RAPGEF2 cDNA ORF clone, Aquila chrysaetos canadensis

Gene
Clones
gRNAs

Summary

Gene Symbol	RAPGEF2
Entrez Gene ID	105400527
Full Name	Rap guanine nucleotide exchange factor 2
Gene Type	protein-coding
Organism	Aquila chrysaetos canadensis

mRNA and Protein(s)

mRNA	Protein	Name
XM_011574274.1	XP_011572576.1	rap guanine nucleotide exchange factor 2 isoform X5
XM_011574270.1	XP_011572572.1	rap guanine nucleotide exchange factor 2 isoform X1
XM_011574273.1	XP_011572575.1	rap guanine nucleotide exchange factor 2 isoform X4
XM_011574276.1	XP_011572578.1	rap guanine nucleotide exchange factor 2 isoform X6
XM_011574271.1	XP_011572573.1	rap guanine nucleotide exchange factor 2 isoform X2
XM_011574272.1	XP_011572574.1	rap guanine nucleotide exchange factor 2 isoform X3

Pathways from BioSystems
Gene Ontology

The following RAPGEF2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RAPGEF2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OAq14197	XM_011574273.1 Latest version!	Aquila chrysaetos canadensis Rap guanine nucleotide exchange factor (GEF) 2 (RAPGEF2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OAq14198	XM_011574274.1 Latest version!	Aquila chrysaetos canadensis Rap guanine nucleotide exchange factor (GEF) 2 (RAPGEF2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OAq14199	XM_011574276.1 Latest version!	Aquila chrysaetos canadensis Rap guanine nucleotide exchange factor (GEF) 2 (RAPGEF2), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OAq14194	XM_011574270.1 Latest version!	Aquila chrysaetos canadensis Rap guanine nucleotide exchange factor (GEF) 2 (RAPGEF2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OAq14195	XM_011574271.1 Latest version!	Aquila chrysaetos canadensis Rap guanine nucleotide exchange factor (GEF) 2 (RAPGEF2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OAq14196	XM_011574272.1 Latest version!	Aquila chrysaetos canadensis Rap guanine nucleotide exchange factor (GEF) 2 (RAPGEF2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RAPGEF2 ( XM_011574273.1 ) cDNA ORF clone, Aquila chrysaetos canadensis -> XP_011572575.1 Aquila chrysaetos canadensis Rap guanine nucleotide exchange factor (GEF) 2 (RAPGEF2), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OAq14197
Clone ID Related Accession (Same CDS sequence)	XM_011574273.1
Accession Version	XM_011574273.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4476bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-12
Organism	Aquila chrysaetos canadensis
Product	rap guanine nucleotide exchange factor 2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011950881.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Aquila chrysaetos canadensis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAACCAC TTGCAATCCC AGCTAACCAT GGAGTTATGG GCCAGCAGGA GAAACACTCA 
CTTCCTGCAG ATTTCACAAA GCTGCACCTT ACTGACAGTC TCCACCCACA GGTGACCCAC 
GTTTCATCTA GTCACTCAGG ATGTAGCATC ACTAGTGATT CAGGAAGCAG CAGCCTTTCT 
GATATCTACC AGGCTACAGA AAGTGAGGCT GGTGATATGG ATCTTAGTGG GTTGCCAGAG 
ACAGCAGTGG ATTCTGAGGA TGATGATGAT GAAGAAGACA TTGAAAGGGC ATCGGATCCT 
TTGATGAGTA GGGATATTGT TAGAGACTGC TTGGAGAAAG ACCCAATAGA CAGAACAGAT 
GATGACATTG AACAACTACT GGAATTCATG CATCAATTGC CTGCTTTCGC CAACATGACA 
ATGTCAGTGA GGAGGGAGCT CTGTGCTGTG ATGGTGTTTG CAGTTGTGGA GAGAGCAGGA 
ACTATTGTAC TGAACGATGG GGAAGAGCTG GATTCCTGGT CAGTCATTTT GAATGGCTCT 
GTGGAGGTGA CGTACCCTGA TGGAAGAACA GAGATACTCT GCATGGGGAA CAGTTTTGGT 
GTTTCCCCTA CCATGGAAAA GGAATATATG AAAGGAGTGA TGAGAACCAA AGTGGATGAC 
TGCCAGTTTG TTTGTATAGC CCAGCAAGAT TATTGCCGCA TCCTCAATCA AGTAGAAAAG 
AACATGCAGA AGGTAGAAGA GGAAGGGGAG ATTGTGATGG TGAAGGAGCA CAGGGAGCTT 
GATCGCACTG GAACAAGAAA AGGTCACATT GTCATCAAGG GAACAGCTGA AAGGTTAACA 
ATGCATTTGG TGGAAGAGCA CTCTGTGGTA GACCCAACAT TTATAGAGGA CTTCCTGTTG 
ACATATCGGA CCTTTCTTTC CAGCCCGATG GAAGTGGGCA AGAAGTTGTT GGAGTGGTTC 
AATGACCCCA GCCTCAGGGA TAAGGTTACA CGGGTAGTAT TGTTGTGGGT GAACAATCAC 
TTCAATGATT TTGAAGGAGA TCCTGCTATG ACTCGATTTT TGGAAGAATT TGAGAACAAT 
TTGGAGAGGG AGAAAATGGG TGGACATTTG AGGCTGTTAA ATATTGCTTG TGCTGCTAAA 
GCTAAACGAA GATTGATAAC CTTAACAAAG CCATCTCGAG AAGCCCCTTT GCCTTTTATC 
TTGCTGGGAG GGTCAGAAAA GGGATTTGGA ATCTTTGTTG ACAGTGTAGA TTTTGGTAGC 
AAAGCCACAG AAGCAGGCTT GAAACGTGGA GACCAGATAC TGGAAGTGAA TGGTCAAAAC 
TTTGAAAACA TTCAGCTGTC AAAAGCCATG GAAATCCTTA GAAATAACAC TCATCTGTCT 
ATCACTGTGA AAACAAATTT ATTTGTTTTT AAAGAACTCT TAACACGGTT GTCAGAGGAA 
AAAAGAAATG GTGCTCCCCA CCTGCCTAAA ATTGGTGATA TTAAAAAGGC GAGTCGTTAT 
TCCATCCCAG ACCTTGCTGT TGATGTGGAG CAGGTAATAG GATTAGAAAA AGTAAATAAG 
AAAAGTAAAG CCAACACAGT TGGGGGAAGA AATAAATTAA AGAAGATACT TGACAAGACT 
CGAATCAGTA TTCTGCCTCA GAAACCGTAC AATGACATTG GAATTGGCCA GTCTCAGGAT 
GACAGCATTG TGGGACTGAG GCAAACAAAG CACATTCCTC CTGCTTTACC TGTCAGCGGA 
ACCCTGTCAT CCAGTAATCC CGATCTACTG CAGTCTCATC ACCGCATCTT AGACTTCAAT 
ACTACTCCGG ACTTACCAGA TCAAGTTCTG AGGGTTTTCA AGGCAGATCA GCAAAGTCGC 
TACATCATGA TCAGTAAGGA CACAACAGCA AAAGAAGTGG TCATCCAGGC TATCAGGGAG 
TTTGCTCTCA CTGCAACCCC TGATGCATAT TCACTATGCG AAGTGTCTGT CACACCTGAG 
GGTGTGATCA AGCAAAGGAG GCTTCCTGAT CAGTTATCCA AGCTTGCTGA CAGGATACAG 
CTGAGTGGCA GGTATTACCT GAAGAACAAC ATGGAAACAG AAACTCTTTG TTCAGATGAA 
GATGCTCAAG AGTTACTAAG GGAAAGTCAA ATTTCCCTAT TACAGCTCAG TACTGTTGAG 
GTGGCTACCC AACTCTCCAT GAGAAACTTC GAGCTATTCC GTAATATTGA ACCCACGGAA 
TACATAGATG ACTTGTTTAA ACTCAAATCA AAAACAGGTT GCACTAATCT AAAAAAATTT 
GAAGAGGTGA TAAATCAAGA AACATTCTGG GTGGCTTCTG AGATTCTAAG AGAAACCAAC 
CAGCTGAAAA GGATGAAGAT CATTAAGCAT TTCATTAAGA TAGCACTACA CTGCAGAGAA 
TGCAAGAACT TCAACTCAAT GTTTGCCATC ATTAGTGGCC TGAATTTGGC ACCAGTTGCA 
AGGCTCCGAA CTACTTGGGA AAAGCTTCCA AGCAAGTATG AAAAACTGTT TCAAGATCTA 
CAGGACTTGT TTGATCCATC TAGGAATATG GCAAAATATC GTAATGTCCT TAACAGCCAA 
AACCTACAGC CCCCCATTAT ACCCCTGTTT CCTGTTATCA AAAAAGACCT TACATTCCTT 
CATGAAGGAA ATGATTCAAA AGTGGAGGGC TTGGTCAATT TTGAGAAGCT GAGAATGATT 
GCAAAGGAGA TACGCCATGT CGGTCGCATG GCATCTGTGA ACATGGATCC TGCTTTAATG 
TTTAGAACAA GGAAGAAGAA ATGGAGGAGT TTGGGGTCTC TCAGCCAGGG CAGTACAAAT 
GCAGCTGTGC TGGATGTCGC GCAAACGGGG GGTCATAAAA AGCGGGTCCG TCGCAGCTCC 
TTCCTTAATG CTAAAAAACT GTATGAAGAT GCTCAGATGG CACGGAAAGT GAAGCAGTAT 
CTCTCAAACT TAGATCTGGA AATGGATGAA GAGAGCCTCC AGACACTGTC TCTGCAGTGT 
GAGCCAGCCA CCAACACACT GCCGAAGAAT ACAGGAGACA AGCGATCTGG AAAGTCTGAA 
ACGTCACCGG TGGCTCCCCG GGCAGGCATC CAGCAGAAAG TGCAGCAGCA GCAGCAACAT 
AAAGTAAACC AAGCATTGCA GGTCCCTGCC GTATCTCTTT ATCCCTCTCG CAAGAAAGTG 
CCGGTCAAAG ACCTCCCACC ATTTGGCATT AACTCCCCAC AAGCTTTAAA GAAAATTCTT 
TCCTTATCTG AAGAAGGAAG TTTGGATCGT CACAAGAAAC AATCTGAAGA CACGGTTTCA 
AGTGCGTCTT CGCAGCTGTC TTCTCCTCCC ACTTCACCAC AGAGCTCTCC AAGGAAAGGC 
TATACTTTGG CTCCTAGCGG CACAGTGGAT AACTTTTCAG ATTCTGGTCA CAGCGAAATT 
TCTTCCAGAT CTAGTATTGT CAGCAATTCC TCTTTTGACT CTATGCCAGT CTCCCTGCAT 
GATGAGAGGA GACAGAGACA TTCTGTCAGC ATCGTGGAGA CAAATCTTGG TGTGGGAAGG 
ATTGATAGAA GAATCATGAT TGAACCAGAT CAATACAGCT TAGGCTCATA TGCACCACTG 
TCAGAGACCA GAGGCCTGTA TGCTGGAGCA ACTGTGCTTT CTTCTCCTAG TACAGAAGAA 
CTGTCACAGG ATCAGGGGGA CAGAGCTTCG CTCGATGCAG CTGACAGTGG CCGTGGTAGC 
TGGACTTCAT GTTCGAGCGG TTCTCATGAC AACATACAAA CAATACAGCA CCAGAGAAGC 
TGGGAGACTC TGCCTTTTGG ACATGCTCAT TTCGATAGTT CAGGCGATGG GGCAGGACTG 
TGGGCCTCGG GCAGTCATAT GGACCAGCTG ATGTTCCCTG ATCATGGCAC AAAGTATGGC 
AGGCAAAGTC AAGGTAGGGA GGGCCTTGAT CAAGCACAGT CCAGAGCAAG CTGGGCATCC 
TCGACAGGAT ACTGGGGAGA GGACTCGGAA GGTGATACAG GTACCATAAA GCGGAGGGGT 
GGGAAGGATG TTTCAATTGA AGCTGAAACC AGTAGCATAA CATCTGTACC AGCAGAGGAG 
TCAAAGCCAG CTCCCATGCC CACTCATATA GCAGTATCAT CAAGTAGTAC AAAGGGGCTT 
ATTGCACGAA AAGAGGGTCG ATACCGGGAA CCACCTCCAA CTCCTCCTGG TTACGTAGGA 
ATACCTATTG CTGAGTTTGC AGAAGGTGGT TCCCATCCAA CCAGAAAGCC TCCAGATTAC 
AACATAGCAC TTCAGAGGTC TAGAATGGTG GCGCGGACAT GTGAGACTCA TGGGACATCA 
ACTCCACAGC AGCAGTCACA CGGCCATTCA ACTAGCAGGC CTGTGAACAA ACCCCAGTGG 
CATAAACCAA ATGAGTCAGA TCCACGTCTT GCTCACTATC CATCTCAAGG GTTTTCCACA 
GAGGAAGATG AAGATGAACA AGTCTCTGCT GTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011572575.1
CDS	313..4788
Translation	MKPLAIPANHGVMGQQEKHSLPADFTKLHLTDSLHPQVTHVSSSHSGCSITSDSGSSSLSDIYQATESEAGDMDLSGLPETAVDSEDDDDEEDIERASDPLMSRDIVRDCLEKDPIDRTDDDIEQLLEFMHQLPAFANMTMSVRRELCAVMVFAVVERAGTIVLNDGEELDSWSVILNGSVEVTYPDGRTEILCMGNSFGVSPTMEKEYMKGVMRTKVDDCQFVCIAQQDYCRILNQVEKNMQKVEEEGEIVMVKEHRELDRTGTRKGHIVIKGTAERLTMHLVEEHSVVDPTFIEDFLLTYRTFLSSPMEVGKKLLEWFNDPSLRDKVTRVVLLWVNNHFNDFEGDPAMTRFLEEFENNLEREKMGGHLRLLNIACAAKAKRRLITLTKPSREAPLPFILLGGSEKGFGIFVDSVDFGSKATEAGLKRGDQILEVNGQNFENIQLSKAMEILRNNTHLSITVKTNLFVFKELLTRLSEEKRNGAPHLPKIGDIKKASRYSIPDLAVDVEQVIGLEKVNKKSKANTVGGRNKLKKILDKTRISILPQKPYNDIGIGQSQDDSIVGLRQTKHIPPALPVSGTLSSSNPDLLQSHHRILDFNTTPDLPDQVLRVFKADQQSRYIMISKDTTAKEVVIQAIREFALTATPDAYSLCEVSVTPEGVIKQRRLPDQLSKLADRIQLSGRYYLKNNMETETLCSDEDAQELLRESQISLLQLSTVEVATQLSMRNFELFRNIEPTEYIDDLFKLKSKTGCTNLKKFEEVINQETFWVASEILRETNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLAPVARLRTTWEKLPSKYEKLFQDLQDLFDPSRNMAKYRNVLNSQNLQPPIIPLFPVIKKDLTFLHEGNDSKVEGLVNFEKLRMIAKEIRHVGRMASVNMDPALMFRTRKKKWRSLGSLSQGSTNAAVLDVAQTGGHKKRVRRSSFLNAKKLYEDAQMARKVKQYLSNLDLEMDEESLQTLSLQCEPATNTLPKNTGDKRSGKSETSPVAPRAGIQQKVQQQQQHKVNQALQVPAVSLYPSRKKVPVKDLPPFGINSPQALKKILSLSEEGSLDRHKKQSEDTVSSASSQLSSPPTSPQSSPRKGYTLAPSGTVDNFSDSGHSEISSRSSIVSNSSFDSMPVSLHDERRQRHSVSIVETNLGVGRIDRRIMIEPDQYSLGSYAPLSETRGLYAGATVLSSPSTEELSQDQGDRASLDAADSGRGSWTSCSSGSHDNIQTIQHQRSWETLPFGHAHFDSSGDGAGLWASGSHMDQLMFPDHGTKYGRQSQGREGLDQAQSRASWASSTGYWGEDSEGDTGTIKRRGGKDVSIEAETSSITSVPAEESKPAPMPTHIAVSSSSTKGLIARKEGRYREPPPTPPGYVGIPIAEFAEGGSHPTRKPPDYNIALQRSRMVARTCETHGTSTPQQQSHGHSTSRPVNKPQWHKPNESDPRLAHYPSQGFSTEEDEDEQVSAV

Target ORF information:

RefSeq Version	XM_011574273.1
Organism	Aquila chrysaetos canadensis
Definition	Aquila chrysaetos canadensis Rap guanine nucleotide exchange factor (GEF) 2 (RAPGEF2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011574273.1

ORF Insert Sequence:

ATGAAACCAC TTGCAATCCC AGCTAACCAT GGAGTTATGG GCCAGCAGGA GAAACACTCA 
CTTCCTGCAG ATTTCACAAA GCTGCACCTT ACTGACAGTC TCCACCCACA GGTGACCCAC 
GTTTCATCTA GTCACTCAGG ATGTAGCATC ACTAGTGATT CAGGAAGCAG CAGCCTTTCT 
GATATCTACC AGGCTACAGA AAGTGAGGCT GGTGATATGG ATCTTAGTGG GTTGCCAGAG 
ACAGCAGTGG ATTCTGAGGA TGATGATGAT GAAGAAGACA TTGAAAGGGC ATCGGATCCT 
TTGATGAGTA GGGATATTGT TAGAGACTGC TTGGAGAAAG ACCCAATAGA CAGAACAGAT 
GATGACATTG AACAACTACT GGAATTCATG CATCAATTGC CTGCTTTCGC CAACATGACA 
ATGTCAGTGA GGAGGGAGCT CTGTGCTGTG ATGGTGTTTG CAGTTGTGGA GAGAGCAGGA 
ACTATTGTAC TGAACGATGG GGAAGAGCTG GATTCCTGGT CAGTCATTTT GAATGGCTCT 
GTGGAGGTGA CGTACCCTGA TGGAAGAACA GAGATACTCT GCATGGGGAA CAGTTTTGGT 
GTTTCCCCTA CCATGGAAAA GGAATATATG AAAGGAGTGA TGAGAACCAA AGTGGATGAC 
TGCCAGTTTG TTTGTATAGC CCAGCAAGAT TATTGCCGCA TCCTCAATCA AGTAGAAAAG 
AACATGCAGA AGGTAGAAGA GGAAGGGGAG ATTGTGATGG TGAAGGAGCA CAGGGAGCTT 
GATCGCACTG GAACAAGAAA AGGTCACATT GTCATCAAGG GAACAGCTGA AAGGTTAACA 
ATGCATTTGG TGGAAGAGCA CTCTGTGGTA GACCCAACAT TTATAGAGGA CTTCCTGTTG 
ACATATCGGA CCTTTCTTTC CAGCCCGATG GAAGTGGGCA AGAAGTTGTT GGAGTGGTTC 
AATGACCCCA GCCTCAGGGA TAAGGTTACA CGGGTAGTAT TGTTGTGGGT GAACAATCAC 
TTCAATGATT TTGAAGGAGA TCCTGCTATG ACTCGATTTT TGGAAGAATT TGAGAACAAT 
TTGGAGAGGG AGAAAATGGG TGGACATTTG AGGCTGTTAA ATATTGCTTG TGCTGCTAAA 
GCTAAACGAA GATTGATAAC CTTAACAAAG CCATCTCGAG AAGCCCCTTT GCCTTTTATC 
TTGCTGGGAG GGTCAGAAAA GGGATTTGGA ATCTTTGTTG ACAGTGTAGA TTTTGGTAGC 
AAAGCCACAG AAGCAGGCTT GAAACGTGGA GACCAGATAC TGGAAGTGAA TGGTCAAAAC 
TTTGAAAACA TTCAGCTGTC AAAAGCCATG GAAATCCTTA GAAATAACAC TCATCTGTCT 
ATCACTGTGA AAACAAATTT ATTTGTTTTT AAAGAACTCT TAACACGGTT GTCAGAGGAA 
AAAAGAAATG GTGCTCCCCA CCTGCCTAAA ATTGGTGATA TTAAAAAGGC GAGTCGTTAT 
TCCATCCCAG ACCTTGCTGT TGATGTGGAG CAGGTAATAG GATTAGAAAA AGTAAATAAG 
AAAAGTAAAG CCAACACAGT TGGGGGAAGA AATAAATTAA AGAAGATACT TGACAAGACT 
CGAATCAGTA TTCTGCCTCA GAAACCGTAC AATGACATTG GAATTGGCCA GTCTCAGGAT 
GACAGCATTG TGGGACTGAG GCAAACAAAG CACATTCCTC CTGCTTTACC TGTCAGCGGA 
ACCCTGTCAT CCAGTAATCC CGATCTACTG CAGTCTCATC ACCGCATCTT AGACTTCAAT 
ACTACTCCGG ACTTACCAGA TCAAGTTCTG AGGGTTTTCA AGGCAGATCA GCAAAGTCGC 
TACATCATGA TCAGTAAGGA CACAACAGCA AAAGAAGTGG TCATCCAGGC TATCAGGGAG 
TTTGCTCTCA CTGCAACCCC TGATGCATAT TCACTATGCG AAGTGTCTGT CACACCTGAG 
GGTGTGATCA AGCAAAGGAG GCTTCCTGAT CAGTTATCCA AGCTTGCTGA CAGGATACAG 
CTGAGTGGCA GGTATTACCT GAAGAACAAC ATGGAAACAG AAACTCTTTG TTCAGATGAA 
GATGCTCAAG AGTTACTAAG GGAAAGTCAA ATTTCCCTAT TACAGCTCAG TACTGTTGAG 
GTGGCTACCC AACTCTCCAT GAGAAACTTC GAGCTATTCC GTAATATTGA ACCCACGGAA 
TACATAGATG ACTTGTTTAA ACTCAAATCA AAAACAGGTT GCACTAATCT AAAAAAATTT 
GAAGAGGTGA TAAATCAAGA AACATTCTGG GTGGCTTCTG AGATTCTAAG AGAAACCAAC 
CAGCTGAAAA GGATGAAGAT CATTAAGCAT TTCATTAAGA TAGCACTACA CTGCAGAGAA 
TGCAAGAACT TCAACTCAAT GTTTGCCATC ATTAGTGGCC TGAATTTGGC ACCAGTTGCA 
AGGCTCCGAA CTACTTGGGA AAAGCTTCCA AGCAAGTATG AAAAACTGTT TCAAGATCTA 
CAGGACTTGT TTGATCCATC TAGGAATATG GCAAAATATC GTAATGTCCT TAACAGCCAA 
AACCTACAGC CCCCCATTAT ACCCCTGTTT CCTGTTATCA AAAAAGACCT TACATTCCTT 
CATGAAGGAA ATGATTCAAA AGTGGAGGGC TTGGTCAATT TTGAGAAGCT GAGAATGATT 
GCAAAGGAGA TACGCCATGT CGGTCGCATG GCATCTGTGA ACATGGATCC TGCTTTAATG 
TTTAGAACAA GGAAGAAGAA ATGGAGGAGT TTGGGGTCTC TCAGCCAGGG CAGTACAAAT 
GCAGCTGTGC TGGATGTCGC GCAAACGGGG GGTCATAAAA AGCGGGTCCG TCGCAGCTCC 
TTCCTTAATG CTAAAAAACT GTATGAAGAT GCTCAGATGG CACGGAAAGT GAAGCAGTAT 
CTCTCAAACT TAGATCTGGA AATGGATGAA GAGAGCCTCC AGACACTGTC TCTGCAGTGT 
GAGCCAGCCA CCAACACACT GCCGAAGAAT ACAGGAGACA AGCGATCTGG AAAGTCTGAA 
ACGTCACCGG TGGCTCCCCG GGCAGGCATC CAGCAGAAAG TGCAGCAGCA GCAGCAACAT 
AAAGTAAACC AAGCATTGCA GGTCCCTGCC GTATCTCTTT ATCCCTCTCG CAAGAAAGTG 
CCGGTCAAAG ACCTCCCACC ATTTGGCATT AACTCCCCAC AAGCTTTAAA GAAAATTCTT 
TCCTTATCTG AAGAAGGAAG TTTGGATCGT CACAAGAAAC AATCTGAAGA CACGGTTTCA 
AGTGCGTCTT CGCAGCTGTC TTCTCCTCCC ACTTCACCAC AGAGCTCTCC AAGGAAAGGC 
TATACTTTGG CTCCTAGCGG CACAGTGGAT AACTTTTCAG ATTCTGGTCA CAGCGAAATT 
TCTTCCAGAT CTAGTATTGT CAGCAATTCC TCTTTTGACT CTATGCCAGT CTCCCTGCAT 
GATGAGAGGA GACAGAGACA TTCTGTCAGC ATCGTGGAGA CAAATCTTGG TGTGGGAAGG 
ATTGATAGAA GAATCATGAT TGAACCAGAT CAATACAGCT TAGGCTCATA TGCACCACTG 
TCAGAGACCA GAGGCCTGTA TGCTGGAGCA ACTGTGCTTT CTTCTCCTAG TACAGAAGAA 
CTGTCACAGG ATCAGGGGGA CAGAGCTTCG CTCGATGCAG CTGACAGTGG CCGTGGTAGC 
TGGACTTCAT GTTCGAGCGG TTCTCATGAC AACATACAAA CAATACAGCA CCAGAGAAGC 
TGGGAGACTC TGCCTTTTGG ACATGCTCAT TTCGATAGTT CAGGCGATGG GGCAGGACTG 
TGGGCCTCGG GCAGTCATAT GGACCAGCTG ATGTTCCCTG ATCATGGCAC AAAGTATGGC 
AGGCAAAGTC AAGGTAGGGA GGGCCTTGAT CAAGCACAGT CCAGAGCAAG CTGGGCATCC 
TCGACAGGAT ACTGGGGAGA GGACTCGGAA GGTGATACAG GTACCATAAA GCGGAGGGGT 
GGGAAGGATG TTTCAATTGA AGCTGAAACC AGTAGCATAA CATCTGTACC AGCAGAGGAG 
TCAAAGCCAG CTCCCATGCC CACTCATATA GCAGTATCAT CAAGTAGTAC AAAGGGGCTT 
ATTGCACGAA AAGAGGGTCG ATACCGGGAA CCACCTCCAA CTCCTCCTGG TTACGTAGGA 
ATACCTATTG CTGAGTTTGC AGAAGGTGGT TCCCATCCAA CCAGAAAGCC TCCAGATTAC 
AACATAGCAC TTCAGAGGTC TAGAATGGTG GCGCGGACAT GTGAGACTCA TGGGACATCA 
ACTCCACAGC AGCAGTCACA CGGCCATTCA ACTAGCAGGC CTGTGAACAA ACCCCAGTGG 
CATAAACCAA ATGAGTCAGA TCCACGTCTT GCTCACTATC CATCTCAAGG GTTTTCCACA 
GAGGAAGATG AAGATGAACA AGTCTCTGCT GTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF2 ( XM_011574274.1 ) cDNA ORF clone, Aquila chrysaetos canadensis -> XP_011572576.1 Aquila chrysaetos canadensis Rap guanine nucleotide exchange factor (GEF) 2 (RAPGEF2), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OAq14198
Clone ID Related Accession (Same CDS sequence)	XM_011574274.1
Accession Version	XM_011574274.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4470bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-12
Organism	Aquila chrysaetos canadensis
Product	rap guanine nucleotide exchange factor 2 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011950881.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Aquila chrysaetos canadensis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTTCC TTGTGCGTTG CTATGCCAAC TGCCTGCAGC CATGGTCTTC CAAACTTCCT 
GCAGATTTCA CAAAGCTGCA CCTTACTGAC AGTCTCCACC CACAGGTGAC CCACGTTTCA 
TCTAGTCACT CAGGATGTAG CATCACTAGT GATTCAGGAA GCAGCAGCCT TTCTGATATC 
TACCAGGCTA CAGAAAGTGA GGCTGGTGAT ATGGATCTTA GTGGGTTGCC AGAGACAGCA 
GTGGATTCTG AGGATGATGA TGATGAAGAA GACATTGAAA GGGCATCGGA TCCTTTGATG 
AGTAGGGATA TTGTTAGAGA CTGCTTGGAG AAAGACCCAA TAGACAGAAC AGATGATGAC 
ATTGAACAAC TACTGGAATT CATGCATCAA TTGCCTGCTT TCGCCAACAT GACAATGTCA 
GTGAGGAGGG AGCTCTGTGC TGTGATGGTG TTTGCAGTTG TGGAGAGAGC AGGAACTATT 
GTACTGAACG ATGGGGAAGA GCTGGATTCC TGGTCAGTCA TTTTGAATGG CTCTGTGGAG 
GTGACGTACC CTGATGGAAG AACAGAGATA CTCTGCATGG GGAACAGTTT TGGTGTTTCC 
CCTACCATGG AAAAGGAATA TATGAAAGGA GTGATGAGAA CCAAAGTGGA TGACTGCCAG 
TTTGTTTGTA TAGCCCAGCA AGATTATTGC CGCATCCTCA ATCAAGTAGA AAAGAACATG 
CAGAAGGTAG AAGAGGAAGG GGAGATTGTG ATGGTGAAGG AGCACAGGGA GCTTGATCGC 
ACTGGAACAA GAAAAGGTCA CATTGTCATC AAGGGAACAG CTGAAAGGTT AACAATGCAT 
TTGGTGGAAG AGCACTCTGT GGTAGACCCA ACATTTATAG AGGACTTCCT GTTGACATAT 
CGGACCTTTC TTTCCAGCCC GATGGAAGTG GGCAAGAAGT TGTTGGAGTG GTTCAATGAC 
CCCAGCCTCA GGGATAAGGT TACACGGGTA GTATTGTTGT GGGTGAACAA TCACTTCAAT 
GATTTTGAAG GAGATCCTGC TATGACTCGA TTTTTGGAAG AATTTGAGAA CAATTTGGAG 
AGGGAGAAAA TGGGTGGACA TTTGAGGCTG TTAAATATTG CTTGTGCTGC TAAAGCTAAA 
CGAAGATTGA TAACCTTAAC AAAGCCATCT CGAGAAGCCC CTTTGCCTTT TATCTTGCTG 
GGAGGGTCAG AAAAGGGATT TGGAATCTTT GTTGACAGTG TAGATTTTGG TAGCAAAGCC 
ACAGAAGCAG GCTTGAAACG TGGAGACCAG ATACTGGAAG TGAATGGTCA AAACTTTGAA 
AACATTCAGC TGTCAAAAGC CATGGAAATC CTTAGAAATA ACACTCATCT GTCTATCACT 
GTGAAAACAA ATTTATTTGT TTTTAAAGAA CTCTTAACAC GGTTGTCAGA GGAAAAAAGA 
AATGGTGCTC CCCACCTGCC TAAAATTGGT GATATTAAAA AGGCGAGTCG TTATTCCATC 
CCAGACCTTG CTGTTGATGT GGAGCAGGTA ATAGGATTAG AAAAAGTAAA TAAGAAAAGT 
AAAGCCAACA CAGTTGGGGG AAGAAATAAA TTAAAGAAGA TACTTGACAA GACTCGAATC 
AGTATTCTGC CTCAGAAACC GTACAATGAC ATTGGAATTG GCCAGTCTCA GGATGACAGC 
ATTGTGGGAC TGAGGCAAAC AAAGCACATT CCTCCTGCTT TACCTGTCAG CGGAACCCTG 
TCATCCAGTA ATCCCGATCT ACTGCAGTCT CATCACCGCA TCTTAGACTT CAATACTACT 
CCGGACTTAC CAGATCAAGT TCTGAGGGTT TTCAAGGCAG ATCAGCAAAG TCGCTACATC 
ATGATCAGTA AGGACACAAC AGCAAAAGAA GTGGTCATCC AGGCTATCAG GGAGTTTGCT 
CTCACTGCAA CCCCTGATGC ATATTCACTA TGCGAAGTGT CTGTCACACC TGAGGGTGTG 
ATCAAGCAAA GGAGGCTTCC TGATCAGTTA TCCAAGCTTG CTGACAGGAT ACAGCTGAGT 
GGCAGGTATT ACCTGAAGAA CAACATGGAA ACAGAAACTC TTTGTTCAGA TGAAGATGCT 
CAAGAGTTAC TAAGGGAAAG TCAAATTTCC CTATTACAGC TCAGTACTGT TGAGGTGGCT 
ACCCAACTCT CCATGAGAAA CTTCGAGCTA TTCCGTAATA TTGAACCCAC GGAATACATA 
GATGACTTGT TTAAACTCAA ATCAAAAACA GGTTGCACTA ATCTAAAAAA ATTTGAAGAG 
GTGATAAATC AAGAAACATT CTGGGTGGCT TCTGAGATTC TAAGAGAAAC CAACCAGCTG 
AAAAGGATGA AGATCATTAA GCATTTCATT AAGATAGCAC TACACTGCAG AGAATGCAAG 
AACTTCAACT CAATGTTTGC CATCATTAGT GGCCTGAATT TGGCACCAGT TGCAAGGCTC 
CGAACTACTT GGGAAAAGCT TCCAAGCAAG TATGAAAAAC TGTTTCAAGA TCTACAGGAC 
TTGTTTGATC CATCTAGGAA TATGGCAAAA TATCGTAATG TCCTTAACAG CCAAAACCTA 
CAGCCCCCCA TTATACCCCT GTTTCCTGTT ATCAAAAAAG ACCTTACATT CCTTCATGAA 
GGAAATGATT CAAAAGTGGA GGGCTTGGTC AATTTTGAGA AGCTGAGAAT GATTGCAAAG 
GAGATACGCC ATGTCGGTCG CATGGCATCT GTGAACATGG ATCCTGCTTT AATGTTTAGA 
ACAAGGAAGA AGAAATGGAG GAGTTTGGGG TCTCTCAGCC AGGGCAGTAC AAATGCAGCT 
GTGCTGGATG TCGCGCAAAC GGGGGGTCAT AAAAAGCGGG TCCGTCGCAG CTCCTTCCTT 
AATGCTAAAA AACTGTATGA AGATGCTCAG ATGGCACGGA AAGTGAAGCA GTATCTCTCA 
AACTTAGATC TGGAAATGGA TGAAGAGAGC CTCCAGACAC TGTCTCTGCA GTGTGAGCCA 
GCCACCAACA CACTGCCGAA GAATACAGGA GACAAGCGAT CTGGAAAGTC TGAAACGTCA 
CCGGTGGCTC CCCGGGCAGG CATCCAGCAG AAAGTGCAGC AGCAGCAGCA ACATAAAGTA 
AACCAAGCAT TGCAGGTCCC TGCCGTATCT CTTTATCCCT CTCGCAAGAA AGTGCCGGTC 
AAAGACCTCC CACCATTTGG CATTAACTCC CCACAAGCTT TAAAGAAAAT TCTTTCCTTA 
TCTGAAGAAG GAAGTTTGGA TCGTCACAAG AAACAATCTG AAGACACGGT TTCAAGTGCG 
TCTTCGCAGC TGTCTTCTCC TCCCACTTCA CCACAGAGCT CTCCAAGGAA AGGCTATACT 
TTGGCTCCTA GCGGCACAGT GGATAACTTT TCAGATTCTG GTCACAGCGA AATTTCTTCC 
AGATCTAGTA TTGTCAGCAA TTCCTCTTTT GACTCTATGC CAGTCTCCCT GCATGATGAG 
AGGAGACAGA GACATTCTGT CAGCATCGTG GAGACAAATC TTGGTGTGGG AAGGATTGAT 
AGAAGAATCA TGATTGAACC AGATCAATAC AGCTTAGGCT CATATGCACC ACTGTCAGAG 
ACCAGAGGCC TGTATGCTGG AGCAACTGTG CTTTCTTCTC CTAGTACAGA AGAACTGTCA 
CAGGATCAGG GGGACAGAGC TTCGCTCGAT GCAGCTGACA GTGGCCGTGG TAGCTGGACT 
TCATGTTCGA GCGGTTCTCA TGACAACATA CAAACAATAC AGCACCAGAG AAGCTGGGAG 
ACTCTGCCTT TTGGACATGC TCATTTCGAT AGTTCAGGCG ATGGGGCAGG ACTGTGGGCC 
TCGGGCAGTC ATATGGACCA GCTGATGTTC CCTGATCATG GCACAAAGTA TGGCAGGCAA 
AGTCAAGGTA GGGAGGGCCT TGATCAAGCA CAGTCCAGAG CAAGCTGGGC ATCCTCGACA 
GGATACTGGG GAGAGGACTC GGAAGGTGAT ACAGGTACCA TAAAGCGGAG GGGTGGGAAG 
GATGTTTCAA TTGAAGCTGA AACCAGTAGC ATAACATCTG TACCAGCAGA GGAGTCAAAG 
CCAGCTCCCA TGCCCACTCA TATAGCAGTA TCATCAAGTA GTACAAAGGG GCTTATTGCA 
CGAAAAGAGG GTCGATACCG GGAACCACCT CCAACTCCTC CTGGTTACGT AGGAATACCT 
ATTGCTGAGT TTGCAGAAGG TGGTTCCCAT CCAACCAGAA AGCCTCCAGA TTACAACATA 
GCACTTCAGA GGTCTAGAAT GGTGGCGCGG ACATGTGAGA CTCATGGGAC ATCAACTCCA 
CAGCAGCAGT CACACGGCCA TTCAACTAGC AGGCCTGTGA ACAAACCCCA GTGGCATAAA 
CCAAATGAGT CAGATCCACG TCTTGCTCAC TATCCATCTC AAGGGTTTTC CACAGAGGAA 
GATGAAGATG AACAAGTCTC TGCTGTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011572576.1
CDS	53..4522
Translation	MAFLVRCYANCLQPWSSKLPADFTKLHLTDSLHPQVTHVSSSHSGCSITSDSGSSSLSDIYQATESEAGDMDLSGLPETAVDSEDDDDEEDIERASDPLMSRDIVRDCLEKDPIDRTDDDIEQLLEFMHQLPAFANMTMSVRRELCAVMVFAVVERAGTIVLNDGEELDSWSVILNGSVEVTYPDGRTEILCMGNSFGVSPTMEKEYMKGVMRTKVDDCQFVCIAQQDYCRILNQVEKNMQKVEEEGEIVMVKEHRELDRTGTRKGHIVIKGTAERLTMHLVEEHSVVDPTFIEDFLLTYRTFLSSPMEVGKKLLEWFNDPSLRDKVTRVVLLWVNNHFNDFEGDPAMTRFLEEFENNLEREKMGGHLRLLNIACAAKAKRRLITLTKPSREAPLPFILLGGSEKGFGIFVDSVDFGSKATEAGLKRGDQILEVNGQNFENIQLSKAMEILRNNTHLSITVKTNLFVFKELLTRLSEEKRNGAPHLPKIGDIKKASRYSIPDLAVDVEQVIGLEKVNKKSKANTVGGRNKLKKILDKTRISILPQKPYNDIGIGQSQDDSIVGLRQTKHIPPALPVSGTLSSSNPDLLQSHHRILDFNTTPDLPDQVLRVFKADQQSRYIMISKDTTAKEVVIQAIREFALTATPDAYSLCEVSVTPEGVIKQRRLPDQLSKLADRIQLSGRYYLKNNMETETLCSDEDAQELLRESQISLLQLSTVEVATQLSMRNFELFRNIEPTEYIDDLFKLKSKTGCTNLKKFEEVINQETFWVASEILRETNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLAPVARLRTTWEKLPSKYEKLFQDLQDLFDPSRNMAKYRNVLNSQNLQPPIIPLFPVIKKDLTFLHEGNDSKVEGLVNFEKLRMIAKEIRHVGRMASVNMDPALMFRTRKKKWRSLGSLSQGSTNAAVLDVAQTGGHKKRVRRSSFLNAKKLYEDAQMARKVKQYLSNLDLEMDEESLQTLSLQCEPATNTLPKNTGDKRSGKSETSPVAPRAGIQQKVQQQQQHKVNQALQVPAVSLYPSRKKVPVKDLPPFGINSPQALKKILSLSEEGSLDRHKKQSEDTVSSASSQLSSPPTSPQSSPRKGYTLAPSGTVDNFSDSGHSEISSRSSIVSNSSFDSMPVSLHDERRQRHSVSIVETNLGVGRIDRRIMIEPDQYSLGSYAPLSETRGLYAGATVLSSPSTEELSQDQGDRASLDAADSGRGSWTSCSSGSHDNIQTIQHQRSWETLPFGHAHFDSSGDGAGLWASGSHMDQLMFPDHGTKYGRQSQGREGLDQAQSRASWASSTGYWGEDSEGDTGTIKRRGGKDVSIEAETSSITSVPAEESKPAPMPTHIAVSSSSTKGLIARKEGRYREPPPTPPGYVGIPIAEFAEGGSHPTRKPPDYNIALQRSRMVARTCETHGTSTPQQQSHGHSTSRPVNKPQWHKPNESDPRLAHYPSQGFSTEEDEDEQVSAV

Target ORF information:

RefSeq Version	XM_011574274.1
Organism	Aquila chrysaetos canadensis
Definition	Aquila chrysaetos canadensis Rap guanine nucleotide exchange factor (GEF) 2 (RAPGEF2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011574274.1

ORF Insert Sequence:

ATGGCTTTCC TTGTGCGTTG CTATGCCAAC TGCCTGCAGC CATGGTCTTC CAAACTTCCT 
GCAGATTTCA CAAAGCTGCA CCTTACTGAC AGTCTCCACC CACAGGTGAC CCACGTTTCA 
TCTAGTCACT CAGGATGTAG CATCACTAGT GATTCAGGAA GCAGCAGCCT TTCTGATATC 
TACCAGGCTA CAGAAAGTGA GGCTGGTGAT ATGGATCTTA GTGGGTTGCC AGAGACAGCA 
GTGGATTCTG AGGATGATGA TGATGAAGAA GACATTGAAA GGGCATCGGA TCCTTTGATG 
AGTAGGGATA TTGTTAGAGA CTGCTTGGAG AAAGACCCAA TAGACAGAAC AGATGATGAC 
ATTGAACAAC TACTGGAATT CATGCATCAA TTGCCTGCTT TCGCCAACAT GACAATGTCA 
GTGAGGAGGG AGCTCTGTGC TGTGATGGTG TTTGCAGTTG TGGAGAGAGC AGGAACTATT 
GTACTGAACG ATGGGGAAGA GCTGGATTCC TGGTCAGTCA TTTTGAATGG CTCTGTGGAG 
GTGACGTACC CTGATGGAAG AACAGAGATA CTCTGCATGG GGAACAGTTT TGGTGTTTCC 
CCTACCATGG AAAAGGAATA TATGAAAGGA GTGATGAGAA CCAAAGTGGA TGACTGCCAG 
TTTGTTTGTA TAGCCCAGCA AGATTATTGC CGCATCCTCA ATCAAGTAGA AAAGAACATG 
CAGAAGGTAG AAGAGGAAGG GGAGATTGTG ATGGTGAAGG AGCACAGGGA GCTTGATCGC 
ACTGGAACAA GAAAAGGTCA CATTGTCATC AAGGGAACAG CTGAAAGGTT AACAATGCAT 
TTGGTGGAAG AGCACTCTGT GGTAGACCCA ACATTTATAG AGGACTTCCT GTTGACATAT 
CGGACCTTTC TTTCCAGCCC GATGGAAGTG GGCAAGAAGT TGTTGGAGTG GTTCAATGAC 
CCCAGCCTCA GGGATAAGGT TACACGGGTA GTATTGTTGT GGGTGAACAA TCACTTCAAT 
GATTTTGAAG GAGATCCTGC TATGACTCGA TTTTTGGAAG AATTTGAGAA CAATTTGGAG 
AGGGAGAAAA TGGGTGGACA TTTGAGGCTG TTAAATATTG CTTGTGCTGC TAAAGCTAAA 
CGAAGATTGA TAACCTTAAC AAAGCCATCT CGAGAAGCCC CTTTGCCTTT TATCTTGCTG 
GGAGGGTCAG AAAAGGGATT TGGAATCTTT GTTGACAGTG TAGATTTTGG TAGCAAAGCC 
ACAGAAGCAG GCTTGAAACG TGGAGACCAG ATACTGGAAG TGAATGGTCA AAACTTTGAA 
AACATTCAGC TGTCAAAAGC CATGGAAATC CTTAGAAATA ACACTCATCT GTCTATCACT 
GTGAAAACAA ATTTATTTGT TTTTAAAGAA CTCTTAACAC GGTTGTCAGA GGAAAAAAGA 
AATGGTGCTC CCCACCTGCC TAAAATTGGT GATATTAAAA AGGCGAGTCG TTATTCCATC 
CCAGACCTTG CTGTTGATGT GGAGCAGGTA ATAGGATTAG AAAAAGTAAA TAAGAAAAGT 
AAAGCCAACA CAGTTGGGGG AAGAAATAAA TTAAAGAAGA TACTTGACAA GACTCGAATC 
AGTATTCTGC CTCAGAAACC GTACAATGAC ATTGGAATTG GCCAGTCTCA GGATGACAGC 
ATTGTGGGAC TGAGGCAAAC AAAGCACATT CCTCCTGCTT TACCTGTCAG CGGAACCCTG 
TCATCCAGTA ATCCCGATCT ACTGCAGTCT CATCACCGCA TCTTAGACTT CAATACTACT 
CCGGACTTAC CAGATCAAGT TCTGAGGGTT TTCAAGGCAG ATCAGCAAAG TCGCTACATC 
ATGATCAGTA AGGACACAAC AGCAAAAGAA GTGGTCATCC AGGCTATCAG GGAGTTTGCT 
CTCACTGCAA CCCCTGATGC ATATTCACTA TGCGAAGTGT CTGTCACACC TGAGGGTGTG 
ATCAAGCAAA GGAGGCTTCC TGATCAGTTA TCCAAGCTTG CTGACAGGAT ACAGCTGAGT 
GGCAGGTATT ACCTGAAGAA CAACATGGAA ACAGAAACTC TTTGTTCAGA TGAAGATGCT 
CAAGAGTTAC TAAGGGAAAG TCAAATTTCC CTATTACAGC TCAGTACTGT TGAGGTGGCT 
ACCCAACTCT CCATGAGAAA CTTCGAGCTA TTCCGTAATA TTGAACCCAC GGAATACATA 
GATGACTTGT TTAAACTCAA ATCAAAAACA GGTTGCACTA ATCTAAAAAA ATTTGAAGAG 
GTGATAAATC AAGAAACATT CTGGGTGGCT TCTGAGATTC TAAGAGAAAC CAACCAGCTG 
AAAAGGATGA AGATCATTAA GCATTTCATT AAGATAGCAC TACACTGCAG AGAATGCAAG 
AACTTCAACT CAATGTTTGC CATCATTAGT GGCCTGAATT TGGCACCAGT TGCAAGGCTC 
CGAACTACTT GGGAAAAGCT TCCAAGCAAG TATGAAAAAC TGTTTCAAGA TCTACAGGAC 
TTGTTTGATC CATCTAGGAA TATGGCAAAA TATCGTAATG TCCTTAACAG CCAAAACCTA 
CAGCCCCCCA TTATACCCCT GTTTCCTGTT ATCAAAAAAG ACCTTACATT CCTTCATGAA 
GGAAATGATT CAAAAGTGGA GGGCTTGGTC AATTTTGAGA AGCTGAGAAT GATTGCAAAG 
GAGATACGCC ATGTCGGTCG CATGGCATCT GTGAACATGG ATCCTGCTTT AATGTTTAGA 
ACAAGGAAGA AGAAATGGAG GAGTTTGGGG TCTCTCAGCC AGGGCAGTAC AAATGCAGCT 
GTGCTGGATG TCGCGCAAAC GGGGGGTCAT AAAAAGCGGG TCCGTCGCAG CTCCTTCCTT 
AATGCTAAAA AACTGTATGA AGATGCTCAG ATGGCACGGA AAGTGAAGCA GTATCTCTCA 
AACTTAGATC TGGAAATGGA TGAAGAGAGC CTCCAGACAC TGTCTCTGCA GTGTGAGCCA 
GCCACCAACA CACTGCCGAA GAATACAGGA GACAAGCGAT CTGGAAAGTC TGAAACGTCA 
CCGGTGGCTC CCCGGGCAGG CATCCAGCAG AAAGTGCAGC AGCAGCAGCA ACATAAAGTA 
AACCAAGCAT TGCAGGTCCC TGCCGTATCT CTTTATCCCT CTCGCAAGAA AGTGCCGGTC 
AAAGACCTCC CACCATTTGG CATTAACTCC CCACAAGCTT TAAAGAAAAT TCTTTCCTTA 
TCTGAAGAAG GAAGTTTGGA TCGTCACAAG AAACAATCTG AAGACACGGT TTCAAGTGCG 
TCTTCGCAGC TGTCTTCTCC TCCCACTTCA CCACAGAGCT CTCCAAGGAA AGGCTATACT 
TTGGCTCCTA GCGGCACAGT GGATAACTTT TCAGATTCTG GTCACAGCGA AATTTCTTCC 
AGATCTAGTA TTGTCAGCAA TTCCTCTTTT GACTCTATGC CAGTCTCCCT GCATGATGAG 
AGGAGACAGA GACATTCTGT CAGCATCGTG GAGACAAATC TTGGTGTGGG AAGGATTGAT 
AGAAGAATCA TGATTGAACC AGATCAATAC AGCTTAGGCT CATATGCACC ACTGTCAGAG 
ACCAGAGGCC TGTATGCTGG AGCAACTGTG CTTTCTTCTC CTAGTACAGA AGAACTGTCA 
CAGGATCAGG GGGACAGAGC TTCGCTCGAT GCAGCTGACA GTGGCCGTGG TAGCTGGACT 
TCATGTTCGA GCGGTTCTCA TGACAACATA CAAACAATAC AGCACCAGAG AAGCTGGGAG 
ACTCTGCCTT TTGGACATGC TCATTTCGAT AGTTCAGGCG ATGGGGCAGG ACTGTGGGCC 
TCGGGCAGTC ATATGGACCA GCTGATGTTC CCTGATCATG GCACAAAGTA TGGCAGGCAA 
AGTCAAGGTA GGGAGGGCCT TGATCAAGCA CAGTCCAGAG CAAGCTGGGC ATCCTCGACA 
GGATACTGGG GAGAGGACTC GGAAGGTGAT ACAGGTACCA TAAAGCGGAG GGGTGGGAAG 
GATGTTTCAA TTGAAGCTGA AACCAGTAGC ATAACATCTG TACCAGCAGA GGAGTCAAAG 
CCAGCTCCCA TGCCCACTCA TATAGCAGTA TCATCAAGTA GTACAAAGGG GCTTATTGCA 
CGAAAAGAGG GTCGATACCG GGAACCACCT CCAACTCCTC CTGGTTACGT AGGAATACCT 
ATTGCTGAGT TTGCAGAAGG TGGTTCCCAT CCAACCAGAA AGCCTCCAGA TTACAACATA 
GCACTTCAGA GGTCTAGAAT GGTGGCGCGG ACATGTGAGA CTCATGGGAC ATCAACTCCA 
CAGCAGCAGT CACACGGCCA TTCAACTAGC AGGCCTGTGA ACAAACCCCA GTGGCATAAA 
CCAAATGAGT CAGATCCACG TCTTGCTCAC TATCCATCTC AAGGGTTTTC CACAGAGGAA 
GATGAAGATG AACAAGTCTC TGCTGTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF2 ( XM_011574276.1 ) cDNA ORF clone, Aquila chrysaetos canadensis -> XP_011572578.1 Aquila chrysaetos canadensis Rap guanine nucleotide exchange factor (GEF) 2 (RAPGEF2), transcript variant X6, mRNA.

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CloneID	OAq14199
Clone ID Related Accession (Same CDS sequence)	XM_011574276.1
Accession Version	XM_011574276.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4056bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-12
Organism	Aquila chrysaetos canadensis
Product	rap guanine nucleotide exchange factor 2 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011950881.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Aquila chrysaetos canadensis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGCCT TGTGTAATCT GAGAGAGCAT CAGCTAAGAC TGATGTGTGA AACTGTTAGA 
TATGAAAGGC ATGAAGCAAA TGAAGTTCTG TACTACCCTG ATGATATTGG TACCTGTTGG 
TATATCCTGC TGTCTGGTTC AGTCTTCATT AAAGAATCTA TGTTTCTTCC AAGGAGCAGT 
TTTGGCAAGC GTTCTGCAGG AAGCTTCAGG CGTGGCTGTG AATGCATTGT TTTAGAGCCA 
TCTGAAATGA TTGTGGTTGA TTACATGGAT GAAAATGAGG AGTATTTTCA GCGTCAAGCT 
TCTCATAGGC AATCTCGAAG AAGATTTAGG AAAATCAACC AAAAAGGGGA GCGACAAACA 
ATTATTGATA CTGTTGACCC TTATCCTGTG GGGAAGCCAC CTCTACCTAG AGGTTACCAT 
ACGGAATGCA CTAAACCTCA GCTTCCTGCA GATTTCACAA AGCTGCACCT TACTGACAGT 
CTCCACCCAC AGGTGACCCA CGTTTCATCT AGTCACTCAG GATGTAGCAT CACTAGTGAT 
TCAGGAAGCA GCAGCCTTTC TGATATCTAC CAGGCTACAG AAAGTGAGGC TGGTGATATG 
GATCTTAGTG GGTTGCCAGA GACAGCAGTG GATTCTGAGG ATGATGATGA TGAAGAAGAC 
ATTGAAAGGG CATCGGATCC TTTGATGAGT AGGGATATTG TTAGAGACTG CTTGGAGAAA 
GACCCAATAG ACAGAACAGA TGATGACATT GAACAACTAC TGGAATTCAT GCATCAATTG 
CCTGCTTTCG CCAACATGAC AATGTCAGTG AGGAGGGAGC TCTGTGCTGT GATGGTGTTT 
GCAGTTGTGG AGAGAGCAGG AACTATTGTA CTGAACGATG GGGAAGAGCT GGATTCCTGG 
TCAGTCATTT TGAATGGCTC TGTGGAGGTG ACGTACCCTG ATGGAAGAAC AGAGATACTC 
TGCATGGGGA ACAGTTTTGG TGTTTCCCCT ACCATGGAAA AGGAATATAT GAAAGGAGTG 
ATGAGAACCA AAGTGGATGA CTGCCAGTTT GTTTGTATAG CCCAGCAAGA TTATTGCCGC 
ATCCTCAATC AAGTAGAAAA GAACATGCAG AAGGTAGAAG AGGAAGGGGA GATTGTGATG 
GTGAAGGAGC ACAGGGAGCT TGATCGCACT GGAACAAGAA AAGGTCACAT TGTCATCAAG 
GGAACAGCTG AAAGGTTAAC AATGCATTTG GTGGAAGAGC ACTCTGTGGT AGACCCAACA 
TTTATAGAGG ACTTCCTGTT GACATATCGG ACCTTTCTTT CCAGCCCGAT GGAAGTGGGC 
AAGAAGTTGT TGGAGTGGTT CAATGACCCC AGCCTCAGGG ATAAGGTTAC ACGGGTAGTA 
TTGTTGTGGG TGAACAATCA CTTCAATGAT TTTGAAGGAG ATCCTGCTAT GACTCGATTT 
TTGGAAGAAT TTGAGAACAA TTTGGAGAGG GAGAAAATGG GTGGACATTT GAGGCTGTTA 
AATATTGCTT GTGCTGCTAA AGCTAAACGA AGATTGATAA CCTTAACAAA GCCATCTCGA 
GAAGCCCCTT TGCCTTTTAT CTTGCTGGGA GGGTCAGAAA AGGGATTTGG AATCTTTGTT 
GACAGTGTAG ATTTTGGTAG CAAAGCCACA GAAGCAGGCT TGAAACGTGG AGACCAGATA 
CTGGAAGTGA ATGGTCAAAA CTTTGAAAAC ATTCAGCTGT CAAAAGCCAT GGAAATCCTT 
AGAAATAACA CTCATCTGTC TATCACTGTG AAAACAAATT TATTTGTTTT TAAAGAACTC 
TTAACACGGT TGTCAGAGGA AAAAAGAAAT GGTGCTCCCC ACCTGCCTAA AATTGGTGAT 
ATTAAAAAGG CGAGTCGTTA TTCCATCCCA GACCTTGCTG TTGATGTGGA GCAGGTAATA 
GGATTAGAAA AAGTAAATAA GAAAAGTAAA GCCAACACAG TTGGGGGAAG AAATAAATTA 
AAGAAGATAC TTGACAAGAC TCGAATCAGT ATTCTGCCTC AGAAACCGTA CAATGACATT 
GGAATTGGCC AGTCTCAGGA TGACAGCATT GTGGGACTGA GGCAAACAAA GCACATTCCT 
CCTGCTTTAC CTGTCAGCGG AACCCTGTCA TCCAGTAATC CCGATCTACT GCAGTCTCAT 
CACCGCATCT TAGACTTCAA TACTACTCCG GACTTACCAG ATCAAGTTCT GAGGGTTTTC 
AAGGCAGATC AGCAAAGTCG CTACATCATG ATCAGTAAGG ACACAACAGC AAAAGAAGTG 
GTCATCCAGG CTATCAGGGA GTTTGCTCTC ACTGCAACCC CTGATGCATA TTCACTATGC 
GAAGTGTCTG TCACACCTGA GGGTGTGATC AAGCAAAGGA GGCTTCCTGA TCAGTTATCC 
AAGCTTGCTG ACAGGATACA GCTGAGTGGC AGGTATTACC TGAAGAACAA CATGGAAACA 
GAAACTCTTT GTTCAGATGA AGATGCTCAA GAGTTACTAA GGGAAAGTCA AATTTCCCTA 
TTACAGCTCA GTACTGTTGA GGTGGCTACC CAACTCTCCA TGAGAAACTT CGAGCTATTC 
CGTAATATTG AACCCACGGA ATACATAGAT GACTTGTTTA AACTCAAATC AAAAACAGGT 
TGCACTAATC TAAAAAAATT TGAAGAGGTG ATAAATCAAG AAACATTCTG GGTGGCTTCT 
GAGATTCTAA GAGAAACCAA CCAGCTGAAA AGGATGAAGA TCATTAAGCA TTTCATTAAG 
ATAGCACTAC ACTGCAGAGA ATGCAAGAAC TTCAACTCAA TGTTTGCCAT CATTAGTGGC 
CTGAATTTGG CACCAGTTGC AAGGCTCCGA ACTACTTGGG AAAAGCTTCC AAGCAAGTAT 
GAAAAACTGT TTCAAGATCT ACAGGACTTG TTTGATCCAT CTAGGAATAT GGCAAAATAT 
CGTAATGTCC TTAACAGCCA AAACCTACAG CCCCCCATTA TACCCCTGTT TCCTGTTATC 
AAAAAAGACC TTACATTCCT TCATGAAGGA AATGATTCAA AAGTGGAGGG CTTGGTCAAT 
TTTGAGAAGC TGAGAATGAT TGCAAAGGAG ATACGCCATG TCGGTCGCAT GGCATCTGTG 
AACATGGATC CTGCTTTAAT GTTTAGAACA AGGAAGAAGA AATGGAGGAG TTTGGGGTCT 
CTCAGCCAGG GCAGTACAAA TGCAGCTGTG CTGGATGTCG CGCAAACGGG GGGTCATAAA 
AAGCGGGTCC GTCGCAGCTC CTTCCTTAAT GCTAAAAAAC TGTATGAAGA TGCTCAGATG 
GCACGGAAAG TGAAGCAGTA TCTCTCAAAC TTAGATCTGG AAATGGATGA AGAGAGCCTC 
CAGACACTGT CTCTGCAGTG TGAGCCAGCC ACCAACACAC TGCCGAAGAA TACAGGAGAC 
AAGCGATCTG GAAAGTCTGA AACGTCACCG GTGGCTCCCC GGGCAGGCAT CCAGCAGAAA 
GTGCAGCAGC AGCAGCAACA TAAAGTAAAC CAAGCATTGC AGGTCCCTGC CGTATCTCTT 
TATCCCTCTC GCAAGAAAGT GCCGGTCAAA GACCTCCCAC CATTTGGCAT TAACTCCCCA 
CAAGCTTTAA AGAAAATTCT TTCCTTATCT GAAGAAGGAA GTTTGGATCG TCACAAGAAA 
CAATCTGAAG ACACGGTTTC AAGTGCGTCT TCGCAGCTGT CTTCTCCTCC CACTTCACCA 
CAGAGCTCTC CAAGGAAAGG TGGCAGTGCT GATCATTTGA GGCCTTATGG ATCCAGACTA 
ACAGAGCTAA CCAGTTGGTC CTCTCCTCTT GGAAGTGAGA ACAGTAACAA GAATAACAAT 
TCACCAAAGA TCTCTGGGAT AGGTGGGGCT TATGGAAACA AAATGGGAAA AAGTAAGTCC 
AATTGGATAA GCAGAGATCT GAAAGTGGGA GAGATGAATA TGTCAAGAAA GTGCAAATGC 
CTATCCAAAA TCTCTATTGA AGTGATATTT ACTAAGGTCA GATGGAGGAG AATGTTATGT 
AGAGAAGATT TAGATTATCA CCTCGAGTCC TTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011572578.1
CDS	30..4085
Translation	MEALCNLREHQLRLMCETVRYERHEANEVLYYPDDIGTCWYILLSGSVFIKESMFLPRSSFGKRSAGSFRRGCECIVLEPSEMIVVDYMDENEEYFQRQASHRQSRRRFRKINQKGERQTIIDTVDPYPVGKPPLPRGYHTECTKPQLPADFTKLHLTDSLHPQVTHVSSSHSGCSITSDSGSSSLSDIYQATESEAGDMDLSGLPETAVDSEDDDDEEDIERASDPLMSRDIVRDCLEKDPIDRTDDDIEQLLEFMHQLPAFANMTMSVRRELCAVMVFAVVERAGTIVLNDGEELDSWSVILNGSVEVTYPDGRTEILCMGNSFGVSPTMEKEYMKGVMRTKVDDCQFVCIAQQDYCRILNQVEKNMQKVEEEGEIVMVKEHRELDRTGTRKGHIVIKGTAERLTMHLVEEHSVVDPTFIEDFLLTYRTFLSSPMEVGKKLLEWFNDPSLRDKVTRVVLLWVNNHFNDFEGDPAMTRFLEEFENNLEREKMGGHLRLLNIACAAKAKRRLITLTKPSREAPLPFILLGGSEKGFGIFVDSVDFGSKATEAGLKRGDQILEVNGQNFENIQLSKAMEILRNNTHLSITVKTNLFVFKELLTRLSEEKRNGAPHLPKIGDIKKASRYSIPDLAVDVEQVIGLEKVNKKSKANTVGGRNKLKKILDKTRISILPQKPYNDIGIGQSQDDSIVGLRQTKHIPPALPVSGTLSSSNPDLLQSHHRILDFNTTPDLPDQVLRVFKADQQSRYIMISKDTTAKEVVIQAIREFALTATPDAYSLCEVSVTPEGVIKQRRLPDQLSKLADRIQLSGRYYLKNNMETETLCSDEDAQELLRESQISLLQLSTVEVATQLSMRNFELFRNIEPTEYIDDLFKLKSKTGCTNLKKFEEVINQETFWVASEILRETNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLAPVARLRTTWEKLPSKYEKLFQDLQDLFDPSRNMAKYRNVLNSQNLQPPIIPLFPVIKKDLTFLHEGNDSKVEGLVNFEKLRMIAKEIRHVGRMASVNMDPALMFRTRKKKWRSLGSLSQGSTNAAVLDVAQTGGHKKRVRRSSFLNAKKLYEDAQMARKVKQYLSNLDLEMDEESLQTLSLQCEPATNTLPKNTGDKRSGKSETSPVAPRAGIQQKVQQQQQHKVNQALQVPAVSLYPSRKKVPVKDLPPFGINSPQALKKILSLSEEGSLDRHKKQSEDTVSSASSQLSSPPTSPQSSPRKGGSADHLRPYGSRLTELTSWSSPLGSENSNKNNNSPKISGIGGAYGNKMGKSKSNWISRDLKVGEMNMSRKCKCLSKISIEVIFTKVRWRRMLCREDLDYHLESL

Target ORF information:

RefSeq Version	XM_011574276.1
Organism	Aquila chrysaetos canadensis
Definition	Aquila chrysaetos canadensis Rap guanine nucleotide exchange factor (GEF) 2 (RAPGEF2), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011574276.1

ORF Insert Sequence:

ATGGAAGCCT TGTGTAATCT GAGAGAGCAT CAGCTAAGAC TGATGTGTGA AACTGTTAGA 
TATGAAAGGC ATGAAGCAAA TGAAGTTCTG TACTACCCTG ATGATATTGG TACCTGTTGG 
TATATCCTGC TGTCTGGTTC AGTCTTCATT AAAGAATCTA TGTTTCTTCC AAGGAGCAGT 
TTTGGCAAGC GTTCTGCAGG AAGCTTCAGG CGTGGCTGTG AATGCATTGT TTTAGAGCCA 
TCTGAAATGA TTGTGGTTGA TTACATGGAT GAAAATGAGG AGTATTTTCA GCGTCAAGCT 
TCTCATAGGC AATCTCGAAG AAGATTTAGG AAAATCAACC AAAAAGGGGA GCGACAAACA 
ATTATTGATA CTGTTGACCC TTATCCTGTG GGGAAGCCAC CTCTACCTAG AGGTTACCAT 
ACGGAATGCA CTAAACCTCA GCTTCCTGCA GATTTCACAA AGCTGCACCT TACTGACAGT 
CTCCACCCAC AGGTGACCCA CGTTTCATCT AGTCACTCAG GATGTAGCAT CACTAGTGAT 
TCAGGAAGCA GCAGCCTTTC TGATATCTAC CAGGCTACAG AAAGTGAGGC TGGTGATATG 
GATCTTAGTG GGTTGCCAGA GACAGCAGTG GATTCTGAGG ATGATGATGA TGAAGAAGAC 
ATTGAAAGGG CATCGGATCC TTTGATGAGT AGGGATATTG TTAGAGACTG CTTGGAGAAA 
GACCCAATAG ACAGAACAGA TGATGACATT GAACAACTAC TGGAATTCAT GCATCAATTG 
CCTGCTTTCG CCAACATGAC AATGTCAGTG AGGAGGGAGC TCTGTGCTGT GATGGTGTTT 
GCAGTTGTGG AGAGAGCAGG AACTATTGTA CTGAACGATG GGGAAGAGCT GGATTCCTGG 
TCAGTCATTT TGAATGGCTC TGTGGAGGTG ACGTACCCTG ATGGAAGAAC AGAGATACTC 
TGCATGGGGA ACAGTTTTGG TGTTTCCCCT ACCATGGAAA AGGAATATAT GAAAGGAGTG 
ATGAGAACCA AAGTGGATGA CTGCCAGTTT GTTTGTATAG CCCAGCAAGA TTATTGCCGC 
ATCCTCAATC AAGTAGAAAA GAACATGCAG AAGGTAGAAG AGGAAGGGGA GATTGTGATG 
GTGAAGGAGC ACAGGGAGCT TGATCGCACT GGAACAAGAA AAGGTCACAT TGTCATCAAG 
GGAACAGCTG AAAGGTTAAC AATGCATTTG GTGGAAGAGC ACTCTGTGGT AGACCCAACA 
TTTATAGAGG ACTTCCTGTT GACATATCGG ACCTTTCTTT CCAGCCCGAT GGAAGTGGGC 
AAGAAGTTGT TGGAGTGGTT CAATGACCCC AGCCTCAGGG ATAAGGTTAC ACGGGTAGTA 
TTGTTGTGGG TGAACAATCA CTTCAATGAT TTTGAAGGAG ATCCTGCTAT GACTCGATTT 
TTGGAAGAAT TTGAGAACAA TTTGGAGAGG GAGAAAATGG GTGGACATTT GAGGCTGTTA 
AATATTGCTT GTGCTGCTAA AGCTAAACGA AGATTGATAA CCTTAACAAA GCCATCTCGA 
GAAGCCCCTT TGCCTTTTAT CTTGCTGGGA GGGTCAGAAA AGGGATTTGG AATCTTTGTT 
GACAGTGTAG ATTTTGGTAG CAAAGCCACA GAAGCAGGCT TGAAACGTGG AGACCAGATA 
CTGGAAGTGA ATGGTCAAAA CTTTGAAAAC ATTCAGCTGT CAAAAGCCAT GGAAATCCTT 
AGAAATAACA CTCATCTGTC TATCACTGTG AAAACAAATT TATTTGTTTT TAAAGAACTC 
TTAACACGGT TGTCAGAGGA AAAAAGAAAT GGTGCTCCCC ACCTGCCTAA AATTGGTGAT 
ATTAAAAAGG CGAGTCGTTA TTCCATCCCA GACCTTGCTG TTGATGTGGA GCAGGTAATA 
GGATTAGAAA AAGTAAATAA GAAAAGTAAA GCCAACACAG TTGGGGGAAG AAATAAATTA 
AAGAAGATAC TTGACAAGAC TCGAATCAGT ATTCTGCCTC AGAAACCGTA CAATGACATT 
GGAATTGGCC AGTCTCAGGA TGACAGCATT GTGGGACTGA GGCAAACAAA GCACATTCCT 
CCTGCTTTAC CTGTCAGCGG AACCCTGTCA TCCAGTAATC CCGATCTACT GCAGTCTCAT 
CACCGCATCT TAGACTTCAA TACTACTCCG GACTTACCAG ATCAAGTTCT GAGGGTTTTC 
AAGGCAGATC AGCAAAGTCG CTACATCATG ATCAGTAAGG ACACAACAGC AAAAGAAGTG 
GTCATCCAGG CTATCAGGGA GTTTGCTCTC ACTGCAACCC CTGATGCATA TTCACTATGC 
GAAGTGTCTG TCACACCTGA GGGTGTGATC AAGCAAAGGA GGCTTCCTGA TCAGTTATCC 
AAGCTTGCTG ACAGGATACA GCTGAGTGGC AGGTATTACC TGAAGAACAA CATGGAAACA 
GAAACTCTTT GTTCAGATGA AGATGCTCAA GAGTTACTAA GGGAAAGTCA AATTTCCCTA 
TTACAGCTCA GTACTGTTGA GGTGGCTACC CAACTCTCCA TGAGAAACTT CGAGCTATTC 
CGTAATATTG AACCCACGGA ATACATAGAT GACTTGTTTA AACTCAAATC AAAAACAGGT 
TGCACTAATC TAAAAAAATT TGAAGAGGTG ATAAATCAAG AAACATTCTG GGTGGCTTCT 
GAGATTCTAA GAGAAACCAA CCAGCTGAAA AGGATGAAGA TCATTAAGCA TTTCATTAAG 
ATAGCACTAC ACTGCAGAGA ATGCAAGAAC TTCAACTCAA TGTTTGCCAT CATTAGTGGC 
CTGAATTTGG CACCAGTTGC AAGGCTCCGA ACTACTTGGG AAAAGCTTCC AAGCAAGTAT 
GAAAAACTGT TTCAAGATCT ACAGGACTTG TTTGATCCAT CTAGGAATAT GGCAAAATAT 
CGTAATGTCC TTAACAGCCA AAACCTACAG CCCCCCATTA TACCCCTGTT TCCTGTTATC 
AAAAAAGACC TTACATTCCT TCATGAAGGA AATGATTCAA AAGTGGAGGG CTTGGTCAAT 
TTTGAGAAGC TGAGAATGAT TGCAAAGGAG ATACGCCATG TCGGTCGCAT GGCATCTGTG 
AACATGGATC CTGCTTTAAT GTTTAGAACA AGGAAGAAGA AATGGAGGAG TTTGGGGTCT 
CTCAGCCAGG GCAGTACAAA TGCAGCTGTG CTGGATGTCG CGCAAACGGG GGGTCATAAA 
AAGCGGGTCC GTCGCAGCTC CTTCCTTAAT GCTAAAAAAC TGTATGAAGA TGCTCAGATG 
GCACGGAAAG TGAAGCAGTA TCTCTCAAAC TTAGATCTGG AAATGGATGA AGAGAGCCTC 
CAGACACTGT CTCTGCAGTG TGAGCCAGCC ACCAACACAC TGCCGAAGAA TACAGGAGAC 
AAGCGATCTG GAAAGTCTGA AACGTCACCG GTGGCTCCCC GGGCAGGCAT CCAGCAGAAA 
GTGCAGCAGC AGCAGCAACA TAAAGTAAAC CAAGCATTGC AGGTCCCTGC CGTATCTCTT 
TATCCCTCTC GCAAGAAAGT GCCGGTCAAA GACCTCCCAC CATTTGGCAT TAACTCCCCA 
CAAGCTTTAA AGAAAATTCT TTCCTTATCT GAAGAAGGAA GTTTGGATCG TCACAAGAAA 
CAATCTGAAG ACACGGTTTC AAGTGCGTCT TCGCAGCTGT CTTCTCCTCC CACTTCACCA 
CAGAGCTCTC CAAGGAAAGG TGGCAGTGCT GATCATTTGA GGCCTTATGG ATCCAGACTA 
ACAGAGCTAA CCAGTTGGTC CTCTCCTCTT GGAAGTGAGA ACAGTAACAA GAATAACAAT 
TCACCAAAGA TCTCTGGGAT AGGTGGGGCT TATGGAAACA AAATGGGAAA AAGTAAGTCC 
AATTGGATAA GCAGAGATCT GAAAGTGGGA GAGATGAATA TGTCAAGAAA GTGCAAATGC 
CTATCCAAAA TCTCTATTGA AGTGATATTT ACTAAGGTCA GATGGAGGAG AATGTTATGT 
AGAGAAGATT TAGATTATCA CCTCGAGTCC TTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF2 ( XM_011574270.1 ) cDNA ORF clone, Aquila chrysaetos canadensis -> XP_011572572.1 Aquila chrysaetos canadensis Rap guanine nucleotide exchange factor (GEF) 2 (RAPGEF2), transcript variant X1, mRNA.

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CloneID	OAq14194
Clone ID Related Accession (Same CDS sequence)	XM_011574270.1
Accession Version	XM_011574270.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4857bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-12
Organism	Aquila chrysaetos canadensis
Product	rap guanine nucleotide exchange factor 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011950881.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Aquila chrysaetos canadensis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGCCT TGTGTAATCT GAGAGAGCAT CAGCTAAGAC TGATGTGTGA AACTGTTAGA 
TATGAAAGGC ATGAAGCAAA TGAAGTTCTG TACTACCCTG ATGATATTGG TACCTGTTGG 
TATATCCTGC TGTCTGGTTC AGTCTTCATT AAAGAATCTA TGTTTCTTCC AAGGAGCAGT 
TTTGGCAAGC GTTCTGCAGG AAGCTTCAGG CGTGGCTGTG AATGCATTGT TTTAGAGCCA 
TCTGAAATGA TTGTGGTTGA TTACATGGAT GAAAATGAGG AGTATTTTCA GCGTCAAGCT 
TCTCATAGGC AATCTCGAAG AAGATTTAGG AAAATCAACC AAAAAGGGGA GCGACAAACA 
ATTATTGATA CTGTTGACCC TTATCCTGTG GGGAAGCCAC CTCTACCTAG AGGTTACCAT 
ACGGAATGCA CTAAACCTCA GCTTCCTGCA GATTTCACAA AGCTGCACCT TACTGACAGT 
CTCCACCCAC AGGTGACCCA CGTTTCATCT AGTCACTCAG GATGTAGCAT CACTAGTGAT 
TCAGGAAGCA GCAGCCTTTC TGATATCTAC CAGGCTACAG AAAGTGAGGC TGGTGATATG 
GATCTTAGTG GGTTGCCAGA GACAGCAGTG GATTCTGAGG ATGATGATGA TGAAGAAGAC 
ATTGAAAGGG CATCGGATCC TTTGATGAGT AGGGATATTG TTAGAGACTG CTTGGAGAAA 
GACCCAATAG ACAGAACAGA TGATGACATT GAACAACTAC TGGAATTCAT GCATCAATTG 
CCTGCTTTCG CCAACATGAC AATGTCAGTG AGGAGGGAGC TCTGTGCTGT GATGGTGTTT 
GCAGTTGTGG AGAGAGCAGG AACTATTGTA CTGAACGATG GGGAAGAGCT GGATTCCTGG 
TCAGTCATTT TGAATGGCTC TGTGGAGGTG ACGTACCCTG ATGGAAGAAC AGAGATACTC 
TGCATGGGGA ACAGTTTTGG TGTTTCCCCT ACCATGGAAA AGGAATATAT GAAAGGAGTG 
ATGAGAACCA AAGTGGATGA CTGCCAGTTT GTTTGTATAG CCCAGCAAGA TTATTGCCGC 
ATCCTCAATC AAGTAGAAAA GAACATGCAG AAGGTAGAAG AGGAAGGGGA GATTGTGATG 
GTGAAGGAGC ACAGGGAGCT TGATCGCACT GGAACAAGAA AAGGTCACAT TGTCATCAAG 
GGAACAGCTG AAAGGTTAAC AATGCATTTG GTGGAAGAGC ACTCTGTGGT AGACCCAACA 
TTTATAGAGG ACTTCCTGTT GACATATCGG ACCTTTCTTT CCAGCCCGAT GGAAGTGGGC 
AAGAAGTTGT TGGAGTGGTT CAATGACCCC AGCCTCAGGG ATAAGGTTAC ACGGGTAGTA 
TTGTTGTGGG TGAACAATCA CTTCAATGAT TTTGAAGGAG ATCCTGCTAT GACTCGATTT 
TTGGAAGAAT TTGAGAACAA TTTGGAGAGG GAGAAAATGG GTGGACATTT GAGGCTGTTA 
AATATTGCTT GTGCTGCTAA AGCTAAACGA AGATTGATAA CCTTAACAAA GCCATCTCGA 
GAAGCCCCTT TGCCTTTTAT CTTGCTGGGA GGGTCAGAAA AGGGATTTGG AATCTTTGTT 
GACAGTGTAG ATTTTGGTAG CAAAGCCACA GAAGCAGGCT TGAAACGTGG AGACCAGATA 
CTGGAAGTGA ATGGTCAAAA CTTTGAAAAC ATTCAGCTGT CAAAAGCCAT GGAAATCCTT 
AGAAATAACA CTCATCTGTC TATCACTGTG AAAACAAATT TATTTGTTTT TAAAGAACTC 
TTAACACGGT TGTCAGAGGA AAAAAGAAAT GGTGCTCCCC ACCTGCCTAA AATTGGTGAT 
ATTAAAAAGG CGAGTCGTTA TTCCATCCCA GACCTTGCTG TTGATGTGGA GCAGGTAATA 
GGATTAGAAA AAGTAAATAA GAAAAGTAAA GCCAACACAG TTGGGGGAAG AAATAAATTA 
AAGAAGATAC TTGACAAGAC TCGAATCAGT ATTCTGCCTC AGAAACCGTA CAATGACATT 
GGAATTGGCC AGTCTCAGGA TGACAGCATT GTGGGACTGA GGCAAACAAA GCACATTCCT 
CCTGCTTTAC CTGTCAGCGG AACCCTGTCA TCCAGTAATC CCGATCTACT GCAGTCTCAT 
CACCGCATCT TAGACTTCAA TACTACTCCG GACTTACCAG ATCAAGTTCT GAGGGTTTTC 
AAGGCAGATC AGCAAAGTCG CTACATCATG ATCAGTAAGG ACACAACAGC AAAAGAAGTG 
GTCATCCAGG CTATCAGGGA GTTTGCTCTC ACTGCAACCC CTGATGCATA TTCACTATGC 
GAAGTGTCTG TCACACCTGA GGGTGTGATC AAGCAAAGGA GGCTTCCTGA TCAGTTATCC 
AAGCTTGCTG ACAGGATACA GCTGAGTGGC AGGTATTACC TGAAGAACAA CATGGAAACA 
GAAACTCTTT GTTCAGATGA AGATGCTCAA GAGTTACTAA GGGAAAGTCA AATTTCCCTA 
TTACAGCTCA GTACTGTTGA GGTGGCTACC CAACTCTCCA TGAGAAACTT CGAGCTATTC 
CGTAATATTG AACCCACGGA ATACATAGAT GACTTGTTTA AACTCAAATC AAAAACAGGT 
TGCACTAATC TAAAAAAATT TGAAGAGGTG ATAAATCAAG AAACATTCTG GGTGGCTTCT 
GAGATTCTAA GAGAAACCAA CCAGCTGAAA AGGATGAAGA TCATTAAGCA TTTCATTAAG 
ATAGCACTAC ACTGCAGAGA ATGCAAGAAC TTCAACTCAA TGTTTGCCAT CATTAGTGGC 
CTGAATTTGG CACCAGTTGC AAGGCTCCGA ACTACTTGGG AAAAGCTTCC AAGCAAGTAT 
GAAAAACTGT TTCAAGATCT ACAGGACTTG TTTGATCCAT CTAGGAATAT GGCAAAATAT 
CGTAATGTCC TTAACAGCCA AAACCTACAG CCCCCCATTA TACCCCTGTT TCCTGTTATC 
AAAAAAGACC TTACATTCCT TCATGAAGGA AATGATTCAA AAGTGGAGGG CTTGGTCAAT 
TTTGAGAAGC TGAGAATGAT TGCAAAGGAG ATACGCCATG TCGGTCGCAT GGCATCTGTG 
AACATGGATC CTGCTTTAAT GTTTAGAACA AGGAAGAAGA AATGGAGGAG TTTGGGGTCT 
CTCAGCCAGG GCAGTACAAA TGCAGCTGTG CTGGATGTCG CGCAAACGGG GGGTCATAAA 
AAGCGGGTCC GTCGCAGCTC CTTCCTTAAT GCTAAAAAAC TGTATGAAGA TGCTCAGATG 
GCACGGAAAG TGAAGCAGTA TCTCTCAAAC TTAGATCTGG AAATGGATGA AGAGAGCCTC 
CAGACACTGT CTCTGCAGTG TGAGCCAGCC ACCAACACAC TGCCGAAGAA TACAGGAGAC 
AAGCGATCTG GAAAGTCTGA AACGTCACCG GTGGCTCCCC GGGCAGGCAT CCAGCAGAAA 
GTGCAGCAGC AGCAGCAACA TAAAGTAAAC CAAGCATTGC AGGTCCCTGC CGTATCTCTT 
TATCCCTCTC GCAAGAAAGT GCCGGTCAAA GACCTCCCAC CATTTGGCAT TAACTCCCCA 
CAAGCTTTAA AGAAAATTCT TTCCTTATCT GAAGAAGGAA GTTTGGATCG TCACAAGAAA 
CAATCTGAAG ACACGGTTTC AAGTGCGTCT TCGCAGCTGT CTTCTCCTCC CACTTCACCA 
CAGAGCTCTC CAAGGAAAGG CTATACTTTG GCTCCTAGCG GCACAGTGGA TAACTTTTCA 
GATTCTGGTC ACAGCGAAAT TTCTTCCAGA TCTAGTATTG TCAGCAATTC CTCTTTTGAC 
TCTATGCCAG TCTCCCTGCA TGATGAGAGG AGACAGAGAC ATTCTGTCAG CATCGTGGAG 
ACAAATCTTG GTGTGGGAAG GATTGATAGA AGAATCATGA TTGAACCAGA TCAATACAGC 
TTAGGCTCAT ATGCACCACT GTCAGAGACC AGAGGCCTGT ATGCTGGAGC AACTGTGCTT 
TCTTCTCCTA GTACAGAAGA ACTGTCACAG GATCAGGGGG ACAGAGCTTC GCTCGATGCA 
GCTGACAGTG GCCGTGGTAG CTGGACTTCA TGTTCGAGCG GTTCTCATGA CAACATACAA 
ACAATACAGC ACCAGAGAAG CTGGGAGACT CTGCCTTTTG GACATGCTCA TTTCGATAGT 
TCAGGCGATG GGGCAGGACT GTGGGCCTCG GGCAGTCATA TGGACCAGCT GATGTTCCCT 
GATCATGGCA CAAAGTATGG CAGGCAAAGT CAAGGTAGGG AGGGCCTTGA TCAAGCACAG 
TCCAGAGCAA GCTGGGCATC CTCGACAGGA TACTGGGGAG AGGACTCGGA AGGTGATACA 
GGTACCATAA AGCGGAGGGG TGGGAAGGAT GTTTCAATTG AAGCTGAAAC CAGTAGCATA 
ACATCTGTAC CAGCAGAGGA GTCAAAGCCA GCTCCCATGC CCACTCATAT AGCAGTATCA 
TCAAGTAGTA CAAAGGGGCT TATTGCACGA AAAGAGGGTC GATACCGGGA ACCACCTCCA 
ACTCCTCCTG GTTACGTAGG AATACCTATT GCTGAGTTTG CAGAAGGTGG TTCCCATCCA 
ACCAGAAAGC CTCCAGATTA CAACATAGCA CTTCAGAGGT CTAGAATGGT GGCGCGGACA 
TGTGAGACTC ATGGGACATC AACTCCACAG CAGCAGTCAC ACGGCCATTC AACTAGCAGG 
CCTGTGAACA AACCCCAGTG GCATAAACCA AATGAGTCAG ATCCACGTCT TGCTCACTAT 
CCATCTCAAG GGTTTTCCAC AGAGGAAGAT GAAGATGAAC AAGTCTCTGC TGTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011572572.1
CDS	30..4886
Translation	MEALCNLREHQLRLMCETVRYERHEANEVLYYPDDIGTCWYILLSGSVFIKESMFLPRSSFGKRSAGSFRRGCECIVLEPSEMIVVDYMDENEEYFQRQASHRQSRRRFRKINQKGERQTIIDTVDPYPVGKPPLPRGYHTECTKPQLPADFTKLHLTDSLHPQVTHVSSSHSGCSITSDSGSSSLSDIYQATESEAGDMDLSGLPETAVDSEDDDDEEDIERASDPLMSRDIVRDCLEKDPIDRTDDDIEQLLEFMHQLPAFANMTMSVRRELCAVMVFAVVERAGTIVLNDGEELDSWSVILNGSVEVTYPDGRTEILCMGNSFGVSPTMEKEYMKGVMRTKVDDCQFVCIAQQDYCRILNQVEKNMQKVEEEGEIVMVKEHRELDRTGTRKGHIVIKGTAERLTMHLVEEHSVVDPTFIEDFLLTYRTFLSSPMEVGKKLLEWFNDPSLRDKVTRVVLLWVNNHFNDFEGDPAMTRFLEEFENNLEREKMGGHLRLLNIACAAKAKRRLITLTKPSREAPLPFILLGGSEKGFGIFVDSVDFGSKATEAGLKRGDQILEVNGQNFENIQLSKAMEILRNNTHLSITVKTNLFVFKELLTRLSEEKRNGAPHLPKIGDIKKASRYSIPDLAVDVEQVIGLEKVNKKSKANTVGGRNKLKKILDKTRISILPQKPYNDIGIGQSQDDSIVGLRQTKHIPPALPVSGTLSSSNPDLLQSHHRILDFNTTPDLPDQVLRVFKADQQSRYIMISKDTTAKEVVIQAIREFALTATPDAYSLCEVSVTPEGVIKQRRLPDQLSKLADRIQLSGRYYLKNNMETETLCSDEDAQELLRESQISLLQLSTVEVATQLSMRNFELFRNIEPTEYIDDLFKLKSKTGCTNLKKFEEVINQETFWVASEILRETNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLAPVARLRTTWEKLPSKYEKLFQDLQDLFDPSRNMAKYRNVLNSQNLQPPIIPLFPVIKKDLTFLHEGNDSKVEGLVNFEKLRMIAKEIRHVGRMASVNMDPALMFRTRKKKWRSLGSLSQGSTNAAVLDVAQTGGHKKRVRRSSFLNAKKLYEDAQMARKVKQYLSNLDLEMDEESLQTLSLQCEPATNTLPKNTGDKRSGKSETSPVAPRAGIQQKVQQQQQHKVNQALQVPAVSLYPSRKKVPVKDLPPFGINSPQALKKILSLSEEGSLDRHKKQSEDTVSSASSQLSSPPTSPQSSPRKGYTLAPSGTVDNFSDSGHSEISSRSSIVSNSSFDSMPVSLHDERRQRHSVSIVETNLGVGRIDRRIMIEPDQYSLGSYAPLSETRGLYAGATVLSSPSTEELSQDQGDRASLDAADSGRGSWTSCSSGSHDNIQTIQHQRSWETLPFGHAHFDSSGDGAGLWASGSHMDQLMFPDHGTKYGRQSQGREGLDQAQSRASWASSTGYWGEDSEGDTGTIKRRGGKDVSIEAETSSITSVPAEESKPAPMPTHIAVSSSSTKGLIARKEGRYREPPPTPPGYVGIPIAEFAEGGSHPTRKPPDYNIALQRSRMVARTCETHGTSTPQQQSHGHSTSRPVNKPQWHKPNESDPRLAHYPSQGFSTEEDEDEQVSAV

Target ORF information:

RefSeq Version	XM_011574270.1
Organism	Aquila chrysaetos canadensis
Definition	Aquila chrysaetos canadensis Rap guanine nucleotide exchange factor (GEF) 2 (RAPGEF2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011574270.1

ORF Insert Sequence:

ATGGAAGCCT TGTGTAATCT GAGAGAGCAT CAGCTAAGAC TGATGTGTGA AACTGTTAGA 
TATGAAAGGC ATGAAGCAAA TGAAGTTCTG TACTACCCTG ATGATATTGG TACCTGTTGG 
TATATCCTGC TGTCTGGTTC AGTCTTCATT AAAGAATCTA TGTTTCTTCC AAGGAGCAGT 
TTTGGCAAGC GTTCTGCAGG AAGCTTCAGG CGTGGCTGTG AATGCATTGT TTTAGAGCCA 
TCTGAAATGA TTGTGGTTGA TTACATGGAT GAAAATGAGG AGTATTTTCA GCGTCAAGCT 
TCTCATAGGC AATCTCGAAG AAGATTTAGG AAAATCAACC AAAAAGGGGA GCGACAAACA 
ATTATTGATA CTGTTGACCC TTATCCTGTG GGGAAGCCAC CTCTACCTAG AGGTTACCAT 
ACGGAATGCA CTAAACCTCA GCTTCCTGCA GATTTCACAA AGCTGCACCT TACTGACAGT 
CTCCACCCAC AGGTGACCCA CGTTTCATCT AGTCACTCAG GATGTAGCAT CACTAGTGAT 
TCAGGAAGCA GCAGCCTTTC TGATATCTAC CAGGCTACAG AAAGTGAGGC TGGTGATATG 
GATCTTAGTG GGTTGCCAGA GACAGCAGTG GATTCTGAGG ATGATGATGA TGAAGAAGAC 
ATTGAAAGGG CATCGGATCC TTTGATGAGT AGGGATATTG TTAGAGACTG CTTGGAGAAA 
GACCCAATAG ACAGAACAGA TGATGACATT GAACAACTAC TGGAATTCAT GCATCAATTG 
CCTGCTTTCG CCAACATGAC AATGTCAGTG AGGAGGGAGC TCTGTGCTGT GATGGTGTTT 
GCAGTTGTGG AGAGAGCAGG AACTATTGTA CTGAACGATG GGGAAGAGCT GGATTCCTGG 
TCAGTCATTT TGAATGGCTC TGTGGAGGTG ACGTACCCTG ATGGAAGAAC AGAGATACTC 
TGCATGGGGA ACAGTTTTGG TGTTTCCCCT ACCATGGAAA AGGAATATAT GAAAGGAGTG 
ATGAGAACCA AAGTGGATGA CTGCCAGTTT GTTTGTATAG CCCAGCAAGA TTATTGCCGC 
ATCCTCAATC AAGTAGAAAA GAACATGCAG AAGGTAGAAG AGGAAGGGGA GATTGTGATG 
GTGAAGGAGC ACAGGGAGCT TGATCGCACT GGAACAAGAA AAGGTCACAT TGTCATCAAG 
GGAACAGCTG AAAGGTTAAC AATGCATTTG GTGGAAGAGC ACTCTGTGGT AGACCCAACA 
TTTATAGAGG ACTTCCTGTT GACATATCGG ACCTTTCTTT CCAGCCCGAT GGAAGTGGGC 
AAGAAGTTGT TGGAGTGGTT CAATGACCCC AGCCTCAGGG ATAAGGTTAC ACGGGTAGTA 
TTGTTGTGGG TGAACAATCA CTTCAATGAT TTTGAAGGAG ATCCTGCTAT GACTCGATTT 
TTGGAAGAAT TTGAGAACAA TTTGGAGAGG GAGAAAATGG GTGGACATTT GAGGCTGTTA 
AATATTGCTT GTGCTGCTAA AGCTAAACGA AGATTGATAA CCTTAACAAA GCCATCTCGA 
GAAGCCCCTT TGCCTTTTAT CTTGCTGGGA GGGTCAGAAA AGGGATTTGG AATCTTTGTT 
GACAGTGTAG ATTTTGGTAG CAAAGCCACA GAAGCAGGCT TGAAACGTGG AGACCAGATA 
CTGGAAGTGA ATGGTCAAAA CTTTGAAAAC ATTCAGCTGT CAAAAGCCAT GGAAATCCTT 
AGAAATAACA CTCATCTGTC TATCACTGTG AAAACAAATT TATTTGTTTT TAAAGAACTC 
TTAACACGGT TGTCAGAGGA AAAAAGAAAT GGTGCTCCCC ACCTGCCTAA AATTGGTGAT 
ATTAAAAAGG CGAGTCGTTA TTCCATCCCA GACCTTGCTG TTGATGTGGA GCAGGTAATA 
GGATTAGAAA AAGTAAATAA GAAAAGTAAA GCCAACACAG TTGGGGGAAG AAATAAATTA 
AAGAAGATAC TTGACAAGAC TCGAATCAGT ATTCTGCCTC AGAAACCGTA CAATGACATT 
GGAATTGGCC AGTCTCAGGA TGACAGCATT GTGGGACTGA GGCAAACAAA GCACATTCCT 
CCTGCTTTAC CTGTCAGCGG AACCCTGTCA TCCAGTAATC CCGATCTACT GCAGTCTCAT 
CACCGCATCT TAGACTTCAA TACTACTCCG GACTTACCAG ATCAAGTTCT GAGGGTTTTC 
AAGGCAGATC AGCAAAGTCG CTACATCATG ATCAGTAAGG ACACAACAGC AAAAGAAGTG 
GTCATCCAGG CTATCAGGGA GTTTGCTCTC ACTGCAACCC CTGATGCATA TTCACTATGC 
GAAGTGTCTG TCACACCTGA GGGTGTGATC AAGCAAAGGA GGCTTCCTGA TCAGTTATCC 
AAGCTTGCTG ACAGGATACA GCTGAGTGGC AGGTATTACC TGAAGAACAA CATGGAAACA 
GAAACTCTTT GTTCAGATGA AGATGCTCAA GAGTTACTAA GGGAAAGTCA AATTTCCCTA 
TTACAGCTCA GTACTGTTGA GGTGGCTACC CAACTCTCCA TGAGAAACTT CGAGCTATTC 
CGTAATATTG AACCCACGGA ATACATAGAT GACTTGTTTA AACTCAAATC AAAAACAGGT 
TGCACTAATC TAAAAAAATT TGAAGAGGTG ATAAATCAAG AAACATTCTG GGTGGCTTCT 
GAGATTCTAA GAGAAACCAA CCAGCTGAAA AGGATGAAGA TCATTAAGCA TTTCATTAAG 
ATAGCACTAC ACTGCAGAGA ATGCAAGAAC TTCAACTCAA TGTTTGCCAT CATTAGTGGC 
CTGAATTTGG CACCAGTTGC AAGGCTCCGA ACTACTTGGG AAAAGCTTCC AAGCAAGTAT 
GAAAAACTGT TTCAAGATCT ACAGGACTTG TTTGATCCAT CTAGGAATAT GGCAAAATAT 
CGTAATGTCC TTAACAGCCA AAACCTACAG CCCCCCATTA TACCCCTGTT TCCTGTTATC 
AAAAAAGACC TTACATTCCT TCATGAAGGA AATGATTCAA AAGTGGAGGG CTTGGTCAAT 
TTTGAGAAGC TGAGAATGAT TGCAAAGGAG ATACGCCATG TCGGTCGCAT GGCATCTGTG 
AACATGGATC CTGCTTTAAT GTTTAGAACA AGGAAGAAGA AATGGAGGAG TTTGGGGTCT 
CTCAGCCAGG GCAGTACAAA TGCAGCTGTG CTGGATGTCG CGCAAACGGG GGGTCATAAA 
AAGCGGGTCC GTCGCAGCTC CTTCCTTAAT GCTAAAAAAC TGTATGAAGA TGCTCAGATG 
GCACGGAAAG TGAAGCAGTA TCTCTCAAAC TTAGATCTGG AAATGGATGA AGAGAGCCTC 
CAGACACTGT CTCTGCAGTG TGAGCCAGCC ACCAACACAC TGCCGAAGAA TACAGGAGAC 
AAGCGATCTG GAAAGTCTGA AACGTCACCG GTGGCTCCCC GGGCAGGCAT CCAGCAGAAA 
GTGCAGCAGC AGCAGCAACA TAAAGTAAAC CAAGCATTGC AGGTCCCTGC CGTATCTCTT 
TATCCCTCTC GCAAGAAAGT GCCGGTCAAA GACCTCCCAC CATTTGGCAT TAACTCCCCA 
CAAGCTTTAA AGAAAATTCT TTCCTTATCT GAAGAAGGAA GTTTGGATCG TCACAAGAAA 
CAATCTGAAG ACACGGTTTC AAGTGCGTCT TCGCAGCTGT CTTCTCCTCC CACTTCACCA 
CAGAGCTCTC CAAGGAAAGG CTATACTTTG GCTCCTAGCG GCACAGTGGA TAACTTTTCA 
GATTCTGGTC ACAGCGAAAT TTCTTCCAGA TCTAGTATTG TCAGCAATTC CTCTTTTGAC 
TCTATGCCAG TCTCCCTGCA TGATGAGAGG AGACAGAGAC ATTCTGTCAG CATCGTGGAG 
ACAAATCTTG GTGTGGGAAG GATTGATAGA AGAATCATGA TTGAACCAGA TCAATACAGC 
TTAGGCTCAT ATGCACCACT GTCAGAGACC AGAGGCCTGT ATGCTGGAGC AACTGTGCTT 
TCTTCTCCTA GTACAGAAGA ACTGTCACAG GATCAGGGGG ACAGAGCTTC GCTCGATGCA 
GCTGACAGTG GCCGTGGTAG CTGGACTTCA TGTTCGAGCG GTTCTCATGA CAACATACAA 
ACAATACAGC ACCAGAGAAG CTGGGAGACT CTGCCTTTTG GACATGCTCA TTTCGATAGT 
TCAGGCGATG GGGCAGGACT GTGGGCCTCG GGCAGTCATA TGGACCAGCT GATGTTCCCT 
GATCATGGCA CAAAGTATGG CAGGCAAAGT CAAGGTAGGG AGGGCCTTGA TCAAGCACAG 
TCCAGAGCAA GCTGGGCATC CTCGACAGGA TACTGGGGAG AGGACTCGGA AGGTGATACA 
GGTACCATAA AGCGGAGGGG TGGGAAGGAT GTTTCAATTG AAGCTGAAAC CAGTAGCATA 
ACATCTGTAC CAGCAGAGGA GTCAAAGCCA GCTCCCATGC CCACTCATAT AGCAGTATCA 
TCAAGTAGTA CAAAGGGGCT TATTGCACGA AAAGAGGGTC GATACCGGGA ACCACCTCCA 
ACTCCTCCTG GTTACGTAGG AATACCTATT GCTGAGTTTG CAGAAGGTGG TTCCCATCCA 
ACCAGAAAGC CTCCAGATTA CAACATAGCA CTTCAGAGGT CTAGAATGGT GGCGCGGACA 
TGTGAGACTC ATGGGACATC AACTCCACAG CAGCAGTCAC ACGGCCATTC AACTAGCAGG 
CCTGTGAACA AACCCCAGTG GCATAAACCA AATGAGTCAG ATCCACGTCT TGCTCACTAT 
CCATCTCAAG GGTTTTCCAC AGAGGAAGAT GAAGATGAAC AAGTCTCTGC TGTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF2 ( XM_011574271.1 ) cDNA ORF clone, Aquila chrysaetos canadensis -> XP_011572573.1 Aquila chrysaetos canadensis Rap guanine nucleotide exchange factor (GEF) 2 (RAPGEF2), transcript variant X2, mRNA.

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CloneID	OAq14195
Clone ID Related Accession (Same CDS sequence)	XM_011574271.1
Accession Version	XM_011574271.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4839bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-12
Organism	Aquila chrysaetos canadensis
Product	rap guanine nucleotide exchange factor 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011950881.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Aquila chrysaetos canadensis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGCCT TGTGTAATCT GAGAGAGCAT CAGCTAAGAC TGATGTGTGA AACTGTTAGA 
TATGAAAGGC ATGAAGCAAA TGAAGTTCTG TACTACCCTG ATGATATTGG TACCTGTTGG 
TATATCCTGC TGTCTGGTTC AGTCTTCATT AAAGAATCTA TGTTTCTTCC AAGGAGCAGT 
TTTGGCAAGC GTTCTGCAGG AAGCTTCAGG CGTGGCTGTG AATGCATTGT TTTAGAGCCA 
TCTGAAATGA TTGTGGTTGA TTACATGGAT GAAAATGAGG AGTATTTTCA GCGTCAAGCT 
TCTCATAGGC AATCTCGAAG AAGATTTAGG AAAATCAACC AAAAAGGGGA GCGACAAACA 
ATTATTGATA CTGTTGACCC TTATCCTGTG GGGAAGCCAC CTCTACCTAG AGGTTACCAT 
ACGCTTCCTG CAGATTTCAC AAAGCTGCAC CTTACTGACA GTCTCCACCC ACAGGTGACC 
CACGTTTCAT CTAGTCACTC AGGATGTAGC ATCACTAGTG ATTCAGGAAG CAGCAGCCTT 
TCTGATATCT ACCAGGCTAC AGAAAGTGAG GCTGGTGATA TGGATCTTAG TGGGTTGCCA 
GAGACAGCAG TGGATTCTGA GGATGATGAT GATGAAGAAG ACATTGAAAG GGCATCGGAT 
CCTTTGATGA GTAGGGATAT TGTTAGAGAC TGCTTGGAGA AAGACCCAAT AGACAGAACA 
GATGATGACA TTGAACAACT ACTGGAATTC ATGCATCAAT TGCCTGCTTT CGCCAACATG 
ACAATGTCAG TGAGGAGGGA GCTCTGTGCT GTGATGGTGT TTGCAGTTGT GGAGAGAGCA 
GGAACTATTG TACTGAACGA TGGGGAAGAG CTGGATTCCT GGTCAGTCAT TTTGAATGGC 
TCTGTGGAGG TGACGTACCC TGATGGAAGA ACAGAGATAC TCTGCATGGG GAACAGTTTT 
GGTGTTTCCC CTACCATGGA AAAGGAATAT ATGAAAGGAG TGATGAGAAC CAAAGTGGAT 
GACTGCCAGT TTGTTTGTAT AGCCCAGCAA GATTATTGCC GCATCCTCAA TCAAGTAGAA 
AAGAACATGC AGAAGGTAGA AGAGGAAGGG GAGATTGTGA TGGTGAAGGA GCACAGGGAG 
CTTGATCGCA CTGGAACAAG AAAAGGTCAC ATTGTCATCA AGGGAACAGC TGAAAGGTTA 
ACAATGCATT TGGTGGAAGA GCACTCTGTG GTAGACCCAA CATTTATAGA GGACTTCCTG 
TTGACATATC GGACCTTTCT TTCCAGCCCG ATGGAAGTGG GCAAGAAGTT GTTGGAGTGG 
TTCAATGACC CCAGCCTCAG GGATAAGGTT ACACGGGTAG TATTGTTGTG GGTGAACAAT 
CACTTCAATG ATTTTGAAGG AGATCCTGCT ATGACTCGAT TTTTGGAAGA ATTTGAGAAC 
AATTTGGAGA GGGAGAAAAT GGGTGGACAT TTGAGGCTGT TAAATATTGC TTGTGCTGCT 
AAAGCTAAAC GAAGATTGAT AACCTTAACA AAGCCATCTC GAGAAGCCCC TTTGCCTTTT 
ATCTTGCTGG GAGGGTCAGA AAAGGGATTT GGAATCTTTG TTGACAGTGT AGATTTTGGT 
AGCAAAGCCA CAGAAGCAGG CTTGAAACGT GGAGACCAGA TACTGGAAGT GAATGGTCAA 
AACTTTGAAA ACATTCAGCT GTCAAAAGCC ATGGAAATCC TTAGAAATAA CACTCATCTG 
TCTATCACTG TGAAAACAAA TTTATTTGTT TTTAAAGAAC TCTTAACACG GTTGTCAGAG 
GAAAAAAGAA ATGGTGCTCC CCACCTGCCT AAAATTGGTG ATATTAAAAA GGCGAGTCGT 
TATTCCATCC CAGACCTTGC TGTTGATGTG GAGCAGGTAA TAGGATTAGA AAAAGTAAAT 
AAGAAAAGTA AAGCCAACAC AGTTGGGGGA AGAAATAAAT TAAAGAAGAT ACTTGACAAG 
ACTCGAATCA GTATTCTGCC TCAGAAACCG TACAATGACA TTGGAATTGG CCAGTCTCAG 
GATGACAGCA TTGTGGGACT GAGGCAAACA AAGCACATTC CTCCTGCTTT ACCTGTCAGC 
GGAACCCTGT CATCCAGTAA TCCCGATCTA CTGCAGTCTC ATCACCGCAT CTTAGACTTC 
AATACTACTC CGGACTTACC AGATCAAGTT CTGAGGGTTT TCAAGGCAGA TCAGCAAAGT 
CGCTACATCA TGATCAGTAA GGACACAACA GCAAAAGAAG TGGTCATCCA GGCTATCAGG 
GAGTTTGCTC TCACTGCAAC CCCTGATGCA TATTCACTAT GCGAAGTGTC TGTCACACCT 
GAGGGTGTGA TCAAGCAAAG GAGGCTTCCT GATCAGTTAT CCAAGCTTGC TGACAGGATA 
CAGCTGAGTG GCAGGTATTA CCTGAAGAAC AACATGGAAA CAGAAACTCT TTGTTCAGAT 
GAAGATGCTC AAGAGTTACT AAGGGAAAGT CAAATTTCCC TATTACAGCT CAGTACTGTT 
GAGGTGGCTA CCCAACTCTC CATGAGAAAC TTCGAGCTAT TCCGTAATAT TGAACCCACG 
GAATACATAG ATGACTTGTT TAAACTCAAA TCAAAAACAG GTTGCACTAA TCTAAAAAAA 
TTTGAAGAGG TGATAAATCA AGAAACATTC TGGGTGGCTT CTGAGATTCT AAGAGAAACC 
AACCAGCTGA AAAGGATGAA GATCATTAAG CATTTCATTA AGATAGCACT ACACTGCAGA 
GAATGCAAGA ACTTCAACTC AATGTTTGCC ATCATTAGTG GCCTGAATTT GGCACCAGTT 
GCAAGGCTCC GAACTACTTG GGAAAAGCTT CCAAGCAAGT ATGAAAAACT GTTTCAAGAT 
CTACAGGACT TGTTTGATCC ATCTAGGAAT ATGGCAAAAT ATCGTAATGT CCTTAACAGC 
CAAAACCTAC AGCCCCCCAT TATACCCCTG TTTCCTGTTA TCAAAAAAGA CCTTACATTC 
CTTCATGAAG GAAATGATTC AAAAGTGGAG GGCTTGGTCA ATTTTGAGAA GCTGAGAATG 
ATTGCAAAGG AGATACGCCA TGTCGGTCGC ATGGCATCTG TGAACATGGA TCCTGCTTTA 
ATGTTTAGAA CAAGGAAGAA GAAATGGAGG AGTTTGGGGT CTCTCAGCCA GGGCAGTACA 
AATGCAGCTG TGCTGGATGT CGCGCAAACG GGGGGTCATA AAAAGCGGGT CCGTCGCAGC 
TCCTTCCTTA ATGCTAAAAA ACTGTATGAA GATGCTCAGA TGGCACGGAA AGTGAAGCAG 
TATCTCTCAA ACTTAGATCT GGAAATGGAT GAAGAGAGCC TCCAGACACT GTCTCTGCAG 
TGTGAGCCAG CCACCAACAC ACTGCCGAAG AATACAGGAG ACAAGCGATC TGGAAAGTCT 
GAAACGTCAC CGGTGGCTCC CCGGGCAGGC ATCCAGCAGA AAGTGCAGCA GCAGCAGCAA 
CATAAAGTAA ACCAAGCATT GCAGGTCCCT GCCGTATCTC TTTATCCCTC TCGCAAGAAA 
GTGCCGGTCA AAGACCTCCC ACCATTTGGC ATTAACTCCC CACAAGCTTT AAAGAAAATT 
CTTTCCTTAT CTGAAGAAGG AAGTTTGGAT CGTCACAAGA AACAATCTGA AGACACGGTT 
TCAAGTGCGT CTTCGCAGCT GTCTTCTCCT CCCACTTCAC CACAGAGCTC TCCAAGGAAA 
GGCTATACTT TGGCTCCTAG CGGCACAGTG GATAACTTTT CAGATTCTGG TCACAGCGAA 
ATTTCTTCCA GATCTAGTAT TGTCAGCAAT TCCTCTTTTG ACTCTATGCC AGTCTCCCTG 
CATGATGAGA GGAGACAGAG ACATTCTGTC AGCATCGTGG AGACAAATCT TGGTGTGGGA 
AGGATTGATA GAAGAATCAT GATTGAACCA GATCAATACA GCTTAGGCTC ATATGCACCA 
CTGTCAGAGA CCAGAGGCCT GTATGCTGGA GCAACTGTGC TTTCTTCTCC TAGTACAGAA 
GAACTGTCAC AGGATCAGGG GGACAGAGCT TCGCTCGATG CAGCTGACAG TGGCCGTGGT 
AGCTGGACTT CATGTTCGAG CGGTTCTCAT GACAACATAC AAACAATACA GCACCAGAGA 
AGCTGGGAGA CTCTGCCTTT TGGACATGCT CATTTCGATA GTTCAGGCGA TGGGGCAGGA 
CTGTGGGCCT CGGGCAGTCA TATGGACCAG CTGATGTTCC CTGATCATGG CACAAAGTAT 
GGCAGGCAAA GTCAAGGTAG GGAGGGCCTT GATCAAGCAC AGTCCAGAGC AAGCTGGGCA 
TCCTCGACAG GATACTGGGG AGAGGACTCG GAAGGTGATA CAGGTACCAT AAAGCGGAGG 
GGTGGGAAGG ATGTTTCAAT TGAAGCTGAA ACCAGTAGCA TAACATCTGT ACCAGCAGAG 
GAGTCAAAGC CAGCTCCCAT GCCCACTCAT ATAGCAGTAT CATCAAGTAG TACAAAGGGG 
CTTATTGCAC GAAAAGAGGG TCGATACCGG GAACCACCTC CAACTCCTCC TGGTTACGTA 
GGAATACCTA TTGCTGAGTT TGCAGAAGGT GGTTCCCATC CAACCAGAAA GCCTCCAGAT 
TACAACATAG CACTTCAGAG GTCTAGAATG GTGGCGCGGA CATGTGAGAC TCATGGGACA 
TCAACTCCAC AGCAGCAGTC ACACGGCCAT TCAACTAGCA GGCCTGTGAA CAAACCCCAG 
TGGCATAAAC CAAATGAGTC AGATCCACGT CTTGCTCACT ATCCATCTCA AGGGTTTTCC 
ACAGAGGAAG ATGAAGATGA ACAAGTCTCT GCTGTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011572573.1
CDS	30..4868
Translation	MEALCNLREHQLRLMCETVRYERHEANEVLYYPDDIGTCWYILLSGSVFIKESMFLPRSSFGKRSAGSFRRGCECIVLEPSEMIVVDYMDENEEYFQRQASHRQSRRRFRKINQKGERQTIIDTVDPYPVGKPPLPRGYHTLPADFTKLHLTDSLHPQVTHVSSSHSGCSITSDSGSSSLSDIYQATESEAGDMDLSGLPETAVDSEDDDDEEDIERASDPLMSRDIVRDCLEKDPIDRTDDDIEQLLEFMHQLPAFANMTMSVRRELCAVMVFAVVERAGTIVLNDGEELDSWSVILNGSVEVTYPDGRTEILCMGNSFGVSPTMEKEYMKGVMRTKVDDCQFVCIAQQDYCRILNQVEKNMQKVEEEGEIVMVKEHRELDRTGTRKGHIVIKGTAERLTMHLVEEHSVVDPTFIEDFLLTYRTFLSSPMEVGKKLLEWFNDPSLRDKVTRVVLLWVNNHFNDFEGDPAMTRFLEEFENNLEREKMGGHLRLLNIACAAKAKRRLITLTKPSREAPLPFILLGGSEKGFGIFVDSVDFGSKATEAGLKRGDQILEVNGQNFENIQLSKAMEILRNNTHLSITVKTNLFVFKELLTRLSEEKRNGAPHLPKIGDIKKASRYSIPDLAVDVEQVIGLEKVNKKSKANTVGGRNKLKKILDKTRISILPQKPYNDIGIGQSQDDSIVGLRQTKHIPPALPVSGTLSSSNPDLLQSHHRILDFNTTPDLPDQVLRVFKADQQSRYIMISKDTTAKEVVIQAIREFALTATPDAYSLCEVSVTPEGVIKQRRLPDQLSKLADRIQLSGRYYLKNNMETETLCSDEDAQELLRESQISLLQLSTVEVATQLSMRNFELFRNIEPTEYIDDLFKLKSKTGCTNLKKFEEVINQETFWVASEILRETNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLAPVARLRTTWEKLPSKYEKLFQDLQDLFDPSRNMAKYRNVLNSQNLQPPIIPLFPVIKKDLTFLHEGNDSKVEGLVNFEKLRMIAKEIRHVGRMASVNMDPALMFRTRKKKWRSLGSLSQGSTNAAVLDVAQTGGHKKRVRRSSFLNAKKLYEDAQMARKVKQYLSNLDLEMDEESLQTLSLQCEPATNTLPKNTGDKRSGKSETSPVAPRAGIQQKVQQQQQHKVNQALQVPAVSLYPSRKKVPVKDLPPFGINSPQALKKILSLSEEGSLDRHKKQSEDTVSSASSQLSSPPTSPQSSPRKGYTLAPSGTVDNFSDSGHSEISSRSSIVSNSSFDSMPVSLHDERRQRHSVSIVETNLGVGRIDRRIMIEPDQYSLGSYAPLSETRGLYAGATVLSSPSTEELSQDQGDRASLDAADSGRGSWTSCSSGSHDNIQTIQHQRSWETLPFGHAHFDSSGDGAGLWASGSHMDQLMFPDHGTKYGRQSQGREGLDQAQSRASWASSTGYWGEDSEGDTGTIKRRGGKDVSIEAETSSITSVPAEESKPAPMPTHIAVSSSSTKGLIARKEGRYREPPPTPPGYVGIPIAEFAEGGSHPTRKPPDYNIALQRSRMVARTCETHGTSTPQQQSHGHSTSRPVNKPQWHKPNESDPRLAHYPSQGFSTEEDEDEQVSAV

Target ORF information:

RefSeq Version	XM_011574271.1
Organism	Aquila chrysaetos canadensis
Definition	Aquila chrysaetos canadensis Rap guanine nucleotide exchange factor (GEF) 2 (RAPGEF2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011574271.1

ORF Insert Sequence:

ATGGAAGCCT TGTGTAATCT GAGAGAGCAT CAGCTAAGAC TGATGTGTGA AACTGTTAGA 
TATGAAAGGC ATGAAGCAAA TGAAGTTCTG TACTACCCTG ATGATATTGG TACCTGTTGG 
TATATCCTGC TGTCTGGTTC AGTCTTCATT AAAGAATCTA TGTTTCTTCC AAGGAGCAGT 
TTTGGCAAGC GTTCTGCAGG AAGCTTCAGG CGTGGCTGTG AATGCATTGT TTTAGAGCCA 
TCTGAAATGA TTGTGGTTGA TTACATGGAT GAAAATGAGG AGTATTTTCA GCGTCAAGCT 
TCTCATAGGC AATCTCGAAG AAGATTTAGG AAAATCAACC AAAAAGGGGA GCGACAAACA 
ATTATTGATA CTGTTGACCC TTATCCTGTG GGGAAGCCAC CTCTACCTAG AGGTTACCAT 
ACGCTTCCTG CAGATTTCAC AAAGCTGCAC CTTACTGACA GTCTCCACCC ACAGGTGACC 
CACGTTTCAT CTAGTCACTC AGGATGTAGC ATCACTAGTG ATTCAGGAAG CAGCAGCCTT 
TCTGATATCT ACCAGGCTAC AGAAAGTGAG GCTGGTGATA TGGATCTTAG TGGGTTGCCA 
GAGACAGCAG TGGATTCTGA GGATGATGAT GATGAAGAAG ACATTGAAAG GGCATCGGAT 
CCTTTGATGA GTAGGGATAT TGTTAGAGAC TGCTTGGAGA AAGACCCAAT AGACAGAACA 
GATGATGACA TTGAACAACT ACTGGAATTC ATGCATCAAT TGCCTGCTTT CGCCAACATG 
ACAATGTCAG TGAGGAGGGA GCTCTGTGCT GTGATGGTGT TTGCAGTTGT GGAGAGAGCA 
GGAACTATTG TACTGAACGA TGGGGAAGAG CTGGATTCCT GGTCAGTCAT TTTGAATGGC 
TCTGTGGAGG TGACGTACCC TGATGGAAGA ACAGAGATAC TCTGCATGGG GAACAGTTTT 
GGTGTTTCCC CTACCATGGA AAAGGAATAT ATGAAAGGAG TGATGAGAAC CAAAGTGGAT 
GACTGCCAGT TTGTTTGTAT AGCCCAGCAA GATTATTGCC GCATCCTCAA TCAAGTAGAA 
AAGAACATGC AGAAGGTAGA AGAGGAAGGG GAGATTGTGA TGGTGAAGGA GCACAGGGAG 
CTTGATCGCA CTGGAACAAG AAAAGGTCAC ATTGTCATCA AGGGAACAGC TGAAAGGTTA 
ACAATGCATT TGGTGGAAGA GCACTCTGTG GTAGACCCAA CATTTATAGA GGACTTCCTG 
TTGACATATC GGACCTTTCT TTCCAGCCCG ATGGAAGTGG GCAAGAAGTT GTTGGAGTGG 
TTCAATGACC CCAGCCTCAG GGATAAGGTT ACACGGGTAG TATTGTTGTG GGTGAACAAT 
CACTTCAATG ATTTTGAAGG AGATCCTGCT ATGACTCGAT TTTTGGAAGA ATTTGAGAAC 
AATTTGGAGA GGGAGAAAAT GGGTGGACAT TTGAGGCTGT TAAATATTGC TTGTGCTGCT 
AAAGCTAAAC GAAGATTGAT AACCTTAACA AAGCCATCTC GAGAAGCCCC TTTGCCTTTT 
ATCTTGCTGG GAGGGTCAGA AAAGGGATTT GGAATCTTTG TTGACAGTGT AGATTTTGGT 
AGCAAAGCCA CAGAAGCAGG CTTGAAACGT GGAGACCAGA TACTGGAAGT GAATGGTCAA 
AACTTTGAAA ACATTCAGCT GTCAAAAGCC ATGGAAATCC TTAGAAATAA CACTCATCTG 
TCTATCACTG TGAAAACAAA TTTATTTGTT TTTAAAGAAC TCTTAACACG GTTGTCAGAG 
GAAAAAAGAA ATGGTGCTCC CCACCTGCCT AAAATTGGTG ATATTAAAAA GGCGAGTCGT 
TATTCCATCC CAGACCTTGC TGTTGATGTG GAGCAGGTAA TAGGATTAGA AAAAGTAAAT 
AAGAAAAGTA AAGCCAACAC AGTTGGGGGA AGAAATAAAT TAAAGAAGAT ACTTGACAAG 
ACTCGAATCA GTATTCTGCC TCAGAAACCG TACAATGACA TTGGAATTGG CCAGTCTCAG 
GATGACAGCA TTGTGGGACT GAGGCAAACA AAGCACATTC CTCCTGCTTT ACCTGTCAGC 
GGAACCCTGT CATCCAGTAA TCCCGATCTA CTGCAGTCTC ATCACCGCAT CTTAGACTTC 
AATACTACTC CGGACTTACC AGATCAAGTT CTGAGGGTTT TCAAGGCAGA TCAGCAAAGT 
CGCTACATCA TGATCAGTAA GGACACAACA GCAAAAGAAG TGGTCATCCA GGCTATCAGG 
GAGTTTGCTC TCACTGCAAC CCCTGATGCA TATTCACTAT GCGAAGTGTC TGTCACACCT 
GAGGGTGTGA TCAAGCAAAG GAGGCTTCCT GATCAGTTAT CCAAGCTTGC TGACAGGATA 
CAGCTGAGTG GCAGGTATTA CCTGAAGAAC AACATGGAAA CAGAAACTCT TTGTTCAGAT 
GAAGATGCTC AAGAGTTACT AAGGGAAAGT CAAATTTCCC TATTACAGCT CAGTACTGTT 
GAGGTGGCTA CCCAACTCTC CATGAGAAAC TTCGAGCTAT TCCGTAATAT TGAACCCACG 
GAATACATAG ATGACTTGTT TAAACTCAAA TCAAAAACAG GTTGCACTAA TCTAAAAAAA 
TTTGAAGAGG TGATAAATCA AGAAACATTC TGGGTGGCTT CTGAGATTCT AAGAGAAACC 
AACCAGCTGA AAAGGATGAA GATCATTAAG CATTTCATTA AGATAGCACT ACACTGCAGA 
GAATGCAAGA ACTTCAACTC AATGTTTGCC ATCATTAGTG GCCTGAATTT GGCACCAGTT 
GCAAGGCTCC GAACTACTTG GGAAAAGCTT CCAAGCAAGT ATGAAAAACT GTTTCAAGAT 
CTACAGGACT TGTTTGATCC ATCTAGGAAT ATGGCAAAAT ATCGTAATGT CCTTAACAGC 
CAAAACCTAC AGCCCCCCAT TATACCCCTG TTTCCTGTTA TCAAAAAAGA CCTTACATTC 
CTTCATGAAG GAAATGATTC AAAAGTGGAG GGCTTGGTCA ATTTTGAGAA GCTGAGAATG 
ATTGCAAAGG AGATACGCCA TGTCGGTCGC ATGGCATCTG TGAACATGGA TCCTGCTTTA 
ATGTTTAGAA CAAGGAAGAA GAAATGGAGG AGTTTGGGGT CTCTCAGCCA GGGCAGTACA 
AATGCAGCTG TGCTGGATGT CGCGCAAACG GGGGGTCATA AAAAGCGGGT CCGTCGCAGC 
TCCTTCCTTA ATGCTAAAAA ACTGTATGAA GATGCTCAGA TGGCACGGAA AGTGAAGCAG 
TATCTCTCAA ACTTAGATCT GGAAATGGAT GAAGAGAGCC TCCAGACACT GTCTCTGCAG 
TGTGAGCCAG CCACCAACAC ACTGCCGAAG AATACAGGAG ACAAGCGATC TGGAAAGTCT 
GAAACGTCAC CGGTGGCTCC CCGGGCAGGC ATCCAGCAGA AAGTGCAGCA GCAGCAGCAA 
CATAAAGTAA ACCAAGCATT GCAGGTCCCT GCCGTATCTC TTTATCCCTC TCGCAAGAAA 
GTGCCGGTCA AAGACCTCCC ACCATTTGGC ATTAACTCCC CACAAGCTTT AAAGAAAATT 
CTTTCCTTAT CTGAAGAAGG AAGTTTGGAT CGTCACAAGA AACAATCTGA AGACACGGTT 
TCAAGTGCGT CTTCGCAGCT GTCTTCTCCT CCCACTTCAC CACAGAGCTC TCCAAGGAAA 
GGCTATACTT TGGCTCCTAG CGGCACAGTG GATAACTTTT CAGATTCTGG TCACAGCGAA 
ATTTCTTCCA GATCTAGTAT TGTCAGCAAT TCCTCTTTTG ACTCTATGCC AGTCTCCCTG 
CATGATGAGA GGAGACAGAG ACATTCTGTC AGCATCGTGG AGACAAATCT TGGTGTGGGA 
AGGATTGATA GAAGAATCAT GATTGAACCA GATCAATACA GCTTAGGCTC ATATGCACCA 
CTGTCAGAGA CCAGAGGCCT GTATGCTGGA GCAACTGTGC TTTCTTCTCC TAGTACAGAA 
GAACTGTCAC AGGATCAGGG GGACAGAGCT TCGCTCGATG CAGCTGACAG TGGCCGTGGT 
AGCTGGACTT CATGTTCGAG CGGTTCTCAT GACAACATAC AAACAATACA GCACCAGAGA 
AGCTGGGAGA CTCTGCCTTT TGGACATGCT CATTTCGATA GTTCAGGCGA TGGGGCAGGA 
CTGTGGGCCT CGGGCAGTCA TATGGACCAG CTGATGTTCC CTGATCATGG CACAAAGTAT 
GGCAGGCAAA GTCAAGGTAG GGAGGGCCTT GATCAAGCAC AGTCCAGAGC AAGCTGGGCA 
TCCTCGACAG GATACTGGGG AGAGGACTCG GAAGGTGATA CAGGTACCAT AAAGCGGAGG 
GGTGGGAAGG ATGTTTCAAT TGAAGCTGAA ACCAGTAGCA TAACATCTGT ACCAGCAGAG 
GAGTCAAAGC CAGCTCCCAT GCCCACTCAT ATAGCAGTAT CATCAAGTAG TACAAAGGGG 
CTTATTGCAC GAAAAGAGGG TCGATACCGG GAACCACCTC CAACTCCTCC TGGTTACGTA 
GGAATACCTA TTGCTGAGTT TGCAGAAGGT GGTTCCCATC CAACCAGAAA GCCTCCAGAT 
TACAACATAG CACTTCAGAG GTCTAGAATG GTGGCGCGGA CATGTGAGAC TCATGGGACA 
TCAACTCCAC AGCAGCAGTC ACACGGCCAT TCAACTAGCA GGCCTGTGAA CAAACCCCAG 
TGGCATAAAC CAAATGAGTC AGATCCACGT CTTGCTCACT ATCCATCTCA AGGGTTTTCC 
ACAGAGGAAG ATGAAGATGA ACAAGTCTCT GCTGTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAPGEF2 ( XM_011574272.1 ) cDNA ORF clone, Aquila chrysaetos canadensis -> XP_011572574.1 Aquila chrysaetos canadensis Rap guanine nucleotide exchange factor (GEF) 2 (RAPGEF2), transcript variant X3, mRNA.

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CloneID	OAq14196
Clone ID Related Accession (Same CDS sequence)	XM_011574272.1
Accession Version	XM_011574272.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4833bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-12
Organism	Aquila chrysaetos canadensis
Product	rap guanine nucleotide exchange factor 2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011950881.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Aquila chrysaetos canadensis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGCCT TGTGTAATCT GAGAGAGCAT CAGCTAAGAC TGATGTGTGA AACTGTTAGA 
TATGAAAGGC ATGAAGCAAA TGAAGTTCTG TACTACCCTG ATGATATTGG TACCTGTTGG 
TATATCCTGC TGTCTGGTTC AGTCTTCATT AAAGAATCTA TGTTTCTTCC AAGGAGCAGT 
TTTGGCAAGC GTTCTGCAGG AAGCTTCAGG CGTGGCTGTG AATGCATTGT TTTAGAGCCA 
TCTGAAATGA TTGTGGTTGA TTACATGGAT GAAAATGAGG AGTATTTTCA GCGTCAAGCT 
TCTCATAGGC AATCTCGAAG AAGATTTAGG AAAATCAACC AAAAAGGGGA GCGACAAACA 
ATTATTGATA CTGTTGACCC TTATCCTGTG GGGAAGCCAC CTCTACCTAG AGGTTACCAT 
ACGGAATGCA CTAAACCTCA GCTTCCTGCA GATTTCACAA AGCTGCACCT TACTGACAGT 
CTCCACCCAC AGGTGACCCA CGTTTCATCT AGTCACTCAG GATGTAGCAT CACTAGTGAT 
TCAGGAAGCA GCAGCCTTTC TGATATCTAC CAGGCTACAG AAAGTGAGGC TGGTGATATG 
GATCTTAGTG GGTTGCCAGA GACAGCAGTG GATTCTGAGG ATGATGATGA TGAAGAAGAC 
ATTGAAAGGG CATCGGATCC TTTGATGAGT AGGGATATTG TTAGAGACTG CTTGGAGAAA 
GACCCAATAG ACAGAACAGA TGATGACATT GAACAACTAC TGGAATTCAT GCATCAATTG 
CCTGCTTTCG CCAACATGAC AATGTCAGTG AGGAGGGAGC TCTGTGCTGT GATGGTGTTT 
GCAGTTGTGG AGAGAGCAGG AACTATTGTA CTGAACGATG GGGAAGAGCT GGATTCCTGG 
TCAGTCATTT TGAATGGCTC TGTGGAGGTG ACGTACCCTG ATGGAAGAAC AGAGATACTC 
TGCATGGGGA ACAGTTTTGG TGTTTCCCCT ACCATGGAAA AGGAATATAT GAAAGGAGTG 
ATGAGAACCA AAGTGGATGA CTGCCAGTTT GTTTGTATAG CCCAGCAAGA TTATTGCCGC 
ATCCTCAATC AAGTAGAAAA GAACATGCAG AAGGTAGAAG AGGAAGGGGA GATTGTGATG 
GTGAAGGAGC ACAGGGAGCT TGATCGCACT GGAACAAGAA AAGGTCACAT TGTCATCAAG 
GGAACAGCTG AAAGGTTAAC AATGCATTTG GTGGAAGAGC ACTCTGTGGT AGACCCAACA 
TTTATAGAGG ACTTCCTGTT GACATATCGG ACCTTTCTTT CCAGCCCGAT GGAAGTGGGC 
AAGAAGTTGT TGGAGTGGTT CAATGACCCC AGCCTCAGGG ATAAGGTTAC ACGGGTAGTA 
TTGTTGTGGG TGAACAATCA CTTCAATGAT TTTGAAGGAG ATCCTGCTAT GACTCGATTT 
TTGGAAGAAT TTGAGAACAA TTTGGAGAGG GAGAAAATGG GTGGACATTT GAGGCTGTTA 
AATATTGCTT GTGCTGCTAA AGCTAAACGA AGATTGATAA CCTTAACAAA GCCATCTCGA 
GAAGCCCCTT TGCCTTTTAT CTTGCTGGGA GGGTCAGAAA AGGGATTTGG AATCTTTGTT 
GACAGTGTAG ATTTTGGTAG CAAAGCCACA GAAGCAGGCT TGAAACGTGG AGACCAGATA 
CTGGAAGTGA ATGGTCAAAA CTTTGAAAAC ATTCAGCTGT CAAAAGCCAT GGAAATCCTT 
AGAAATAACA CTCATCTGTC TATCACTGTG AAAACAAATT TATTTGTTTT TAAAGAACTC 
TTAACACGGT TGTCAGAGGA AAAAAGAAAT GGTGCTCCCC ACCTGCCTAA AATTGGTGAT 
ATTAAAAAGG CGAGTCGTTA TTCCATCCCA GACCTTGCTG TTGATGTGGA GCAGGTAATA 
GGATTAGAAA AAGTAAATAA GAAAAGTAAA GCCAACACAG TTGGGGGAAG AAATAAATTA 
AAGAAGATAC TTGACAAGAC TCGAATCAGT ATTCTGCCTC AGAAACCGTA CAATGACATT 
GGAATTGGCC AGTCTCAGGA TGACAGCATT GTGGGACTGA GGCAAACAAA GCACATTCCT 
CCTGCTTTAC CTGTCAGCGG AACCCTGTCA TCCAGTAATC CCGATCTACT GCAGTCTCAT 
CACCGCATCT TAGACTTCAA TACTACTCCG GACTTACCAG ATCAAGTTCT GAGGGTTTTC 
AAGGCAGATC AGCAAAGTCG CTACATCATG ATCAGTAAGG ACACAACAGC AAAAGAAGTG 
GTCATCCAGG CTATCAGGGA GTTTGCTCTC ACTGCAACCC CTGATGCATA TTCACTATGC 
GAAGTGTCTG TCACACCTGA GGGTGTGATC AAGCAAAGGA GGCTTCCTGA TCAGTTATCC 
AAGCTTGCTG ACAGGATACA GCTGAGTGGC AGGTATTACC TGAAGAACAA CATGGAAACA 
GAAACTCTTT GTTCAGATGA AGATGCTCAA GAGTTACTAA GGGAAAGTCA AATTTCCCTA 
TTACAGCTCA GTACTGTTGA GGTGGCTACC CAACTCTCCA TGAGAAACTT CGAGCTATTC 
CGTAATATTG AACCCACGGA ATACATAGAT GACTTGTTTA AACTCAAATC AAAAACAGGT 
TGCACTAATC TAAAAAAATT TGAAGAGGTG ATAAATCAAG AAACATTCTG GGTGGCTTCT 
GAGATTCTAA GAGAAACCAA CCAGCTGAAA AGGATGAAGA TCATTAAGCA TTTCATTAAG 
ATAGCACTAC ACTGCAGAGA ATGCAAGAAC TTCAACTCAA TGTTTGCCAT CATTAGTGGC 
CTGAATTTGG CACCAGTTGC AAGGCTCCGA ACTACTTGGG AAAAGCTTCC AAGCAAGTAT 
GAAAAACTGT TTCAAGATCT ACAGGACTTG TTTGATCCAT CTAGGAATAT GGCAAAATAT 
CGTAATGTCC TTAACAGCCA AAACCTACAG CCCCCCATTA TACCCCTGTT TCCTGTTATC 
AAAAAAGACC TTACATTCCT TCATGAAGGA AATGATTCAA AAGTGGAGGG CTTGGTCAAT 
TTTGAGAAGC TGAGAATGAT TGCAAAGGAG ATACGCCATG TCGGTCGCAT GGCATCTGTG 
AACATGGATC CTGCTTTAAT GTTTAGAACA AGGTCTCTCA GCCAGGGCAG TACAAATGCA 
GCTGTGCTGG ATGTCGCGCA AACGGGGGGT CATAAAAAGC GGGTCCGTCG CAGCTCCTTC 
CTTAATGCTA AAAAACTGTA TGAAGATGCT CAGATGGCAC GGAAAGTGAA GCAGTATCTC 
TCAAACTTAG ATCTGGAAAT GGATGAAGAG AGCCTCCAGA CACTGTCTCT GCAGTGTGAG 
CCAGCCACCA ACACACTGCC GAAGAATACA GGAGACAAGC GATCTGGAAA GTCTGAAACG 
TCACCGGTGG CTCCCCGGGC AGGCATCCAG CAGAAAGTGC AGCAGCAGCA GCAACATAAA 
GTAAACCAAG CATTGCAGGT CCCTGCCGTA TCTCTTTATC CCTCTCGCAA GAAAGTGCCG 
GTCAAAGACC TCCCACCATT TGGCATTAAC TCCCCACAAG CTTTAAAGAA AATTCTTTCC 
TTATCTGAAG AAGGAAGTTT GGATCGTCAC AAGAAACAAT CTGAAGACAC GGTTTCAAGT 
GCGTCTTCGC AGCTGTCTTC TCCTCCCACT TCACCACAGA GCTCTCCAAG GAAAGGCTAT 
ACTTTGGCTC CTAGCGGCAC AGTGGATAAC TTTTCAGATT CTGGTCACAG CGAAATTTCT 
TCCAGATCTA GTATTGTCAG CAATTCCTCT TTTGACTCTA TGCCAGTCTC CCTGCATGAT 
GAGAGGAGAC AGAGACATTC TGTCAGCATC GTGGAGACAA ATCTTGGTGT GGGAAGGATT 
GATAGAAGAA TCATGATTGA ACCAGATCAA TACAGCTTAG GCTCATATGC ACCACTGTCA 
GAGACCAGAG GCCTGTATGC TGGAGCAACT GTGCTTTCTT CTCCTAGTAC AGAAGAACTG 
TCACAGGATC AGGGGGACAG AGCTTCGCTC GATGCAGCTG ACAGTGGCCG TGGTAGCTGG 
ACTTCATGTT CGAGCGGTTC TCATGACAAC ATACAAACAA TACAGCACCA GAGAAGCTGG 
GAGACTCTGC CTTTTGGACA TGCTCATTTC GATAGTTCAG GCGATGGGGC AGGACTGTGG 
GCCTCGGGCA GTCATATGGA CCAGCTGATG TTCCCTGATC ATGGCACAAA GTATGGCAGG 
CAAAGTCAAG GTAGGGAGGG CCTTGATCAA GCACAGTCCA GAGCAAGCTG GGCATCCTCG 
ACAGGATACT GGGGAGAGGA CTCGGAAGGT GATACAGGTA CCATAAAGCG GAGGGGTGGG 
AAGGATGTTT CAATTGAAGC TGAAACCAGT AGCATAACAT CTGTACCAGC AGAGGAGTCA 
AAGCCAGCTC CCATGCCCAC TCATATAGCA GTATCATCAA GTAGTACAAA GGGGCTTATT 
GCACGAAAAG AGGGTCGATA CCGGGAACCA CCTCCAACTC CTCCTGGTTA CGTAGGAATA 
CCTATTGCTG AGTTTGCAGA AGGTGGTTCC CATCCAACCA GAAAGCCTCC AGATTACAAC 
ATAGCACTTC AGAGGTCTAG AATGGTGGCG CGGACATGTG AGACTCATGG GACATCAACT 
CCACAGCAGC AGTCACACGG CCATTCAACT AGCAGGCCTG TGAACAAACC CCAGTGGCAT 
AAACCAAATG AGTCAGATCC ACGTCTTGCT CACTATCCAT CTCAAGGGTT TTCCACAGAG 
GAAGATGAAG ATGAACAAGT CTCTGCTGTC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011572574.1
CDS	30..4862
Translation	MEALCNLREHQLRLMCETVRYERHEANEVLYYPDDIGTCWYILLSGSVFIKESMFLPRSSFGKRSAGSFRRGCECIVLEPSEMIVVDYMDENEEYFQRQASHRQSRRRFRKINQKGERQTIIDTVDPYPVGKPPLPRGYHTECTKPQLPADFTKLHLTDSLHPQVTHVSSSHSGCSITSDSGSSSLSDIYQATESEAGDMDLSGLPETAVDSEDDDDEEDIERASDPLMSRDIVRDCLEKDPIDRTDDDIEQLLEFMHQLPAFANMTMSVRRELCAVMVFAVVERAGTIVLNDGEELDSWSVILNGSVEVTYPDGRTEILCMGNSFGVSPTMEKEYMKGVMRTKVDDCQFVCIAQQDYCRILNQVEKNMQKVEEEGEIVMVKEHRELDRTGTRKGHIVIKGTAERLTMHLVEEHSVVDPTFIEDFLLTYRTFLSSPMEVGKKLLEWFNDPSLRDKVTRVVLLWVNNHFNDFEGDPAMTRFLEEFENNLEREKMGGHLRLLNIACAAKAKRRLITLTKPSREAPLPFILLGGSEKGFGIFVDSVDFGSKATEAGLKRGDQILEVNGQNFENIQLSKAMEILRNNTHLSITVKTNLFVFKELLTRLSEEKRNGAPHLPKIGDIKKASRYSIPDLAVDVEQVIGLEKVNKKSKANTVGGRNKLKKILDKTRISILPQKPYNDIGIGQSQDDSIVGLRQTKHIPPALPVSGTLSSSNPDLLQSHHRILDFNTTPDLPDQVLRVFKADQQSRYIMISKDTTAKEVVIQAIREFALTATPDAYSLCEVSVTPEGVIKQRRLPDQLSKLADRIQLSGRYYLKNNMETETLCSDEDAQELLRESQISLLQLSTVEVATQLSMRNFELFRNIEPTEYIDDLFKLKSKTGCTNLKKFEEVINQETFWVASEILRETNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLAPVARLRTTWEKLPSKYEKLFQDLQDLFDPSRNMAKYRNVLNSQNLQPPIIPLFPVIKKDLTFLHEGNDSKVEGLVNFEKLRMIAKEIRHVGRMASVNMDPALMFRTRSLSQGSTNAAVLDVAQTGGHKKRVRRSSFLNAKKLYEDAQMARKVKQYLSNLDLEMDEESLQTLSLQCEPATNTLPKNTGDKRSGKSETSPVAPRAGIQQKVQQQQQHKVNQALQVPAVSLYPSRKKVPVKDLPPFGINSPQALKKILSLSEEGSLDRHKKQSEDTVSSASSQLSSPPTSPQSSPRKGYTLAPSGTVDNFSDSGHSEISSRSSIVSNSSFDSMPVSLHDERRQRHSVSIVETNLGVGRIDRRIMIEPDQYSLGSYAPLSETRGLYAGATVLSSPSTEELSQDQGDRASLDAADSGRGSWTSCSSGSHDNIQTIQHQRSWETLPFGHAHFDSSGDGAGLWASGSHMDQLMFPDHGTKYGRQSQGREGLDQAQSRASWASSTGYWGEDSEGDTGTIKRRGGKDVSIEAETSSITSVPAEESKPAPMPTHIAVSSSSTKGLIARKEGRYREPPPTPPGYVGIPIAEFAEGGSHPTRKPPDYNIALQRSRMVARTCETHGTSTPQQQSHGHSTSRPVNKPQWHKPNESDPRLAHYPSQGFSTEEDEDEQVSAV

Target ORF information:

RefSeq Version	XM_011574272.1
Organism	Aquila chrysaetos canadensis
Definition	Aquila chrysaetos canadensis Rap guanine nucleotide exchange factor (GEF) 2 (RAPGEF2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011574272.1

ORF Insert Sequence:

ATGGAAGCCT TGTGTAATCT GAGAGAGCAT CAGCTAAGAC TGATGTGTGA AACTGTTAGA 
TATGAAAGGC ATGAAGCAAA TGAAGTTCTG TACTACCCTG ATGATATTGG TACCTGTTGG 
TATATCCTGC TGTCTGGTTC AGTCTTCATT AAAGAATCTA TGTTTCTTCC AAGGAGCAGT 
TTTGGCAAGC GTTCTGCAGG AAGCTTCAGG CGTGGCTGTG AATGCATTGT TTTAGAGCCA 
TCTGAAATGA TTGTGGTTGA TTACATGGAT GAAAATGAGG AGTATTTTCA GCGTCAAGCT 
TCTCATAGGC AATCTCGAAG AAGATTTAGG AAAATCAACC AAAAAGGGGA GCGACAAACA 
ATTATTGATA CTGTTGACCC TTATCCTGTG GGGAAGCCAC CTCTACCTAG AGGTTACCAT 
ACGGAATGCA CTAAACCTCA GCTTCCTGCA GATTTCACAA AGCTGCACCT TACTGACAGT 
CTCCACCCAC AGGTGACCCA CGTTTCATCT AGTCACTCAG GATGTAGCAT CACTAGTGAT 
TCAGGAAGCA GCAGCCTTTC TGATATCTAC CAGGCTACAG AAAGTGAGGC TGGTGATATG 
GATCTTAGTG GGTTGCCAGA GACAGCAGTG GATTCTGAGG ATGATGATGA TGAAGAAGAC 
ATTGAAAGGG CATCGGATCC TTTGATGAGT AGGGATATTG TTAGAGACTG CTTGGAGAAA 
GACCCAATAG ACAGAACAGA TGATGACATT GAACAACTAC TGGAATTCAT GCATCAATTG 
CCTGCTTTCG CCAACATGAC AATGTCAGTG AGGAGGGAGC TCTGTGCTGT GATGGTGTTT 
GCAGTTGTGG AGAGAGCAGG AACTATTGTA CTGAACGATG GGGAAGAGCT GGATTCCTGG 
TCAGTCATTT TGAATGGCTC TGTGGAGGTG ACGTACCCTG ATGGAAGAAC AGAGATACTC 
TGCATGGGGA ACAGTTTTGG TGTTTCCCCT ACCATGGAAA AGGAATATAT GAAAGGAGTG 
ATGAGAACCA AAGTGGATGA CTGCCAGTTT GTTTGTATAG CCCAGCAAGA TTATTGCCGC 
ATCCTCAATC AAGTAGAAAA GAACATGCAG AAGGTAGAAG AGGAAGGGGA GATTGTGATG 
GTGAAGGAGC ACAGGGAGCT TGATCGCACT GGAACAAGAA AAGGTCACAT TGTCATCAAG 
GGAACAGCTG AAAGGTTAAC AATGCATTTG GTGGAAGAGC ACTCTGTGGT AGACCCAACA 
TTTATAGAGG ACTTCCTGTT GACATATCGG ACCTTTCTTT CCAGCCCGAT GGAAGTGGGC 
AAGAAGTTGT TGGAGTGGTT CAATGACCCC AGCCTCAGGG ATAAGGTTAC ACGGGTAGTA 
TTGTTGTGGG TGAACAATCA CTTCAATGAT TTTGAAGGAG ATCCTGCTAT GACTCGATTT 
TTGGAAGAAT TTGAGAACAA TTTGGAGAGG GAGAAAATGG GTGGACATTT GAGGCTGTTA 
AATATTGCTT GTGCTGCTAA AGCTAAACGA AGATTGATAA CCTTAACAAA GCCATCTCGA 
GAAGCCCCTT TGCCTTTTAT CTTGCTGGGA GGGTCAGAAA AGGGATTTGG AATCTTTGTT 
GACAGTGTAG ATTTTGGTAG CAAAGCCACA GAAGCAGGCT TGAAACGTGG AGACCAGATA 
CTGGAAGTGA ATGGTCAAAA CTTTGAAAAC ATTCAGCTGT CAAAAGCCAT GGAAATCCTT 
AGAAATAACA CTCATCTGTC TATCACTGTG AAAACAAATT TATTTGTTTT TAAAGAACTC 
TTAACACGGT TGTCAGAGGA AAAAAGAAAT GGTGCTCCCC ACCTGCCTAA AATTGGTGAT 
ATTAAAAAGG CGAGTCGTTA TTCCATCCCA GACCTTGCTG TTGATGTGGA GCAGGTAATA 
GGATTAGAAA AAGTAAATAA GAAAAGTAAA GCCAACACAG TTGGGGGAAG AAATAAATTA 
AAGAAGATAC TTGACAAGAC TCGAATCAGT ATTCTGCCTC AGAAACCGTA CAATGACATT 
GGAATTGGCC AGTCTCAGGA TGACAGCATT GTGGGACTGA GGCAAACAAA GCACATTCCT 
CCTGCTTTAC CTGTCAGCGG AACCCTGTCA TCCAGTAATC CCGATCTACT GCAGTCTCAT 
CACCGCATCT TAGACTTCAA TACTACTCCG GACTTACCAG ATCAAGTTCT GAGGGTTTTC 
AAGGCAGATC AGCAAAGTCG CTACATCATG ATCAGTAAGG ACACAACAGC AAAAGAAGTG 
GTCATCCAGG CTATCAGGGA GTTTGCTCTC ACTGCAACCC CTGATGCATA TTCACTATGC 
GAAGTGTCTG TCACACCTGA GGGTGTGATC AAGCAAAGGA GGCTTCCTGA TCAGTTATCC 
AAGCTTGCTG ACAGGATACA GCTGAGTGGC AGGTATTACC TGAAGAACAA CATGGAAACA 
GAAACTCTTT GTTCAGATGA AGATGCTCAA GAGTTACTAA GGGAAAGTCA AATTTCCCTA 
TTACAGCTCA GTACTGTTGA GGTGGCTACC CAACTCTCCA TGAGAAACTT CGAGCTATTC 
CGTAATATTG AACCCACGGA ATACATAGAT GACTTGTTTA AACTCAAATC AAAAACAGGT 
TGCACTAATC TAAAAAAATT TGAAGAGGTG ATAAATCAAG AAACATTCTG GGTGGCTTCT 
GAGATTCTAA GAGAAACCAA CCAGCTGAAA AGGATGAAGA TCATTAAGCA TTTCATTAAG 
ATAGCACTAC ACTGCAGAGA ATGCAAGAAC TTCAACTCAA TGTTTGCCAT CATTAGTGGC 
CTGAATTTGG CACCAGTTGC AAGGCTCCGA ACTACTTGGG AAAAGCTTCC AAGCAAGTAT 
GAAAAACTGT TTCAAGATCT ACAGGACTTG TTTGATCCAT CTAGGAATAT GGCAAAATAT 
CGTAATGTCC TTAACAGCCA AAACCTACAG CCCCCCATTA TACCCCTGTT TCCTGTTATC 
AAAAAAGACC TTACATTCCT TCATGAAGGA AATGATTCAA AAGTGGAGGG CTTGGTCAAT 
TTTGAGAAGC TGAGAATGAT TGCAAAGGAG ATACGCCATG TCGGTCGCAT GGCATCTGTG 
AACATGGATC CTGCTTTAAT GTTTAGAACA AGGTCTCTCA GCCAGGGCAG TACAAATGCA 
GCTGTGCTGG ATGTCGCGCA AACGGGGGGT CATAAAAAGC GGGTCCGTCG CAGCTCCTTC 
CTTAATGCTA AAAAACTGTA TGAAGATGCT CAGATGGCAC GGAAAGTGAA GCAGTATCTC 
TCAAACTTAG ATCTGGAAAT GGATGAAGAG AGCCTCCAGA CACTGTCTCT GCAGTGTGAG 
CCAGCCACCA ACACACTGCC GAAGAATACA GGAGACAAGC GATCTGGAAA GTCTGAAACG 
TCACCGGTGG CTCCCCGGGC AGGCATCCAG CAGAAAGTGC AGCAGCAGCA GCAACATAAA 
GTAAACCAAG CATTGCAGGT CCCTGCCGTA TCTCTTTATC CCTCTCGCAA GAAAGTGCCG 
GTCAAAGACC TCCCACCATT TGGCATTAAC TCCCCACAAG CTTTAAAGAA AATTCTTTCC 
TTATCTGAAG AAGGAAGTTT GGATCGTCAC AAGAAACAAT CTGAAGACAC GGTTTCAAGT 
GCGTCTTCGC AGCTGTCTTC TCCTCCCACT TCACCACAGA GCTCTCCAAG GAAAGGCTAT 
ACTTTGGCTC CTAGCGGCAC AGTGGATAAC TTTTCAGATT CTGGTCACAG CGAAATTTCT 
TCCAGATCTA GTATTGTCAG CAATTCCTCT TTTGACTCTA TGCCAGTCTC CCTGCATGAT 
GAGAGGAGAC AGAGACATTC TGTCAGCATC GTGGAGACAA ATCTTGGTGT GGGAAGGATT 
GATAGAAGAA TCATGATTGA ACCAGATCAA TACAGCTTAG GCTCATATGC ACCACTGTCA 
GAGACCAGAG GCCTGTATGC TGGAGCAACT GTGCTTTCTT CTCCTAGTAC AGAAGAACTG 
TCACAGGATC AGGGGGACAG AGCTTCGCTC GATGCAGCTG ACAGTGGCCG TGGTAGCTGG 
ACTTCATGTT CGAGCGGTTC TCATGACAAC ATACAAACAA TACAGCACCA GAGAAGCTGG 
GAGACTCTGC CTTTTGGACA TGCTCATTTC GATAGTTCAG GCGATGGGGC AGGACTGTGG 
GCCTCGGGCA GTCATATGGA CCAGCTGATG TTCCCTGATC ATGGCACAAA GTATGGCAGG 
CAAAGTCAAG GTAGGGAGGG CCTTGATCAA GCACAGTCCA GAGCAAGCTG GGCATCCTCG 
ACAGGATACT GGGGAGAGGA CTCGGAAGGT GATACAGGTA CCATAAAGCG GAGGGGTGGG 
AAGGATGTTT CAATTGAAGC TGAAACCAGT AGCATAACAT CTGTACCAGC AGAGGAGTCA 
AAGCCAGCTC CCATGCCCAC TCATATAGCA GTATCATCAA GTAGTACAAA GGGGCTTATT 
GCACGAAAAG AGGGTCGATA CCGGGAACCA CCTCCAACTC CTCCTGGTTA CGTAGGAATA 
CCTATTGCTG AGTTTGCAGA AGGTGGTTCC CATCCAACCA GAAAGCCTCC AGATTACAAC 
ATAGCACTTC AGAGGTCTAG AATGGTGGCG CGGACATGTG AGACTCATGG GACATCAACT 
CCACAGCAGC AGTCACACGG CCATTCAACT AGCAGGCCTG TGAACAAACC CCAGTGGCAT 
AAACCAAATG AGTCAGATCC ACGTCTTGCT CACTATCCAT CTCAAGGGTT TTCCACAGAG 
GAAGATGAAG ATGAACAAGT CTCTGCTGTC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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03-6811-6572

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オンライン注文

RAPGEF2 cDNA ORF clone, Aquila chrysaetos canadensis

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム