LOC111564892 cDNA ORF clone, Amphiprion ocellaris(clown anemonefish)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC111564892
Entrez Gene ID	111564892
Full Name	proline-rich transmembrane protein 4-like
Gene Type	protein-coding
Organism	Amphiprion ocellaris(clown anemonefish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_023264757.1	XP_023120525.1	proline-rich transmembrane protein 4-like
XM_023264756.1	XP_023120524.1	proline-rich transmembrane protein 4-like

Pathways from BioSystems
Gene Ontology

The following LOC111564892 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC111564892 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAm103934	XM_023264756.1 Latest version!	Amphiprion ocellaris proline-rich transmembrane protein 4-like (LOC111564892), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OAm103934	XM_023264757.1 Latest version!	Amphiprion ocellaris proline-rich transmembrane protein 4-like (LOC111564892), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC111564892 ( XM_023264757.1 ) cDNA ORF clone, Amphiprion ocellaris(clown anemonefish) -> XP_023120525.1 Amphiprion ocellaris proline-rich transmembrane protein 4-like (LOC111564892), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OAm103934
Clone ID Related Accession (Same CDS sequence)	XM_023264757.1 , XM_023264756.1
Accession Version	XM_023264757.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2931bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-12
Organism	Amphiprion ocellaris(clown anemonefish)
Product	proline-rich transmembrane protein 4-like
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_019376188.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Amphiprion ocellaris Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTTTGT GGTCCAGTTT GCAGAGCCTC ACCATGGTCT CTCTGTGGAT TATTTATCTT 
CTCCTCCCCC TTTCCTTGCC TCACTCACTT CATGTCATAG CGTTAACTGG AGAAAATGTC 
AAAGACGCGC AACAGCCAGA CATTGACACA ACAGTGCAGT TTCTCACACA GCTCTCACAT 
GGACCTCATG GATCGGGTAA TCCCATGTTT GGACCTGACA ATGCAGACCT CATGCGTCCA 
CCTTTGAGTT TGCCCTTATC ATCGTCTGAG GAAACAGATA GGCAAGGTGT GATTGGTGAA 
TACAGTGATG TACAGGAGAG CACAGAGACC TCTTTGTTGT ATCCTAACGA GAAGGAAGTC 
ATCATATCCA GTCCCACAGG GAGTGCTGCT GACGATGTTA CTCTCAAGGA GAGAATACAA 
TCAACAAGGT CTTCATCAGG AAGCATGCCA CTTAACCCCA CACAGGAGCA GACATTAAAG 
TATCATTCAG CTGAGAGCAA CACTGCATCT GTTTTGCAGC CTACAAAGAC TGGAGACACT 
CAGCCAACAT TCCCAGCCTT CAAAACAGAG ATGACTGAAA GCCAGCTACC TTTTCTTCTG 
TCTGGCTCAC TCCCATCACA CACACCAGAG CAGAACTCAA CTTCATTGTT GTCTGCTGGA 
CCTGGGCCTG GCACTGGACA TCCTACTCCA CCACTAGCCA CTGACAGCTG GGGATGGACT 
ACAGGCATTT CTGTTAGCAC ACAGGGAACA GGAGAGAGAT TAATTGATTT TACAGATCGT 
ATTTTACACA GAGGAACTGT CAGCACAGAG GCAGACAGAG ATGGCTCCAA GAATGATGAC 
CTGCATCTCT CTAAGGCCGG TGGCACCGTC TTGCAAGATG GGGACATGGA CACATCTGTC 
ACACCCTGCA AACCATCAAA TCAGTCCTGG ACTCCCACCT ACCCTGATGA TTTGACTCCC 
AACGAGCCAC TGCACCCGTC CCTGGCTGTC AGCCCTGCCC TTTTGGTCCC CCTGTATTCA 
GACTGGAACT CTGCCCTCGC TACGTGGGGG TTTGCGTGGG AAGCACACAT CTATGGCCTG 
GGGTCCATCT TCACTGCATT TGGCCTAATC TCCGTGGTCT GCCTGTTAGG CCTGCCCCTG 
CGCTGTCCCC CAGGAAGCCC CTACTTCACT TTGCTACACT TGTTCATTCT CGCATTTGCA 
GGGATCCAAG CTTTCTGTTT GCTCTTTGAT GCGTACAGTT ACCAAGATCG TCTTCCCCCT 
GTGGTCTCTC TGCTGCTCTC TGAACTGCCA TTGCCCTGTT TGATCTCGGC CTTCTCCCTG 
GCCTTCCTCC TTCTGTCCTT GCGCTCACGC ATGCATCTTT CACTCCCACT TGCTATTTCT 
ACCTCATTCT CAGCCTTGCC CAAACCTTGC CTATTACTAA GTATGTCCTT GGTACATTTT 
GTAATCTCTC TAGGCTGTGT TGGCATTCTC CAGCTCTTCC ACAGCCTCCC TACTGTGATC 
TTGTTATTAC CTCAGGGTGT GTTTGTATGT CTCACCCTAT TTCTCTCATG CTTCTACTTC 
ATCTTCTACT GCTTAACACA AGTCAACACT AAACGCATTT ATAGATTGAA TGACAACGGA 
GAGAGTGGAG GATCTCCTGA AGTGATGCGA CCTGCAGTTT GCCCTTTTGC CAAACTAGAG 
GACTGGGATA GGGCTGCAGG AGCTGCAATA GGAACTTCCT TGTGCTTGTT AGGGTGTGGA 
GGCTTTCAGC TTTATGGAAT CCTACATGCT CTTGGTTTTG GAGGGGTTGA TGGCTATGGG 
TTTCGGCCCT GGCCCTGGTG GGGTTACCAG GTAGGCTGCA GAATCTGTGA GATTGGGGTG 
TGTCTAGGTT TGTCTCTCAT TGGCACACAC CCTCTATTCT GTCACAGCAA CTCCTCTATC 
AAGACCCTAA CTCAGCCCCG GCCAGGAACT TGGTCCCGGC TATCTTGCAG CTCCCCTTCA 
CGAGGGCTCA CCTTGCCCTC ACAGGATGGT GCAAATTCTC CTGTTCTCTC CTCTCACCAG 
TCCTGGTCCC AGGGGAAGCA GGAAAAGCTG GTGGTCTGTG ATGTTGTCAG CAAAGGGCAG 
TCAGAGGCTC TTCCTCTTTG CTCAATAGCA GATCCTCCTG GAAATGGACT AAACTGTGTC 
CACAAGCCCA GTCAAACCCA GAATGTACTG CCTCTACCTA CACCCCCATG CCCACCACGT 
AAACCTGAAA ATTCAGCCAA GTCTCAGCTA TCTTCACTGG TTAATTTAGG TATAGAGACT 
GACTCTACTG TGGATCTGCG CCCTCCATCT CCCATAGACT TATCCCGCAG TATTGACCAA 
GCTCTGTTAA GTGAATCTCT ATTCTCTCAC AGTATTTTTG GTTTGCCAAG GCTGCTTCAC 
ACTTCGTCAA GCCTTTCCTT GAGCTCTCCT AGTCAAAGCA CATCAAAGCA GGGGCCCAGC 
TCTGTGGAAA ATAGCCTTTA CCGAACCTCT TCCTGTGGAG ATGTGGATCA AGAGAAAGCC 
CTTTCCAGCT CCAAACCCTC TCAAGCACAT GGCTGTTTGA CATCTCACAG CAACACACCG 
ACATCACCAG AGCGATGGGA TTGGAAGGGC AGTGTTACCG GTTCGACCCA GGGTCTATGC 
AGCCACCCCA AAGAAACAGG AAAGCTGCGC TCACATTCCT GGGCAAACAG AGGGCAAAAC 
TTTACTCAGA GCAGCCTCCC ACGAGCAATC CCCCACCTGT CGTACCACAG GAGGTACCGA 
ACCCTGAGCT TAGCCTCCCA GGACAGCCAA GGGTCTGGCC GATTGGCAGG GACAAAACAC 
CTGAGTGAAA GCAAACAGCT GGAATGGGAT CTGGCTGTGC AAGCAGAGTT TGTCAATGTT 
TGCAGACAAA TTGATGCCCT GAGTGTTTGC AGTGACACTA TTGAATTGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023120525.1
CDS	207..3137
Translation	MTLWSSLQSLTMVSLWIIYLLLPLSLPHSLHVIALTGENVKDAQQPDIDTTVQFLTQLSHGPHGSGNPMFGPDNADLMRPPLSLPLSSSEETDRQGVIGEYSDVQESTETSLLYPNEKEVIISSPTGSAADDVTLKERIQSTRSSSGSMPLNPTQEQTLKYHSAESNTASVLQPTKTGDTQPTFPAFKTEMTESQLPFLLSGSLPSHTPEQNSTSLLSAGPGPGTGHPTPPLATDSWGWTTGISVSTQGTGERLIDFTDRILHRGTVSTEADRDGSKNDDLHLSKAGGTVLQDGDMDTSVTPCKPSNQSWTPTYPDDLTPNEPLHPSLAVSPALLVPLYSDWNSALATWGFAWEAHIYGLGSIFTAFGLISVVCLLGLPLRCPPGSPYFTLLHLFILAFAGIQAFCLLFDAYSYQDRLPPVVSLLLSELPLPCLISAFSLAFLLLSLRSRMHLSLPLAISTSFSALPKPCLLLSMSLVHFVISLGCVGILQLFHSLPTVILLLPQGVFVCLTLFLSCFYFIFYCLTQVNTKRIYRLNDNGESGGSPEVMRPAVCPFAKLEDWDRAAGAAIGTSLCLLGCGGFQLYGILHALGFGGVDGYGFRPWPWWGYQVGCRICEIGVCLGLSLIGTHPLFCHSNSSIKTLTQPRPGTWSRLSCSSPSRGLTLPSQDGANSPVLSSHQSWSQGKQEKLVVCDVVSKGQSEALPLCSIADPPGNGLNCVHKPSQTQNVLPLPTPPCPPRKPENSAKSQLSSLVNLGIETDSTVDLRPPSPIDLSRSIDQALLSESLFSHSIFGLPRLLHTSSSLSLSSPSQSTSKQGPSSVENSLYRTSSCGDVDQEKALSSSKPSQAHGCLTSHSNTPTSPERWDWKGSVTGSTQGLCSHPKETGKLRSHSWANRGQNFTQSSLPRAIPHLSYHRRYRTLSLASQDSQGSGRLAGTKHLSESKQLEWDLAVQAEFVNVCRQIDALSVCSDTIEL

Target ORF information:

RefSeq Version	XM_023264757.1
Organism	Amphiprion ocellaris(clown anemonefish)
Definition	Amphiprion ocellaris proline-rich transmembrane protein 4-like (LOC111564892), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023264757.1

ORF Insert Sequence:

ATGACTTTGT GGTCCAGTTT GCAGAGCCTC ACCATGGTCT CTCTGTGGAT TATTTATCTT 
CTCCTCCCCC TTTCCTTGCC TCACTCACTT CATGTCATAG CGTTAACTGG AGAAAATGTC 
AAAGACGCGC AACAGCCAGA CATTGACACA ACAGTGCAGT TTCTCACACA GCTCTCACAT 
GGACCTCATG GATCGGGTAA TCCCATGTTT GGACCTGACA ATGCAGACCT CATGCGTCCA 
CCTTTGAGTT TGCCCTTATC ATCGTCTGAG GAAACAGATA GGCAAGGTGT GATTGGTGAA 
TACAGTGATG TACAGGAGAG CACAGAGACC TCTTTGTTGT ATCCTAACGA GAAGGAAGTC 
ATCATATCCA GTCCCACAGG GAGTGCTGCT GACGATGTTA CTCTCAAGGA GAGAATACAA 
TCAACAAGGT CTTCATCAGG AAGCATGCCA CTTAACCCCA CACAGGAGCA GACATTAAAG 
TATCATTCAG CTGAGAGCAA CACTGCATCT GTTTTGCAGC CTACAAAGAC TGGAGACACT 
CAGCCAACAT TCCCAGCCTT CAAAACAGAG ATGACTGAAA GCCAGCTACC TTTTCTTCTG 
TCTGGCTCAC TCCCATCACA CACACCAGAG CAGAACTCAA CTTCATTGTT GTCTGCTGGA 
CCTGGGCCTG GCACTGGACA TCCTACTCCA CCACTAGCCA CTGACAGCTG GGGATGGACT 
ACAGGCATTT CTGTTAGCAC ACAGGGAACA GGAGAGAGAT TAATTGATTT TACAGATCGT 
ATTTTACACA GAGGAACTGT CAGCACAGAG GCAGACAGAG ATGGCTCCAA GAATGATGAC 
CTGCATCTCT CTAAGGCCGG TGGCACCGTC TTGCAAGATG GGGACATGGA CACATCTGTC 
ACACCCTGCA AACCATCAAA TCAGTCCTGG ACTCCCACCT ACCCTGATGA TTTGACTCCC 
AACGAGCCAC TGCACCCGTC CCTGGCTGTC AGCCCTGCCC TTTTGGTCCC CCTGTATTCA 
GACTGGAACT CTGCCCTCGC TACGTGGGGG TTTGCGTGGG AAGCACACAT CTATGGCCTG 
GGGTCCATCT TCACTGCATT TGGCCTAATC TCCGTGGTCT GCCTGTTAGG CCTGCCCCTG 
CGCTGTCCCC CAGGAAGCCC CTACTTCACT TTGCTACACT TGTTCATTCT CGCATTTGCA 
GGGATCCAAG CTTTCTGTTT GCTCTTTGAT GCGTACAGTT ACCAAGATCG TCTTCCCCCT 
GTGGTCTCTC TGCTGCTCTC TGAACTGCCA TTGCCCTGTT TGATCTCGGC CTTCTCCCTG 
GCCTTCCTCC TTCTGTCCTT GCGCTCACGC ATGCATCTTT CACTCCCACT TGCTATTTCT 
ACCTCATTCT CAGCCTTGCC CAAACCTTGC CTATTACTAA GTATGTCCTT GGTACATTTT 
GTAATCTCTC TAGGCTGTGT TGGCATTCTC CAGCTCTTCC ACAGCCTCCC TACTGTGATC 
TTGTTATTAC CTCAGGGTGT GTTTGTATGT CTCACCCTAT TTCTCTCATG CTTCTACTTC 
ATCTTCTACT GCTTAACACA AGTCAACACT AAACGCATTT ATAGATTGAA TGACAACGGA 
GAGAGTGGAG GATCTCCTGA AGTGATGCGA CCTGCAGTTT GCCCTTTTGC CAAACTAGAG 
GACTGGGATA GGGCTGCAGG AGCTGCAATA GGAACTTCCT TGTGCTTGTT AGGGTGTGGA 
GGCTTTCAGC TTTATGGAAT CCTACATGCT CTTGGTTTTG GAGGGGTTGA TGGCTATGGG 
TTTCGGCCCT GGCCCTGGTG GGGTTACCAG GTAGGCTGCA GAATCTGTGA GATTGGGGTG 
TGTCTAGGTT TGTCTCTCAT TGGCACACAC CCTCTATTCT GTCACAGCAA CTCCTCTATC 
AAGACCCTAA CTCAGCCCCG GCCAGGAACT TGGTCCCGGC TATCTTGCAG CTCCCCTTCA 
CGAGGGCTCA CCTTGCCCTC ACAGGATGGT GCAAATTCTC CTGTTCTCTC CTCTCACCAG 
TCCTGGTCCC AGGGGAAGCA GGAAAAGCTG GTGGTCTGTG ATGTTGTCAG CAAAGGGCAG 
TCAGAGGCTC TTCCTCTTTG CTCAATAGCA GATCCTCCTG GAAATGGACT AAACTGTGTC 
CACAAGCCCA GTCAAACCCA GAATGTACTG CCTCTACCTA CACCCCCATG CCCACCACGT 
AAACCTGAAA ATTCAGCCAA GTCTCAGCTA TCTTCACTGG TTAATTTAGG TATAGAGACT 
GACTCTACTG TGGATCTGCG CCCTCCATCT CCCATAGACT TATCCCGCAG TATTGACCAA 
GCTCTGTTAA GTGAATCTCT ATTCTCTCAC AGTATTTTTG GTTTGCCAAG GCTGCTTCAC 
ACTTCGTCAA GCCTTTCCTT GAGCTCTCCT AGTCAAAGCA CATCAAAGCA GGGGCCCAGC 
TCTGTGGAAA ATAGCCTTTA CCGAACCTCT TCCTGTGGAG ATGTGGATCA AGAGAAAGCC 
CTTTCCAGCT CCAAACCCTC TCAAGCACAT GGCTGTTTGA CATCTCACAG CAACACACCG 
ACATCACCAG AGCGATGGGA TTGGAAGGGC AGTGTTACCG GTTCGACCCA GGGTCTATGC 
AGCCACCCCA AAGAAACAGG AAAGCTGCGC TCACATTCCT GGGCAAACAG AGGGCAAAAC 
TTTACTCAGA GCAGCCTCCC ACGAGCAATC CCCCACCTGT CGTACCACAG GAGGTACCGA 
ACCCTGAGCT TAGCCTCCCA GGACAGCCAA GGGTCTGGCC GATTGGCAGG GACAAAACAC 
CTGAGTGAAA GCAAACAGCT GGAATGGGAT CTGGCTGTGC AAGCAGAGTT TGTCAATGTT 
TGCAGACAAA TTGATGCCCT GAGTGTTTGC AGTGACACTA TTGAATTGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC111564892 ( XM_023264756.1 ) cDNA ORF clone, Amphiprion ocellaris(clown anemonefish) -> XP_023120524.1 Amphiprion ocellaris proline-rich transmembrane protein 4-like (LOC111564892), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OAm103934
Clone ID Related Accession (Same CDS sequence)	XM_023264757.1 , XM_023264756.1
Accession Version	XM_023264756.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2931bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-12
Organism	Amphiprion ocellaris(clown anemonefish)
Product	proline-rich transmembrane protein 4-like
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_019376188.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Amphiprion ocellaris Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTTTGT GGTCCAGTTT GCAGAGCCTC ACCATGGTCT CTCTGTGGAT TATTTATCTT 
CTCCTCCCCC TTTCCTTGCC TCACTCACTT CATGTCATAG CGTTAACTGG AGAAAATGTC 
AAAGACGCGC AACAGCCAGA CATTGACACA ACAGTGCAGT TTCTCACACA GCTCTCACAT 
GGACCTCATG GATCGGGTAA TCCCATGTTT GGACCTGACA ATGCAGACCT CATGCGTCCA 
CCTTTGAGTT TGCCCTTATC ATCGTCTGAG GAAACAGATA GGCAAGGTGT GATTGGTGAA 
TACAGTGATG TACAGGAGAG CACAGAGACC TCTTTGTTGT ATCCTAACGA GAAGGAAGTC 
ATCATATCCA GTCCCACAGG GAGTGCTGCT GACGATGTTA CTCTCAAGGA GAGAATACAA 
TCAACAAGGT CTTCATCAGG AAGCATGCCA CTTAACCCCA CACAGGAGCA GACATTAAAG 
TATCATTCAG CTGAGAGCAA CACTGCATCT GTTTTGCAGC CTACAAAGAC TGGAGACACT 
CAGCCAACAT TCCCAGCCTT CAAAACAGAG ATGACTGAAA GCCAGCTACC TTTTCTTCTG 
TCTGGCTCAC TCCCATCACA CACACCAGAG CAGAACTCAA CTTCATTGTT GTCTGCTGGA 
CCTGGGCCTG GCACTGGACA TCCTACTCCA CCACTAGCCA CTGACAGCTG GGGATGGACT 
ACAGGCATTT CTGTTAGCAC ACAGGGAACA GGAGAGAGAT TAATTGATTT TACAGATCGT 
ATTTTACACA GAGGAACTGT CAGCACAGAG GCAGACAGAG ATGGCTCCAA GAATGATGAC 
CTGCATCTCT CTAAGGCCGG TGGCACCGTC TTGCAAGATG GGGACATGGA CACATCTGTC 
ACACCCTGCA AACCATCAAA TCAGTCCTGG ACTCCCACCT ACCCTGATGA TTTGACTCCC 
AACGAGCCAC TGCACCCGTC CCTGGCTGTC AGCCCTGCCC TTTTGGTCCC CCTGTATTCA 
GACTGGAACT CTGCCCTCGC TACGTGGGGG TTTGCGTGGG AAGCACACAT CTATGGCCTG 
GGGTCCATCT TCACTGCATT TGGCCTAATC TCCGTGGTCT GCCTGTTAGG CCTGCCCCTG 
CGCTGTCCCC CAGGAAGCCC CTACTTCACT TTGCTACACT TGTTCATTCT CGCATTTGCA 
GGGATCCAAG CTTTCTGTTT GCTCTTTGAT GCGTACAGTT ACCAAGATCG TCTTCCCCCT 
GTGGTCTCTC TGCTGCTCTC TGAACTGCCA TTGCCCTGTT TGATCTCGGC CTTCTCCCTG 
GCCTTCCTCC TTCTGTCCTT GCGCTCACGC ATGCATCTTT CACTCCCACT TGCTATTTCT 
ACCTCATTCT CAGCCTTGCC CAAACCTTGC CTATTACTAA GTATGTCCTT GGTACATTTT 
GTAATCTCTC TAGGCTGTGT TGGCATTCTC CAGCTCTTCC ACAGCCTCCC TACTGTGATC 
TTGTTATTAC CTCAGGGTGT GTTTGTATGT CTCACCCTAT TTCTCTCATG CTTCTACTTC 
ATCTTCTACT GCTTAACACA AGTCAACACT AAACGCATTT ATAGATTGAA TGACAACGGA 
GAGAGTGGAG GATCTCCTGA AGTGATGCGA CCTGCAGTTT GCCCTTTTGC CAAACTAGAG 
GACTGGGATA GGGCTGCAGG AGCTGCAATA GGAACTTCCT TGTGCTTGTT AGGGTGTGGA 
GGCTTTCAGC TTTATGGAAT CCTACATGCT CTTGGTTTTG GAGGGGTTGA TGGCTATGGG 
TTTCGGCCCT GGCCCTGGTG GGGTTACCAG GTAGGCTGCA GAATCTGTGA GATTGGGGTG 
TGTCTAGGTT TGTCTCTCAT TGGCACACAC CCTCTATTCT GTCACAGCAA CTCCTCTATC 
AAGACCCTAA CTCAGCCCCG GCCAGGAACT TGGTCCCGGC TATCTTGCAG CTCCCCTTCA 
CGAGGGCTCA CCTTGCCCTC ACAGGATGGT GCAAATTCTC CTGTTCTCTC CTCTCACCAG 
TCCTGGTCCC AGGGGAAGCA GGAAAAGCTG GTGGTCTGTG ATGTTGTCAG CAAAGGGCAG 
TCAGAGGCTC TTCCTCTTTG CTCAATAGCA GATCCTCCTG GAAATGGACT AAACTGTGTC 
CACAAGCCCA GTCAAACCCA GAATGTACTG CCTCTACCTA CACCCCCATG CCCACCACGT 
AAACCTGAAA ATTCAGCCAA GTCTCAGCTA TCTTCACTGG TTAATTTAGG TATAGAGACT 
GACTCTACTG TGGATCTGCG CCCTCCATCT CCCATAGACT TATCCCGCAG TATTGACCAA 
GCTCTGTTAA GTGAATCTCT ATTCTCTCAC AGTATTTTTG GTTTGCCAAG GCTGCTTCAC 
ACTTCGTCAA GCCTTTCCTT GAGCTCTCCT AGTCAAAGCA CATCAAAGCA GGGGCCCAGC 
TCTGTGGAAA ATAGCCTTTA CCGAACCTCT TCCTGTGGAG ATGTGGATCA AGAGAAAGCC 
CTTTCCAGCT CCAAACCCTC TCAAGCACAT GGCTGTTTGA CATCTCACAG CAACACACCG 
ACATCACCAG AGCGATGGGA TTGGAAGGGC AGTGTTACCG GTTCGACCCA GGGTCTATGC 
AGCCACCCCA AAGAAACAGG AAAGCTGCGC TCACATTCCT GGGCAAACAG AGGGCAAAAC 
TTTACTCAGA GCAGCCTCCC ACGAGCAATC CCCCACCTGT CGTACCACAG GAGGTACCGA 
ACCCTGAGCT TAGCCTCCCA GGACAGCCAA GGGTCTGGCC GATTGGCAGG GACAAAACAC 
CTGAGTGAAA GCAAACAGCT GGAATGGGAT CTGGCTGTGC AAGCAGAGTT TGTCAATGTT 
TGCAGACAAA TTGATGCCCT GAGTGTTTGC AGTGACACTA TTGAATTGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023120524.1
CDS	244..3174
Translation	MTLWSSLQSLTMVSLWIIYLLLPLSLPHSLHVIALTGENVKDAQQPDIDTTVQFLTQLSHGPHGSGNPMFGPDNADLMRPPLSLPLSSSEETDRQGVIGEYSDVQESTETSLLYPNEKEVIISSPTGSAADDVTLKERIQSTRSSSGSMPLNPTQEQTLKYHSAESNTASVLQPTKTGDTQPTFPAFKTEMTESQLPFLLSGSLPSHTPEQNSTSLLSAGPGPGTGHPTPPLATDSWGWTTGISVSTQGTGERLIDFTDRILHRGTVSTEADRDGSKNDDLHLSKAGGTVLQDGDMDTSVTPCKPSNQSWTPTYPDDLTPNEPLHPSLAVSPALLVPLYSDWNSALATWGFAWEAHIYGLGSIFTAFGLISVVCLLGLPLRCPPGSPYFTLLHLFILAFAGIQAFCLLFDAYSYQDRLPPVVSLLLSELPLPCLISAFSLAFLLLSLRSRMHLSLPLAISTSFSALPKPCLLLSMSLVHFVISLGCVGILQLFHSLPTVILLLPQGVFVCLTLFLSCFYFIFYCLTQVNTKRIYRLNDNGESGGSPEVMRPAVCPFAKLEDWDRAAGAAIGTSLCLLGCGGFQLYGILHALGFGGVDGYGFRPWPWWGYQVGCRICEIGVCLGLSLIGTHPLFCHSNSSIKTLTQPRPGTWSRLSCSSPSRGLTLPSQDGANSPVLSSHQSWSQGKQEKLVVCDVVSKGQSEALPLCSIADPPGNGLNCVHKPSQTQNVLPLPTPPCPPRKPENSAKSQLSSLVNLGIETDSTVDLRPPSPIDLSRSIDQALLSESLFSHSIFGLPRLLHTSSSLSLSSPSQSTSKQGPSSVENSLYRTSSCGDVDQEKALSSSKPSQAHGCLTSHSNTPTSPERWDWKGSVTGSTQGLCSHPKETGKLRSHSWANRGQNFTQSSLPRAIPHLSYHRRYRTLSLASQDSQGSGRLAGTKHLSESKQLEWDLAVQAEFVNVCRQIDALSVCSDTIEL

Target ORF information:

RefSeq Version	XM_023264756.1
Organism	Amphiprion ocellaris(clown anemonefish)
Definition	Amphiprion ocellaris proline-rich transmembrane protein 4-like (LOC111564892), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023264756.1

ORF Insert Sequence:

ATGACTTTGT GGTCCAGTTT GCAGAGCCTC ACCATGGTCT CTCTGTGGAT TATTTATCTT 
CTCCTCCCCC TTTCCTTGCC TCACTCACTT CATGTCATAG CGTTAACTGG AGAAAATGTC 
AAAGACGCGC AACAGCCAGA CATTGACACA ACAGTGCAGT TTCTCACACA GCTCTCACAT 
GGACCTCATG GATCGGGTAA TCCCATGTTT GGACCTGACA ATGCAGACCT CATGCGTCCA 
CCTTTGAGTT TGCCCTTATC ATCGTCTGAG GAAACAGATA GGCAAGGTGT GATTGGTGAA 
TACAGTGATG TACAGGAGAG CACAGAGACC TCTTTGTTGT ATCCTAACGA GAAGGAAGTC 
ATCATATCCA GTCCCACAGG GAGTGCTGCT GACGATGTTA CTCTCAAGGA GAGAATACAA 
TCAACAAGGT CTTCATCAGG AAGCATGCCA CTTAACCCCA CACAGGAGCA GACATTAAAG 
TATCATTCAG CTGAGAGCAA CACTGCATCT GTTTTGCAGC CTACAAAGAC TGGAGACACT 
CAGCCAACAT TCCCAGCCTT CAAAACAGAG ATGACTGAAA GCCAGCTACC TTTTCTTCTG 
TCTGGCTCAC TCCCATCACA CACACCAGAG CAGAACTCAA CTTCATTGTT GTCTGCTGGA 
CCTGGGCCTG GCACTGGACA TCCTACTCCA CCACTAGCCA CTGACAGCTG GGGATGGACT 
ACAGGCATTT CTGTTAGCAC ACAGGGAACA GGAGAGAGAT TAATTGATTT TACAGATCGT 
ATTTTACACA GAGGAACTGT CAGCACAGAG GCAGACAGAG ATGGCTCCAA GAATGATGAC 
CTGCATCTCT CTAAGGCCGG TGGCACCGTC TTGCAAGATG GGGACATGGA CACATCTGTC 
ACACCCTGCA AACCATCAAA TCAGTCCTGG ACTCCCACCT ACCCTGATGA TTTGACTCCC 
AACGAGCCAC TGCACCCGTC CCTGGCTGTC AGCCCTGCCC TTTTGGTCCC CCTGTATTCA 
GACTGGAACT CTGCCCTCGC TACGTGGGGG TTTGCGTGGG AAGCACACAT CTATGGCCTG 
GGGTCCATCT TCACTGCATT TGGCCTAATC TCCGTGGTCT GCCTGTTAGG CCTGCCCCTG 
CGCTGTCCCC CAGGAAGCCC CTACTTCACT TTGCTACACT TGTTCATTCT CGCATTTGCA 
GGGATCCAAG CTTTCTGTTT GCTCTTTGAT GCGTACAGTT ACCAAGATCG TCTTCCCCCT 
GTGGTCTCTC TGCTGCTCTC TGAACTGCCA TTGCCCTGTT TGATCTCGGC CTTCTCCCTG 
GCCTTCCTCC TTCTGTCCTT GCGCTCACGC ATGCATCTTT CACTCCCACT TGCTATTTCT 
ACCTCATTCT CAGCCTTGCC CAAACCTTGC CTATTACTAA GTATGTCCTT GGTACATTTT 
GTAATCTCTC TAGGCTGTGT TGGCATTCTC CAGCTCTTCC ACAGCCTCCC TACTGTGATC 
TTGTTATTAC CTCAGGGTGT GTTTGTATGT CTCACCCTAT TTCTCTCATG CTTCTACTTC 
ATCTTCTACT GCTTAACACA AGTCAACACT AAACGCATTT ATAGATTGAA TGACAACGGA 
GAGAGTGGAG GATCTCCTGA AGTGATGCGA CCTGCAGTTT GCCCTTTTGC CAAACTAGAG 
GACTGGGATA GGGCTGCAGG AGCTGCAATA GGAACTTCCT TGTGCTTGTT AGGGTGTGGA 
GGCTTTCAGC TTTATGGAAT CCTACATGCT CTTGGTTTTG GAGGGGTTGA TGGCTATGGG 
TTTCGGCCCT GGCCCTGGTG GGGTTACCAG GTAGGCTGCA GAATCTGTGA GATTGGGGTG 
TGTCTAGGTT TGTCTCTCAT TGGCACACAC CCTCTATTCT GTCACAGCAA CTCCTCTATC 
AAGACCCTAA CTCAGCCCCG GCCAGGAACT TGGTCCCGGC TATCTTGCAG CTCCCCTTCA 
CGAGGGCTCA CCTTGCCCTC ACAGGATGGT GCAAATTCTC CTGTTCTCTC CTCTCACCAG 
TCCTGGTCCC AGGGGAAGCA GGAAAAGCTG GTGGTCTGTG ATGTTGTCAG CAAAGGGCAG 
TCAGAGGCTC TTCCTCTTTG CTCAATAGCA GATCCTCCTG GAAATGGACT AAACTGTGTC 
CACAAGCCCA GTCAAACCCA GAATGTACTG CCTCTACCTA CACCCCCATG CCCACCACGT 
AAACCTGAAA ATTCAGCCAA GTCTCAGCTA TCTTCACTGG TTAATTTAGG TATAGAGACT 
GACTCTACTG TGGATCTGCG CCCTCCATCT CCCATAGACT TATCCCGCAG TATTGACCAA 
GCTCTGTTAA GTGAATCTCT ATTCTCTCAC AGTATTTTTG GTTTGCCAAG GCTGCTTCAC 
ACTTCGTCAA GCCTTTCCTT GAGCTCTCCT AGTCAAAGCA CATCAAAGCA GGGGCCCAGC 
TCTGTGGAAA ATAGCCTTTA CCGAACCTCT TCCTGTGGAG ATGTGGATCA AGAGAAAGCC 
CTTTCCAGCT CCAAACCCTC TCAAGCACAT GGCTGTTTGA CATCTCACAG CAACACACCG 
ACATCACCAG AGCGATGGGA TTGGAAGGGC AGTGTTACCG GTTCGACCCA GGGTCTATGC 
AGCCACCCCA AAGAAACAGG AAAGCTGCGC TCACATTCCT GGGCAAACAG AGGGCAAAAC 
TTTACTCAGA GCAGCCTCCC ACGAGCAATC CCCCACCTGT CGTACCACAG GAGGTACCGA 
ACCCTGAGCT TAGCCTCCCA GGACAGCCAA GGGTCTGGCC GATTGGCAGG GACAAAACAC 
CTGAGTGAAA GCAAACAGCT GGAATGGGAT CTGGCTGTGC AAGCAGAGTT TGTCAATGTT 
TGCAGACAAA TTGATGCCCT GAGTGTTTGC AGTGACACTA TTGAATTGTA G