LOC100574152 cDNA ORF clone, Acyrthosiphon pisum(pea aphid)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC100574152
Entrez Gene ID	100574152
Full Name	nicastrin
Gene Type	protein-coding
Organism	Acyrthosiphon pisum(pea aphid)

mRNA and Protein(s)

mRNA	Protein	Name
XM_029490207.1	XP_029346067.1	nicastrin
XM_003245001.3	XP_003245049.1	nicastrin

Pathways from BioSystems
Gene Ontology

The following LOC100574152 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC100574152 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAd10656	XM_003245001.3 Latest version!	Acyrthosiphon pisum nicastrin (LOC100574152), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OAd31983	XM_029490207.1 Latest version!	Acyrthosiphon pisum nicastrin (LOC100574152), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC100574152 ( XM_003245001.3 ) cDNA ORF clone, Acyrthosiphon pisum(pea aphid) -> XP_003245049.1 Acyrthosiphon pisum nicastrin (LOC100574152), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OAd10656
Clone ID Related Accession (Same CDS sequence)	XM_003245001.3
Accession Version	XM_003245001.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 312bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-05-16
Organism	Acyrthosiphon pisum(pea aphid)
Product	nicastrin
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003383849.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2016 this sequence version replaced XM_003245001.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Acyrthosiphon pisum Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTATAT GGTTAAGAGG AAATTATAAT GCGTGTTATT CAACAACATC TAATGTTACT 
GATGCAGTTA GCCCAGCATT TATTATACCT GATTATAATT TATCATCTCC AAAATATTCA 
ACATGGACTG AATCTGTATG GCATGAAGTG CATATTCGTA TGTTTCTTAG GCAATCGTTT 
AAACTACAAA TTATAATATT TGTACTTGGA GTTTTAATAT TTCTTTTTTC TTTCATCATA 
ATAAAAAAAA TGTATGATCA ATCTGGAATT CATTTTAATA ACCAAGAAGT CCATTCAGTA 
ACAAATTTTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003245049.1
CDS	45..356
Translation	MPIWLRGNYNACYSTTSNVTDAVSPAFIIPDYNLSSPKYSTWTESVWHEVHIRMFLRQSFKLQIIIFVLGVLIFLFSFIIIKKMYDQSGIHFNNQEVHSVTNF

Target ORF information:

RefSeq Version	XM_003245001.3
Organism	Acyrthosiphon pisum(pea aphid)
Definition	Acyrthosiphon pisum nicastrin (LOC100574152), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003245001.3

ORF Insert Sequence:

ATGCCTATAT GGTTAAGAGG AAATTATAAT GCGTGTTATT CAACAACATC TAATGTTACT 
GATGCAGTTA GCCCAGCATT TATTATACCT GATTATAATT TATCATCTCC AAAATATTCA 
ACATGGACTG AATCTGTATG GCATGAAGTG CATATTCGTA TGTTTCTTAG GCAATCGTTT 
AAACTACAAA TTATAATATT TGTACTTGGA GTTTTAATAT TTCTTTTTTC TTTCATCATA 
ATAAAAAAAA TGTATGATCA ATCTGGAATT CATTTTAATA ACCAAGAAGT CCATTCAGTA 
ACAAATTTTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100574152 ( XM_029490207.1 ) cDNA ORF clone, Acyrthosiphon pisum(pea aphid) -> XP_029346067.1 Acyrthosiphon pisum nicastrin (LOC100574152), mRNA.

Price & Availability↑

CloneID	OAd31983
Clone ID Related Accession (Same CDS sequence)	XM_029490207.1
Accession Version	XM_029490207.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 495bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-15
Organism	Acyrthosiphon pisum(pea aphid)
Product	nicastrin
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042495.1) and transcript sequence (HP427836.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Acyrthosiphon pisum Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 309 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGAATTGTG AAGTATTTCA ATCATTGACT TCACCACTGC ATTTACCTGA CAACCCCCCT 
AATTATTATA TCAGTATAGA TTTGATCACA AATACTATCA CTAGTCTTTC ACGCCTATTA 
TTTGCTTCTT TTGGAAGTAA AGTAATTAAT GAAGTTCAAA ATGAAGAAAA TTGTCTAAAC 
TATAGAACGA AACAGGGTTT TATGCCTATA TGGTTAAGAG GAAATTATAA TGCGTGTTAT 
TCAACAACAT CTAATGTTAC TGATGCAGTT AGCCCAGCAT TTATTATACC TGATTATAAT 
TTATCATCTC CAAAATATTC AACATGGACT GAATCTGTAT GGCATGAAGT GCATATTCGT 
ATGTTTCTTA GGCAATCGTT TAAACTACAA ATTATAATAT TTGTACTTGG AGTTTTAATA 
TTTCTTTTTT CTTTCATCAT AATAAAAAAA ATGTATGATC AATCTGGAAT TCATTTTAAT 
AACCAAGAGG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029346067.1
CDS	115..609
Translation	MNCEVFQSLTSPLHLPDNPPNYYISIDLITNTITSLSRLLFASFGSKVINEVQNEENCLNYRTKQGFMPIWLRGNYNACYSTTSNVTDAVSPAFIIPDYNLSSPKYSTWTESVWHEVHIRMFLRQSFKLQIIIFVLGVLIFLFSFIIIKKMYDQSGIHFNNQEE

Target ORF information:

RefSeq Version	XM_029490207.1
Organism	Acyrthosiphon pisum(pea aphid)
Definition	Acyrthosiphon pisum nicastrin (LOC100574152), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029490207.1

ORF Insert Sequence:

ATGAATTGTG AAGTATTTCA ATCATTGACT TCACCACTGC ATTTACCTGA CAACCCCCCT 
AATTATTATA TCAGTATAGA TTTGATCACA AATACTATCA CTAGTCTTTC ACGCCTATTA 
TTTGCTTCTT TTGGAAGTAA AGTAATTAAT GAAGTTCAAA ATGAAGAAAA TTGTCTAAAC 
TATAGAACGA AACAGGGTTT TATGCCTATA TGGTTAAGAG GAAATTATAA TGCGTGTTAT 
TCAACAACAT CTAATGTTAC TGATGCAGTT AGCCCAGCAT TTATTATACC TGATTATAAT 
TTATCATCTC CAAAATATTC AACATGGACT GAATCTGTAT GGCATGAAGT GCATATTCGT 
ATGTTTCTTA GGCAATCGTT TAAACTACAA ATTATAATAT TTGTACTTGG AGTTTTAATA 
TTTCTTTTTT CTTTCATCAT AATAAAAAAA ATGTATGATC AATCTGGAAT TCATTTTAAT 
AACCAAGAGG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.