DBT cDNA ORF clone, Aquila chrysaetos canadensis

Gene
Clones
gRNAs

Summary

Gene Symbol	DBT
Entrez Gene ID	105412897
Full Name	dihydrolipoamide branched chain transacylase E2
Gene Type	protein-coding
Organism	Aquila chrysaetos canadensis

mRNA and Protein(s)

mRNA	Protein	Name
XM_011597014.1	XP_011595316.1	lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex, mitochondrial isoform X2
XM_011597013.1	XP_011595315.1	lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex, mitochondrial isoform X1

Pathways from BioSystems
Gene Ontology

The following DBT gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DBT cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAq30926	XM_011597014.1 Latest version!	Aquila chrysaetos canadensis dihydrolipoamide branched chain transacylase E2 (DBT), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00
OAq30925	XM_011597013.1 Latest version!	Aquila chrysaetos canadensis dihydrolipoamide branched chain transacylase E2 (DBT), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DBT ( XM_011597014.1 ) cDNA ORF clone, Aquila chrysaetos canadensis -> XP_011595316.1 Aquila chrysaetos canadensis dihydrolipoamide branched chain transacylase E2 (DBT), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OAq30926
Clone ID Related Accession (Same CDS sequence)	XM_011597014.1
Accession Version	XM_011597014.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1482bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-12
Organism	Aquila chrysaetos canadensis
Product	lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex, mitochondrial isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011951018.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Aquila chrysaetos canadensis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCCG TGACCGCTGC GAGGAGCAGC TGCCGAGCCG CGGGGCGCCT GGTTTGCATT 
CACCGTGTTA GATCGTGCAG CAGCATTCGC TTTATAAAAT CAAAATATGT GTGCGTGTTT 
GACAAATCTG CCCTCAAGTT TAGTCATCAA CAGCGATTAT TCAGAACATC TGCTGTTTCA 
TGTGGCCAAA TTGTCCAGTT TAAGCTTTCT GACATTGGAG AAGGGATTAC AGAGGTGACT 
GTAAAAGAAT GGTATATAAA AGAAGGTGAT AGCGTGTCTC AATTTGATAG CATCTGTGAA 
GTACAAAGTG ATAAAGCTTC CGTTACTATT ACTAGTCGTT ATGACGGCAT CATTAGAAAA 
CTCCATTACA ATTTGGATGA AATTGCTTAT GTTGGAAAAC CGTTAGTGGA CATTGAAATT 
GATGCCTCAA AAGGTGTTGC CCCAGAAGAA GATGTTGTTG AAACACCTCC TATGTCTCAT 
GAAGAGCACA CTCACCAAGA GATAAAAGGT CACAAAACAT TAGCAACCCC TGCAGTTCGT 
CGTCTGGCCA TGGAGAACAA TATTAAATTG AGTGAAGTTG TTGGAACAGG GAAAGATAAC 
AGAATCCTTA AAGAAGACAT ACTCAATTAC TTAGCCAAAC AAACAGGAGC TATTTTACCT 
CCATCACCGA AGGCTGAAAT TATCCCACCT TTGCCAAAAT CAGAGACTCT GCCAGCTGCT 
CCAAAGGACA AAGCACGCAA AATTCCTGTA CCTGTTTCCA GACCCATTGT GTTTTCAGGA 
AAAGATAAAA CTGAACCTGT AACAGGTTTT CACAAGGCAA TGGTGAAGAC TATGAGTGCA 
GCCTTGAAGA TACCCCACTT CGGTTATTGT GATGAGATTG ATTTGACTCA GCTTGTTCAG 
TTGCGAGAAG AGCTGAAACC TCTAGCACAA ATTCGTGGAG TTAAGCTTTC CTTTATGCCT 
TTCTTTATAA AGGCAGCCTC TCTGGGATTA TTGCAGTATC CCATTCTTAA TGCTTCTTTG 
GATGAAAGCT GTCAAAACAT CACATATAAG GCTTCGCACA ACATTGGAGT TGCTATGGAC 
ACAGAGCAAG GTTTAATTGT TCCAAATGTG AAAAATGTTC AGGTCTTGAG TGTGTTTGAG 
ATTGCTTCCG AATTAAATCG CCTACAGTCC TTGGGCTCTG CAGGCCAGCT GGGAACAAAT 
GACCTCACTG GGGGAACATT CACCCTTTCA AATATTGGCA CAATTGGTGG CACTTACACC 
AAACCAGTGA TACTACCTCC TGAAGTAGCT ATTGGAGCAC TTGGAAAGAT ACAGGTTCTT 
CCTCGGTTTA ATGGAAAAGG TGAAGTATTT AAAGCACAGA TAATGAATGT GAGTTGGTCA 
GCTGATCACC GCATCATTGA TGGAGCTACA ATGGCCCGGT TTTCTAACTT GTGGAAATCT 
TACTTGGAGA ACCCTGCTTC GATGCTGCTA GACCTTAAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011595316.1
CDS	54..1535
Translation	MAAVTAARSSCRAAGRLVCIHRVRSCSSIRFIKSKYVCVFDKSALKFSHQQRLFRTSAVSCGQIVQFKLSDIGEGITEVTVKEWYIKEGDSVSQFDSICEVQSDKASVTITSRYDGIIRKLHYNLDEIAYVGKPLVDIEIDASKGVAPEEDVVETPPMSHEEHTHQEIKGHKTLATPAVRRLAMENNIKLSEVVGTGKDNRILKEDILNYLAKQTGAILPPSPKAEIIPPLPKSETLPAAPKDKARKIPVPVSRPIVFSGKDKTEPVTGFHKAMVKTMSAALKIPHFGYCDEIDLTQLVQLREELKPLAQIRGVKLSFMPFFIKAASLGLLQYPILNASLDESCQNITYKASHNIGVAMDTEQGLIVPNVKNVQVLSVFEIASELNRLQSLGSAGQLGTNDLTGGTFTLSNIGTIGGTYTKPVILPPEVAIGALGKIQVLPRFNGKGEVFKAQIMNVSWSADHRIIDGATMARFSNLWKSYLENPASMLLDLK

Target ORF information:

RefSeq Version	XM_011597014.1
Organism	Aquila chrysaetos canadensis
Definition	Aquila chrysaetos canadensis dihydrolipoamide branched chain transacylase E2 (DBT), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011597014.1

ORF Insert Sequence:

ATGGCGGCCG TGACCGCTGC GAGGAGCAGC TGCCGAGCCG CGGGGCGCCT GGTTTGCATT 
CACCGTGTTA GATCGTGCAG CAGCATTCGC TTTATAAAAT CAAAATATGT GTGCGTGTTT 
GACAAATCTG CCCTCAAGTT TAGTCATCAA CAGCGATTAT TCAGAACATC TGCTGTTTCA 
TGTGGCCAAA TTGTCCAGTT TAAGCTTTCT GACATTGGAG AAGGGATTAC AGAGGTGACT 
GTAAAAGAAT GGTATATAAA AGAAGGTGAT AGCGTGTCTC AATTTGATAG CATCTGTGAA 
GTACAAAGTG ATAAAGCTTC CGTTACTATT ACTAGTCGTT ATGACGGCAT CATTAGAAAA 
CTCCATTACA ATTTGGATGA AATTGCTTAT GTTGGAAAAC CGTTAGTGGA CATTGAAATT 
GATGCCTCAA AAGGTGTTGC CCCAGAAGAA GATGTTGTTG AAACACCTCC TATGTCTCAT 
GAAGAGCACA CTCACCAAGA GATAAAAGGT CACAAAACAT TAGCAACCCC TGCAGTTCGT 
CGTCTGGCCA TGGAGAACAA TATTAAATTG AGTGAAGTTG TTGGAACAGG GAAAGATAAC 
AGAATCCTTA AAGAAGACAT ACTCAATTAC TTAGCCAAAC AAACAGGAGC TATTTTACCT 
CCATCACCGA AGGCTGAAAT TATCCCACCT TTGCCAAAAT CAGAGACTCT GCCAGCTGCT 
CCAAAGGACA AAGCACGCAA AATTCCTGTA CCTGTTTCCA GACCCATTGT GTTTTCAGGA 
AAAGATAAAA CTGAACCTGT AACAGGTTTT CACAAGGCAA TGGTGAAGAC TATGAGTGCA 
GCCTTGAAGA TACCCCACTT CGGTTATTGT GATGAGATTG ATTTGACTCA GCTTGTTCAG 
TTGCGAGAAG AGCTGAAACC TCTAGCACAA ATTCGTGGAG TTAAGCTTTC CTTTATGCCT 
TTCTTTATAA AGGCAGCCTC TCTGGGATTA TTGCAGTATC CCATTCTTAA TGCTTCTTTG 
GATGAAAGCT GTCAAAACAT CACATATAAG GCTTCGCACA ACATTGGAGT TGCTATGGAC 
ACAGAGCAAG GTTTAATTGT TCCAAATGTG AAAAATGTTC AGGTCTTGAG TGTGTTTGAG 
ATTGCTTCCG AATTAAATCG CCTACAGTCC TTGGGCTCTG CAGGCCAGCT GGGAACAAAT 
GACCTCACTG GGGGAACATT CACCCTTTCA AATATTGGCA CAATTGGTGG CACTTACACC 
AAACCAGTGA TACTACCTCC TGAAGTAGCT ATTGGAGCAC TTGGAAAGAT ACAGGTTCTT 
CCTCGGTTTA ATGGAAAAGG TGAAGTATTT AAAGCACAGA TAATGAATGT GAGTTGGTCA 
GCTGATCACC GCATCATTGA TGGAGCTACA ATGGCCCGGT TTTCTAACTT GTGGAAATCT 
TACTTGGAGA ACCCTGCTTC GATGCTGCTA GACCTTAAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DBT ( XM_011597013.1 ) cDNA ORF clone, Aquila chrysaetos canadensis -> XP_011595315.1 Aquila chrysaetos canadensis dihydrolipoamide branched chain transacylase E2 (DBT), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OAq30925
Clone ID Related Accession (Same CDS sequence)	XM_011597013.1
Accession Version	XM_011597013.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1584bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-12
Organism	Aquila chrysaetos canadensis
Product	lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011951018.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Aquila chrysaetos canadensis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTAAAAA CACATGGAAA GCGTTATGAA ACTTCACATT TAGAAATAGT TCAGAGATTT 
GATGTGCTCC TTGTGATCAT CTTCGCAGGT GTGCTGTTTT CTGGCTCCTC TAAGAAGATA 
ATTTCTAAAT TGTACAGTTT TGAGCTACCT GAGGTTTGCA TTCACCGTGT TAGATCGTGC 
AGCAGCATTC GCTTTATAAA ATCAAAATAT GTGTGCGTGT TTGACAAATC TGCCCTCAAG 
TTTAGTCATC AACAGCGATT ATTCAGAACA TCTGCTGTTT CATGTGGCCA AATTGTCCAG 
TTTAAGCTTT CTGACATTGG AGAAGGGATT ACAGAGGTGA CTGTAAAAGA ATGGTATATA 
AAAGAAGGTG ATAGCGTGTC TCAATTTGAT AGCATCTGTG AAGTACAAAG TGATAAAGCT 
TCCGTTACTA TTACTAGTCG TTATGACGGC ATCATTAGAA AACTCCATTA CAATTTGGAT 
GAAATTGCTT ATGTTGGAAA ACCGTTAGTG GACATTGAAA TTGATGCCTC AAAAGGTGTT 
GCCCCAGAAG AAGATGTTGT TGAAACACCT CCTATGTCTC ATGAAGAGCA CACTCACCAA 
GAGATAAAAG GTCACAAAAC ATTAGCAACC CCTGCAGTTC GTCGTCTGGC CATGGAGAAC 
AATATTAAAT TGAGTGAAGT TGTTGGAACA GGGAAAGATA ACAGAATCCT TAAAGAAGAC 
ATACTCAATT ACTTAGCCAA ACAAACAGGA GCTATTTTAC CTCCATCACC GAAGGCTGAA 
ATTATCCCAC CTTTGCCAAA ATCAGAGACT CTGCCAGCTG CTCCAAAGGA CAAAGCACGC 
AAAATTCCTG TACCTGTTTC CAGACCCATT GTGTTTTCAG GAAAAGATAA AACTGAACCT 
GTAACAGGTT TTCACAAGGC AATGGTGAAG ACTATGAGTG CAGCCTTGAA GATACCCCAC 
TTCGGTTATT GTGATGAGAT TGATTTGACT CAGCTTGTTC AGTTGCGAGA AGAGCTGAAA 
CCTCTAGCAC AAATTCGTGG AGTTAAGCTT TCCTTTATGC CTTTCTTTAT AAAGGCAGCC 
TCTCTGGGAT TATTGCAGTA TCCCATTCTT AATGCTTCTT TGGATGAAAG CTGTCAAAAC 
ATCACATATA AGGCTTCGCA CAACATTGGA GTTGCTATGG ACACAGAGCA AGGTTTAATT 
GTTCCAAATG TGAAAAATGT TCAGGTCTTG AGTGTGTTTG AGATTGCTTC CGAATTAAAT 
CGCCTACAGT CCTTGGGCTC TGCAGGCCAG CTGGGAACAA ATGACCTCAC TGGGGGAACA 
TTCACCCTTT CAAATATTGG CACAATTGGT GGCACTTACA CCAAACCAGT GATACTACCT 
CCTGAAGTAG CTATTGGAGC ACTTGGAAAG ATACAGGTTC TTCCTCGGTT TAATGGAAAA 
GGTGAAGTAT TTAAAGCACA GATAATGAAT GTGAGTTGGT CAGCTGATCA CCGCATCATT 
GATGGAGCTA CAATGGCCCG GTTTTCTAAC TTGTGGAAAT CTTACTTGGA GAACCCTGCT 
TCGATGCTGC TAGACCTTAA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011595315.1
CDS	1546..3129
Translation	MLKTHGKRYETSHLEIVQRFDVLLVIIFAGVLFSGSSKKIISKLYSFELPEVCIHRVRSCSSIRFIKSKYVCVFDKSALKFSHQQRLFRTSAVSCGQIVQFKLSDIGEGITEVTVKEWYIKEGDSVSQFDSICEVQSDKASVTITSRYDGIIRKLHYNLDEIAYVGKPLVDIEIDASKGVAPEEDVVETPPMSHEEHTHQEIKGHKTLATPAVRRLAMENNIKLSEVVGTGKDNRILKEDILNYLAKQTGAILPPSPKAEIIPPLPKSETLPAAPKDKARKIPVPVSRPIVFSGKDKTEPVTGFHKAMVKTMSAALKIPHFGYCDEIDLTQLVQLREELKPLAQIRGVKLSFMPFFIKAASLGLLQYPILNASLDESCQNITYKASHNIGVAMDTEQGLIVPNVKNVQVLSVFEIASELNRLQSLGSAGQLGTNDLTGGTFTLSNIGTIGGTYTKPVILPPEVAIGALGKIQVLPRFNGKGEVFKAQIMNVSWSADHRIIDGATMARFSNLWKSYLENPASMLLDLK

Target ORF information:

RefSeq Version	XM_011597013.1
Organism	Aquila chrysaetos canadensis
Definition	Aquila chrysaetos canadensis dihydrolipoamide branched chain transacylase E2 (DBT), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011597013.1

ORF Insert Sequence:

ATGTTAAAAA CACATGGAAA GCGTTATGAA ACTTCACATT TAGAAATAGT TCAGAGATTT 
GATGTGCTCC TTGTGATCAT CTTCGCAGGT GTGCTGTTTT CTGGCTCCTC TAAGAAGATA 
ATTTCTAAAT TGTACAGTTT TGAGCTACCT GAGGTTTGCA TTCACCGTGT TAGATCGTGC 
AGCAGCATTC GCTTTATAAA ATCAAAATAT GTGTGCGTGT TTGACAAATC TGCCCTCAAG 
TTTAGTCATC AACAGCGATT ATTCAGAACA TCTGCTGTTT CATGTGGCCA AATTGTCCAG 
TTTAAGCTTT CTGACATTGG AGAAGGGATT ACAGAGGTGA CTGTAAAAGA ATGGTATATA 
AAAGAAGGTG ATAGCGTGTC TCAATTTGAT AGCATCTGTG AAGTACAAAG TGATAAAGCT 
TCCGTTACTA TTACTAGTCG TTATGACGGC ATCATTAGAA AACTCCATTA CAATTTGGAT 
GAAATTGCTT ATGTTGGAAA ACCGTTAGTG GACATTGAAA TTGATGCCTC AAAAGGTGTT 
GCCCCAGAAG AAGATGTTGT TGAAACACCT CCTATGTCTC ATGAAGAGCA CACTCACCAA 
GAGATAAAAG GTCACAAAAC ATTAGCAACC CCTGCAGTTC GTCGTCTGGC CATGGAGAAC 
AATATTAAAT TGAGTGAAGT TGTTGGAACA GGGAAAGATA ACAGAATCCT TAAAGAAGAC 
ATACTCAATT ACTTAGCCAA ACAAACAGGA GCTATTTTAC CTCCATCACC GAAGGCTGAA 
ATTATCCCAC CTTTGCCAAA ATCAGAGACT CTGCCAGCTG CTCCAAAGGA CAAAGCACGC 
AAAATTCCTG TACCTGTTTC CAGACCCATT GTGTTTTCAG GAAAAGATAA AACTGAACCT 
GTAACAGGTT TTCACAAGGC AATGGTGAAG ACTATGAGTG CAGCCTTGAA GATACCCCAC 
TTCGGTTATT GTGATGAGAT TGATTTGACT CAGCTTGTTC AGTTGCGAGA AGAGCTGAAA 
CCTCTAGCAC AAATTCGTGG AGTTAAGCTT TCCTTTATGC CTTTCTTTAT AAAGGCAGCC 
TCTCTGGGAT TATTGCAGTA TCCCATTCTT AATGCTTCTT TGGATGAAAG CTGTCAAAAC 
ATCACATATA AGGCTTCGCA CAACATTGGA GTTGCTATGG ACACAGAGCA AGGTTTAATT 
GTTCCAAATG TGAAAAATGT TCAGGTCTTG AGTGTGTTTG AGATTGCTTC CGAATTAAAT 
CGCCTACAGT CCTTGGGCTC TGCAGGCCAG CTGGGAACAA ATGACCTCAC TGGGGGAACA 
TTCACCCTTT CAAATATTGG CACAATTGGT GGCACTTACA CCAAACCAGT GATACTACCT 
CCTGAAGTAG CTATTGGAGC ACTTGGAAAG ATACAGGTTC TTCCTCGGTT TAATGGAAAA 
GGTGAAGTAT TTAAAGCACA GATAATGAAT GTGAGTTGGT CAGCTGATCA CCGCATCATT 
GATGGAGCTA CAATGGCCCG GTTTTCTAAC TTGTGGAAAT CTTACTTGGA GAACCCTGCT 
TCGATGCTGC TAGACCTTAA ATAA