mkrn1 cDNA ORF clone, Amphiprion ocellaris(clown anemonefish)

Gene
Clones
gRNAs

Summary

Gene Symbol	mkrn1
Entrez Gene ID	111583632
Full Name	makorin ring finger protein 1
Gene Type	protein-coding
Organism	Amphiprion ocellaris(clown anemonefish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_023292813.1	XP_023148581.1	E3 ubiquitin-protein ligase makorin-1

Pathways from BioSystems
Gene Ontology

The following mkrn1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the mkrn1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAm95516	XM_023292813.1 Latest version!	Amphiprion ocellaris makorin ring finger protein 1 (mkrn1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

mkrn1 ( XM_023292813.1 ) cDNA ORF clone, Amphiprion ocellaris(clown anemonefish) -> XP_023148581.1 Amphiprion ocellaris makorin ring finger protein 1 (mkrn1), mRNA.

Price & Availability↑

CloneID	OAm95516
Clone ID Related Accession (Same CDS sequence)	XM_023292813.1
Accession Version	XM_023292813.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1293bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-12
Organism	Amphiprion ocellaris(clown anemonefish)
Product	E3 ubiquitin-protein ligase makorin-1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_019373956.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Amphiprion ocellaris Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGAGG CAGCGGCAGC GTCTACGGCG GCTTCACCAG TAACAGGAGG TTGGACTAAA 
CACGTAACCT GCAGGTATTT CATGCATGGT CTTTGCAAAG AAGGAGACAA CTGTCGATAT 
TCTCACGATC TGACCAGCAG CAAACCAGCA GCCATGATCT GCAAGTTCTT TCAGAACGGA 
AACTGTGTGT TTGGAGACCA TTGCAGGTTT GAACACTGTA AATCAACAAA GAACGAGGAA 
CTGCCAACCT CGCAGATGCT GCCTCTGCCC TCCGCCTCGC TGGCTGGCCC CTCAGACCCT 
GAACCCAGTG GACCAACACC TGGGTCAGGC GTACAAGACT GGGTCAATGC TGCTGAGTTT 
GTTCCCGGAC AGCCGTACTG TGGACGGGCT GAGCCAGTGA AGGAGAACTC CCTCCCACTC 
ATTGAAGAGT TTGACAGTGA TGCAGTGCTG GACAACAAAG AGTTGAGGAA GCAGCTCTGT 
CCATATGCTG CAGTTGGAGA GTGTCGCTAC GGAATCAACT GTGCCTATCT CCACGGTGAT 
GTGTGCGACA TGTGTGGCCT CCAGGTGCTC CACCCCACCG ACAACACCCA GCGCTCAGAC 
CACACTAAGG CATGCATTGA AGCCCATGAG AAAGACATGG AGATTTCATT TGCCATCCAA 
CGCAGCAAGG ATATGATGTG TGGTGTGTGT ATGGAGGTGG TATTTGAGAA GGCCAACCCA 
AGTGAGCGCC GTTTTGGCAT CCTCTCCAAC TGCAATCATT GCTACTGTCT AAAGTGCATT 
CGCAAGTGGA GGAGTGCCAA GCAGTTTGAG AGCAAAATCA TCAAGTCCTG TCCAGAGTGC 
CGAATCACAT CCAACTTTGT CATCCCAAGC GAGTACTGGG TGGAAGATAA GGATGACAAA 
CAGAAACTCA TCCAGAAATA CAAGGATGGC ATGGGGAGTA AAGCATGTCG ATACTTTGAT 
GAGGGTCGTG GAACCTGCCC TTTCGGCTCA AACTGCTTTT ACAAGCACGC CTTTCCTGAT 
GGACGACTGG AAGAAGCTCA ACCTCAGCGA AGGCAAAATG GATCCAACAG CAGGAACCGG 
AACTCGAGGC GAACACCACT GTGGGACATC TTTGACGAGC AGGAAAGCAC CGACTCCTTT 
GACAACGAGG ATGAGGAGAT GGTGACGTTT GAGCTGAGCG AGATGCTTCT CATGCTGCTC 
GCCGCAGGAA CCGATGATGA GGTAACAGAT TCTGAGGATG AATGGGACTT GTTTCATGAG 
GAGCTGGACG ATTTCTACGA GATTTACCTA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023148581.1
CDS	102..1394
Translation	MAEAAAASTAASPVTGGWTKHVTCRYFMHGLCKEGDNCRYSHDLTSSKPAAMICKFFQNGNCVFGDHCRFEHCKSTKNEELPTSQMLPLPSASLAGPSDPEPSGPTPGSGVQDWVNAAEFVPGQPYCGRAEPVKENSLPLIEEFDSDAVLDNKELRKQLCPYAAVGECRYGINCAYLHGDVCDMCGLQVLHPTDNTQRSDHTKACIEAHEKDMEISFAIQRSKDMMCGVCMEVVFEKANPSERRFGILSNCNHCYCLKCIRKWRSAKQFESKIIKSCPECRITSNFVIPSEYWVEDKDDKQKLIQKYKDGMGSKACRYFDEGRGTCPFGSNCFYKHAFPDGRLEEAQPQRRQNGSNSRNRNSRRTPLWDIFDEQESTDSFDNEDEEMVTFELSEMLLMLLAAGTDDEVTDSEDEWDLFHEELDDFYEIYL

Target ORF information:

RefSeq Version	XM_023292813.1
Organism	Amphiprion ocellaris(clown anemonefish)
Definition	Amphiprion ocellaris makorin ring finger protein 1 (mkrn1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023292813.1

ORF Insert Sequence:

ATGGCGGAGG CAGCGGCAGC GTCTACGGCG GCTTCACCAG TAACAGGAGG TTGGACTAAA 
CACGTAACCT GCAGGTATTT CATGCATGGT CTTTGCAAAG AAGGAGACAA CTGTCGATAT 
TCTCACGATC TGACCAGCAG CAAACCAGCA GCCATGATCT GCAAGTTCTT TCAGAACGGA 
AACTGTGTGT TTGGAGACCA TTGCAGGTTT GAACACTGTA AATCAACAAA GAACGAGGAA 
CTGCCAACCT CGCAGATGCT GCCTCTGCCC TCCGCCTCGC TGGCTGGCCC CTCAGACCCT 
GAACCCAGTG GACCAACACC TGGGTCAGGC GTACAAGACT GGGTCAATGC TGCTGAGTTT 
GTTCCCGGAC AGCCGTACTG TGGACGGGCT GAGCCAGTGA AGGAGAACTC CCTCCCACTC 
ATTGAAGAGT TTGACAGTGA TGCAGTGCTG GACAACAAAG AGTTGAGGAA GCAGCTCTGT 
CCATATGCTG CAGTTGGAGA GTGTCGCTAC GGAATCAACT GTGCCTATCT CCACGGTGAT 
GTGTGCGACA TGTGTGGCCT CCAGGTGCTC CACCCCACCG ACAACACCCA GCGCTCAGAC 
CACACTAAGG CATGCATTGA AGCCCATGAG AAAGACATGG AGATTTCATT TGCCATCCAA 
CGCAGCAAGG ATATGATGTG TGGTGTGTGT ATGGAGGTGG TATTTGAGAA GGCCAACCCA 
AGTGAGCGCC GTTTTGGCAT CCTCTCCAAC TGCAATCATT GCTACTGTCT AAAGTGCATT 
CGCAAGTGGA GGAGTGCCAA GCAGTTTGAG AGCAAAATCA TCAAGTCCTG TCCAGAGTGC 
CGAATCACAT CCAACTTTGT CATCCCAAGC GAGTACTGGG TGGAAGATAA GGATGACAAA 
CAGAAACTCA TCCAGAAATA CAAGGATGGC ATGGGGAGTA AAGCATGTCG ATACTTTGAT 
GAGGGTCGTG GAACCTGCCC TTTCGGCTCA AACTGCTTTT ACAAGCACGC CTTTCCTGAT 
GGACGACTGG AAGAAGCTCA ACCTCAGCGA AGGCAAAATG GATCCAACAG CAGGAACCGG 
AACTCGAGGC GAACACCACT GTGGGACATC TTTGACGAGC AGGAAAGCAC CGACTCCTTT 
GACAACGAGG ATGAGGAGAT GGTGACGTTT GAGCTGAGCG AGATGCTTCT CATGCTGCTC 
GCCGCAGGAA CCGATGATGA GGTAACAGAT TCTGAGGATG AATGGGACTT GTTTCATGAG 
GAGCTGGACG ATTTCTACGA GATTTACCTA TAG