGGG TTCCAGCCCT CTGGCCCCAA GGGCCTGGAG 
CCCCCAGAAG AGGAGAAGAA CCCCTACAAA GAAGTTTACA CAGACATGTG GGTTGAACCC 
GAGGCAGCTG CCTATGCGCC GCCCCCACCA GCCAAAAAGC CCCGAAAGAG CACAACGGAG 
AAGCCTAAGG TCAAGGAGAT CATTGATGAA CGCACAAGAG AGCGGCTGGT GTACGAGGTA 
CGGCAGAAGT GCCGGAACAT CGAGGACATC TGCATCTCTT GTGGGAGCCT CAACGTCACC 
TTGGAACACC CTCTCTTCAT CGGAGGAATG TGCCAAAACT GCAAGAACTG CTTCCTGGAA 
TGCGCGTACC AGTACGATGA CGATGGCTAT CAGTCCTACT GCACCATCTG CTGCGGGGGG 
CGCGAGGTGC TCATGTGTGG GAACAACAAT TGCTGCAGGT GCTTTTGCGT GGAATGTGTG 
GATCTCTTGG TGGGGCCAGG GGCYGCGCAG GCAGCCATCA AAGAAGAYCC CTGGAACTGC 
TACATGTGCG GGCACAAGGG CACCTACGGG CTGCTGCGGC GGCGGGAYGA CTGGCCGTCT 
CGGCTCCAGA TGTTCTTCGC CAACAACCAT GACCAGGAAT TCGATCCTCC GAAGGTTTAC 
CCACCTGTCC CAGCTGAGAA GAGGAAGCCC ATCCGGGTGC TGTCTCTATT CGATGGAATT 
GCTACAGGGC TTCTGGTGCT GAAGGACTTG GGCATTCAGG TGGACCGCTA CATCGCCTCC 
GAGGTGTGTG AGGACTCCAT CACGGTGGGC ATGGTGCGGC ACCAGGGAAA GATCATGTAC 
GTCGGGGACG TCCGCAGCGT TACACAGAAG CATATCCAGG AATGGGGCCC GTTCGATCTG 
GTGATTGGGG GCAGTCCCTG CAATGATCTC TCCATCGTCA ACCCTGCCCG CAAGGGACTC 
TACGAGGGCA CTGGCCGGCT CTTCTTTGAG TTCTACCGCC TCCTGCATGA TGCGCGGCCC 
AAGGAGGGAG ATGACCGCCC CTTCTTCTGG CTCTTTGAGA ATGTGGTGGC CATGGGCGTT 
AGTGACAAGA GGGACATCTC GCGATTTCTC GAGTCCAACC CTGTGATGAT TGATGCCAAA 
GAAGTGTCAG CTGCGCACAG GGCCCGCTAC TTCTGGGGGA ACCTTCCTGG TATGAACAGG 
CCATTGGCAT CCACTGTGAA TGATAAGCTG GAGCTGCAGG AGTGTCTGGA GCACGGCCGA 
ATAGCCAAGT TCAGCAAAGT GAGGACCATT ACTACTAGGT CGAACTCCAT AAAGCAGGGC 
AAAGACCAGC ATTTCCCCGT CTTCATGAAT GAGAAAGAGG ACATCTTATG GTGCACTGAA 
ATGGAAAGGG TGTTTGGCTT CCCTGTCCAC TATACCGACG TCTCCAACAT GAGCCGCTTG 
GCGAGGCAGA GACTGCTGGG CCGGTCGTGG AGCGTGCCGG TCATCCGCCA CCTCTTCGCT 
CCGCTGAAGG AATATTTTGC TTGTGTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019826165.1
CDS	1250..3979
Translation	MPASGPGDTSGSALEREEERKEGEEQEEARAKEERQEPSTTARKVGRPGRKRKHPPVESSDTPKDPAVTSKSLSMAQDSGPSELLPNGDLEKRSEPQPEEGSPAGGQKGGAPAEGEGATETPPEASRAVnullNGCCTPKDGRGAPAEEGKEQKETNIESMKMEGSRGRLRGGLGWESSLRQRPMPRLTFQAGDPYYISKRKRDEWLARWKREAEKKAKVIAVMNAVEENQGSTESQKVEEASPPAVQQPTDPASPTVATTPEPVGADAGDKNATKAADDEPEYEDGRGFGIGELVWGKLRGFSWWPGRIVSWWMTGRSRAAEGTRWVMWFGDGKFSVVCVEKLMPLSSFCSAFHQATYNKQPMYRKAIYEVLQVASSRAGKLFPMCHDSDESDTAKAVEVQNKnullMIEWALGGFQPSGPKGLEPPEEEKNPYKEVYTDMWVEPEAAAYAPPPPAKKPRKSTTEKPKVKEIIDERTRERLVYEVRQKCRNIEDICISCGSLNVTLEHPLFIGGMCQNCKNCFLECAYQYDDDGYQSYCTICCGGREVLMCGNNNCCRCFCVECVDLLVGPGnullAQAAIKEnullPWNCYMCGHKGTYGLLRRRnullDWPSRLQMFFANNHDQEFDPPKVYPPVPAEKRKPIRVLSLFDGIATGLLVLKDLGIQVDRYIASEVCEDSITVGMVRHQGKIMYVGDVRSVTQKHIQEWGPFDLVIGGSPCNDLSIVNPARKGLYEGTGRLFFEFYRLLHDARPKEGDDRPFFWLFENVVAMGVSDKRDISRFLESNPVMIDAKEVSAAHRARYFWGNLPGMNRPLASTVNDKLELQECLEHGRIAKFSKVRTITTRSNSIKQGKDQHFPVFMNEKEDILWCTEMERVFGFPVHYTDVSNMSRLARQRLLGRSWSVPVIRHLFAPLKEYFACV

Target ORF information:

RefSeq Version	XM_019970606.1
Organism	Bos indicus(Bos taurus indicus)
Definition	Bos indicus DNA methyltransferase 3 alpha (DNMT3A), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019970606.1

ORF Insert Sequence:

ATGCCCGCCA GCGGCCCCGG GGACACCAGC GGCTCCGCTC TGGAGCGGGA GGAGGAGCGC 
AAGGAGGGAG AGGAGCAGGA GGAGGCTCGT GCCAAGGAGG AGCGGCAGGA GCCCAGCACA 
ACTGCCCGGA AGGTGGGGCG GCCTGGCAGG AAGCGCAAGC ACCCCCCGGT GGAAAGCAGT 
GACACACCGA AGGACCCCGC CGTGACCTCC AAGTCCCTGT CCATGGCCCA AGACTCAGGC 
CCCTCAGAGC TGTTACCCAA CGGGGACTTG GAGAAGCGGA GTGAGCCCCA GCCAGAGGAG 
GGGAGCCCCG CTGGAGGGCA GAAGGGCGGG GCCCCAGCAG AGGGAGAGGG TGCCACTGAG 
ACCCCGCCCG AAGCCTCCAG AGCTGTAGAR AATGGCTGCT GCACCCCCAA GGATGGCCGG 
GGAGCCCCTG CAGAAGAGGG CAAAGAACAG AAGGAGACCA ACATCGAATC CATGAAAATG 
GAGGGCTCCC GGGGCCGGCT GCGGGGTGGC CTGGGCTGGG AGTCCAGCCT CCGCCAGCGG 
CCCATGCCGC GGCTCACCTT CCAGGCGGGG GACCCCTACT ACATCAGCAA GCGCAAGCGG 
GACGAGTGGC TGGCACGCTG GAAAAGGGAG GCTGAGAAGA AAGCCAAGGT GATCGCAGTA 
ATGAATGCTG TTGAAGAAAA CCAGGGGTCC ACCGAGTCTC AGAAGGTGGA GGAGGCCAGT 
CCTCCCGCTG TGCAGCAGCC CACCGACCCT GCGTCCCCCA CAGTGGCCAC CACGCCTGAG 
CCCGTGGGCG CCGATGCCGG GGACAAGAAT GCCACCAAAG CAGCTGACGA TGAACCGGAG 
TACGAGGACG GCCGGGGCTT TGGCATTGGG GAGCTGGTGT GGGGGAAACT GCGGGGCTTC 
TCCTGGTGGC CAGGCCGCAT TGTGTCTTGG TGGATGACGG GCCGGAGCCG AGCAGCGGAA 
GGCACTCGTT GGGTCATGTG GTTCGGAGAC GGCAAGTTCT CAGTGGTGTG TGTGGAGAAG 
CTGATGCCGC TGAGCTCCTT CTGCAGTGCT TTCCACCAGG CCACCTACAA CAAGCAGCCC 
ATGTACCGCA AGGCTATCTA CGAGGTCCTG CAGGTGGCCA GCAGTCGAGC GGGGAAGCTG 
TTTCCAATGT GCCATGACAG CGACGAGAGC GACACTGCCA AGGCCGTGGA GGTGCAGAAC 
AAACARATGA TCGAGTGGGC CCTGGGAGGG TTCCAGCCCT CTGGCCCCAA GGGCCTGGAG 
CCCCCAGAAG AGGAGAAGAA CCCCTACAAA GAAGTTTACA CAGACATGTG GGTTGAACCC 
GAGGCAGCTG CCTATGCGCC GCCCCCACCA GCCAAAAAGC CCCGAAAGAG CACAACGGAG 
AAGCCTAAGG TCAAGGAGAT CATTGATGAA CGCACAAGAG AGCGGCTGGT GTACGAGGTA 
CGGCAGAAGT GCCGGAACAT CGAGGACATC TGCATCTCTT GTGGGAGCCT CAACGTCACC 
TTGGAACACC CTCTCTTCAT CGGAGGAATG TGCCAAAACT GCAAGAACTG CTTCCTGGAA 
TGCGCGTACC AGTACGATGA CGATGGCTAT CAGTCCTACT GCACCATCTG CTGCGGGGGG 
CGCGAGGTGC TCATGTGTGG GAACAACAAT TGCTGCAGGT GCTTTTGCGT GGAATGTGTG 
GATCTCTTGG TGGGGCCAGG GGCYGCGCAG GCAGCCATCA AAGAAGAYCC CTGGAACTGC 
TACATGTGCG GGCACAAGGG CACCTACGGG CTGCTGCGGC GGCGGGAYGA CTGGCCGTCT 
CGGCTCCAGA TGTTCTTCGC CAACAACCAT GACCAGGAAT TCGATCCTCC GAAGGTTTAC 
CCACCTGTCC CAGCTGAGAA GAGGAAGCCC ATCCGGGTGC TGTCTCTATT CGATGGAATT 
GCTACAGGGC TTCTGGTGCT GAAGGACTTG GGCATTCAGG TGGACCGCTA CATCGCCTCC 
GAGGTGTGTG AGGACTCCAT CACGGTGGGC ATGGTGCGGC ACCAGGGAAA GATCATGTAC 
GTCGGGGACG TCCGCAGCGT TACACAGAAG CATATCCAGG AATGGGGCCC GTTCGATCTG 
GTGATTGGGG GCAGTCCCTG CAATGATCTC TCCATCGTCA ACCCTGCCCG CAAGGGACTC 
TACGAGGGCA CTGGCCGGCT CTTCTTTGAG TTCTACCGCC TCCTGCATGA TGCGCGGCCC 
AAGGAGGGAG ATGACCGCCC CTTCTTCTGG CTCTTTGAGA ATGTGGTGGC CATGGGCGTT 
AGTGACAAGA GGGACATCTC GCGATTTCTC GAGTCCAACC CTGTGATGAT TGATGCCAAA 
GAAGTGTCAG CTGCGCACAG GGCCCGCTAC TTCTGGGGGA ACCTTCCTGG TATGAACAGG 
CCATTGGCAT CCACTGTGAA TGATAAGCTG GAGCTGCAGG AGTGTCTGGA GCACGGCCGA 
ATAGCCAAGT TCAGCAAAGT GAGGACCATT ACTACTAGGT CGAACTCCAT AAAGCAGGGC 
AAAGACCAGC ATTTCCCCGT CTTCATGAAT GAGAAAGAGG ACATCTTATG GTGCACTGAA 
ATGGAAAGGG TGTTTGGCTT CCCTGTCCAC TATACCGACG TCTCCAACAT GAGCCGCTTG 
GCGAGGCAGA GACTGCTGGG CCGGTCGTGG AGCGTGCCGG TCATCCGCCA CCTCTTCGCT 
CCGCTGAAGG AATATTTTGC TTGTGTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DNMT3A ( XM_019970605.1 ) cDNA ORF clone, Bos indicus(Bos taurus indicus) -> XP_019826164.1 Bos indicus DNA methyltransferase 3 alpha (DNMT3A), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBo29330
Clone ID Related Accession (Same CDS sequence)	XM_019970606.1 , XM_019970605.1
Accession Version	XM_019970605.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2730bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-01-11
Organism	Bos indicus(Bos taurus indicus)
Product	DNA (cytosine-5)-methyltransferase 3A
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_032660.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos indicus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCGCCA GCGGCCCCGG GGACACCAGC GGCTCCGCTC TGGAGCGGGA GGAGGAGCGC 
AAGGAGGGAG AGGAGCAGGA GGAGGCTCGT GCCAAGGAGG AGCGGCAGGA GCCCAGCACA 
ACTGCCCGGA AGGTGGGGCG GCCTGGCAGG AAGCGCAAGC ACCCCCCGGT GGAAAGCAGT 
GACACACCGA AGGACCCCGC CGTGACCTCC AAGTCCCTGT CCATGGCCCA AGACTCAGGC 
CCCTCAGAGC TGTTACCCAA CGGGGACTTG GAGAAGCGGA GTGAGCCCCA GCCAGAGGAG 
GGGAGCCCCG CTGGAGGGCA GAAGGGCGGG GCCCCAGCAG AGGGAGAGGG TGCCACTGAG 
ACCCCGCCCG AAGCCTCCAG AGCTGTAGAR AATGGCTGCT GCACCCCCAA GGATGGCCGG 
GGAGCCCCTG CAGAAGAGGG CAAAGAACAG AAGGAGACCA ACATCGAATC CATGAAAATG 
GAGGGCTCCC GGGGCCGGCT GCGGGGTGGC CTGGGCTGGG AGTCCAGCCT CCGCCAGCGG 
CCCATGCCGC GGCTCACCTT CCAGGCGGGG GACCCCTACT ACATCAGCAA GCGCAAGCGG 
GACGAGTGGC TGGCACGCTG GAAAAGGGAG GCTGAGAAGA AAGCCAAGGT GATCGCAGTA 
ATGAATGCTG TTGAAGAAAA CCAGGGGTCC ACCGAGTCTC AGAAGGTGGA GGAGGCCAGT 
CCTCCCGCTG TGCAGCAGCC CACCGACCCT GCGTCCCCCA CAGTGGCCAC CACGCCTGAG 
CCCGTGGGCG CCGATGCCGG GGACAAGAAT GCCACCAAAG CAGCTGACGA TGAACCGGAG 
TACGAGGACG GCCGGGGCTT TGGCATTGGG GAGCTGGTGT GGGGGAAACT GCGGGGCTTC 
TCCTGGTGGC CAGGCCGCAT TGTGTCTTGG TGGATGACGG GCCGGAGCCG AGCAGCGGAA 
GGCACTCGTT GGGTCATGTG GTTCGGAGAC GGCAAGTTCT CAGTGGTGTG TGTGGAGAAG 
CTGATGCCGC TGAGCTCCTT CTGCAGTGCT TTCCACCAGG CCACCTACAA CAAGCAGCCC 
ATGTACCGCA AGGCTATCTA CGAGGTCCTG CAGGTGGCCA GCAGTCGAGC GGGGAAGCTG 
TTTCCAATGT GCCATGACAG CGACGAGAGC GACACTGCCA AGGCCGTGGA GGTGCAGAAC 
AAACARATGA TCGAGTGGGC CCTGGGAGGG TTCCAGCCCT CTGGCCCCAA GGGCCTGGAG 
CCCCCAGAAG AGGAGAAGAA CCCCTACAAA GAAGTTTACA CAGACATGTG GGTTGAACCC 
GAGGCAGCTG CCTATGCGCC GCCCCCACCA GCCAAAAAGC CCCGAAAGAG CACAACGGAG 
AAGCCTAAGG TCAAGGAGAT CATTGATGAA CGCACAAGAG AGCGGCTGGT GTACGAGGTA 
CGGCAGAAGT GCCGGAACAT CGAGGACATC TGCATCTCTT GTGGGAGCCT CAACGTCACC 
TTGGAACACC CTCTCTTCAT CGGAGGAATG TGCCAAAACT GCAAGAACTG CTTCCTGGAA 
TGCGCGTACC AGTACGATGA CGATGGCTAT CAGTCCTACT GCACCATCTG CTGCGGGGGG 
CGCGAGGTGC TCATGTGTGG GAACAACAAT TGCTGCAGGT GCTTTTGCGT GGAATGTGTG 
GATCTCTTGG TGGGGCCAGG GGCYGCGCAG GCAGCCATCA AAGAAGAYCC CTGGAACTGC 
TACATGTGCG GGCACAAGGG CACCTACGGG CTGCTGCGGC GGCGGGAYGA CTGGCCGTCT 
CGGCTCCAGA TGTTCTTCGC CAACAACCAT GACCAGGAAT TCGATCCTCC GAAGGTTTAC 
CCACCTGTCC CAGCTGAGAA GAGGAAGCCC ATCCGGGTGC TGTCTCTATT CGATGGAATT 
GCTACAGGGC TTCTGGTGCT GAAGGACTTG GGCATTCAGG TGGACCGCTA CATCGCCTCC 
GAGGTGTGTG AGGACTCCAT CACGGTGGGC ATGGTGCGGC ACCAGGGAAA GATCATGTAC 
GTCGGGGACG TCCGCAGCGT TACACAGAAG CATATCCAGG AATGGGGCCC GTTCGATCTG 
GTGATTGGGG GCAGTCCCTG CAATGATCTC TCCATCGTCA ACCCTGCCCG CAAGGGACTC 
TACGAGGGCA CTGGCCGGCT CTTCTTTGAG TTCTACCGCC TCCTGCATGA TGCGCGGCCC 
AAGGAGGGAG ATGACCGCCC CTTCTTCTGG CTCTTTGAGA ATGTGGTGGC CATGGGCGTT 
AGTGACAAGA GGGACATCTC GCGATTTCTC GAGTCCAACC CTGTGATGAT TGATGCCAAA 
GAAGTGTCAG CTGCGCACAG GGCCCGCTAC TTCTGGGGGA ACCTTCCTGG TATGAACAGG 
CCATTGGCAT CCACTGTGAA TGATAAGCTG GAGCTGCAGG AGTGTCTGGA GCACGGCCGA 
ATAGCCAAGT TCAGCAAAGT GAGGACCATT ACTACTAGGT CGAACTCCAT AAAGCAGGGC 
AAAGACCAGC ATTTCCCCGT CTTCATGAAT GAGAAAGAGG ACATCTTATG GTGCACTGAA 
ATGGAAAGGG TGTTTGGCTT CCCTGTCCAC TATACCGACG TCTCCAACAT GAGCCGCTTG 
GCGAGGCAGA GACTGCTGGG CCGGTCGTGG AGCGTGCCGG TCATCCGCCA CCTCTTCGCT 
CCGCTGAAGG AATATTTTGC TTGTGTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019826164.1
CDS	357..3086
Translation	MPASGPGDTSGSALEREEERKEGEEQEEARAKEERQEPSTTARKVGRPGRKRKHPPVESSDTPKDPAVTSKSLSMAQDSGPSELLPNGDLEKRSEPQPEEGSPAGGQKGGAPAEGEGATETPPEASRAVnullNGCCTPKDGRGAPAEEGKEQKETNIESMKMEGSRGRLRGGLGWESSLRQRPMPRLTFQAGDPYYISKRKRDEWLARWKREAEKKAKVIAVMNAVEENQGSTESQKVEEASPPAVQQPTDPASPTVATTPEPVGADAGDKNATKAADDEPEYEDGRGFGIGELVWGKLRGFSWWPGRIVSWWMTGRSRAAEGTRWVMWFGDGKFSVVCVEKLMPLSSFCSAFHQATYNKQPMYRKAIYEVLQVASSRAGKLFPMCHDSDESDTAKAVEVQNKnullMIEWALGGFQPSGPKGLEPPEEEKNPYKEVYTDMWVEPEAAAYAPPPPAKKPRKSTTEKPKVKEIIDERTRERLVYEVRQKCRNIEDICISCGSLNVTLEHPLFIGGMCQNCKNCFLECAYQYDDDGYQSYCTICCGGREVLMCGNNNCCRCFCVECVDLLVGPGnullAQAAIKEnullPWNCYMCGHKGTYGLLRRRnullDWPSRLQMFFANNHDQEFDPPKVYPPVPAEKRKPIRVLSLFDGIATGLLVLKDLGIQVDRYIASEVCEDSITVGMVRHQGKIMYVGDVRSVTQKHIQEWGPFDLVIGGSPCNDLSIVNPARKGLYEGTGRLFFEFYRLLHDARPKEGDDRPFFWLFENVVAMGVSDKRDISRFLESNPVMIDAKEVSAAHRARYFWGNLPGMNRPLASTVNDKLELQECLEHGRIAKFSKVRTITTRSNSIKQGKDQHFPVFMNEKEDILWCTEMERVFGFPVHYTDVSNMSRLARQRLLGRSWSVPVIRHLFAPLKEYFACV

Target ORF information:

RefSeq Version	XM_019970605.1
Organism	Bos indicus(Bos taurus indicus)
Definition	Bos indicus DNA methyltransferase 3 alpha (DNMT3A), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019970605.1

ORF Insert Sequence:

ATGCCCGCCA GCGGCCCCGG GGACACCAGC GGCTCCGCTC TGGAGCGGGA GGAGGAGCGC 
AAGGAGGGAG AGGAGCAGGA GGAGGCTCGT GCCAAGGAGG AGCGGCAGGA GCCCAGCACA 
ACTGCCCGGA AGGTGGGGCG GCCTGGCAGG AAGCGCAAGC ACCCCCCGGT GGAAAGCAGT 
GACACACCGA AGGACCCCGC CGTGACCTCC AAGTCCCTGT CCATGGCCCA AGACTCAGGC 
CCCTCAGAGC TGTTACCCAA CGGGGACTTG GAGAAGCGGA GTGAGCCCCA GCCAGAGGAG 
GGGAGCCCCG CTGGAGGGCA GAAGGGCGGG GCCCCAGCAG AGGGAGAGGG TGCCACTGAG 
ACCCCGCCCG AAGCCTCCAG AGCTGTAGAR AATGGCTGCT GCACCCCCAA GGATGGCCGG 
GGAGCCCCTG CAGAAGAGGG CAAAGAACAG AAGGAGACCA ACATCGAATC CATGAAAATG 
GAGGGCTCCC GGGGCCGGCT GCGGGGTGGC CTGGGCTGGG AGTCCAGCCT CCGCCAGCGG 
CCCATGCCGC GGCTCACCTT CCAGGCGGGG GACCCCTACT ACATCAGCAA GCGCAAGCGG 
GACGAGTGGC TGGCACGCTG GAAAAGGGAG GCTGAGAAGA AAGCCAAGGT GATCGCAGTA 
ATGAATGCTG TTGAAGAAAA CCAGGGGTCC ACCGAGTCTC AGAAGGTGGA GGAGGCCAGT 
CCTCCCGCTG TGCAGCAGCC CACCGACCCT GCGTCCCCCA CAGTGGCCAC CACGCCTGAG 
CCCGTGGGCG CCGATGCCGG GGACAAGAAT GCCACCAAAG CAGCTGACGA TGAACCGGAG 
TACGAGGACG GCCGGGGCTT TGGCATTGGG GAGCTGGTGT GGGGGAAACT GCGGGGCTTC 
TCCTGGTGGC CAGGCCGCAT TGTGTCTTGG TGGATGACGG GCCGGAGCCG AGCAGCGGAA 
GGCACTCGTT GGGTCATGTG GTTCGGAGAC GGCAAGTTCT CAGTGGTGTG TGTGGAGAAG 
CTGATGCCGC TGAGCTCCTT CTGCAGTGCT TTCCACCAGG CCACCTACAA CAAGCAGCCC 
ATGTACCGCA AGGCTATCTA CGAGGTCCTG CAGGTGGCCA GCAGTCGAGC GGGGAAGCTG 
TTTCCAATGT GCCATGACAG CGACGAGAGC GACACTGCCA AGGCCGTGGA GGTGCAGAAC 
AAACARATGA TCGAGTGGGC CCTGGGAGGG TTCCAGCCCT CTGGCCCCAA GGGCCTGGAG 
CCCCCAGAAG AGGAGAAGAA CCCCTACAAA GAAGTTTACA CAGACATGTG GGTTGAACCC 
GAGGCAGCTG CCTATGCGCC GCCCCCACCA GCCAAAAAGC CCCGAAAGAG CACAACGGAG 
AAGCCTAAGG TCAAGGAGAT CATTGATGAA CGCACAAGAG AGCGGCTGGT GTACGAGGTA 
CGGCAGAAGT GCCGGAACAT CGAGGACATC TGCATCTCTT GTGGGAGCCT CAACGTCACC 
TTGGAACACC CTCTCTTCAT CGGAGGAATG TGCCAAAACT GCAAGAACTG CTTCCTGGAA 
TGCGCGTACC AGTACGATGA CGATGGCTAT CAGTCCTACT GCACCATCTG CTGCGGGGGG 
CGCGAGGTGC TCATGTGTGG GAACAACAAT TGCTGCAGGT GCTTTTGCGT GGAATGTGTG 
GATCTCTTGG TGGGGCCAGG GGCYGCGCAG GCAGCCATCA AAGAAGAYCC CTGGAACTGC 
TACATGTGCG GGCACAAGGG CACCTACGGG CTGCTGCGGC GGCGGGAYGA CTGGCCGTCT 
CGGCTCCAGA TGTTCTTCGC CAACAACCAT GACCAGGAAT TCGATCCTCC GAAGGTTTAC 
CCACCTGTCC CAGCTGAGAA GAGGAAGCCC ATCCGGGTGC TGTCTCTATT CGATGGAATT 
GCTACAGGGC TTCTGGTGCT GAAGGACTTG GGCATTCAGG TGGACCGCTA CATCGCCTCC 
GAGGTGTGTG AGGACTCCAT CACGGTGGGC ATGGTGCGGC ACCAGGGAAA GATCATGTAC 
GTCGGGGACG TCCGCAGCGT TACACAGAAG CATATCCAGG AATGGGGCCC GTTCGATCTG 
GTGATTGGGG GCAGTCCCTG CAATGATCTC TCCATCGTCA ACCCTGCCCG CAAGGGACTC 
TACGAGGGCA CTGGCCGGCT CTTCTTTGAG TTCTACCGCC TCCTGCATGA TGCGCGGCCC 
AAGGAGGGAG ATGACCGCCC CTTCTTCTGG CTCTTTGAGA ATGTGGTGGC CATGGGCGTT 
AGTGACAAGA GGGACATCTC GCGATTTCTC GAGTCCAACC CTGTGATGAT TGATGCCAAA 
GAAGTGTCAG CTGCGCACAG GGCCCGCTAC TTCTGGGGGA ACCTTCCTGG TATGAACAGG 
CCATTGGCAT CCACTGTGAA TGATAAGCTG GAGCTGCAGG AGTGTCTGGA GCACGGCCGA 
ATAGCCAAGT TCAGCAAAGT GAGGACCATT ACTACTAGGT CGAACTCCAT AAAGCAGGGC 
AAAGACCAGC ATTTCCCCGT CTTCATGAAT GAGAAAGAGG ACATCTTATG GTGCACTGAA 
ATGGAAAGGG TGTTTGGCTT CCCTGTCCAC TATACCGACG TCTCCAACAT GAGCCGCTTG 
GCGAGGCAGA GACTGCTGGG CCGGTCGTGG AGCGTGCCGG TCATCCGCCA CCTCTTCGCT 
CCGCTGAAGG AATATTTTGC TTGTGTGTAA