ITGA5 cDNA ORF clone, Acinonyx jubatus(cheetah)

Gene
Clones
gRNAs

Summary

Gene Symbol	ITGA5
Entrez Gene ID	106989320
Full Name	integrin subunit alpha 5
Gene Type	protein-coding
Organism	Acinonyx jubatus(cheetah)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015087803.1	XP_014943289.1	integrin alpha-5
XM_027036357.1	XP_026892158.1	integrin alpha-5

Pathways from BioSystems
Gene Ontology

The following ITGA5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ITGA5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAc236391	XM_027036357.1 Latest version!	Acinonyx jubatus integrin subunit alpha 5 (ITGA5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OAc57592	XM_015087803.1 Latest version!	Acinonyx jubatus integrin alpha 5 (ITGA5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ITGA5 ( XM_027036357.1 ) cDNA ORF clone, Acinonyx jubatus(cheetah) -> XP_026892158.1 Acinonyx jubatus integrin subunit alpha 5 (ITGA5), mRNA.

Price & Availability↑

CloneID	OAc236391
Clone ID Related Accession (Same CDS sequence)	XM_027036357.1
Accession Version	XM_027036357.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3333bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-12
Organism	Acinonyx jubatus(cheetah)
Product	integrin alpha-5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_020834768.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Acinonyx jubatus Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCCCCC TCTCGCCCCC CCGGCCGGCG GGGGGAGGGC TCAGCCGGGA GTTTGGCAAA 
CTCCTCCCCG CGTTGAGTCA TTCGCCTCTG GGAGGTTTAG GAAGCGGCTC CGGGTCGGTG 
GCCCCAGGAC AGGGAAGAGC GGGCGCTATG GGGAGACGGG CACCAGGGTT CCCTCTCCAC 
GCCGTGCAAC TGCGCTGGGG CCCCCGGCGC CGACCCCCGC TGCTGCTGCT GCTGCCGCCG 
CTGCTGCTGC TGCTGCTGCC GCCGCCACCC AGGGTCGGGG GCTTCAATTT AGACGCGGAG 
GCCCCAGCTG TGCTCGCTGG GCCCCCGGGC TCCTTCTTCG GCTTCTCGGT GGAGTTTTAC 
CGGCCGGGGA CGGACGGGGT CAGCGTGCTG GTGGGAGCTC CCAAGGCCAA CACCAGCCAG 
CCGGGAGTGC TGCAGGGTGG TGCCGTCTAC CTCTGTCCCT GGGGCACCAG CCCGGTACAC 
TGCACCCCCA TTGAATTTGA CAGTAAAGGC TCTCGACTCC TGGAGTCATT ACTGCCCAGC 
CCAGAGGGAG AGGAGCCTGT GGAGTACAAG TCCTTGCAGT GGTTTGGAGC GACGGTTCGA 
GCCCACGGCT CCTCCATTTT GGCCTGTGCT CCTCTATATA GCTGGCGCAC AGAGAAGGAG 
CCGCTGAATG ATCCCGTGGG CACCTGCTAC CTCTCCACGG ACAATTTCAC CCGAATTCTG 
GAGTATGCAC CTTGCCGCTC AGATTTCAGC TGGGCCGCAG GACAGGGTTA CTGCCAAGGG 
GGCTTCAGTG CTGAGTTCAC CAAGACTGGG CGTGTGGTCC TGGGTGGACC AGGGAGCTAC 
TTCTGGCAAG GCCAGATCCT GTCGGCCACC CAAGAACAGA TTGCCGAATC CTATTACCCC 
GAGTACCTGA TCAACCTGGT TCAGGGGCAG CTGCAGACCC GCCAGGCCAG TTCCATCTAC 
GATGACAGCT ACCTGGGATA CTCTGTGGCT GTGGGTGAAT TCAGTGGTGA TGACACAGAA 
GACTTTGTCG CTGGCGTGCC CAAAGGGAAC CTCACTTATG GTTATGTCAC CATCCTCAAT 
GGCTCAGACA TCCGATCCCT CTACAACTTC TCAGGGGAAC AGATGGCGTC CTACTTCGGC 
TATGCAGTGG CTGCCACAGA CGTCAACGGG GACGGGCTGG ACGACTTGCT GGTGGGGGCA 
CCTCTGCTCA TGGAACGAAC AGCTGATGGG CGGCCTCAGG AAGTGGGCAG GGTCTACATC 
TACCTGCAGA ACCCGGCTGG CATGGAGCCC ACCCCCACCC TCACCCTCAC TGGCCATGAT 
GAGTTTGGCC GATTTGGCAG CTCCTTGACG CCCCTGGGGG ACCTGGACCA GGATGGCTAC 
AATGATGTAG CGATTGGAGC TCCCTTTGGT GGAGACACCC AGCAGGGAGT GGTGTTTATA 
TTCCCTGGGG GCCCAGCGGG GCTAGGCTCT AAGCCTTCCC AGGTTCTGCT GCCCCTGTGG 
GCAGCCGGCC ACACGCCAAA CTTCTTTGGC TCTGCCCTTC GAGGAGGCCG AGACCTGGAT 
GGCAACGGAT ACCCTGATCT GATCGTGGGG TCCTTTGGTG TGGACAAGGC TGTGGTGTAC 
AGGGGTCGCC CCATCGTCTC TACCAGTGCC TCCCTCACCA TCTTCCCTGC CATGTTCAAC 
CCAGAGGAGC GTAGCTGCAG CTTGGAGGGG AACCCTGTGG CTTGCATCAA CCTTAGCTTC 
TGCCTCAATG CTTCTGGAAA ACATGTTCCT GACTCCATTG GCTTCACGGT AGAACTCCAG 
TTGGACTGGC AGAAGCAGAA GGGGGGAGTA CGGCGGGCAC TGTTCCTGGC CTCCAGACAG 
GCGACCCTGA CCCAGACCCT ACTCATCCAG AACGGGGCAC GGGAGGACTG CAGAGAGATG 
AAGATCTACC TCAGGAACGA GTCAGAATTC CGAGACAAGC TCTCCCCGAT TCATATCGCC 
CTTAACTTCT CCCTGGACCC CCAAGCCCCT GTGGACAGCC ATGGCCTCCG GCCAGTCTTA 
CATTACCAGA GCAAGAGCCG CATCGAGGAC AAGGCTCAGA TCTTGCTGGA CTGTGGAGAA 
GACAATATCT GTGTGCCAGA CCTACAGCTG GAAGTGTTTG GGGAGCAGAA CCACGTGTAC 
CTGGGTGACA AGAACTCCCT GAACCTAACT TTCCACGCCC AGAACGTGGG TGAGGGCGGC 
GCCTATGAGG CCGAGCTTCG GGTCACAGCC CCTCCAGAGG CTGAGTACTC AGGACTCGTC 
AGAAACCCAG GGAACTTCTC CAGCCTGAGC TGTGACTACT TTGCCGTGAA CCAGAGCCGC 
CTGCTGGTGT GTGACCTGGG AAACCCCATG AAGGCAGGAG CCAGTGTAAG TCTGGCACAG 
GAATCTGGGG GGGGGCTGCG GTTCACAGTC CCTCATCTCC GGGACACAAA GAAAACCATC 
CAGTTTGACT TCCAGATTCT CAGCAAGAAT CTCAACAACT CCCAAAGCGA AGTGGTTTCC 
TTCAGGCTCT CAGTGGAGGC TCAGGCCCAG GTCTCCCTTA ACGGTGTCTC CAAACCCGAG 
GCAGTGCTAT TCCCAGTGAG CGACTGGCAT CCCCGAGACC AGCCTCAGGA GGAGGGAGAT 
GTGGGTCCTG CTGTCCACCA CGTCTATGAG CTCATCAACT TGGGCCCCAG CTCCATTAGC 
CGGGGTGTGC TGGAACTCAG CTGTCCCCAG GCTCTGGAAG GTCAGCAGCT TCTCTACATG 
ACCAGGGTTA CAGGACTTGG TAACTGCACC ACCAGTCACC CCCCCAACTC ACAGGGCCTG 
GAGTTGGATC CTGAGGGTTC CCAGCACCAC CTGCTACAGA GACGGGAAGC TCCAGGCCGG 
AGCCCTGCCT CCTCAGGACC TCAGATCTTG AAATGCCCCG AGGCAGAGTG TTTTAGGCTG 
CGTTGTGAGC TCGGGCCACT GCACCGGCAA GAGAGCCGAA GTCTGCAACT GCATTTTCGA 
GTGTGGGCCA AGACCTTCCT GCAACGGGAG CACCAGCCAT TCAGCCTGCA GTGTGAAGCC 
GTGTATGAAG CCCTGAAGAT GCCTTACCGA ATCCTGCCTC AGCAGCTTCC CCGAAAGGAA 
CTTCAGGTGG CCACGGCTGT GCAGTGGACC AAGGCAGAAG GCAGCCACGG TGTCCCACTG 
TGGATCATTA TCCTAGCCAT CCTCATTGGC CTCCTGCTCC TAGGTCTGCT CATCTACATC 
CTCTACAAGC TCGGATTCTT CAAACGCTCC CTCCCATACG GCACCGCCAT GGAAAAAGCT 
CAACTCAAGC CACCAGCCAC CTCTGATGCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_026892158.1
CDS	77..3409
Translation	MPPLSPPRPAGGGLSREFGKLLPALSHSPLGGLGSGSGSVAPGQGRAGAMGRRAPGFPLHAVQLRWGPRRRPPLLLLLPPLLLLLLPPPPRVGGFNLDAEAPAVLAGPPGSFFGFSVEFYRPGTDGVSVLVGAPKANTSQPGVLQGGAVYLCPWGTSPVHCTPIEFDSKGSRLLESLLPSPEGEEPVEYKSLQWFGATVRAHGSSILACAPLYSWRTEKEPLNDPVGTCYLSTDNFTRILEYAPCRSDFSWAAGQGYCQGGFSAEFTKTGRVVLGGPGSYFWQGQILSATQEQIAESYYPEYLINLVQGQLQTRQASSIYDDSYLGYSVAVGEFSGDDTEDFVAGVPKGNLTYGYVTILNGSDIRSLYNFSGEQMASYFGYAVAATDVNGDGLDDLLVGAPLLMERTADGRPQEVGRVYIYLQNPAGMEPTPTLTLTGHDEFGRFGSSLTPLGDLDQDGYNDVAIGAPFGGDTQQGVVFIFPGGPAGLGSKPSQVLLPLWAAGHTPNFFGSALRGGRDLDGNGYPDLIVGSFGVDKAVVYRGRPIVSTSASLTIFPAMFNPEERSCSLEGNPVACINLSFCLNASGKHVPDSIGFTVELQLDWQKQKGGVRRALFLASRQATLTQTLLIQNGAREDCREMKIYLRNESEFRDKLSPIHIALNFSLDPQAPVDSHGLRPVLHYQSKSRIEDKAQILLDCGEDNICVPDLQLEVFGEQNHVYLGDKNSLNLTFHAQNVGEGGAYEAELRVTAPPEAEYSGLVRNPGNFSSLSCDYFAVNQSRLLVCDLGNPMKAGASVSLAQESGGGLRFTVPHLRDTKKTIQFDFQILSKNLNNSQSEVVSFRLSVEAQAQVSLNGVSKPEAVLFPVSDWHPRDQPQEEGDVGPAVHHVYELINLGPSSISRGVLELSCPQALEGQQLLYMTRVTGLGNCTTSHPPNSQGLELDPEGSQHHLLQRREAPGRSPASSGPQILKCPEAECFRLRCELGPLHRQESRSLQLHFRVWAKTFLQREHQPFSLQCEAVYEALKMPYRILPQQLPRKELQVATAVQWTKAEGSHGVPLWIIILAILIGLLLLGLLIYILYKLGFFKRSLPYGTAMEKAQLKPPATSDA

Target ORF information:

RefSeq Version	XM_027036357.1
Organism	Acinonyx jubatus(cheetah)
Definition	Acinonyx jubatus integrin subunit alpha 5 (ITGA5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_027036357.1

ORF Insert Sequence:

ATGCCCCCCC TCTCGCCCCC CCGGCCGGCG GGGGGAGGGC TCAGCCGGGA GTTTGGCAAA 
CTCCTCCCCG CGTTGAGTCA TTCGCCTCTG GGAGGTTTAG GAAGCGGCTC CGGGTCGGTG 
GCCCCAGGAC AGGGAAGAGC GGGCGCTATG GGGAGACGGG CACCAGGGTT CCCTCTCCAC 
GCCGTGCAAC TGCGCTGGGG CCCCCGGCGC CGACCCCCGC TGCTGCTGCT GCTGCCGCCG 
CTGCTGCTGC TGCTGCTGCC GCCGCCACCC AGGGTCGGGG GCTTCAATTT AGACGCGGAG 
GCCCCAGCTG TGCTCGCTGG GCCCCCGGGC TCCTTCTTCG GCTTCTCGGT GGAGTTTTAC 
CGGCCGGGGA CGGACGGGGT CAGCGTGCTG GTGGGAGCTC CCAAGGCCAA CACCAGCCAG 
CCGGGAGTGC TGCAGGGTGG TGCCGTCTAC CTCTGTCCCT GGGGCACCAG CCCGGTACAC 
TGCACCCCCA TTGAATTTGA CAGTAAAGGC TCTCGACTCC TGGAGTCATT ACTGCCCAGC 
CCAGAGGGAG AGGAGCCTGT GGAGTACAAG TCCTTGCAGT GGTTTGGAGC GACGGTTCGA 
GCCCACGGCT CCTCCATTTT GGCCTGTGCT CCTCTATATA GCTGGCGCAC AGAGAAGGAG 
CCGCTGAATG ATCCCGTGGG CACCTGCTAC CTCTCCACGG ACAATTTCAC CCGAATTCTG 
GAGTATGCAC CTTGCCGCTC AGATTTCAGC TGGGCCGCAG GACAGGGTTA CTGCCAAGGG 
GGCTTCAGTG CTGAGTTCAC CAAGACTGGG CGTGTGGTCC TGGGTGGACC AGGGAGCTAC 
TTCTGGCAAG GCCAGATCCT GTCGGCCACC CAAGAACAGA TTGCCGAATC CTATTACCCC 
GAGTACCTGA TCAACCTGGT TCAGGGGCAG CTGCAGACCC GCCAGGCCAG TTCCATCTAC 
GATGACAGCT ACCTGGGATA CTCTGTGGCT GTGGGTGAAT TCAGTGGTGA TGACACAGAA 
GACTTTGTCG CTGGCGTGCC CAAAGGGAAC CTCACTTATG GTTATGTCAC CATCCTCAAT 
GGCTCAGACA TCCGATCCCT CTACAACTTC TCAGGGGAAC AGATGGCGTC CTACTTCGGC 
TATGCAGTGG CTGCCACAGA CGTCAACGGG GACGGGCTGG ACGACTTGCT GGTGGGGGCA 
CCTCTGCTCA TGGAACGAAC AGCTGATGGG CGGCCTCAGG AAGTGGGCAG GGTCTACATC 
TACCTGCAGA ACCCGGCTGG CATGGAGCCC ACCCCCACCC TCACCCTCAC TGGCCATGAT 
GAGTTTGGCC GATTTGGCAG CTCCTTGACG CCCCTGGGGG ACCTGGACCA GGATGGCTAC 
AATGATGTAG CGATTGGAGC TCCCTTTGGT GGAGACACCC AGCAGGGAGT GGTGTTTATA 
TTCCCTGGGG GCCCAGCGGG GCTAGGCTCT AAGCCTTCCC AGGTTCTGCT GCCCCTGTGG 
GCAGCCGGCC ACACGCCAAA CTTCTTTGGC TCTGCCCTTC GAGGAGGCCG AGACCTGGAT 
GGCAACGGAT ACCCTGATCT GATCGTGGGG TCCTTTGGTG TGGACAAGGC TGTGGTGTAC 
AGGGGTCGCC CCATCGTCTC TACCAGTGCC TCCCTCACCA TCTTCCCTGC CATGTTCAAC 
CCAGAGGAGC GTAGCTGCAG CTTGGAGGGG AACCCTGTGG CTTGCATCAA CCTTAGCTTC 
TGCCTCAATG CTTCTGGAAA ACATGTTCCT GACTCCATTG GCTTCACGGT AGAACTCCAG 
TTGGACTGGC AGAAGCAGAA GGGGGGAGTA CGGCGGGCAC TGTTCCTGGC CTCCAGACAG 
GCGACCCTGA CCCAGACCCT ACTCATCCAG AACGGGGCAC GGGAGGACTG CAGAGAGATG 
AAGATCTACC TCAGGAACGA GTCAGAATTC CGAGACAAGC TCTCCCCGAT TCATATCGCC 
CTTAACTTCT CCCTGGACCC CCAAGCCCCT GTGGACAGCC ATGGCCTCCG GCCAGTCTTA 
CATTACCAGA GCAAGAGCCG CATCGAGGAC AAGGCTCAGA TCTTGCTGGA CTGTGGAGAA 
GACAATATCT GTGTGCCAGA CCTACAGCTG GAAGTGTTTG GGGAGCAGAA CCACGTGTAC 
CTGGGTGACA AGAACTCCCT GAACCTAACT TTCCACGCCC AGAACGTGGG TGAGGGCGGC 
GCCTATGAGG CCGAGCTTCG GGTCACAGCC CCTCCAGAGG CTGAGTACTC AGGACTCGTC 
AGAAACCCAG GGAACTTCTC CAGCCTGAGC TGTGACTACT TTGCCGTGAA CCAGAGCCGC 
CTGCTGGTGT GTGACCTGGG AAACCCCATG AAGGCAGGAG CCAGTGTAAG TCTGGCACAG 
GAATCTGGGG GGGGGCTGCG GTTCACAGTC CCTCATCTCC GGGACACAAA GAAAACCATC 
CAGTTTGACT TCCAGATTCT CAGCAAGAAT CTCAACAACT CCCAAAGCGA AGTGGTTTCC 
TTCAGGCTCT CAGTGGAGGC TCAGGCCCAG GTCTCCCTTA ACGGTGTCTC CAAACCCGAG 
GCAGTGCTAT TCCCAGTGAG CGACTGGCAT CCCCGAGACC AGCCTCAGGA GGAGGGAGAT 
GTGGGTCCTG CTGTCCACCA CGTCTATGAG CTCATCAACT TGGGCCCCAG CTCCATTAGC 
CGGGGTGTGC TGGAACTCAG CTGTCCCCAG GCTCTGGAAG GTCAGCAGCT TCTCTACATG 
ACCAGGGTTA CAGGACTTGG TAACTGCACC ACCAGTCACC CCCCCAACTC ACAGGGCCTG 
GAGTTGGATC CTGAGGGTTC CCAGCACCAC CTGCTACAGA GACGGGAAGC TCCAGGCCGG 
AGCCCTGCCT CCTCAGGACC TCAGATCTTG AAATGCCCCG AGGCAGAGTG TTTTAGGCTG 
CGTTGTGAGC TCGGGCCACT GCACCGGCAA GAGAGCCGAA GTCTGCAACT GCATTTTCGA 
GTGTGGGCCA AGACCTTCCT GCAACGGGAG CACCAGCCAT TCAGCCTGCA GTGTGAAGCC 
GTGTATGAAG CCCTGAAGAT GCCTTACCGA ATCCTGCCTC AGCAGCTTCC CCGAAAGGAA 
CTTCAGGTGG CCACGGCTGT GCAGTGGACC AAGGCAGAAG GCAGCCACGG TGTCCCACTG 
TGGATCATTA TCCTAGCCAT CCTCATTGGC CTCCTGCTCC TAGGTCTGCT CATCTACATC 
CTCTACAAGC TCGGATTCTT CAAACGCTCC CTCCCATACG GCACCGCCAT GGAAAAAGCT 
CAACTCAAGC CACCAGCCAC CTCTGATGCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITGA5 ( XM_015087803.1 ) cDNA ORF clone, Acinonyx jubatus(cheetah) -> XP_014943289.1 Acinonyx jubatus integrin alpha 5 (ITGA5), mRNA.

Price & Availability↑

CloneID	OAc57592
Clone ID Related Accession (Same CDS sequence)	XM_015087803.1
Accession Version	XM_015087803.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3288bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-14
Organism	Acinonyx jubatus(cheetah)
Product	integrin alpha-5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015132219.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Acinonyx jubatus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 4% of CDS bases ##RefSeq-Attributes-END##

ATGATATTTG CACAGTCCAC CAGGGCCAGC TTCCAGACCT GGAGGGCTGT GCTGAAGCCG 
TTCCAACTAG GAGGGAACCC TAAGCCAGGC CTGACCCGGA AGCAGAGGAA AGCAGAGCCA 
GGCAAAGGCA GGCATGGGAA GGTGGTGGAG GGGGGTGGTT GTTGTGTTAG TGACAGCGCC 
ACATGGGGAC CGCCTGACTC CAGCCTGTGC CTCTGGCATC TGTGGCATCT TCTGAGATAT 
AAATACAACC CAGGGGGCTT CTCCCCCACC CCCGGCCAGC CCCTGGCCCA GAGGCCGGGG 
GGGACTAGAG AAGGAGTCAC TCTGAACCGC CTCTCCTTTC GCCTCCACAG GGTCAGCGTG 
CTGGTGGGAG CTCCCAAGGC CAACACCAGC CAGCCGGGAG TGCTGCAGGG TGGTGCCGTC 
TACCTCTGTC CCTGGGGCAC CAGCCCGGTA CACTGCACCC CCATTGAATT TGACAGTAAA 
GGCTCTCGAC TCCTGGAGTC ATTACTGCCC AGCCCAGAGG GAGAGGAGCC TGTGGAGTAC 
AAGTCCTTGC AGTGGTTTGG AGCGACGGTT CGAGCCCACG GCTCCTCCAT TTTGGCCTGT 
GCTCCTCTAT ATAGCTGGCG CACAGAGAAG GAGCCGCTGA ATGATCCCGT GGGCACCTGC 
TACCTCTCCA CGGACAATTT CACCCGAATT CTGGAGTATG CACCTTGCCG CTCAGATTTC 
AGCTGGGCCG CAGGACAGGG TTACTGCCAA GGGGGCTTCA GTGCTGAGTT CACCAAGACT 
GGGCGTGTGG TCCTGGGTGG ACCAGGGAGC TACTTCTGGC AAGGCCAGAT CCTGTCGGCC 
ACCCAAGAAC AGATTGCCGA ATCCTATTAC CCCGAGTACC TGATCAACCT GGTTCAGGGG 
CAGCTGCAGA CCCGCCAGGC CAGTTCCATC TACGATGACA GCTACCTGGG ATACTCTGTG 
GCTGTGGGTG AATTCAGTGG TGATGACACA GAAGACTTTG TCGCTGGCGT GCCCAAAGGG 
AACCTCACTT ATGGTTATGT CACCATCCTC AATGGCTCAG ACATCCGATC CCTCTACAAC 
TTCTCAGGGG AACAGATGGC GTCCTACTTC GGTAATGCAG TGGCTGCCAC AGACGTCAAC 
GGGGACGGGC TGGACGACTT GCTGGTGGGG GCACCTCTGC TCATGGAACG AACAGCTGAT 
GGGCGGCCTC AGGAAGTGGG CAGGGTCTAC ATCTACCTGC AGAACCCGGC TGGCATGGAG 
CCCACCCCCA CCCTCACCCT CACTGGCCAT GATGAGTTTG GCCGATTTGG CAGCTCCTTG 
ACGCCCCTGG GGGACCTGGA CCAGGATGGC TACAATGATG TAGCGATTGG AGCTCCCTTT 
GGTGGAGACA CCCAGCAGGG AGTGGTGTTT ATATTCCCTG GGGGCCCAGC GGGGCTAGGC 
TCTAAGCCTT CCCAGGTTCT GCTGCCCCTG TGGGCAGCCG GCCACACGCC AAACTTCTTT 
GGCTCTGCCC TTCGAGGAGG CCGAGACCTG GATGGCAACG GATACCCTGA TCTGATCGTG 
GGGTCCTTTG GTGTGGACAA GGCTGTGGTG TACAGGGGTC GCCCCATCGT CTCTACCAGT 
GCCTCCCTCA CCATCTTCCC TGCCATGTTC AACCCAGAGG AGCGTAGCTG CAGCTTGGAG 
GGGAACCCTG TGGCTTGCAT CAACCTTAGC TTCTGCCTCA ATGCTTCTGG AAAACATGTT 
CCTGACTCCA TTGGCTTCAC GGTAGAACTC CAGTTGGACT GGCAGAAGCA GAAGGGGGGA 
GTACGGCGGG CACTGTTCCT GGCCTCCAGA CAGGCGACCC TGACCCAGAC CCTACTCATC 
CAGAACGGGG CACGGGAGGA CTGCAGAGAG ATGAAGATCT ACCTCAGGAA CGAGTCAGAA 
TTCCGAGACA AGCTCTCCCC GATTCATATC GCCCTTAACT TCTCCCTGGA CCCCCAAGCC 
CCTGTGGACA GCCATGGCCT CCGGCCAGTC TTACATTACC AGAGCAAGAG CCGCATCGAG 
GACAAGGCTC AGATCTTGCT GGACTGTGGA GAAGACAATA TCTGTGTGCC AGACCTACAG 
CTGGAAGTGT TTGGGGAGCA GAACCACGTG TACCTGGGTG ACAAGAACTC CCTGAACCTA 
ACTTTCCACG CCCAGAACGT GGGTGAGGGC GGCGCCTATG AGGCCGAGCT TCGGGTCACA 
GCCCCTCCAG AGGCTGAGTA CTCAGGACTC GTCAGAAACC CAGGGAACTT CTCCAGCCTG 
AGCTGTGACT ACTTTGCCGT GAACCAGAGC CGCCTGCTGG TGTGTGACCT GGGAAACCCC 
ATGAAGGCAG GAGCCAGTAT CTGGGGGGGG CTGCGGTTCA CAGTCCCTCA TCTCCGGGAC 
ACAAAGAAAA CCATCCAGTT TGACTTCCAG ATTCTCAGCA AGAATCTCAA CAACTCCCAA 
AGCGAAGTGG TTTCCTTCAG GCTCTCAGTG GAGGCTCAGG CCCAGGTCTC CCTTAACGGT 
GTCTCCAAAC CCGAGGCAGT GCTATTCCCA GTGAGCGACT GGCATCCCCG AGACCAGCCT 
CAGGAGGAGG GAGATGTGGG TCCTGCTGTC CACCACGTCT ATGAGCTCAT CAACTTGGGC 
CCCAGCTCCA TTAGCCGGGG TGTGCTGGAA CTCAGCTGTC CCCAGGCTCT GGAAGGTCAG 
CAGCTTCTCT ACATGACCAG GGTTACAGGA CTTGGTAACT GCACCACCAG TCACCCCCCC 
AACTCACAGG GCCTGGAGTT GGATCCTGAG GGTTCCCAGC ACCACCTGCT ACAGAGACGG 
GAAGCTCCAG GCCGGAGCCC TGCCTCCTCA GGACCTCAGA TCTTGAAATG CCCCGAGGCA 
GAGTGTTTTA GGCTGCGTTG TGAGCTCGGG CCACTGCACC GGCAAGAGAG CCGAAGTCTG 
CAACTGCATT TTCGAGTGTG GGCCAAGACC TTCCTGCAAC GGGAGCACCA GCCATTCAGC 
CTGCAGTGTG AAGCCGTGTA TGAAGCCCTG AAGATGCCTT ACCGAATCCT GCCTCAGCAG 
CTTCCCCGAA AGGAACTTCA GGTGGCCACG GCTGTGCAGT GGACCAAGGC AGAAGGCAGC 
CACGGTGTCC CACTGTGGAT CATTATCCTA GCCATCCTCA TTGGCCTCCT GCTCCTAGGT 
CTGCTCATCT ACATCCTCTA CAAGCTCGGA TTCTTCAAAC GCTCCCTCCC ATACGGCACC 
GCCATGGAAA AAGCTCAACT CAAGCCACCA GCCACCTCTG ATGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014943289.1
CDS	1..3288
Translation	MIFAQSTRASFQTWRAVLKPFQLGGNPKPGLTRKQRKAEPGKGRHGKVVEGGGCCVSDSATWGPPDSSLCLWHLWHLLRYKYNPGGFSPTPGQPLAQRPGGTREGVTLNRLSFRLHRVSVLVGAPKANTSQPGVLQGGAVYLCPWGTSPVHCTPIEFDSKGSRLLESLLPSPEGEEPVEYKSLQWFGATVRAHGSSILACAPLYSWRTEKEPLNDPVGTCYLSTDNFTRILEYAPCRSDFSWAAGQGYCQGGFSAEFTKTGRVVLGGPGSYFWQGQILSATQEQIAESYYPEYLINLVQGQLQTRQASSIYDDSYLGYSVAVGEFSGDDTEDFVAGVPKGNLTYGYVTILNGSDIRSLYNFSGEQMASYFGNAVAATDVNGDGLDDLLVGAPLLMERTADGRPQEVGRVYIYLQNPAGMEPTPTLTLTGHDEFGRFGSSLTPLGDLDQDGYNDVAIGAPFGGDTQQGVVFIFPGGPAGLGSKPSQVLLPLWAAGHTPNFFGSALRGGRDLDGNGYPDLIVGSFGVDKAVVYRGRPIVSTSASLTIFPAMFNPEERSCSLEGNPVACINLSFCLNASGKHVPDSIGFTVELQLDWQKQKGGVRRALFLASRQATLTQTLLIQNGAREDCREMKIYLRNESEFRDKLSPIHIALNFSLDPQAPVDSHGLRPVLHYQSKSRIEDKAQILLDCGEDNICVPDLQLEVFGEQNHVYLGDKNSLNLTFHAQNVGEGGAYEAELRVTAPPEAEYSGLVRNPGNFSSLSCDYFAVNQSRLLVCDLGNPMKAGASIWGGLRFTVPHLRDTKKTIQFDFQILSKNLNNSQSEVVSFRLSVEAQAQVSLNGVSKPEAVLFPVSDWHPRDQPQEEGDVGPAVHHVYELINLGPSSISRGVLELSCPQALEGQQLLYMTRVTGLGNCTTSHPPNSQGLELDPEGSQHHLLQRREAPGRSPASSGPQILKCPEAECFRLRCELGPLHRQESRSLQLHFRVWAKTFLQREHQPFSLQCEAVYEALKMPYRILPQQLPRKELQVATAVQWTKAEGSHGVPLWIIILAILIGLLLLGLLIYILYKLGFFKRSLPYGTAMEKAQLKPPATSDA

Target ORF information:

RefSeq Version	XM_015087803.1
Organism	Acinonyx jubatus(cheetah)
Definition	Acinonyx jubatus integrin alpha 5 (ITGA5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015087803.1

ORF Insert Sequence:

ATGATATTTG CACAGTCCAC CAGGGCCAGC TTCCAGACCT GGAGGGCTGT GCTGAAGCCG 
TTCCAACTAG GAGGGAACCC TAAGCCAGGC CTGACCCGGA AGCAGAGGAA AGCAGAGCCA 
GGCAAAGGCA GGCATGGGAA GGTGGTGGAG GGGGGTGGTT GTTGTGTTAG TGACAGCGCC 
ACATGGGGAC CGCCTGACTC CAGCCTGTGC CTCTGGCATC TGTGGCATCT TCTGAGATAT 
AAATACAACC CAGGGGGCTT CTCCCCCACC CCCGGCCAGC CCCTGGCCCA GAGGCCGGGG 
GGGACTAGAG AAGGAGTCAC TCTGAACCGC CTCTCCTTTC GCCTCCACAG GGTCAGCGTG 
CTGGTGGGAG CTCCCAAGGC CAACACCAGC CAGCCGGGAG TGCTGCAGGG TGGTGCCGTC 
TACCTCTGTC CCTGGGGCAC CAGCCCGGTA CACTGCACCC CCATTGAATT TGACAGTAAA 
GGCTCTCGAC TCCTGGAGTC ATTACTGCCC AGCCCAGAGG GAGAGGAGCC TGTGGAGTAC 
AAGTCCTTGC AGTGGTTTGG AGCGACGGTT CGAGCCCACG GCTCCTCCAT TTTGGCCTGT 
GCTCCTCTAT ATAGCTGGCG CACAGAGAAG GAGCCGCTGA ATGATCCCGT GGGCACCTGC 
TACCTCTCCA CGGACAATTT CACCCGAATT CTGGAGTATG CACCTTGCCG CTCAGATTTC 
AGCTGGGCCG CAGGACAGGG TTACTGCCAA GGGGGCTTCA GTGCTGAGTT CACCAAGACT 
GGGCGTGTGG TCCTGGGTGG ACCAGGGAGC TACTTCTGGC AAGGCCAGAT CCTGTCGGCC 
ACCCAAGAAC AGATTGCCGA ATCCTATTAC CCCGAGTACC TGATCAACCT GGTTCAGGGG 
CAGCTGCAGA CCCGCCAGGC CAGTTCCATC TACGATGACA GCTACCTGGG ATACTCTGTG 
GCTGTGGGTG AATTCAGTGG TGATGACACA GAAGACTTTG TCGCTGGCGT GCCCAAAGGG 
AACCTCACTT ATGGTTATGT CACCATCCTC AATGGCTCAG ACATCCGATC CCTCTACAAC 
TTCTCAGGGG AACAGATGGC GTCCTACTTC GGTAATGCAG TGGCTGCCAC AGACGTCAAC 
GGGGACGGGC TGGACGACTT GCTGGTGGGG GCACCTCTGC TCATGGAACG AACAGCTGAT 
GGGCGGCCTC AGGAAGTGGG CAGGGTCTAC ATCTACCTGC AGAACCCGGC TGGCATGGAG 
CCCACCCCCA CCCTCACCCT CACTGGCCAT GATGAGTTTG GCCGATTTGG CAGCTCCTTG 
ACGCCCCTGG GGGACCTGGA CCAGGATGGC TACAATGATG TAGCGATTGG AGCTCCCTTT 
GGTGGAGACA CCCAGCAGGG AGTGGTGTTT ATATTCCCTG GGGGCCCAGC GGGGCTAGGC 
TCTAAGCCTT CCCAGGTTCT GCTGCCCCTG TGGGCAGCCG GCCACACGCC AAACTTCTTT 
GGCTCTGCCC TTCGAGGAGG CCGAGACCTG GATGGCAACG GATACCCTGA TCTGATCGTG 
GGGTCCTTTG GTGTGGACAA GGCTGTGGTG TACAGGGGTC GCCCCATCGT CTCTACCAGT 
GCCTCCCTCA CCATCTTCCC TGCCATGTTC AACCCAGAGG AGCGTAGCTG CAGCTTGGAG 
GGGAACCCTG TGGCTTGCAT CAACCTTAGC TTCTGCCTCA ATGCTTCTGG AAAACATGTT 
CCTGACTCCA TTGGCTTCAC GGTAGAACTC CAGTTGGACT GGCAGAAGCA GAAGGGGGGA 
GTACGGCGGG CACTGTTCCT GGCCTCCAGA CAGGCGACCC TGACCCAGAC CCTACTCATC 
CAGAACGGGG CACGGGAGGA CTGCAGAGAG ATGAAGATCT ACCTCAGGAA CGAGTCAGAA 
TTCCGAGACA AGCTCTCCCC GATTCATATC GCCCTTAACT TCTCCCTGGA CCCCCAAGCC 
CCTGTGGACA GCCATGGCCT CCGGCCAGTC TTACATTACC AGAGCAAGAG CCGCATCGAG 
GACAAGGCTC AGATCTTGCT GGACTGTGGA GAAGACAATA TCTGTGTGCC AGACCTACAG 
CTGGAAGTGT TTGGGGAGCA GAACCACGTG TACCTGGGTG ACAAGAACTC CCTGAACCTA 
ACTTTCCACG CCCAGAACGT GGGTGAGGGC GGCGCCTATG AGGCCGAGCT TCGGGTCACA 
GCCCCTCCAG AGGCTGAGTA CTCAGGACTC GTCAGAAACC CAGGGAACTT CTCCAGCCTG 
AGCTGTGACT ACTTTGCCGT GAACCAGAGC CGCCTGCTGG TGTGTGACCT GGGAAACCCC 
ATGAAGGCAG GAGCCAGTAT CTGGGGGGGG CTGCGGTTCA CAGTCCCTCA TCTCCGGGAC 
ACAAAGAAAA CCATCCAGTT TGACTTCCAG ATTCTCAGCA AGAATCTCAA CAACTCCCAA 
AGCGAAGTGG TTTCCTTCAG GCTCTCAGTG GAGGCTCAGG CCCAGGTCTC CCTTAACGGT 
GTCTCCAAAC CCGAGGCAGT GCTATTCCCA GTGAGCGACT GGCATCCCCG AGACCAGCCT 
CAGGAGGAGG GAGATGTGGG TCCTGCTGTC CACCACGTCT ATGAGCTCAT CAACTTGGGC 
CCCAGCTCCA TTAGCCGGGG TGTGCTGGAA CTCAGCTGTC CCCAGGCTCT GGAAGGTCAG 
CAGCTTCTCT ACATGACCAG GGTTACAGGA CTTGGTAACT GCACCACCAG TCACCCCCCC 
AACTCACAGG GCCTGGAGTT GGATCCTGAG GGTTCCCAGC ACCACCTGCT ACAGAGACGG 
GAAGCTCCAG GCCGGAGCCC TGCCTCCTCA GGACCTCAGA TCTTGAAATG CCCCGAGGCA 
GAGTGTTTTA GGCTGCGTTG TGAGCTCGGG CCACTGCACC GGCAAGAGAG CCGAAGTCTG 
CAACTGCATT TTCGAGTGTG GGCCAAGACC TTCCTGCAAC GGGAGCACCA GCCATTCAGC 
CTGCAGTGTG AAGCCGTGTA TGAAGCCCTG AAGATGCCTT ACCGAATCCT GCCTCAGCAG 
CTTCCCCGAA AGGAACTTCA GGTGGCCACG GCTGTGCAGT GGACCAAGGC AGAAGGCAGC 
CACGGTGTCC CACTGTGGAT CATTATCCTA GCCATCCTCA TTGGCCTCCT GCTCCTAGGT 
CTGCTCATCT ACATCCTCTA CAAGCTCGGA TTCTTCAAAC GCTCCCTCCC ATACGGCACC 
GCCATGGAAA AAGCTCAACT CAAGCCACCA GCCACCTCTG ATGCCTGA