PDE5A cDNA ORF clone, Apteryx australis mantelli

Gene
Clones
gRNAs

Summary

Gene Symbol	PDE5A
Entrez Gene ID	106491978
Full Name	phosphodiesterase 5A
Gene Type	protein-coding
Organism	Apteryx australis mantelli

mRNA and Protein(s)

mRNA	Protein	Name
XM_013951652.1	XP_013807106.1	cGMP-specific 3',5'-cyclic phosphodiesterase

Pathways from BioSystems
Gene Ontology

The following PDE5A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PDE5A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAp20853	XM_013951652.1 Latest version!	Apteryx australis mantelli phosphodiesterase 5A, cGMP-specific (PDE5A), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PDE5A ( XM_013951652.1 ) cDNA ORF clone, Apteryx australis mantelli -> XP_013807106.1 Apteryx australis mantelli phosphodiesterase 5A, cGMP-specific (PDE5A), mRNA.

Price & Availability↑

CloneID	OAp20853
Clone ID Related Accession (Same CDS sequence)	XM_013951652.1
Accession Version	XM_013951652.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2463bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-08
Organism	Apteryx australis mantelli
Product	cGMP-specific 3',5'-cyclic phosphodiesterase
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014000569.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Apteryx australis mantelli Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTTAATG CATGGTTTGC TGAGAGAGTT CACACCATCC CAGTCTGCAA GGAGGGAACC 
AAGACCCAGA GTGAATCCTG CTCTTGTCAC CAGCCTGCCT GTGCTGAGAC CACCAACGCT 
GGGACTCCAG CGAGGAAAAT CTCTGCTTCT GAATTTGACA GACCCTTAAG GCCCATTTTT 
GTCAAGGACT CAGTAGGAGC AGTGAGCTTC CTCTCTGACT CTGAAAAGAA GGAACAGATG 
CCTCTGCAAT CTCCACGGAT TGGGACCAGC GCAGGGGATC AGTGCTCAAG ACTCTTAGAA 
CTTGTGAAAG ATATTTCTAG TCATTTGGAT GTCACAGCGC TTTGCCACAA AATTTTCCTA 
CATATCCATG AGCTTATAGC AGCTGATCGT TATTCCCTGT TCTTGGTCTG TGAGGACAGC 
TCTAACGAGA AGTTTCTTGT AAGCAGGCTG TTTGATGTGG CAGAAGGCTC CACCCTGGAA 
GAAGCTTCAA ACAACTGCAT TCGCCTGGAG TGGAACAAGG GCATTGTGGG TCACGTAGCA 
GCTATAGGCC AGCCTCTGAA CATCAAGAAC GCTTATGAGG ATCCAAGATT CAATGCAGAA 
GTTGACCAGA TCACCGGCTA TAAGACTCAG AGTATTCTGT GTATGCCAAT AAAAAATCAC 
AGGGAAGAGG TTGTTGGTGT GGCTCAAGCT ATCAATAAAA AATCTGGAAT TGGTGGGACT 
TTCACTGAAC AAGATGAAAA GGATTTTGCT GCTTATCTGG CATTTTGTGG CATTGTTCTT 
CACAACGCTC AGTTGTATGA GACATCTTTG CTTGAAAACA GACGTAACCA GGTGCTTCTT 
GATCTTGCCA GCCTGATTTT TGAAGAGCAG CAGTCTTTGG AAGTAATTTT GAAGAAGATA 
GCTGCCACTA TAATATCCTT CATGCAAGTG CAGAGGTGTA CCATTTTTAT AGTGGATGAA 
GATTGTACTG ATTCATTTTC TAGTGTGTTT CACATGGAAT CTGAGGAATT AGAAGATTCA 
GCTGAAAACC TGAAGAGGGA CTATGATACA AACAAAATCA ATTATATGTA TGCTGAATAT 
GTCAAGAATA CAATGGAGCC CCTCAACATC CCAGATGTCT GCAAAGATAG AAGATTTCCC 
TGGACAAATG ACAATGCAGA AAATGTAAGC CAAAACATAA AGAGTTTGCT GTGTACACCA 
ATCAAAAACG GGAAAAAGAA TAAGGTGATA GGGGTTTGTC AGCTGGTGAA TAAGATGGAA 
GAAAACTCAG GCAAAATCAA GGCTTTCAAC AGAAATGATG AACAGTTCCT GGAAGCATTT 
GTCATCTTTT GTGGCTTGGG AATCCAGAAT ACACAGATGT ATGAAGCTGT AGAAAGAGCA 
ATGGCCAAGC AGATGGTGAC ATTAGAGGTT CTCTCGTACC ATGCTTCTGC TGCTGAGGAG 
GAAACGAGGG AGCTGCAGGT AACAGCGGCT GCTGTAGTAC CATCTGCACA ATCTCTCAAC 
CTAACTGACT TCAACTTCAG TGATTTTGAG CTATCAGATT TTGAAACAAC ACTGTGTACA 
ATTCGAATGT TCACAGACCT CAACCTGGTG CAAAATTTCC AAATGAAGCA TGAGGTTCTC 
TGCAGATGGA TTTTAAGTGT AAAGAAAAAT TATCGGAAAA ATGTTGCTTA TCACAATTGG 
AGACATGCCT TTAATACGGC CCAGTGCATG TTTGCTGCTT TAAAATCTGG TAAAATTCAG 
AGCAAACTGA CTGACTTAGA AACACTTGCT TTGCTGATAG CAGCTCTAAG CCATGATTTG 
GATCACAGAG GAGTGAACAA CTCTTACATA CAAAGAAGTG AACATCCACT GGCTCAACTG 
TATTGCCACT CAATCATGGA GCATCACCAT TTCGATCAAT GCCTTATGAT CCTGAATAGT 
CCAGGAAATC AGATTCTCAG CAGCCTTTCC ATTGAAGAAT ACAAGGCCAC ACTGAAAATG 
ATAAAACAAG CCATTCTTGC CACAGACCTA GCACTATATA TAAAGAGGAG GGGAGAGTTT 
TTTGAACTTC TAAGGAAGAA GCAATTTAAT TGGGAAGATC CTATGCAGAA GGAGCTATTT 
TTAGCAATGC TGATGACTGC TTGTGATTTA TCAGCCATAA CCAAACCATG GCCAGTTCAG 
CAACGGATTG CTGAGCTTGT AGCTACAGAG TTCTTTGACC AAGGAGATAA AGAGCGGAAA 
GAACTCAACA TAGAACCAAC AGATCTAATG AACAGAGAGA AGAAAAATAA AATTCCCAGC 
ATGCAGGTTG GATTCATAGA TGCTGTTTGT TTACAGCTGT ATGAGGCCCT AACACACGTA 
TCAGAGGCCT GCTACCCCTT ACTGGATGGC TGTAGAAAAA ATAGGCAGAA ATGGCAGTCC 
TTAGCTGAAC AACAAGAAAA AAATCTGATT AATGGAGAAA GCAATCAAGC CAAACGGAAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013807106.1
CDS	107..2569
Translation	MVNAWFAERVHTIPVCKEGTKTQSESCSCHQPACAETTNAGTPARKISASEFDRPLRPIFVKDSVGAVSFLSDSEKKEQMPLQSPRIGTSAGDQCSRLLELVKDISSHLDVTALCHKIFLHIHELIAADRYSLFLVCEDSSNEKFLVSRLFDVAEGSTLEEASNNCIRLEWNKGIVGHVAAIGQPLNIKNAYEDPRFNAEVDQITGYKTQSILCMPIKNHREEVVGVAQAINKKSGIGGTFTEQDEKDFAAYLAFCGIVLHNAQLYETSLLENRRNQVLLDLASLIFEEQQSLEVILKKIAATIISFMQVQRCTIFIVDEDCTDSFSSVFHMESEELEDSAENLKRDYDTNKINYMYAEYVKNTMEPLNIPDVCKDRRFPWTNDNAENVSQNIKSLLCTPIKNGKKNKVIGVCQLVNKMEENSGKIKAFNRNDEQFLEAFVIFCGLGIQNTQMYEAVERAMAKQMVTLEVLSYHASAAEEETRELQVTAAAVVPSAQSLNLTDFNFSDFELSDFETTLCTIRMFTDLNLVQNFQMKHEVLCRWILSVKKNYRKNVAYHNWRHAFNTAQCMFAALKSGKIQSKLTDLETLALLIAALSHDLDHRGVNNSYIQRSEHPLAQLYCHSIMEHHHFDQCLMILNSPGNQILSSLSIEEYKATLKMIKQAILATDLALYIKRRGEFFELLRKKQFNWEDPMQKELFLAMLMTACDLSAITKPWPVQQRIAELVATEFFDQGDKERKELNIEPTDLMNREKKNKIPSMQVGFIDAVCLQLYEALTHVSEACYPLLDGCRKNRQKWQSLAEQQEKNLINGESNQAKRN

Target ORF information:

RefSeq Version	XM_013951652.1
Organism	Apteryx australis mantelli
Definition	Apteryx australis mantelli phosphodiesterase 5A, cGMP-specific (PDE5A), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013951652.1

ORF Insert Sequence:

ATGGTTAATG CATGGTTTGC TGAGAGAGTT CACACCATCC CAGTCTGCAA GGAGGGAACC 
AAGACCCAGA GTGAATCCTG CTCTTGTCAC CAGCCTGCCT GTGCTGAGAC CACCAACGCT 
GGGACTCCAG CGAGGAAAAT CTCTGCTTCT GAATTTGACA GACCCTTAAG GCCCATTTTT 
GTCAAGGACT CAGTAGGAGC AGTGAGCTTC CTCTCTGACT CTGAAAAGAA GGAACAGATG 
CCTCTGCAAT CTCCACGGAT TGGGACCAGC GCAGGGGATC AGTGCTCAAG ACTCTTAGAA 
CTTGTGAAAG ATATTTCTAG TCATTTGGAT GTCACAGCGC TTTGCCACAA AATTTTCCTA 
CATATCCATG AGCTTATAGC AGCTGATCGT TATTCCCTGT TCTTGGTCTG TGAGGACAGC 
TCTAACGAGA AGTTTCTTGT AAGCAGGCTG TTTGATGTGG CAGAAGGCTC CACCCTGGAA 
GAAGCTTCAA ACAACTGCAT TCGCCTGGAG TGGAACAAGG GCATTGTGGG TCACGTAGCA 
GCTATAGGCC AGCCTCTGAA CATCAAGAAC GCTTATGAGG ATCCAAGATT CAATGCAGAA 
GTTGACCAGA TCACCGGCTA TAAGACTCAG AGTATTCTGT GTATGCCAAT AAAAAATCAC 
AGGGAAGAGG TTGTTGGTGT GGCTCAAGCT ATCAATAAAA AATCTGGAAT TGGTGGGACT 
TTCACTGAAC AAGATGAAAA GGATTTTGCT GCTTATCTGG CATTTTGTGG CATTGTTCTT 
CACAACGCTC AGTTGTATGA GACATCTTTG CTTGAAAACA GACGTAACCA GGTGCTTCTT 
GATCTTGCCA GCCTGATTTT TGAAGAGCAG CAGTCTTTGG AAGTAATTTT GAAGAAGATA 
GCTGCCACTA TAATATCCTT CATGCAAGTG CAGAGGTGTA CCATTTTTAT AGTGGATGAA 
GATTGTACTG ATTCATTTTC TAGTGTGTTT CACATGGAAT CTGAGGAATT AGAAGATTCA 
GCTGAAAACC TGAAGAGGGA CTATGATACA AACAAAATCA ATTATATGTA TGCTGAATAT 
GTCAAGAATA CAATGGAGCC CCTCAACATC CCAGATGTCT GCAAAGATAG AAGATTTCCC 
TGGACAAATG ACAATGCAGA AAATGTAAGC CAAAACATAA AGAGTTTGCT GTGTACACCA 
ATCAAAAACG GGAAAAAGAA TAAGGTGATA GGGGTTTGTC AGCTGGTGAA TAAGATGGAA 
GAAAACTCAG GCAAAATCAA GGCTTTCAAC AGAAATGATG AACAGTTCCT GGAAGCATTT 
GTCATCTTTT GTGGCTTGGG AATCCAGAAT ACACAGATGT ATGAAGCTGT AGAAAGAGCA 
ATGGCCAAGC AGATGGTGAC ATTAGAGGTT CTCTCGTACC ATGCTTCTGC TGCTGAGGAG 
GAAACGAGGG AGCTGCAGGT AACAGCGGCT GCTGTAGTAC CATCTGCACA ATCTCTCAAC 
CTAACTGACT TCAACTTCAG TGATTTTGAG CTATCAGATT TTGAAACAAC ACTGTGTACA 
ATTCGAATGT TCACAGACCT CAACCTGGTG CAAAATTTCC AAATGAAGCA TGAGGTTCTC 
TGCAGATGGA TTTTAAGTGT AAAGAAAAAT TATCGGAAAA ATGTTGCTTA TCACAATTGG 
AGACATGCCT TTAATACGGC CCAGTGCATG TTTGCTGCTT TAAAATCTGG TAAAATTCAG 
AGCAAACTGA CTGACTTAGA AACACTTGCT TTGCTGATAG CAGCTCTAAG CCATGATTTG 
GATCACAGAG GAGTGAACAA CTCTTACATA CAAAGAAGTG AACATCCACT GGCTCAACTG 
TATTGCCACT CAATCATGGA GCATCACCAT TTCGATCAAT GCCTTATGAT CCTGAATAGT 
CCAGGAAATC AGATTCTCAG CAGCCTTTCC ATTGAAGAAT ACAAGGCCAC ACTGAAAATG 
ATAAAACAAG CCATTCTTGC CACAGACCTA GCACTATATA TAAAGAGGAG GGGAGAGTTT 
TTTGAACTTC TAAGGAAGAA GCAATTTAAT TGGGAAGATC CTATGCAGAA GGAGCTATTT 
TTAGCAATGC TGATGACTGC TTGTGATTTA TCAGCCATAA CCAAACCATG GCCAGTTCAG 
CAACGGATTG CTGAGCTTGT AGCTACAGAG TTCTTTGACC AAGGAGATAA AGAGCGGAAA 
GAACTCAACA TAGAACCAAC AGATCTAATG AACAGAGAGA AGAAAAATAA AATTCCCAGC 
ATGCAGGTTG GATTCATAGA TGCTGTTTGT TTACAGCTGT ATGAGGCCCT AACACACGTA 
TCAGAGGCCT GCTACCCCTT ACTGGATGGC TGTAGAAAAA ATAGGCAGAA ATGGCAGTCC 
TTAGCTGAAC AACAAGAAAA AAATCTGATT AATGGAGAAA GCAATCAAGC CAAACGGAAC 
TGA