HECW1 cDNA ORF clone, Acinonyx jubatus(cheetah)

Gene
Clones
gRNAs

Summary

Gene Symbol	HECW1
Entrez Gene ID	106977749
Full Name	HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1
Gene Type	protein-coding
Organism	Acinonyx jubatus(cheetah)

mRNA and Protein(s)

mRNA	Protein	Name
XM_027071594.1	XP_026927395.1	E3 ubiquitin-protein ligase HECW1 isoform X3
XM_027071595.1	XP_026927396.1	E3 ubiquitin-protein ligase HECW1 isoform X4
XM_015075294.1	XP_014930780.1	E3 ubiquitin-protein ligase HECW1
XM_027071591.1	XP_026927392.1	E3 ubiquitin-protein ligase HECW1 isoform X1
XM_027071593.1	XP_026927394.1	E3 ubiquitin-protein ligase HECW1 isoform X2
XM_027071596.1	XP_026927397.1	E3 ubiquitin-protein ligase HECW1 isoform X4

Pathways from BioSystems
Gene Ontology

The following HECW1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HECW1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OAc48186	XM_015075294.1 Latest version!	Acinonyx jubatus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OAc228957	XM_027071591.1 Latest version!	Acinonyx jubatus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OAc228958	XM_027071593.1 Latest version!	Acinonyx jubatus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OAc228959	XM_027071594.1 Latest version!	Acinonyx jubatus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OAc228960	XM_027071596.1 Latest version!	Acinonyx jubatus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OAc228960	XM_027071595.1 Latest version!	Acinonyx jubatus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

HECW1 ( XM_015075294.1 ) cDNA ORF clone, Acinonyx jubatus(cheetah) -> XP_014930780.1 Acinonyx jubatus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), mRNA.

Price & Availability↑

CloneID	OAc48186
Clone ID Related Accession (Same CDS sequence)	XM_015075294.1
Accession Version	XM_015075294.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3861bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-12-14
Organism	Acinonyx jubatus(cheetah)
Product	E3 ubiquitin-protein ligase HECW1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015131101.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Acinonyx jubatus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

ATGCTGCTGC ACCTCTGTAG TGTGAAGAAT CTGTACCAGA ACAGGTTTTT AGGCCTGGCC 
GCCATGGCAT CCCCATCTAG AAACTCCCAG AATCGCCGCC GGTGCAAGGA GCCACTCCGT 
CACAGCTACA ACCCTGGCCA GTTCCACAGC ATGGCCATCA GGACCGGCCC CCACGGCACC 
GTCACCATAC CCCGCTCCAC CAGCGACACC GACCTGGTCA CCTCAGACAG CCGCTCCACA 
CTTATGGTCA GCAGCTCCTA CTACTCCATA GGACACTCTC AGGACCTGGT GATCCACTGG 
GACATCAAGG AGGAAGTGGA TGCTGGAGAC TGGATTGGCA TGTATCTCAT TGATGAGGTC 
TTGTCAGAAA ACTTTTTGGA CTATAAGAAC CGTGGAGTCA ATGGTTCTCA TCGGGGCCAG 
ATCATCTGGA AGATTGATGC CAGCTCTTAC TTTGTGGAAC CCGAAACTAA GATCTGCTTC 
AAATACTACC ATGGCGTGAG TGGAGCCCTG CGAGCCACCA CCCCCAGTGT CACGGTCAAA 
AACTCTGCAG CTCCTATTTT TAAAAGCATT GGCTCTGATG AGACCGTCCA AGGGCAAGGA 
AGTCGGAGGC TGATCAGCTT TTCTCTCTCA GATTTCCAGG CCATGGGGCT GAAGAAAGGG 
ATGTTTTTCA ACCCAGACCC TTACCTGAAG ATTTCCATTC AGCCTGGAAA GCACAGCATC 
TTCCCTGCCC TCCCACACCA TGGACAGGAG AGGAGATCCA AGATCATAGG CAACACTGTG 
AACCCCATCT GGCAGGCCGA GCAATTCAGC TTTGTGTCCT TGCCCACTGA TGTACTGGAA 
ATTGAGGTGA AAGACAAGTT TGCTAAGAGC CGCCCCATCA TCAAGCGCTT CTTGGGAAAG 
CTGTCGATGC CCGTTCAAAG ACTCCTGGAG AGACACGCCA TAGGGGATAG GGTGGTCAGC 
TATACACTTG GCCGCAGGCT TCCAACAGAT CATGTGAGTG GACAGCTGCA ATTCCGATTT 
GAGATCACTT CTTCCATCCA CCCAGATGAT GAGGAGATGT CCCTCAGCGC CGAGCCTGAG 
TCCGCGGACA TTCAGGACAG CCCCATGAAC AGCCTGTTGG CCAGCAGCCG TGGGGAGCCT 
CCCGGCGAGG CACCGGAGCT CCCCAGGGGC TCCACGCCCG ACAGCCACAC CCGCTTCTCC 
TCCGTGGACA GCGCCAAGAT CTCTGAGAGC ACCGTCTTCT CGTCGCAAGA CGATGAGGAG 
GAGGAGAACA GTGCGTTCGA ATCTGTGCCC GACTCGGTAC AGAGCCCGGA GCTGGACCCG 
GAGTCGGCAA ATGGCGCCGG GCCGTGGCAC GACGAGCTGG CCGCCCCCGC CGCCAGCGTG 
GCAAGAACCG TGGAAGGTCT GGAATCCCCC GTGGCAGGGC CAAGCAATCG GAGAGAAGGT 
GAATGTCCTA TACTCCATAA TTCCCAGCCA GTAAGCCAGC TTCCTTCCTT GAGGCCTGAA 
CATCATCACT ACCCAACAAT CGATGAGCCT CTTCCACCAA ACTGGGAAGC TCGCATTGAC 
AGCCACGGGC GGGTCTTTTA TGTGGACCAC GTGAACCGCA CGACCACCTG GCAGCGGCCG 
ACAGCAGCAG CCACCCCCGA CGGGATGCGG AGATCGGGGT CCATCCAGCA GATGGAGCAA 
CTCAACAGGC GGTATCAAAA TATTCAGCGA ACCATTGCAA CAGAGAGACC AGAAGAAGAT 
TCTGGAGGCC AAAGTTGTGA GCAGGTCCCA ACAGGAGGCA GTGGAGGCGG TGGGAGCGAC 
TCAGAGGCTG ACTCTTCTCA ATCAAGCTTA GATCTGAGGA GAGAAGGGTC ACTTTCTCCA 
GTGAACTCAC AAAAAATCAC CTTGTTGCTG CAGTCCCCAG CAGTGAAGTT CATTACCAAT 
CCCGAGTTTT TCACTGTACT ACACGCCAAT TATGGTGCCT ACCGAGTCTT CACCAGTAGC 
ACCTGCTTAA AGCACATGAT TCTGAAAGTC CGGCGGGACG CTCGCAATTT CGAACGCTAC 
CAGCACAACC GTGACTTGGT GAATTTCATC AACATGTTTG CAGACACACG GCTGGAATTG 
CCCCGGGGCT GGGAGATCAA AACGGACCAG CAGGGAAAGT CTTTTTTCGT GGACCACAAT 
AGTCGAGCCA CCACTTTCAT TGACCCCCGA ATCCCTCTTC AGAACGGTCG TCTTCCCAAT 
CACCTGACAC ATCGACAGCA CCTCCAGAGG CTCCGGAGTT ACAGCGCTGG AGAGGCTTCA 
GAAGTCTCTC GAAACAGAGG AGCCTCTTTA CTGGCCAGGC CAGGACACAG CCTGGTAGCT 
GCTATTCGAA GCCAACATCA ACATGAGTCA TTGCCACTAG CATATAATGA CAAGATTGTG 
GCATTTCTCC GCCAGCCAAA CATTTTTGAA ATGCTGCAAG AGCGTCAGCC AAGCCTGGCG 
AGAAACCACA CACTCAGGGA GAAAATCCAT TACATTCGGA CTGAGGGTAA TCATGGGCTT 
GAAAAGTTGT CCTGTGATGC AGATCTAGTC ATTTTGCTGA GTCTCTTTGA AGAAGAGATT 
ATGTCCTACG TCCCACTGCA GACTGCCTTT CATCCCGGGT ACAGCTTCTC TCCCCGCTGT 
TCACCCTGTT CCTCACCTCA GAACTCCCCA GGTTTACAGA GAGCCAGTGC AAGAGCCCCT 
TCACCCTACC GGAGAGACTT CGAGGCCAAG CTCCGCAATT TCTACCGAAA ACTGGAAGCC 
AAAGGATTTG GTCAGGGTCC AGGGAAAATT AAGCTCATCA TTCGTCGGGA CCACTTGTTA 
GAAGGAACCT TCAATCAGGT GATGGCCTAT TCCCGGAAGG AGCTTCAGCG AAACAAACTC 
TACGTTACCT TTGTTGGAGA GGAGGGTCTG GACTACAGTG GTCCTTCGCG AGAGTTCTTC 
TTCCTTTTGT CTCAGGAGCT CTTCAACCCT TACTACGGAC TCTTTGAGTA CTCTGCAAAT 
GATACGTACA CGGTGCAGAT CAGCCCCATG TCGGCATTTG TGGAAAACCA TCTTGAATGG 
TTCAGGTTTA GTGGTCGGAT CCTAGGCCTG GCTTTGATCC ATCAGTACCT TCTTGATGCC 
TTCTTCACAA GGCCCTTCTA TAAGGCACTC CTGAGACTGC CCTGTGATTT AAGTGATCTG 
GAATATCTGG ATGAGGAATT TCACCAGAGT TTGCAATGGA TGAAGGACAA CAACATCACA 
GATATCCTAG ACCTCACTTT CACTGTTAAC GAAGAAGTTT TTGGACAGGT AACGGAAAGG 
GAGTTGAAAT CTGGAGGAGC CAACACGCAG GTGACGGAGA AGAACAAGAA GGAGTACATT 
GAGAGGATGG TGAAGTGGCG GGTGGAGCGC GGTGTGGTGC AGCAGACCGA GGCCCTGGTG 
CGGGGCTTCT ATGAGGTGGT AGACTCGAGG CTTGTCTCTG TGTTCGACGC CAGGGAGCTG 
GAGCTGGTGA TAGCAGGCAC GGCGGAAATC GACCTGAATG ACTGGCGGAA TAACACAGAG 
TACCGGGGAG GTTATCATGA CGGGCATCTT GTGATCCGCT GGTTCTGGGC CGCCGTGGAG 
CGCTTCAATA ATGAGCAGAG ACTGAGATTA CTTCAGTTCG TCACGGGAAC GTCAAGCGTG 
CCCTACGAAG GCTTCGCAGC CCTTCGAGGA AGCAATGGGC TTCGGCGTTT CTGCATAGAG 
AAGTGGGGGA AGATTACGTC TCTCCCCAGG GCACACACAT GCTTCAACCG ACTGGATCTT 
CCACCTTATC CCTCATACTC CATGTTATAT GAAAAGCTGC TAACAGCAGT AGAAGAAACC 
AGCACCTTTG GACTTGAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014930780.1
CDS	27..3887
Translation	MLLHLCSVKNLYQNRFLGLAAMASPSRNSQNRRRCKEPLRHSYNPGQFHSMAIRTGPHGTVTIPRSTSDTDLVTSDSRSTLMVSSSYYSIGHSQDLVIHWDIKEEVDAGDWIGMYLIDEVLSENFLDYKNRGVNGSHRGQIIWKIDASSYFVEPETKICFKYYHGVSGALRATTPSVTVKNSAAPIFKSIGSDETVQGQGSRRLISFSLSDFQAMGLKKGMFFNPDPYLKISIQPGKHSIFPALPHHGQERRSKIIGNTVNPIWQAEQFSFVSLPTDVLEIEVKDKFAKSRPIIKRFLGKLSMPVQRLLERHAIGDRVVSYTLGRRLPTDHVSGQLQFRFEITSSIHPDDEEMSLSAEPESADIQDSPMNSLLASSRGEPPGEAPELPRGSTPDSHTRFSSVDSAKISESTVFSSQDDEEEENSAFESVPDSVQSPELDPESANGAGPWHDELAAPAASVARTVEGLESPVAGPSNRREGECPILHNSQPVSQLPSLRPEHHHYPTIDEPLPPNWEARIDSHGRVFYVDHVNRTTTWQRPTAAATPDGMRRSGSIQQMEQLNRRYQNIQRTIATERPEEDSGGQSCEQVPTGGSGGGGSDSEADSSQSSLDLRREGSLSPVNSQKITLLLQSPAVKFITNPEFFTVLHANYGAYRVFTSSTCLKHMILKVRRDARNFERYQHNRDLVNFINMFADTRLELPRGWEIKTDQQGKSFFVDHNSRATTFIDPRIPLQNGRLPNHLTHRQHLQRLRSYSAGEASEVSRNRGASLLARPGHSLVAAIRSQHQHESLPLAYNDKIVAFLRQPNIFEMLQERQPSLARNHTLREKIHYIRTEGNHGLEKLSCDADLVILLSLFEEEIMSYVPLQTAFHPGYSFSPRCSPCSSPQNSPGLQRASARAPSPYRRDFEAKLRNFYRKLEAKGFGQGPGKIKLIIRRDHLLEGTFNQVMAYSRKELQRNKLYVTFVGEEGLDYSGPSREFFFLLSQELFNPYYGLFEYSANDTYTVQISPMSAFVENHLEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRLPCDLSDLEYLDEEFHQSLQWMKDNNITDILDLTFTVNEEVFGQVTERELKSGGANTQVTEKNKKEYIERMVKWRVERGVVQQTEALVRGFYEVVDSRLVSVFDARELELVIAGTAEIDLNDWRNNTEYRGGYHDGHLVIRWFWAAVERFNNEQRLRLLQFVTGTSSVPYEGFAALRGSNGLRRFCIEKWGKITSLPRAHTCFNRLDLPPYPSYSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_015075294.1
Organism	Acinonyx jubatus(cheetah)
Definition	Acinonyx jubatus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015075294.1

ORF Insert Sequence:

ATGCTGCTGC ACCTCTGTAG TGTGAAGAAT CTGTACCAGA ACAGGTTTTT AGGCCTGGCC 
GCCATGGCAT CCCCATCTAG AAACTCCCAG AATCGCCGCC GGTGCAAGGA GCCACTCCGT 
CACAGCTACA ACCCTGGCCA GTTCCACAGC ATGGCCATCA GGACCGGCCC CCACGGCACC 
GTCACCATAC CCCGCTCCAC CAGCGACACC GACCTGGTCA CCTCAGACAG CCGCTCCACA 
CTTATGGTCA GCAGCTCCTA CTACTCCATA GGACACTCTC AGGACCTGGT GATCCACTGG 
GACATCAAGG AGGAAGTGGA TGCTGGAGAC TGGATTGGCA TGTATCTCAT TGATGAGGTC 
TTGTCAGAAA ACTTTTTGGA CTATAAGAAC CGTGGAGTCA ATGGTTCTCA TCGGGGCCAG 
ATCATCTGGA AGATTGATGC CAGCTCTTAC TTTGTGGAAC CCGAAACTAA GATCTGCTTC 
AAATACTACC ATGGCGTGAG TGGAGCCCTG CGAGCCACCA CCCCCAGTGT CACGGTCAAA 
AACTCTGCAG CTCCTATTTT TAAAAGCATT GGCTCTGATG AGACCGTCCA AGGGCAAGGA 
AGTCGGAGGC TGATCAGCTT TTCTCTCTCA GATTTCCAGG CCATGGGGCT GAAGAAAGGG 
ATGTTTTTCA ACCCAGACCC TTACCTGAAG ATTTCCATTC AGCCTGGAAA GCACAGCATC 
TTCCCTGCCC TCCCACACCA TGGACAGGAG AGGAGATCCA AGATCATAGG CAACACTGTG 
AACCCCATCT GGCAGGCCGA GCAATTCAGC TTTGTGTCCT TGCCCACTGA TGTACTGGAA 
ATTGAGGTGA AAGACAAGTT TGCTAAGAGC CGCCCCATCA TCAAGCGCTT CTTGGGAAAG 
CTGTCGATGC CCGTTCAAAG ACTCCTGGAG AGACACGCCA TAGGGGATAG GGTGGTCAGC 
TATACACTTG GCCGCAGGCT TCCAACAGAT CATGTGAGTG GACAGCTGCA ATTCCGATTT 
GAGATCACTT CTTCCATCCA CCCAGATGAT GAGGAGATGT CCCTCAGCGC CGAGCCTGAG 
TCCGCGGACA TTCAGGACAG CCCCATGAAC AGCCTGTTGG CCAGCAGCCG TGGGGAGCCT 
CCCGGCGAGG CACCGGAGCT CCCCAGGGGC TCCACGCCCG ACAGCCACAC CCGCTTCTCC 
TCCGTGGACA GCGCCAAGAT CTCTGAGAGC ACCGTCTTCT CGTCGCAAGA CGATGAGGAG 
GAGGAGAACA GTGCGTTCGA ATCTGTGCCC GACTCGGTAC AGAGCCCGGA GCTGGACCCG 
GAGTCGGCAA ATGGCGCCGG GCCGTGGCAC GACGAGCTGG CCGCCCCCGC CGCCAGCGTG 
GCAAGAACCG TGGAAGGTCT GGAATCCCCC GTGGCAGGGC CAAGCAATCG GAGAGAAGGT 
GAATGTCCTA TACTCCATAA TTCCCAGCCA GTAAGCCAGC TTCCTTCCTT GAGGCCTGAA 
CATCATCACT ACCCAACAAT CGATGAGCCT CTTCCACCAA ACTGGGAAGC TCGCATTGAC 
AGCCACGGGC GGGTCTTTTA TGTGGACCAC GTGAACCGCA CGACCACCTG GCAGCGGCCG 
ACAGCAGCAG CCACCCCCGA CGGGATGCGG AGATCGGGGT CCATCCAGCA GATGGAGCAA 
CTCAACAGGC GGTATCAAAA TATTCAGCGA ACCATTGCAA CAGAGAGACC AGAAGAAGAT 
TCTGGAGGCC AAAGTTGTGA GCAGGTCCCA ACAGGAGGCA GTGGAGGCGG TGGGAGCGAC 
TCAGAGGCTG ACTCTTCTCA ATCAAGCTTA GATCTGAGGA GAGAAGGGTC ACTTTCTCCA 
GTGAACTCAC AAAAAATCAC CTTGTTGCTG CAGTCCCCAG CAGTGAAGTT CATTACCAAT 
CCCGAGTTTT TCACTGTACT ACACGCCAAT TATGGTGCCT ACCGAGTCTT CACCAGTAGC 
ACCTGCTTAA AGCACATGAT TCTGAAAGTC CGGCGGGACG CTCGCAATTT CGAACGCTAC 
CAGCACAACC GTGACTTGGT GAATTTCATC AACATGTTTG CAGACACACG GCTGGAATTG 
CCCCGGGGCT GGGAGATCAA AACGGACCAG CAGGGAAAGT CTTTTTTCGT GGACCACAAT 
AGTCGAGCCA CCACTTTCAT TGACCCCCGA ATCCCTCTTC AGAACGGTCG TCTTCCCAAT 
CACCTGACAC ATCGACAGCA CCTCCAGAGG CTCCGGAGTT ACAGCGCTGG AGAGGCTTCA 
GAAGTCTCTC GAAACAGAGG AGCCTCTTTA CTGGCCAGGC CAGGACACAG CCTGGTAGCT 
GCTATTCGAA GCCAACATCA ACATGAGTCA TTGCCACTAG CATATAATGA CAAGATTGTG 
GCATTTCTCC GCCAGCCAAA CATTTTTGAA ATGCTGCAAG AGCGTCAGCC AAGCCTGGCG 
AGAAACCACA CACTCAGGGA GAAAATCCAT TACATTCGGA CTGAGGGTAA TCATGGGCTT 
GAAAAGTTGT CCTGTGATGC AGATCTAGTC ATTTTGCTGA GTCTCTTTGA AGAAGAGATT 
ATGTCCTACG TCCCACTGCA GACTGCCTTT CATCCCGGGT ACAGCTTCTC TCCCCGCTGT 
TCACCCTGTT CCTCACCTCA GAACTCCCCA GGTTTACAGA GAGCCAGTGC AAGAGCCCCT 
TCACCCTACC GGAGAGACTT CGAGGCCAAG CTCCGCAATT TCTACCGAAA ACTGGAAGCC 
AAAGGATTTG GTCAGGGTCC AGGGAAAATT AAGCTCATCA TTCGTCGGGA CCACTTGTTA 
GAAGGAACCT TCAATCAGGT GATGGCCTAT TCCCGGAAGG AGCTTCAGCG AAACAAACTC 
TACGTTACCT TTGTTGGAGA GGAGGGTCTG GACTACAGTG GTCCTTCGCG AGAGTTCTTC 
TTCCTTTTGT CTCAGGAGCT CTTCAACCCT TACTACGGAC TCTTTGAGTA CTCTGCAAAT 
GATACGTACA CGGTGCAGAT CAGCCCCATG TCGGCATTTG TGGAAAACCA TCTTGAATGG 
TTCAGGTTTA GTGGTCGGAT CCTAGGCCTG GCTTTGATCC ATCAGTACCT TCTTGATGCC 
TTCTTCACAA GGCCCTTCTA TAAGGCACTC CTGAGACTGC CCTGTGATTT AAGTGATCTG 
GAATATCTGG ATGAGGAATT TCACCAGAGT TTGCAATGGA TGAAGGACAA CAACATCACA 
GATATCCTAG ACCTCACTTT CACTGTTAAC GAAGAAGTTT TTGGACAGGT AACGGAAAGG 
GAGTTGAAAT CTGGAGGAGC CAACACGCAG GTGACGGAGA AGAACAAGAA GGAGTACATT 
GAGAGGATGG TGAAGTGGCG GGTGGAGCGC GGTGTGGTGC AGCAGACCGA GGCCCTGGTG 
CGGGGCTTCT ATGAGGTGGT AGACTCGAGG CTTGTCTCTG TGTTCGACGC CAGGGAGCTG 
GAGCTGGTGA TAGCAGGCAC GGCGGAAATC GACCTGAATG ACTGGCGGAA TAACACAGAG 
TACCGGGGAG GTTATCATGA CGGGCATCTT GTGATCCGCT GGTTCTGGGC CGCCGTGGAG 
CGCTTCAATA ATGAGCAGAG ACTGAGATTA CTTCAGTTCG TCACGGGAAC GTCAAGCGTG 
CCCTACGAAG GCTTCGCAGC CCTTCGAGGA AGCAATGGGC TTCGGCGTTT CTGCATAGAG 
AAGTGGGGGA AGATTACGTC TCTCCCCAGG GCACACACAT GCTTCAACCG ACTGGATCTT 
CCACCTTATC CCTCATACTC CATGTTATAT GAAAAGCTGC TAACAGCAGT AGAAGAAACC 
AGCACCTTTG GACTTGAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HECW1 ( XM_027071591.1 ) cDNA ORF clone, Acinonyx jubatus(cheetah) -> XP_026927392.1 Acinonyx jubatus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OAc228957
Clone ID Related Accession (Same CDS sequence)	XM_027071591.1
Accession Version	XM_027071591.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4905bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-12
Organism	Acinonyx jubatus(cheetah)
Product	E3 ubiquitin-protein ligase HECW1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_020834755.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Acinonyx jubatus Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCTACC TGAAAAGGCC AGACTTTAAA GACCCAGGCA GTGTCGTGGT CCAAGGCGTC 
TCAAAGCAGG TGGCCAGATC TGCGTTTCTC ATCAGCGGAC TCGCTCCGGC TGTGGCTATT 
ACGAATCTGT ACCAGAACAG GTTTTTAGGC CTGGCCGCCA TGGCATCCCC ATCTAGAAAC 
TCCCAGAATC GCCGCCGGTG CAAGGAGCCA CTCCGTCACA GCTACAACCC TGGCCAGTTC 
CACAGCATGG CCATCAGGAC CGGCCCCCAC GGCACCGTCA CCATACCCCG CTCCACCAGC 
GACACCGACC TGGTCACCTC AGACAGCCGC TCCACACTTA TGGTCAGCAG CTCCTACTAC 
TCCATAGGAC ACTCTCAGGA CCTGGTGATC CACTGGGACA TCAAGGAGGA AGTGGATGCT 
GGAGACTGGA TTGGCATGTA TCTCATTGAT GAGGTCTTGT CAGAAAACTT TTTGGACTAT 
AAGAACCGTG GAGTCAATGG TTCTCATCGG GGCCAGATCA TCTGGAAGAT TGATGCCAGC 
TCTTACTTTG TGGAACCCGA AACTAAGATC TGCTTCAAAT ACTACCATGG CGTGAGTGGA 
GCCCTGCGAG CCACCACCCC CAGTGTCACG GTCAAAAACT CTGCAGCTCC TATTTTTAAA 
AGCATTGGCT CTGATGAGAC CGTCCAAGGG CAAGGAAGTC GGAGGCTGAT CAGCTTTTCT 
CTCTCAGATT TCCAGGCCAT GGGGCTGAAG AAAGGGATGT TTTTCAACCC AGACCCTTAC 
CTGAAGATTT CCATTCAGCC TGGAAAGCAC AGCATCTTCC CTGCCCTCCC ACACCATGGA 
CAGGAGAGGA GATCCAAGAT CATAGGCAAC ACTGTGAACC CCATCTGGCA GGCCGAGCAA 
TTCAGCTTTG TGTCCTTGCC CACTGATGTA CTGGAAATTG AGGTGAAAGA CAAGTTTGCT 
AAGAGCCGCC CCATCATCAA GCGCTTCTTG GGAAAGCTGT CGATGCCCGT TCAAAGACTC 
CTGGAGAGAC ACGCCATAGG GGATAGGGTG GTCAGCTATA CACTTGGCCG CAGGCTTCCA 
ACAGATCATG TGAGTGGACA GCTGCAATTC CGATTTGAGA TCACTTCTTC CATCCACCCA 
GATGATGAGG AGATGTCCCT CAGCGCCGAG CCTGAGTCCG CGGACATTCA GGACAGCCCC 
ATGAACAGCC TGTTGGCCAG CAGCCGTGGG GAGCCTCCCG GCGAGGCACC GGAGCTCCCC 
AGGGGCTCCA AGCCCGAGCC GCCGAGCCAG GGCAGCGCCG GGGACGCTGC CGGGAACGGG 
AGCCTGGAGC TGGCCGAGCC GGTTGAGGAA GCGGCAGGTA CCAACGAGGC AGGAGACGAC 
GGCGCGGGCT CTGTGGGACC TGACGGGGCT GGGGAGCTTC TGGGCCCGGG GCGTGAGGAC 
GCCGAGCCCG CCCTGAGTGC GGAAGAACTG GCGGAGCGGC TGGAGCTGGG TGGGGAGGTG 
CCCCCACCGG GGCTGCTGGA GGACAGCGAA GGCCCGGGCG GCACCTGGGA GCCCGAGGCA 
GAGGAAGAGG CCACAGAGAG CCGGGCGGGG GGAGAGGAGG AGAAGGAGCA GGCGGAGGAG 
GAGGAGGAGG GCGACGTGTC CGCCCTGGGG CAGGGGGAGG ACAGGCTGCA GCTGCGGGCC 
TCGGTGAAGA GGAAAAGCAG GCCGTGCTCC TTGCCGGTGT CCGAGCTGGA GACGGTGATC 
GCGTCCGCCT GCGGAGACCC CGAGACCCCG CGGACACACT ACATCCGCAT CCACACCCTG 
CTGCACAGCA TGCCCTCCGC CCAGGGCGGC GGCGCCGCGA GCGACGACGG CGCCGAGGAG 
GAGGCCACCC TCCAGGACGC CTCGGAGAAG GACGGGCTCA GCGAGGTGGA CACGCTAGCT 
GCCGCGCCGC CTCCCCTCGA AGAGGATGGG GAGGAACTCG AGGGGGCTAC TCCAGGCACG 
GCGACCCCCG GCCACTCCGG CCTGACCAAC GGCCTGGGCC CGGACGGCGA CACGCTCCCC 
AGCACAGGGA GTGAGAGCGA CTCGAGTCCC CGGCAGGGTG GGGACCACAG CTGCGAGGGC 
TGTGACACGT CCTGCTGCAG CCCCTCCTGC TACAGCTCCT CGTGCTACAG CACGTCCTGC 
TACAGCAGCT CCTGCTACAG CGCCTCCTGC TATAGCCCCT CCTGTTACAA CGGCAGCAGG 
TTCGCCAGCC ACACCCGCTT CTCCTCCGTG GACAGCGCCA AGATCTCTGA GAGCACCGTC 
TTCTCGTCGC AAGACGATGA GGAGGAGGAG AACAGTGCGT TCGAATCTGT GCCCGACTCG 
GTACAGAGCC CGGAGCTGGA CCCGGAGTCG GCAAATGGCG CCGGGCCGTG GCACGACGAG 
CTGGCCGCCC CCGCCGCCAG CGTGGCAAGA ACCGTGGAAG GTCTGGAATC CCCCGTGGCA 
GGGCCAAGCA ATCGGAGAGA AGGTGAATGT CCTATACTCC ATAATTCCCA GCCAGTAAGC 
CAGCTTCCTT CCTTGAGGCC TGAACATCAT CACTACCCAA CAATCGATGA GCCTCTTCCA 
CCAAACTGGG AAGCTCGCAT TGACAGCCAC GGGCGGGTCT TTTATGTGGA CCACGTGAAC 
CGCACGACCA CCTGGCAGCG GCCGACAGCA GCAGCCACCC CCGACGGGAT GCGGAGATCG 
GGGTCCATCC AGCAGATGGA GCAACTCAAC AGGCGGTATC AAAATATTCA GCGAACCATT 
GCAACAGAGA GACCAGAAGA AGATTCTGGA GGCCAAAGTT GTGAGCAGGT CCCAACAGGA 
GGCAGTGGAG GCGGTGGGAG CGACTCAGAG GCTGACTCTT CTCAATCAAG CTTAGATCTG 
AGGAGAGAAG GGTCACTTTC TCCAGTGAAC TCACAAAAAA TCACCTTGTT GCTGCAGTCC 
CCAGCAGTGA AGTTCATTAC CAATCCCGAG TTTTTCACTG TACTACACGC CAATTATGGT 
GCCTACCGAG TCTTCACCAG TAGCACCTGC TTAAAGCACA TGATTCTGAA AGTCCGGCGG 
GACGCTCGCA ATTTCGAACG CTACCAGCAC AACCGTGACT TGGTGAATTT CATCAACATG 
TTTGCAGACA CACGGCTGGA ATTGCCCCGG GGCTGGGAGA TCAAAACGGA CCAGCAGGGA 
AAGTCTTTTT TCGTGGACCA CAATAGTCGA GCCACCACTT TCATTGACCC CCGAATCCCT 
CTTCAGAACG GTCGTCTTCC CAATCACCTG ACACATCGAC AGCACCTCCA GAGGCTCCGG 
AGTTACAGCG CTGGAGAGGC TTCAGAAGTC TCTCGAAACA GAGGAGCCTC TTTACTGGCC 
AGGCCAGGAC ACAGCCTGGT AGCTGCTATT CGAAGCCAAC ATCAACATGA GTCATTGCCA 
CTAGCATATA ATGACAAGAT TGTGGCATTT CTCCGCCAGC CAAACATTTT TGAAATGCTG 
CAAGAGCGTC AGCCAAGCCT GGCGAGAAAC CACACACTCA GGGAGAAAAT CCATTACATT 
CGGACTGAGG GTAATCATGG GCTTGAAAAG TTGTCCTGTG ATGCAGATCT AGTCATTTTG 
CTGAGTCTCT TTGAAGAAGA GATTATGTCC TACGTCCCAC TGCAGACTGC CTTTCATCCC 
GGGTACAGCT TCTCTCCCCG CTGTTCACCC TGTTCCTCAC CTCAGAACTC CCCAGGTTTA 
CAGAGAGCCA GTGCAAGAGC CCCTTCACCC TACCGGAGAG ACTTCGAGGC CAAGCTCCGC 
AATTTCTACC GAAAACTGGA AGCCAAAGGA TTTGGTCAGG GTCCAGGGAA AATTAAGCTC 
ATCATTCGTC GGGACCACTT GTTAGAAGGA ACCTTCAATC AGGTGATGGC CTATTCCCGG 
AAGGAGCTTC AGCGAAACAA ACTCTACGTT ACCTTTGTTG GAGAGGAGGG TCTGGACTAC 
AGTGGTCCTT CGCGAGAGTT CTTCTTCCTT TTGTCTCAGG AGCTCTTCAA CCCTTACTAC 
GGACTCTTTG AGTACTCTGC AAATGATACG TACACGGTGC AGATCAGCCC CATGTCGGCA 
TTTGTGGAAA ACCATCTTGA ATGGTTCAGG TTTAGTGGTC GGATCCTAGG CCTGGCTTTG 
ATCCATCAGT ACCTTCTTGA TGCCTTCTTC ACAAGGCCCT TCTATAAGGC ACTCCTGAGA 
CTGCCCTGTG ATTTAAGTGA TCTGGAATAT CTGGATGAGG AATTTCACCA GAGTTTGCAA 
TGGATGAAGG ACAACAACAT CACAGATATC CTAGACCTCA CTTTCACTGT TAACGAAGAA 
GTTTTTGGAC AGGTAACGGA AAGGGAGTTG AAATCTGGAG GAGCCAACAC GCAGGTGACG 
GAGAAGAACA AGAAGGAGTA CATCGAGAGG ATGGTGAAGT GGCGGGTGGA GCGCGGTGTG 
GTGCAGCAGA CCGAGGCCCT GGTGCGGGGC TTCTATGAGG TGGTAGACTC GAGGCTTGTC 
TCTGTGTTCG ACGCCAGGGA GCTGGAGCTG GTGATAGCAG GCACGGCGGA AATCGACCTG 
AATGACTGGC GGAATAACAC AGAGTACCGG GGAGGTTATC ATGACGGGCA TCTTGTGATC 
CGCTGGTTCT GGGCCGCCGT GGAGCGCTTC AATAATGAGC AGAGACTGAG ATTACTTCAG 
TTCGTCACGG GAACGTCAAG CGTGCCCTAC GAAGGCTTCG CAGCCCTTCG AGGAAGCAAT 
GGGCTTCGGC GTTTCTGCAT AGAGAAGTGG GGGAAGATTA CGTCTCTCCC CAGGGCACAC 
ACATGCTTCA ACCGACTGGA TCTTCCACCT TATCCCTCAT ACTCCATGTT ATATGAAAAG 
CTGCTAACAG CAGTAGAAGA AACCAGCACC TTTGGACTTG AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_026927392.1
CDS	50..4954
Translation	MPYLKRPDFKDPGSVVVQGVSKQVARSAFLISGLAPAVAITNLYQNRFLGLAAMASPSRNSQNRRRCKEPLRHSYNPGQFHSMAIRTGPHGTVTIPRSTSDTDLVTSDSRSTLMVSSSYYSIGHSQDLVIHWDIKEEVDAGDWIGMYLIDEVLSENFLDYKNRGVNGSHRGQIIWKIDASSYFVEPETKICFKYYHGVSGALRATTPSVTVKNSAAPIFKSIGSDETVQGQGSRRLISFSLSDFQAMGLKKGMFFNPDPYLKISIQPGKHSIFPALPHHGQERRSKIIGNTVNPIWQAEQFSFVSLPTDVLEIEVKDKFAKSRPIIKRFLGKLSMPVQRLLERHAIGDRVVSYTLGRRLPTDHVSGQLQFRFEITSSIHPDDEEMSLSAEPESADIQDSPMNSLLASSRGEPPGEAPELPRGSKPEPPSQGSAGDAAGNGSLELAEPVEEAAGTNEAGDDGAGSVGPDGAGELLGPGREDAEPALSAEELAERLELGGEVPPPGLLEDSEGPGGTWEPEAEEEATESRAGGEEEKEQAEEEEEGDVSALGQGEDRLQLRASVKRKSRPCSLPVSELETVIASACGDPETPRTHYIRIHTLLHSMPSAQGGGAASDDGAEEEATLQDASEKDGLSEVDTLAAAPPPLEEDGEELEGATPGTATPGHSGLTNGLGPDGDTLPSTGSESDSSPRQGGDHSCEGCDTSCCSPSCYSSSCYSTSCYSSSCYSASCYSPSCYNGSRFASHTRFSSVDSAKISESTVFSSQDDEEEENSAFESVPDSVQSPELDPESANGAGPWHDELAAPAASVARTVEGLESPVAGPSNRREGECPILHNSQPVSQLPSLRPEHHHYPTIDEPLPPNWEARIDSHGRVFYVDHVNRTTTWQRPTAAATPDGMRRSGSIQQMEQLNRRYQNIQRTIATERPEEDSGGQSCEQVPTGGSGGGGSDSEADSSQSSLDLRREGSLSPVNSQKITLLLQSPAVKFITNPEFFTVLHANYGAYRVFTSSTCLKHMILKVRRDARNFERYQHNRDLVNFINMFADTRLELPRGWEIKTDQQGKSFFVDHNSRATTFIDPRIPLQNGRLPNHLTHRQHLQRLRSYSAGEASEVSRNRGASLLARPGHSLVAAIRSQHQHESLPLAYNDKIVAFLRQPNIFEMLQERQPSLARNHTLREKIHYIRTEGNHGLEKLSCDADLVILLSLFEEEIMSYVPLQTAFHPGYSFSPRCSPCSSPQNSPGLQRASARAPSPYRRDFEAKLRNFYRKLEAKGFGQGPGKIKLIIRRDHLLEGTFNQVMAYSRKELQRNKLYVTFVGEEGLDYSGPSREFFFLLSQELFNPYYGLFEYSANDTYTVQISPMSAFVENHLEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRLPCDLSDLEYLDEEFHQSLQWMKDNNITDILDLTFTVNEEVFGQVTERELKSGGANTQVTEKNKKEYIERMVKWRVERGVVQQTEALVRGFYEVVDSRLVSVFDARELELVIAGTAEIDLNDWRNNTEYRGGYHDGHLVIRWFWAAVERFNNEQRLRLLQFVTGTSSVPYEGFAALRGSNGLRRFCIEKWGKITSLPRAHTCFNRLDLPPYPSYSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_027071591.1
Organism	Acinonyx jubatus(cheetah)
Definition	Acinonyx jubatus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_027071591.1

ORF Insert Sequence:

ATGCCCTACC TGAAAAGGCC AGACTTTAAA GACCCAGGCA GTGTCGTGGT CCAAGGCGTC 
TCAAAGCAGG TGGCCAGATC TGCGTTTCTC ATCAGCGGAC TCGCTCCGGC TGTGGCTATT 
ACGAATCTGT ACCAGAACAG GTTTTTAGGC CTGGCCGCCA TGGCATCCCC ATCTAGAAAC 
TCCCAGAATC GCCGCCGGTG CAAGGAGCCA CTCCGTCACA GCTACAACCC TGGCCAGTTC 
CACAGCATGG CCATCAGGAC CGGCCCCCAC GGCACCGTCA CCATACCCCG CTCCACCAGC 
GACACCGACC TGGTCACCTC AGACAGCCGC TCCACACTTA TGGTCAGCAG CTCCTACTAC 
TCCATAGGAC ACTCTCAGGA CCTGGTGATC CACTGGGACA TCAAGGAGGA AGTGGATGCT 
GGAGACTGGA TTGGCATGTA TCTCATTGAT GAGGTCTTGT CAGAAAACTT TTTGGACTAT 
AAGAACCGTG GAGTCAATGG TTCTCATCGG GGCCAGATCA TCTGGAAGAT TGATGCCAGC 
TCTTACTTTG TGGAACCCGA AACTAAGATC TGCTTCAAAT ACTACCATGG CGTGAGTGGA 
GCCCTGCGAG CCACCACCCC CAGTGTCACG GTCAAAAACT CTGCAGCTCC TATTTTTAAA 
AGCATTGGCT CTGATGAGAC CGTCCAAGGG CAAGGAAGTC GGAGGCTGAT CAGCTTTTCT 
CTCTCAGATT TCCAGGCCAT GGGGCTGAAG AAAGGGATGT TTTTCAACCC AGACCCTTAC 
CTGAAGATTT CCATTCAGCC TGGAAAGCAC AGCATCTTCC CTGCCCTCCC ACACCATGGA 
CAGGAGAGGA GATCCAAGAT CATAGGCAAC ACTGTGAACC CCATCTGGCA GGCCGAGCAA 
TTCAGCTTTG TGTCCTTGCC CACTGATGTA CTGGAAATTG AGGTGAAAGA CAAGTTTGCT 
AAGAGCCGCC CCATCATCAA GCGCTTCTTG GGAAAGCTGT CGATGCCCGT TCAAAGACTC 
CTGGAGAGAC ACGCCATAGG GGATAGGGTG GTCAGCTATA CACTTGGCCG CAGGCTTCCA 
ACAGATCATG TGAGTGGACA GCTGCAATTC CGATTTGAGA TCACTTCTTC CATCCACCCA 
GATGATGAGG AGATGTCCCT CAGCGCCGAG CCTGAGTCCG CGGACATTCA GGACAGCCCC 
ATGAACAGCC TGTTGGCCAG CAGCCGTGGG GAGCCTCCCG GCGAGGCACC GGAGCTCCCC 
AGGGGCTCCA AGCCCGAGCC GCCGAGCCAG GGCAGCGCCG GGGACGCTGC CGGGAACGGG 
AGCCTGGAGC TGGCCGAGCC GGTTGAGGAA GCGGCAGGTA CCAACGAGGC AGGAGACGAC 
GGCGCGGGCT CTGTGGGACC TGACGGGGCT GGGGAGCTTC TGGGCCCGGG GCGTGAGGAC 
GCCGAGCCCG CCCTGAGTGC GGAAGAACTG GCGGAGCGGC TGGAGCTGGG TGGGGAGGTG 
CCCCCACCGG GGCTGCTGGA GGACAGCGAA GGCCCGGGCG GCACCTGGGA GCCCGAGGCA 
GAGGAAGAGG CCACAGAGAG CCGGGCGGGG GGAGAGGAGG AGAAGGAGCA GGCGGAGGAG 
GAGGAGGAGG GCGACGTGTC CGCCCTGGGG CAGGGGGAGG ACAGGCTGCA GCTGCGGGCC 
TCGGTGAAGA GGAAAAGCAG GCCGTGCTCC TTGCCGGTGT CCGAGCTGGA GACGGTGATC 
GCGTCCGCCT GCGGAGACCC CGAGACCCCG CGGACACACT ACATCCGCAT CCACACCCTG 
CTGCACAGCA TGCCCTCCGC CCAGGGCGGC GGCGCCGCGA GCGACGACGG CGCCGAGGAG 
GAGGCCACCC TCCAGGACGC CTCGGAGAAG GACGGGCTCA GCGAGGTGGA CACGCTAGCT 
GCCGCGCCGC CTCCCCTCGA AGAGGATGGG GAGGAACTCG AGGGGGCTAC TCCAGGCACG 
GCGACCCCCG GCCACTCCGG CCTGACCAAC GGCCTGGGCC CGGACGGCGA CACGCTCCCC 
AGCACAGGGA GTGAGAGCGA CTCGAGTCCC CGGCAGGGTG GGGACCACAG CTGCGAGGGC 
TGTGACACGT CCTGCTGCAG CCCCTCCTGC TACAGCTCCT CGTGCTACAG CACGTCCTGC 
TACAGCAGCT CCTGCTACAG CGCCTCCTGC TATAGCCCCT CCTGTTACAA CGGCAGCAGG 
TTCGCCAGCC ACACCCGCTT CTCCTCCGTG GACAGCGCCA AGATCTCTGA GAGCACCGTC 
TTCTCGTCGC AAGACGATGA GGAGGAGGAG AACAGTGCGT TCGAATCTGT GCCCGACTCG 
GTACAGAGCC CGGAGCTGGA CCCGGAGTCG GCAAATGGCG CCGGGCCGTG GCACGACGAG 
CTGGCCGCCC CCGCCGCCAG CGTGGCAAGA ACCGTGGAAG GTCTGGAATC CCCCGTGGCA 
GGGCCAAGCA ATCGGAGAGA AGGTGAATGT CCTATACTCC ATAATTCCCA GCCAGTAAGC 
CAGCTTCCTT CCTTGAGGCC TGAACATCAT CACTACCCAA CAATCGATGA GCCTCTTCCA 
CCAAACTGGG AAGCTCGCAT TGACAGCCAC GGGCGGGTCT TTTATGTGGA CCACGTGAAC 
CGCACGACCA CCTGGCAGCG GCCGACAGCA GCAGCCACCC CCGACGGGAT GCGGAGATCG 
GGGTCCATCC AGCAGATGGA GCAACTCAAC AGGCGGTATC AAAATATTCA GCGAACCATT 
GCAACAGAGA GACCAGAAGA AGATTCTGGA GGCCAAAGTT GTGAGCAGGT CCCAACAGGA 
GGCAGTGGAG GCGGTGGGAG CGACTCAGAG GCTGACTCTT CTCAATCAAG CTTAGATCTG 
AGGAGAGAAG GGTCACTTTC TCCAGTGAAC TCACAAAAAA TCACCTTGTT GCTGCAGTCC 
CCAGCAGTGA AGTTCATTAC CAATCCCGAG TTTTTCACTG TACTACACGC CAATTATGGT 
GCCTACCGAG TCTTCACCAG TAGCACCTGC TTAAAGCACA TGATTCTGAA AGTCCGGCGG 
GACGCTCGCA ATTTCGAACG CTACCAGCAC AACCGTGACT TGGTGAATTT CATCAACATG 
TTTGCAGACA CACGGCTGGA ATTGCCCCGG GGCTGGGAGA TCAAAACGGA CCAGCAGGGA 
AAGTCTTTTT TCGTGGACCA CAATAGTCGA GCCACCACTT TCATTGACCC CCGAATCCCT 
CTTCAGAACG GTCGTCTTCC CAATCACCTG ACACATCGAC AGCACCTCCA GAGGCTCCGG 
AGTTACAGCG CTGGAGAGGC TTCAGAAGTC TCTCGAAACA GAGGAGCCTC TTTACTGGCC 
AGGCCAGGAC ACAGCCTGGT AGCTGCTATT CGAAGCCAAC ATCAACATGA GTCATTGCCA 
CTAGCATATA ATGACAAGAT TGTGGCATTT CTCCGCCAGC CAAACATTTT TGAAATGCTG 
CAAGAGCGTC AGCCAAGCCT GGCGAGAAAC CACACACTCA GGGAGAAAAT CCATTACATT 
CGGACTGAGG GTAATCATGG GCTTGAAAAG TTGTCCTGTG ATGCAGATCT AGTCATTTTG 
CTGAGTCTCT TTGAAGAAGA GATTATGTCC TACGTCCCAC TGCAGACTGC CTTTCATCCC 
GGGTACAGCT TCTCTCCCCG CTGTTCACCC TGTTCCTCAC CTCAGAACTC CCCAGGTTTA 
CAGAGAGCCA GTGCAAGAGC CCCTTCACCC TACCGGAGAG ACTTCGAGGC CAAGCTCCGC 
AATTTCTACC GAAAACTGGA AGCCAAAGGA TTTGGTCAGG GTCCAGGGAA AATTAAGCTC 
ATCATTCGTC GGGACCACTT GTTAGAAGGA ACCTTCAATC AGGTGATGGC CTATTCCCGG 
AAGGAGCTTC AGCGAAACAA ACTCTACGTT ACCTTTGTTG GAGAGGAGGG TCTGGACTAC 
AGTGGTCCTT CGCGAGAGTT CTTCTTCCTT TTGTCTCAGG AGCTCTTCAA CCCTTACTAC 
GGACTCTTTG AGTACTCTGC AAATGATACG TACACGGTGC AGATCAGCCC CATGTCGGCA 
TTTGTGGAAA ACCATCTTGA ATGGTTCAGG TTTAGTGGTC GGATCCTAGG CCTGGCTTTG 
ATCCATCAGT ACCTTCTTGA TGCCTTCTTC ACAAGGCCCT TCTATAAGGC ACTCCTGAGA 
CTGCCCTGTG ATTTAAGTGA TCTGGAATAT CTGGATGAGG AATTTCACCA GAGTTTGCAA 
TGGATGAAGG ACAACAACAT CACAGATATC CTAGACCTCA CTTTCACTGT TAACGAAGAA 
GTTTTTGGAC AGGTAACGGA AAGGGAGTTG AAATCTGGAG GAGCCAACAC GCAGGTGACG 
GAGAAGAACA AGAAGGAGTA CATCGAGAGG ATGGTGAAGT GGCGGGTGGA GCGCGGTGTG 
GTGCAGCAGA CCGAGGCCCT GGTGCGGGGC TTCTATGAGG TGGTAGACTC GAGGCTTGTC 
TCTGTGTTCG ACGCCAGGGA GCTGGAGCTG GTGATAGCAG GCACGGCGGA AATCGACCTG 
AATGACTGGC GGAATAACAC AGAGTACCGG GGAGGTTATC ATGACGGGCA TCTTGTGATC 
CGCTGGTTCT GGGCCGCCGT GGAGCGCTTC AATAATGAGC AGAGACTGAG ATTACTTCAG 
TTCGTCACGG GAACGTCAAG CGTGCCCTAC GAAGGCTTCG CAGCCCTTCG AGGAAGCAAT 
GGGCTTCGGC GTTTCTGCAT AGAGAAGTGG GGGAAGATTA CGTCTCTCCC CAGGGCACAC 
ACATGCTTCA ACCGACTGGA TCTTCCACCT TATCCCTCAT ACTCCATGTT ATATGAAAAG 
CTGCTAACAG CAGTAGAAGA AACCAGCACC TTTGGACTTG AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HECW1 ( XM_027071593.1 ) cDNA ORF clone, Acinonyx jubatus(cheetah) -> XP_026927394.1 Acinonyx jubatus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X2, mRNA.

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CloneID	OAc228958
Clone ID Related Accession (Same CDS sequence)	XM_027071593.1
Accession Version	XM_027071593.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4809bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-12
Organism	Acinonyx jubatus(cheetah)
Product	E3 ubiquitin-protein ligase HECW1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_020834755.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Acinonyx jubatus Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCTGC ACCTCTGTAG TGTGAAGAAT CTGTACCAGA ACAGGTTTTT AGGCCTGGCC 
GCCATGGCAT CCCCATCTAG AAACTCCCAG AATCGCCGCC GGTGCAAGGA GCCACTCCGT 
CACAGCTACA ACCCTGGCCA GTTCCACAGC ATGGCCATCA GGACCGGCCC CCACGGCACC 
GTCACCATAC CCCGCTCCAC CAGCGACACC GACCTGGTCA CCTCAGACAG CCGCTCCACA 
CTTATGGTCA GCAGCTCCTA CTACTCCATA GGACACTCTC AGGACCTGGT GATCCACTGG 
GACATCAAGG AGGAAGTGGA TGCTGGAGAC TGGATTGGCA TGTATCTCAT TGATGAGGTC 
TTGTCAGAAA ACTTTTTGGA CTATAAGAAC CGTGGAGTCA ATGGTTCTCA TCGGGGCCAG 
ATCATCTGGA AGATTGATGC CAGCTCTTAC TTTGTGGAAC CCGAAACTAA GATCTGCTTC 
AAATACTACC ATGGCGTGAG TGGAGCCCTG CGAGCCACCA CCCCCAGTGT CACGGTCAAA 
AACTCTGCAG CTCCTATTTT TAAAAGCATT GGCTCTGATG AGACCGTCCA AGGGCAAGGA 
AGTCGGAGGC TGATCAGCTT TTCTCTCTCA GATTTCCAGG CCATGGGGCT GAAGAAAGGG 
ATGTTTTTCA ACCCAGACCC TTACCTGAAG ATTTCCATTC AGCCTGGAAA GCACAGCATC 
TTCCCTGCCC TCCCACACCA TGGACAGGAG AGGAGATCCA AGATCATAGG CAACACTGTG 
AACCCCATCT GGCAGGCCGA GCAATTCAGC TTTGTGTCCT TGCCCACTGA TGTACTGGAA 
ATTGAGGTGA AAGACAAGTT TGCTAAGAGC CGCCCCATCA TCAAGCGCTT CTTGGGAAAG 
CTGTCGATGC CCGTTCAAAG ACTCCTGGAG AGACACGCCA TAGGGGATAG GGTGGTCAGC 
TATACACTTG GCCGCAGGCT TCCAACAGAT CATGTGAGTG GACAGCTGCA ATTCCGATTT 
GAGATCACTT CTTCCATCCA CCCAGATGAT GAGGAGATGT CCCTCAGCGC CGAGCCTGAG 
TCCGCGGACA TTCAGGACAG CCCCATGAAC AGCCTGTTGG CCAGCAGCCG TGGGGAGCCT 
CCCGGCGAGG CACCGGAGCT CCCCAGGGGC TCCAAGCCCG AGCCGCCGAG CCAGGGCAGC 
GCCGGGGACG CTGCCGGGAA CGGGAGCCTG GAGCTGGCCG AGCCGGTTGA GGAAGCGGCA 
GGTACCAACG AGGCAGGAGA CGACGGCGCG GGCTCTGTGG GACCTGACGG GGCTGGGGAG 
CTTCTGGGCC CGGGGCGTGA GGACGCCGAG CCCGCCCTGA GTGCGGAAGA ACTGGCGGAG 
CGGCTGGAGC TGGGTGGGGA GGTGCCCCCA CCGGGGCTGC TGGAGGACAG CGAAGGCCCG 
GGCGGCACCT GGGAGCCCGA GGCAGAGGAA GAGGCCACAG AGAGCCGGGC GGGGGGAGAG 
GAGGAGAAGG AGCAGGCGGA GGAGGAGGAG GAGGGCGACG TGTCCGCCCT GGGGCAGGGG 
GAGGACAGGC TGCAGCTGCG GGCCTCGGTG AAGAGGAAAA GCAGGCCGTG CTCCTTGCCG 
GTGTCCGAGC TGGAGACGGT GATCGCGTCC GCCTGCGGAG ACCCCGAGAC CCCGCGGACA 
CACTACATCC GCATCCACAC CCTGCTGCAC AGCATGCCCT CCGCCCAGGG CGGCGGCGCC 
GCGAGCGACG ACGGCGCCGA GGAGGAGGCC ACCCTCCAGG ACGCCTCGGA GAAGGACGGG 
CTCAGCGAGG TGGACACGCT AGCTGCCGCG CCGCCTCCCC TCGAAGAGGA TGGGGAGGAA 
CTCGAGGGGG CTACTCCAGG CACGGCGACC CCCGGCCACT CCGGCCTGAC CAACGGCCTG 
GGCCCGGACG GCGACACGCT CCCCAGCACA GGGAGTGAGA GCGACTCGAG TCCCCGGCAG 
GGTGGGGACC ACAGCTGCGA GGGCTGTGAC ACGTCCTGCT GCAGCCCCTC CTGCTACAGC 
TCCTCGTGCT ACAGCACGTC CTGCTACAGC AGCTCCTGCT ACAGCGCCTC CTGCTATAGC 
CCCTCCTGTT ACAACGGCAG CAGGTTCGCC AGCCACACCC GCTTCTCCTC CGTGGACAGC 
GCCAAGATCT CTGAGAGCAC CGTCTTCTCG TCGCAAGACG ATGAGGAGGA GGAGAACAGT 
GCGTTCGAAT CTGTGCCCGA CTCGGTACAG AGCCCGGAGC TGGACCCGGA GTCGGCAAAT 
GGCGCCGGGC CGTGGCACGA CGAGCTGGCC GCCCCCGCCG CCAGCGTGGC AAGAACCGTG 
GAAGGTCTGG AATCCCCCGT GGCAGGGCCA AGCAATCGGA GAGAAGGTGA ATGTCCTATA 
CTCCATAATT CCCAGCCAGT AAGCCAGCTT CCTTCCTTGA GGCCTGAACA TCATCACTAC 
CCAACAATCG ATGAGCCTCT TCCACCAAAC TGGGAAGCTC GCATTGACAG CCACGGGCGG 
GTCTTTTATG TGGACCACGT GAACCGCACG ACCACCTGGC AGCGGCCGAC AGCAGCAGCC 
ACCCCCGACG GGATGCGGAG ATCGGGGTCC ATCCAGCAGA TGGAGCAACT CAACAGGCGG 
TATCAAAATA TTCAGCGAAC CATTGCAACA GAGAGACCAG AAGAAGATTC TGGAGGCCAA 
AGTTGTGAGC AGGTCCCAAC AGGAGGCAGT GGAGGCGGTG GGAGCGACTC AGAGGCTGAC 
TCTTCTCAAT CAAGCTTAGA TCTGAGGAGA GAAGGGTCAC TTTCTCCAGT GAACTCACAA 
AAAATCACCT TGTTGCTGCA GTCCCCAGCA GTGAAGTTCA TTACCAATCC CGAGTTTTTC 
ACTGTACTAC ACGCCAATTA TGGTGCCTAC CGAGTCTTCA CCAGTAGCAC CTGCTTAAAG 
CACATGATTC TGAAAGTCCG GCGGGACGCT CGCAATTTCG AACGCTACCA GCACAACCGT 
GACTTGGTGA ATTTCATCAA CATGTTTGCA GACACACGGC TGGAATTGCC CCGGGGCTGG 
GAGATCAAAA CGGACCAGCA GGGAAAGTCT TTTTTCGTGG ACCACAATAG TCGAGCCACC 
ACTTTCATTG ACCCCCGAAT CCCTCTTCAG AACGGTCGTC TTCCCAATCA CCTGACACAT 
CGACAGCACC TCCAGAGGCT CCGGAGTTAC AGCGCTGGAG AGGCTTCAGA AGTCTCTCGA 
AACAGAGGAG CCTCTTTACT GGCCAGGCCA GGACACAGCC TGGTAGCTGC TATTCGAAGC 
CAACATCAAC ATGAGTCATT GCCACTAGCA TATAATGACA AGATTGTGGC ATTTCTCCGC 
CAGCCAAACA TTTTTGAAAT GCTGCAAGAG CGTCAGCCAA GCCTGGCGAG AAACCACACA 
CTCAGGGAGA AAATCCATTA CATTCGGACT GAGGGTAATC ATGGGCTTGA AAAGTTGTCC 
TGTGATGCAG ATCTAGTCAT TTTGCTGAGT CTCTTTGAAG AAGAGATTAT GTCCTACGTC 
CCACTGCAGA CTGCCTTTCA TCCCGGGTAC AGCTTCTCTC CCCGCTGTTC ACCCTGTTCC 
TCACCTCAGA ACTCCCCAGG TTTACAGAGA GCCAGTGCAA GAGCCCCTTC ACCCTACCGG 
AGAGACTTCG AGGCCAAGCT CCGCAATTTC TACCGAAAAC TGGAAGCCAA AGGATTTGGT 
CAGGGTCCAG GGAAAATTAA GCTCATCATT CGTCGGGACC ACTTGTTAGA AGGAACCTTC 
AATCAGGTGA TGGCCTATTC CCGGAAGGAG CTTCAGCGAA ACAAACTCTA CGTTACCTTT 
GTTGGAGAGG AGGGTCTGGA CTACAGTGGT CCTTCGCGAG AGTTCTTCTT CCTTTTGTCT 
CAGGAGCTCT TCAACCCTTA CTACGGACTC TTTGAGTACT CTGCAAATGA TACGTACACG 
GTGCAGATCA GCCCCATGTC GGCATTTGTG GAAAACCATC TTGAATGGTT CAGGTTTAGT 
GGTCGGATCC TAGGCCTGGC TTTGATCCAT CAGTACCTTC TTGATGCCTT CTTCACAAGG 
CCCTTCTATA AGGCACTCCT GAGACTGCCC TGTGATTTAA GTGATCTGGA ATATCTGGAT 
GAGGAATTTC ACCAGAGTTT GCAATGGATG AAGGACAACA ACATCACAGA TATCCTAGAC 
CTCACTTTCA CTGTTAACGA AGAAGTTTTT GGACAGGTAA CGGAAAGGGA GTTGAAATCT 
GGAGGAGCCA ACACGCAGGT GACGGAGAAG AACAAGAAGG AGTACATCGA GAGGATGGTG 
AAGTGGCGGG TGGAGCGCGG TGTGGTGCAG CAGACCGAGG CCCTGGTGCG GGGCTTCTAT 
GAGGTGGTAG ACTCGAGGCT TGTCTCTGTG TTCGACGCCA GGGAGCTGGA GCTGGTGATA 
GCAGGCACGG CGGAAATCGA CCTGAATGAC TGGCGGAATA ACACAGAGTA CCGGGGAGGT 
TATCATGACG GGCATCTTGT GATCCGCTGG TTCTGGGCCG CCGTGGAGCG CTTCAATAAT 
GAGCAGAGAC TGAGATTACT TCAGTTCGTC ACGGGAACGT CAAGCGTGCC CTACGAAGGC 
TTCGCAGCCC TTCGAGGAAG CAATGGGCTT CGGCGTTTCT GCATAGAGAA GTGGGGGAAG 
ATTACGTCTC TCCCCAGGGC ACACACATGC TTCAACCGAC TGGATCTTCC ACCTTATCCC 
TCATACTCCA TGTTATATGA AAAGCTGCTA ACAGCAGTAG AAGAAACCAG CACCTTTGGA 
CTTGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_026927394.1
CDS	122..4930
Translation	MLLHLCSVKNLYQNRFLGLAAMASPSRNSQNRRRCKEPLRHSYNPGQFHSMAIRTGPHGTVTIPRSTSDTDLVTSDSRSTLMVSSSYYSIGHSQDLVIHWDIKEEVDAGDWIGMYLIDEVLSENFLDYKNRGVNGSHRGQIIWKIDASSYFVEPETKICFKYYHGVSGALRATTPSVTVKNSAAPIFKSIGSDETVQGQGSRRLISFSLSDFQAMGLKKGMFFNPDPYLKISIQPGKHSIFPALPHHGQERRSKIIGNTVNPIWQAEQFSFVSLPTDVLEIEVKDKFAKSRPIIKRFLGKLSMPVQRLLERHAIGDRVVSYTLGRRLPTDHVSGQLQFRFEITSSIHPDDEEMSLSAEPESADIQDSPMNSLLASSRGEPPGEAPELPRGSKPEPPSQGSAGDAAGNGSLELAEPVEEAAGTNEAGDDGAGSVGPDGAGELLGPGREDAEPALSAEELAERLELGGEVPPPGLLEDSEGPGGTWEPEAEEEATESRAGGEEEKEQAEEEEEGDVSALGQGEDRLQLRASVKRKSRPCSLPVSELETVIASACGDPETPRTHYIRIHTLLHSMPSAQGGGAASDDGAEEEATLQDASEKDGLSEVDTLAAAPPPLEEDGEELEGATPGTATPGHSGLTNGLGPDGDTLPSTGSESDSSPRQGGDHSCEGCDTSCCSPSCYSSSCYSTSCYSSSCYSASCYSPSCYNGSRFASHTRFSSVDSAKISESTVFSSQDDEEEENSAFESVPDSVQSPELDPESANGAGPWHDELAAPAASVARTVEGLESPVAGPSNRREGECPILHNSQPVSQLPSLRPEHHHYPTIDEPLPPNWEARIDSHGRVFYVDHVNRTTTWQRPTAAATPDGMRRSGSIQQMEQLNRRYQNIQRTIATERPEEDSGGQSCEQVPTGGSGGGGSDSEADSSQSSLDLRREGSLSPVNSQKITLLLQSPAVKFITNPEFFTVLHANYGAYRVFTSSTCLKHMILKVRRDARNFERYQHNRDLVNFINMFADTRLELPRGWEIKTDQQGKSFFVDHNSRATTFIDPRIPLQNGRLPNHLTHRQHLQRLRSYSAGEASEVSRNRGASLLARPGHSLVAAIRSQHQHESLPLAYNDKIVAFLRQPNIFEMLQERQPSLARNHTLREKIHYIRTEGNHGLEKLSCDADLVILLSLFEEEIMSYVPLQTAFHPGYSFSPRCSPCSSPQNSPGLQRASARAPSPYRRDFEAKLRNFYRKLEAKGFGQGPGKIKLIIRRDHLLEGTFNQVMAYSRKELQRNKLYVTFVGEEGLDYSGPSREFFFLLSQELFNPYYGLFEYSANDTYTVQISPMSAFVENHLEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRLPCDLSDLEYLDEEFHQSLQWMKDNNITDILDLTFTVNEEVFGQVTERELKSGGANTQVTEKNKKEYIERMVKWRVERGVVQQTEALVRGFYEVVDSRLVSVFDARELELVIAGTAEIDLNDWRNNTEYRGGYHDGHLVIRWFWAAVERFNNEQRLRLLQFVTGTSSVPYEGFAALRGSNGLRRFCIEKWGKITSLPRAHTCFNRLDLPPYPSYSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_027071593.1
Organism	Acinonyx jubatus(cheetah)
Definition	Acinonyx jubatus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_027071593.1

ORF Insert Sequence:

ATGCTGCTGC ACCTCTGTAG TGTGAAGAAT CTGTACCAGA ACAGGTTTTT AGGCCTGGCC 
GCCATGGCAT CCCCATCTAG AAACTCCCAG AATCGCCGCC GGTGCAAGGA GCCACTCCGT 
CACAGCTACA ACCCTGGCCA GTTCCACAGC ATGGCCATCA GGACCGGCCC CCACGGCACC 
GTCACCATAC CCCGCTCCAC CAGCGACACC GACCTGGTCA CCTCAGACAG CCGCTCCACA 
CTTATGGTCA GCAGCTCCTA CTACTCCATA GGACACTCTC AGGACCTGGT GATCCACTGG 
GACATCAAGG AGGAAGTGGA TGCTGGAGAC TGGATTGGCA TGTATCTCAT TGATGAGGTC 
TTGTCAGAAA ACTTTTTGGA CTATAAGAAC CGTGGAGTCA ATGGTTCTCA TCGGGGCCAG 
ATCATCTGGA AGATTGATGC CAGCTCTTAC TTTGTGGAAC CCGAAACTAA GATCTGCTTC 
AAATACTACC ATGGCGTGAG TGGAGCCCTG CGAGCCACCA CCCCCAGTGT CACGGTCAAA 
AACTCTGCAG CTCCTATTTT TAAAAGCATT GGCTCTGATG AGACCGTCCA AGGGCAAGGA 
AGTCGGAGGC TGATCAGCTT TTCTCTCTCA GATTTCCAGG CCATGGGGCT GAAGAAAGGG 
ATGTTTTTCA ACCCAGACCC TTACCTGAAG ATTTCCATTC AGCCTGGAAA GCACAGCATC 
TTCCCTGCCC TCCCACACCA TGGACAGGAG AGGAGATCCA AGATCATAGG CAACACTGTG 
AACCCCATCT GGCAGGCCGA GCAATTCAGC TTTGTGTCCT TGCCCACTGA TGTACTGGAA 
ATTGAGGTGA AAGACAAGTT TGCTAAGAGC CGCCCCATCA TCAAGCGCTT CTTGGGAAAG 
CTGTCGATGC CCGTTCAAAG ACTCCTGGAG AGACACGCCA TAGGGGATAG GGTGGTCAGC 
TATACACTTG GCCGCAGGCT TCCAACAGAT CATGTGAGTG GACAGCTGCA ATTCCGATTT 
GAGATCACTT CTTCCATCCA CCCAGATGAT GAGGAGATGT CCCTCAGCGC CGAGCCTGAG 
TCCGCGGACA TTCAGGACAG CCCCATGAAC AGCCTGTTGG CCAGCAGCCG TGGGGAGCCT 
CCCGGCGAGG CACCGGAGCT CCCCAGGGGC TCCAAGCCCG AGCCGCCGAG CCAGGGCAGC 
GCCGGGGACG CTGCCGGGAA CGGGAGCCTG GAGCTGGCCG AGCCGGTTGA GGAAGCGGCA 
GGTACCAACG AGGCAGGAGA CGACGGCGCG GGCTCTGTGG GACCTGACGG GGCTGGGGAG 
CTTCTGGGCC CGGGGCGTGA GGACGCCGAG CCCGCCCTGA GTGCGGAAGA ACTGGCGGAG 
CGGCTGGAGC TGGGTGGGGA GGTGCCCCCA CCGGGGCTGC TGGAGGACAG CGAAGGCCCG 
GGCGGCACCT GGGAGCCCGA GGCAGAGGAA GAGGCCACAG AGAGCCGGGC GGGGGGAGAG 
GAGGAGAAGG AGCAGGCGGA GGAGGAGGAG GAGGGCGACG TGTCCGCCCT GGGGCAGGGG 
GAGGACAGGC TGCAGCTGCG GGCCTCGGTG AAGAGGAAAA GCAGGCCGTG CTCCTTGCCG 
GTGTCCGAGC TGGAGACGGT GATCGCGTCC GCCTGCGGAG ACCCCGAGAC CCCGCGGACA 
CACTACATCC GCATCCACAC CCTGCTGCAC AGCATGCCCT CCGCCCAGGG CGGCGGCGCC 
GCGAGCGACG ACGGCGCCGA GGAGGAGGCC ACCCTCCAGG ACGCCTCGGA GAAGGACGGG 
CTCAGCGAGG TGGACACGCT AGCTGCCGCG CCGCCTCCCC TCGAAGAGGA TGGGGAGGAA 
CTCGAGGGGG CTACTCCAGG CACGGCGACC CCCGGCCACT CCGGCCTGAC CAACGGCCTG 
GGCCCGGACG GCGACACGCT CCCCAGCACA GGGAGTGAGA GCGACTCGAG TCCCCGGCAG 
GGTGGGGACC ACAGCTGCGA GGGCTGTGAC ACGTCCTGCT GCAGCCCCTC CTGCTACAGC 
TCCTCGTGCT ACAGCACGTC CTGCTACAGC AGCTCCTGCT ACAGCGCCTC CTGCTATAGC 
CCCTCCTGTT ACAACGGCAG CAGGTTCGCC AGCCACACCC GCTTCTCCTC CGTGGACAGC 
GCCAAGATCT CTGAGAGCAC CGTCTTCTCG TCGCAAGACG ATGAGGAGGA GGAGAACAGT 
GCGTTCGAAT CTGTGCCCGA CTCGGTACAG AGCCCGGAGC TGGACCCGGA GTCGGCAAAT 
GGCGCCGGGC CGTGGCACGA CGAGCTGGCC GCCCCCGCCG CCAGCGTGGC AAGAACCGTG 
GAAGGTCTGG AATCCCCCGT GGCAGGGCCA AGCAATCGGA GAGAAGGTGA ATGTCCTATA 
CTCCATAATT CCCAGCCAGT AAGCCAGCTT CCTTCCTTGA GGCCTGAACA TCATCACTAC 
CCAACAATCG ATGAGCCTCT TCCACCAAAC TGGGAAGCTC GCATTGACAG CCACGGGCGG 
GTCTTTTATG TGGACCACGT GAACCGCACG ACCACCTGGC AGCGGCCGAC AGCAGCAGCC 
ACCCCCGACG GGATGCGGAG ATCGGGGTCC ATCCAGCAGA TGGAGCAACT CAACAGGCGG 
TATCAAAATA TTCAGCGAAC CATTGCAACA GAGAGACCAG AAGAAGATTC TGGAGGCCAA 
AGTTGTGAGC AGGTCCCAAC AGGAGGCAGT GGAGGCGGTG GGAGCGACTC AGAGGCTGAC 
TCTTCTCAAT CAAGCTTAGA TCTGAGGAGA GAAGGGTCAC TTTCTCCAGT GAACTCACAA 
AAAATCACCT TGTTGCTGCA GTCCCCAGCA GTGAAGTTCA TTACCAATCC CGAGTTTTTC 
ACTGTACTAC ACGCCAATTA TGGTGCCTAC CGAGTCTTCA CCAGTAGCAC CTGCTTAAAG 
CACATGATTC TGAAAGTCCG GCGGGACGCT CGCAATTTCG AACGCTACCA GCACAACCGT 
GACTTGGTGA ATTTCATCAA CATGTTTGCA GACACACGGC TGGAATTGCC CCGGGGCTGG 
GAGATCAAAA CGGACCAGCA GGGAAAGTCT TTTTTCGTGG ACCACAATAG TCGAGCCACC 
ACTTTCATTG ACCCCCGAAT CCCTCTTCAG AACGGTCGTC TTCCCAATCA CCTGACACAT 
CGACAGCACC TCCAGAGGCT CCGGAGTTAC AGCGCTGGAG AGGCTTCAGA AGTCTCTCGA 
AACAGAGGAG CCTCTTTACT GGCCAGGCCA GGACACAGCC TGGTAGCTGC TATTCGAAGC 
CAACATCAAC ATGAGTCATT GCCACTAGCA TATAATGACA AGATTGTGGC ATTTCTCCGC 
CAGCCAAACA TTTTTGAAAT GCTGCAAGAG CGTCAGCCAA GCCTGGCGAG AAACCACACA 
CTCAGGGAGA AAATCCATTA CATTCGGACT GAGGGTAATC ATGGGCTTGA AAAGTTGTCC 
TGTGATGCAG ATCTAGTCAT TTTGCTGAGT CTCTTTGAAG AAGAGATTAT GTCCTACGTC 
CCACTGCAGA CTGCCTTTCA TCCCGGGTAC AGCTTCTCTC CCCGCTGTTC ACCCTGTTCC 
TCACCTCAGA ACTCCCCAGG TTTACAGAGA GCCAGTGCAA GAGCCCCTTC ACCCTACCGG 
AGAGACTTCG AGGCCAAGCT CCGCAATTTC TACCGAAAAC TGGAAGCCAA AGGATTTGGT 
CAGGGTCCAG GGAAAATTAA GCTCATCATT CGTCGGGACC ACTTGTTAGA AGGAACCTTC 
AATCAGGTGA TGGCCTATTC CCGGAAGGAG CTTCAGCGAA ACAAACTCTA CGTTACCTTT 
GTTGGAGAGG AGGGTCTGGA CTACAGTGGT CCTTCGCGAG AGTTCTTCTT CCTTTTGTCT 
CAGGAGCTCT TCAACCCTTA CTACGGACTC TTTGAGTACT CTGCAAATGA TACGTACACG 
GTGCAGATCA GCCCCATGTC GGCATTTGTG GAAAACCATC TTGAATGGTT CAGGTTTAGT 
GGTCGGATCC TAGGCCTGGC TTTGATCCAT CAGTACCTTC TTGATGCCTT CTTCACAAGG 
CCCTTCTATA AGGCACTCCT GAGACTGCCC TGTGATTTAA GTGATCTGGA ATATCTGGAT 
GAGGAATTTC ACCAGAGTTT GCAATGGATG AAGGACAACA ACATCACAGA TATCCTAGAC 
CTCACTTTCA CTGTTAACGA AGAAGTTTTT GGACAGGTAA CGGAAAGGGA GTTGAAATCT 
GGAGGAGCCA ACACGCAGGT GACGGAGAAG AACAAGAAGG AGTACATCGA GAGGATGGTG 
AAGTGGCGGG TGGAGCGCGG TGTGGTGCAG CAGACCGAGG CCCTGGTGCG GGGCTTCTAT 
GAGGTGGTAG ACTCGAGGCT TGTCTCTGTG TTCGACGCCA GGGAGCTGGA GCTGGTGATA 
GCAGGCACGG CGGAAATCGA CCTGAATGAC TGGCGGAATA ACACAGAGTA CCGGGGAGGT 
TATCATGACG GGCATCTTGT GATCCGCTGG TTCTGGGCCG CCGTGGAGCG CTTCAATAAT 
GAGCAGAGAC TGAGATTACT TCAGTTCGTC ACGGGAACGT CAAGCGTGCC CTACGAAGGC 
TTCGCAGCCC TTCGAGGAAG CAATGGGCTT CGGCGTTTCT GCATAGAGAA GTGGGGGAAG 
ATTACGTCTC TCCCCAGGGC ACACACATGC TTCAACCGAC TGGATCTTCC ACCTTATCCC 
TCATACTCCA TGTTATATGA AAAGCTGCTA ACAGCAGTAG AAGAAACCAG CACCTTTGGA 
CTTGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HECW1 ( XM_027071594.1 ) cDNA ORF clone, Acinonyx jubatus(cheetah) -> XP_026927395.1 Acinonyx jubatus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X3, mRNA.

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CloneID	OAc228959
Clone ID Related Accession (Same CDS sequence)	XM_027071594.1
Accession Version	XM_027071594.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4803bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-12
Organism	Acinonyx jubatus(cheetah)
Product	E3 ubiquitin-protein ligase HECW1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_020834755.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Acinonyx jubatus Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCTACC TGAAAAGGCC AGACTTTAAA GACCCAGGCA GTGTCGTGGT CCAAGGCGTC 
TCAAAGCAGG TGGCCAGATC TGCGTTTCTC ATCAGCGGAC TCGCTCCGGC TGTGGCTATT 
ACGAATCTGT ACCAGAACAG GTTTTTAGGC CTGGCCGCCA TGGCATCCCC ATCTAGAAAC 
TCCCAGAATC GCCGCCGGTG CAAGGAGCCA CTCCGTCACA GCTACAACCC TGGCCAGTTC 
CACAGCATGG CCATCAGGAC CGGCCCCCAC GGCACCGTCA CCATACCCCG CTCCACCAGC 
GACACCGACC TGGTCACCTC AGACAGCCGC TCCACACTTA TGGTCAGCAG CTCCTACTAC 
TCCATAGGAC ACTCTCAGGA CCTGGTGATC CACTGGGACA TCAAGGAGGA AGTGGATGCT 
GGAGACTGGA TTGGCATGTA TCTCATTGAT GAGGTCTTGT CAGAAAACTT TTTGGACTAT 
AAGAACCGTG GAGTCAATGG TTCTCATCGG GGCCAGATCA TCTGGAAGAT TGATGCCAGC 
TCTTACTTTG TGGAACCCGA AACTAAGATC TGCTTCAAAT ACTACCATGG CGTGAGTGGA 
GCCCTGCGAG CCACCACCCC CAGTGTCACG GTCAAAAACT CTGCAGCTCC TATTTTTAAA 
AGCATTGGCT CTGATGAGAC CGTCCAAGGG CAAGGAAGTC GGAGGCTGAT CAGCTTTTCT 
CTCTCAGATT TCCAGGCCAT GGGGCTGAAG AAAGGGATGT TTTTCAACCC AGACCCTTAC 
CTGAAGATTT CCATTCAGCC TGGAAAGCAC AGCATCTTCC CTGCCCTCCC ACACCATGGA 
CAGGAGAGGA GATCCAAGAT CATAGGCAAC ACTGTGAACC CCATCTGGCA GGCCGAGCAA 
TTCAGCTTTG TGTCCTTGCC CACTGATGTA CTGGAAATTG AGGTGAAAGA CAAGTTTGCT 
AAGAGCCGCC CCATCATCAA GCGCTTCTTG GGAAAGCTGT CGATGCCCGT TCAAAGACTC 
CTGGAGAGAC ACGCCATAGG GGATAGGGTG GTCAGCTATA CACTTGGCCG CAGGCTTCCA 
ACAGATCATG TGAGTGGACA GCTGCAATTC CGATTTGAGA TCACTTCTTC CATCCACCCA 
GATGATGAGG AGATGTCCCT CAGCGCCGAG CCTGAGTCCG CGGACATTCA GGACAGCCCC 
ATGAACAGCC TGTTGGCCAG CAGCCGTGGG GAGCCTCCCG GCGAGGCACC GGAGCTCCCC 
AGGGGCTCCA AGCCCGAGCC GCCGAGCCAG GGCAGCGCCG GGGACGCTGC CGGGAACGGG 
AGCCTGGAGC TGGCCGAGCC GGTTGAGGAA GCGGCAGGTA CCAACGAGGC AGGAGACGAC 
GGCGCGGGCT CTGTGGGACC TGACGGGGCT GGGGAGCTTC TGGGCCCGGG GCGTGAGGAC 
GCCGAGCCCG CCCTGAGTGC GGAAGAACTG GCGGAGCGGC TGGAGCTGGG TGGGGAGGTG 
CCCCCACCGG GGCTGCTGGA GGACAGCGAA GGCCCGGGCG GCACCTGGGA GCCCGAGGCA 
GAGGAAGAGG CCACAGAGAG CCGGGCGGGG GGAGAGGAGG AGAAGGAGCA GGCGGAGGAG 
GAGGAGGAGG GCGACGTGTC CGCCCTGGGG CAGGGGGAGG ACAGGCTGCA GCTGCGGGCC 
TCGGTGAAGA GGAAAAGCAG GCCGTGCTCC TTGCCGGTGT CCGAGCTGGA GACGGTGATC 
GCGTCCGCCT GCGGAGACCC CGAGACCCCG CGGACACACT ACATCCGCAT CCACACCCTG 
CTGCACAGCA TGCCCTCCGC CCAGGGCGGC GGCGCCGCGA GCGACGACGG CGCCGAGGAG 
GAGGCCACCC TCCAGGACGC CTCGGAGAAG GACGGGCTCA GCGAGGTGGA CACGCTAGCT 
GCCGCGCCGC CTCCCCTCGA AGAGGATGGG GAGGAACTCG AGGGGGCTAC TCCAGGCACG 
GCGACCCCCG GCCACTCCGG CCTGACCAAC GGCCTGGGCC CGGACGGCGA CACGCTCCCC 
AGCACAGGGA GTGAGAGCGA CTCGAGTCCC CGGCAGGGTG GGGACCACAG CTGCGAGGGC 
TGTGACACGT CCTGCTGCAG CCCCTCCTGC TACAGCTCCT CGTGCTACAG CACGTCCTGC 
TACAGCAGCT CCTGCTACAG CGCCTCCTGC TATAGCCCCT CCTGTTACAA CGGCAGCAGG 
TTCGCCAGCC ACACCCGCTT CTCCTCCGTG GACAGCGCCA AGATCTCTGA GAGCACCGTC 
TTCTCGTCGC AAGACGATGA GGAGGAGGAG AACAGTGCGT TCGAATCTGT GCCCGACTCG 
GTACAGAGCC CGGAGCTGGA CCCGGAGTCG GCAAATGGCG CCGGGCCGTG GCACGACGAG 
CTGGCCGCCC CCGCCGCCAG CGTGGCAAGA ACCGTGGAAG GTCTGGAATC CCCCGTGGCA 
GGGCCAAGCA ATCGGAGAGA AGACTGGGAA GCTCGCATTG ACAGCCACGG GCGGGTCTTT 
TATGTGGACC ACGTGAACCG CACGACCACC TGGCAGCGGC CGACAGCAGC AGCCACCCCC 
GACGGGATGC GGAGATCGGG GTCCATCCAG CAGATGGAGC AACTCAACAG GCGGTATCAA 
AATATTCAGC GAACCATTGC AACAGAGAGA CCAGAAGAAG ATTCTGGAGG CCAAAGTTGT 
GAGCAGGTCC CAACAGGAGG CAGTGGAGGC GGTGGGAGCG ACTCAGAGGC TGACTCTTCT 
CAATCAAGCT TAGATCTGAG GAGAGAAGGG TCACTTTCTC CAGTGAACTC ACAAAAAATC 
ACCTTGTTGC TGCAGTCCCC AGCAGTGAAG TTCATTACCA ATCCCGAGTT TTTCACTGTA 
CTACACGCCA ATTATGGTGC CTACCGAGTC TTCACCAGTA GCACCTGCTT AAAGCACATG 
ATTCTGAAAG TCCGGCGGGA CGCTCGCAAT TTCGAACGCT ACCAGCACAA CCGTGACTTG 
GTGAATTTCA TCAACATGTT TGCAGACACA CGGCTGGAAT TGCCCCGGGG CTGGGAGATC 
AAAACGGACC AGCAGGGAAA GTCTTTTTTC GTGGACCACA ATAGTCGAGC CACCACTTTC 
ATTGACCCCC GAATCCCTCT TCAGAACGGT CGTCTTCCCA ATCACCTGAC ACATCGACAG 
CACCTCCAGA GGCTCCGGAG TTACAGCGCT GGAGAGGCTT CAGAAGTCTC TCGAAACAGA 
GGAGCCTCTT TACTGGCCAG GCCAGGACAC AGCCTGGTAG CTGCTATTCG AAGCCAACAT 
CAACATGAGT CATTGCCACT AGCATATAAT GACAAGATTG TGGCATTTCT CCGCCAGCCA 
AACATTTTTG AAATGCTGCA AGAGCGTCAG CCAAGCCTGG CGAGAAACCA CACACTCAGG 
GAGAAAATCC ATTACATTCG GACTGAGGGT AATCATGGGC TTGAAAAGTT GTCCTGTGAT 
GCAGATCTAG TCATTTTGCT GAGTCTCTTT GAAGAAGAGA TTATGTCCTA CGTCCCACTG 
CAGACTGCCT TTCATCCCGG GTACAGCTTC TCTCCCCGCT GTTCACCCTG TTCCTCACCT 
CAGAACTCCC CAGGTTTACA GAGAGCCAGT GCAAGAGCCC CTTCACCCTA CCGGAGAGAC 
TTCGAGGCCA AGCTCCGCAA TTTCTACCGA AAACTGGAAG CCAAAGGATT TGGTCAGGGT 
CCAGGGAAAA TTAAGCTCAT CATTCGTCGG GACCACTTGT TAGAAGGAAC CTTCAATCAG 
GTGATGGCCT ATTCCCGGAA GGAGCTTCAG CGAAACAAAC TCTACGTTAC CTTTGTTGGA 
GAGGAGGGTC TGGACTACAG TGGTCCTTCG CGAGAGTTCT TCTTCCTTTT GTCTCAGGAG 
CTCTTCAACC CTTACTACGG ACTCTTTGAG TACTCTGCAA ATGATACGTA CACGGTGCAG 
ATCAGCCCCA TGTCGGCATT TGTGGAAAAC CATCTTGAAT GGTTCAGGTT TAGTGGTCGG 
ATCCTAGGCC TGGCTTTGAT CCATCAGTAC CTTCTTGATG CCTTCTTCAC AAGGCCCTTC 
TATAAGGCAC TCCTGAGACT GCCCTGTGAT TTAAGTGATC TGGAATATCT GGATGAGGAA 
TTTCACCAGA GTTTGCAATG GATGAAGGAC AACAACATCA CAGATATCCT AGACCTCACT 
TTCACTGTTA ACGAAGAAGT TTTTGGACAG GTAACGGAAA GGGAGTTGAA ATCTGGAGGA 
GCCAACACGC AGGTGACGGA GAAGAACAAG AAGGAGTACA TCGAGAGGAT GGTGAAGTGG 
CGGGTGGAGC GCGGTGTGGT GCAGCAGACC GAGGCCCTGG TGCGGGGCTT CTATGAGGTG 
GTAGACTCGA GGCTTGTCTC TGTGTTCGAC GCCAGGGAGC TGGAGCTGGT GATAGCAGGC 
ACGGCGGAAA TCGACCTGAA TGACTGGCGG AATAACACAG AGTACCGGGG AGGTTATCAT 
GACGGGCATC TTGTGATCCG CTGGTTCTGG GCCGCCGTGG AGCGCTTCAA TAATGAGCAG 
AGACTGAGAT TACTTCAGTT CGTCACGGGA ACGTCAAGCG TGCCCTACGA AGGCTTCGCA 
GCCCTTCGAG GAAGCAATGG GCTTCGGCGT TTCTGCATAG AGAAGTGGGG GAAGATTACG 
TCTCTCCCCA GGGCACACAC ATGCTTCAAC CGACTGGATC TTCCACCTTA TCCCTCATAC 
TCCATGTTAT ATGAAAAGCT GCTAACAGCA GTAGAAGAAA CCAGCACCTT TGGACTTGAA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_026927395.1
CDS	50..4852
Translation	MPYLKRPDFKDPGSVVVQGVSKQVARSAFLISGLAPAVAITNLYQNRFLGLAAMASPSRNSQNRRRCKEPLRHSYNPGQFHSMAIRTGPHGTVTIPRSTSDTDLVTSDSRSTLMVSSSYYSIGHSQDLVIHWDIKEEVDAGDWIGMYLIDEVLSENFLDYKNRGVNGSHRGQIIWKIDASSYFVEPETKICFKYYHGVSGALRATTPSVTVKNSAAPIFKSIGSDETVQGQGSRRLISFSLSDFQAMGLKKGMFFNPDPYLKISIQPGKHSIFPALPHHGQERRSKIIGNTVNPIWQAEQFSFVSLPTDVLEIEVKDKFAKSRPIIKRFLGKLSMPVQRLLERHAIGDRVVSYTLGRRLPTDHVSGQLQFRFEITSSIHPDDEEMSLSAEPESADIQDSPMNSLLASSRGEPPGEAPELPRGSKPEPPSQGSAGDAAGNGSLELAEPVEEAAGTNEAGDDGAGSVGPDGAGELLGPGREDAEPALSAEELAERLELGGEVPPPGLLEDSEGPGGTWEPEAEEEATESRAGGEEEKEQAEEEEEGDVSALGQGEDRLQLRASVKRKSRPCSLPVSELETVIASACGDPETPRTHYIRIHTLLHSMPSAQGGGAASDDGAEEEATLQDASEKDGLSEVDTLAAAPPPLEEDGEELEGATPGTATPGHSGLTNGLGPDGDTLPSTGSESDSSPRQGGDHSCEGCDTSCCSPSCYSSSCYSTSCYSSSCYSASCYSPSCYNGSRFASHTRFSSVDSAKISESTVFSSQDDEEEENSAFESVPDSVQSPELDPESANGAGPWHDELAAPAASVARTVEGLESPVAGPSNRREDWEARIDSHGRVFYVDHVNRTTTWQRPTAAATPDGMRRSGSIQQMEQLNRRYQNIQRTIATERPEEDSGGQSCEQVPTGGSGGGGSDSEADSSQSSLDLRREGSLSPVNSQKITLLLQSPAVKFITNPEFFTVLHANYGAYRVFTSSTCLKHMILKVRRDARNFERYQHNRDLVNFINMFADTRLELPRGWEIKTDQQGKSFFVDHNSRATTFIDPRIPLQNGRLPNHLTHRQHLQRLRSYSAGEASEVSRNRGASLLARPGHSLVAAIRSQHQHESLPLAYNDKIVAFLRQPNIFEMLQERQPSLARNHTLREKIHYIRTEGNHGLEKLSCDADLVILLSLFEEEIMSYVPLQTAFHPGYSFSPRCSPCSSPQNSPGLQRASARAPSPYRRDFEAKLRNFYRKLEAKGFGQGPGKIKLIIRRDHLLEGTFNQVMAYSRKELQRNKLYVTFVGEEGLDYSGPSREFFFLLSQELFNPYYGLFEYSANDTYTVQISPMSAFVENHLEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRLPCDLSDLEYLDEEFHQSLQWMKDNNITDILDLTFTVNEEVFGQVTERELKSGGANTQVTEKNKKEYIERMVKWRVERGVVQQTEALVRGFYEVVDSRLVSVFDARELELVIAGTAEIDLNDWRNNTEYRGGYHDGHLVIRWFWAAVERFNNEQRLRLLQFVTGTSSVPYEGFAALRGSNGLRRFCIEKWGKITSLPRAHTCFNRLDLPPYPSYSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_027071594.1
Organism	Acinonyx jubatus(cheetah)
Definition	Acinonyx jubatus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_027071594.1

ORF Insert Sequence:

ATGCCCTACC TGAAAAGGCC AGACTTTAAA GACCCAGGCA GTGTCGTGGT CCAAGGCGTC 
TCAAAGCAGG TGGCCAGATC TGCGTTTCTC ATCAGCGGAC TCGCTCCGGC TGTGGCTATT 
ACGAATCTGT ACCAGAACAG GTTTTTAGGC CTGGCCGCCA TGGCATCCCC ATCTAGAAAC 
TCCCAGAATC GCCGCCGGTG CAAGGAGCCA CTCCGTCACA GCTACAACCC TGGCCAGTTC 
CACAGCATGG CCATCAGGAC CGGCCCCCAC GGCACCGTCA CCATACCCCG CTCCACCAGC 
GACACCGACC TGGTCACCTC AGACAGCCGC TCCACACTTA TGGTCAGCAG CTCCTACTAC 
TCCATAGGAC ACTCTCAGGA CCTGGTGATC CACTGGGACA TCAAGGAGGA AGTGGATGCT 
GGAGACTGGA TTGGCATGTA TCTCATTGAT GAGGTCTTGT CAGAAAACTT TTTGGACTAT 
AAGAACCGTG GAGTCAATGG TTCTCATCGG GGCCAGATCA TCTGGAAGAT TGATGCCAGC 
TCTTACTTTG TGGAACCCGA AACTAAGATC TGCTTCAAAT ACTACCATGG CGTGAGTGGA 
GCCCTGCGAG CCACCACCCC CAGTGTCACG GTCAAAAACT CTGCAGCTCC TATTTTTAAA 
AGCATTGGCT CTGATGAGAC CGTCCAAGGG CAAGGAAGTC GGAGGCTGAT CAGCTTTTCT 
CTCTCAGATT TCCAGGCCAT GGGGCTGAAG AAAGGGATGT TTTTCAACCC AGACCCTTAC 
CTGAAGATTT CCATTCAGCC TGGAAAGCAC AGCATCTTCC CTGCCCTCCC ACACCATGGA 
CAGGAGAGGA GATCCAAGAT CATAGGCAAC ACTGTGAACC CCATCTGGCA GGCCGAGCAA 
TTCAGCTTTG TGTCCTTGCC CACTGATGTA CTGGAAATTG AGGTGAAAGA CAAGTTTGCT 
AAGAGCCGCC CCATCATCAA GCGCTTCTTG GGAAAGCTGT CGATGCCCGT TCAAAGACTC 
CTGGAGAGAC ACGCCATAGG GGATAGGGTG GTCAGCTATA CACTTGGCCG CAGGCTTCCA 
ACAGATCATG TGAGTGGACA GCTGCAATTC CGATTTGAGA TCACTTCTTC CATCCACCCA 
GATGATGAGG AGATGTCCCT CAGCGCCGAG CCTGAGTCCG CGGACATTCA GGACAGCCCC 
ATGAACAGCC TGTTGGCCAG CAGCCGTGGG GAGCCTCCCG GCGAGGCACC GGAGCTCCCC 
AGGGGCTCCA AGCCCGAGCC GCCGAGCCAG GGCAGCGCCG GGGACGCTGC CGGGAACGGG 
AGCCTGGAGC TGGCCGAGCC GGTTGAGGAA GCGGCAGGTA CCAACGAGGC AGGAGACGAC 
GGCGCGGGCT CTGTGGGACC TGACGGGGCT GGGGAGCTTC TGGGCCCGGG GCGTGAGGAC 
GCCGAGCCCG CCCTGAGTGC GGAAGAACTG GCGGAGCGGC TGGAGCTGGG TGGGGAGGTG 
CCCCCACCGG GGCTGCTGGA GGACAGCGAA GGCCCGGGCG GCACCTGGGA GCCCGAGGCA 
GAGGAAGAGG CCACAGAGAG CCGGGCGGGG GGAGAGGAGG AGAAGGAGCA GGCGGAGGAG 
GAGGAGGAGG GCGACGTGTC CGCCCTGGGG CAGGGGGAGG ACAGGCTGCA GCTGCGGGCC 
TCGGTGAAGA GGAAAAGCAG GCCGTGCTCC TTGCCGGTGT CCGAGCTGGA GACGGTGATC 
GCGTCCGCCT GCGGAGACCC CGAGACCCCG CGGACACACT ACATCCGCAT CCACACCCTG 
CTGCACAGCA TGCCCTCCGC CCAGGGCGGC GGCGCCGCGA GCGACGACGG CGCCGAGGAG 
GAGGCCACCC TCCAGGACGC CTCGGAGAAG GACGGGCTCA GCGAGGTGGA CACGCTAGCT 
GCCGCGCCGC CTCCCCTCGA AGAGGATGGG GAGGAACTCG AGGGGGCTAC TCCAGGCACG 
GCGACCCCCG GCCACTCCGG CCTGACCAAC GGCCTGGGCC CGGACGGCGA CACGCTCCCC 
AGCACAGGGA GTGAGAGCGA CTCGAGTCCC CGGCAGGGTG GGGACCACAG CTGCGAGGGC 
TGTGACACGT CCTGCTGCAG CCCCTCCTGC TACAGCTCCT CGTGCTACAG CACGTCCTGC 
TACAGCAGCT CCTGCTACAG CGCCTCCTGC TATAGCCCCT CCTGTTACAA CGGCAGCAGG 
TTCGCCAGCC ACACCCGCTT CTCCTCCGTG GACAGCGCCA AGATCTCTGA GAGCACCGTC 
TTCTCGTCGC AAGACGATGA GGAGGAGGAG AACAGTGCGT TCGAATCTGT GCCCGACTCG 
GTACAGAGCC CGGAGCTGGA CCCGGAGTCG GCAAATGGCG CCGGGCCGTG GCACGACGAG 
CTGGCCGCCC CCGCCGCCAG CGTGGCAAGA ACCGTGGAAG GTCTGGAATC CCCCGTGGCA 
GGGCCAAGCA ATCGGAGAGA AGACTGGGAA GCTCGCATTG ACAGCCACGG GCGGGTCTTT 
TATGTGGACC ACGTGAACCG CACGACCACC TGGCAGCGGC CGACAGCAGC AGCCACCCCC 
GACGGGATGC GGAGATCGGG GTCCATCCAG CAGATGGAGC AACTCAACAG GCGGTATCAA 
AATATTCAGC GAACCATTGC AACAGAGAGA CCAGAAGAAG ATTCTGGAGG CCAAAGTTGT 
GAGCAGGTCC CAACAGGAGG CAGTGGAGGC GGTGGGAGCG ACTCAGAGGC TGACTCTTCT 
CAATCAAGCT TAGATCTGAG GAGAGAAGGG TCACTTTCTC CAGTGAACTC ACAAAAAATC 
ACCTTGTTGC TGCAGTCCCC AGCAGTGAAG TTCATTACCA ATCCCGAGTT TTTCACTGTA 
CTACACGCCA ATTATGGTGC CTACCGAGTC TTCACCAGTA GCACCTGCTT AAAGCACATG 
ATTCTGAAAG TCCGGCGGGA CGCTCGCAAT TTCGAACGCT ACCAGCACAA CCGTGACTTG 
GTGAATTTCA TCAACATGTT TGCAGACACA CGGCTGGAAT TGCCCCGGGG CTGGGAGATC 
AAAACGGACC AGCAGGGAAA GTCTTTTTTC GTGGACCACA ATAGTCGAGC CACCACTTTC 
ATTGACCCCC GAATCCCTCT TCAGAACGGT CGTCTTCCCA ATCACCTGAC ACATCGACAG 
CACCTCCAGA GGCTCCGGAG TTACAGCGCT GGAGAGGCTT CAGAAGTCTC TCGAAACAGA 
GGAGCCTCTT TACTGGCCAG GCCAGGACAC AGCCTGGTAG CTGCTATTCG AAGCCAACAT 
CAACATGAGT CATTGCCACT AGCATATAAT GACAAGATTG TGGCATTTCT CCGCCAGCCA 
AACATTTTTG AAATGCTGCA AGAGCGTCAG CCAAGCCTGG CGAGAAACCA CACACTCAGG 
GAGAAAATCC ATTACATTCG GACTGAGGGT AATCATGGGC TTGAAAAGTT GTCCTGTGAT 
GCAGATCTAG TCATTTTGCT GAGTCTCTTT GAAGAAGAGA TTATGTCCTA CGTCCCACTG 
CAGACTGCCT TTCATCCCGG GTACAGCTTC TCTCCCCGCT GTTCACCCTG TTCCTCACCT 
CAGAACTCCC CAGGTTTACA GAGAGCCAGT GCAAGAGCCC CTTCACCCTA CCGGAGAGAC 
TTCGAGGCCA AGCTCCGCAA TTTCTACCGA AAACTGGAAG CCAAAGGATT TGGTCAGGGT 
CCAGGGAAAA TTAAGCTCAT CATTCGTCGG GACCACTTGT TAGAAGGAAC CTTCAATCAG 
GTGATGGCCT ATTCCCGGAA GGAGCTTCAG CGAAACAAAC TCTACGTTAC CTTTGTTGGA 
GAGGAGGGTC TGGACTACAG TGGTCCTTCG CGAGAGTTCT TCTTCCTTTT GTCTCAGGAG 
CTCTTCAACC CTTACTACGG ACTCTTTGAG TACTCTGCAA ATGATACGTA CACGGTGCAG 
ATCAGCCCCA TGTCGGCATT TGTGGAAAAC CATCTTGAAT GGTTCAGGTT TAGTGGTCGG 
ATCCTAGGCC TGGCTTTGAT CCATCAGTAC CTTCTTGATG CCTTCTTCAC AAGGCCCTTC 
TATAAGGCAC TCCTGAGACT GCCCTGTGAT TTAAGTGATC TGGAATATCT GGATGAGGAA 
TTTCACCAGA GTTTGCAATG GATGAAGGAC AACAACATCA CAGATATCCT AGACCTCACT 
TTCACTGTTA ACGAAGAAGT TTTTGGACAG GTAACGGAAA GGGAGTTGAA ATCTGGAGGA 
GCCAACACGC AGGTGACGGA GAAGAACAAG AAGGAGTACA TCGAGAGGAT GGTGAAGTGG 
CGGGTGGAGC GCGGTGTGGT GCAGCAGACC GAGGCCCTGG TGCGGGGCTT CTATGAGGTG 
GTAGACTCGA GGCTTGTCTC TGTGTTCGAC GCCAGGGAGC TGGAGCTGGT GATAGCAGGC 
ACGGCGGAAA TCGACCTGAA TGACTGGCGG AATAACACAG AGTACCGGGG AGGTTATCAT 
GACGGGCATC TTGTGATCCG CTGGTTCTGG GCCGCCGTGG AGCGCTTCAA TAATGAGCAG 
AGACTGAGAT TACTTCAGTT CGTCACGGGA ACGTCAAGCG TGCCCTACGA AGGCTTCGCA 
GCCCTTCGAG GAAGCAATGG GCTTCGGCGT TTCTGCATAG AGAAGTGGGG GAAGATTACG 
TCTCTCCCCA GGGCACACAC ATGCTTCAAC CGACTGGATC TTCCACCTTA TCCCTCATAC 
TCCATGTTAT ATGAAAAGCT GCTAACAGCA GTAGAAGAAA CCAGCACCTT TGGACTTGAA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HECW1 ( XM_027071595.1 ) cDNA ORF clone, Acinonyx jubatus(cheetah) -> XP_026927396.1 Acinonyx jubatus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X4, mRNA.

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CloneID	OAc228960
Clone ID Related Accession (Same CDS sequence)	XM_027071595.1 , XM_027071596.1
Accession Version	XM_027071595.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4746bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-12
Organism	Acinonyx jubatus(cheetah)
Product	E3 ubiquitin-protein ligase HECW1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_020834755.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Acinonyx jubatus Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCATCCC CATCTAGAAA CTCCCAGAAT CGCCGCCGGT GCAAGGAGCC ACTCCGTCAC 
AGCTACAACC CTGGCCAGTT CCACAGCATG GCCATCAGGA CCGGCCCCCA CGGCACCGTC 
ACCATACCCC GCTCCACCAG CGACACCGAC CTGGTCACCT CAGACAGCCG CTCCACACTT 
ATGGTCAGCA GCTCCTACTA CTCCATAGGA CACTCTCAGG ACCTGGTGAT CCACTGGGAC 
ATCAAGGAGG AAGTGGATGC TGGAGACTGG ATTGGCATGT ATCTCATTGA TGAGGTCTTG 
TCAGAAAACT TTTTGGACTA TAAGAACCGT GGAGTCAATG GTTCTCATCG GGGCCAGATC 
ATCTGGAAGA TTGATGCCAG CTCTTACTTT GTGGAACCCG AAACTAAGAT CTGCTTCAAA 
TACTACCATG GCGTGAGTGG AGCCCTGCGA GCCACCACCC CCAGTGTCAC GGTCAAAAAC 
TCTGCAGCTC CTATTTTTAA AAGCATTGGC TCTGATGAGA CCGTCCAAGG GCAAGGAAGT 
CGGAGGCTGA TCAGCTTTTC TCTCTCAGAT TTCCAGGCCA TGGGGCTGAA GAAAGGGATG 
TTTTTCAACC CAGACCCTTA CCTGAAGATT TCCATTCAGC CTGGAAAGCA CAGCATCTTC 
CCTGCCCTCC CACACCATGG ACAGGAGAGG AGATCCAAGA TCATAGGCAA CACTGTGAAC 
CCCATCTGGC AGGCCGAGCA ATTCAGCTTT GTGTCCTTGC CCACTGATGT ACTGGAAATT 
GAGGTGAAAG ACAAGTTTGC TAAGAGCCGC CCCATCATCA AGCGCTTCTT GGGAAAGCTG 
TCGATGCCCG TTCAAAGACT CCTGGAGAGA CACGCCATAG GGGATAGGGT GGTCAGCTAT 
ACACTTGGCC GCAGGCTTCC AACAGATCAT GTGAGTGGAC AGCTGCAATT CCGATTTGAG 
ATCACTTCTT CCATCCACCC AGATGATGAG GAGATGTCCC TCAGCGCCGA GCCTGAGTCC 
GCGGACATTC AGGACAGCCC CATGAACAGC CTGTTGGCCA GCAGCCGTGG GGAGCCTCCC 
GGCGAGGCAC CGGAGCTCCC CAGGGGCTCC AAGCCCGAGC CGCCGAGCCA GGGCAGCGCC 
GGGGACGCTG CCGGGAACGG GAGCCTGGAG CTGGCCGAGC CGGTTGAGGA AGCGGCAGGT 
ACCAACGAGG CAGGAGACGA CGGCGCGGGC TCTGTGGGAC CTGACGGGGC TGGGGAGCTT 
CTGGGCCCGG GGCGTGAGGA CGCCGAGCCC GCCCTGAGTG CGGAAGAACT GGCGGAGCGG 
CTGGAGCTGG GTGGGGAGGT GCCCCCACCG GGGCTGCTGG AGGACAGCGA AGGCCCGGGC 
GGCACCTGGG AGCCCGAGGC AGAGGAAGAG GCCACAGAGA GCCGGGCGGG GGGAGAGGAG 
GAGAAGGAGC AGGCGGAGGA GGAGGAGGAG GGCGACGTGT CCGCCCTGGG GCAGGGGGAG 
GACAGGCTGC AGCTGCGGGC CTCGGTGAAG AGGAAAAGCA GGCCGTGCTC CTTGCCGGTG 
TCCGAGCTGG AGACGGTGAT CGCGTCCGCC TGCGGAGACC CCGAGACCCC GCGGACACAC 
TACATCCGCA TCCACACCCT GCTGCACAGC ATGCCCTCCG CCCAGGGCGG CGGCGCCGCG 
AGCGACGACG GCGCCGAGGA GGAGGCCACC CTCCAGGACG CCTCGGAGAA GGACGGGCTC 
AGCGAGGTGG ACACGCTAGC TGCCGCGCCG CCTCCCCTCG AAGAGGATGG GGAGGAACTC 
GAGGGGGCTA CTCCAGGCAC GGCGACCCCC GGCCACTCCG GCCTGACCAA CGGCCTGGGC 
CCGGACGGCG ACACGCTCCC CAGCACAGGG AGTGAGAGCG ACTCGAGTCC CCGGCAGGGT 
GGGGACCACA GCTGCGAGGG CTGTGACACG TCCTGCTGCA GCCCCTCCTG CTACAGCTCC 
TCGTGCTACA GCACGTCCTG CTACAGCAGC TCCTGCTACA GCGCCTCCTG CTATAGCCCC 
TCCTGTTACA ACGGCAGCAG GTTCGCCAGC CACACCCGCT TCTCCTCCGT GGACAGCGCC 
AAGATCTCTG AGAGCACCGT CTTCTCGTCG CAAGACGATG AGGAGGAGGA GAACAGTGCG 
TTCGAATCTG TGCCCGACTC GGTACAGAGC CCGGAGCTGG ACCCGGAGTC GGCAAATGGC 
GCCGGGCCGT GGCACGACGA GCTGGCCGCC CCCGCCGCCA GCGTGGCAAG AACCGTGGAA 
GGTCTGGAAT CCCCCGTGGC AGGGCCAAGC AATCGGAGAG AAGGTGAATG TCCTATACTC 
CATAATTCCC AGCCAGTAAG CCAGCTTCCT TCCTTGAGGC CTGAACATCA TCACTACCCA 
ACAATCGATG AGCCTCTTCC ACCAAACTGG GAAGCTCGCA TTGACAGCCA CGGGCGGGTC 
TTTTATGTGG ACCACGTGAA CCGCACGACC ACCTGGCAGC GGCCGACAGC AGCAGCCACC 
CCCGACGGGA TGCGGAGATC GGGGTCCATC CAGCAGATGG AGCAACTCAA CAGGCGGTAT 
CAAAATATTC AGCGAACCAT TGCAACAGAG AGACCAGAAG AAGATTCTGG AGGCCAAAGT 
TGTGAGCAGG TCCCAACAGG AGGCAGTGGA GGCGGTGGGA GCGACTCAGA GGCTGACTCT 
TCTCAATCAA GCTTAGATCT GAGGAGAGAA GGGTCACTTT CTCCAGTGAA CTCACAAAAA 
ATCACCTTGT TGCTGCAGTC CCCAGCAGTG AAGTTCATTA CCAATCCCGA GTTTTTCACT 
GTACTACACG CCAATTATGG TGCCTACCGA GTCTTCACCA GTAGCACCTG CTTAAAGCAC 
ATGATTCTGA AAGTCCGGCG GGACGCTCGC AATTTCGAAC GCTACCAGCA CAACCGTGAC 
TTGGTGAATT TCATCAACAT GTTTGCAGAC ACACGGCTGG AATTGCCCCG GGGCTGGGAG 
ATCAAAACGG ACCAGCAGGG AAAGTCTTTT TTCGTGGACC ACAATAGTCG AGCCACCACT 
TTCATTGACC CCCGAATCCC TCTTCAGAAC GGTCGTCTTC CCAATCACCT GACACATCGA 
CAGCACCTCC AGAGGCTCCG GAGTTACAGC GCTGGAGAGG CTTCAGAAGT CTCTCGAAAC 
AGAGGAGCCT CTTTACTGGC CAGGCCAGGA CACAGCCTGG TAGCTGCTAT TCGAAGCCAA 
CATCAACATG AGTCATTGCC ACTAGCATAT AATGACAAGA TTGTGGCATT TCTCCGCCAG 
CCAAACATTT TTGAAATGCT GCAAGAGCGT CAGCCAAGCC TGGCGAGAAA CCACACACTC 
AGGGAGAAAA TCCATTACAT TCGGACTGAG GGTAATCATG GGCTTGAAAA GTTGTCCTGT 
GATGCAGATC TAGTCATTTT GCTGAGTCTC TTTGAAGAAG AGATTATGTC CTACGTCCCA 
CTGCAGACTG CCTTTCATCC CGGGTACAGC TTCTCTCCCC GCTGTTCACC CTGTTCCTCA 
CCTCAGAACT CCCCAGGTTT ACAGAGAGCC AGTGCAAGAG CCCCTTCACC CTACCGGAGA 
GACTTCGAGG CCAAGCTCCG CAATTTCTAC CGAAAACTGG AAGCCAAAGG ATTTGGTCAG 
GGTCCAGGGA AAATTAAGCT CATCATTCGT CGGGACCACT TGTTAGAAGG AACCTTCAAT 
CAGGTGATGG CCTATTCCCG GAAGGAGCTT CAGCGAAACA AACTCTACGT TACCTTTGTT 
GGAGAGGAGG GTCTGGACTA CAGTGGTCCT TCGCGAGAGT TCTTCTTCCT TTTGTCTCAG 
GAGCTCTTCA ACCCTTACTA CGGACTCTTT GAGTACTCTG CAAATGATAC GTACACGGTG 
CAGATCAGCC CCATGTCGGC ATTTGTGGAA AACCATCTTG AATGGTTCAG GTTTAGTGGT 
CGGATCCTAG GCCTGGCTTT GATCCATCAG TACCTTCTTG ATGCCTTCTT CACAAGGCCC 
TTCTATAAGG CACTCCTGAG ACTGCCCTGT GATTTAAGTG ATCTGGAATA TCTGGATGAG 
GAATTTCACC AGAGTTTGCA ATGGATGAAG GACAACAACA TCACAGATAT CCTAGACCTC 
ACTTTCACTG TTAACGAAGA AGTTTTTGGA CAGGTAACGG AAAGGGAGTT GAAATCTGGA 
GGAGCCAACA CGCAGGTGAC GGAGAAGAAC AAGAAGGAGT ACATCGAGAG GATGGTGAAG 
TGGCGGGTGG AGCGCGGTGT GGTGCAGCAG ACCGAGGCCC TGGTGCGGGG CTTCTATGAG 
GTGGTAGACT CGAGGCTTGT CTCTGTGTTC GACGCCAGGG AGCTGGAGCT GGTGATAGCA 
GGCACGGCGG AAATCGACCT GAATGACTGG CGGAATAACA CAGAGTACCG GGGAGGTTAT 
CATGACGGGC ATCTTGTGAT CCGCTGGTTC TGGGCCGCCG TGGAGCGCTT CAATAATGAG 
CAGAGACTGA GATTACTTCA GTTCGTCACG GGAACGTCAA GCGTGCCCTA CGAAGGCTTC 
GCAGCCCTTC GAGGAAGCAA TGGGCTTCGG CGTTTCTGCA TAGAGAAGTG GGGGAAGATT 
ACGTCTCTCC CCAGGGCACA CACATGCTTC AACCGACTGG ATCTTCCACC TTATCCCTCA 
TACTCCATGT TATATGAAAA GCTGCTAACA GCAGTAGAAG AAACCAGCAC CTTTGGACTT 
GAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_026927396.1
CDS	119..4864
Translation	MASPSRNSQNRRRCKEPLRHSYNPGQFHSMAIRTGPHGTVTIPRSTSDTDLVTSDSRSTLMVSSSYYSIGHSQDLVIHWDIKEEVDAGDWIGMYLIDEVLSENFLDYKNRGVNGSHRGQIIWKIDASSYFVEPETKICFKYYHGVSGALRATTPSVTVKNSAAPIFKSIGSDETVQGQGSRRLISFSLSDFQAMGLKKGMFFNPDPYLKISIQPGKHSIFPALPHHGQERRSKIIGNTVNPIWQAEQFSFVSLPTDVLEIEVKDKFAKSRPIIKRFLGKLSMPVQRLLERHAIGDRVVSYTLGRRLPTDHVSGQLQFRFEITSSIHPDDEEMSLSAEPESADIQDSPMNSLLASSRGEPPGEAPELPRGSKPEPPSQGSAGDAAGNGSLELAEPVEEAAGTNEAGDDGAGSVGPDGAGELLGPGREDAEPALSAEELAERLELGGEVPPPGLLEDSEGPGGTWEPEAEEEATESRAGGEEEKEQAEEEEEGDVSALGQGEDRLQLRASVKRKSRPCSLPVSELETVIASACGDPETPRTHYIRIHTLLHSMPSAQGGGAASDDGAEEEATLQDASEKDGLSEVDTLAAAPPPLEEDGEELEGATPGTATPGHSGLTNGLGPDGDTLPSTGSESDSSPRQGGDHSCEGCDTSCCSPSCYSSSCYSTSCYSSSCYSASCYSPSCYNGSRFASHTRFSSVDSAKISESTVFSSQDDEEEENSAFESVPDSVQSPELDPESANGAGPWHDELAAPAASVARTVEGLESPVAGPSNRREGECPILHNSQPVSQLPSLRPEHHHYPTIDEPLPPNWEARIDSHGRVFYVDHVNRTTTWQRPTAAATPDGMRRSGSIQQMEQLNRRYQNIQRTIATERPEEDSGGQSCEQVPTGGSGGGGSDSEADSSQSSLDLRREGSLSPVNSQKITLLLQSPAVKFITNPEFFTVLHANYGAYRVFTSSTCLKHMILKVRRDARNFERYQHNRDLVNFINMFADTRLELPRGWEIKTDQQGKSFFVDHNSRATTFIDPRIPLQNGRLPNHLTHRQHLQRLRSYSAGEASEVSRNRGASLLARPGHSLVAAIRSQHQHESLPLAYNDKIVAFLRQPNIFEMLQERQPSLARNHTLREKIHYIRTEGNHGLEKLSCDADLVILLSLFEEEIMSYVPLQTAFHPGYSFSPRCSPCSSPQNSPGLQRASARAPSPYRRDFEAKLRNFYRKLEAKGFGQGPGKIKLIIRRDHLLEGTFNQVMAYSRKELQRNKLYVTFVGEEGLDYSGPSREFFFLLSQELFNPYYGLFEYSANDTYTVQISPMSAFVENHLEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRLPCDLSDLEYLDEEFHQSLQWMKDNNITDILDLTFTVNEEVFGQVTERELKSGGANTQVTEKNKKEYIERMVKWRVERGVVQQTEALVRGFYEVVDSRLVSVFDARELELVIAGTAEIDLNDWRNNTEYRGGYHDGHLVIRWFWAAVERFNNEQRLRLLQFVTGTSSVPYEGFAALRGSNGLRRFCIEKWGKITSLPRAHTCFNRLDLPPYPSYSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_027071595.1
Organism	Acinonyx jubatus(cheetah)
Definition	Acinonyx jubatus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_027071595.1

ORF Insert Sequence:

ATGGCATCCC CATCTAGAAA CTCCCAGAAT CGCCGCCGGT GCAAGGAGCC ACTCCGTCAC 
AGCTACAACC CTGGCCAGTT CCACAGCATG GCCATCAGGA CCGGCCCCCA CGGCACCGTC 
ACCATACCCC GCTCCACCAG CGACACCGAC CTGGTCACCT CAGACAGCCG CTCCACACTT 
ATGGTCAGCA GCTCCTACTA CTCCATAGGA CACTCTCAGG ACCTGGTGAT CCACTGGGAC 
ATCAAGGAGG AAGTGGATGC TGGAGACTGG ATTGGCATGT ATCTCATTGA TGAGGTCTTG 
TCAGAAAACT TTTTGGACTA TAAGAACCGT GGAGTCAATG GTTCTCATCG GGGCCAGATC 
ATCTGGAAGA TTGATGCCAG CTCTTACTTT GTGGAACCCG AAACTAAGAT CTGCTTCAAA 
TACTACCATG GCGTGAGTGG AGCCCTGCGA GCCACCACCC CCAGTGTCAC GGTCAAAAAC 
TCTGCAGCTC CTATTTTTAA AAGCATTGGC TCTGATGAGA CCGTCCAAGG GCAAGGAAGT 
CGGAGGCTGA TCAGCTTTTC TCTCTCAGAT TTCCAGGCCA TGGGGCTGAA GAAAGGGATG 
TTTTTCAACC CAGACCCTTA CCTGAAGATT TCCATTCAGC CTGGAAAGCA CAGCATCTTC 
CCTGCCCTCC CACACCATGG ACAGGAGAGG AGATCCAAGA TCATAGGCAA CACTGTGAAC 
CCCATCTGGC AGGCCGAGCA ATTCAGCTTT GTGTCCTTGC CCACTGATGT ACTGGAAATT 
GAGGTGAAAG ACAAGTTTGC TAAGAGCCGC CCCATCATCA AGCGCTTCTT GGGAAAGCTG 
TCGATGCCCG TTCAAAGACT CCTGGAGAGA CACGCCATAG GGGATAGGGT GGTCAGCTAT 
ACACTTGGCC GCAGGCTTCC AACAGATCAT GTGAGTGGAC AGCTGCAATT CCGATTTGAG 
ATCACTTCTT CCATCCACCC AGATGATGAG GAGATGTCCC TCAGCGCCGA GCCTGAGTCC 
GCGGACATTC AGGACAGCCC CATGAACAGC CTGTTGGCCA GCAGCCGTGG GGAGCCTCCC 
GGCGAGGCAC CGGAGCTCCC CAGGGGCTCC AAGCCCGAGC CGCCGAGCCA GGGCAGCGCC 
GGGGACGCTG CCGGGAACGG GAGCCTGGAG CTGGCCGAGC CGGTTGAGGA AGCGGCAGGT 
ACCAACGAGG CAGGAGACGA CGGCGCGGGC TCTGTGGGAC CTGACGGGGC TGGGGAGCTT 
CTGGGCCCGG GGCGTGAGGA CGCCGAGCCC GCCCTGAGTG CGGAAGAACT GGCGGAGCGG 
CTGGAGCTGG GTGGGGAGGT GCCCCCACCG GGGCTGCTGG AGGACAGCGA AGGCCCGGGC 
GGCACCTGGG AGCCCGAGGC AGAGGAAGAG GCCACAGAGA GCCGGGCGGG GGGAGAGGAG 
GAGAAGGAGC AGGCGGAGGA GGAGGAGGAG GGCGACGTGT CCGCCCTGGG GCAGGGGGAG 
GACAGGCTGC AGCTGCGGGC CTCGGTGAAG AGGAAAAGCA GGCCGTGCTC CTTGCCGGTG 
TCCGAGCTGG AGACGGTGAT CGCGTCCGCC TGCGGAGACC CCGAGACCCC GCGGACACAC 
TACATCCGCA TCCACACCCT GCTGCACAGC ATGCCCTCCG CCCAGGGCGG CGGCGCCGCG 
AGCGACGACG GCGCCGAGGA GGAGGCCACC CTCCAGGACG CCTCGGAGAA GGACGGGCTC 
AGCGAGGTGG ACACGCTAGC TGCCGCGCCG CCTCCCCTCG AAGAGGATGG GGAGGAACTC 
GAGGGGGCTA CTCCAGGCAC GGCGACCCCC GGCCACTCCG GCCTGACCAA CGGCCTGGGC 
CCGGACGGCG ACACGCTCCC CAGCACAGGG AGTGAGAGCG ACTCGAGTCC CCGGCAGGGT 
GGGGACCACA GCTGCGAGGG CTGTGACACG TCCTGCTGCA GCCCCTCCTG CTACAGCTCC 
TCGTGCTACA GCACGTCCTG CTACAGCAGC TCCTGCTACA GCGCCTCCTG CTATAGCCCC 
TCCTGTTACA ACGGCAGCAG GTTCGCCAGC CACACCCGCT TCTCCTCCGT GGACAGCGCC 
AAGATCTCTG AGAGCACCGT CTTCTCGTCG CAAGACGATG AGGAGGAGGA GAACAGTGCG 
TTCGAATCTG TGCCCGACTC GGTACAGAGC CCGGAGCTGG ACCCGGAGTC GGCAAATGGC 
GCCGGGCCGT GGCACGACGA GCTGGCCGCC CCCGCCGCCA GCGTGGCAAG AACCGTGGAA 
GGTCTGGAAT CCCCCGTGGC AGGGCCAAGC AATCGGAGAG AAGGTGAATG TCCTATACTC 
CATAATTCCC AGCCAGTAAG CCAGCTTCCT TCCTTGAGGC CTGAACATCA TCACTACCCA 
ACAATCGATG AGCCTCTTCC ACCAAACTGG GAAGCTCGCA TTGACAGCCA CGGGCGGGTC 
TTTTATGTGG ACCACGTGAA CCGCACGACC ACCTGGCAGC GGCCGACAGC AGCAGCCACC 
CCCGACGGGA TGCGGAGATC GGGGTCCATC CAGCAGATGG AGCAACTCAA CAGGCGGTAT 
CAAAATATTC AGCGAACCAT TGCAACAGAG AGACCAGAAG AAGATTCTGG AGGCCAAAGT 
TGTGAGCAGG TCCCAACAGG AGGCAGTGGA GGCGGTGGGA GCGACTCAGA GGCTGACTCT 
TCTCAATCAA GCTTAGATCT GAGGAGAGAA GGGTCACTTT CTCCAGTGAA CTCACAAAAA 
ATCACCTTGT TGCTGCAGTC CCCAGCAGTG AAGTTCATTA CCAATCCCGA GTTTTTCACT 
GTACTACACG CCAATTATGG TGCCTACCGA GTCTTCACCA GTAGCACCTG CTTAAAGCAC 
ATGATTCTGA AAGTCCGGCG GGACGCTCGC AATTTCGAAC GCTACCAGCA CAACCGTGAC 
TTGGTGAATT TCATCAACAT GTTTGCAGAC ACACGGCTGG AATTGCCCCG GGGCTGGGAG 
ATCAAAACGG ACCAGCAGGG AAAGTCTTTT TTCGTGGACC ACAATAGTCG AGCCACCACT 
TTCATTGACC CCCGAATCCC TCTTCAGAAC GGTCGTCTTC CCAATCACCT GACACATCGA 
CAGCACCTCC AGAGGCTCCG GAGTTACAGC GCTGGAGAGG CTTCAGAAGT CTCTCGAAAC 
AGAGGAGCCT CTTTACTGGC CAGGCCAGGA CACAGCCTGG TAGCTGCTAT TCGAAGCCAA 
CATCAACATG AGTCATTGCC ACTAGCATAT AATGACAAGA TTGTGGCATT TCTCCGCCAG 
CCAAACATTT TTGAAATGCT GCAAGAGCGT CAGCCAAGCC TGGCGAGAAA CCACACACTC 
AGGGAGAAAA TCCATTACAT TCGGACTGAG GGTAATCATG GGCTTGAAAA GTTGTCCTGT 
GATGCAGATC TAGTCATTTT GCTGAGTCTC TTTGAAGAAG AGATTATGTC CTACGTCCCA 
CTGCAGACTG CCTTTCATCC CGGGTACAGC TTCTCTCCCC GCTGTTCACC CTGTTCCTCA 
CCTCAGAACT CCCCAGGTTT ACAGAGAGCC AGTGCAAGAG CCCCTTCACC CTACCGGAGA 
GACTTCGAGG CCAAGCTCCG CAATTTCTAC CGAAAACTGG AAGCCAAAGG ATTTGGTCAG 
GGTCCAGGGA AAATTAAGCT CATCATTCGT CGGGACCACT TGTTAGAAGG AACCTTCAAT 
CAGGTGATGG CCTATTCCCG GAAGGAGCTT CAGCGAAACA AACTCTACGT TACCTTTGTT 
GGAGAGGAGG GTCTGGACTA CAGTGGTCCT TCGCGAGAGT TCTTCTTCCT TTTGTCTCAG 
GAGCTCTTCA ACCCTTACTA CGGACTCTTT GAGTACTCTG CAAATGATAC GTACACGGTG 
CAGATCAGCC CCATGTCGGC ATTTGTGGAA AACCATCTTG AATGGTTCAG GTTTAGTGGT 
CGGATCCTAG GCCTGGCTTT GATCCATCAG TACCTTCTTG ATGCCTTCTT CACAAGGCCC 
TTCTATAAGG CACTCCTGAG ACTGCCCTGT GATTTAAGTG ATCTGGAATA TCTGGATGAG 
GAATTTCACC AGAGTTTGCA ATGGATGAAG GACAACAACA TCACAGATAT CCTAGACCTC 
ACTTTCACTG TTAACGAAGA AGTTTTTGGA CAGGTAACGG AAAGGGAGTT GAAATCTGGA 
GGAGCCAACA CGCAGGTGAC GGAGAAGAAC AAGAAGGAGT ACATCGAGAG GATGGTGAAG 
TGGCGGGTGG AGCGCGGTGT GGTGCAGCAG ACCGAGGCCC TGGTGCGGGG CTTCTATGAG 
GTGGTAGACT CGAGGCTTGT CTCTGTGTTC GACGCCAGGG AGCTGGAGCT GGTGATAGCA 
GGCACGGCGG AAATCGACCT GAATGACTGG CGGAATAACA CAGAGTACCG GGGAGGTTAT 
CATGACGGGC ATCTTGTGAT CCGCTGGTTC TGGGCCGCCG TGGAGCGCTT CAATAATGAG 
CAGAGACTGA GATTACTTCA GTTCGTCACG GGAACGTCAA GCGTGCCCTA CGAAGGCTTC 
GCAGCCCTTC GAGGAAGCAA TGGGCTTCGG CGTTTCTGCA TAGAGAAGTG GGGGAAGATT 
ACGTCTCTCC CCAGGGCACA CACATGCTTC AACCGACTGG ATCTTCCACC TTATCCCTCA 
TACTCCATGT TATATGAAAA GCTGCTAACA GCAGTAGAAG AAACCAGCAC CTTTGGACTT 
GAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HECW1 ( XM_027071596.1 ) cDNA ORF clone, Acinonyx jubatus(cheetah) -> XP_026927397.1 Acinonyx jubatus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X5, mRNA.

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CloneID	OAc228960
Clone ID Related Accession (Same CDS sequence)	XM_027071595.1 , XM_027071596.1
Accession Version	XM_027071596.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4746bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-12
Organism	Acinonyx jubatus(cheetah)
Product	E3 ubiquitin-protein ligase HECW1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_020834755.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Acinonyx jubatus Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCATCCC CATCTAGAAA CTCCCAGAAT CGCCGCCGGT GCAAGGAGCC ACTCCGTCAC 
AGCTACAACC CTGGCCAGTT CCACAGCATG GCCATCAGGA CCGGCCCCCA CGGCACCGTC 
ACCATACCCC GCTCCACCAG CGACACCGAC CTGGTCACCT CAGACAGCCG CTCCACACTT 
ATGGTCAGCA GCTCCTACTA CTCCATAGGA CACTCTCAGG ACCTGGTGAT CCACTGGGAC 
ATCAAGGAGG AAGTGGATGC TGGAGACTGG ATTGGCATGT ATCTCATTGA TGAGGTCTTG 
TCAGAAAACT TTTTGGACTA TAAGAACCGT GGAGTCAATG GTTCTCATCG GGGCCAGATC 
ATCTGGAAGA TTGATGCCAG CTCTTACTTT GTGGAACCCG AAACTAAGAT CTGCTTCAAA 
TACTACCATG GCGTGAGTGG AGCCCTGCGA GCCACCACCC CCAGTGTCAC GGTCAAAAAC 
TCTGCAGCTC CTATTTTTAA AAGCATTGGC TCTGATGAGA CCGTCCAAGG GCAAGGAAGT 
CGGAGGCTGA TCAGCTTTTC TCTCTCAGAT TTCCAGGCCA TGGGGCTGAA GAAAGGGATG 
TTTTTCAACC CAGACCCTTA CCTGAAGATT TCCATTCAGC CTGGAAAGCA CAGCATCTTC 
CCTGCCCTCC CACACCATGG ACAGGAGAGG AGATCCAAGA TCATAGGCAA CACTGTGAAC 
CCCATCTGGC AGGCCGAGCA ATTCAGCTTT GTGTCCTTGC CCACTGATGT ACTGGAAATT 
GAGGTGAAAG ACAAGTTTGC TAAGAGCCGC CCCATCATCA AGCGCTTCTT GGGAAAGCTG 
TCGATGCCCG TTCAAAGACT CCTGGAGAGA CACGCCATAG GGGATAGGGT GGTCAGCTAT 
ACACTTGGCC GCAGGCTTCC AACAGATCAT GTGAGTGGAC AGCTGCAATT CCGATTTGAG 
ATCACTTCTT CCATCCACCC AGATGATGAG GAGATGTCCC TCAGCGCCGA GCCTGAGTCC 
GCGGACATTC AGGACAGCCC CATGAACAGC CTGTTGGCCA GCAGCCGTGG GGAGCCTCCC 
GGCGAGGCAC CGGAGCTCCC CAGGGGCTCC AAGCCCGAGC CGCCGAGCCA GGGCAGCGCC 
GGGGACGCTG CCGGGAACGG GAGCCTGGAG CTGGCCGAGC CGGTTGAGGA AGCGGCAGGT 
ACCAACGAGG CAGGAGACGA CGGCGCGGGC TCTGTGGGAC CTGACGGGGC TGGGGAGCTT 
CTGGGCCCGG GGCGTGAGGA CGCCGAGCCC GCCCTGAGTG CGGAAGAACT GGCGGAGCGG 
CTGGAGCTGG GTGGGGAGGT GCCCCCACCG GGGCTGCTGG AGGACAGCGA AGGCCCGGGC 
GGCACCTGGG AGCCCGAGGC AGAGGAAGAG GCCACAGAGA GCCGGGCGGG GGGAGAGGAG 
GAGAAGGAGC AGGCGGAGGA GGAGGAGGAG GGCGACGTGT CCGCCCTGGG GCAGGGGGAG 
GACAGGCTGC AGCTGCGGGC CTCGGTGAAG AGGAAAAGCA GGCCGTGCTC CTTGCCGGTG 
TCCGAGCTGG AGACGGTGAT CGCGTCCGCC TGCGGAGACC CCGAGACCCC GCGGACACAC 
TACATCCGCA TCCACACCCT GCTGCACAGC ATGCCCTCCG CCCAGGGCGG CGGCGCCGCG 
AGCGACGACG GCGCCGAGGA GGAGGCCACC CTCCAGGACG CCTCGGAGAA GGACGGGCTC 
AGCGAGGTGG ACACGCTAGC TGCCGCGCCG CCTCCCCTCG AAGAGGATGG GGAGGAACTC 
GAGGGGGCTA CTCCAGGCAC GGCGACCCCC GGCCACTCCG GCCTGACCAA CGGCCTGGGC 
CCGGACGGCG ACACGCTCCC CAGCACAGGG AGTGAGAGCG ACTCGAGTCC CCGGCAGGGT 
GGGGACCACA GCTGCGAGGG CTGTGACACG TCCTGCTGCA GCCCCTCCTG CTACAGCTCC 
TCGTGCTACA GCACGTCCTG CTACAGCAGC TCCTGCTACA GCGCCTCCTG CTATAGCCCC 
TCCTGTTACA ACGGCAGCAG GTTCGCCAGC CACACCCGCT TCTCCTCCGT GGACAGCGCC 
AAGATCTCTG AGAGCACCGT CTTCTCGTCG CAAGACGATG AGGAGGAGGA GAACAGTGCG 
TTCGAATCTG TGCCCGACTC GGTACAGAGC CCGGAGCTGG ACCCGGAGTC GGCAAATGGC 
GCCGGGCCGT GGCACGACGA GCTGGCCGCC CCCGCCGCCA GCGTGGCAAG AACCGTGGAA 
GGTCTGGAAT CCCCCGTGGC AGGGCCAAGC AATCGGAGAG AAGGTGAATG TCCTATACTC 
CATAATTCCC AGCCAGTAAG CCAGCTTCCT TCCTTGAGGC CTGAACATCA TCACTACCCA 
ACAATCGATG AGCCTCTTCC ACCAAACTGG GAAGCTCGCA TTGACAGCCA CGGGCGGGTC 
TTTTATGTGG ACCACGTGAA CCGCACGACC ACCTGGCAGC GGCCGACAGC AGCAGCCACC 
CCCGACGGGA TGCGGAGATC GGGGTCCATC CAGCAGATGG AGCAACTCAA CAGGCGGTAT 
CAAAATATTC AGCGAACCAT TGCAACAGAG AGACCAGAAG AAGATTCTGG AGGCCAAAGT 
TGTGAGCAGG TCCCAACAGG AGGCAGTGGA GGCGGTGGGA GCGACTCAGA GGCTGACTCT 
TCTCAATCAA GCTTAGATCT GAGGAGAGAA GGGTCACTTT CTCCAGTGAA CTCACAAAAA 
ATCACCTTGT TGCTGCAGTC CCCAGCAGTG AAGTTCATTA CCAATCCCGA GTTTTTCACT 
GTACTACACG CCAATTATGG TGCCTACCGA GTCTTCACCA GTAGCACCTG CTTAAAGCAC 
ATGATTCTGA AAGTCCGGCG GGACGCTCGC AATTTCGAAC GCTACCAGCA CAACCGTGAC 
TTGGTGAATT TCATCAACAT GTTTGCAGAC ACACGGCTGG AATTGCCCCG GGGCTGGGAG 
ATCAAAACGG ACCAGCAGGG AAAGTCTTTT TTCGTGGACC ACAATAGTCG AGCCACCACT 
TTCATTGACC CCCGAATCCC TCTTCAGAAC GGTCGTCTTC CCAATCACCT GACACATCGA 
CAGCACCTCC AGAGGCTCCG GAGTTACAGC GCTGGAGAGG CTTCAGAAGT CTCTCGAAAC 
AGAGGAGCCT CTTTACTGGC CAGGCCAGGA CACAGCCTGG TAGCTGCTAT TCGAAGCCAA 
CATCAACATG AGTCATTGCC ACTAGCATAT AATGACAAGA TTGTGGCATT TCTCCGCCAG 
CCAAACATTT TTGAAATGCT GCAAGAGCGT CAGCCAAGCC TGGCGAGAAA CCACACACTC 
AGGGAGAAAA TCCATTACAT TCGGACTGAG GGTAATCATG GGCTTGAAAA GTTGTCCTGT 
GATGCAGATC TAGTCATTTT GCTGAGTCTC TTTGAAGAAG AGATTATGTC CTACGTCCCA 
CTGCAGACTG CCTTTCATCC CGGGTACAGC TTCTCTCCCC GCTGTTCACC CTGTTCCTCA 
CCTCAGAACT CCCCAGGTTT ACAGAGAGCC AGTGCAAGAG CCCCTTCACC CTACCGGAGA 
GACTTCGAGG CCAAGCTCCG CAATTTCTAC CGAAAACTGG AAGCCAAAGG ATTTGGTCAG 
GGTCCAGGGA AAATTAAGCT CATCATTCGT CGGGACCACT TGTTAGAAGG AACCTTCAAT 
CAGGTGATGG CCTATTCCCG GAAGGAGCTT CAGCGAAACA AACTCTACGT TACCTTTGTT 
GGAGAGGAGG GTCTGGACTA CAGTGGTCCT TCGCGAGAGT TCTTCTTCCT TTTGTCTCAG 
GAGCTCTTCA ACCCTTACTA CGGACTCTTT GAGTACTCTG CAAATGATAC GTACACGGTG 
CAGATCAGCC CCATGTCGGC ATTTGTGGAA AACCATCTTG AATGGTTCAG GTTTAGTGGT 
CGGATCCTAG GCCTGGCTTT GATCCATCAG TACCTTCTTG ATGCCTTCTT CACAAGGCCC 
TTCTATAAGG CACTCCTGAG ACTGCCCTGT GATTTAAGTG ATCTGGAATA TCTGGATGAG 
GAATTTCACC AGAGTTTGCA ATGGATGAAG GACAACAACA TCACAGATAT CCTAGACCTC 
ACTTTCACTG TTAACGAAGA AGTTTTTGGA CAGGTAACGG AAAGGGAGTT GAAATCTGGA 
GGAGCCAACA CGCAGGTGAC GGAGAAGAAC AAGAAGGAGT ACATCGAGAG GATGGTGAAG 
TGGCGGGTGG AGCGCGGTGT GGTGCAGCAG ACCGAGGCCC TGGTGCGGGG CTTCTATGAG 
GTGGTAGACT CGAGGCTTGT CTCTGTGTTC GACGCCAGGG AGCTGGAGCT GGTGATAGCA 
GGCACGGCGG AAATCGACCT GAATGACTGG CGGAATAACA CAGAGTACCG GGGAGGTTAT 
CATGACGGGC ATCTTGTGAT CCGCTGGTTC TGGGCCGCCG TGGAGCGCTT CAATAATGAG 
CAGAGACTGA GATTACTTCA GTTCGTCACG GGAACGTCAA GCGTGCCCTA CGAAGGCTTC 
GCAGCCCTTC GAGGAAGCAA TGGGCTTCGG CGTTTCTGCA TAGAGAAGTG GGGGAAGATT 
ACGTCTCTCC CCAGGGCACA CACATGCTTC AACCGACTGG ATCTTCCACC TTATCCCTCA 
TACTCCATGT TATATGAAAA GCTGCTAACA GCAGTAGAAG AAACCAGCAC CTTTGGACTT 
GAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_026927397.1
CDS	261..5006
Translation	MASPSRNSQNRRRCKEPLRHSYNPGQFHSMAIRTGPHGTVTIPRSTSDTDLVTSDSRSTLMVSSSYYSIGHSQDLVIHWDIKEEVDAGDWIGMYLIDEVLSENFLDYKNRGVNGSHRGQIIWKIDASSYFVEPETKICFKYYHGVSGALRATTPSVTVKNSAAPIFKSIGSDETVQGQGSRRLISFSLSDFQAMGLKKGMFFNPDPYLKISIQPGKHSIFPALPHHGQERRSKIIGNTVNPIWQAEQFSFVSLPTDVLEIEVKDKFAKSRPIIKRFLGKLSMPVQRLLERHAIGDRVVSYTLGRRLPTDHVSGQLQFRFEITSSIHPDDEEMSLSAEPESADIQDSPMNSLLASSRGEPPGEAPELPRGSKPEPPSQGSAGDAAGNGSLELAEPVEEAAGTNEAGDDGAGSVGPDGAGELLGPGREDAEPALSAEELAERLELGGEVPPPGLLEDSEGPGGTWEPEAEEEATESRAGGEEEKEQAEEEEEGDVSALGQGEDRLQLRASVKRKSRPCSLPVSELETVIASACGDPETPRTHYIRIHTLLHSMPSAQGGGAASDDGAEEEATLQDASEKDGLSEVDTLAAAPPPLEEDGEELEGATPGTATPGHSGLTNGLGPDGDTLPSTGSESDSSPRQGGDHSCEGCDTSCCSPSCYSSSCYSTSCYSSSCYSASCYSPSCYNGSRFASHTRFSSVDSAKISESTVFSSQDDEEEENSAFESVPDSVQSPELDPESANGAGPWHDELAAPAASVARTVEGLESPVAGPSNRREGECPILHNSQPVSQLPSLRPEHHHYPTIDEPLPPNWEARIDSHGRVFYVDHVNRTTTWQRPTAAATPDGMRRSGSIQQMEQLNRRYQNIQRTIATERPEEDSGGQSCEQVPTGGSGGGGSDSEADSSQSSLDLRREGSLSPVNSQKITLLLQSPAVKFITNPEFFTVLHANYGAYRVFTSSTCLKHMILKVRRDARNFERYQHNRDLVNFINMFADTRLELPRGWEIKTDQQGKSFFVDHNSRATTFIDPRIPLQNGRLPNHLTHRQHLQRLRSYSAGEASEVSRNRGASLLARPGHSLVAAIRSQHQHESLPLAYNDKIVAFLRQPNIFEMLQERQPSLARNHTLREKIHYIRTEGNHGLEKLSCDADLVILLSLFEEEIMSYVPLQTAFHPGYSFSPRCSPCSSPQNSPGLQRASARAPSPYRRDFEAKLRNFYRKLEAKGFGQGPGKIKLIIRRDHLLEGTFNQVMAYSRKELQRNKLYVTFVGEEGLDYSGPSREFFFLLSQELFNPYYGLFEYSANDTYTVQISPMSAFVENHLEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLRLPCDLSDLEYLDEEFHQSLQWMKDNNITDILDLTFTVNEEVFGQVTERELKSGGANTQVTEKNKKEYIERMVKWRVERGVVQQTEALVRGFYEVVDSRLVSVFDARELELVIAGTAEIDLNDWRNNTEYRGGYHDGHLVIRWFWAAVERFNNEQRLRLLQFVTGTSSVPYEGFAALRGSNGLRRFCIEKWGKITSLPRAHTCFNRLDLPPYPSYSMLYEKLLTAVEETSTFGLE

Target ORF information:

RefSeq Version	XM_027071596.1
Organism	Acinonyx jubatus(cheetah)
Definition	Acinonyx jubatus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 1 (HECW1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_027071596.1

ORF Insert Sequence:

ATGGCATCCC CATCTAGAAA CTCCCAGAAT CGCCGCCGGT GCAAGGAGCC ACTCCGTCAC 
AGCTACAACC CTGGCCAGTT CCACAGCATG GCCATCAGGA CCGGCCCCCA CGGCACCGTC 
ACCATACCCC GCTCCACCAG CGACACCGAC CTGGTCACCT CAGACAGCCG CTCCACACTT 
ATGGTCAGCA GCTCCTACTA CTCCATAGGA CACTCTCAGG ACCTGGTGAT CCACTGGGAC 
ATCAAGGAGG AAGTGGATGC TGGAGACTGG ATTGGCATGT ATCTCATTGA TGAGGTCTTG 
TCAGAAAACT TTTTGGACTA TAAGAACCGT GGAGTCAATG GTTCTCATCG GGGCCAGATC 
ATCTGGAAGA TTGATGCCAG CTCTTACTTT GTGGAACCCG AAACTAAGAT CTGCTTCAAA 
TACTACCATG GCGTGAGTGG AGCCCTGCGA GCCACCACCC CCAGTGTCAC GGTCAAAAAC 
TCTGCAGCTC CTATTTTTAA AAGCATTGGC TCTGATGAGA CCGTCCAAGG GCAAGGAAGT 
CGGAGGCTGA TCAGCTTTTC TCTCTCAGAT TTCCAGGCCA TGGGGCTGAA GAAAGGGATG 
TTTTTCAACC CAGACCCTTA CCTGAAGATT TCCATTCAGC CTGGAAAGCA CAGCATCTTC 
CCTGCCCTCC CACACCATGG ACAGGAGAGG AGATCCAAGA TCATAGGCAA CACTGTGAAC 
CCCATCTGGC AGGCCGAGCA ATTCAGCTTT GTGTCCTTGC CCACTGATGT ACTGGAAATT 
GAGGTGAAAG ACAAGTTTGC TAAGAGCCGC CCCATCATCA AGCGCTTCTT GGGAAAGCTG 
TCGATGCCCG TTCAAAGACT CCTGGAGAGA CACGCCATAG GGGATAGGGT GGTCAGCTAT 
ACACTTGGCC GCAGGCTTCC AACAGATCAT GTGAGTGGAC AGCTGCAATT CCGATTTGAG 
ATCACTTCTT CCATCCACCC AGATGATGAG GAGATGTCCC TCAGCGCCGA GCCTGAGTCC 
GCGGACATTC AGGACAGCCC CATGAACAGC CTGTTGGCCA GCAGCCGTGG GGAGCCTCCC 
GGCGAGGCAC CGGAGCTCCC CAGGGGCTCC AAGCCCGAGC CGCCGAGCCA GGGCAGCGCC 
GGGGACGCTG CCGGGAACGG GAGCCTGGAG CTGGCCGAGC CGGTTGAGGA AGCGGCAGGT 
ACCAACGAGG CAGGAGACGA CGGCGCGGGC TCTGTGGGAC CTGACGGGGC TGGGGAGCTT 
CTGGGCCCGG GGCGTGAGGA CGCCGAGCCC GCCCTGAGTG CGGAAGAACT GGCGGAGCGG 
CTGGAGCTGG GTGGGGAGGT GCCCCCACCG GGGCTGCTGG AGGACAGCGA AGGCCCGGGC 
GGCACCTGGG AGCCCGAGGC AGAGGAAGAG GCCACAGAGA GCCGGGCGGG GGGAGAGGAG 
GAGAAGGAGC AGGCGGAGGA GGAGGAGGAG GGCGACGTGT CCGCCCTGGG GCAGGGGGAG 
GACAGGCTGC AGCTGCGGGC CTCGGTGAAG AGGAAAAGCA GGCCGTGCTC CTTGCCGGTG 
TCCGAGCTGG AGACGGTGAT CGCGTCCGCC TGCGGAGACC CCGAGACCCC GCGGACACAC 
TACATCCGCA TCCACACCCT GCTGCACAGC ATGCCCTCCG CCCAGGGCGG CGGCGCCGCG 
AGCGACGACG GCGCCGAGGA GGAGGCCACC CTCCAGGACG CCTCGGAGAA GGACGGGCTC 
AGCGAGGTGG ACACGCTAGC TGCCGCGCCG CCTCCCCTCG AAGAGGATGG GGAGGAACTC 
GAGGGGGCTA CTCCAGGCAC GGCGACCCCC GGCCACTCCG GCCTGACCAA CGGCCTGGGC 
CCGGACGGCG ACACGCTCCC CAGCACAGGG AGTGAGAGCG ACTCGAGTCC CCGGCAGGGT 
GGGGACCACA GCTGCGAGGG CTGTGACACG TCCTGCTGCA GCCCCTCCTG CTACAGCTCC 
TCGTGCTACA GCACGTCCTG CTACAGCAGC TCCTGCTACA GCGCCTCCTG CTATAGCCCC 
TCCTGTTACA ACGGCAGCAG GTTCGCCAGC CACACCCGCT TCTCCTCCGT GGACAGCGCC 
AAGATCTCTG AGAGCACCGT CTTCTCGTCG CAAGACGATG AGGAGGAGGA GAACAGTGCG 
TTCGAATCTG TGCCCGACTC GGTACAGAGC CCGGAGCTGG ACCCGGAGTC GGCAAATGGC 
GCCGGGCCGT GGCACGACGA GCTGGCCGCC CCCGCCGCCA GCGTGGCAAG AACCGTGGAA 
GGTCTGGAAT CCCCCGTGGC AGGGCCAAGC AATCGGAGAG AAGGTGAATG TCCTATACTC 
CATAATTCCC AGCCAGTAAG CCAGCTTCCT TCCTTGAGGC CTGAACATCA TCACTACCCA 
ACAATCGATG AGCCTCTTCC ACCAAACTGG GAAGCTCGCA TTGACAGCCA CGGGCGGGTC 
TTTTATGTGG ACCACGTGAA CCGCACGACC ACCTGGCAGC GGCCGACAGC AGCAGCCACC 
CCCGACGGGA TGCGGAGATC GGGGTCCATC CAGCAGATGG AGCAACTCAA CAGGCGGTAT 
CAAAATATTC AGCGAACCAT TGCAACAGAG AGACCAGAAG AAGATTCTGG AGGCCAAAGT 
TGTGAGCAGG TCCCAACAGG AGGCAGTGGA GGCGGTGGGA GCGACTCAGA GGCTGACTCT 
TCTCAATCAA GCTTAGATCT GAGGAGAGAA GGGTCACTTT CTCCAGTGAA CTCACAAAAA 
ATCACCTTGT TGCTGCAGTC CCCAGCAGTG AAGTTCATTA CCAATCCCGA GTTTTTCACT 
GTACTACACG CCAATTATGG TGCCTACCGA GTCTTCACCA GTAGCACCTG CTTAAAGCAC 
ATGATTCTGA AAGTCCGGCG GGACGCTCGC AATTTCGAAC GCTACCAGCA CAACCGTGAC 
TTGGTGAATT TCATCAACAT GTTTGCAGAC ACACGGCTGG AATTGCCCCG GGGCTGGGAG 
ATCAAAACGG ACCAGCAGGG AAAGTCTTTT TTCGTGGACC ACAATAGTCG AGCCACCACT 
TTCATTGACC CCCGAATCCC TCTTCAGAAC GGTCGTCTTC CCAATCACCT GACACATCGA 
CAGCACCTCC AGAGGCTCCG GAGTTACAGC GCTGGAGAGG CTTCAGAAGT CTCTCGAAAC 
AGAGGAGCCT CTTTACTGGC CAGGCCAGGA CACAGCCTGG TAGCTGCTAT TCGAAGCCAA 
CATCAACATG AGTCATTGCC ACTAGCATAT AATGACAAGA TTGTGGCATT TCTCCGCCAG 
CCAAACATTT TTGAAATGCT GCAAGAGCGT CAGCCAAGCC TGGCGAGAAA CCACACACTC 
AGGGAGAAAA TCCATTACAT TCGGACTGAG GGTAATCATG GGCTTGAAAA GTTGTCCTGT 
GATGCAGATC TAGTCATTTT GCTGAGTCTC TTTGAAGAAG AGATTATGTC CTACGTCCCA 
CTGCAGACTG CCTTTCATCC CGGGTACAGC TTCTCTCCCC GCTGTTCACC CTGTTCCTCA 
CCTCAGAACT CCCCAGGTTT ACAGAGAGCC AGTGCAAGAG CCCCTTCACC CTACCGGAGA 
GACTTCGAGG CCAAGCTCCG CAATTTCTAC CGAAAACTGG AAGCCAAAGG ATTTGGTCAG 
GGTCCAGGGA AAATTAAGCT CATCATTCGT CGGGACCACT TGTTAGAAGG AACCTTCAAT 
CAGGTGATGG CCTATTCCCG GAAGGAGCTT CAGCGAAACA AACTCTACGT TACCTTTGTT 
GGAGAGGAGG GTCTGGACTA CAGTGGTCCT TCGCGAGAGT TCTTCTTCCT TTTGTCTCAG 
GAGCTCTTCA ACCCTTACTA CGGACTCTTT GAGTACTCTG CAAATGATAC GTACACGGTG 
CAGATCAGCC CCATGTCGGC ATTTGTGGAA AACCATCTTG AATGGTTCAG GTTTAGTGGT 
CGGATCCTAG GCCTGGCTTT GATCCATCAG TACCTTCTTG ATGCCTTCTT CACAAGGCCC 
TTCTATAAGG CACTCCTGAG ACTGCCCTGT GATTTAAGTG ATCTGGAATA TCTGGATGAG 
GAATTTCACC AGAGTTTGCA ATGGATGAAG GACAACAACA TCACAGATAT CCTAGACCTC 
ACTTTCACTG TTAACGAAGA AGTTTTTGGA CAGGTAACGG AAAGGGAGTT GAAATCTGGA 
GGAGCCAACA CGCAGGTGAC GGAGAAGAAC AAGAAGGAGT ACATCGAGAG GATGGTGAAG 
TGGCGGGTGG AGCGCGGTGT GGTGCAGCAG ACCGAGGCCC TGGTGCGGGG CTTCTATGAG 
GTGGTAGACT CGAGGCTTGT CTCTGTGTTC GACGCCAGGG AGCTGGAGCT GGTGATAGCA 
GGCACGGCGG AAATCGACCT GAATGACTGG CGGAATAACA CAGAGTACCG GGGAGGTTAT 
CATGACGGGC ATCTTGTGAT CCGCTGGTTC TGGGCCGCCG TGGAGCGCTT CAATAATGAG 
CAGAGACTGA GATTACTTCA GTTCGTCACG GGAACGTCAA GCGTGCCCTA CGAAGGCTTC 
GCAGCCCTTC GAGGAAGCAA TGGGCTTCGG CGTTTCTGCA TAGAGAAGTG GGGGAAGATT 
ACGTCTCTCC CCAGGGCACA CACATGCTTC AACCGACTGG ATCTTCCACC TTATCCCTCA 
TACTCCATGT TATATGAAAA GCTGCTAACA GCAGTAGAAG AAACCAGCAC CTTTGGACTT 
GAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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03-6811-6572

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オンライン注文

HECW1 cDNA ORF clone, Acinonyx jubatus(cheetah)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム