DGKI cDNA ORF clone, Aquila chrysaetos canadensis

Gene
Clones
gRNAs

Summary

Gene Symbol	DGKI
Entrez Gene ID	105401957
Full Name	diacylglycerol kinase iota
Gene Type	protein-coding
Organism	Aquila chrysaetos canadensis

mRNA and Protein(s)

mRNA	Protein	Name
XM_011576746.1	XP_011575048.1	diacylglycerol kinase iota

Pathways from BioSystems
Gene Ontology

The following DGKI gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DGKI cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAq16060	XM_011576746.1 Latest version!	Aquila chrysaetos canadensis diacylglycerol kinase, iota (DGKI), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DGKI ( XM_011576746.1 ) cDNA ORF clone, Aquila chrysaetos canadensis -> XP_011575048.1 Aquila chrysaetos canadensis diacylglycerol kinase, iota (DGKI), mRNA.

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CloneID	OAq16060
Clone ID Related Accession (Same CDS sequence)	XM_011576746.1
Accession Version	XM_011576746.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2607bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-12
Organism	Aquila chrysaetos canadensis
Product	diacylglycerol kinase iota
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011950887.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Aquila chrysaetos canadensis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCAGT TGGAGAAGAT TAATTTTCGC TGCAAACCAA CTTTCCGAGA AGGAGGCTCC 
AGATCTCCAA GAGAAAACTT TGTCCGACAT CACTGGGTGC ACAGGCGGAG GCAGGAGGGG 
AAATGTAAGC AGTGTGGAAA GGGCTTTCAG CAGAAATTCT CCTTCCACAG TAAAGAGATT 
GTGGCCATCA GTTGTTCCTG GTGCAAGCTG GCGTTTCATA ACAAAGTCAC TTGTTTCATG 
CTACATCACA TTGAGGAGCC ATGTTCCCTG GGGGCTCACG CTGCTGTCAT TGTGCCTCCC 
ACCTGGATCA TTAAGGTGAA GAAACCTCAG GTGGTCTCCA TACCAGGAGA CGAGCCTGAT 
TGGGCAGGAG ACTGGAGCCC CGAAGGGGAA ACGGGCGTCT CCTACCCCTA TTGGGAGGGT 
TGGCAGAACT CATTAAAGAC TTCAACACGG CGAAAGAAGA GAACATCTTT TAAAAGAAAA 
GCCAGCAAAA GAGGCAATGA AGATAACAAA GGTCGGCCAT TTGTGATAAA GCCTATCTCC 
TCTCCACTCA TGAAGCCGCT GCTGGTTTTT GTCAATCCAA AAAGTGGAGG GAATCAGGGC 
ACCAAGGTTC TCCAAATGTT TATGTGGTAT TTGAATCCAC GCCAGGTCTT TGATCTCTCT 
CAGGACGGGC CAAGAGATGC GCTGGAGCTG TACAGAAAAG TGCCAAACCT GCGGATCCTG 
GCCTGCGGCG GGGACGGGAC GGTGGGCTGG ATCCTCTCCA TCCTTGATGA GCTGCAACTC 
AGCCCCCCGC CGCCTGTGGC TGTCCTTCCG CTGGGCACCG GGAATGACCT TGCCCGCACC 
CTCAACTGGG GTGGGGGTTA CACCGATGAG CCAGTGTCCA AGATCCTGTG CCATGTAGAA 
GATGGGACCA TTGTCCAGCT GGACCGCTGG AATCTCCAGG TTGAGCGCAA CCCTGATTTG 
CCACAGGATG AGCTGGAGGA TGGGGCACGT AAGCTTCCTC TCAGTGTCTT CAATAATTAC 
TTCAGCCTTG GATTTGATGC ACATGTTACA CTGGAGTTCC ATGAATCTAG AGAAGCAAAT 
CCAGAGAAAT TCAACAGCCG ATTTAGGAAT AAAATGTTTT ATGCAGGGGC AGCCTTTACA 
GACTTTCTGC AAAGAAGCTC AAGAGATTTA TCCAAGCATG TTAAAGTTGT GTGTGATGGT 
ACAGACCTGA CTCCAAAGAT CCAGGAGCTG AAGTTCCAGT GTATAGTGTT TTTAAACATA 
CCCAGGTATT GTGCTGGCAC AATGCCCTGG GGAAACCCTG GAGATCACAG AGACTTTGAG 
CCTCAGCGTC ATGATGATGG CTACATTGAA GTCATTGGGT TCACCATGGC CTCACTGGCT 
GCTCTCCAGG TGGGCGGTCA TGGAGAGAGG TTGCATCAGT GCCGTGAGGT GACACTTTTG 
ACATACAAGT CTATTCCAAT GCAAGTGGAT GGTGAACCGT GTCGATTAGC TCCCTCACTC 
ATTCGAATCT CCTTAAGGAA CCAAGCCAAC ATGGTGCAGA AGAGCAAGAG GAGAACATCC 
ATGCCTTTGC TGAATGATCC TCATTCCATC CCAGATCGCC TGCGGATCAG AGTGAACAGG 
ATTAATTTAC AAGAATATGA GGGGCTACAT TACGACAAGG AAAAACTCCG GGAAGCCTCC 
ATTCCCCTAG GAATTATAGT TGTACGAGGA GATTGTGACT TGGAGACCTG TCGTATGTAC 
ATTGACCGTC TACAAGAGGA CCTACAGTCA GTAACTTCTA CTACACAGAG AGTTCATTAC 
CAGGACCAGG AAAGCTCTTT CCCCAGAGTA CTTTCTGTTC AGAGGCTATC TCCTAGATGG 
TGCTTCCTAG ACGCAACTTC TGCTGATCGT TTTTACCGCA TTGACAGATC TCAGGAACAT 
TTGCACTTTG TGACGGAAAT TTCGCAGGAC GAGATTTTTA TTTTGGACCC AGAGCTGGTA 
ATGTCACAGC AGGTGGGGAC GCCACCAGGA ATGCCTGATC TGGTAGTGGA GCAGGCCTCT 
GGAATATCAG ATCGGTGGAA CCCTGCGGTT CGAAAGAGGA TGCTGAGCGA CAGCGGCCTT 
GGCAAGATTT CCCCACACTA CGAGGTACCT GACAAACAAA AGGACGCCAG CCAGACACAT 
ATGCTGCAGT CACCAGTCTC TTCAGAAGAT CAAGCACTTT TACAGGCAGT CATAGCTGGT 
GATCTGCTGA AGTTCATAGA ATGCTGTAAA AAAGGAGCCA ATTTGTTAAT CCAAGGACCT 
GATCACTGCT CCTTGTTACA CCATGCTGCC AAGACTGGGC ATGGCGAGAT TGTGAAATAC 
ATCCTAGAGC ATGGTCCATC TGAATTACTG GACATGACAG ACAGTGAAAC GGGTGAGACA 
GCATTACATA AGGCAGCCTG CCAGCGCCAC CGCGCCGTCT GCCAGCTCCT GGTGGATGCG 
GGAGCCTCTC TGAGAAAGAC GGACTCCAAG GGTAAGACCC CTCGTGACAG GGCTCAGCAA 
GCTGGTGACC CAGATCTGGC CTCCTACCTG GAGAGTCGAC AAAACTACCA AATGGTTTCA 
CATGAGGATC TGGAGACAGC AGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011575048.1
CDS	257..2863
Translation	MEQLEKINFRCKPTFREGGSRSPRENFVRHHWVHRRRQEGKCKQCGKGFQQKFSFHSKEIVAISCSWCKLAFHNKVTCFMLHHIEEPCSLGAHAAVIVPPTWIIKVKKPQVVSIPGDEPDWAGDWSPEGETGVSYPYWEGWQNSLKTSTRRKKRTSFKRKASKRGNEDNKGRPFVIKPISSPLMKPLLVFVNPKSGGNQGTKVLQMFMWYLNPRQVFDLSQDGPRDALELYRKVPNLRILACGGDGTVGWILSILDELQLSPPPPVAVLPLGTGNDLARTLNWGGGYTDEPVSKILCHVEDGTIVQLDRWNLQVERNPDLPQDELEDGARKLPLSVFNNYFSLGFDAHVTLEFHESREANPEKFNSRFRNKMFYAGAAFTDFLQRSSRDLSKHVKVVCDGTDLTPKIQELKFQCIVFLNIPRYCAGTMPWGNPGDHRDFEPQRHDDGYIEVIGFTMASLAALQVGGHGERLHQCREVTLLTYKSIPMQVDGEPCRLAPSLIRISLRNQANMVQKSKRRTSMPLLNDPHSIPDRLRIRVNRINLQEYEGLHYDKEKLREASIPLGIIVVRGDCDLETCRMYIDRLQEDLQSVTSTTQRVHYQDQESSFPRVLSVQRLSPRWCFLDATSADRFYRIDRSQEHLHFVTEISQDEIFILDPELVMSQQVGTPPGMPDLVVEQASGISDRWNPAVRKRMLSDSGLGKISPHYEVPDKQKDASQTHMLQSPVSSEDQALLQAVIAGDLLKFIECCKKGANLLIQGPDHCSLLHHAAKTGHGEIVKYILEHGPSELLDMTDSETGETALHKAACQRHRAVCQLLVDAGASLRKTDSKGKTPRDRAQQAGDPDLASYLESRQNYQMVSHEDLETAV

Target ORF information:

RefSeq Version	XM_011576746.1
Organism	Aquila chrysaetos canadensis
Definition	Aquila chrysaetos canadensis diacylglycerol kinase, iota (DGKI), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011576746.1

ORF Insert Sequence:

ATGGAGCAGT TGGAGAAGAT TAATTTTCGC TGCAAACCAA CTTTCCGAGA AGGAGGCTCC 
AGATCTCCAA GAGAAAACTT TGTCCGACAT CACTGGGTGC ACAGGCGGAG GCAGGAGGGG 
AAATGTAAGC AGTGTGGAAA GGGCTTTCAG CAGAAATTCT CCTTCCACAG TAAAGAGATT 
GTGGCCATCA GTTGTTCCTG GTGCAAGCTG GCGTTTCATA ACAAAGTCAC TTGTTTCATG 
CTACATCACA TTGAGGAGCC ATGTTCCCTG GGGGCTCACG CTGCTGTCAT TGTGCCTCCC 
ACCTGGATCA TTAAGGTGAA GAAACCTCAG GTGGTCTCCA TACCAGGAGA CGAGCCTGAT 
TGGGCAGGAG ACTGGAGCCC CGAAGGGGAA ACGGGCGTCT CCTACCCCTA TTGGGAGGGT 
TGGCAGAACT CATTAAAGAC TTCAACACGG CGAAAGAAGA GAACATCTTT TAAAAGAAAA 
GCCAGCAAAA GAGGCAATGA AGATAACAAA GGTCGGCCAT TTGTGATAAA GCCTATCTCC 
TCTCCACTCA TGAAGCCGCT GCTGGTTTTT GTCAATCCAA AAAGTGGAGG GAATCAGGGC 
ACCAAGGTTC TCCAAATGTT TATGTGGTAT TTGAATCCAC GCCAGGTCTT TGATCTCTCT 
CAGGACGGGC CAAGAGATGC GCTGGAGCTG TACAGAAAAG TGCCAAACCT GCGGATCCTG 
GCCTGCGGCG GGGACGGGAC GGTGGGCTGG ATCCTCTCCA TCCTTGATGA GCTGCAACTC 
AGCCCCCCGC CGCCTGTGGC TGTCCTTCCG CTGGGCACCG GGAATGACCT TGCCCGCACC 
CTCAACTGGG GTGGGGGTTA CACCGATGAG CCAGTGTCCA AGATCCTGTG CCATGTAGAA 
GATGGGACCA TTGTCCAGCT GGACCGCTGG AATCTCCAGG TTGAGCGCAA CCCTGATTTG 
CCACAGGATG AGCTGGAGGA TGGGGCACGT AAGCTTCCTC TCAGTGTCTT CAATAATTAC 
TTCAGCCTTG GATTTGATGC ACATGTTACA CTGGAGTTCC ATGAATCTAG AGAAGCAAAT 
CCAGAGAAAT TCAACAGCCG ATTTAGGAAT AAAATGTTTT ATGCAGGGGC AGCCTTTACA 
GACTTTCTGC AAAGAAGCTC AAGAGATTTA TCCAAGCATG TTAAAGTTGT GTGTGATGGT 
ACAGACCTGA CTCCAAAGAT CCAGGAGCTG AAGTTCCAGT GTATAGTGTT TTTAAACATA 
CCCAGGTATT GTGCTGGCAC AATGCCCTGG GGAAACCCTG GAGATCACAG AGACTTTGAG 
CCTCAGCGTC ATGATGATGG CTACATTGAA GTCATTGGGT TCACCATGGC CTCACTGGCT 
GCTCTCCAGG TGGGCGGTCA TGGAGAGAGG TTGCATCAGT GCCGTGAGGT GACACTTTTG 
ACATACAAGT CTATTCCAAT GCAAGTGGAT GGTGAACCGT GTCGATTAGC TCCCTCACTC 
ATTCGAATCT CCTTAAGGAA CCAAGCCAAC ATGGTGCAGA AGAGCAAGAG GAGAACATCC 
ATGCCTTTGC TGAATGATCC TCATTCCATC CCAGATCGCC TGCGGATCAG AGTGAACAGG 
ATTAATTTAC AAGAATATGA GGGGCTACAT TACGACAAGG AAAAACTCCG GGAAGCCTCC 
ATTCCCCTAG GAATTATAGT TGTACGAGGA GATTGTGACT TGGAGACCTG TCGTATGTAC 
ATTGACCGTC TACAAGAGGA CCTACAGTCA GTAACTTCTA CTACACAGAG AGTTCATTAC 
CAGGACCAGG AAAGCTCTTT CCCCAGAGTA CTTTCTGTTC AGAGGCTATC TCCTAGATGG 
TGCTTCCTAG ACGCAACTTC TGCTGATCGT TTTTACCGCA TTGACAGATC TCAGGAACAT 
TTGCACTTTG TGACGGAAAT TTCGCAGGAC GAGATTTTTA TTTTGGACCC AGAGCTGGTA 
ATGTCACAGC AGGTGGGGAC GCCACCAGGA ATGCCTGATC TGGTAGTGGA GCAGGCCTCT 
GGAATATCAG ATCGGTGGAA CCCTGCGGTT CGAAAGAGGA TGCTGAGCGA CAGCGGCCTT 
GGCAAGATTT CCCCACACTA CGAGGTACCT GACAAACAAA AGGACGCCAG CCAGACACAT 
ATGCTGCAGT CACCAGTCTC TTCAGAAGAT CAAGCACTTT TACAGGCAGT CATAGCTGGT 
GATCTGCTGA AGTTCATAGA ATGCTGTAAA AAAGGAGCCA ATTTGTTAAT CCAAGGACCT 
GATCACTGCT CCTTGTTACA CCATGCTGCC AAGACTGGGC ATGGCGAGAT TGTGAAATAC 
ATCCTAGAGC ATGGTCCATC TGAATTACTG GACATGACAG ACAGTGAAAC GGGTGAGACA 
GCATTACATA AGGCAGCCTG CCAGCGCCAC CGCGCCGTCT GCCAGCTCCT GGTGGATGCG 
GGAGCCTCTC TGAGAAAGAC GGACTCCAAG GGTAAGACCC CTCGTGACAG GGCTCAGCAA 
GCTGGTGACC CAGATCTGGC CTCCTACCTG GAGAGTCGAC AAAACTACCA AATGGTTTCA 
CATGAGGATC TGGAGACAGC AGTCTGA