casr cDNA ORF clone, Takifugu rubripes(torafugu)

Gene
Clones
gRNAs

Summary

Gene Symbol	casr
Entrez Gene ID	101080211
Full Name	calcium sensing receptor
Gene Type	protein-coding
Organism	Takifugu rubripes(torafugu)

mRNA and Protein(s)

mRNA	Protein	Name
XM_003971269.2	XP_003971318.2	extracellular calcium-sensing receptor
XM_003971269.3	XP_003971318.3	extracellular calcium-sensing receptor

Pathways from BioSystems
Gene Ontology

The following casr gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the casr cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OTo32333	XM_003971269.2 Latest version!	Takifugu rubripes calcium-sensing receptor (casr), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OTo50375	XM_003971269.3 Latest version!	Takifugu rubripes calcium sensing receptor (casr), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

casr ( XM_003971269.2 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_003971318.2 Takifugu rubripes calcium-sensing receptor (casr), mRNA.

Price & Availability↑

CloneID	OTo32333
Clone ID Related Accession (Same CDS sequence)	XM_003971269.2
Accession Version	XM_003971269.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2739bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-15
Organism	Takifugu rubripes(Fugu rubripes)
Product	extracellular calcium-sensing receptor
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004071094.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Mar 16, 2015 this sequence version replaced XM_003971269.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Takifugu rubripes Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTGGCG ATATTCTGCT GGGAGGACTT TTCCCCATAC ACTTTGGCAT TTCTTCCAAA 
GACGAAAACC TCGCTGCCCG ACCGGAATCC ACAAAGTGTG TCAGGTTCAA TTTCCGTGGT 
TTCCGCTGGC TGCAGGCCAT GGTTTTCGCC ATCGAGGAGA TCAACAACAG CAGCAGCCTC 
CTGCCCAACA TCACCCTCGG CTACAGGATC TTCGACACGT GCAACACGGT TTCAAAAGCC 
CTGGAGGCTA CGCTGAGCTT CGTGGCCCAA AACAAGATCG ACTCCCTGAA TTTGGATGAA 
TTTTGCAACT GCACGGATCA CATCCCGGCA ACCATCGCGG TGGTGGGAGC GGCAGGGTCT 
GCGGTCTCTA CGGCAGTCGC AAACCTGCTG AGTCTCTTTT ATATTCCTCA GATCAGCTAT 
GCGTCCTCCA GCCGCTTGCT GAGCAACAAG AATCAATACA AGTCCTTCAT GAGAACCATC 
CCTACAGATG AGCACCAGGC CACAGCCATG GCCGACGTCA TCGAGTACTT CCAGTGGAAC 
TGGGTCATTG CCGTGGCCTC TGATGACGAT TATGGGCGGC CTGGGATAGA AAAGTTTGAG 
AAGGAGATGG AAGAGCGAGA CATTTGCATC CATCTGAACG AACTCATCTC TCAGTACTTC 
GAGGACTGTG AAATCAAAGC TCTCGTGGAC AGAATTGAAA ACTCCACAGC CAAAGTCATC 
GTTGTGTTTG CCAGCGGCCC TGATATCGAG CCTCTGATCA AAGAAATGGT GAGGAGGAAC 
ATCACCGATC GGATCTGGTT AGCCAGTGAA GCGTGGGCAA GCTCCTCCCT CATCGCTAAA 
CCAGAATATC TCGATGTTGT GGAGGGCACA ATCGGCTTTG TTTTGAAAGC AGGGAACATA 
CCTGGTTTCA GGGAGTTCTT ACAGCAGGTC CAGCCAAAGA GAGGCAGCCA CAACGAATTT 
GTCAGGGAGT TTTGGGAGGA AACCTTCAAC TGTTACCTGG AGGACAGCCC GAGACTGCAA 
GAAAGTGAGA ATGGTAGCGA CAGTTTCAGG CCGCTTTGCA CCAGCGAGGA AGACATCACG 
AGTGTGGAGA CCCCGTACCT CGACCACACA CACCTCCGTA TCTCCTACAA TGTCTATGTT 
GCAGTTTACT CCATCGCTCA CGCCCTCCAG GACATACTCT CCTGCACTCC TGGACATGGA 
CTCTTTGCTA ACAACTCTTG CGCGGATATA AAGAAAATGG AAGCATGGCA GGTCCTGAAG 
CAGCTCAGAC ATTTGAACTA CACCAACAGC ATGGGGGAAA AGGTTCATTT TGATGAGAAC 
GCAGACATGG AAGCAAACTA CACCATCATA AACTGGCACC GGTCTGCTGA GGACGGCTCG 
GTGGCGTTCA GGGAGGTGGG CTACTACCAC ATGCACGCCA GGCGGGGAGC CAAACTGCTC 
ATTGATAACA CAAAGATGAT GTGGAATGCA TACAGTTCAG AGGTGCCGTT TTCTAACTGC 
AGCGAGGACT GCGAACCTGG GACGAGGAAG GGCATCATTG ACAGCATGCC CACGTGCTGC 
TTTGAATGTA CCGAGTGCTC AGACGGCGAG TACAGCGATC ATAAAGACGC CAGCATTTGC 
ACCAAGTGTC CAAACAACTC CTGGTCCAGT GGGAACCACA CCTTCTGCTT TCTGAAGGAA 
ATTGAGTTCC TGGCCTGGTC AGAACCCTTT GGGATCGCTT TGGCCATCTG CGCGGTCCTG 
GGGGTTCTCC TGACGGCATT TGTGATGGGA GTCTTTGTCA GATTTCGCAA CACTCCGATC 
GTGAAGGCCT CAAACCGAGA ACTGTCCTAC GTCCTCTTGT TGTCACTCAT CTGCTGTTTT 
TCCAGCTCAC TCATCTTCAT CGGGGAGCCC CAAGACTGGA CCTGCCGTTT ACGCCAGCCT 
GCCTTCGGGA TCAGTTTTGT CCTTTGCATC TCCTGCATCC TCGTGAAAAC CAACAGGGTT 
CTTTTGGTGT TTGAAGCCAA GATCCCCACC AGCATCCATC GTAAATGGTG GGGCCTGAAC 
CTGCAGTTCC TCCTGGTGTT TCTGTGCACC TTCGTTCAGG TCATGATATG TGTGGTCTGG 
CTTTACAACG CCCCCCCCTC CAGCTACAGG AATCATGACA TCGATGAGAT CATTTTCATC 
ACCTGCAATG AGGGATCCGT GATGGCGCTC GGGTTTCTCA TTGGCTACAC GTGCTTACTA 
GCGGCCATAT GCTTCTTCTT TGCGTTTAAG TCAAGGAAAC TTCCCGAAAA CTTCACGGAG 
GCCAAGTTCA TCACTTTCTG CATGCTCATC TTCTTTATTG TTTGGATTTC TTTTATCCCT 
GCGTACTTCA GTACTTACGG CAAGTTTGTT TCAGCGGTAG AGGCCATTGC CATCCTGGCA 
TCCAGCTACG GGATGCTAGC TTGTATTTTC TTTAATAAGG TCTACATCAT CCTGTTCAAA 
CCCTGTAGGA ACACCATAGA AGAGGTCAGG TGCAGCACCG CGGCCCACGC TTTCAGAGTG 
GCCGCCAAAG CTACATTAAA ACACAGAACC ACTGTGAGGA AAAAGTCGAA CAGCATCGGC 
TCTACCGCCT CCACTCCATC ATCATCAATT AGCCTGAAGA CCAACAGCAA CGACTGCGAC 
TCCGCCTCAG GGAGACATAG GCCGAGGGTG AGCTTCGGCA GCGGAACTGT CATGCTGTCC 
TTGAGCTTTG AGGAGTCCAG GAGGAGCTCT CTGATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003971318.2
CDS	1..2739
Translation	MTGDILLGGLFPIHFGISSKDENLAARPESTKCVRFNFRGFRWLQAMVFAIEEINNSSSLLPNITLGYRIFDTCNTVSKALEATLSFVAQNKIDSLNLDEFCNCTDHIPATIAVVGAAGSAVSTAVANLLSLFYIPQISYASSSRLLSNKNQYKSFMRTIPTDEHQATAMADVIEYFQWNWVIAVASDDDYGRPGIEKFEKEMEERDICIHLNELISQYFEDCEIKALVDRIENSTAKVIVVFASGPDIEPLIKEMVRRNITDRIWLASEAWASSSLIAKPEYLDVVEGTIGFVLKAGNIPGFREFLQQVQPKRGSHNEFVREFWEETFNCYLEDSPRLQESENGSDSFRPLCTSEEDITSVETPYLDHTHLRISYNVYVAVYSIAHALQDILSCTPGHGLFANNSCADIKKMEAWQVLKQLRHLNYTNSMGEKVHFDENADMEANYTIINWHRSAEDGSVAFREVGYYHMHARRGAKLLIDNTKMMWNAYSSEVPFSNCSEDCEPGTRKGIIDSMPTCCFECTECSDGEYSDHKDASICTKCPNNSWSSGNHTFCFLKEIEFLAWSEPFGIALAICAVLGVLLTAFVMGVFVRFRNTPIVKASNRELSYVLLLSLICCFSSSLIFIGEPQDWTCRLRQPAFGISFVLCISCILVKTNRVLLVFEAKIPTSIHRKWWGLNLQFLLVFLCTFVQVMICVVWLYNAPPSSYRNHDIDEIIFITCNEGSVMALGFLIGYTCLLAAICFFFAFKSRKLPENFTEAKFITFCMLIFFIVWISFIPAYFSTYGKFVSAVEAIAILASSYGMLACIFFNKVYIILFKPCRNTIEEVRCSTAAHAFRVAAKATLKHRTTVRKKSNSIGSTASTPSSSISLKTNSNDCDSASGRHRPRVSFGSGTVMLSLSFEESRRSSLM

Target ORF information:

RefSeq Version	XM_003971269.2
Organism	Takifugu rubripes(Fugu rubripes)
Definition	Takifugu rubripes calcium-sensing receptor (casr), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003971269.2

ORF Insert Sequence:

ATGACTGGCG ATATTCTGCT GGGAGGACTT TTCCCCATAC ACTTTGGCAT TTCTTCCAAA 
GACGAAAACC TCGCTGCCCG ACCGGAATCC ACAAAGTGTG TCAGGTTCAA TTTCCGTGGT 
TTCCGCTGGC TGCAGGCCAT GGTTTTCGCC ATCGAGGAGA TCAACAACAG CAGCAGCCTC 
CTGCCCAACA TCACCCTCGG CTACAGGATC TTCGACACGT GCAACACGGT TTCAAAAGCC 
CTGGAGGCTA CGCTGAGCTT CGTGGCCCAA AACAAGATCG ACTCCCTGAA TTTGGATGAA 
TTTTGCAACT GCACGGATCA CATCCCGGCA ACCATCGCGG TGGTGGGAGC GGCAGGGTCT 
GCGGTCTCTA CGGCAGTCGC AAACCTGCTG AGTCTCTTTT ATATTCCTCA GATCAGCTAT 
GCGTCCTCCA GCCGCTTGCT GAGCAACAAG AATCAATACA AGTCCTTCAT GAGAACCATC 
CCTACAGATG AGCACCAGGC CACAGCCATG GCCGACGTCA TCGAGTACTT CCAGTGGAAC 
TGGGTCATTG CCGTGGCCTC TGATGACGAT TATGGGCGGC CTGGGATAGA AAAGTTTGAG 
AAGGAGATGG AAGAGCGAGA CATTTGCATC CATCTGAACG AACTCATCTC TCAGTACTTC 
GAGGACTGTG AAATCAAAGC TCTCGTGGAC AGAATTGAAA ACTCCACAGC CAAAGTCATC 
GTTGTGTTTG CCAGCGGCCC TGATATCGAG CCTCTGATCA AAGAAATGGT GAGGAGGAAC 
ATCACCGATC GGATCTGGTT AGCCAGTGAA GCGTGGGCAA GCTCCTCCCT CATCGCTAAA 
CCAGAATATC TCGATGTTGT GGAGGGCACA ATCGGCTTTG TTTTGAAAGC AGGGAACATA 
CCTGGTTTCA GGGAGTTCTT ACAGCAGGTC CAGCCAAAGA GAGGCAGCCA CAACGAATTT 
GTCAGGGAGT TTTGGGAGGA AACCTTCAAC TGTTACCTGG AGGACAGCCC GAGACTGCAA 
GAAAGTGAGA ATGGTAGCGA CAGTTTCAGG CCGCTTTGCA CCAGCGAGGA AGACATCACG 
AGTGTGGAGA CCCCGTACCT CGACCACACA CACCTCCGTA TCTCCTACAA TGTCTATGTT 
GCAGTTTACT CCATCGCTCA CGCCCTCCAG GACATACTCT CCTGCACTCC TGGACATGGA 
CTCTTTGCTA ACAACTCTTG CGCGGATATA AAGAAAATGG AAGCATGGCA GGTCCTGAAG 
CAGCTCAGAC ATTTGAACTA CACCAACAGC ATGGGGGAAA AGGTTCATTT TGATGAGAAC 
GCAGACATGG AAGCAAACTA CACCATCATA AACTGGCACC GGTCTGCTGA GGACGGCTCG 
GTGGCGTTCA GGGAGGTGGG CTACTACCAC ATGCACGCCA GGCGGGGAGC CAAACTGCTC 
ATTGATAACA CAAAGATGAT GTGGAATGCA TACAGTTCAG AGGTGCCGTT TTCTAACTGC 
AGCGAGGACT GCGAACCTGG GACGAGGAAG GGCATCATTG ACAGCATGCC CACGTGCTGC 
TTTGAATGTA CCGAGTGCTC AGACGGCGAG TACAGCGATC ATAAAGACGC CAGCATTTGC 
ACCAAGTGTC CAAACAACTC CTGGTCCAGT GGGAACCACA CCTTCTGCTT TCTGAAGGAA 
ATTGAGTTCC TGGCCTGGTC AGAACCCTTT GGGATCGCTT TGGCCATCTG CGCGGTCCTG 
GGGGTTCTCC TGACGGCATT TGTGATGGGA GTCTTTGTCA GATTTCGCAA CACTCCGATC 
GTGAAGGCCT CAAACCGAGA ACTGTCCTAC GTCCTCTTGT TGTCACTCAT CTGCTGTTTT 
TCCAGCTCAC TCATCTTCAT CGGGGAGCCC CAAGACTGGA CCTGCCGTTT ACGCCAGCCT 
GCCTTCGGGA TCAGTTTTGT CCTTTGCATC TCCTGCATCC TCGTGAAAAC CAACAGGGTT 
CTTTTGGTGT TTGAAGCCAA GATCCCCACC AGCATCCATC GTAAATGGTG GGGCCTGAAC 
CTGCAGTTCC TCCTGGTGTT TCTGTGCACC TTCGTTCAGG TCATGATATG TGTGGTCTGG 
CTTTACAACG CCCCCCCCTC CAGCTACAGG AATCATGACA TCGATGAGAT CATTTTCATC 
ACCTGCAATG AGGGATCCGT GATGGCGCTC GGGTTTCTCA TTGGCTACAC GTGCTTACTA 
GCGGCCATAT GCTTCTTCTT TGCGTTTAAG TCAAGGAAAC TTCCCGAAAA CTTCACGGAG 
GCCAAGTTCA TCACTTTCTG CATGCTCATC TTCTTTATTG TTTGGATTTC TTTTATCCCT 
GCGTACTTCA GTACTTACGG CAAGTTTGTT TCAGCGGTAG AGGCCATTGC CATCCTGGCA 
TCCAGCTACG GGATGCTAGC TTGTATTTTC TTTAATAAGG TCTACATCAT CCTGTTCAAA 
CCCTGTAGGA ACACCATAGA AGAGGTCAGG TGCAGCACCG CGGCCCACGC TTTCAGAGTG 
GCCGCCAAAG CTACATTAAA ACACAGAACC ACTGTGAGGA AAAAGTCGAA CAGCATCGGC 
TCTACCGCCT CCACTCCATC ATCATCAATT AGCCTGAAGA CCAACAGCAA CGACTGCGAC 
TCCGCCTCAG GGAGACATAG GCCGAGGGTG AGCTTCGGCA GCGGAACTGT CATGCTGTCC 
TTGAGCTTTG AGGAGTCCAG GAGGAGCTCT CTGATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

casr ( XM_003971269.3 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_003971318.3 Takifugu rubripes calcium sensing receptor (casr), mRNA.

Price & Availability↑

CloneID	OTo50375
Clone ID Related Accession (Same CDS sequence)	XM_003971269.3
Accession Version	XM_003971269.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2823bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-04
Organism	Takifugu rubripes(torafugu)
Product	extracellular calcium-sensing receptor
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042299.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jul 5, 2019 this sequence version replaced XM_003971269.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCGAC TGGTGCTGCA TTATCTCATA CTTCTGGGGT CCGGTTATGT CATTTCAACA 
TATGGGCCTA ATCAGAGAGC ACAGATGACT GGCGATATTC TGCTGGGAGG ACTTTTCCCC 
ATACACTTTG GCATTTCTTC CAAAGACGAA AACCTCGCTG CCCGACCGGA ATCCACAAAG 
TGTGTCAGGT TCAATTTCCG TGGTTTCCGC TGGCTGCAGG CCATGGTTTT CGCCATCGAG 
GAGATCAACA ACAGCAGCAG CCTCCTGCCC AACATCACCC TCGGCTACAG GATCTTCGAC 
ACGTGCAACA CGGTTTCAAA AGCCCTGGAG GCTACGCTGA GCTTCGTGGC CCAAAACAAG 
ATCGACTCCC TGAATTTGGA TGAATTTTGC AACTGCACGG ATCACATCCC GGCAACCATC 
GCGGTGGTGG GAGCGGCAGG GTCTGCGGTC TCTACGGCAG TCGCAAACCT GCTGAGTCTC 
TTTTATATTC CTCAGATCAG CTATGCGTCC TCCAGCCGCT TGCTGAGCAA CAAGAATCAA 
TACAAGTCCT TCATGAGAAC CATCCCTACA GATGAGCACC AGGCCACAGC CATGGCCGAC 
GTCATCGAGT ACTTCCAGTG GAACTGGGTC ATTGCCGTGG CCTCTGATGA CGATTATGGG 
CGGCCTGGGA TAGAAAAGTT TGAGAAGGAG ATGGAAGAGC GAGACATTTG CATCCATCTG 
AACGAACTCA TCTCTCAGTA CTTCGAGGAC TGTGAAATCA AAGCTCTCGT GGACAGAATT 
GAAAACTCCA CAGCCAAAGT CATCGTTGTG TTTGCCAGCG GCCCTGATAT CGAGCCTCTG 
ATCAAAGAAA TGGTGAGGAG GAACATCACC GATCGGATCT GGTTAGCCAG TGAAGCGTGG 
GCAAGCTCCT CCCTCATCGC TAAACCAGAA TATCTCGATG TTGTGGAGGG CACAATCGGC 
TTTGTTTTGA AAGCAGGGAA CATACCTGGT TTCAGGGAGT TCTTACAGCA GGTCCAGCCA 
AAGAGAGGCA GCCACAACGA ATTTGTCAGG GAGTTTTGGG AGGAAACCTT CAACTGTTAC 
CTGGAGGACA GCCCGAGACT GCAAGAAAGT GAGAATGGTA GCGACAGTTT CAGGCCGCTT 
TGCACCAGCG AGGAAGACAT CACGAGTGTG GAGACCCCGT ACCTCGACCA CACACACCTC 
CGTATCTCCT ACAATGTCTA TGTTGCAGTT TACTCCATCG CTCACGCCCT CCAGGACATA 
CTCTCCTGCA CTCCTGGACA TGGACTCTTT GCTAACAACT CTTGCGCGGA TATAAAGAAA 
ATGGAAGCAT GGCAGGTCCT GAAGCAGCTC AGACATTTGA ACTACACCAA CAGCATGGGG 
GAAAAGGTTC ATTTTGATGA GAACGCAGAC ATGGAAGCAA ACTACACCAT CATAAACTGG 
CACCGGTCTG CTGAGGACGG CTCGGTGGCG TTCAGGGAGG TGGGCTACTA CCACATGCAC 
GCCAGGCGGG GAGCCAAACT GCTCATTGAT AACACAAAGA TGATGTGGAA TGCATACAGT 
TCAGAGGTGC CGTTTTCTAA CTGCAGCGAG GACTGCGAAC CTGGGACGAG GAAGGGCATC 
ATTGACAGCA TGCCCACGTG CTGCTTTGAA TGTACCGAGT GCTCAGACGG CGAGTACAGC 
GATCATAAAG ACGCCAGCAT TTGCACCAAG TGTCCAAACA ACTCCTGGTC CAGTGGGAAC 
CACACCTTCT GCTTTCTGAA GGAAATTGAG TTCCTGGCCT GGTCAGAACC CTTTGGGATC 
GCTTTGGCCA TCTGCGCGGT CCTGGGGGTT CTCCTGACGG CATTTGTGAT GGGAGTCTTT 
GTCAGATTTC GCAACACTCC GATCGTGAAG GCCTCAAACC GAGAACTGTC CTACGTCCTC 
TTGTTGTCAC TCATCTGCTG TTTTTCCAGC TCACTCATCT TCATCGGGGA GCCCCAAGAC 
TGGACCTGCC GTTTACGCCA GCCTGCCTTC GGGATCAGTT TTGTCCTTTG CATCTCCTGC 
ATCCTCGTGA AAACCAACAG GGTTCTTTTG GTGTTTGAAG CCAAGATCCC CACCAGCATC 
CATCGTAAAT GGTGGGGCCT GAACCTGCAG TTCCTCCTGG TGTTTCTGTG CACCTTCGTT 
CAGGTCATGA TATGTGTGGT CTGGCTTTAC AACGCCCCCC CCTCCAGCTA CAGGAATCAT 
GACATCGATG AGATCATTTT CATCACCTGT AATGAGGGAT CCGTGATGGC GCTCGGGTTT 
CTCATTGGCT ACACGTGCTT ACTAGCGGCC ATATGCTTCT TCTTTGCGTT TAAGTCAAGG 
AAACTTCCCG AAAACTTCAC GGAGGCCAAG TTCATCACTT TCTGCATGCT CATCTTCTTT 
ATTGTTTGGA TTTCTTTTAT CCCTGCGTAC TTCAGTACTT ACGGCAAGTT TGTTTCAGCA 
GTAGAGGCCA TTGCCATCCT GGCATCCAGC TACGGGATGC TAGCTTGTAT TTTCTTTAAT 
AAGGTCTACA TCATCCTGTT CAAACCCTGT AGGAACACCA TAGAAGAGGT CAGGTGCAGC 
ACCGCGGCCC ACGCTTTCAG AGTGGCCGCC AAAGCTACAT TAAAACACAG AACCACTGTG 
AGGAAAAAGT CGAACAGCAT CGGCTCTACC GCCTCCACTC CATCATCATC AATTAGCCTG 
AAGACCAACA GCAACGACTG CGACTCCGCC TCAGGGAGGC ATAGGCCGAG GGTGAGCTTC 
GGCAGCGGAA CTGTCATGCT GTCCTTGAGC TTTGAGGAGT CCAGGAGGAG CTCTCTGATG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003971318.3
CDS	266..3088
Translation	MVRLVLHYLILLGSGYVISTYGPNQRAQMTGDILLGGLFPIHFGISSKDENLAARPESTKCVRFNFRGFRWLQAMVFAIEEINNSSSLLPNITLGYRIFDTCNTVSKALEATLSFVAQNKIDSLNLDEFCNCTDHIPATIAVVGAAGSAVSTAVANLLSLFYIPQISYASSSRLLSNKNQYKSFMRTIPTDEHQATAMADVIEYFQWNWVIAVASDDDYGRPGIEKFEKEMEERDICIHLNELISQYFEDCEIKALVDRIENSTAKVIVVFASGPDIEPLIKEMVRRNITDRIWLASEAWASSSLIAKPEYLDVVEGTIGFVLKAGNIPGFREFLQQVQPKRGSHNEFVREFWEETFNCYLEDSPRLQESENGSDSFRPLCTSEEDITSVETPYLDHTHLRISYNVYVAVYSIAHALQDILSCTPGHGLFANNSCADIKKMEAWQVLKQLRHLNYTNSMGEKVHFDENADMEANYTIINWHRSAEDGSVAFREVGYYHMHARRGAKLLIDNTKMMWNAYSSEVPFSNCSEDCEPGTRKGIIDSMPTCCFECTECSDGEYSDHKDASICTKCPNNSWSSGNHTFCFLKEIEFLAWSEPFGIALAICAVLGVLLTAFVMGVFVRFRNTPIVKASNRELSYVLLLSLICCFSSSLIFIGEPQDWTCRLRQPAFGISFVLCISCILVKTNRVLLVFEAKIPTSIHRKWWGLNLQFLLVFLCTFVQVMICVVWLYNAPPSSYRNHDIDEIIFITCNEGSVMALGFLIGYTCLLAAICFFFAFKSRKLPENFTEAKFITFCMLIFFIVWISFIPAYFSTYGKFVSAVEAIAILASSYGMLACIFFNKVYIILFKPCRNTIEEVRCSTAAHAFRVAAKATLKHRTTVRKKSNSIGSTASTPSSSISLKTNSNDCDSASGRHRPRVSFGSGTVMLSLSFEESRRSSLM

Target ORF information:

RefSeq Version	XM_003971269.3
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes calcium sensing receptor (casr), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003971269.3

ORF Insert Sequence:

ATGGTGCGAC TGGTGCTGCA TTATCTCATA CTTCTGGGGT CCGGTTATGT CATTTCAACA 
TATGGGCCTA ATCAGAGAGC ACAGATGACT GGCGATATTC TGCTGGGAGG ACTTTTCCCC 
ATACACTTTG GCATTTCTTC CAAAGACGAA AACCTCGCTG CCCGACCGGA ATCCACAAAG 
TGTGTCAGGT TCAATTTCCG TGGTTTCCGC TGGCTGCAGG CCATGGTTTT CGCCATCGAG 
GAGATCAACA ACAGCAGCAG CCTCCTGCCC AACATCACCC TCGGCTACAG GATCTTCGAC 
ACGTGCAACA CGGTTTCAAA AGCCCTGGAG GCTACGCTGA GCTTCGTGGC CCAAAACAAG 
ATCGACTCCC TGAATTTGGA TGAATTTTGC AACTGCACGG ATCACATCCC GGCAACCATC 
GCGGTGGTGG GAGCGGCAGG GTCTGCGGTC TCTACGGCAG TCGCAAACCT GCTGAGTCTC 
TTTTATATTC CTCAGATCAG CTATGCGTCC TCCAGCCGCT TGCTGAGCAA CAAGAATCAA 
TACAAGTCCT TCATGAGAAC CATCCCTACA GATGAGCACC AGGCCACAGC CATGGCCGAC 
GTCATCGAGT ACTTCCAGTG GAACTGGGTC ATTGCCGTGG CCTCTGATGA CGATTATGGG 
CGGCCTGGGA TAGAAAAGTT TGAGAAGGAG ATGGAAGAGC GAGACATTTG CATCCATCTG 
AACGAACTCA TCTCTCAGTA CTTCGAGGAC TGTGAAATCA AAGCTCTCGT GGACAGAATT 
GAAAACTCCA CAGCCAAAGT CATCGTTGTG TTTGCCAGCG GCCCTGATAT CGAGCCTCTG 
ATCAAAGAAA TGGTGAGGAG GAACATCACC GATCGGATCT GGTTAGCCAG TGAAGCGTGG 
GCAAGCTCCT CCCTCATCGC TAAACCAGAA TATCTCGATG TTGTGGAGGG CACAATCGGC 
TTTGTTTTGA AAGCAGGGAA CATACCTGGT TTCAGGGAGT TCTTACAGCA GGTCCAGCCA 
AAGAGAGGCA GCCACAACGA ATTTGTCAGG GAGTTTTGGG AGGAAACCTT CAACTGTTAC 
CTGGAGGACA GCCCGAGACT GCAAGAAAGT GAGAATGGTA GCGACAGTTT CAGGCCGCTT 
TGCACCAGCG AGGAAGACAT CACGAGTGTG GAGACCCCGT ACCTCGACCA CACACACCTC 
CGTATCTCCT ACAATGTCTA TGTTGCAGTT TACTCCATCG CTCACGCCCT CCAGGACATA 
CTCTCCTGCA CTCCTGGACA TGGACTCTTT GCTAACAACT CTTGCGCGGA TATAAAGAAA 
ATGGAAGCAT GGCAGGTCCT GAAGCAGCTC AGACATTTGA ACTACACCAA CAGCATGGGG 
GAAAAGGTTC ATTTTGATGA GAACGCAGAC ATGGAAGCAA ACTACACCAT CATAAACTGG 
CACCGGTCTG CTGAGGACGG CTCGGTGGCG TTCAGGGAGG TGGGCTACTA CCACATGCAC 
GCCAGGCGGG GAGCCAAACT GCTCATTGAT AACACAAAGA TGATGTGGAA TGCATACAGT 
TCAGAGGTGC CGTTTTCTAA CTGCAGCGAG GACTGCGAAC CTGGGACGAG GAAGGGCATC 
ATTGACAGCA TGCCCACGTG CTGCTTTGAA TGTACCGAGT GCTCAGACGG CGAGTACAGC 
GATCATAAAG ACGCCAGCAT TTGCACCAAG TGTCCAAACA ACTCCTGGTC CAGTGGGAAC 
CACACCTTCT GCTTTCTGAA GGAAATTGAG TTCCTGGCCT GGTCAGAACC CTTTGGGATC 
GCTTTGGCCA TCTGCGCGGT CCTGGGGGTT CTCCTGACGG CATTTGTGAT GGGAGTCTTT 
GTCAGATTTC GCAACACTCC GATCGTGAAG GCCTCAAACC GAGAACTGTC CTACGTCCTC 
TTGTTGTCAC TCATCTGCTG TTTTTCCAGC TCACTCATCT TCATCGGGGA GCCCCAAGAC 
TGGACCTGCC GTTTACGCCA GCCTGCCTTC GGGATCAGTT TTGTCCTTTG CATCTCCTGC 
ATCCTCGTGA AAACCAACAG GGTTCTTTTG GTGTTTGAAG CCAAGATCCC CACCAGCATC 
CATCGTAAAT GGTGGGGCCT GAACCTGCAG TTCCTCCTGG TGTTTCTGTG CACCTTCGTT 
CAGGTCATGA TATGTGTGGT CTGGCTTTAC AACGCCCCCC CCTCCAGCTA CAGGAATCAT 
GACATCGATG AGATCATTTT CATCACCTGT AATGAGGGAT CCGTGATGGC GCTCGGGTTT 
CTCATTGGCT ACACGTGCTT ACTAGCGGCC ATATGCTTCT TCTTTGCGTT TAAGTCAAGG 
AAACTTCCCG AAAACTTCAC GGAGGCCAAG TTCATCACTT TCTGCATGCT CATCTTCTTT 
ATTGTTTGGA TTTCTTTTAT CCCTGCGTAC TTCAGTACTT ACGGCAAGTT TGTTTCAGCA 
GTAGAGGCCA TTGCCATCCT GGCATCCAGC TACGGGATGC TAGCTTGTAT TTTCTTTAAT 
AAGGTCTACA TCATCCTGTT CAAACCCTGT AGGAACACCA TAGAAGAGGT CAGGTGCAGC 
ACCGCGGCCC ACGCTTTCAG AGTGGCCGCC AAAGCTACAT TAAAACACAG AACCACTGTG 
AGGAAAAAGT CGAACAGCAT CGGCTCTACC GCCTCCACTC CATCATCATC AATTAGCCTG 
AAGACCAACA GCAACGACTG CGACTCCGCC TCAGGGAGGC ATAGGCCGAG GGTGAGCTTC 
GGCAGCGGAA CTGTCATGCT GTCCTTGAGC TTTGAGGAGT CCAGGAGGAG CTCTCTGATG 
TGA