GNE cDNA ORF clone, Bison bison bison

Gene
Clones
gRNAs

Summary

Gene Symbol	GNE
Entrez Gene ID	104992987
Full Name	glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase
Gene Type	protein-coding
Organism	Bison bison bison

mRNA and Protein(s)

mRNA	Protein	Name
XM_010846050.1	XP_010844352.1	bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase isoform X3
XM_010846048.1	XP_010844350.1	bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase isoform X1
XM_010846049.1	XP_010844351.1	bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase isoform X2

Pathways from BioSystems
Gene Ontology

The following GNE gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GNE cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBi69316	XM_010846048.1 Latest version!	Bison bison bison glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase (GNE), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OBi69317	XM_010846049.1 Latest version!	Bison bison bison glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase (GNE), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OBi69318	XM_010846050.1 Latest version!	Bison bison bison glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase (GNE), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GNE ( XM_010846048.1 ) cDNA ORF clone, Bison bison bison -> XP_010844350.1 Bison bison bison glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase (GNE), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBi69316
Clone ID Related Accession (Same CDS sequence)	XM_010846048.1
Accession Version	XM_010846048.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2259bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-12-30
Organism	Bison bison bison
Product	bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011494802.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bison bison bison Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAATTA ACGTGTATCC TCACCATGCC TTGGGCTTTC CAGGCCCCCA TGAACTCTAT 
TTTCAGAACC TCTCAAAAAG AAAACAAATC ATGGAGAAGA AAGAGAATAA CCGGAAGCTT 
CGGGTTTGTG TTGCTACTTG TAACCGTGCT GATTATTCTA AACTTGCCCC CATCATGTTT 
GGCATTAAAA TGGAACCTGA GTTCTTTGAA CTTGACGTGG TGGTACTTGG CTCTCATCTG 
ATAGATGACT ATGGAAACAC GTATCGCATG ATTGAACAAG ATGACTTTGA CATTAACACC 
AGGTTACACA CGATTGTCAG AGGGGAAGAT GAGGCAGCCA TGGTGGAGTC AGTAGGCCTG 
GCCCTTGTGA AGCTACCAGA TGTCCTTAAT CGCCTGAAAC CTGATATCAT GATTGTTCAT 
GGAGACAGGT TTGACGCCCT GGCTCTGGCT ACATCTGCTG CCTTGATGAA CATCCGAATC 
CTTCACATTG AAGGGGGGGA AGTCAGTGGG ACCATTGATG ACTCTATCAG ACACGCCATA 
ACAAAACTGG CTCATTATCA CGTGTGCTGC ACCCGAAGTG CAGAGCAACA CCTGATATCC 
ATGTGTGAGG ACCATGACCG CATCCTGCTG GCAGGCTGCC CTTCCTACGA CAAGCTGCTC 
TCCGCCAAGA ACAAAGACTA CATGAGCATC ATTCGGATGT GGTTAGGTGA TGATGTAAAA 
CCTAAAGATT ACATTGTCGC ACTTCAGCAC CCTGTGACCA CTGACATTAA GCATTCTATA 
AAAATGTTTG AATTAACATT GGATGCACTT ATCTCATTTA ACAAGCGGAC CCTAGTTCTG 
TTTCCAAATA TTGATGCAGG AAGCAAAGAG ATGGTTCGAG TGATGCGGAA GAAGGGCATT 
GAGCATCATC CCAATTTTCG TGCAGTTAAA CATGTCCCAT TTGACCAGTT TATACAGCTG 
GTTGCCCATG CTGGTTGTAT GATTGGGAAC AGCAGCTGTG GGGTACGAGA AGTTGGAGCT 
TTTGGAACAC CTGTGATCAA CTTAGGAACA CGTCAGATTG GAAGAGAAAC AGGGGAGAAT 
GTCCTTCATG TCCGGGATGC TGATACCCAA GACAAAATAT TGCAAGCGTT GCACCTTCAG 
TTTGGTAAAC AGTACCCTTG TTCAAAGATA TATGGGGATG GAAATGCTGT TCCAAGGATT 
TTGAAGTTTC TCAAATCTAT TGATCTTCAA GAGCCACTAC AAAAGAAATT TTGTTTTCCT 
CCTGTGAAGG ATAACATCTC TCAAGATATT GACCATATTC TTGAAACTCT AAGTGCCTTG 
GCTGTTGATC TTGGCGGGAC GAACCTCCGA GTTGCAATAG TCAGCATGAA GGGTGAAATA 
GTTAAGAAGT ATACTCAGTT CAATCCTAAA ACGTATGAAG AGAGGATTAA TTTAATCCTA 
CAGATGTGTG TAGAAGCCGC AGCAGAAGCT GTAAAACTGA ACTGCAGAAT TTTGGGAGTA 
GGCATTTCTA CAGGTGGCCG TGTAAATCCT CGGGAAGGAA TCGTGCTGCA TTCAACCAAA 
CTGATTCAAG AATGGAACTC CGTGGACCTC AGAAGCCCCC TGTCTGACAC TTTGCATCTC 
CCCGTGTGGG TAGACAATGA TGGCAACTGT GCTGCCCTGG CAGAAAGGAA ATTTGGCCAA 
GGAAAAGGCC TTGAAAACTT TGTGACACTT ATCACAGGCA CAGGGATCGG CGGGGGGATC 
GTCCACCAGC ATGAACTGAT CCACGGCAGC TCCTTCTGTG CTGCAGAGCT TGGCCACATT 
GTGGTGTCCC TGGATGGGCC TGATTGTTCC TGTGGAAGCC ACGGGTGCAT TGAAGCATAT 
GCCTCTGGAA TGGCCTTGCA GAGGGAAGCA AAAAAGCTAC ATGATGAAGA CCAGCTCTTG 
GTGGAAGGGA TGTCAGTGCC AAAAGATGAA GCTGTGGGCG CACTCCATCT CATCCAGGCT 
GCAAAACTTG GCAACGCAAA GGCCCAGAGC ATCTTGAGAA CAGCTGGAAC AGCTTTAGGT 
CTTGGGGTTG TGAACATCCT CCACACTATG AATCCTTCCC TTGTGATCCT CTCTGGAATC 
CTAGCCAGTC ACTACATCCA CACTGTCAAA GACGTCATCC GCCAGCAAGC CTTGTCCTCG 
GTTCAGGATG TGGACGTGGT GGTTTCCGAT TTGGTGGAAC CTGCTCTGCT CGGCGCCGCC 
AGCATGGTCC TGGACTACAC GACTCGCAGG ATCTACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010844350.1
CDS	104..2362
Translation	MEINVYPHHALGFPGPHELYFQNLSKRKQIMEKKENNRKLRVCVATCNRADYSKLAPIMFGIKMEPEFFELDVVVLGSHLIDDYGNTYRMIEQDDFDINTRLHTIVRGEDEAAMVESVGLALVKLPDVLNRLKPDIMIVHGDRFDALALATSAALMNIRILHIEGGEVSGTIDDSIRHAITKLAHYHVCCTRSAEQHLISMCEDHDRILLAGCPSYDKLLSAKNKDYMSIIRMWLGDDVKPKDYIVALQHPVTTDIKHSIKMFELTLDALISFNKRTLVLFPNIDAGSKEMVRVMRKKGIEHHPNFRAVKHVPFDQFIQLVAHAGCMIGNSSCGVREVGAFGTPVINLGTRQIGRETGENVLHVRDADTQDKILQALHLQFGKQYPCSKIYGDGNAVPRILKFLKSIDLQEPLQKKFCFPPVKDNISQDIDHILETLSALAVDLGGTNLRVAIVSMKGEIVKKYTQFNPKTYEERINLILQMCVEAAAEAVKLNCRILGVGISTGGRVNPREGIVLHSTKLIQEWNSVDLRSPLSDTLHLPVWVDNDGNCAALAERKFGQGKGLENFVTLITGTGIGGGIVHQHELIHGSSFCAAELGHIVVSLDGPDCSCGSHGCIEAYASGMALQREAKKLHDEDQLLVEGMSVPKDEAVGALHLIQAAKLGNAKAQSILRTAGTALGLGVVNILHTMNPSLVILSGILASHYIHTVKDVIRQQALSSVQDVDVVVSDLVEPALLGAASMVLDYTTRRIY

Target ORF information:

RefSeq Version	XM_010846048.1
Organism	Bison bison bison
Definition	Bison bison bison glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase (GNE), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010846048.1

ORF Insert Sequence:

ATGGAAATTA ACGTGTATCC TCACCATGCC TTGGGCTTTC CAGGCCCCCA TGAACTCTAT 
TTTCAGAACC TCTCAAAAAG AAAACAAATC ATGGAGAAGA AAGAGAATAA CCGGAAGCTT 
CGGGTTTGTG TTGCTACTTG TAACCGTGCT GATTATTCTA AACTTGCCCC CATCATGTTT 
GGCATTAAAA TGGAACCTGA GTTCTTTGAA CTTGACGTGG TGGTACTTGG CTCTCATCTG 
ATAGATGACT ATGGAAACAC GTATCGCATG ATTGAACAAG ATGACTTTGA CATTAACACC 
AGGTTACACA CGATTGTCAG AGGGGAAGAT GAGGCAGCCA TGGTGGAGTC AGTAGGCCTG 
GCCCTTGTGA AGCTACCAGA TGTCCTTAAT CGCCTGAAAC CTGATATCAT GATTGTTCAT 
GGAGACAGGT TTGACGCCCT GGCTCTGGCT ACATCTGCTG CCTTGATGAA CATCCGAATC 
CTTCACATTG AAGGGGGGGA AGTCAGTGGG ACCATTGATG ACTCTATCAG ACACGCCATA 
ACAAAACTGG CTCATTATCA CGTGTGCTGC ACCCGAAGTG CAGAGCAACA CCTGATATCC 
ATGTGTGAGG ACCATGACCG CATCCTGCTG GCAGGCTGCC CTTCCTACGA CAAGCTGCTC 
TCCGCCAAGA ACAAAGACTA CATGAGCATC ATTCGGATGT GGTTAGGTGA TGATGTAAAA 
CCTAAAGATT ACATTGTCGC ACTTCAGCAC CCTGTGACCA CTGACATTAA GCATTCTATA 
AAAATGTTTG AATTAACATT GGATGCACTT ATCTCATTTA ACAAGCGGAC CCTAGTTCTG 
TTTCCAAATA TTGATGCAGG AAGCAAAGAG ATGGTTCGAG TGATGCGGAA GAAGGGCATT 
GAGCATCATC CCAATTTTCG TGCAGTTAAA CATGTCCCAT TTGACCAGTT TATACAGCTG 
GTTGCCCATG CTGGTTGTAT GATTGGGAAC AGCAGCTGTG GGGTACGAGA AGTTGGAGCT 
TTTGGAACAC CTGTGATCAA CTTAGGAACA CGTCAGATTG GAAGAGAAAC AGGGGAGAAT 
GTCCTTCATG TCCGGGATGC TGATACCCAA GACAAAATAT TGCAAGCGTT GCACCTTCAG 
TTTGGTAAAC AGTACCCTTG TTCAAAGATA TATGGGGATG GAAATGCTGT TCCAAGGATT 
TTGAAGTTTC TCAAATCTAT TGATCTTCAA GAGCCACTAC AAAAGAAATT TTGTTTTCCT 
CCTGTGAAGG ATAACATCTC TCAAGATATT GACCATATTC TTGAAACTCT AAGTGCCTTG 
GCTGTTGATC TTGGCGGGAC GAACCTCCGA GTTGCAATAG TCAGCATGAA GGGTGAAATA 
GTTAAGAAGT ATACTCAGTT CAATCCTAAA ACGTATGAAG AGAGGATTAA TTTAATCCTA 
CAGATGTGTG TAGAAGCCGC AGCAGAAGCT GTAAAACTGA ACTGCAGAAT TTTGGGAGTA 
GGCATTTCTA CAGGTGGCCG TGTAAATCCT CGGGAAGGAA TCGTGCTGCA TTCAACCAAA 
CTGATTCAAG AATGGAACTC CGTGGACCTC AGAAGCCCCC TGTCTGACAC TTTGCATCTC 
CCCGTGTGGG TAGACAATGA TGGCAACTGT GCTGCCCTGG CAGAAAGGAA ATTTGGCCAA 
GGAAAAGGCC TTGAAAACTT TGTGACACTT ATCACAGGCA CAGGGATCGG CGGGGGGATC 
GTCCACCAGC ATGAACTGAT CCACGGCAGC TCCTTCTGTG CTGCAGAGCT TGGCCACATT 
GTGGTGTCCC TGGATGGGCC TGATTGTTCC TGTGGAAGCC ACGGGTGCAT TGAAGCATAT 
GCCTCTGGAA TGGCCTTGCA GAGGGAAGCA AAAAAGCTAC ATGATGAAGA CCAGCTCTTG 
GTGGAAGGGA TGTCAGTGCC AAAAGATGAA GCTGTGGGCG CACTCCATCT CATCCAGGCT 
GCAAAACTTG GCAACGCAAA GGCCCAGAGC ATCTTGAGAA CAGCTGGAAC AGCTTTAGGT 
CTTGGGGTTG TGAACATCCT CCACACTATG AATCCTTCCC TTGTGATCCT CTCTGGAATC 
CTAGCCAGTC ACTACATCCA CACTGTCAAA GACGTCATCC GCCAGCAAGC CTTGTCCTCG 
GTTCAGGATG TGGACGTGGT GGTTTCCGAT TTGGTGGAAC CTGCTCTGCT CGGCGCCGCC 
AGCATGGTCC TGGACTACAC GACTCGCAGG ATCTACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GNE ( XM_010846049.1 ) cDNA ORF clone, Bison bison bison -> XP_010844351.1 Bison bison bison glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase (GNE), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBi69317
Clone ID Related Accession (Same CDS sequence)	XM_010846049.1
Accession Version	XM_010846049.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2169bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-12-30
Organism	Bison bison bison
Product	bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011494802.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bison bison bison Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAAGA AAGAGAATAA CCGGAAGCTT CGGGTTTGTG TTGCTACTTG TAACCGTGCT 
GATTATTCTA AACTTGCCCC CATCATGTTT GGCATTAAAA TGGAACCTGA GTTCTTTGAA 
CTTGACGTGG TGGTACTTGG CTCTCATCTG ATAGATGACT ATGGAAACAC GTATCGCATG 
ATTGAACAAG ATGACTTTGA CATTAACACC AGGTTACACA CGATTGTCAG AGGGGAAGAT 
GAGGCAGCCA TGGTGGAGTC AGTAGGCCTG GCCCTTGTGA AGCTACCAGA TGTCCTTAAT 
CGCCTGAAAC CTGATATCAT GATTGTTCAT GGAGACAGGT TTGACGCCCT GGCTCTGGCT 
ACATCTGCTG CCTTGATGAA CATCCGAATC CTTCACATTG AAGGGGGGGA AGTCAGTGGG 
ACCATTGATG ACTCTATCAG ACACGCCATA ACAAAACTGG CTCATTATCA CGTGTGCTGC 
ACCCGAAGTG CAGAGCAACA CCTGATATCC ATGTGTGAGG ACCATGACCG CATCCTGCTG 
GCAGGCTGCC CTTCCTACGA CAAGCTGCTC TCCGCCAAGA ACAAAGACTA CATGAGCATC 
ATTCGGATGT GGTTAGGTGA TGATGTAAAA CCTAAAGATT ACATTGTCGC ACTTCAGCAC 
CCTGTGACCA CTGACATTAA GCATTCTATA AAAATGTTTG AATTAACATT GGATGCACTT 
ATCTCATTTA ACAAGCGGAC CCTAGTTCTG TTTCCAAATA TTGATGCAGG AAGCAAAGAG 
ATGGTTCGAG TGATGCGGAA GAAGGGCATT GAGCATCATC CCAATTTTCG TGCAGTTAAA 
CATGTCCCAT TTGACCAGTT TATACAGCTG GTTGCCCATG CTGGTTGTAT GATTGGGAAC 
AGCAGCTGTG GGGTACGAGA AGTTGGAGCT TTTGGAACAC CTGTGATCAA CTTAGGAACA 
CGTCAGATTG GAAGAGAAAC AGGGGAGAAT GTCCTTCATG TCCGGGATGC TGATACCCAA 
GACAAAATAT TGCAAGCGTT GCACCTTCAG TTTGGTAAAC AGTACCCTTG TTCAAAGATA 
TATGGGGATG GAAATGCTGT TCCAAGGATT TTGAAGTTTC TCAAATCTAT TGATCTTCAA 
GAGCCACTAC AAAAGAAATT TTGTTTTCCT CCTGTGAAGG ATAACATCTC TCAAGATATT 
GACCATATTC TTGAAACTCT AAGTGCCTTG GCTGTTGATC TTGGCGGGAC GAACCTCCGA 
GTTGCAATAG TCAGCATGAA GGGTGAAATA GTTAAGAAGT ATACTCAGTT CAATCCTAAA 
ACGTATGAAG AGAGGATTAA TTTAATCCTA CAGATGTGTG TAGAAGCCGC AGCAGAAGCT 
GTAAAACTGA ACTGCAGAAT TTTGGGAGTA GGCATTTCTA CAGGTGGCCG TGTAAATCCT 
CGGGAAGGAA TCGTGCTGCA TTCAACCAAA CTGATTCAAG AATGGAACTC CGTGGACCTC 
AGAAGCCCCC TGTCTGACAC TTTGCATCTC CCCGTGTGGG TAGACAATGA TGGCAACTGT 
GCTGCCCTGG CAGAAAGGAA ATTTGGCCAA GGAAAAGGCC TTGAAAACTT TGTGACACTT 
ATCACAGGCA CAGGGATCGG CGGGGGGATC GTCCACCAGC ATGAACTGAT CCACGGCAGC 
TCCTTCTGTG CTGCAGAGCT TGGCCACATT GTGGTGTCCC TGGATGGGCC TGATTGTTCC 
TGTGGAAGCC ACGGGTGCAT TGAAGCATAT GCCTCTGGAA TGGCCTTGCA GAGGGAAGCA 
AAAAAGCTAC ATGATGAAGA CCAGCTCTTG GTGGAAGGGA TGTCAGTGCC AAAAGATGAA 
GCTGTGGGCG CACTCCATCT CATCCAGGCT GCAAAACTTG GCAACGCAAA GGCCCAGAGC 
ATCTTGAGAA CAGCTGGAAC AGCTTTAGGT CTTGGGGTTG TGAACATCCT CCACACTATG 
AATCCTTCCC TTGTGATCCT CTCTGGAATC CTAGCCAGTC ACTACATCCA CACTGTCAAA 
GACGTCATCC GCCAGCAAGC CTTGTCCTCG GTTCAGGATG TGGACGTGGT GGTTTCCGAT 
TTGGTGGAAC CTGCTCTGCT CGGCGCCGCC AGCATGGTCC TGGACTACAC GACTCGCAGG 
ATCTACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010844351.1
CDS	101..2269
Translation	MEKKENNRKLRVCVATCNRADYSKLAPIMFGIKMEPEFFELDVVVLGSHLIDDYGNTYRMIEQDDFDINTRLHTIVRGEDEAAMVESVGLALVKLPDVLNRLKPDIMIVHGDRFDALALATSAALMNIRILHIEGGEVSGTIDDSIRHAITKLAHYHVCCTRSAEQHLISMCEDHDRILLAGCPSYDKLLSAKNKDYMSIIRMWLGDDVKPKDYIVALQHPVTTDIKHSIKMFELTLDALISFNKRTLVLFPNIDAGSKEMVRVMRKKGIEHHPNFRAVKHVPFDQFIQLVAHAGCMIGNSSCGVREVGAFGTPVINLGTRQIGRETGENVLHVRDADTQDKILQALHLQFGKQYPCSKIYGDGNAVPRILKFLKSIDLQEPLQKKFCFPPVKDNISQDIDHILETLSALAVDLGGTNLRVAIVSMKGEIVKKYTQFNPKTYEERINLILQMCVEAAAEAVKLNCRILGVGISTGGRVNPREGIVLHSTKLIQEWNSVDLRSPLSDTLHLPVWVDNDGNCAALAERKFGQGKGLENFVTLITGTGIGGGIVHQHELIHGSSFCAAELGHIVVSLDGPDCSCGSHGCIEAYASGMALQREAKKLHDEDQLLVEGMSVPKDEAVGALHLIQAAKLGNAKAQSILRTAGTALGLGVVNILHTMNPSLVILSGILASHYIHTVKDVIRQQALSSVQDVDVVVSDLVEPALLGAASMVLDYTTRRIY

Target ORF information:

RefSeq Version	XM_010846049.1
Organism	Bison bison bison
Definition	Bison bison bison glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase (GNE), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010846049.1

ORF Insert Sequence:

ATGGAGAAGA AAGAGAATAA CCGGAAGCTT CGGGTTTGTG TTGCTACTTG TAACCGTGCT 
GATTATTCTA AACTTGCCCC CATCATGTTT GGCATTAAAA TGGAACCTGA GTTCTTTGAA 
CTTGACGTGG TGGTACTTGG CTCTCATCTG ATAGATGACT ATGGAAACAC GTATCGCATG 
ATTGAACAAG ATGACTTTGA CATTAACACC AGGTTACACA CGATTGTCAG AGGGGAAGAT 
GAGGCAGCCA TGGTGGAGTC AGTAGGCCTG GCCCTTGTGA AGCTACCAGA TGTCCTTAAT 
CGCCTGAAAC CTGATATCAT GATTGTTCAT GGAGACAGGT TTGACGCCCT GGCTCTGGCT 
ACATCTGCTG CCTTGATGAA CATCCGAATC CTTCACATTG AAGGGGGGGA AGTCAGTGGG 
ACCATTGATG ACTCTATCAG ACACGCCATA ACAAAACTGG CTCATTATCA CGTGTGCTGC 
ACCCGAAGTG CAGAGCAACA CCTGATATCC ATGTGTGAGG ACCATGACCG CATCCTGCTG 
GCAGGCTGCC CTTCCTACGA CAAGCTGCTC TCCGCCAAGA ACAAAGACTA CATGAGCATC 
ATTCGGATGT GGTTAGGTGA TGATGTAAAA CCTAAAGATT ACATTGTCGC ACTTCAGCAC 
CCTGTGACCA CTGACATTAA GCATTCTATA AAAATGTTTG AATTAACATT GGATGCACTT 
ATCTCATTTA ACAAGCGGAC CCTAGTTCTG TTTCCAAATA TTGATGCAGG AAGCAAAGAG 
ATGGTTCGAG TGATGCGGAA GAAGGGCATT GAGCATCATC CCAATTTTCG TGCAGTTAAA 
CATGTCCCAT TTGACCAGTT TATACAGCTG GTTGCCCATG CTGGTTGTAT GATTGGGAAC 
AGCAGCTGTG GGGTACGAGA AGTTGGAGCT TTTGGAACAC CTGTGATCAA CTTAGGAACA 
CGTCAGATTG GAAGAGAAAC AGGGGAGAAT GTCCTTCATG TCCGGGATGC TGATACCCAA 
GACAAAATAT TGCAAGCGTT GCACCTTCAG TTTGGTAAAC AGTACCCTTG TTCAAAGATA 
TATGGGGATG GAAATGCTGT TCCAAGGATT TTGAAGTTTC TCAAATCTAT TGATCTTCAA 
GAGCCACTAC AAAAGAAATT TTGTTTTCCT CCTGTGAAGG ATAACATCTC TCAAGATATT 
GACCATATTC TTGAAACTCT AAGTGCCTTG GCTGTTGATC TTGGCGGGAC GAACCTCCGA 
GTTGCAATAG TCAGCATGAA GGGTGAAATA GTTAAGAAGT ATACTCAGTT CAATCCTAAA 
ACGTATGAAG AGAGGATTAA TTTAATCCTA CAGATGTGTG TAGAAGCCGC AGCAGAAGCT 
GTAAAACTGA ACTGCAGAAT TTTGGGAGTA GGCATTTCTA CAGGTGGCCG TGTAAATCCT 
CGGGAAGGAA TCGTGCTGCA TTCAACCAAA CTGATTCAAG AATGGAACTC CGTGGACCTC 
AGAAGCCCCC TGTCTGACAC TTTGCATCTC CCCGTGTGGG TAGACAATGA TGGCAACTGT 
GCTGCCCTGG CAGAAAGGAA ATTTGGCCAA GGAAAAGGCC TTGAAAACTT TGTGACACTT 
ATCACAGGCA CAGGGATCGG CGGGGGGATC GTCCACCAGC ATGAACTGAT CCACGGCAGC 
TCCTTCTGTG CTGCAGAGCT TGGCCACATT GTGGTGTCCC TGGATGGGCC TGATTGTTCC 
TGTGGAAGCC ACGGGTGCAT TGAAGCATAT GCCTCTGGAA TGGCCTTGCA GAGGGAAGCA 
AAAAAGCTAC ATGATGAAGA CCAGCTCTTG GTGGAAGGGA TGTCAGTGCC AAAAGATGAA 
GCTGTGGGCG CACTCCATCT CATCCAGGCT GCAAAACTTG GCAACGCAAA GGCCCAGAGC 
ATCTTGAGAA CAGCTGGAAC AGCTTTAGGT CTTGGGGTTG TGAACATCCT CCACACTATG 
AATCCTTCCC TTGTGATCCT CTCTGGAATC CTAGCCAGTC ACTACATCCA CACTGTCAAA 
GACGTCATCC GCCAGCAAGC CTTGTCCTCG GTTCAGGATG TGGACGTGGT GGTTTCCGAT 
TTGGTGGAAC CTGCTCTGCT CGGCGCCGCC AGCATGGTCC TGGACTACAC GACTCGCAGG 
ATCTACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GNE ( XM_010846050.1 ) cDNA ORF clone, Bison bison bison -> XP_010844352.1 Bison bison bison glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase (GNE), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBi69318
Clone ID Related Accession (Same CDS sequence)	XM_010846050.1
Accession Version	XM_010846050.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1992bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-12-30
Organism	Bison bison bison
Product	bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011494802.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bison bison bison Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTGAAC AAGATGACTT TGACATTAAC ACCAGGTTAC ACACGATTGT CAGAGGGGAA 
GATGAGGCAG CCATGGTGGA GTCAGTAGGC CTGGCCCTTG TGAAGCTACC AGATGTCCTT 
AATCGCCTGA AACCTGATAT CATGATTGTT CATGGAGACA GGTTTGACGC CCTGGCTCTG 
GCTACATCTG CTGCCTTGAT GAACATCCGA ATCCTTCACA TTGAAGGGGG GGAAGTCAGT 
GGGACCATTG ATGACTCTAT CAGACACGCC ATAACAAAAC TGGCTCATTA TCACGTGTGC 
TGCACCCGAA GTGCAGAGCA ACACCTGATA TCCATGTGTG AGGACCATGA CCGCATCCTG 
CTGGCAGGCT GCCCTTCCTA CGACAAGCTG CTCTCCGCCA AGAACAAAGA CTACATGAGC 
ATCATTCGGA TGTGGTTAGG TGATGATGTA AAACCTAAAG ATTACATTGT CGCACTTCAG 
CACCCTGTGA CCACTGACAT TAAGCATTCT ATAAAAATGT TTGAATTAAC ATTGGATGCA 
CTTATCTCAT TTAACAAGCG GACCCTAGTT CTGTTTCCAA ATATTGATGC AGGAAGCAAA 
GAGATGGTTC GAGTGATGCG GAAGAAGGGC ATTGAGCATC ATCCCAATTT TCGTGCAGTT 
AAACATGTCC CATTTGACCA GTTTATACAG CTGGTTGCCC ATGCTGGTTG TATGATTGGG 
AACAGCAGCT GTGGGGTACG AGAAGTTGGA GCTTTTGGAA CACCTGTGAT CAACTTAGGA 
ACACGTCAGA TTGGAAGAGA AACAGGGGAG AATGTCCTTC ATGTCCGGGA TGCTGATACC 
CAAGACAAAA TATTGCAAGC GTTGCACCTT CAGTTTGGTA AACAGTACCC TTGTTCAAAG 
ATATATGGGG ATGGAAATGC TGTTCCAAGG ATTTTGAAGT TTCTCAAATC TATTGATCTT 
CAAGAGCCAC TACAAAAGAA ATTTTGTTTT CCTCCTGTGA AGGATAACAT CTCTCAAGAT 
ATTGACCATA TTCTTGAAAC TCTAAGTGCC TTGGCTGTTG ATCTTGGCGG GACGAACCTC 
CGAGTTGCAA TAGTCAGCAT GAAGGGTGAA ATAGTTAAGA AGTATACTCA GTTCAATCCT 
AAAACGTATG AAGAGAGGAT TAATTTAATC CTACAGATGT GTGTAGAAGC CGCAGCAGAA 
GCTGTAAAAC TGAACTGCAG AATTTTGGGA GTAGGCATTT CTACAGGTGG CCGTGTAAAT 
CCTCGGGAAG GAATCGTGCT GCATTCAACC AAACTGATTC AAGAATGGAA CTCCGTGGAC 
CTCAGAAGCC CCCTGTCTGA CACTTTGCAT CTCCCCGTGT GGGTAGACAA TGATGGCAAC 
TGTGCTGCCC TGGCAGAAAG GAAATTTGGC CAAGGAAAAG GCCTTGAAAA CTTTGTGACA 
CTTATCACAG GCACAGGGAT CGGCGGGGGG ATCGTCCACC AGCATGAACT GATCCACGGC 
AGCTCCTTCT GTGCTGCAGA GCTTGGCCAC ATTGTGGTGT CCCTGGATGG GCCTGATTGT 
TCCTGTGGAA GCCACGGGTG CATTGAAGCA TATGCCTCTG GAATGGCCTT GCAGAGGGAA 
GCAAAAAAGC TACATGATGA AGACCAGCTC TTGGTGGAAG GGATGTCAGT GCCAAAAGAT 
GAAGCTGTGG GCGCACTCCA TCTCATCCAG GCTGCAAAAC TTGGCAACGC AAAGGCCCAG 
AGCATCTTGA GAACAGCTGG AACAGCTTTA GGTCTTGGGG TTGTGAACAT CCTCCACACT 
ATGAATCCTT CCCTTGTGAT CCTCTCTGGA ATCCTAGCCA GTCACTACAT CCACACTGTC 
AAAGACGTCA TCCGCCAGCA AGCCTTGTCC TCGGTTCAGG ATGTGGACGT GGTGGTTTCC 
GATTTGGTGG AACCTGCTCT GCTCGGCGCC GCCAGCATGG TCCTGGACTA CACGACTCGC 
AGGATCTACT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010844352.1
CDS	81..2072
Translation	MIEQDDFDINTRLHTIVRGEDEAAMVESVGLALVKLPDVLNRLKPDIMIVHGDRFDALALATSAALMNIRILHIEGGEVSGTIDDSIRHAITKLAHYHVCCTRSAEQHLISMCEDHDRILLAGCPSYDKLLSAKNKDYMSIIRMWLGDDVKPKDYIVALQHPVTTDIKHSIKMFELTLDALISFNKRTLVLFPNIDAGSKEMVRVMRKKGIEHHPNFRAVKHVPFDQFIQLVAHAGCMIGNSSCGVREVGAFGTPVINLGTRQIGRETGENVLHVRDADTQDKILQALHLQFGKQYPCSKIYGDGNAVPRILKFLKSIDLQEPLQKKFCFPPVKDNISQDIDHILETLSALAVDLGGTNLRVAIVSMKGEIVKKYTQFNPKTYEERINLILQMCVEAAAEAVKLNCRILGVGISTGGRVNPREGIVLHSTKLIQEWNSVDLRSPLSDTLHLPVWVDNDGNCAALAERKFGQGKGLENFVTLITGTGIGGGIVHQHELIHGSSFCAAELGHIVVSLDGPDCSCGSHGCIEAYASGMALQREAKKLHDEDQLLVEGMSVPKDEAVGALHLIQAAKLGNAKAQSILRTAGTALGLGVVNILHTMNPSLVILSGILASHYIHTVKDVIRQQALSSVQDVDVVVSDLVEPALLGAASMVLDYTTRRIY

Target ORF information:

RefSeq Version	XM_010846050.1
Organism	Bison bison bison
Definition	Bison bison bison glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase (GNE), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010846050.1

ORF Insert Sequence:

ATGATTGAAC AAGATGACTT TGACATTAAC ACCAGGTTAC ACACGATTGT CAGAGGGGAA 
GATGAGGCAG CCATGGTGGA GTCAGTAGGC CTGGCCCTTG TGAAGCTACC AGATGTCCTT 
AATCGCCTGA AACCTGATAT CATGATTGTT CATGGAGACA GGTTTGACGC CCTGGCTCTG 
GCTACATCTG CTGCCTTGAT GAACATCCGA ATCCTTCACA TTGAAGGGGG GGAAGTCAGT 
GGGACCATTG ATGACTCTAT CAGACACGCC ATAACAAAAC TGGCTCATTA TCACGTGTGC 
TGCACCCGAA GTGCAGAGCA ACACCTGATA TCCATGTGTG AGGACCATGA CCGCATCCTG 
CTGGCAGGCT GCCCTTCCTA CGACAAGCTG CTCTCCGCCA AGAACAAAGA CTACATGAGC 
ATCATTCGGA TGTGGTTAGG TGATGATGTA AAACCTAAAG ATTACATTGT CGCACTTCAG 
CACCCTGTGA CCACTGACAT TAAGCATTCT ATAAAAATGT TTGAATTAAC ATTGGATGCA 
CTTATCTCAT TTAACAAGCG GACCCTAGTT CTGTTTCCAA ATATTGATGC AGGAAGCAAA 
GAGATGGTTC GAGTGATGCG GAAGAAGGGC ATTGAGCATC ATCCCAATTT TCGTGCAGTT 
AAACATGTCC CATTTGACCA GTTTATACAG CTGGTTGCCC ATGCTGGTTG TATGATTGGG 
AACAGCAGCT GTGGGGTACG AGAAGTTGGA GCTTTTGGAA CACCTGTGAT CAACTTAGGA 
ACACGTCAGA TTGGAAGAGA AACAGGGGAG AATGTCCTTC ATGTCCGGGA TGCTGATACC 
CAAGACAAAA TATTGCAAGC GTTGCACCTT CAGTTTGGTA AACAGTACCC TTGTTCAAAG 
ATATATGGGG ATGGAAATGC TGTTCCAAGG ATTTTGAAGT TTCTCAAATC TATTGATCTT 
CAAGAGCCAC TACAAAAGAA ATTTTGTTTT CCTCCTGTGA AGGATAACAT CTCTCAAGAT 
ATTGACCATA TTCTTGAAAC TCTAAGTGCC TTGGCTGTTG ATCTTGGCGG GACGAACCTC 
CGAGTTGCAA TAGTCAGCAT GAAGGGTGAA ATAGTTAAGA AGTATACTCA GTTCAATCCT 
AAAACGTATG AAGAGAGGAT TAATTTAATC CTACAGATGT GTGTAGAAGC CGCAGCAGAA 
GCTGTAAAAC TGAACTGCAG AATTTTGGGA GTAGGCATTT CTACAGGTGG CCGTGTAAAT 
CCTCGGGAAG GAATCGTGCT GCATTCAACC AAACTGATTC AAGAATGGAA CTCCGTGGAC 
CTCAGAAGCC CCCTGTCTGA CACTTTGCAT CTCCCCGTGT GGGTAGACAA TGATGGCAAC 
TGTGCTGCCC TGGCAGAAAG GAAATTTGGC CAAGGAAAAG GCCTTGAAAA CTTTGTGACA 
CTTATCACAG GCACAGGGAT CGGCGGGGGG ATCGTCCACC AGCATGAACT GATCCACGGC 
AGCTCCTTCT GTGCTGCAGA GCTTGGCCAC ATTGTGGTGT CCCTGGATGG GCCTGATTGT 
TCCTGTGGAA GCCACGGGTG CATTGAAGCA TATGCCTCTG GAATGGCCTT GCAGAGGGAA 
GCAAAAAAGC TACATGATGA AGACCAGCTC TTGGTGGAAG GGATGTCAGT GCCAAAAGAT 
GAAGCTGTGG GCGCACTCCA TCTCATCCAG GCTGCAAAAC TTGGCAACGC AAAGGCCCAG 
AGCATCTTGA GAACAGCTGG AACAGCTTTA GGTCTTGGGG TTGTGAACAT CCTCCACACT 
ATGAATCCTT CCCTTGTGAT CCTCTCTGGA ATCCTAGCCA GTCACTACAT CCACACTGTC 
AAAGACGTCA TCCGCCAGCA AGCCTTGTCC TCGGTTCAGG ATGTGGACGT GGTGGTTTCC 
GATTTGGTGG AACCTGCTCT GCTCGGCGCC GCCAGCATGG TCCTGGACTA CACGACTCGC 
AGGATCTACT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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見積依頼

Eメール

[email protected]

電話

03-6811-6572

FAX

03-6811-6573
050-3174-7589(受注専用)

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オンライン注文

GNE cDNA ORF clone, Bison bison bison

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム