HSD11B1 cDNA ORF clone, Bison bison bison

Gene
Clones
gRNAs

Summary

Gene Symbol	HSD11B1
Entrez Gene ID	104986331
Full Name	hydroxysteroid 11-beta dehydrogenase 1
Gene Type	protein-coding
Organism	Bison bison bison

mRNA and Protein(s)

mRNA	Protein	Name
XM_010836863.1	XP_010835165.1	corticosteroid 11-beta-dehydrogenase isozyme 1

Pathways from BioSystems
Gene Ontology

The following HSD11B1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HSD11B1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBi62454	XM_010836863.1 Latest version!	Bison bison bison hydroxysteroid (11-beta) dehydrogenase 1 (HSD11B1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$174.30 $249.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

HSD11B1 ( XM_010836863.1 ) cDNA ORF clone, Bison bison bison -> XP_010835165.1 Bison bison bison hydroxysteroid (11-beta) dehydrogenase 1 (HSD11B1), mRNA.

Price & Availability↑

CloneID	OBi62454
Clone ID Related Accession (Same CDS sequence)	XM_010836863.1
Accession Version	XM_010836863.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 879bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-12-30
Organism	Bison bison bison
Product	corticosteroid 11-beta-dehydrogenase isozyme 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011494639.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bison bison bison Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTTTA TGAAAAAATA CCTCCTCCCC ATCCTGGGGA TCTTCTTGGC CTACTACTAC 
TATTCTGCGA ATGAGGAATT CAGACCAGAG ATGCTTCGAG GAAAGAGAGT GATTGTCACG 
GGGGCCAGCA AGGGAATCGG AAGAGAAATG GCCTATCACC TGGCAAGGAT GGGAGCACAT 
GTGGTAGTGA CAGCGAGGTC AGAAGAAAGT CTAAAGAAGG TGGTATCCCG CTGCCTGGAG 
CTGGGGGCGG CCTCAGCACA CTACGTGGCG GGCACCATGG AGAACATGAC CTTCGCAGAA 
CAATTTGTTG CCAAAGCTGG AGAGCTCGTG GGGGGACTTG ACATGCTCAT TCTCAACCAC 
ATCCACTACA CGCCTTTGGG GGTGTTTAGC AATGACATCC ATCTCTTGCG CAGAACCCTG 
GAGGTCAACC TTCTGAGTTA TGTGGTCCTG AGCACAGCCG CCTTGCCCAT GCTGAAGCAG 
ACCAACGGGA GCATTGTGGT CGTCTCCTCC ATAGCCGGGA AAATAGCTTG TCCACTTGTT 
GCTGCGTATT CTGCAAGCAA GTTTGCTCTG GATGGGTTCT TCTCCTCTCT CAGGAAGGAA 
TATGAGGCGA CCAAGGTCAA TGTGTCAATC ACCCTCTGTA TTCTTGGCCT CATCGACACA 
GACACAGCCA TGAAGGCAGT TGCTGGGATA TTTAATGCAA AAGCATCTCC AAAGGAAGAA 
TGTGCCCTGG AGATTATCAA AGGGGGAACT CTGCGCCAAG ATGAAGTATA TTATGACAGT 
TCAATCCTGA CTCCTCTCCT GCTGAGAAAT CCAGGAAGGA AGATCATGGA ATTTTTCTTC 
TTGAAGAAAT ATAATATGGA AAGATTTATA AACAACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010835165.1
CDS	126..1004
Translation	MAFMKKYLLPILGIFLAYYYYSANEEFRPEMLRGKRVIVTGASKGIGREMAYHLARMGAHVVVTARSEESLKKVVSRCLELGAASAHYVAGTMENMTFAEQFVAKAGELVGGLDMLILNHIHYTPLGVFSNDIHLLRRTLEVNLLSYVVLSTAALPMLKQTNGSIVVVSSIAGKIACPLVAAYSASKFALDGFFSSLRKEYEATKVNVSITLCILGLIDTDTAMKAVAGIFNAKASPKEECALEIIKGGTLRQDEVYYDSSILTPLLLRNPGRKIMEFFFLKKYNMERFINN

Target ORF information:

RefSeq Version	XM_010836863.1
Organism	Bison bison bison
Definition	Bison bison bison hydroxysteroid (11-beta) dehydrogenase 1 (HSD11B1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010836863.1

ORF Insert Sequence:

ATGGCTTTTA TGAAAAAATA CCTCCTCCCC ATCCTGGGGA TCTTCTTGGC CTACTACTAC 
TATTCTGCGA ATGAGGAATT CAGACCAGAG ATGCTTCGAG GAAAGAGAGT GATTGTCACG 
GGGGCCAGCA AGGGAATCGG AAGAGAAATG GCCTATCACC TGGCAAGGAT GGGAGCACAT 
GTGGTAGTGA CAGCGAGGTC AGAAGAAAGT CTAAAGAAGG TGGTATCCCG CTGCCTGGAG 
CTGGGGGCGG CCTCAGCACA CTACGTGGCG GGCACCATGG AGAACATGAC CTTCGCAGAA 
CAATTTGTTG CCAAAGCTGG AGAGCTCGTG GGGGGACTTG ACATGCTCAT TCTCAACCAC 
ATCCACTACA CGCCTTTGGG GGTGTTTAGC AATGACATCC ATCTCTTGCG CAGAACCCTG 
GAGGTCAACC TTCTGAGTTA TGTGGTCCTG AGCACAGCCG CCTTGCCCAT GCTGAAGCAG 
ACCAACGGGA GCATTGTGGT CGTCTCCTCC ATAGCCGGGA AAATAGCTTG TCCACTTGTT 
GCTGCGTATT CTGCAAGCAA GTTTGCTCTG GATGGGTTCT TCTCCTCTCT CAGGAAGGAA 
TATGAGGCGA CCAAGGTCAA TGTGTCAATC ACCCTCTGTA TTCTTGGCCT CATCGACACA 
GACACAGCCA TGAAGGCAGT TGCTGGGATA TTTAATGCAA AAGCATCTCC AAAGGAAGAA 
TGTGCCCTGG AGATTATCAA AGGGGGAACT CTGCGCCAAG ATGAAGTATA TTATGACAGT 
TCAATCCTGA CTCCTCTCCT GCTGAGAAAT CCAGGAAGGA AGATCATGGA ATTTTTCTTC 
TTGAAGAAAT ATAATATGGA AAGATTTATA AACAACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.