LOC100643758 cDNA ORF clone, Bombus terrestris(buff-tailed bumblebee)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC100643758
Entrez Gene ID	100643758
Full Name	E3 ubiquitin-protein ligase MIB1
Gene Type	protein-coding
Organism	Bombus terrestris(buff-tailed bumblebee)

mRNA and Protein(s)

mRNA	Protein	Name
XM_020868431.1	XP_020724090.1	E3 ubiquitin-protein ligase MIB1

Pathways from BioSystems
Gene Ontology

The following LOC100643758 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC100643758 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBo66376	XM_020868431.1 Latest version!	Bombus terrestris E3 ubiquitin-protein ligase MIB1 (LOC100643758), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC100643758 ( XM_020868431.1 ) cDNA ORF clone, Bombus terrestris(buff-tailed bumblebee) -> XP_020724090.1 Bombus terrestris E3 ubiquitin-protein ligase MIB1 (LOC100643758), mRNA.

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CloneID	OBo66376
Clone ID Related Accession (Same CDS sequence)	XM_020868431.1
Accession Version	XM_020868431.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1926bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-04-24
Organism	Bombus terrestris(buff-tailed bumblebee)
Product	E3 ubiquitin-protein ligase MIB1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_015764.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bombus terrestris Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGCTG GTGCGAGTAC CGGTACTCGT ACAACGCGAT TTATGATGGA GGGTGTCGGA 
GCCCGAGTCA TCCGTGGACC CGAATGGAAG TGGGGTAAAC AGGATGGAGG AGAAGGTCAT 
GTAGGAACTG TTAGAAATTT TGAATCTCCA GAAGAAGTAG TTGTTGTTTG GGATAATGGT 
ACAGCAGCTA ATTATCGATG TTCTGGTGCA TTTGATCTTC GAATTTTGGA TTCAGCTCCG 
ACTGGCGTAA AACATGATGG AACAATGTGT GATACGTGCA GACAACAACC AATTTTTGGA 
ATTCGTTGGA AATGTGCAGA ATGTGGGAAT TATGACCTTT GTTCTATTTG TTATCACGGA 
GACAAGCATC ACTTAAGACA TAGATTTTAT CGTATAGCAA CACCTGGAAG TGAAAGAGTA 
TTATTGGAGC CACGACGTAA AAGCAAAAAG ATTGCAATTC GTGGTATATT TCCTGGTGCC 
AGAGTTGTAA GAGGTGTTGA TTGGCAGTGG GAAGATCAAG ATGGTGGAAA TGGTCGAAGA 
GGTAAAGTTA ATGAAATTCA AGATTGGTCT GCAGCTAGTC CACGTTCGGC AGCCTATGTT 
ATATGGGATA ATGGTGCCAA AAATTTGTAT CGCGTTGGTT TTGAAGGAAT GGCGGATTTA 
AAAGTTGTTA ATGATGCTAA AGGCCAAACA GTTTACAGAT ATCATTTACC ACTATTAGGT 
GAACAGGGTC CAGGTCGCAC TGGCGCCCAT GGATTACAAA TTGGAGATCA GGTTAATGTT 
GATTTAGAAT TAGAAATCGT ACAATCGTTA CAACACGGGC ATGGTGGTTG GACGGATGGT 
ATGTTTGAGT GTCTTGGTAC TACAGGGACT GTTGTTGGTA TTGATGAAGA TCACGATATT 
GTTGTGTCCT ACCCAAGTGG AAATCGATGG ACCTTCAACC CTGCTGTATT GACAAAAGTA 
CAAATACCAG TCCCTGTTAC TAGTTCAAGT TCTGACAACC AAACATTTGC GGTTGGTGAT 
TTGGTACAAA TATGTAACGA TATAGAAAAA ATAAAACGAC TTCAACGAGG TCATGGTGAA 
TGGGCTGAAG CAATGGCACC GACTATGGGA AAGATTGGTA GAGTTTTGCA AATTTATCAC 
GATGGGGATT TAAAAGTAGA AGTTTGTAGT ACATCTTGGA CATATAATCC TCAAGCAGTT 
ACCAAAGTTG CAAGTAGTGA TGGTAGTGTA CCAGGAAATA GCAGTGGGGA ACGTCTATCA 
GCGTTGTTAA AAAAATTGTT TGAAACGCAT GTTTCGAGCG ATGTTAATGA AGAGTTAGTA 
AAAACTGCTG CGAATGGTGA TGCAGTTAAA TGCGAAGAAT GTTTAAAGAG ACCCGAAGCT 
GATGTGAATG GCGTATTTTC GGGTCATACT GCTTTACAAG CGGCAAGTCA AAATGGTCAC 
CTAGAAGTCA TTAAAATTCT CCTCCGTTAT AAAGCAGATG TTGAAATTGA GGATAAAGAT 
GGAGATAGAG CTGTACATCA TGCAGCATTC GGAGATGAAC CGGGCGTAAT GGCATTATTA 
GCTGGTGCTG GAGCTGATTT GAATGCTAGA AATAAAAGAC GCCAAACTGC CCTCCACATC 
GCGGTTAATA AAGGACATGC CGGTGTAGTG CGCACGTTAT TGGAGTTGGG ATGCCACCCA 
AGTTTGCAGG ACTCCGAAGG TGACACTCCC CTTCACGATG CGATCTCTAA GAAACGGGAT 
GACATGTTGG CTCTGTTGTT GGACCATGCC GCAGACATCA CCCTCACGAA CAACAATGGC 
TTCAACGCTC TGCACCACGC TGCTCTTCGT GGAAACCCAA GGTATGTCCC CAAAATTTCG 
CTTCTCATTG GTTGCACTTC GGTGTTGGTA CGTATTTACT TGACGTATAA TATGCATCGT 
GTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020724090.1
CDS	94..2019
Translation	MEAGASTGTRTTRFMMEGVGARVIRGPEWKWGKQDGGEGHVGTVRNFESPEEVVVVWDNGTAANYRCSGAFDLRILDSAPTGVKHDGTMCDTCRQQPIFGIRWKCAECGNYDLCSICYHGDKHHLRHRFYRIATPGSERVLLEPRRKSKKIAIRGIFPGARVVRGVDWQWEDQDGGNGRRGKVNEIQDWSAASPRSAAYVIWDNGAKNLYRVGFEGMADLKVVNDAKGQTVYRYHLPLLGEQGPGRTGAHGLQIGDQVNVDLELEIVQSLQHGHGGWTDGMFECLGTTGTVVGIDEDHDIVVSYPSGNRWTFNPAVLTKVQIPVPVTSSSSDNQTFAVGDLVQICNDIEKIKRLQRGHGEWAEAMAPTMGKIGRVLQIYHDGDLKVEVCSTSWTYNPQAVTKVASSDGSVPGNSSGERLSALLKKLFETHVSSDVNEELVKTAANGDAVKCEECLKRPEADVNGVFSGHTALQAASQNGHLEVIKILLRYKADVEIEDKDGDRAVHHAAFGDEPGVMALLAGAGADLNARNKRRQTALHIAVNKGHAGVVRTLLELGCHPSLQDSEGDTPLHDAISKKRDDMLALLLDHAADITLTNNNGFNALHHAALRGNPRYVPKISLLIGCTSVLVRIYLTYNMHRV

Target ORF information:

RefSeq Version	XM_020868431.1
Organism	Bombus terrestris(buff-tailed bumblebee)
Definition	Bombus terrestris E3 ubiquitin-protein ligase MIB1 (LOC100643758), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020868431.1

ORF Insert Sequence:

ATGGAGGCTG GTGCGAGTAC CGGTACTCGT ACAACGCGAT TTATGATGGA GGGTGTCGGA 
GCCCGAGTCA TCCGTGGACC CGAATGGAAG TGGGGTAAAC AGGATGGAGG AGAAGGTCAT 
GTAGGAACTG TTAGAAATTT TGAATCTCCA GAAGAAGTAG TTGTTGTTTG GGATAATGGT 
ACAGCAGCTA ATTATCGATG TTCTGGTGCA TTTGATCTTC GAATTTTGGA TTCAGCTCCG 
ACTGGCGTAA AACATGATGG AACAATGTGT GATACGTGCA GACAACAACC AATTTTTGGA 
ATTCGTTGGA AATGTGCAGA ATGTGGGAAT TATGACCTTT GTTCTATTTG TTATCACGGA 
GACAAGCATC ACTTAAGACA TAGATTTTAT CGTATAGCAA CACCTGGAAG TGAAAGAGTA 
TTATTGGAGC CACGACGTAA AAGCAAAAAG ATTGCAATTC GTGGTATATT TCCTGGTGCC 
AGAGTTGTAA GAGGTGTTGA TTGGCAGTGG GAAGATCAAG ATGGTGGAAA TGGTCGAAGA 
GGTAAAGTTA ATGAAATTCA AGATTGGTCT GCAGCTAGTC CACGTTCGGC AGCCTATGTT 
ATATGGGATA ATGGTGCCAA AAATTTGTAT CGCGTTGGTT TTGAAGGAAT GGCGGATTTA 
AAAGTTGTTA ATGATGCTAA AGGCCAAACA GTTTACAGAT ATCATTTACC ACTATTAGGT 
GAACAGGGTC CAGGTCGCAC TGGCGCCCAT GGATTACAAA TTGGAGATCA GGTTAATGTT 
GATTTAGAAT TAGAAATCGT ACAATCGTTA CAACACGGGC ATGGTGGTTG GACGGATGGT 
ATGTTTGAGT GTCTTGGTAC TACAGGGACT GTTGTTGGTA TTGATGAAGA TCACGATATT 
GTTGTGTCCT ACCCAAGTGG AAATCGATGG ACCTTCAACC CTGCTGTATT GACAAAAGTA 
CAAATACCAG TCCCTGTTAC TAGTTCAAGT TCTGACAACC AAACATTTGC GGTTGGTGAT 
TTGGTACAAA TATGTAACGA TATAGAAAAA ATAAAACGAC TTCAACGAGG TCATGGTGAA 
TGGGCTGAAG CAATGGCACC GACTATGGGA AAGATTGGTA GAGTTTTGCA AATTTATCAC 
GATGGGGATT TAAAAGTAGA AGTTTGTAGT ACATCTTGGA CATATAATCC TCAAGCAGTT 
ACCAAAGTTG CAAGTAGTGA TGGTAGTGTA CCAGGAAATA GCAGTGGGGA ACGTCTATCA 
GCGTTGTTAA AAAAATTGTT TGAAACGCAT GTTTCGAGCG ATGTTAATGA AGAGTTAGTA 
AAAACTGCTG CGAATGGTGA TGCAGTTAAA TGCGAAGAAT GTTTAAAGAG ACCCGAAGCT 
GATGTGAATG GCGTATTTTC GGGTCATACT GCTTTACAAG CGGCAAGTCA AAATGGTCAC 
CTAGAAGTCA TTAAAATTCT CCTCCGTTAT AAAGCAGATG TTGAAATTGA GGATAAAGAT 
GGAGATAGAG CTGTACATCA TGCAGCATTC GGAGATGAAC CGGGCGTAAT GGCATTATTA 
GCTGGTGCTG GAGCTGATTT GAATGCTAGA AATAAAAGAC GCCAAACTGC CCTCCACATC 
GCGGTTAATA AAGGACATGC CGGTGTAGTG CGCACGTTAT TGGAGTTGGG ATGCCACCCA 
AGTTTGCAGG ACTCCGAAGG TGACACTCCC CTTCACGATG CGATCTCTAA GAAACGGGAT 
GACATGTTGG CTCTGTTGTT GGACCATGCC GCAGACATCA CCCTCACGAA CAACAATGGC 
TTCAACGCTC TGCACCACGC TGCTCTTCGT GGAAACCCAA GGTATGTCCC CAAAATTTCG 
CTTCTCATTG GTTGCACTTC GGTGTTGGTA CGTATTTACT TGACGTATAA TATGCATCGT 
GTATAG