LOC101078293 cDNA ORF clone, Takifugu rubripes(torafugu)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC101078293
Entrez Gene ID	101078293
Full Name	alsin-like
Gene Type	protein-coding
Organism	Takifugu rubripes(torafugu)

mRNA and Protein(s)

mRNA	Protein	Name
XM_011611395.2	XP_011609697.2	alsin-like isoform X1
XM_011611399.2	XP_011609701.2	alsin-like isoform X2
XM_029843632.1	XP_029699492.1	alsin-like isoform X4
XM_011611399.1	XP_011609701.1	alsin-like isoform X2
XM_011611395.1	XP_011609697.1	alsin-like isoform X1
XM_011611404.1	XP_011609706.1	alsin-like isoform X3
XM_011611411.1	XP_011609713.1	alsin-like isoform X3
XM_011611411.2	XP_011609713.2	alsin-like isoform X3

Pathways from BioSystems
Gene Ontology

The following LOC101078293 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC101078293 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OTo42294	XM_011611411.2 Latest version!	Takifugu rubripes alsin-like (LOC101078293), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OTo42295	XM_029843632.1 Latest version!	Takifugu rubripes alsin-like (LOC101078293), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OTo42292	XM_011611395.2 Latest version!	Takifugu rubripes alsin-like (LOC101078293), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OTo42293	XM_011611399.2 Latest version!	Takifugu rubripes alsin-like (LOC101078293), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OTo30993	XM_011611395.1 Latest version!	Takifugu rubripes alsin-like (LOC101078293), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OTo30994	XM_011611399.1 Latest version!	Takifugu rubripes alsin-like (LOC101078293), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OTo30995	XM_011611404.1 Latest version!	Takifugu rubripes alsin-like (LOC101078293), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OTo30995	XM_011611411.1 Latest version!	Takifugu rubripes alsin-like (LOC101078293), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC101078293 ( XM_011611411.2 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_011609713.2 Takifugu rubripes alsin-like (LOC101078293), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OTo42294
Clone ID Related Accession (Same CDS sequence)	XM_011611411.2
Accession Version	XM_011611411.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4068bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-04
Organism	Takifugu rubripes(torafugu)
Product	alsin-like isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042285.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jul 5, 2019 this sequence version replaced XM_011611411.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTATTCA GCTTGGCCCT GGTTCGTAGT TTGCCTCCAA ATAGCTACAA CAGCCAGAAG 
CCTTCTGAAA AAAGGGAACG GGGTCACTCG CCGCACTATT CAGCAGCAGA GAGAGAGGAG 
CTGTTTGTGG GTGACAGCAG TCTCTACTGT CCACTTGGGG TGGAGCTGAA AGAAGGCATG 
GCCACAGAGA CTTCCTCCAG GAGATGTCCA AGGAGAAGGC TCCATCCTGG AGGAAGTTCG 
GCCAGCAGCA GCCCTGGCTC TGCATTTAAC GAAGACGCGA CCAAAGACAC TGACTCACTG 
AGCAGCTGCA CCTCAGATGA CAGCTGTGTT TCCTCAACTC TCTCCACTGA TATGCTGACC 
TCCAGTTACA AAGAACCTAA CAATAACTTT GGAGTGACCC TGCCGTGCTC GTCCTCCTCA 
CTGTGTCTCG ACGATGTCCG ACTCTGTCTT GAATCGGGGC TGCAGGTACA GCCAGGGAAG 
AAGCTCTCCA GCCTCTCCAA TAAACACTAT AAAGCAAAAT CAGCAGGAAC CAGAAGACAT 
TCTGTCCCTG GGACACCCAC TCGTGGGTCT CCACGGCGAC AGCGTGCAAA CGCGTGCGGA 
AAATCTTCTG GTGGTCAGAC CCAGGCGGGA GCGCCGGAGG AGGATGATGA TGATGGGCTG 
CCATCTCTAG ACACAGAAGT CTGGAGCTGG GGACGGGGCT CTGAGGGGCA GCTCGGCCAC 
GGAGATCAGC TCGCCAGACT TCAGCCTCTG TGTATCAAGA CACTGTCGGG TGAGGAGGTG 
ATCCAAGTTG CAGCAGGGTC CCATCATTCT CTGGCTGTCA CTGCAACCTG TCAGGTGTAC 
TCATGGGGCA GCAACATGTG TGGGCAGCTG GGTCACGATA ACAGTTCTTT CGTTGTGCCC 
CAACAGGCAA AGCTGTCTGA TGGCCTCCGA GTTTGGGACA TGTCTGCCGG CCAGAGCCAC 
TCCCTCCTCC TGGCTGATGG AGACTGCGTC CAGCCCGTGT TGTTGTACTG TGGCCGGCAG 
AAAGAGTCGA CATCAGACCA GAGAGAAAAG TCCTACCAGA GGTCCCCCAG AAAAGCAGAG 
CATTACACAG TCAGGCCCAC CCTGCTGCCT GTCTGCCTGG AGATGGGCTA CATCAGCAGT 
GTGTGCAGCG GCGGGCAAAG CTGCGCCGTC CTGGCAGACC AGAACATCAA GGTTTTTATT 
TCGGCCCTCC ACGAGCTGGC CTCCAGAGAG AGGCAGTTCT ACTGCTGGTT GAGTCGCGTC 
AGGAAACTCG TCCTCACACC AATACAGAAC AAAGAGAGTA CGTCTCCGTT TTTAGGTGAC 
CCTTGCACCC ATCTCTTTTC TTCTCTCTGT GGGAGTTTTT CTCAGCTAAG CGTCCTAATC 
GGTCGACACT CCACATCGCT TACCTACTTC CTGCAAAAGG CACAGGGCCG TGACGTCACC 
TCACTCCCCC TGCTCACACA CATGGAGCAG TTTCTGGACA CTTACAAAGA ATATTGTTCC 
TCTGTTGGTG ACTTTCAGGT GATGGGGGGC TTCCAGACCC TCCATAAGCT CACACTTGAG 
TGTTTGCAGC CAGAAGTTCT CACTCAGCTG TGTATGTCGG ACCAGACCAG TGATGACACT 
GGTCTGGTTT CGCTGTTGTA CTGGCCTCTG CAGCAGCTCC ACCAGTACGA CCGAGTCATG 
GTCAAAGTGG CATTTTGCTT CGACGTGTTA ATGCCAGAGT TTCAGTCCTT CCGTCAGGGC 
TGCGGTCAGT ACGAGTCCCT GTCTCTGTCC CTGTTGAGGA AGAAAAAAGA GGCTGAAAGC 
ACATTCCTCT TCTGGAAGAG CCACTCCGGA AAAACTACTG AGAGTCTACG TCTCCCTCGG 
CGCCGAGTGG TGTGTGAAAG CAGCAGCAGA TCTTTGACTC TGCAAAAGGC CGGGCGCTTC 
TCCAACCACT GGTTCATTCT GTTCAATGAT GCACTGGTCC ACACGCAGGG CGCGCTTCCC 
TCACAGCTCT TTTTCTCCAC ACATCACGTC TACCCCCTGA CCTGCCTGTG GGTGAAACCC 
GTCACTGACG ACAGCAGCGG GCTCTATGCC ATTAAAATTA TTTGCCCGGA GGAAAGTTTC 
ACCATGGTGG CTTCAACACC TCAAGAAAAG AACAAGTGGT TACGATCTAT CAATCACGCG 
CTGGATCAGG TGCTCGGTGG TAGTGGTGAG GGGTCGTCGC CGGGGACGAT GGGAATGTCC 
CGCACAGCCT CCTACACATT CACCGGAGAA GGCCGGTTTA AAGATGCCAA ATACAGCGGC 
AGCTGGATGT CAGGACGGGT GCAGGGCAGA GGGTCCTTGA GGTGGCCTGA TGGGCGGACC 
TACTGCGGGA ACTTCAAGAA CGGCCTTGAA GATGGTTACG GGGAGTGCGT AATACCCAAC 
AAAGAGCTGA ACAAGCCTGA CAGTTACCAT GGACACTGGA GAGAGGGGAA GATCCACGGT 
TTCGGCAAAT ACAAGTACGC CAGCGGCGAG GTGTATGAAG GCTGTTTCAG TGAAGGGCAG 
CGGCACGGTT ACGGCATGCT GAGCTCCGGG AAGCTGGGGA AGAAGTCCTC CAGTGTTTTC 
ATCGGCCACT GGGTCCATGA CAAGAAGACA GGTTATGGAG TGTTTGACGA CATCACCAGG 
GGTGAGAAGT ACATGGGATT GTGGGTAGAC GAGAAGCGGC ACGGCAGCGG CGTGGTGGTC 
ACACAGTATG GCGTGTACTA CGAAGGAACC TTCAGAGACA ACAAGATGAG TGGTCCCGGT 
CTGCTGATGT CGGATGACGA CACGGCTATT CATGGGGAGT TTTCCGACGA TTGGGTTGTC 
AACGGGAAGG GTGTTTTATT GTTGGCCAAC GGAGACTGTC TGGAGGGTCT GTTCAGTGGG 
GAGTGGACAT CTGGACTGAA GGTGGATGGA ACGTACACCA AACCACTGGT GGACGAGCCT 
GTAAACAAAG AAAGAAAAAA CATGTTCAAG CTGGGTAGGT ACGTGGTGCC TGCGGGTCAG 
AGGTGGGTCT GTGTGTTTGA GGAGTGCTGG AGTCGACTGG GCTGCGACAG CACTGGGAGA 
GGAGACAAAA ATGTAGCCTG GGAGAACATC GCAGTGTCCA TAACGGCTGC ACGGAGGCAA 
AGTCCAGATC TCAGTCGGTC TCAGAACAAA GTGTTGGAGT CTCTGGAGTT TATCCCTCAG 
CATGAGCAGC CAGTCACCAC CTCGAACTAC GACAGCATAC GCAGATACCT CATCAAGGCT 
TGTGAGACCC CCCTCCACCC TCTGGGCTGG CTGGTGGAGA CTCTGGTGAC GGTGTACCGG 
ATGACGTATG TGGGCGTGGA AGCTAACCGG AGGCTGCTGG AACAGGCTGT CCAGGAGCTT 
CAGGCCTACC TCACACATCT CTACAACATT GTCCAGTTTT TGTTTCCAGG GATGCCAGAT 
GATGGCGCCG TCATCCCAGA CCCCCCGGGG TCTCCATCGG TAGTCCAGAA CAGCTCCAGC 
ATGGAAGACT TGAGTGCTGT AGTGGTGAGC TGCTCCTCTC TGCTCCTCCC TCTGCTCCTG 
CCTCGACTCT ACCCTCCTCT CTTCACGCTG TACCGGCTGC AGGAGGAGCA GGAAGACGCA 
CTGTACTGGG ACCGCATCCT GCGTCTGAAC AAACAACCCG ATCAGTCGCT GCTGAGCTTC 
CTGGGAGTGC AAGAGAAGTT CTGGCCTACG TGGATGTCAA TTTTGGGGGA GAAGAAGCAG 
ATTGTGTCGA GCAGCAAAGA CGCCTGCTTC GTTTCCGCAG TTGAGACCCT CCAACAAATC 
AGCACCACCT TTACTCCATC TGACAAACTC GTCGTCATCC AGAAGACCTT TGAGGAGTTG 
ACCCAGGAAG TCAATCCCAT GCTGGACGGC CACTTCCTGT GGTGCATGGA TGACTTGCTC 
CCACTCTTCA TCTACGTGGT GGTCAGAGCG AGGATCAGGA ATCTGGGAGC GGAGGTCGAT 
ATGATTGAAG ATTTGATGGA TCCGAATGTT CAGCATGGAG AGCTGGGACT GATGTTCACC 
ACCCTGAAGG CCTGCTACAT CCAGATCAAA AACGAGTCTA CGACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011609713.2
CDS	139..4206
Translation	MLFSLALVRSLPPNSYNSQKPSEKRERGHSPHYSAAEREELFVGDSSLYCPLGVELKEGMATETSSRRCPRRRLHPGGSSASSSPGSAFNEDATKDTDSLSSCTSDDSCVSSTLSTDMLTSSYKEPNNNFGVTLPCSSSSLCLDDVRLCLESGLQVQPGKKLSSLSNKHYKAKSAGTRRHSVPGTPTRGSPRRQRANACGKSSGGQTQAGAPEEDDDDGLPSLDTEVWSWGRGSEGQLGHGDQLARLQPLCIKTLSGEEVIQVAAGSHHSLAVTATCQVYSWGSNMCGQLGHDNSSFVVPQQAKLSDGLRVWDMSAGQSHSLLLADGDCVQPVLLYCGRQKESTSDQREKSYQRSPRKAEHYTVRPTLLPVCLEMGYISSVCSGGQSCAVLADQNIKVFISALHELASRERQFYCWLSRVRKLVLTPIQNKESTSPFLGDPCTHLFSSLCGSFSQLSVLIGRHSTSLTYFLQKAQGRDVTSLPLLTHMEQFLDTYKEYCSSVGDFQVMGGFQTLHKLTLECLQPEVLTQLCMSDQTSDDTGLVSLLYWPLQQLHQYDRVMVKVAFCFDVLMPEFQSFRQGCGQYESLSLSLLRKKKEAESTFLFWKSHSGKTTESLRLPRRRVVCESSSRSLTLQKAGRFSNHWFILFNDALVHTQGALPSQLFFSTHHVYPLTCLWVKPVTDDSSGLYAIKIICPEESFTMVASTPQEKNKWLRSINHALDQVLGGSGEGSSPGTMGMSRTASYTFTGEGRFKDAKYSGSWMSGRVQGRGSLRWPDGRTYCGNFKNGLEDGYGECVIPNKELNKPDSYHGHWREGKIHGFGKYKYASGEVYEGCFSEGQRHGYGMLSSGKLGKKSSSVFIGHWVHDKKTGYGVFDDITRGEKYMGLWVDEKRHGSGVVVTQYGVYYEGTFRDNKMSGPGLLMSDDDTAIHGEFSDDWVVNGKGVLLLANGDCLEGLFSGEWTSGLKVDGTYTKPLVDEPVNKERKNMFKLGRYVVPAGQRWVCVFEECWSRLGCDSTGRGDKNVAWENIAVSITAARRQSPDLSRSQNKVLESLEFIPQHEQPVTTSNYDSIRRYLIKACETPLHPLGWLVETLVTVYRMTYVGVEANRRLLEQAVQELQAYLTHLYNIVQFLFPGMPDDGAVIPDPPGSPSVVQNSSSMEDLSAVVVSCSSLLLPLLLPRLYPPLFTLYRLQEEQEDALYWDRILRLNKQPDQSLLSFLGVQEKFWPTWMSILGEKKQIVSSSKDACFVSAVETLQQISTTFTPSDKLVVIQKTFEELTQEVNPMLDGHFLWCMDDLLPLFIYVVVRARIRNLGAEVDMIEDLMDPNVQHGELGLMFTTLKACYIQIKNESTT

Target ORF information:

RefSeq Version	XM_011611411.2
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes alsin-like (LOC101078293), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011611411.2

ORF Insert Sequence:

ATGTTATTCA GCTTGGCCCT GGTTCGTAGT TTGCCTCCAA ATAGCTACAA CAGCCAGAAG 
CCTTCTGAAA AAAGGGAACG GGGTCACTCG CCGCACTATT CAGCAGCAGA GAGAGAGGAG 
CTGTTTGTGG GTGACAGCAG TCTCTACTGT CCACTTGGGG TGGAGCTGAA AGAAGGCATG 
GCCACAGAGA CTTCCTCCAG GAGATGTCCA AGGAGAAGGC TCCATCCTGG AGGAAGTTCG 
GCCAGCAGCA GCCCTGGCTC TGCATTTAAC GAAGACGCGA CCAAAGACAC TGACTCACTG 
AGCAGCTGCA CCTCAGATGA CAGCTGTGTT TCCTCAACTC TCTCCACTGA TATGCTGACC 
TCCAGTTACA AAGAACCTAA CAATAACTTT GGAGTGACCC TGCCGTGCTC GTCCTCCTCA 
CTGTGTCTCG ACGATGTCCG ACTCTGTCTT GAATCGGGGC TGCAGGTACA GCCAGGGAAG 
AAGCTCTCCA GCCTCTCCAA TAAACACTAT AAAGCAAAAT CAGCAGGAAC CAGAAGACAT 
TCTGTCCCTG GGACACCCAC TCGTGGGTCT CCACGGCGAC AGCGTGCAAA CGCGTGCGGA 
AAATCTTCTG GTGGTCAGAC CCAGGCGGGA GCGCCGGAGG AGGATGATGA TGATGGGCTG 
CCATCTCTAG ACACAGAAGT CTGGAGCTGG GGACGGGGCT CTGAGGGGCA GCTCGGCCAC 
GGAGATCAGC TCGCCAGACT TCAGCCTCTG TGTATCAAGA CACTGTCGGG TGAGGAGGTG 
ATCCAAGTTG CAGCAGGGTC CCATCATTCT CTGGCTGTCA CTGCAACCTG TCAGGTGTAC 
TCATGGGGCA GCAACATGTG TGGGCAGCTG GGTCACGATA ACAGTTCTTT CGTTGTGCCC 
CAACAGGCAA AGCTGTCTGA TGGCCTCCGA GTTTGGGACA TGTCTGCCGG CCAGAGCCAC 
TCCCTCCTCC TGGCTGATGG AGACTGCGTC CAGCCCGTGT TGTTGTACTG TGGCCGGCAG 
AAAGAGTCGA CATCAGACCA GAGAGAAAAG TCCTACCAGA GGTCCCCCAG AAAAGCAGAG 
CATTACACAG TCAGGCCCAC CCTGCTGCCT GTCTGCCTGG AGATGGGCTA CATCAGCAGT 
GTGTGCAGCG GCGGGCAAAG CTGCGCCGTC CTGGCAGACC AGAACATCAA GGTTTTTATT 
TCGGCCCTCC ACGAGCTGGC CTCCAGAGAG AGGCAGTTCT ACTGCTGGTT GAGTCGCGTC 
AGGAAACTCG TCCTCACACC AATACAGAAC AAAGAGAGTA CGTCTCCGTT TTTAGGTGAC 
CCTTGCACCC ATCTCTTTTC TTCTCTCTGT GGGAGTTTTT CTCAGCTAAG CGTCCTAATC 
GGTCGACACT CCACATCGCT TACCTACTTC CTGCAAAAGG CACAGGGCCG TGACGTCACC 
TCACTCCCCC TGCTCACACA CATGGAGCAG TTTCTGGACA CTTACAAAGA ATATTGTTCC 
TCTGTTGGTG ACTTTCAGGT GATGGGGGGC TTCCAGACCC TCCATAAGCT CACACTTGAG 
TGTTTGCAGC CAGAAGTTCT CACTCAGCTG TGTATGTCGG ACCAGACCAG TGATGACACT 
GGTCTGGTTT CGCTGTTGTA CTGGCCTCTG CAGCAGCTCC ACCAGTACGA CCGAGTCATG 
GTCAAAGTGG CATTTTGCTT CGACGTGTTA ATGCCAGAGT TTCAGTCCTT CCGTCAGGGC 
TGCGGTCAGT ACGAGTCCCT GTCTCTGTCC CTGTTGAGGA AGAAAAAAGA GGCTGAAAGC 
ACATTCCTCT TCTGGAAGAG CCACTCCGGA AAAACTACTG AGAGTCTACG TCTCCCTCGG 
CGCCGAGTGG TGTGTGAAAG CAGCAGCAGA TCTTTGACTC TGCAAAAGGC CGGGCGCTTC 
TCCAACCACT GGTTCATTCT GTTCAATGAT GCACTGGTCC ACACGCAGGG CGCGCTTCCC 
TCACAGCTCT TTTTCTCCAC ACATCACGTC TACCCCCTGA CCTGCCTGTG GGTGAAACCC 
GTCACTGACG ACAGCAGCGG GCTCTATGCC ATTAAAATTA TTTGCCCGGA GGAAAGTTTC 
ACCATGGTGG CTTCAACACC TCAAGAAAAG AACAAGTGGT TACGATCTAT CAATCACGCG 
CTGGATCAGG TGCTCGGTGG TAGTGGTGAG GGGTCGTCGC CGGGGACGAT GGGAATGTCC 
CGCACAGCCT CCTACACATT CACCGGAGAA GGCCGGTTTA AAGATGCCAA ATACAGCGGC 
AGCTGGATGT CAGGACGGGT GCAGGGCAGA GGGTCCTTGA GGTGGCCTGA TGGGCGGACC 
TACTGCGGGA ACTTCAAGAA CGGCCTTGAA GATGGTTACG GGGAGTGCGT AATACCCAAC 
AAAGAGCTGA ACAAGCCTGA CAGTTACCAT GGACACTGGA GAGAGGGGAA GATCCACGGT 
TTCGGCAAAT ACAAGTACGC CAGCGGCGAG GTGTATGAAG GCTGTTTCAG TGAAGGGCAG 
CGGCACGGTT ACGGCATGCT GAGCTCCGGG AAGCTGGGGA AGAAGTCCTC CAGTGTTTTC 
ATCGGCCACT GGGTCCATGA CAAGAAGACA GGTTATGGAG TGTTTGACGA CATCACCAGG 
GGTGAGAAGT ACATGGGATT GTGGGTAGAC GAGAAGCGGC ACGGCAGCGG CGTGGTGGTC 
ACACAGTATG GCGTGTACTA CGAAGGAACC TTCAGAGACA ACAAGATGAG TGGTCCCGGT 
CTGCTGATGT CGGATGACGA CACGGCTATT CATGGGGAGT TTTCCGACGA TTGGGTTGTC 
AACGGGAAGG GTGTTTTATT GTTGGCCAAC GGAGACTGTC TGGAGGGTCT GTTCAGTGGG 
GAGTGGACAT CTGGACTGAA GGTGGATGGA ACGTACACCA AACCACTGGT GGACGAGCCT 
GTAAACAAAG AAAGAAAAAA CATGTTCAAG CTGGGTAGGT ACGTGGTGCC TGCGGGTCAG 
AGGTGGGTCT GTGTGTTTGA GGAGTGCTGG AGTCGACTGG GCTGCGACAG CACTGGGAGA 
GGAGACAAAA ATGTAGCCTG GGAGAACATC GCAGTGTCCA TAACGGCTGC ACGGAGGCAA 
AGTCCAGATC TCAGTCGGTC TCAGAACAAA GTGTTGGAGT CTCTGGAGTT TATCCCTCAG 
CATGAGCAGC CAGTCACCAC CTCGAACTAC GACAGCATAC GCAGATACCT CATCAAGGCT 
TGTGAGACCC CCCTCCACCC TCTGGGCTGG CTGGTGGAGA CTCTGGTGAC GGTGTACCGG 
ATGACGTATG TGGGCGTGGA AGCTAACCGG AGGCTGCTGG AACAGGCTGT CCAGGAGCTT 
CAGGCCTACC TCACACATCT CTACAACATT GTCCAGTTTT TGTTTCCAGG GATGCCAGAT 
GATGGCGCCG TCATCCCAGA CCCCCCGGGG TCTCCATCGG TAGTCCAGAA CAGCTCCAGC 
ATGGAAGACT TGAGTGCTGT AGTGGTGAGC TGCTCCTCTC TGCTCCTCCC TCTGCTCCTG 
CCTCGACTCT ACCCTCCTCT CTTCACGCTG TACCGGCTGC AGGAGGAGCA GGAAGACGCA 
CTGTACTGGG ACCGCATCCT GCGTCTGAAC AAACAACCCG ATCAGTCGCT GCTGAGCTTC 
CTGGGAGTGC AAGAGAAGTT CTGGCCTACG TGGATGTCAA TTTTGGGGGA GAAGAAGCAG 
ATTGTGTCGA GCAGCAAAGA CGCCTGCTTC GTTTCCGCAG TTGAGACCCT CCAACAAATC 
AGCACCACCT TTACTCCATC TGACAAACTC GTCGTCATCC AGAAGACCTT TGAGGAGTTG 
ACCCAGGAAG TCAATCCCAT GCTGGACGGC CACTTCCTGT GGTGCATGGA TGACTTGCTC 
CCACTCTTCA TCTACGTGGT GGTCAGAGCG AGGATCAGGA ATCTGGGAGC GGAGGTCGAT 
ATGATTGAAG ATTTGATGGA TCCGAATGTT CAGCATGGAG AGCTGGGACT GATGTTCACC 
ACCCTGAAGG CCTGCTACAT CCAGATCAAA AACGAGTCTA CGACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101078293 ( XM_029843632.1 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_029699492.1 Takifugu rubripes alsin-like (LOC101078293), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OTo42295
Clone ID Related Accession (Same CDS sequence)	XM_029843632.1
Accession Version	XM_029843632.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3891bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-04
Organism	Takifugu rubripes(torafugu)
Product	alsin-like isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042285.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACAG AGACTTCCTC CAGGAGATGT CCAAGGAGAA GGCTCCATCC TGGAGGAAGT 
TCGGCCAGCA GCAGCCCTGG CTCTGCATTT AACGAAGACG CGACCAAAGA CACTGACTCA 
CTGAGCAGCT GCACCTCAGA TGACAGCTGT GTTTCCTCAA CTCTCTCCAC TGATATGCTG 
ACCTCCAGTT ACAAAGAACC TAACAATAAC TTTGGAGTGA CCCTGCCGTG CTCGTCCTCC 
TCACTGTGTC TCGACGATGT CCGACTCTGT CTTGAATCGG GGCTGCAGGT ACAGCCAGGG 
AAGAAGCTCT CCAGCCTCTC CAATAAACAC TATAAAGCAA AATCAGCAGG AACCAGAAGA 
CATTCTGTCC CTGGGACACC CACTCGTGGG TCTCCACGGC GACAGCGTGC AAACGCGTGC 
GGAAAATCTT CTGGTGGTCA GACCCAGGCG GGAGCGCCGG AGGAGGATGA TGATGATGGG 
CTGCCATCTC TAGACACAGA AGTCTGGAGC TGGGGACGGG GCTCTGAGGG GCAGCTCGGC 
CACGGAGATC AGCTCGCCAG ACTTCAGCCT CTGTGTATCA AGACACTGTC GGGTGAGGAG 
GTGATCCAAG TTGCAGCAGG GTCCCATCAT TCTCTGGCTG TCACTGCAAC CTGTCAGGTG 
TACTCATGGG GCAGCAACAT GTGTGGGCAG CTGGGTCACG ATAACAGTTC TTTCGTTGTG 
CCCCAACAGG CAAAGCTGTC TGATGGCCTC CGAGTTTGGG ACATGTCTGC CGGCCAGAGC 
CACTCCCTCC TCCTGGCTGA TGGAGACTGC GTCCAGCCCG TGTTGTTGTA CTGTGGCCGG 
CAGAAAGAGT CGACATCAGA CCAGAGAGAA AAGTCCTACC AGAGGTCCCC CAGAAAAGCA 
GAGCATTACA CAGTCAGGCC CACCCTGCTG CCTGTCTGCC TGGAGATGGG CTACATCAGC 
AGTGTGTGCA GCGGCGGGCA AAGCTGCGCC GTCCTGGCAG ACCAGAACAT CAAGGTTTTT 
ATTTCGGCCC TCCACGAGCT GGCCTCCAGA GAGAGGCAGT TCTACTGCTG GTTGAGTCGC 
GTCAGGAAAC TCGTCCTCAC ACCAATACAG AACAAAGAGA GTACGTCTCC GTTTTTAGGT 
GACCCTTGCA CCCATCTCTT TTCTTCTCTC TGTGGGAGTT TTTCTCAGCT AAGCGTCCTA 
ATCGGTCGAC ACTCCACATC GCTTACCTAC TTCCTGCAAA AGGCACAGGG CCGTGACGTC 
ACCTCACTCC CCCTGCTCAC ACACATGGAG CAGTTTCTGG ACACTTACAA AGAATATTGT 
TCCTCTGTTG GTGACTTTCA GGTGATGGGG GGCTTCCAGA CCCTCCATAA GCTCACACTT 
GAGTGTTTGC AGCCAGAAGT TCTCACTCAG CTGTGTATGT CGGACCAGAC CAGTGATGAC 
ACTGGTCTGG TTTCGCTGTT GTACTGGCCT CTGCAGCAGC TCCACCAGTA CGACCGAGTC 
ATGGTCAAAG TGGCATTTTG CTTCGACGTG TTAATGCCAG AGTTTCAGTC CTTCCGTCAG 
GGCTGCGGTC AGTACGAGTC CCTGTCTCTG TCCCTGTTGA GGAAGAAAAA AGAGGCTGAA 
AGCACATTCC TCTTCTGGAA GAGCCACTCC GGAAAAACTA CTGAGAGTCT ACGTCTCCCT 
CGGCGCCGAG TGGTGTGTGA AAGCAGCAGC AGATCTTTGA CTCTGCAAAA GGCCGGGCGC 
TTCTCCAACC ACTGGTTCAT TCTGTTCAAT GATGCACTGG TCCACACGCA GGGCGCGCTT 
CCCTCACAGC TCTTTTTCTC CACACATCAC GTCTACCCCC TGACCTGCCT GTGGGTGAAA 
CCCGTCACTG ACGACAGCAG CGGGCTCTAT GCCATTAAAA TTATTTGCCC GGAGGAAAGT 
TTCACCATGG TGGCTTCAAC ACCTCAAGAA AAGAACAAGT GGTTACGATC TATCAATCAC 
GCGCTGGATC AGGTGCTCGG TGGTAGTGGT GAGGGGTCGT CGCCGGGGAC GATGGGAATG 
TCCCGCACAG CCTCCTACAC ATTCACCGGA GAAGGCCGGT TTAAAGATGC CAAATACAGC 
GGCAGCTGGA TGTCAGGACG GGTGCAGGGC AGAGGGTCCT TGAGGTGGCC TGATGGGCGG 
ACCTACTGCG GGAACTTCAA GAACGGCCTT GAAGATGGTT ACGGGGAGTG CGTAATACCC 
AACAAAGAGC TGAACAAGCC TGACAGTTAC CATGGACACT GGAGAGAGGG GAAGATCCAC 
GGTTTCGGCA AATACAAGTA CGCCAGCGGC GAGGTGTATG AAGGCTGTTT CAGTGAAGGG 
CAGCGGCACG GTTACGGCAT GCTGAGCTCC GGGAAGCTGG GGAAGAAGTC CTCCAGTGTT 
TTCATCGGCC ACTGGGTCCA TGACAAGAAG ACAGGTTATG GAGTGTTTGA CGACATCACC 
AGGGGTGAGA AGTACATGGG ATTGTGGGTA GACGAGAAGC GGCACGGCAG CGGCGTGGTG 
GTCACACAGT ATGGCGTGTA CTACGAAGGA ACCTTCAGAG ACAACAAGAT GAGTGGTCCC 
GGTCTGCTGA TGTCGGATGA CGACACGGCT ATTCATGGGG AGTTTTCCGA CGATTGGGTT 
GTCAACGGGA AGGGTGTTTT ATTGTTGGCC AACGGAGACT GTCTGGAGGG TCTGTTCAGT 
GGGGAGTGGA CATCTGGACT GAAGGTGGAT GGAACGTACA CCAAACCACT GGTGGACGAG 
CCTGTAAACA AAGAAAGAAA AAACATGTTC AAGCTGGGTA GGTACGTGGT GCCTGCGGGT 
CAGAGGTGGG TCTGTGTGTT TGAGGAGTGC TGGAGTCGAC TGGGCTGCGA CAGCACTGGG 
AGAGGAGACA AAAATGTAGC CTGGGAGAAC ATCGCAGTGT CCATAACGGC TGCACGGAGG 
CAAAGTCCAG ATCTCAGTCG GTCTCAGAAC AAAGTGTTGG AGTCTCTGGA GTTTATCCCT 
CAGCATGAGC AGCCAGTCAC CACCTCGAAC TACGACAGCA TACGCAGATA CCTCATCAAG 
GCTTGTGAGA CCCCCCTCCA CCCTCTGGGC TGGCTGGTGG AGACTCTGGT GACGGTGTAC 
CGGATGACGT ATGTGGGCGT GGAAGCTAAC CGGAGGCTGC TGGAACAGGC TGTCCAGGAG 
CTTCAGGCCT ACCTCACACA TCTCTACAAC ATTGTCCAGT TTTTGTTTCC AGGGATGCCA 
GATGATGGCG CCGTCATCCC AGACCCCCCG GGGTCTCCAT CGGTAGTCCA GAACAGCTCC 
AGCATGGAAG ACTTGAGTGC TGTAGTGGTG AGCTGCTCCT CTCTGCTCCT CCCTCTGCTC 
CTGCCTCGAC TCTACCCTCC TCTCTTCACG CTGTACCGGC TGCAGGAGGA GCAGGAAGAC 
GCACTGTACT GGGACCGCAT CCTGCGTCTG AACAAACAAC CCGATCAGTC GCTGCTGAGC 
TTCCTGGGAG TGCAAGAGAA GTTCTGGCCT ACGTGGATGT CAATTTTGGG GGAGAAGAAG 
CAGATTGTGT CGAGCAGCAA AGACGCCTGC TTCGTTTCCG CAGTTGAGAC CCTCCAACAA 
ATCAGCACCA CCTTTACTCC ATCTGACAAA CTCGTCGTCA TCCAGAAGAC CTTTGAGGAG 
TTGACCCAGG AAGTCAATCC CATGCTGGAC GGCCACTTCC TGTGGTGCAT GGATGACTTG 
CTCCCACTCT TCATCTACGT GGTGGTCAGA GCGAGGATCA GGAATCTGGG AGCGGAGGTC 
GATATGATTG AAGATTTGAT GGATCCGAAT GTTCAGCATG GAGAGCTGGG ACTGATGTTC 
ACCACCCTGA AGGCCTGCTA CATCCAGATC AAAAACGAGT CTACGACATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029699492.1
CDS	344..4234
Translation	MATETSSRRCPRRRLHPGGSSASSSPGSAFNEDATKDTDSLSSCTSDDSCVSSTLSTDMLTSSYKEPNNNFGVTLPCSSSSLCLDDVRLCLESGLQVQPGKKLSSLSNKHYKAKSAGTRRHSVPGTPTRGSPRRQRANACGKSSGGQTQAGAPEEDDDDGLPSLDTEVWSWGRGSEGQLGHGDQLARLQPLCIKTLSGEEVIQVAAGSHHSLAVTATCQVYSWGSNMCGQLGHDNSSFVVPQQAKLSDGLRVWDMSAGQSHSLLLADGDCVQPVLLYCGRQKESTSDQREKSYQRSPRKAEHYTVRPTLLPVCLEMGYISSVCSGGQSCAVLADQNIKVFISALHELASRERQFYCWLSRVRKLVLTPIQNKESTSPFLGDPCTHLFSSLCGSFSQLSVLIGRHSTSLTYFLQKAQGRDVTSLPLLTHMEQFLDTYKEYCSSVGDFQVMGGFQTLHKLTLECLQPEVLTQLCMSDQTSDDTGLVSLLYWPLQQLHQYDRVMVKVAFCFDVLMPEFQSFRQGCGQYESLSLSLLRKKKEAESTFLFWKSHSGKTTESLRLPRRRVVCESSSRSLTLQKAGRFSNHWFILFNDALVHTQGALPSQLFFSTHHVYPLTCLWVKPVTDDSSGLYAIKIICPEESFTMVASTPQEKNKWLRSINHALDQVLGGSGEGSSPGTMGMSRTASYTFTGEGRFKDAKYSGSWMSGRVQGRGSLRWPDGRTYCGNFKNGLEDGYGECVIPNKELNKPDSYHGHWREGKIHGFGKYKYASGEVYEGCFSEGQRHGYGMLSSGKLGKKSSSVFIGHWVHDKKTGYGVFDDITRGEKYMGLWVDEKRHGSGVVVTQYGVYYEGTFRDNKMSGPGLLMSDDDTAIHGEFSDDWVVNGKGVLLLANGDCLEGLFSGEWTSGLKVDGTYTKPLVDEPVNKERKNMFKLGRYVVPAGQRWVCVFEECWSRLGCDSTGRGDKNVAWENIAVSITAARRQSPDLSRSQNKVLESLEFIPQHEQPVTTSNYDSIRRYLIKACETPLHPLGWLVETLVTVYRMTYVGVEANRRLLEQAVQELQAYLTHLYNIVQFLFPGMPDDGAVIPDPPGSPSVVQNSSSMEDLSAVVVSCSSLLLPLLLPRLYPPLFTLYRLQEEQEDALYWDRILRLNKQPDQSLLSFLGVQEKFWPTWMSILGEKKQIVSSSKDACFVSAVETLQQISTTFTPSDKLVVIQKTFEELTQEVNPMLDGHFLWCMDDLLPLFIYVVVRARIRNLGAEVDMIEDLMDPNVQHGELGLMFTTLKACYIQIKNESTT

Target ORF information:

RefSeq Version	XM_029843632.1
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes alsin-like (LOC101078293), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029843632.1

ORF Insert Sequence:

ATGGCCACAG AGACTTCCTC CAGGAGATGT CCAAGGAGAA GGCTCCATCC TGGAGGAAGT 
TCGGCCAGCA GCAGCCCTGG CTCTGCATTT AACGAAGACG CGACCAAAGA CACTGACTCA 
CTGAGCAGCT GCACCTCAGA TGACAGCTGT GTTTCCTCAA CTCTCTCCAC TGATATGCTG 
ACCTCCAGTT ACAAAGAACC TAACAATAAC TTTGGAGTGA CCCTGCCGTG CTCGTCCTCC 
TCACTGTGTC TCGACGATGT CCGACTCTGT CTTGAATCGG GGCTGCAGGT ACAGCCAGGG 
AAGAAGCTCT CCAGCCTCTC CAATAAACAC TATAAAGCAA AATCAGCAGG AACCAGAAGA 
CATTCTGTCC CTGGGACACC CACTCGTGGG TCTCCACGGC GACAGCGTGC AAACGCGTGC 
GGAAAATCTT CTGGTGGTCA GACCCAGGCG GGAGCGCCGG AGGAGGATGA TGATGATGGG 
CTGCCATCTC TAGACACAGA AGTCTGGAGC TGGGGACGGG GCTCTGAGGG GCAGCTCGGC 
CACGGAGATC AGCTCGCCAG ACTTCAGCCT CTGTGTATCA AGACACTGTC GGGTGAGGAG 
GTGATCCAAG TTGCAGCAGG GTCCCATCAT TCTCTGGCTG TCACTGCAAC CTGTCAGGTG 
TACTCATGGG GCAGCAACAT GTGTGGGCAG CTGGGTCACG ATAACAGTTC TTTCGTTGTG 
CCCCAACAGG CAAAGCTGTC TGATGGCCTC CGAGTTTGGG ACATGTCTGC CGGCCAGAGC 
CACTCCCTCC TCCTGGCTGA TGGAGACTGC GTCCAGCCCG TGTTGTTGTA CTGTGGCCGG 
CAGAAAGAGT CGACATCAGA CCAGAGAGAA AAGTCCTACC AGAGGTCCCC CAGAAAAGCA 
GAGCATTACA CAGTCAGGCC CACCCTGCTG CCTGTCTGCC TGGAGATGGG CTACATCAGC 
AGTGTGTGCA GCGGCGGGCA AAGCTGCGCC GTCCTGGCAG ACCAGAACAT CAAGGTTTTT 
ATTTCGGCCC TCCACGAGCT GGCCTCCAGA GAGAGGCAGT TCTACTGCTG GTTGAGTCGC 
GTCAGGAAAC TCGTCCTCAC ACCAATACAG AACAAAGAGA GTACGTCTCC GTTTTTAGGT 
GACCCTTGCA CCCATCTCTT TTCTTCTCTC TGTGGGAGTT TTTCTCAGCT AAGCGTCCTA 
ATCGGTCGAC ACTCCACATC GCTTACCTAC TTCCTGCAAA AGGCACAGGG CCGTGACGTC 
ACCTCACTCC CCCTGCTCAC ACACATGGAG CAGTTTCTGG ACACTTACAA AGAATATTGT 
TCCTCTGTTG GTGACTTTCA GGTGATGGGG GGCTTCCAGA CCCTCCATAA GCTCACACTT 
GAGTGTTTGC AGCCAGAAGT TCTCACTCAG CTGTGTATGT CGGACCAGAC CAGTGATGAC 
ACTGGTCTGG TTTCGCTGTT GTACTGGCCT CTGCAGCAGC TCCACCAGTA CGACCGAGTC 
ATGGTCAAAG TGGCATTTTG CTTCGACGTG TTAATGCCAG AGTTTCAGTC CTTCCGTCAG 
GGCTGCGGTC AGTACGAGTC CCTGTCTCTG TCCCTGTTGA GGAAGAAAAA AGAGGCTGAA 
AGCACATTCC TCTTCTGGAA GAGCCACTCC GGAAAAACTA CTGAGAGTCT ACGTCTCCCT 
CGGCGCCGAG TGGTGTGTGA AAGCAGCAGC AGATCTTTGA CTCTGCAAAA GGCCGGGCGC 
TTCTCCAACC ACTGGTTCAT TCTGTTCAAT GATGCACTGG TCCACACGCA GGGCGCGCTT 
CCCTCACAGC TCTTTTTCTC CACACATCAC GTCTACCCCC TGACCTGCCT GTGGGTGAAA 
CCCGTCACTG ACGACAGCAG CGGGCTCTAT GCCATTAAAA TTATTTGCCC GGAGGAAAGT 
TTCACCATGG TGGCTTCAAC ACCTCAAGAA AAGAACAAGT GGTTACGATC TATCAATCAC 
GCGCTGGATC AGGTGCTCGG TGGTAGTGGT GAGGGGTCGT CGCCGGGGAC GATGGGAATG 
TCCCGCACAG CCTCCTACAC ATTCACCGGA GAAGGCCGGT TTAAAGATGC CAAATACAGC 
GGCAGCTGGA TGTCAGGACG GGTGCAGGGC AGAGGGTCCT TGAGGTGGCC TGATGGGCGG 
ACCTACTGCG GGAACTTCAA GAACGGCCTT GAAGATGGTT ACGGGGAGTG CGTAATACCC 
AACAAAGAGC TGAACAAGCC TGACAGTTAC CATGGACACT GGAGAGAGGG GAAGATCCAC 
GGTTTCGGCA AATACAAGTA CGCCAGCGGC GAGGTGTATG AAGGCTGTTT CAGTGAAGGG 
CAGCGGCACG GTTACGGCAT GCTGAGCTCC GGGAAGCTGG GGAAGAAGTC CTCCAGTGTT 
TTCATCGGCC ACTGGGTCCA TGACAAGAAG ACAGGTTATG GAGTGTTTGA CGACATCACC 
AGGGGTGAGA AGTACATGGG ATTGTGGGTA GACGAGAAGC GGCACGGCAG CGGCGTGGTG 
GTCACACAGT ATGGCGTGTA CTACGAAGGA ACCTTCAGAG ACAACAAGAT GAGTGGTCCC 
GGTCTGCTGA TGTCGGATGA CGACACGGCT ATTCATGGGG AGTTTTCCGA CGATTGGGTT 
GTCAACGGGA AGGGTGTTTT ATTGTTGGCC AACGGAGACT GTCTGGAGGG TCTGTTCAGT 
GGGGAGTGGA CATCTGGACT GAAGGTGGAT GGAACGTACA CCAAACCACT GGTGGACGAG 
CCTGTAAACA AAGAAAGAAA AAACATGTTC AAGCTGGGTA GGTACGTGGT GCCTGCGGGT 
CAGAGGTGGG TCTGTGTGTT TGAGGAGTGC TGGAGTCGAC TGGGCTGCGA CAGCACTGGG 
AGAGGAGACA AAAATGTAGC CTGGGAGAAC ATCGCAGTGT CCATAACGGC TGCACGGAGG 
CAAAGTCCAG ATCTCAGTCG GTCTCAGAAC AAAGTGTTGG AGTCTCTGGA GTTTATCCCT 
CAGCATGAGC AGCCAGTCAC CACCTCGAAC TACGACAGCA TACGCAGATA CCTCATCAAG 
GCTTGTGAGA CCCCCCTCCA CCCTCTGGGC TGGCTGGTGG AGACTCTGGT GACGGTGTAC 
CGGATGACGT ATGTGGGCGT GGAAGCTAAC CGGAGGCTGC TGGAACAGGC TGTCCAGGAG 
CTTCAGGCCT ACCTCACACA TCTCTACAAC ATTGTCCAGT TTTTGTTTCC AGGGATGCCA 
GATGATGGCG CCGTCATCCC AGACCCCCCG GGGTCTCCAT CGGTAGTCCA GAACAGCTCC 
AGCATGGAAG ACTTGAGTGC TGTAGTGGTG AGCTGCTCCT CTCTGCTCCT CCCTCTGCTC 
CTGCCTCGAC TCTACCCTCC TCTCTTCACG CTGTACCGGC TGCAGGAGGA GCAGGAAGAC 
GCACTGTACT GGGACCGCAT CCTGCGTCTG AACAAACAAC CCGATCAGTC GCTGCTGAGC 
TTCCTGGGAG TGCAAGAGAA GTTCTGGCCT ACGTGGATGT CAATTTTGGG GGAGAAGAAG 
CAGATTGTGT CGAGCAGCAA AGACGCCTGC TTCGTTTCCG CAGTTGAGAC CCTCCAACAA 
ATCAGCACCA CCTTTACTCC ATCTGACAAA CTCGTCGTCA TCCAGAAGAC CTTTGAGGAG 
TTGACCCAGG AAGTCAATCC CATGCTGGAC GGCCACTTCC TGTGGTGCAT GGATGACTTG 
CTCCCACTCT TCATCTACGT GGTGGTCAGA GCGAGGATCA GGAATCTGGG AGCGGAGGTC 
GATATGATTG AAGATTTGAT GGATCCGAAT GTTCAGCATG GAGAGCTGGG ACTGATGTTC 
ACCACCCTGA AGGCCTGCTA CATCCAGATC AAAAACGAGT CTACGACATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101078293 ( XM_011611395.2 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_011609697.2 Takifugu rubripes alsin-like (LOC101078293), transcript variant X1, mRNA.

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CloneID	OTo42292
Clone ID Related Accession (Same CDS sequence)	XM_011611395.2
Accession Version	XM_011611395.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4710bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-04
Organism	Takifugu rubripes(torafugu)
Product	alsin-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042285.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jul 5, 2019 this sequence version replaced XM_011611395.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAACG CAGAGGACAG CCCAGCAGAT GAGTTGGTCT CCAGAGCTCC AGAGAGTGGA 
CTTTTCCACA TCTGGCAGTC AGCAGGCCCC AGTGCCCAGC TGTGTCCGGA GAGAGTGCTG 
CTGTCTATTC CTGTTGTGCA GGCTGCCCTG GGAGAGCATC ATGGAGTCCT CTTGGTGCAG 
GGGGGCCTCG TCTATTCATT TGGAGAGCTC TTGTGGAAAG ATCTGAGTGT CCCCGCATCT 
GCTCCAGTCT TGGAAGGCTC TCTGCTGGGA CGGGTGGTGG TCCGGGTGGC TGCAGGCGGT 
TTCCACTGTG GCGCTGTGAG CGAACAGGGA CATGTGTACA CATGGGGGGA GAACACTGGA 
GGACAGTGTG GGCTGACAGA GCGGACTACA GAAAAAACCA TCACAGTTTC AGAGCCTTCT 
CTTGTTAGCG TGGTCGACCA TGAGGTCATC CCGCCAGCGG CTGTTAGGGT TGTGGATCTT 
GCTTTTGGAC GCGAGCACAG TCTGGCTCTG TCAGCCCAGA ACGAGCTGTG GGCGTGGGGC 
AGCGGCTGCC AGCTTGGCCT GGTCACCACC ACCTTCCCTG TGTGTAGACC ACAGAAGGTG 
GAGCATTTAG CAGGCAGACA TGTTATTCAG GTTGTTTGCG GTGCCTACCA CAGCTTGGCC 
CTGGTTCGTA GTTTGCCTCC AAATAGCTAC AACAGCCAGA AGCCTTCTGA AAAAAGGGAA 
CGGGGTCACT CGCCGCACTA TTCAGCAGCA GAGAGAGAGG AGCTGTTTGT GGGTGACAGC 
AGTCTCTACT GTCCACTTGG GGTGGAGCTG AAAGAAGGCA TGGCCACAGA GACTTCCTCC 
AGGAGATGTC CAAGGAGAAG GCTCCATCCT GGAGGAAGTT CGGCCAGCAG CAGCCCTGGC 
TCTGCATTTA ACGAAGACGC GACCAAAGAC ACTGACTCAC TGAGCAGCTG CACCTCAGAT 
GACAGCTGTG TTTCCTCAAC TCTCTCCACT GATATGCTGA CCTCCAGTTA CAAAGAACCT 
AACAATAACT TTGGAGTGAC CCTGCCGTGC TCGTCCTCCT CACTGTGTCT CGACGATGTC 
CGACTCTGTC TTGAATCGGG GCTGCAGGTA CAGCCAGGGA AGAAGCTCTC CAGCCTCTCC 
AATAAACACT ATAAAGCAAA ATCAGCAGGA ACCAGAAGAC ATTCTGTCCC TGGGACACCC 
ACTCGTGGGT CTCCACGGCG ACAGCGTGCA AACGCGTGCG GAAAATCTTC TGGTGGTCAG 
ACCCAGGCGG GAGCGCCGGA GGAGGATGAT GATGATGGGC TGCCATCTCT AGACACAGAA 
GTCTGGAGCT GGGGACGGGG CTCTGAGGGG CAGCTCGGCC ACGGAGATCA GCTCGCCAGA 
CTTCAGCCTC TGTGTATCAA GACACTGTCG GGTGAGGAGG TGATCCAAGT TGCAGCAGGG 
TCCCATCATT CTCTGGCTGT CACTGCAACC TGTCAGGTGT ACTCATGGGG CAGCAACATG 
TGTGGGCAGC TGGGTCACGA TAACAGTTCT TTCGTTGTGC CCCAACAGGC AAAGCTGTCT 
GATGGCCTCC GAGTTTGGGA CATGTCTGCC GGCCAGAGCC ACTCCCTCCT CCTGGCTGAT 
GGAGACTGCG TCCAGCCCGT GTTGTTGTAC TGTGGCCGGC AGAAAGAGTC GACATCAGAC 
CAGAGAGAAA AGTCCTACCA GAGGTCCCCC AGAAAAGCAG AGCATTACAC AGTCAGGCCC 
ACCCTGCTGC CTGTCTGCCT GGAGATGGGC TACATCAGCA GTGTGTGCAG CGGCGGGCAA 
AGCTGCGCCG TCCTGGCAGA CCAGAACATC AAGGTTTTTA TTTCGGCCCT CCACGAGCTG 
GCCTCCAGAG AGAGGCAGTT CTACTGCTGG TTGAGTCGCG TCAGGAAACT CGTCCTCACA 
CCAATACAGA ACAAAGAGAG TACGTCTCCG TTTTTAGGTG ACCCTTGCAC CCATCTCTTT 
TCTTCTCTCT GTGGGAGTTT TTCTCAGCTA AGCGTCCTAA TCGGTCGACA CTCCACATCG 
CTTACCTACT TCCTGCAAAA GGCACAGGGC CGTGACGTCA CCTCACTCCC CCTGCTCACA 
CACATGGAGC AGTTTCTGGA CACTTACAAA GAATATTGTT CCTCTGTTGG TGACTTTCAG 
GTGATGGGGG GCTTCCAGAC CCTCCATAAG CTCACACTTG AGTGTTTGCA GCCAGAAGTT 
CTCACTCAGC TGTGTATGTC GGACCAGACC AGTGATGACA CTGGTCTGGT TTCGCTGTTG 
TACTGGCCTC TGCAGCAGCT CCACCAGTAC GACCGAGTCA TGGTCAAAGT GGCATTTTGC 
TTCGACGTGT TAATGCCAGA GTTTCAGTCC TTCCGTCAGG GCTGCGGTCA GTACGAGTCC 
CTGTCTCTGT CCCTGTTGAG GAAGAAAAAA GAGGCTGAAA GCACATTCCT CTTCTGGAAG 
AGCCACTCCG GAAAAACTAC TGAGAGTCTA CGTCTCCCTC GGCGCCGAGT GGTGTGTGAA 
AGCAGCAGCA GATCTTTGAC TCTGCAAAAG GCCGGGCGCT TCTCCAACCA CTGGTTCATT 
CTGTTCAATG ATGCACTGGT CCACACGCAG GGCGCGCTTC CCTCACAGCT CTTTTTCTCC 
ACACATCACG TCTACCCCCT GACCTGCCTG TGGGTGAAAC CCGTCACTGA CGACAGCAGC 
GGGCTCTATG CCATTAAAAT TATTTGCCCG GAGGAAAGTT TCACCATGGT GGCTTCAACA 
CCTCAAGAAA AGAACAAGTG GTTACGATCT ATCAATCACG CGCTGGATCA GGTGCTCGGT 
GGTAGTGGTG AGGGGTCGTC GCCGGGGACG ATGGGAATGT CCCGCACAGC CTCCTACACA 
TTCACCGGAG AAGGCCGGTT TAAAGATGCC AAATACAGCG GCAGCTGGAT GTCAGGACGG 
GTGCAGGGCA GAGGGTCCTT GAGGTGGCCT GATGGGCGGA CCTACTGCGG GAACTTCAAG 
AACGGCCTTG AAGATGGTTA CGGGGAGTGC GTAATACCCA ACAAAGAGCT GAACAAGCCT 
GACAGTTACC ATGGACACTG GAGAGAGGGG AAGATCCACG GTTTCGGCAA ATACAAGTAC 
GCCAGCGGCG AGGTGTATGA AGGCTGTTTC AGTGAAGGGC AGCGGCACGG TTACGGCATG 
CTGAGCTCCG GGAAGCTGGG GAAGAAGTCC TCCAGTGTTT TCATCGGCCA CTGGGTCCAT 
GACAAGAAGA CAGGTTATGG AGTGTTTGAC GACATCACCA GGGGTGAGAA GTACATGGGA 
TTGTGGGTAG ACGAGAAGCG GCACGGCAGC GGCGTGGTGG TCACACAGTA TGGCGTGTAC 
TACGAAGGAA CCTTCAGAGA CAACAAGATG AGTGGTCCCG GTCTGCTGAT GTCGGATGAC 
GACACGGCTA TTCATGGGGA GTTTTCCGAC GATTGGGTTG TCAACGGGAA GGGTGTTTTA 
TTGTTGGCCA ACGGAGACTG TCTGGAGGGT CTGTTCAGTG GGGAGTGGAC ATCTGGACTG 
AAGGTGGATG GAACGTACAC CAAACCACTG GTGGACGAGC CTGTAAACAA AGAAAGAAAA 
AACATGTTCA AGCTGGGTAG GTACGTGGTG CCTGCGGGTC AGAGGTGGGT CTGTGTGTTT 
GAGGAGTGCT GGAGTCGACT GGGCTGCGAC AGCACTGGGA GAGGAGACAA AAATGTAGCC 
TGGGAGAACA TCGCAGTGTC CATAACGGCT GCACGGAGGC AAAGTCCAGA TCTCAGTCGG 
TCTCAGAACA AAGTGTTGGA GTCTCTGGAG TTTATCCCTC AGCATGAGCA GCCAGTCACC 
ACCTCGAACT ACGACAGCAT ACGCAGATAC CTCATCAAGG CTTGTGAGAC CCCCCTCCAC 
CCTCTGGGCT GGCTGGTGGA GACTCTGGTG ACGGTGTACC GGATGACGTA TGTGGGCGTG 
GAAGCTAACC GGAGGCTGCT GGAACAGGCT GTCCAGGAGC TTCAGGCCTA CCTCACACAT 
CTCTACAACA TTGTCCAGTT TTTGTTTCCA GGGATGCCAG ATGATGGCGC CGTCATCCCA 
GACCCCCCGG GGTCTCCATC GGTAGTCCAG AACAGCTCCA GCATGGAAGA CTTGAGTGCT 
GTAGTGGTGA GCTGCTCCTC TCTGCTCCTC CCTCTGCTCC TGCCTCGACT CTACCCTCCT 
CTCTTCACGC TGTACCGGCT GCAGGAGGAG CAGGAAGACG CACTGTACTG GGACCGCATC 
CTGCGTCTGA ACAAACAACC CGATCAGTCG CTGCTGAGCT TCCTGGGAGT GCAAGAGAAG 
TTCTGGCCTA CGTGGATGTC AATTTTGGGG GAGAAGAAGC AGATTGTGTC GAGCAGCAAA 
GACGCCTGCT TCGTTTCCGC AGTTGAGACC CTCCAACAAA TCAGCACCAC CTTTACTCCA 
TCTGACAAAC TCGTCGTCAT CCAGAAGACC TTTGAGGAGT TGACCCAGGA AGTCAATCCC 
ATGCTGGACG GCCACTTCCT GTGGTGCATG GATGACTTGC TCCCACTCTT CATCTACGTG 
GTGGTCAGAG CGAGGATCAG GAATCTGGGA GCGGAGGTCG ATATGATTGA AGATTTGATG 
GATCCGAATG TTCAGCATGG AGAGCTGGGA CTGATGTTCA CCACCCTGAA GGCCTGCTAC 
ATCCAGATCA AAAACGAGTC TACGACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011609697.2
CDS	246..4955
Translation	MENAEDSPADELVSRAPESGLFHIWQSAGPSAQLCPERVLLSIPVVQAALGEHHGVLLVQGGLVYSFGELLWKDLSVPASAPVLEGSLLGRVVVRVAAGGFHCGAVSEQGHVYTWGENTGGQCGLTERTTEKTITVSEPSLVSVVDHEVIPPAAVRVVDLAFGREHSLALSAQNELWAWGSGCQLGLVTTTFPVCRPQKVEHLAGRHVIQVVCGAYHSLALVRSLPPNSYNSQKPSEKRERGHSPHYSAAEREELFVGDSSLYCPLGVELKEGMATETSSRRCPRRRLHPGGSSASSSPGSAFNEDATKDTDSLSSCTSDDSCVSSTLSTDMLTSSYKEPNNNFGVTLPCSSSSLCLDDVRLCLESGLQVQPGKKLSSLSNKHYKAKSAGTRRHSVPGTPTRGSPRRQRANACGKSSGGQTQAGAPEEDDDDGLPSLDTEVWSWGRGSEGQLGHGDQLARLQPLCIKTLSGEEVIQVAAGSHHSLAVTATCQVYSWGSNMCGQLGHDNSSFVVPQQAKLSDGLRVWDMSAGQSHSLLLADGDCVQPVLLYCGRQKESTSDQREKSYQRSPRKAEHYTVRPTLLPVCLEMGYISSVCSGGQSCAVLADQNIKVFISALHELASRERQFYCWLSRVRKLVLTPIQNKESTSPFLGDPCTHLFSSLCGSFSQLSVLIGRHSTSLTYFLQKAQGRDVTSLPLLTHMEQFLDTYKEYCSSVGDFQVMGGFQTLHKLTLECLQPEVLTQLCMSDQTSDDTGLVSLLYWPLQQLHQYDRVMVKVAFCFDVLMPEFQSFRQGCGQYESLSLSLLRKKKEAESTFLFWKSHSGKTTESLRLPRRRVVCESSSRSLTLQKAGRFSNHWFILFNDALVHTQGALPSQLFFSTHHVYPLTCLWVKPVTDDSSGLYAIKIICPEESFTMVASTPQEKNKWLRSINHALDQVLGGSGEGSSPGTMGMSRTASYTFTGEGRFKDAKYSGSWMSGRVQGRGSLRWPDGRTYCGNFKNGLEDGYGECVIPNKELNKPDSYHGHWREGKIHGFGKYKYASGEVYEGCFSEGQRHGYGMLSSGKLGKKSSSVFIGHWVHDKKTGYGVFDDITRGEKYMGLWVDEKRHGSGVVVTQYGVYYEGTFRDNKMSGPGLLMSDDDTAIHGEFSDDWVVNGKGVLLLANGDCLEGLFSGEWTSGLKVDGTYTKPLVDEPVNKERKNMFKLGRYVVPAGQRWVCVFEECWSRLGCDSTGRGDKNVAWENIAVSITAARRQSPDLSRSQNKVLESLEFIPQHEQPVTTSNYDSIRRYLIKACETPLHPLGWLVETLVTVYRMTYVGVEANRRLLEQAVQELQAYLTHLYNIVQFLFPGMPDDGAVIPDPPGSPSVVQNSSSMEDLSAVVVSCSSLLLPLLLPRLYPPLFTLYRLQEEQEDALYWDRILRLNKQPDQSLLSFLGVQEKFWPTWMSILGEKKQIVSSSKDACFVSAVETLQQISTTFTPSDKLVVIQKTFEELTQEVNPMLDGHFLWCMDDLLPLFIYVVVRARIRNLGAEVDMIEDLMDPNVQHGELGLMFTTLKACYIQIKNESTT

Target ORF information:

RefSeq Version	XM_011611395.2
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes alsin-like (LOC101078293), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011611395.2

ORF Insert Sequence:

ATGGAGAACG CAGAGGACAG CCCAGCAGAT GAGTTGGTCT CCAGAGCTCC AGAGAGTGGA 
CTTTTCCACA TCTGGCAGTC AGCAGGCCCC AGTGCCCAGC TGTGTCCGGA GAGAGTGCTG 
CTGTCTATTC CTGTTGTGCA GGCTGCCCTG GGAGAGCATC ATGGAGTCCT CTTGGTGCAG 
GGGGGCCTCG TCTATTCATT TGGAGAGCTC TTGTGGAAAG ATCTGAGTGT CCCCGCATCT 
GCTCCAGTCT TGGAAGGCTC TCTGCTGGGA CGGGTGGTGG TCCGGGTGGC TGCAGGCGGT 
TTCCACTGTG GCGCTGTGAG CGAACAGGGA CATGTGTACA CATGGGGGGA GAACACTGGA 
GGACAGTGTG GGCTGACAGA GCGGACTACA GAAAAAACCA TCACAGTTTC AGAGCCTTCT 
CTTGTTAGCG TGGTCGACCA TGAGGTCATC CCGCCAGCGG CTGTTAGGGT TGTGGATCTT 
GCTTTTGGAC GCGAGCACAG TCTGGCTCTG TCAGCCCAGA ACGAGCTGTG GGCGTGGGGC 
AGCGGCTGCC AGCTTGGCCT GGTCACCACC ACCTTCCCTG TGTGTAGACC ACAGAAGGTG 
GAGCATTTAG CAGGCAGACA TGTTATTCAG GTTGTTTGCG GTGCCTACCA CAGCTTGGCC 
CTGGTTCGTA GTTTGCCTCC AAATAGCTAC AACAGCCAGA AGCCTTCTGA AAAAAGGGAA 
CGGGGTCACT CGCCGCACTA TTCAGCAGCA GAGAGAGAGG AGCTGTTTGT GGGTGACAGC 
AGTCTCTACT GTCCACTTGG GGTGGAGCTG AAAGAAGGCA TGGCCACAGA GACTTCCTCC 
AGGAGATGTC CAAGGAGAAG GCTCCATCCT GGAGGAAGTT CGGCCAGCAG CAGCCCTGGC 
TCTGCATTTA ACGAAGACGC GACCAAAGAC ACTGACTCAC TGAGCAGCTG CACCTCAGAT 
GACAGCTGTG TTTCCTCAAC TCTCTCCACT GATATGCTGA CCTCCAGTTA CAAAGAACCT 
AACAATAACT TTGGAGTGAC CCTGCCGTGC TCGTCCTCCT CACTGTGTCT CGACGATGTC 
CGACTCTGTC TTGAATCGGG GCTGCAGGTA CAGCCAGGGA AGAAGCTCTC CAGCCTCTCC 
AATAAACACT ATAAAGCAAA ATCAGCAGGA ACCAGAAGAC ATTCTGTCCC TGGGACACCC 
ACTCGTGGGT CTCCACGGCG ACAGCGTGCA AACGCGTGCG GAAAATCTTC TGGTGGTCAG 
ACCCAGGCGG GAGCGCCGGA GGAGGATGAT GATGATGGGC TGCCATCTCT AGACACAGAA 
GTCTGGAGCT GGGGACGGGG CTCTGAGGGG CAGCTCGGCC ACGGAGATCA GCTCGCCAGA 
CTTCAGCCTC TGTGTATCAA GACACTGTCG GGTGAGGAGG TGATCCAAGT TGCAGCAGGG 
TCCCATCATT CTCTGGCTGT CACTGCAACC TGTCAGGTGT ACTCATGGGG CAGCAACATG 
TGTGGGCAGC TGGGTCACGA TAACAGTTCT TTCGTTGTGC CCCAACAGGC AAAGCTGTCT 
GATGGCCTCC GAGTTTGGGA CATGTCTGCC GGCCAGAGCC ACTCCCTCCT CCTGGCTGAT 
GGAGACTGCG TCCAGCCCGT GTTGTTGTAC TGTGGCCGGC AGAAAGAGTC GACATCAGAC 
CAGAGAGAAA AGTCCTACCA GAGGTCCCCC AGAAAAGCAG AGCATTACAC AGTCAGGCCC 
ACCCTGCTGC CTGTCTGCCT GGAGATGGGC TACATCAGCA GTGTGTGCAG CGGCGGGCAA 
AGCTGCGCCG TCCTGGCAGA CCAGAACATC AAGGTTTTTA TTTCGGCCCT CCACGAGCTG 
GCCTCCAGAG AGAGGCAGTT CTACTGCTGG TTGAGTCGCG TCAGGAAACT CGTCCTCACA 
CCAATACAGA ACAAAGAGAG TACGTCTCCG TTTTTAGGTG ACCCTTGCAC CCATCTCTTT 
TCTTCTCTCT GTGGGAGTTT TTCTCAGCTA AGCGTCCTAA TCGGTCGACA CTCCACATCG 
CTTACCTACT TCCTGCAAAA GGCACAGGGC CGTGACGTCA CCTCACTCCC CCTGCTCACA 
CACATGGAGC AGTTTCTGGA CACTTACAAA GAATATTGTT CCTCTGTTGG TGACTTTCAG 
GTGATGGGGG GCTTCCAGAC CCTCCATAAG CTCACACTTG AGTGTTTGCA GCCAGAAGTT 
CTCACTCAGC TGTGTATGTC GGACCAGACC AGTGATGACA CTGGTCTGGT TTCGCTGTTG 
TACTGGCCTC TGCAGCAGCT CCACCAGTAC GACCGAGTCA TGGTCAAAGT GGCATTTTGC 
TTCGACGTGT TAATGCCAGA GTTTCAGTCC TTCCGTCAGG GCTGCGGTCA GTACGAGTCC 
CTGTCTCTGT CCCTGTTGAG GAAGAAAAAA GAGGCTGAAA GCACATTCCT CTTCTGGAAG 
AGCCACTCCG GAAAAACTAC TGAGAGTCTA CGTCTCCCTC GGCGCCGAGT GGTGTGTGAA 
AGCAGCAGCA GATCTTTGAC TCTGCAAAAG GCCGGGCGCT TCTCCAACCA CTGGTTCATT 
CTGTTCAATG ATGCACTGGT CCACACGCAG GGCGCGCTTC CCTCACAGCT CTTTTTCTCC 
ACACATCACG TCTACCCCCT GACCTGCCTG TGGGTGAAAC CCGTCACTGA CGACAGCAGC 
GGGCTCTATG CCATTAAAAT TATTTGCCCG GAGGAAAGTT TCACCATGGT GGCTTCAACA 
CCTCAAGAAA AGAACAAGTG GTTACGATCT ATCAATCACG CGCTGGATCA GGTGCTCGGT 
GGTAGTGGTG AGGGGTCGTC GCCGGGGACG ATGGGAATGT CCCGCACAGC CTCCTACACA 
TTCACCGGAG AAGGCCGGTT TAAAGATGCC AAATACAGCG GCAGCTGGAT GTCAGGACGG 
GTGCAGGGCA GAGGGTCCTT GAGGTGGCCT GATGGGCGGA CCTACTGCGG GAACTTCAAG 
AACGGCCTTG AAGATGGTTA CGGGGAGTGC GTAATACCCA ACAAAGAGCT GAACAAGCCT 
GACAGTTACC ATGGACACTG GAGAGAGGGG AAGATCCACG GTTTCGGCAA ATACAAGTAC 
GCCAGCGGCG AGGTGTATGA AGGCTGTTTC AGTGAAGGGC AGCGGCACGG TTACGGCATG 
CTGAGCTCCG GGAAGCTGGG GAAGAAGTCC TCCAGTGTTT TCATCGGCCA CTGGGTCCAT 
GACAAGAAGA CAGGTTATGG AGTGTTTGAC GACATCACCA GGGGTGAGAA GTACATGGGA 
TTGTGGGTAG ACGAGAAGCG GCACGGCAGC GGCGTGGTGG TCACACAGTA TGGCGTGTAC 
TACGAAGGAA CCTTCAGAGA CAACAAGATG AGTGGTCCCG GTCTGCTGAT GTCGGATGAC 
GACACGGCTA TTCATGGGGA GTTTTCCGAC GATTGGGTTG TCAACGGGAA GGGTGTTTTA 
TTGTTGGCCA ACGGAGACTG TCTGGAGGGT CTGTTCAGTG GGGAGTGGAC ATCTGGACTG 
AAGGTGGATG GAACGTACAC CAAACCACTG GTGGACGAGC CTGTAAACAA AGAAAGAAAA 
AACATGTTCA AGCTGGGTAG GTACGTGGTG CCTGCGGGTC AGAGGTGGGT CTGTGTGTTT 
GAGGAGTGCT GGAGTCGACT GGGCTGCGAC AGCACTGGGA GAGGAGACAA AAATGTAGCC 
TGGGAGAACA TCGCAGTGTC CATAACGGCT GCACGGAGGC AAAGTCCAGA TCTCAGTCGG 
TCTCAGAACA AAGTGTTGGA GTCTCTGGAG TTTATCCCTC AGCATGAGCA GCCAGTCACC 
ACCTCGAACT ACGACAGCAT ACGCAGATAC CTCATCAAGG CTTGTGAGAC CCCCCTCCAC 
CCTCTGGGCT GGCTGGTGGA GACTCTGGTG ACGGTGTACC GGATGACGTA TGTGGGCGTG 
GAAGCTAACC GGAGGCTGCT GGAACAGGCT GTCCAGGAGC TTCAGGCCTA CCTCACACAT 
CTCTACAACA TTGTCCAGTT TTTGTTTCCA GGGATGCCAG ATGATGGCGC CGTCATCCCA 
GACCCCCCGG GGTCTCCATC GGTAGTCCAG AACAGCTCCA GCATGGAAGA CTTGAGTGCT 
GTAGTGGTGA GCTGCTCCTC TCTGCTCCTC CCTCTGCTCC TGCCTCGACT CTACCCTCCT 
CTCTTCACGC TGTACCGGCT GCAGGAGGAG CAGGAAGACG CACTGTACTG GGACCGCATC 
CTGCGTCTGA ACAAACAACC CGATCAGTCG CTGCTGAGCT TCCTGGGAGT GCAAGAGAAG 
TTCTGGCCTA CGTGGATGTC AATTTTGGGG GAGAAGAAGC AGATTGTGTC GAGCAGCAAA 
GACGCCTGCT TCGTTTCCGC AGTTGAGACC CTCCAACAAA TCAGCACCAC CTTTACTCCA 
TCTGACAAAC TCGTCGTCAT CCAGAAGACC TTTGAGGAGT TGACCCAGGA AGTCAATCCC 
ATGCTGGACG GCCACTTCCT GTGGTGCATG GATGACTTGC TCCCACTCTT CATCTACGTG 
GTGGTCAGAG CGAGGATCAG GAATCTGGGA GCGGAGGTCG ATATGATTGA AGATTTGATG 
GATCCGAATG TTCAGCATGG AGAGCTGGGA CTGATGTTCA CCACCCTGAA GGCCTGCTAC 
ATCCAGATCA AAAACGAGTC TACGACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101078293 ( XM_011611399.2 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_011609701.2 Takifugu rubripes alsin-like (LOC101078293), transcript variant X2, mRNA.

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CloneID	OTo42293
Clone ID Related Accession (Same CDS sequence)	XM_011611399.2
Accession Version	XM_011611399.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4686bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-04
Organism	Takifugu rubripes(torafugu)
Product	alsin-like isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042285.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jul 5, 2019 this sequence version replaced XM_011611399.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAACG CAGAGGACAG CCCAGCAGAT GAGTTGGTCT CCAGAGCTCC AGAGAGTGGA 
CTTTTCCACA TCTGGCAGTC AGCAGGCCCC AGTGCCCAGC TGTGTCCGGA GAGAGTGCTG 
CTGTCTATTC CTGTTGTGCA GGCTGCCCTG GGAGAGCATC ATGGAGTCCT CTTGGTGCAG 
GGGGGCCTCG TCTATTCATT TGGAGAGCTC TTGTGGAAAG ATCTGAGTGT CCCCGCATCT 
GCTCCAGTCT TGGAAGGCTC TCTGCTGGGA CGGGTGGTGG TCCGGGTGGC TGCAGGCGGT 
TTCCACTGTG GCGCTGTGAG CGAACAGGGA CATGTGTACA CATGGGGGGA GAACACTGGA 
GGACAGTGTG GGCTGACAGA GCGGACTACA GAAAAAACCA TCACAGTTTC AGAGCCTTCT 
CTTGTTAGCG TGGTCGACCA TGAGGTCATC CCGCCAGCGG CTGTTAGGGT TGTGGATCTT 
GCTTTTGGAC GCGAGCACAG TCTGGCTCTG TCAGCCCAGA ACGAGCTGTG GGCGTGGGGC 
AGCGGCTGCC AGCTTGGCCT GGTCACCACC ACCTTCCCTG TGTGTAGACC ACAGAAGGTG 
GAGCATTTAG CAGGCAGACA TGTTATTCAG GTTGTTTGCG GTGCCTACCA CAGCTTGGCC 
CTGGTTCGTA GTTTGCCTCC AAATAGCTAC AACAGCCAGA AGCCTTCTGA AAAAAGGGAA 
CGGGGTCACT CGCCGCACTA TTCAGCAGCA GAGAGAGAGG AGCTGTTTGT GGGTGACAGC 
AGTCTCTACT GTCCACTTGG GGTGGAGCTG AAAGAAGGCA TGGCCACAGA GACTTCCTCC 
AGGAGATGTC CAAGGAGAAG GCTCCATCCT GGAGGAAGTT CGGCCAGCAG CAGCCCTGGC 
TCTGCATTTA ACGAAGACGC GACCAAAGAC ACTGACTCAC TGAGCAGCTG CACCTCAGAT 
GACAGCTGTG TTTCCTCAAC TCTCTCCACT GATATGCTGA CCTCCAGTTA CAAAGAACCT 
AACAATAACT TTGGAGTGAC CCTGCCGTGC TCGTCCTCCT CACTGTGTCT CGACGATGTC 
CGACTCTGTC TTGAATCGGG GCTGCAGGTA CAGCCAGGGA AGAAGCTCTC CAGCCTCTCC 
AATAAACACT ATAAAGCAAA ATCAGCAGGA ACCAGAAGAC ATTCTGTCCC TGGGACACCC 
ACTCGTGGGT CTCCACGGCG ACAGCGTGCA AACGCGTGCG GAAAATCTTC TGGTGGTCAG 
ACCCAGGCGG GAGCGCCGGA GGAGGATGAT GATGATGGGC TGCCATCTCT AGACACAGAA 
GTCTGGAGCT GGGGACGGGG CTCTGAGGGG CAGCTCGGCC ACGGAGATCA GCTCGCCAGA 
CTTCAGCCTC TGTGTATCAA GACACTGTCG GGTGAGGAGG TGATCCAAGT TGCAGCAGGG 
TCCCATCATT CTCTGGCTGT CACTGCAACC TGTCAGGTGT ACTCATGGGG CAGCAACATG 
TGTGGGCAGC TGGGTCACGA TAACAGTTCT TTCGTTGTGC CCCAACAGGC AAAGCTGTCT 
GATGGCCTCC GAGTTTGGGA CATGTCTGCC GGCCAGAGCC ACTCCCTCCT CCTGGCTGAT 
GGAGACTGCG TCCAGCCCGT GTTGTTGTAC TGTGGCCGGC AGAAAGAGTC GACATCAGAC 
CAGAGAGAAA AGTCCTACCA GAGGTCCCCC AGAAAAGCAG AGCATTACAC AGTCAGGCCC 
ACCCTGCTGC CTGTCTGCCT GGAGATGGGC TACATCAGCA GTGTGTGCAG CGGCGGGCAA 
AGCTGCGCCG TCCTGGCAGA CCAGAACATC AAGGTTTTTA TTTCGGCCCT CCACGAGCTG 
GCCTCCAGAG AGAGGCAGTT CTACTGCTGG TTGAGTCGCG TCAGGAAACT CGTCCTCACA 
CCAATACAGA ACAAAGAGAG TACGTCTCCG TTTTTAGGTG ACCCTTGCAC CCATCTCTTT 
TCTTCTCTCT GTGGGAGTTT TTCTCAGCTA AGCGTCCTAA TCGGTCGACA CTCCACATCG 
CTTACCTACT TCCTGCAAAA GGCACAGGGC CGTGACGTCA CCTCACTCCC CCTGCTCACA 
CACATGGAGC AGTTTCTGGA CACTTACAAA GAATATTGTT CCTCTGTTGG TGACTTTCAG 
GTGATGGGGG GCTTCCAGAC CCTCCATAAG CTCACACTTG AGTGTTTGCA GCCAGAAGTT 
CTCACTCAGC TGTGTATGTC GGACCAGACC AGTGATGACA CTGGTCTGGT TTCGCTGTTG 
TACTGGCCTC TGCAGCAGCT CCACCAGTAC GACCGAGTCA TGGTCAAAGT GGCATTTTGC 
TTCGACGTGT TAATGCCAGA GTTTCAGTCC TTCCGTCAGG GCTGCGGTCA GTACGAGTCC 
CTGTCTCTGT CCCTGTTGAG GAAGAAAAAA GAGGCTGAAA GCACATTCCT CTTCTGGAAG 
AGCCACTCCG GAAAAACTAC TGAGAGTCTA CGTCTCCCTC GGCGCCGAGT GGTGTGTGAA 
AGCAGCAGCA GATCTTTGAC TCTGCAAAAG GCCGGGCGCT TCTCCAACCA CTGGTTCATT 
CTGTTCAATG ATGCACTGGT CCACACGCAG TTCTCCACAC ATCACGTCTA CCCCCTGACC 
TGCCTGTGGG TGAAACCCGT CACTGACGAC AGCAGCGGGC TCTATGCCAT TAAAATTATT 
TGCCCGGAGG AAAGTTTCAC CATGGTGGCT TCAACACCTC AAGAAAAGAA CAAGTGGTTA 
CGATCTATCA ATCACGCGCT GGATCAGGTG CTCGGTGGTA GTGGTGAGGG GTCGTCGCCG 
GGGACGATGG GAATGTCCCG CACAGCCTCC TACACATTCA CCGGAGAAGG CCGGTTTAAA 
GATGCCAAAT ACAGCGGCAG CTGGATGTCA GGACGGGTGC AGGGCAGAGG GTCCTTGAGG 
TGGCCTGATG GGCGGACCTA CTGCGGGAAC TTCAAGAACG GCCTTGAAGA TGGTTACGGG 
GAGTGCGTAA TACCCAACAA AGAGCTGAAC AAGCCTGACA GTTACCATGG ACACTGGAGA 
GAGGGGAAGA TCCACGGTTT CGGCAAATAC AAGTACGCCA GCGGCGAGGT GTATGAAGGC 
TGTTTCAGTG AAGGGCAGCG GCACGGTTAC GGCATGCTGA GCTCCGGGAA GCTGGGGAAG 
AAGTCCTCCA GTGTTTTCAT CGGCCACTGG GTCCATGACA AGAAGACAGG TTATGGAGTG 
TTTGACGACA TCACCAGGGG TGAGAAGTAC ATGGGATTGT GGGTAGACGA GAAGCGGCAC 
GGCAGCGGCG TGGTGGTCAC ACAGTATGGC GTGTACTACG AAGGAACCTT CAGAGACAAC 
AAGATGAGTG GTCCCGGTCT GCTGATGTCG GATGACGACA CGGCTATTCA TGGGGAGTTT 
TCCGACGATT GGGTTGTCAA CGGGAAGGGT GTTTTATTGT TGGCCAACGG AGACTGTCTG 
GAGGGTCTGT TCAGTGGGGA GTGGACATCT GGACTGAAGG TGGATGGAAC GTACACCAAA 
CCACTGGTGG ACGAGCCTGT AAACAAAGAA AGAAAAAACA TGTTCAAGCT GGGTAGGTAC 
GTGGTGCCTG CGGGTCAGAG GTGGGTCTGT GTGTTTGAGG AGTGCTGGAG TCGACTGGGC 
TGCGACAGCA CTGGGAGAGG AGACAAAAAT GTAGCCTGGG AGAACATCGC AGTGTCCATA 
ACGGCTGCAC GGAGGCAAAG TCCAGATCTC AGTCGGTCTC AGAACAAAGT GTTGGAGTCT 
CTGGAGTTTA TCCCTCAGCA TGAGCAGCCA GTCACCACCT CGAACTACGA CAGCATACGC 
AGATACCTCA TCAAGGCTTG TGAGACCCCC CTCCACCCTC TGGGCTGGCT GGTGGAGACT 
CTGGTGACGG TGTACCGGAT GACGTATGTG GGCGTGGAAG CTAACCGGAG GCTGCTGGAA 
CAGGCTGTCC AGGAGCTTCA GGCCTACCTC ACACATCTCT ACAACATTGT CCAGTTTTTG 
TTTCCAGGGA TGCCAGATGA TGGCGCCGTC ATCCCAGACC CCCCGGGGTC TCCATCGGTA 
GTCCAGAACA GCTCCAGCAT GGAAGACTTG AGTGCTGTAG TGGTGAGCTG CTCCTCTCTG 
CTCCTCCCTC TGCTCCTGCC TCGACTCTAC CCTCCTCTCT TCACGCTGTA CCGGCTGCAG 
GAGGAGCAGG AAGACGCACT GTACTGGGAC CGCATCCTGC GTCTGAACAA ACAACCCGAT 
CAGTCGCTGC TGAGCTTCCT GGGAGTGCAA GAGAAGTTCT GGCCTACGTG GATGTCAATT 
TTGGGGGAGA AGAAGCAGAT TGTGTCGAGC AGCAAAGACG CCTGCTTCGT TTCCGCAGTT 
GAGACCCTCC AACAAATCAG CACCACCTTT ACTCCATCTG ACAAACTCGT CGTCATCCAG 
AAGACCTTTG AGGAGTTGAC CCAGGAAGTC AATCCCATGC TGGACGGCCA CTTCCTGTGG 
TGCATGGATG ACTTGCTCCC ACTCTTCATC TACGTGGTGG TCAGAGCGAG GATCAGGAAT 
CTGGGAGCGG AGGTCGATAT GATTGAAGAT TTGATGGATC CGAATGTTCA GCATGGAGAG 
CTGGGACTGA TGTTCACCAC CCTGAAGGCC TGCTACATCC AGATCAAAAA CGAGTCTACG 
ACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011609701.2
CDS	246..4931
Translation	MENAEDSPADELVSRAPESGLFHIWQSAGPSAQLCPERVLLSIPVVQAALGEHHGVLLVQGGLVYSFGELLWKDLSVPASAPVLEGSLLGRVVVRVAAGGFHCGAVSEQGHVYTWGENTGGQCGLTERTTEKTITVSEPSLVSVVDHEVIPPAAVRVVDLAFGREHSLALSAQNELWAWGSGCQLGLVTTTFPVCRPQKVEHLAGRHVIQVVCGAYHSLALVRSLPPNSYNSQKPSEKRERGHSPHYSAAEREELFVGDSSLYCPLGVELKEGMATETSSRRCPRRRLHPGGSSASSSPGSAFNEDATKDTDSLSSCTSDDSCVSSTLSTDMLTSSYKEPNNNFGVTLPCSSSSLCLDDVRLCLESGLQVQPGKKLSSLSNKHYKAKSAGTRRHSVPGTPTRGSPRRQRANACGKSSGGQTQAGAPEEDDDDGLPSLDTEVWSWGRGSEGQLGHGDQLARLQPLCIKTLSGEEVIQVAAGSHHSLAVTATCQVYSWGSNMCGQLGHDNSSFVVPQQAKLSDGLRVWDMSAGQSHSLLLADGDCVQPVLLYCGRQKESTSDQREKSYQRSPRKAEHYTVRPTLLPVCLEMGYISSVCSGGQSCAVLADQNIKVFISALHELASRERQFYCWLSRVRKLVLTPIQNKESTSPFLGDPCTHLFSSLCGSFSQLSVLIGRHSTSLTYFLQKAQGRDVTSLPLLTHMEQFLDTYKEYCSSVGDFQVMGGFQTLHKLTLECLQPEVLTQLCMSDQTSDDTGLVSLLYWPLQQLHQYDRVMVKVAFCFDVLMPEFQSFRQGCGQYESLSLSLLRKKKEAESTFLFWKSHSGKTTESLRLPRRRVVCESSSRSLTLQKAGRFSNHWFILFNDALVHTQFSTHHVYPLTCLWVKPVTDDSSGLYAIKIICPEESFTMVASTPQEKNKWLRSINHALDQVLGGSGEGSSPGTMGMSRTASYTFTGEGRFKDAKYSGSWMSGRVQGRGSLRWPDGRTYCGNFKNGLEDGYGECVIPNKELNKPDSYHGHWREGKIHGFGKYKYASGEVYEGCFSEGQRHGYGMLSSGKLGKKSSSVFIGHWVHDKKTGYGVFDDITRGEKYMGLWVDEKRHGSGVVVTQYGVYYEGTFRDNKMSGPGLLMSDDDTAIHGEFSDDWVVNGKGVLLLANGDCLEGLFSGEWTSGLKVDGTYTKPLVDEPVNKERKNMFKLGRYVVPAGQRWVCVFEECWSRLGCDSTGRGDKNVAWENIAVSITAARRQSPDLSRSQNKVLESLEFIPQHEQPVTTSNYDSIRRYLIKACETPLHPLGWLVETLVTVYRMTYVGVEANRRLLEQAVQELQAYLTHLYNIVQFLFPGMPDDGAVIPDPPGSPSVVQNSSSMEDLSAVVVSCSSLLLPLLLPRLYPPLFTLYRLQEEQEDALYWDRILRLNKQPDQSLLSFLGVQEKFWPTWMSILGEKKQIVSSSKDACFVSAVETLQQISTTFTPSDKLVVIQKTFEELTQEVNPMLDGHFLWCMDDLLPLFIYVVVRARIRNLGAEVDMIEDLMDPNVQHGELGLMFTTLKACYIQIKNESTT

Target ORF information:

RefSeq Version	XM_011611399.2
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes alsin-like (LOC101078293), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011611399.2

ORF Insert Sequence:

ATGGAGAACG CAGAGGACAG CCCAGCAGAT GAGTTGGTCT CCAGAGCTCC AGAGAGTGGA 
CTTTTCCACA TCTGGCAGTC AGCAGGCCCC AGTGCCCAGC TGTGTCCGGA GAGAGTGCTG 
CTGTCTATTC CTGTTGTGCA GGCTGCCCTG GGAGAGCATC ATGGAGTCCT CTTGGTGCAG 
GGGGGCCTCG TCTATTCATT TGGAGAGCTC TTGTGGAAAG ATCTGAGTGT CCCCGCATCT 
GCTCCAGTCT TGGAAGGCTC TCTGCTGGGA CGGGTGGTGG TCCGGGTGGC TGCAGGCGGT 
TTCCACTGTG GCGCTGTGAG CGAACAGGGA CATGTGTACA CATGGGGGGA GAACACTGGA 
GGACAGTGTG GGCTGACAGA GCGGACTACA GAAAAAACCA TCACAGTTTC AGAGCCTTCT 
CTTGTTAGCG TGGTCGACCA TGAGGTCATC CCGCCAGCGG CTGTTAGGGT TGTGGATCTT 
GCTTTTGGAC GCGAGCACAG TCTGGCTCTG TCAGCCCAGA ACGAGCTGTG GGCGTGGGGC 
AGCGGCTGCC AGCTTGGCCT GGTCACCACC ACCTTCCCTG TGTGTAGACC ACAGAAGGTG 
GAGCATTTAG CAGGCAGACA TGTTATTCAG GTTGTTTGCG GTGCCTACCA CAGCTTGGCC 
CTGGTTCGTA GTTTGCCTCC AAATAGCTAC AACAGCCAGA AGCCTTCTGA AAAAAGGGAA 
CGGGGTCACT CGCCGCACTA TTCAGCAGCA GAGAGAGAGG AGCTGTTTGT GGGTGACAGC 
AGTCTCTACT GTCCACTTGG GGTGGAGCTG AAAGAAGGCA TGGCCACAGA GACTTCCTCC 
AGGAGATGTC CAAGGAGAAG GCTCCATCCT GGAGGAAGTT CGGCCAGCAG CAGCCCTGGC 
TCTGCATTTA ACGAAGACGC GACCAAAGAC ACTGACTCAC TGAGCAGCTG CACCTCAGAT 
GACAGCTGTG TTTCCTCAAC TCTCTCCACT GATATGCTGA CCTCCAGTTA CAAAGAACCT 
AACAATAACT TTGGAGTGAC CCTGCCGTGC TCGTCCTCCT CACTGTGTCT CGACGATGTC 
CGACTCTGTC TTGAATCGGG GCTGCAGGTA CAGCCAGGGA AGAAGCTCTC CAGCCTCTCC 
AATAAACACT ATAAAGCAAA ATCAGCAGGA ACCAGAAGAC ATTCTGTCCC TGGGACACCC 
ACTCGTGGGT CTCCACGGCG ACAGCGTGCA AACGCGTGCG GAAAATCTTC TGGTGGTCAG 
ACCCAGGCGG GAGCGCCGGA GGAGGATGAT GATGATGGGC TGCCATCTCT AGACACAGAA 
GTCTGGAGCT GGGGACGGGG CTCTGAGGGG CAGCTCGGCC ACGGAGATCA GCTCGCCAGA 
CTTCAGCCTC TGTGTATCAA GACACTGTCG GGTGAGGAGG TGATCCAAGT TGCAGCAGGG 
TCCCATCATT CTCTGGCTGT CACTGCAACC TGTCAGGTGT ACTCATGGGG CAGCAACATG 
TGTGGGCAGC TGGGTCACGA TAACAGTTCT TTCGTTGTGC CCCAACAGGC AAAGCTGTCT 
GATGGCCTCC GAGTTTGGGA CATGTCTGCC GGCCAGAGCC ACTCCCTCCT CCTGGCTGAT 
GGAGACTGCG TCCAGCCCGT GTTGTTGTAC TGTGGCCGGC AGAAAGAGTC GACATCAGAC 
CAGAGAGAAA AGTCCTACCA GAGGTCCCCC AGAAAAGCAG AGCATTACAC AGTCAGGCCC 
ACCCTGCTGC CTGTCTGCCT GGAGATGGGC TACATCAGCA GTGTGTGCAG CGGCGGGCAA 
AGCTGCGCCG TCCTGGCAGA CCAGAACATC AAGGTTTTTA TTTCGGCCCT CCACGAGCTG 
GCCTCCAGAG AGAGGCAGTT CTACTGCTGG TTGAGTCGCG TCAGGAAACT CGTCCTCACA 
CCAATACAGA ACAAAGAGAG TACGTCTCCG TTTTTAGGTG ACCCTTGCAC CCATCTCTTT 
TCTTCTCTCT GTGGGAGTTT TTCTCAGCTA AGCGTCCTAA TCGGTCGACA CTCCACATCG 
CTTACCTACT TCCTGCAAAA GGCACAGGGC CGTGACGTCA CCTCACTCCC CCTGCTCACA 
CACATGGAGC AGTTTCTGGA CACTTACAAA GAATATTGTT CCTCTGTTGG TGACTTTCAG 
GTGATGGGGG GCTTCCAGAC CCTCCATAAG CTCACACTTG AGTGTTTGCA GCCAGAAGTT 
CTCACTCAGC TGTGTATGTC GGACCAGACC AGTGATGACA CTGGTCTGGT TTCGCTGTTG 
TACTGGCCTC TGCAGCAGCT CCACCAGTAC GACCGAGTCA TGGTCAAAGT GGCATTTTGC 
TTCGACGTGT TAATGCCAGA GTTTCAGTCC TTCCGTCAGG GCTGCGGTCA GTACGAGTCC 
CTGTCTCTGT CCCTGTTGAG GAAGAAAAAA GAGGCTGAAA GCACATTCCT CTTCTGGAAG 
AGCCACTCCG GAAAAACTAC TGAGAGTCTA CGTCTCCCTC GGCGCCGAGT GGTGTGTGAA 
AGCAGCAGCA GATCTTTGAC TCTGCAAAAG GCCGGGCGCT TCTCCAACCA CTGGTTCATT 
CTGTTCAATG ATGCACTGGT CCACACGCAG TTCTCCACAC ATCACGTCTA CCCCCTGACC 
TGCCTGTGGG TGAAACCCGT CACTGACGAC AGCAGCGGGC TCTATGCCAT TAAAATTATT 
TGCCCGGAGG AAAGTTTCAC CATGGTGGCT TCAACACCTC AAGAAAAGAA CAAGTGGTTA 
CGATCTATCA ATCACGCGCT GGATCAGGTG CTCGGTGGTA GTGGTGAGGG GTCGTCGCCG 
GGGACGATGG GAATGTCCCG CACAGCCTCC TACACATTCA CCGGAGAAGG CCGGTTTAAA 
GATGCCAAAT ACAGCGGCAG CTGGATGTCA GGACGGGTGC AGGGCAGAGG GTCCTTGAGG 
TGGCCTGATG GGCGGACCTA CTGCGGGAAC TTCAAGAACG GCCTTGAAGA TGGTTACGGG 
GAGTGCGTAA TACCCAACAA AGAGCTGAAC AAGCCTGACA GTTACCATGG ACACTGGAGA 
GAGGGGAAGA TCCACGGTTT CGGCAAATAC AAGTACGCCA GCGGCGAGGT GTATGAAGGC 
TGTTTCAGTG AAGGGCAGCG GCACGGTTAC GGCATGCTGA GCTCCGGGAA GCTGGGGAAG 
AAGTCCTCCA GTGTTTTCAT CGGCCACTGG GTCCATGACA AGAAGACAGG TTATGGAGTG 
TTTGACGACA TCACCAGGGG TGAGAAGTAC ATGGGATTGT GGGTAGACGA GAAGCGGCAC 
GGCAGCGGCG TGGTGGTCAC ACAGTATGGC GTGTACTACG AAGGAACCTT CAGAGACAAC 
AAGATGAGTG GTCCCGGTCT GCTGATGTCG GATGACGACA CGGCTATTCA TGGGGAGTTT 
TCCGACGATT GGGTTGTCAA CGGGAAGGGT GTTTTATTGT TGGCCAACGG AGACTGTCTG 
GAGGGTCTGT TCAGTGGGGA GTGGACATCT GGACTGAAGG TGGATGGAAC GTACACCAAA 
CCACTGGTGG ACGAGCCTGT AAACAAAGAA AGAAAAAACA TGTTCAAGCT GGGTAGGTAC 
GTGGTGCCTG CGGGTCAGAG GTGGGTCTGT GTGTTTGAGG AGTGCTGGAG TCGACTGGGC 
TGCGACAGCA CTGGGAGAGG AGACAAAAAT GTAGCCTGGG AGAACATCGC AGTGTCCATA 
ACGGCTGCAC GGAGGCAAAG TCCAGATCTC AGTCGGTCTC AGAACAAAGT GTTGGAGTCT 
CTGGAGTTTA TCCCTCAGCA TGAGCAGCCA GTCACCACCT CGAACTACGA CAGCATACGC 
AGATACCTCA TCAAGGCTTG TGAGACCCCC CTCCACCCTC TGGGCTGGCT GGTGGAGACT 
CTGGTGACGG TGTACCGGAT GACGTATGTG GGCGTGGAAG CTAACCGGAG GCTGCTGGAA 
CAGGCTGTCC AGGAGCTTCA GGCCTACCTC ACACATCTCT ACAACATTGT CCAGTTTTTG 
TTTCCAGGGA TGCCAGATGA TGGCGCCGTC ATCCCAGACC CCCCGGGGTC TCCATCGGTA 
GTCCAGAACA GCTCCAGCAT GGAAGACTTG AGTGCTGTAG TGGTGAGCTG CTCCTCTCTG 
CTCCTCCCTC TGCTCCTGCC TCGACTCTAC CCTCCTCTCT TCACGCTGTA CCGGCTGCAG 
GAGGAGCAGG AAGACGCACT GTACTGGGAC CGCATCCTGC GTCTGAACAA ACAACCCGAT 
CAGTCGCTGC TGAGCTTCCT GGGAGTGCAA GAGAAGTTCT GGCCTACGTG GATGTCAATT 
TTGGGGGAGA AGAAGCAGAT TGTGTCGAGC AGCAAAGACG CCTGCTTCGT TTCCGCAGTT 
GAGACCCTCC AACAAATCAG CACCACCTTT ACTCCATCTG ACAAACTCGT CGTCATCCAG 
AAGACCTTTG AGGAGTTGAC CCAGGAAGTC AATCCCATGC TGGACGGCCA CTTCCTGTGG 
TGCATGGATG ACTTGCTCCC ACTCTTCATC TACGTGGTGG TCAGAGCGAG GATCAGGAAT 
CTGGGAGCGG AGGTCGATAT GATTGAAGAT TTGATGGATC CGAATGTTCA GCATGGAGAG 
CTGGGACTGA TGTTCACCAC CCTGAAGGCC TGCTACATCC AGATCAAAAA CGAGTCTACG 
ACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101078293 ( XM_011611395.1 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_011609697.1 Takifugu rubripes alsin-like (LOC101078293), transcript variant X1, mRNA.

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CloneID	OTo30993
Clone ID Related Accession (Same CDS sequence)	XM_011611395.1
Accession Version	XM_011611395.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4710bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-15
Organism	Takifugu rubripes(Fugu rubripes)
Product	alsin-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004070909.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Takifugu rubripes Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAACG CAGAGGACAG CCCAGCAGAT GAGTTGGTCT CCAGAGCTCC AGAGAGTGGA 
CTTTTCCACA TCTGGCAGTC AGCAGGCCCC AGTGCCCAGC TGTGTCCGGA GAGAGTGCTG 
CTGTCTATTC CTGTTGTGCA GGCTGCCCTG GGAGAGCATC ATGGAGTCCT CTTGGTGCAG 
GGGGGCCTCG TCTATTCATT TGGAGAGCTC TTGTGGAAAG ATCTGAGTGT CCCCGCATCT 
GCTCCAGTCT TGGAAGGCTC TCTGCTGGGA CGGGTGGTGG TCCGGGTGGC TGCAGGCGGT 
TTCCACTGTG GCGCTGTGAG CGAACAGGGA CATGTGTACA CATGGGGGGA GAACACTGGA 
GGACAGTGTG GGCTGACAGA GCGGACTACA GAAAAAACCA TCACAGTTTC AGAGCCTTCT 
CTTGTTAGCG TGGTCGACCA TGAGGTCATC CCGCCAGCGG CTGTTAGGGT TGTGGATCTT 
GCTTTTGGAC GCGAGCACAG TCTGGCTCTG TCAGCCCAGA ACGAGCTGTG GGCGTGGGGC 
AGCGGCTGCC AGCTTGGCCT GGTCACCACC ACCTTCCCTG TGTGTAGACC ACAGAAGGTG 
GAGCATTTAG CAGGCAGACA TGTTATTCAG GTTGTTTGCG GTGCCTACCA CAGCTTGGCC 
CTGGTTCGTA GTTTGCCTCC AAATAGCTAC AACAGCCAGA AGCCTTCTGA AAAAAGGGAA 
CGGGGTCACT CGCCGCACTA TTCAGCAGCA GAGAGAGAGG AGCTGTTTGT GGGTGACAGC 
AGTCTCTACT GTCCACTTGG GGTGGAGCTG AAAGAAGGCA TGGCCACAGA GACTTCCTCC 
AGGAGATGTC CAAGGAGAAG GCTCCATCCT GGAGGAAGTT CGGCCAGCAG CAGCCCTGGC 
TCTGCATTTA ACGAAGACGC GACCAAAGAC ACTGACTCAC TGAGCAGCTG CACCTCAGAT 
GACAGCTGTG TTTCCTCAAC TCTCTCCACT GATATGCTGA CCTCCAGTTA CAAAGAACCT 
AACAATAACT TTGGAGTGAC CCTGCCGTGC TCGTCCTCCT CACTGTGTCT CGACGATGTC 
CGACTCTGTC TTGAATCGGG GCTGCAGGTA CAGCCAGGGA AGAAGCTCTC CAGCCTCTCC 
AATAAACACT ATAAAGCAAA ATCAGCAGGA ACCAGAAGAC ATTCTGTCCC TGGGACACCC 
ACTCGTGGGT CTCCACGGCG ACAGCGTGCA AACGCGTGCG GAAAATCTTC TGGTGGTCAG 
ACCCAGGCGG GAGCGCCGGA GGAGGATGAT GATGATGGGC TGCCATCTCT AGACACAGAA 
GTCTGGAGCT GGGGACGGGG CTCTGAGGGG CAGCTCGGCC ACGGAGATCA GCTCGCCAGA 
CTTCAGCCTC TGTGTATCAA GACACTGTCG GGTGAGGAGG TGATCCAAGT TGCAGCAGGG 
TCCCATCATT CTCTGGCTGT CACTGCAACC TGTCAGGTGT ACTCATGGGG CAGCAACATG 
TGTGGGCAGC TGGGTCACGA TAACAGTTCT TTCGTTGTGC CCCAACAGGC AAAGCTGTCT 
GATGGCCTCC GAGTTTGGGA CATGTCTGCC GGCCAGAGCC ACTCCCTCCT CCTGGCTGAT 
GGAGACTGCG TCCAGCCCGT GTTGTTGTAC TGTGGCCGGC AGAAAGAGTC GACATCAGAC 
CAGAGAGAAA AGTCCTACCA GAGGTCCCCC AGAAAAGCAG AGCATTACAC AGTCAGGCCC 
ACCCTGCTGC CTGTCTGCCT GGAGATGGGC TACATCAGCA GTGTGTGCAG CGGCGGGCAA 
AGCTGCGCCG TCCTGGCAGA CCAGAACATC AAGGTTTTTA TTTCGGCCCT CCACGAGCTG 
GCCTCCAGAG AGAGGCAGTT CTACTGCTGG TTGAGTCGCG TCAGGAAACT CGTCCTCACA 
CCAATACAGA ACAAAGAGAG TACGTCTCCG TTTTTAGGTG ACCCTTGCAC CCATCTCTTT 
TCTTCTCTCT GTGGGAGTTT TTCTCAGCTA AGCGTCCTAA TCGGTCGACA CTCCACATCG 
CTTACCTACT TCCTGCAAAA GGCACAGGGC CGTGACGTCA CCTCACTCCC CCTGCTCACA 
CACATGGAGC AGTTTCTGGA CACTTACAAA GAATATTGTT CCTCTGTTGG TGACTTTCAG 
GTGATGGGGG GCTTCCAGAC CCTCCATAAG CTCACACTTG AGTGTTTGCA GCCAGAAGTT 
CTCACTCAGC TGTGTATGTC GGACCAGACC AGTGATGACA CTGGTCTGGT TTCGCTGTTG 
TACTGGCCTC TGCAGCAGCT CCACCAGTAC GACCGAGTCA TGGTCAAAGT GGCGTTTTGC 
TTCGACGTGT TAATGCCAGA GTTTCAGTCC TTCCGTCAGG GCTGCGGTCA GTACGAGTCC 
CTGTCTCTGT CCCTGTTGAG GAAGAAAAAA GAGGCTGAAA GCACATTCCT CTTCTGGAAG 
AGCCACTCCG GAAAAACTAC TGAGAGTCTA CGTCTCCCTC GGCGCCGAGT GGTGTGTGAA 
AGCAGCAGCA GATCTTTGAC TCTGCAAAAG GCCGGGCGCT TCTCCAACCA CTGGTTCATT 
CTGTTCAATG ATGCACTGGT CCACACGCAG GGCGCGCTTC CCTCACAGCT CTTTTTCTCC 
ACACATCACG TCTACCCCCT GACCTGCCTG TGGGTGAAAC CCGTCACTGA CGACAGCAGC 
GGACTCTATG CCATTAAAAT TATTTGCCCG GAGGAAAGTT TCACCATGGT GGCTTCAACA 
CCTCAAGAAA AGAACAAGTG GTTACGATCT ATCAATCACG CGCTGGATCA GGTGCTCGGT 
GGTAGTGGTG AGGGGTCGTC GCCGGGGACG ATGGGAATGT CCCGCACGGC CTCCTACACA 
TTCACCGGAG AAGGCCGGTT TAAAGATGCC AAATACAGCG GCAGCTGGAT GTCAGGACGG 
GTGCATGGCA GAGGGTCCTT GAGGTGGCCT GATGGGCGGA CCTACTGCGG GAACTTCAAG 
AACGGCCTTG AAGATGGTTA CGGGGAGTGC GTAATACCCA ACAAAGAGCT GAACAAGCCT 
GACAGTTACC ATGGACACTG GAGAGAGGGG AAGATCCACG GTTTCGGCAA ATACAAGTAC 
GCCAGCGGCG AGGTGTATGA AGGCTGTTTC AGTGAAGGGC AGCGGCAGGG TTACGGCATG 
CTGAGCTCCG GGAAGCTGGG GAAGAAGTCC TCCAGTGTTT TCATCGGCCA CTGGGTCCAT 
GACAAGAAGA CAGGTTATGG AGTGTTTGAC GACATCACCA GGGGTGAGAA GTACATGGGA 
TTGTGGGTAG ACGAGAAGCG GCACGGCAGC GGCGTGGTGG TCACACAGTA TGGCGTGTAC 
TACGAAGGAA CCTTCAGAGA CAACAAGATG AGTGGTCCCG GTCTGCTGAT GTCGGATGAC 
GACACGGCTA TTCATGGGGA GTTTTCCGAC GATTGGGTTG TCAACGGGAA GGGTGTTTTA 
TTGTTGGCCA ACGGAGACTG TCTGGAGGGT CTGTTCAGTG GGGAGTGGAC ATCTGGACTG 
AAGGTGGATG GAACGTACAC CAAACCACTG GTGGACGAGC CTGTAAACAA AGAAAGAAAA 
AACATGTTCA AGCTGGGTAG GTACGTGGTG CCTGCGGGTC AGAGGTGGGT CTGTGTGTTT 
GAGGAGTGCT GGAGTCGACT GGGCTGCGAC AGCACTGGGA GAGGAGACAA AAATGTAGCC 
TGGGAGAACA TCGCAGTGTC CATAACGGCT GCACGGAGGC AAAGTCCAGA TCTCAGTCGG 
TCTCAGAACA AAGTGTTGGA GTCTCTGGAG TTTATCCCTC AGCATGAGCA GCCAGTCACC 
ACCTCGAACT ACGACAGCAT ACGCAGATAC CTCATCAAGG CTTGTGAGAC CCCCCTCCAC 
CCTCTGGGCT GGCTGGTGGA GACTCTGGTG ACGGTGTACC GGATGACGTA TGTGGGCGTG 
GAAGCTAACC GGAGGCTGCT GGAACAGGCT GTCCAGGAGC TTCAGGCCTA CCTCACACAT 
CTCTACAACA TTGTCCAGTT TTTGTTTCCA GGGATGCCAG ATGATGGCGC CGTCATCCCA 
GACCCCCCGG GGTCTCCATC GGTAGTCCAG AACAGCTCCA GCATGGAAGA CTTGAGTGCT 
GTAGTGGTGA GCTGCTCCTC TCTGCTCCTC CCTCTGCTCC TGCCTCGACT CTACCCTCCT 
CTCTTCACGC TGTACCGGCT GCAGGAGGAG CAGGAAGACG CACTGTACTG GGACCGCATC 
CTGCGTCTGA ACAAACAACC CGATCAGTCG CTGCTGAGCT TCCTGGGAGT GCAAGAGAAG 
TTCTGGCCTA CGTGGATGTC AATTTTGGGG GAGAAGAAGC AGATTGTGTC GAGCAGCAAA 
GACGCCTGCT TCGTTTCCGC AGTTGAGACC CTCCAACAAA TCAGCACCAC CTTTACTCCA 
TCTGACAAAC TCGTCGTCAT CCAGAAGACC TTTGAGGAGT TGACCCAGGA AGTCAATCCC 
ATGCTGGACG GCCACTTCCT GTGGTGCATG GATGACTTGC TCCCACTCTT CATCTACGTG 
GTGGTCAGAG CGAGGATCAG GAATCTGGGA GCGGAGGTCG ATATGATTGA AGATTTGATG 
GATCCGAATG TTCAGCATGG AGAGCTGGGA CTGATGTTCA CCACCCTGAA GGCCTGCTAC 
ATCCAGATCA AAAACGAGTC TACGACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011609697.1
CDS	231..4940
Translation	MENAEDSPADELVSRAPESGLFHIWQSAGPSAQLCPERVLLSIPVVQAALGEHHGVLLVQGGLVYSFGELLWKDLSVPASAPVLEGSLLGRVVVRVAAGGFHCGAVSEQGHVYTWGENTGGQCGLTERTTEKTITVSEPSLVSVVDHEVIPPAAVRVVDLAFGREHSLALSAQNELWAWGSGCQLGLVTTTFPVCRPQKVEHLAGRHVIQVVCGAYHSLALVRSLPPNSYNSQKPSEKRERGHSPHYSAAEREELFVGDSSLYCPLGVELKEGMATETSSRRCPRRRLHPGGSSASSSPGSAFNEDATKDTDSLSSCTSDDSCVSSTLSTDMLTSSYKEPNNNFGVTLPCSSSSLCLDDVRLCLESGLQVQPGKKLSSLSNKHYKAKSAGTRRHSVPGTPTRGSPRRQRANACGKSSGGQTQAGAPEEDDDDGLPSLDTEVWSWGRGSEGQLGHGDQLARLQPLCIKTLSGEEVIQVAAGSHHSLAVTATCQVYSWGSNMCGQLGHDNSSFVVPQQAKLSDGLRVWDMSAGQSHSLLLADGDCVQPVLLYCGRQKESTSDQREKSYQRSPRKAEHYTVRPTLLPVCLEMGYISSVCSGGQSCAVLADQNIKVFISALHELASRERQFYCWLSRVRKLVLTPIQNKESTSPFLGDPCTHLFSSLCGSFSQLSVLIGRHSTSLTYFLQKAQGRDVTSLPLLTHMEQFLDTYKEYCSSVGDFQVMGGFQTLHKLTLECLQPEVLTQLCMSDQTSDDTGLVSLLYWPLQQLHQYDRVMVKVAFCFDVLMPEFQSFRQGCGQYESLSLSLLRKKKEAESTFLFWKSHSGKTTESLRLPRRRVVCESSSRSLTLQKAGRFSNHWFILFNDALVHTQGALPSQLFFSTHHVYPLTCLWVKPVTDDSSGLYAIKIICPEESFTMVASTPQEKNKWLRSINHALDQVLGGSGEGSSPGTMGMSRTASYTFTGEGRFKDAKYSGSWMSGRVHGRGSLRWPDGRTYCGNFKNGLEDGYGECVIPNKELNKPDSYHGHWREGKIHGFGKYKYASGEVYEGCFSEGQRQGYGMLSSGKLGKKSSSVFIGHWVHDKKTGYGVFDDITRGEKYMGLWVDEKRHGSGVVVTQYGVYYEGTFRDNKMSGPGLLMSDDDTAIHGEFSDDWVVNGKGVLLLANGDCLEGLFSGEWTSGLKVDGTYTKPLVDEPVNKERKNMFKLGRYVVPAGQRWVCVFEECWSRLGCDSTGRGDKNVAWENIAVSITAARRQSPDLSRSQNKVLESLEFIPQHEQPVTTSNYDSIRRYLIKACETPLHPLGWLVETLVTVYRMTYVGVEANRRLLEQAVQELQAYLTHLYNIVQFLFPGMPDDGAVIPDPPGSPSVVQNSSSMEDLSAVVVSCSSLLLPLLLPRLYPPLFTLYRLQEEQEDALYWDRILRLNKQPDQSLLSFLGVQEKFWPTWMSILGEKKQIVSSSKDACFVSAVETLQQISTTFTPSDKLVVIQKTFEELTQEVNPMLDGHFLWCMDDLLPLFIYVVVRARIRNLGAEVDMIEDLMDPNVQHGELGLMFTTLKACYIQIKNESTT

Target ORF information:

RefSeq Version	XM_011611395.1
Organism	Takifugu rubripes(Fugu rubripes)
Definition	Takifugu rubripes alsin-like (LOC101078293), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011611395.1

ORF Insert Sequence:

ATGGAGAACG CAGAGGACAG CCCAGCAGAT GAGTTGGTCT CCAGAGCTCC AGAGAGTGGA 
CTTTTCCACA TCTGGCAGTC AGCAGGCCCC AGTGCCCAGC TGTGTCCGGA GAGAGTGCTG 
CTGTCTATTC CTGTTGTGCA GGCTGCCCTG GGAGAGCATC ATGGAGTCCT CTTGGTGCAG 
GGGGGCCTCG TCTATTCATT TGGAGAGCTC TTGTGGAAAG ATCTGAGTGT CCCCGCATCT 
GCTCCAGTCT TGGAAGGCTC TCTGCTGGGA CGGGTGGTGG TCCGGGTGGC TGCAGGCGGT 
TTCCACTGTG GCGCTGTGAG CGAACAGGGA CATGTGTACA CATGGGGGGA GAACACTGGA 
GGACAGTGTG GGCTGACAGA GCGGACTACA GAAAAAACCA TCACAGTTTC AGAGCCTTCT 
CTTGTTAGCG TGGTCGACCA TGAGGTCATC CCGCCAGCGG CTGTTAGGGT TGTGGATCTT 
GCTTTTGGAC GCGAGCACAG TCTGGCTCTG TCAGCCCAGA ACGAGCTGTG GGCGTGGGGC 
AGCGGCTGCC AGCTTGGCCT GGTCACCACC ACCTTCCCTG TGTGTAGACC ACAGAAGGTG 
GAGCATTTAG CAGGCAGACA TGTTATTCAG GTTGTTTGCG GTGCCTACCA CAGCTTGGCC 
CTGGTTCGTA GTTTGCCTCC AAATAGCTAC AACAGCCAGA AGCCTTCTGA AAAAAGGGAA 
CGGGGTCACT CGCCGCACTA TTCAGCAGCA GAGAGAGAGG AGCTGTTTGT GGGTGACAGC 
AGTCTCTACT GTCCACTTGG GGTGGAGCTG AAAGAAGGCA TGGCCACAGA GACTTCCTCC 
AGGAGATGTC CAAGGAGAAG GCTCCATCCT GGAGGAAGTT CGGCCAGCAG CAGCCCTGGC 
TCTGCATTTA ACGAAGACGC GACCAAAGAC ACTGACTCAC TGAGCAGCTG CACCTCAGAT 
GACAGCTGTG TTTCCTCAAC TCTCTCCACT GATATGCTGA CCTCCAGTTA CAAAGAACCT 
AACAATAACT TTGGAGTGAC CCTGCCGTGC TCGTCCTCCT CACTGTGTCT CGACGATGTC 
CGACTCTGTC TTGAATCGGG GCTGCAGGTA CAGCCAGGGA AGAAGCTCTC CAGCCTCTCC 
AATAAACACT ATAAAGCAAA ATCAGCAGGA ACCAGAAGAC ATTCTGTCCC TGGGACACCC 
ACTCGTGGGT CTCCACGGCG ACAGCGTGCA AACGCGTGCG GAAAATCTTC TGGTGGTCAG 
ACCCAGGCGG GAGCGCCGGA GGAGGATGAT GATGATGGGC TGCCATCTCT AGACACAGAA 
GTCTGGAGCT GGGGACGGGG CTCTGAGGGG CAGCTCGGCC ACGGAGATCA GCTCGCCAGA 
CTTCAGCCTC TGTGTATCAA GACACTGTCG GGTGAGGAGG TGATCCAAGT TGCAGCAGGG 
TCCCATCATT CTCTGGCTGT CACTGCAACC TGTCAGGTGT ACTCATGGGG CAGCAACATG 
TGTGGGCAGC TGGGTCACGA TAACAGTTCT TTCGTTGTGC CCCAACAGGC AAAGCTGTCT 
GATGGCCTCC GAGTTTGGGA CATGTCTGCC GGCCAGAGCC ACTCCCTCCT CCTGGCTGAT 
GGAGACTGCG TCCAGCCCGT GTTGTTGTAC TGTGGCCGGC AGAAAGAGTC GACATCAGAC 
CAGAGAGAAA AGTCCTACCA GAGGTCCCCC AGAAAAGCAG AGCATTACAC AGTCAGGCCC 
ACCCTGCTGC CTGTCTGCCT GGAGATGGGC TACATCAGCA GTGTGTGCAG CGGCGGGCAA 
AGCTGCGCCG TCCTGGCAGA CCAGAACATC AAGGTTTTTA TTTCGGCCCT CCACGAGCTG 
GCCTCCAGAG AGAGGCAGTT CTACTGCTGG TTGAGTCGCG TCAGGAAACT CGTCCTCACA 
CCAATACAGA ACAAAGAGAG TACGTCTCCG TTTTTAGGTG ACCCTTGCAC CCATCTCTTT 
TCTTCTCTCT GTGGGAGTTT TTCTCAGCTA AGCGTCCTAA TCGGTCGACA CTCCACATCG 
CTTACCTACT TCCTGCAAAA GGCACAGGGC CGTGACGTCA CCTCACTCCC CCTGCTCACA 
CACATGGAGC AGTTTCTGGA CACTTACAAA GAATATTGTT CCTCTGTTGG TGACTTTCAG 
GTGATGGGGG GCTTCCAGAC CCTCCATAAG CTCACACTTG AGTGTTTGCA GCCAGAAGTT 
CTCACTCAGC TGTGTATGTC GGACCAGACC AGTGATGACA CTGGTCTGGT TTCGCTGTTG 
TACTGGCCTC TGCAGCAGCT CCACCAGTAC GACCGAGTCA TGGTCAAAGT GGCGTTTTGC 
TTCGACGTGT TAATGCCAGA GTTTCAGTCC TTCCGTCAGG GCTGCGGTCA GTACGAGTCC 
CTGTCTCTGT CCCTGTTGAG GAAGAAAAAA GAGGCTGAAA GCACATTCCT CTTCTGGAAG 
AGCCACTCCG GAAAAACTAC TGAGAGTCTA CGTCTCCCTC GGCGCCGAGT GGTGTGTGAA 
AGCAGCAGCA GATCTTTGAC TCTGCAAAAG GCCGGGCGCT TCTCCAACCA CTGGTTCATT 
CTGTTCAATG ATGCACTGGT CCACACGCAG GGCGCGCTTC CCTCACAGCT CTTTTTCTCC 
ACACATCACG TCTACCCCCT GACCTGCCTG TGGGTGAAAC CCGTCACTGA CGACAGCAGC 
GGACTCTATG CCATTAAAAT TATTTGCCCG GAGGAAAGTT TCACCATGGT GGCTTCAACA 
CCTCAAGAAA AGAACAAGTG GTTACGATCT ATCAATCACG CGCTGGATCA GGTGCTCGGT 
GGTAGTGGTG AGGGGTCGTC GCCGGGGACG ATGGGAATGT CCCGCACGGC CTCCTACACA 
TTCACCGGAG AAGGCCGGTT TAAAGATGCC AAATACAGCG GCAGCTGGAT GTCAGGACGG 
GTGCATGGCA GAGGGTCCTT GAGGTGGCCT GATGGGCGGA CCTACTGCGG GAACTTCAAG 
AACGGCCTTG AAGATGGTTA CGGGGAGTGC GTAATACCCA ACAAAGAGCT GAACAAGCCT 
GACAGTTACC ATGGACACTG GAGAGAGGGG AAGATCCACG GTTTCGGCAA ATACAAGTAC 
GCCAGCGGCG AGGTGTATGA AGGCTGTTTC AGTGAAGGGC AGCGGCAGGG TTACGGCATG 
CTGAGCTCCG GGAAGCTGGG GAAGAAGTCC TCCAGTGTTT TCATCGGCCA CTGGGTCCAT 
GACAAGAAGA CAGGTTATGG AGTGTTTGAC GACATCACCA GGGGTGAGAA GTACATGGGA 
TTGTGGGTAG ACGAGAAGCG GCACGGCAGC GGCGTGGTGG TCACACAGTA TGGCGTGTAC 
TACGAAGGAA CCTTCAGAGA CAACAAGATG AGTGGTCCCG GTCTGCTGAT GTCGGATGAC 
GACACGGCTA TTCATGGGGA GTTTTCCGAC GATTGGGTTG TCAACGGGAA GGGTGTTTTA 
TTGTTGGCCA ACGGAGACTG TCTGGAGGGT CTGTTCAGTG GGGAGTGGAC ATCTGGACTG 
AAGGTGGATG GAACGTACAC CAAACCACTG GTGGACGAGC CTGTAAACAA AGAAAGAAAA 
AACATGTTCA AGCTGGGTAG GTACGTGGTG CCTGCGGGTC AGAGGTGGGT CTGTGTGTTT 
GAGGAGTGCT GGAGTCGACT GGGCTGCGAC AGCACTGGGA GAGGAGACAA AAATGTAGCC 
TGGGAGAACA TCGCAGTGTC CATAACGGCT GCACGGAGGC AAAGTCCAGA TCTCAGTCGG 
TCTCAGAACA AAGTGTTGGA GTCTCTGGAG TTTATCCCTC AGCATGAGCA GCCAGTCACC 
ACCTCGAACT ACGACAGCAT ACGCAGATAC CTCATCAAGG CTTGTGAGAC CCCCCTCCAC 
CCTCTGGGCT GGCTGGTGGA GACTCTGGTG ACGGTGTACC GGATGACGTA TGTGGGCGTG 
GAAGCTAACC GGAGGCTGCT GGAACAGGCT GTCCAGGAGC TTCAGGCCTA CCTCACACAT 
CTCTACAACA TTGTCCAGTT TTTGTTTCCA GGGATGCCAG ATGATGGCGC CGTCATCCCA 
GACCCCCCGG GGTCTCCATC GGTAGTCCAG AACAGCTCCA GCATGGAAGA CTTGAGTGCT 
GTAGTGGTGA GCTGCTCCTC TCTGCTCCTC CCTCTGCTCC TGCCTCGACT CTACCCTCCT 
CTCTTCACGC TGTACCGGCT GCAGGAGGAG CAGGAAGACG CACTGTACTG GGACCGCATC 
CTGCGTCTGA ACAAACAACC CGATCAGTCG CTGCTGAGCT TCCTGGGAGT GCAAGAGAAG 
TTCTGGCCTA CGTGGATGTC AATTTTGGGG GAGAAGAAGC AGATTGTGTC GAGCAGCAAA 
GACGCCTGCT TCGTTTCCGC AGTTGAGACC CTCCAACAAA TCAGCACCAC CTTTACTCCA 
TCTGACAAAC TCGTCGTCAT CCAGAAGACC TTTGAGGAGT TGACCCAGGA AGTCAATCCC 
ATGCTGGACG GCCACTTCCT GTGGTGCATG GATGACTTGC TCCCACTCTT CATCTACGTG 
GTGGTCAGAG CGAGGATCAG GAATCTGGGA GCGGAGGTCG ATATGATTGA AGATTTGATG 
GATCCGAATG TTCAGCATGG AGAGCTGGGA CTGATGTTCA CCACCCTGAA GGCCTGCTAC 
ATCCAGATCA AAAACGAGTC TACGACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101078293 ( XM_011611399.1 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_011609701.1 Takifugu rubripes alsin-like (LOC101078293), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OTo30994
Clone ID Related Accession (Same CDS sequence)	XM_011611399.1
Accession Version	XM_011611399.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4686bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-15
Organism	Takifugu rubripes(Fugu rubripes)
Product	alsin-like isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004070909.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Takifugu rubripes Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAACG CAGAGGACAG CCCAGCAGAT GAGTTGGTCT CCAGAGCTCC AGAGAGTGGA 
CTTTTCCACA TCTGGCAGTC AGCAGGCCCC AGTGCCCAGC TGTGTCCGGA GAGAGTGCTG 
CTGTCTATTC CTGTTGTGCA GGCTGCCCTG GGAGAGCATC ATGGAGTCCT CTTGGTGCAG 
GGGGGCCTCG TCTATTCATT TGGAGAGCTC TTGTGGAAAG ATCTGAGTGT CCCCGCATCT 
GCTCCAGTCT TGGAAGGCTC TCTGCTGGGA CGGGTGGTGG TCCGGGTGGC TGCAGGCGGT 
TTCCACTGTG GCGCTGTGAG CGAACAGGGA CATGTGTACA CATGGGGGGA GAACACTGGA 
GGACAGTGTG GGCTGACAGA GCGGACTACA GAAAAAACCA TCACAGTTTC AGAGCCTTCT 
CTTGTTAGCG TGGTCGACCA TGAGGTCATC CCGCCAGCGG CTGTTAGGGT TGTGGATCTT 
GCTTTTGGAC GCGAGCACAG TCTGGCTCTG TCAGCCCAGA ACGAGCTGTG GGCGTGGGGC 
AGCGGCTGCC AGCTTGGCCT GGTCACCACC ACCTTCCCTG TGTGTAGACC ACAGAAGGTG 
GAGCATTTAG CAGGCAGACA TGTTATTCAG GTTGTTTGCG GTGCCTACCA CAGCTTGGCC 
CTGGTTCGTA GTTTGCCTCC AAATAGCTAC AACAGCCAGA AGCCTTCTGA AAAAAGGGAA 
CGGGGTCACT CGCCGCACTA TTCAGCAGCA GAGAGAGAGG AGCTGTTTGT GGGTGACAGC 
AGTCTCTACT GTCCACTTGG GGTGGAGCTG AAAGAAGGCA TGGCCACAGA GACTTCCTCC 
AGGAGATGTC CAAGGAGAAG GCTCCATCCT GGAGGAAGTT CGGCCAGCAG CAGCCCTGGC 
TCTGCATTTA ACGAAGACGC GACCAAAGAC ACTGACTCAC TGAGCAGCTG CACCTCAGAT 
GACAGCTGTG TTTCCTCAAC TCTCTCCACT GATATGCTGA CCTCCAGTTA CAAAGAACCT 
AACAATAACT TTGGAGTGAC CCTGCCGTGC TCGTCCTCCT CACTGTGTCT CGACGATGTC 
CGACTCTGTC TTGAATCGGG GCTGCAGGTA CAGCCAGGGA AGAAGCTCTC CAGCCTCTCC 
AATAAACACT ATAAAGCAAA ATCAGCAGGA ACCAGAAGAC ATTCTGTCCC TGGGACACCC 
ACTCGTGGGT CTCCACGGCG ACAGCGTGCA AACGCGTGCG GAAAATCTTC TGGTGGTCAG 
ACCCAGGCGG GAGCGCCGGA GGAGGATGAT GATGATGGGC TGCCATCTCT AGACACAGAA 
GTCTGGAGCT GGGGACGGGG CTCTGAGGGG CAGCTCGGCC ACGGAGATCA GCTCGCCAGA 
CTTCAGCCTC TGTGTATCAA GACACTGTCG GGTGAGGAGG TGATCCAAGT TGCAGCAGGG 
TCCCATCATT CTCTGGCTGT CACTGCAACC TGTCAGGTGT ACTCATGGGG CAGCAACATG 
TGTGGGCAGC TGGGTCACGA TAACAGTTCT TTCGTTGTGC CCCAACAGGC AAAGCTGTCT 
GATGGCCTCC GAGTTTGGGA CATGTCTGCC GGCCAGAGCC ACTCCCTCCT CCTGGCTGAT 
GGAGACTGCG TCCAGCCCGT GTTGTTGTAC TGTGGCCGGC AGAAAGAGTC GACATCAGAC 
CAGAGAGAAA AGTCCTACCA GAGGTCCCCC AGAAAAGCAG AGCATTACAC AGTCAGGCCC 
ACCCTGCTGC CTGTCTGCCT GGAGATGGGC TACATCAGCA GTGTGTGCAG CGGCGGGCAA 
AGCTGCGCCG TCCTGGCAGA CCAGAACATC AAGGTTTTTA TTTCGGCCCT CCACGAGCTG 
GCCTCCAGAG AGAGGCAGTT CTACTGCTGG TTGAGTCGCG TCAGGAAACT CGTCCTCACA 
CCAATACAGA ACAAAGAGAG TACGTCTCCG TTTTTAGGTG ACCCTTGCAC CCATCTCTTT 
TCTTCTCTCT GTGGGAGTTT TTCTCAGCTA AGCGTCCTAA TCGGTCGACA CTCCACATCG 
CTTACCTACT TCCTGCAAAA GGCACAGGGC CGTGACGTCA CCTCACTCCC CCTGCTCACA 
CACATGGAGC AGTTTCTGGA CACTTACAAA GAATATTGTT CCTCTGTTGG TGACTTTCAG 
GTGATGGGGG GCTTCCAGAC CCTCCATAAG CTCACACTTG AGTGTTTGCA GCCAGAAGTT 
CTCACTCAGC TGTGTATGTC GGACCAGACC AGTGATGACA CTGGTCTGGT TTCGCTGTTG 
TACTGGCCTC TGCAGCAGCT CCACCAGTAC GACCGAGTCA TGGTCAAAGT GGCGTTTTGC 
TTCGACGTGT TAATGCCAGA GTTTCAGTCC TTCCGTCAGG GCTGCGGTCA GTACGAGTCC 
CTGTCTCTGT CCCTGTTGAG GAAGAAAAAA GAGGCTGAAA GCACATTCCT CTTCTGGAAG 
AGCCACTCCG GAAAAACTAC TGAGAGTCTA CGTCTCCCTC GGCGCCGAGT GGTGTGTGAA 
AGCAGCAGCA GATCTTTGAC TCTGCAAAAG GCCGGGCGCT TCTCCAACCA CTGGTTCATT 
CTGTTCAATG ATGCACTGGT CCACACGCAG TTCTCCACAC ATCACGTCTA CCCCCTGACC 
TGCCTGTGGG TGAAACCCGT CACTGACGAC AGCAGCGGAC TCTATGCCAT TAAAATTATT 
TGCCCGGAGG AAAGTTTCAC CATGGTGGCT TCAACACCTC AAGAAAAGAA CAAGTGGTTA 
CGATCTATCA ATCACGCGCT GGATCAGGTG CTCGGTGGTA GTGGTGAGGG GTCGTCGCCG 
GGGACGATGG GAATGTCCCG CACGGCCTCC TACACATTCA CCGGAGAAGG CCGGTTTAAA 
GATGCCAAAT ACAGCGGCAG CTGGATGTCA GGACGGGTGC ATGGCAGAGG GTCCTTGAGG 
TGGCCTGATG GGCGGACCTA CTGCGGGAAC TTCAAGAACG GCCTTGAAGA TGGTTACGGG 
GAGTGCGTAA TACCCAACAA AGAGCTGAAC AAGCCTGACA GTTACCATGG ACACTGGAGA 
GAGGGGAAGA TCCACGGTTT CGGCAAATAC AAGTACGCCA GCGGCGAGGT GTATGAAGGC 
TGTTTCAGTG AAGGGCAGCG GCAGGGTTAC GGCATGCTGA GCTCCGGGAA GCTGGGGAAG 
AAGTCCTCCA GTGTTTTCAT CGGCCACTGG GTCCATGACA AGAAGACAGG TTATGGAGTG 
TTTGACGACA TCACCAGGGG TGAGAAGTAC ATGGGATTGT GGGTAGACGA GAAGCGGCAC 
GGCAGCGGCG TGGTGGTCAC ACAGTATGGC GTGTACTACG AAGGAACCTT CAGAGACAAC 
AAGATGAGTG GTCCCGGTCT GCTGATGTCG GATGACGACA CGGCTATTCA TGGGGAGTTT 
TCCGACGATT GGGTTGTCAA CGGGAAGGGT GTTTTATTGT TGGCCAACGG AGACTGTCTG 
GAGGGTCTGT TCAGTGGGGA GTGGACATCT GGACTGAAGG TGGATGGAAC GTACACCAAA 
CCACTGGTGG ACGAGCCTGT AAACAAAGAA AGAAAAAACA TGTTCAAGCT GGGTAGGTAC 
GTGGTGCCTG CGGGTCAGAG GTGGGTCTGT GTGTTTGAGG AGTGCTGGAG TCGACTGGGC 
TGCGACAGCA CTGGGAGAGG AGACAAAAAT GTAGCCTGGG AGAACATCGC AGTGTCCATA 
ACGGCTGCAC GGAGGCAAAG TCCAGATCTC AGTCGGTCTC AGAACAAAGT GTTGGAGTCT 
CTGGAGTTTA TCCCTCAGCA TGAGCAGCCA GTCACCACCT CGAACTACGA CAGCATACGC 
AGATACCTCA TCAAGGCTTG TGAGACCCCC CTCCACCCTC TGGGCTGGCT GGTGGAGACT 
CTGGTGACGG TGTACCGGAT GACGTATGTG GGCGTGGAAG CTAACCGGAG GCTGCTGGAA 
CAGGCTGTCC AGGAGCTTCA GGCCTACCTC ACACATCTCT ACAACATTGT CCAGTTTTTG 
TTTCCAGGGA TGCCAGATGA TGGCGCCGTC ATCCCAGACC CCCCGGGGTC TCCATCGGTA 
GTCCAGAACA GCTCCAGCAT GGAAGACTTG AGTGCTGTAG TGGTGAGCTG CTCCTCTCTG 
CTCCTCCCTC TGCTCCTGCC TCGACTCTAC CCTCCTCTCT TCACGCTGTA CCGGCTGCAG 
GAGGAGCAGG AAGACGCACT GTACTGGGAC CGCATCCTGC GTCTGAACAA ACAACCCGAT 
CAGTCGCTGC TGAGCTTCCT GGGAGTGCAA GAGAAGTTCT GGCCTACGTG GATGTCAATT 
TTGGGGGAGA AGAAGCAGAT TGTGTCGAGC AGCAAAGACG CCTGCTTCGT TTCCGCAGTT 
GAGACCCTCC AACAAATCAG CACCACCTTT ACTCCATCTG ACAAACTCGT CGTCATCCAG 
AAGACCTTTG AGGAGTTGAC CCAGGAAGTC AATCCCATGC TGGACGGCCA CTTCCTGTGG 
TGCATGGATG ACTTGCTCCC ACTCTTCATC TACGTGGTGG TCAGAGCGAG GATCAGGAAT 
CTGGGAGCGG AGGTCGATAT GATTGAAGAT TTGATGGATC CGAATGTTCA GCATGGAGAG 
CTGGGACTGA TGTTCACCAC CCTGAAGGCC TGCTACATCC AGATCAAAAA CGAGTCTACG 
ACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011609701.1
CDS	231..4916
Translation	MENAEDSPADELVSRAPESGLFHIWQSAGPSAQLCPERVLLSIPVVQAALGEHHGVLLVQGGLVYSFGELLWKDLSVPASAPVLEGSLLGRVVVRVAAGGFHCGAVSEQGHVYTWGENTGGQCGLTERTTEKTITVSEPSLVSVVDHEVIPPAAVRVVDLAFGREHSLALSAQNELWAWGSGCQLGLVTTTFPVCRPQKVEHLAGRHVIQVVCGAYHSLALVRSLPPNSYNSQKPSEKRERGHSPHYSAAEREELFVGDSSLYCPLGVELKEGMATETSSRRCPRRRLHPGGSSASSSPGSAFNEDATKDTDSLSSCTSDDSCVSSTLSTDMLTSSYKEPNNNFGVTLPCSSSSLCLDDVRLCLESGLQVQPGKKLSSLSNKHYKAKSAGTRRHSVPGTPTRGSPRRQRANACGKSSGGQTQAGAPEEDDDDGLPSLDTEVWSWGRGSEGQLGHGDQLARLQPLCIKTLSGEEVIQVAAGSHHSLAVTATCQVYSWGSNMCGQLGHDNSSFVVPQQAKLSDGLRVWDMSAGQSHSLLLADGDCVQPVLLYCGRQKESTSDQREKSYQRSPRKAEHYTVRPTLLPVCLEMGYISSVCSGGQSCAVLADQNIKVFISALHELASRERQFYCWLSRVRKLVLTPIQNKESTSPFLGDPCTHLFSSLCGSFSQLSVLIGRHSTSLTYFLQKAQGRDVTSLPLLTHMEQFLDTYKEYCSSVGDFQVMGGFQTLHKLTLECLQPEVLTQLCMSDQTSDDTGLVSLLYWPLQQLHQYDRVMVKVAFCFDVLMPEFQSFRQGCGQYESLSLSLLRKKKEAESTFLFWKSHSGKTTESLRLPRRRVVCESSSRSLTLQKAGRFSNHWFILFNDALVHTQFSTHHVYPLTCLWVKPVTDDSSGLYAIKIICPEESFTMVASTPQEKNKWLRSINHALDQVLGGSGEGSSPGTMGMSRTASYTFTGEGRFKDAKYSGSWMSGRVHGRGSLRWPDGRTYCGNFKNGLEDGYGECVIPNKELNKPDSYHGHWREGKIHGFGKYKYASGEVYEGCFSEGQRQGYGMLSSGKLGKKSSSVFIGHWVHDKKTGYGVFDDITRGEKYMGLWVDEKRHGSGVVVTQYGVYYEGTFRDNKMSGPGLLMSDDDTAIHGEFSDDWVVNGKGVLLLANGDCLEGLFSGEWTSGLKVDGTYTKPLVDEPVNKERKNMFKLGRYVVPAGQRWVCVFEECWSRLGCDSTGRGDKNVAWENIAVSITAARRQSPDLSRSQNKVLESLEFIPQHEQPVTTSNYDSIRRYLIKACETPLHPLGWLVETLVTVYRMTYVGVEANRRLLEQAVQELQAYLTHLYNIVQFLFPGMPDDGAVIPDPPGSPSVVQNSSSMEDLSAVVVSCSSLLLPLLLPRLYPPLFTLYRLQEEQEDALYWDRILRLNKQPDQSLLSFLGVQEKFWPTWMSILGEKKQIVSSSKDACFVSAVETLQQISTTFTPSDKLVVIQKTFEELTQEVNPMLDGHFLWCMDDLLPLFIYVVVRARIRNLGAEVDMIEDLMDPNVQHGELGLMFTTLKACYIQIKNESTT

Target ORF information:

RefSeq Version	XM_011611399.1
Organism	Takifugu rubripes(Fugu rubripes)
Definition	Takifugu rubripes alsin-like (LOC101078293), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011611399.1

ORF Insert Sequence:

ATGGAGAACG CAGAGGACAG CCCAGCAGAT GAGTTGGTCT CCAGAGCTCC AGAGAGTGGA 
CTTTTCCACA TCTGGCAGTC AGCAGGCCCC AGTGCCCAGC TGTGTCCGGA GAGAGTGCTG 
CTGTCTATTC CTGTTGTGCA GGCTGCCCTG GGAGAGCATC ATGGAGTCCT CTTGGTGCAG 
GGGGGCCTCG TCTATTCATT TGGAGAGCTC TTGTGGAAAG ATCTGAGTGT CCCCGCATCT 
GCTCCAGTCT TGGAAGGCTC TCTGCTGGGA CGGGTGGTGG TCCGGGTGGC TGCAGGCGGT 
TTCCACTGTG GCGCTGTGAG CGAACAGGGA CATGTGTACA CATGGGGGGA GAACACTGGA 
GGACAGTGTG GGCTGACAGA GCGGACTACA GAAAAAACCA TCACAGTTTC AGAGCCTTCT 
CTTGTTAGCG TGGTCGACCA TGAGGTCATC CCGCCAGCGG CTGTTAGGGT TGTGGATCTT 
GCTTTTGGAC GCGAGCACAG TCTGGCTCTG TCAGCCCAGA ACGAGCTGTG GGCGTGGGGC 
AGCGGCTGCC AGCTTGGCCT GGTCACCACC ACCTTCCCTG TGTGTAGACC ACAGAAGGTG 
GAGCATTTAG CAGGCAGACA TGTTATTCAG GTTGTTTGCG GTGCCTACCA CAGCTTGGCC 
CTGGTTCGTA GTTTGCCTCC AAATAGCTAC AACAGCCAGA AGCCTTCTGA AAAAAGGGAA 
CGGGGTCACT CGCCGCACTA TTCAGCAGCA GAGAGAGAGG AGCTGTTTGT GGGTGACAGC 
AGTCTCTACT GTCCACTTGG GGTGGAGCTG AAAGAAGGCA TGGCCACAGA GACTTCCTCC 
AGGAGATGTC CAAGGAGAAG GCTCCATCCT GGAGGAAGTT CGGCCAGCAG CAGCCCTGGC 
TCTGCATTTA ACGAAGACGC GACCAAAGAC ACTGACTCAC TGAGCAGCTG CACCTCAGAT 
GACAGCTGTG TTTCCTCAAC TCTCTCCACT GATATGCTGA CCTCCAGTTA CAAAGAACCT 
AACAATAACT TTGGAGTGAC CCTGCCGTGC TCGTCCTCCT CACTGTGTCT CGACGATGTC 
CGACTCTGTC TTGAATCGGG GCTGCAGGTA CAGCCAGGGA AGAAGCTCTC CAGCCTCTCC 
AATAAACACT ATAAAGCAAA ATCAGCAGGA ACCAGAAGAC ATTCTGTCCC TGGGACACCC 
ACTCGTGGGT CTCCACGGCG ACAGCGTGCA AACGCGTGCG GAAAATCTTC TGGTGGTCAG 
ACCCAGGCGG GAGCGCCGGA GGAGGATGAT GATGATGGGC TGCCATCTCT AGACACAGAA 
GTCTGGAGCT GGGGACGGGG CTCTGAGGGG CAGCTCGGCC ACGGAGATCA GCTCGCCAGA 
CTTCAGCCTC TGTGTATCAA GACACTGTCG GGTGAGGAGG TGATCCAAGT TGCAGCAGGG 
TCCCATCATT CTCTGGCTGT CACTGCAACC TGTCAGGTGT ACTCATGGGG CAGCAACATG 
TGTGGGCAGC TGGGTCACGA TAACAGTTCT TTCGTTGTGC CCCAACAGGC AAAGCTGTCT 
GATGGCCTCC GAGTTTGGGA CATGTCTGCC GGCCAGAGCC ACTCCCTCCT CCTGGCTGAT 
GGAGACTGCG TCCAGCCCGT GTTGTTGTAC TGTGGCCGGC AGAAAGAGTC GACATCAGAC 
CAGAGAGAAA AGTCCTACCA GAGGTCCCCC AGAAAAGCAG AGCATTACAC AGTCAGGCCC 
ACCCTGCTGC CTGTCTGCCT GGAGATGGGC TACATCAGCA GTGTGTGCAG CGGCGGGCAA 
AGCTGCGCCG TCCTGGCAGA CCAGAACATC AAGGTTTTTA TTTCGGCCCT CCACGAGCTG 
GCCTCCAGAG AGAGGCAGTT CTACTGCTGG TTGAGTCGCG TCAGGAAACT CGTCCTCACA 
CCAATACAGA ACAAAGAGAG TACGTCTCCG TTTTTAGGTG ACCCTTGCAC CCATCTCTTT 
TCTTCTCTCT GTGGGAGTTT TTCTCAGCTA AGCGTCCTAA TCGGTCGACA CTCCACATCG 
CTTACCTACT TCCTGCAAAA GGCACAGGGC CGTGACGTCA CCTCACTCCC CCTGCTCACA 
CACATGGAGC AGTTTCTGGA CACTTACAAA GAATATTGTT CCTCTGTTGG TGACTTTCAG 
GTGATGGGGG GCTTCCAGAC CCTCCATAAG CTCACACTTG AGTGTTTGCA GCCAGAAGTT 
CTCACTCAGC TGTGTATGTC GGACCAGACC AGTGATGACA CTGGTCTGGT TTCGCTGTTG 
TACTGGCCTC TGCAGCAGCT CCACCAGTAC GACCGAGTCA TGGTCAAAGT GGCGTTTTGC 
TTCGACGTGT TAATGCCAGA GTTTCAGTCC TTCCGTCAGG GCTGCGGTCA GTACGAGTCC 
CTGTCTCTGT CCCTGTTGAG GAAGAAAAAA GAGGCTGAAA GCACATTCCT CTTCTGGAAG 
AGCCACTCCG GAAAAACTAC TGAGAGTCTA CGTCTCCCTC GGCGCCGAGT GGTGTGTGAA 
AGCAGCAGCA GATCTTTGAC TCTGCAAAAG GCCGGGCGCT TCTCCAACCA CTGGTTCATT 
CTGTTCAATG ATGCACTGGT CCACACGCAG TTCTCCACAC ATCACGTCTA CCCCCTGACC 
TGCCTGTGGG TGAAACCCGT CACTGACGAC AGCAGCGGAC TCTATGCCAT TAAAATTATT 
TGCCCGGAGG AAAGTTTCAC CATGGTGGCT TCAACACCTC AAGAAAAGAA CAAGTGGTTA 
CGATCTATCA ATCACGCGCT GGATCAGGTG CTCGGTGGTA GTGGTGAGGG GTCGTCGCCG 
GGGACGATGG GAATGTCCCG CACGGCCTCC TACACATTCA CCGGAGAAGG CCGGTTTAAA 
GATGCCAAAT ACAGCGGCAG CTGGATGTCA GGACGGGTGC ATGGCAGAGG GTCCTTGAGG 
TGGCCTGATG GGCGGACCTA CTGCGGGAAC TTCAAGAACG GCCTTGAAGA TGGTTACGGG 
GAGTGCGTAA TACCCAACAA AGAGCTGAAC AAGCCTGACA GTTACCATGG ACACTGGAGA 
GAGGGGAAGA TCCACGGTTT CGGCAAATAC AAGTACGCCA GCGGCGAGGT GTATGAAGGC 
TGTTTCAGTG AAGGGCAGCG GCAGGGTTAC GGCATGCTGA GCTCCGGGAA GCTGGGGAAG 
AAGTCCTCCA GTGTTTTCAT CGGCCACTGG GTCCATGACA AGAAGACAGG TTATGGAGTG 
TTTGACGACA TCACCAGGGG TGAGAAGTAC ATGGGATTGT GGGTAGACGA GAAGCGGCAC 
GGCAGCGGCG TGGTGGTCAC ACAGTATGGC GTGTACTACG AAGGAACCTT CAGAGACAAC 
AAGATGAGTG GTCCCGGTCT GCTGATGTCG GATGACGACA CGGCTATTCA TGGGGAGTTT 
TCCGACGATT GGGTTGTCAA CGGGAAGGGT GTTTTATTGT TGGCCAACGG AGACTGTCTG 
GAGGGTCTGT TCAGTGGGGA GTGGACATCT GGACTGAAGG TGGATGGAAC GTACACCAAA 
CCACTGGTGG ACGAGCCTGT AAACAAAGAA AGAAAAAACA TGTTCAAGCT GGGTAGGTAC 
GTGGTGCCTG CGGGTCAGAG GTGGGTCTGT GTGTTTGAGG AGTGCTGGAG TCGACTGGGC 
TGCGACAGCA CTGGGAGAGG AGACAAAAAT GTAGCCTGGG AGAACATCGC AGTGTCCATA 
ACGGCTGCAC GGAGGCAAAG TCCAGATCTC AGTCGGTCTC AGAACAAAGT GTTGGAGTCT 
CTGGAGTTTA TCCCTCAGCA TGAGCAGCCA GTCACCACCT CGAACTACGA CAGCATACGC 
AGATACCTCA TCAAGGCTTG TGAGACCCCC CTCCACCCTC TGGGCTGGCT GGTGGAGACT 
CTGGTGACGG TGTACCGGAT GACGTATGTG GGCGTGGAAG CTAACCGGAG GCTGCTGGAA 
CAGGCTGTCC AGGAGCTTCA GGCCTACCTC ACACATCTCT ACAACATTGT CCAGTTTTTG 
TTTCCAGGGA TGCCAGATGA TGGCGCCGTC ATCCCAGACC CCCCGGGGTC TCCATCGGTA 
GTCCAGAACA GCTCCAGCAT GGAAGACTTG AGTGCTGTAG TGGTGAGCTG CTCCTCTCTG 
CTCCTCCCTC TGCTCCTGCC TCGACTCTAC CCTCCTCTCT TCACGCTGTA CCGGCTGCAG 
GAGGAGCAGG AAGACGCACT GTACTGGGAC CGCATCCTGC GTCTGAACAA ACAACCCGAT 
CAGTCGCTGC TGAGCTTCCT GGGAGTGCAA GAGAAGTTCT GGCCTACGTG GATGTCAATT 
TTGGGGGAGA AGAAGCAGAT TGTGTCGAGC AGCAAAGACG CCTGCTTCGT TTCCGCAGTT 
GAGACCCTCC AACAAATCAG CACCACCTTT ACTCCATCTG ACAAACTCGT CGTCATCCAG 
AAGACCTTTG AGGAGTTGAC CCAGGAAGTC AATCCCATGC TGGACGGCCA CTTCCTGTGG 
TGCATGGATG ACTTGCTCCC ACTCTTCATC TACGTGGTGG TCAGAGCGAG GATCAGGAAT 
CTGGGAGCGG AGGTCGATAT GATTGAAGAT TTGATGGATC CGAATGTTCA GCATGGAGAG 
CTGGGACTGA TGTTCACCAC CCTGAAGGCC TGCTACATCC AGATCAAAAA CGAGTCTACG 
ACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101078293 ( XM_011611404.1 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_011609706.1 Takifugu rubripes alsin-like (LOC101078293), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OTo30995
Clone ID Related Accession (Same CDS sequence)	XM_011611404.1 , XM_011611411.1
Accession Version	XM_011611404.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4068bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-15
Organism	Takifugu rubripes(Fugu rubripes)
Product	alsin-like isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004070909.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Takifugu rubripes Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTATTCA GCTTGGCCCT GGTTCGTAGT TTGCCTCCAA ATAGCTACAA CAGCCAGAAG 
CCTTCTGAAA AAAGGGAACG GGGTCACTCG CCGCACTATT CAGCAGCAGA GAGAGAGGAG 
CTGTTTGTGG GTGACAGCAG TCTCTACTGT CCACTTGGGG TGGAGCTGAA AGAAGGCATG 
GCCACAGAGA CTTCCTCCAG GAGATGTCCA AGGAGAAGGC TCCATCCTGG AGGAAGTTCG 
GCCAGCAGCA GCCCTGGCTC TGCATTTAAC GAAGACGCGA CCAAAGACAC TGACTCACTG 
AGCAGCTGCA CCTCAGATGA CAGCTGTGTT TCCTCAACTC TCTCCACTGA TATGCTGACC 
TCCAGTTACA AAGAACCTAA CAATAACTTT GGAGTGACCC TGCCGTGCTC GTCCTCCTCA 
CTGTGTCTCG ACGATGTCCG ACTCTGTCTT GAATCGGGGC TGCAGGTACA GCCAGGGAAG 
AAGCTCTCCA GCCTCTCCAA TAAACACTAT AAAGCAAAAT CAGCAGGAAC CAGAAGACAT 
TCTGTCCCTG GGACACCCAC TCGTGGGTCT CCACGGCGAC AGCGTGCAAA CGCGTGCGGA 
AAATCTTCTG GTGGTCAGAC CCAGGCGGGA GCGCCGGAGG AGGATGATGA TGATGGGCTG 
CCATCTCTAG ACACAGAAGT CTGGAGCTGG GGACGGGGCT CTGAGGGGCA GCTCGGCCAC 
GGAGATCAGC TCGCCAGACT TCAGCCTCTG TGTATCAAGA CACTGTCGGG TGAGGAGGTG 
ATCCAAGTTG CAGCAGGGTC CCATCATTCT CTGGCTGTCA CTGCAACCTG TCAGGTGTAC 
TCATGGGGCA GCAACATGTG TGGGCAGCTG GGTCACGATA ACAGTTCTTT CGTTGTGCCC 
CAACAGGCAA AGCTGTCTGA TGGCCTCCGA GTTTGGGACA TGTCTGCCGG CCAGAGCCAC 
TCCCTCCTCC TGGCTGATGG AGACTGCGTC CAGCCCGTGT TGTTGTACTG TGGCCGGCAG 
AAAGAGTCGA CATCAGACCA GAGAGAAAAG TCCTACCAGA GGTCCCCCAG AAAAGCAGAG 
CATTACACAG TCAGGCCCAC CCTGCTGCCT GTCTGCCTGG AGATGGGCTA CATCAGCAGT 
GTGTGCAGCG GCGGGCAAAG CTGCGCCGTC CTGGCAGACC AGAACATCAA GGTTTTTATT 
TCGGCCCTCC ACGAGCTGGC CTCCAGAGAG AGGCAGTTCT ACTGCTGGTT GAGTCGCGTC 
AGGAAACTCG TCCTCACACC AATACAGAAC AAAGAGAGTA CGTCTCCGTT TTTAGGTGAC 
CCTTGCACCC ATCTCTTTTC TTCTCTCTGT GGGAGTTTTT CTCAGCTAAG CGTCCTAATC 
GGTCGACACT CCACATCGCT TACCTACTTC CTGCAAAAGG CACAGGGCCG TGACGTCACC 
TCACTCCCCC TGCTCACACA CATGGAGCAG TTTCTGGACA CTTACAAAGA ATATTGTTCC 
TCTGTTGGTG ACTTTCAGGT GATGGGGGGC TTCCAGACCC TCCATAAGCT CACACTTGAG 
TGTTTGCAGC CAGAAGTTCT CACTCAGCTG TGTATGTCGG ACCAGACCAG TGATGACACT 
GGTCTGGTTT CGCTGTTGTA CTGGCCTCTG CAGCAGCTCC ACCAGTACGA CCGAGTCATG 
GTCAAAGTGG CGTTTTGCTT CGACGTGTTA ATGCCAGAGT TTCAGTCCTT CCGTCAGGGC 
TGCGGTCAGT ACGAGTCCCT GTCTCTGTCC CTGTTGAGGA AGAAAAAAGA GGCTGAAAGC 
ACATTCCTCT TCTGGAAGAG CCACTCCGGA AAAACTACTG AGAGTCTACG TCTCCCTCGG 
CGCCGAGTGG TGTGTGAAAG CAGCAGCAGA TCTTTGACTC TGCAAAAGGC CGGGCGCTTC 
TCCAACCACT GGTTCATTCT GTTCAATGAT GCACTGGTCC ACACGCAGGG CGCGCTTCCC 
TCACAGCTCT TTTTCTCCAC ACATCACGTC TACCCCCTGA CCTGCCTGTG GGTGAAACCC 
GTCACTGACG ACAGCAGCGG ACTCTATGCC ATTAAAATTA TTTGCCCGGA GGAAAGTTTC 
ACCATGGTGG CTTCAACACC TCAAGAAAAG AACAAGTGGT TACGATCTAT CAATCACGCG 
CTGGATCAGG TGCTCGGTGG TAGTGGTGAG GGGTCGTCGC CGGGGACGAT GGGAATGTCC 
CGCACGGCCT CCTACACATT CACCGGAGAA GGCCGGTTTA AAGATGCCAA ATACAGCGGC 
AGCTGGATGT CAGGACGGGT GCATGGCAGA GGGTCCTTGA GGTGGCCTGA TGGGCGGACC 
TACTGCGGGA ACTTCAAGAA CGGCCTTGAA GATGGTTACG GGGAGTGCGT AATACCCAAC 
AAAGAGCTGA ACAAGCCTGA CAGTTACCAT GGACACTGGA GAGAGGGGAA GATCCACGGT 
TTCGGCAAAT ACAAGTACGC CAGCGGCGAG GTGTATGAAG GCTGTTTCAG TGAAGGGCAG 
CGGCAGGGTT ACGGCATGCT GAGCTCCGGG AAGCTGGGGA AGAAGTCCTC CAGTGTTTTC 
ATCGGCCACT GGGTCCATGA CAAGAAGACA GGTTATGGAG TGTTTGACGA CATCACCAGG 
GGTGAGAAGT ACATGGGATT GTGGGTAGAC GAGAAGCGGC ACGGCAGCGG CGTGGTGGTC 
ACACAGTATG GCGTGTACTA CGAAGGAACC TTCAGAGACA ACAAGATGAG TGGTCCCGGT 
CTGCTGATGT CGGATGACGA CACGGCTATT CATGGGGAGT TTTCCGACGA TTGGGTTGTC 
AACGGGAAGG GTGTTTTATT GTTGGCCAAC GGAGACTGTC TGGAGGGTCT GTTCAGTGGG 
GAGTGGACAT CTGGACTGAA GGTGGATGGA ACGTACACCA AACCACTGGT GGACGAGCCT 
GTAAACAAAG AAAGAAAAAA CATGTTCAAG CTGGGTAGGT ACGTGGTGCC TGCGGGTCAG 
AGGTGGGTCT GTGTGTTTGA GGAGTGCTGG AGTCGACTGG GCTGCGACAG CACTGGGAGA 
GGAGACAAAA ATGTAGCCTG GGAGAACATC GCAGTGTCCA TAACGGCTGC ACGGAGGCAA 
AGTCCAGATC TCAGTCGGTC TCAGAACAAA GTGTTGGAGT CTCTGGAGTT TATCCCTCAG 
CATGAGCAGC CAGTCACCAC CTCGAACTAC GACAGCATAC GCAGATACCT CATCAAGGCT 
TGTGAGACCC CCCTCCACCC TCTGGGCTGG CTGGTGGAGA CTCTGGTGAC GGTGTACCGG 
ATGACGTATG TGGGCGTGGA AGCTAACCGG AGGCTGCTGG AACAGGCTGT CCAGGAGCTT 
CAGGCCTACC TCACACATCT CTACAACATT GTCCAGTTTT TGTTTCCAGG GATGCCAGAT 
GATGGCGCCG TCATCCCAGA CCCCCCGGGG TCTCCATCGG TAGTCCAGAA CAGCTCCAGC 
ATGGAAGACT TGAGTGCTGT AGTGGTGAGC TGCTCCTCTC TGCTCCTCCC TCTGCTCCTG 
CCTCGACTCT ACCCTCCTCT CTTCACGCTG TACCGGCTGC AGGAGGAGCA GGAAGACGCA 
CTGTACTGGG ACCGCATCCT GCGTCTGAAC AAACAACCCG ATCAGTCGCT GCTGAGCTTC 
CTGGGAGTGC AAGAGAAGTT CTGGCCTACG TGGATGTCAA TTTTGGGGGA GAAGAAGCAG 
ATTGTGTCGA GCAGCAAAGA CGCCTGCTTC GTTTCCGCAG TTGAGACCCT CCAACAAATC 
AGCACCACCT TTACTCCATC TGACAAACTC GTCGTCATCC AGAAGACCTT TGAGGAGTTG 
ACCCAGGAAG TCAATCCCAT GCTGGACGGC CACTTCCTGT GGTGCATGGA TGACTTGCTC 
CCACTCTTCA TCTACGTGGT GGTCAGAGCG AGGATCAGGA ATCTGGGAGC GGAGGTCGAT 
ATGATTGAAG ATTTGATGGA TCCGAATGTT CAGCATGGAG AGCTGGGACT GATGTTCACC 
ACCCTGAAGG CCTGCTACAT CCAGATCAAA AACGAGTCTA CGACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011609706.1
CDS	120..4187
Translation	MLFSLALVRSLPPNSYNSQKPSEKRERGHSPHYSAAEREELFVGDSSLYCPLGVELKEGMATETSSRRCPRRRLHPGGSSASSSPGSAFNEDATKDTDSLSSCTSDDSCVSSTLSTDMLTSSYKEPNNNFGVTLPCSSSSLCLDDVRLCLESGLQVQPGKKLSSLSNKHYKAKSAGTRRHSVPGTPTRGSPRRQRANACGKSSGGQTQAGAPEEDDDDGLPSLDTEVWSWGRGSEGQLGHGDQLARLQPLCIKTLSGEEVIQVAAGSHHSLAVTATCQVYSWGSNMCGQLGHDNSSFVVPQQAKLSDGLRVWDMSAGQSHSLLLADGDCVQPVLLYCGRQKESTSDQREKSYQRSPRKAEHYTVRPTLLPVCLEMGYISSVCSGGQSCAVLADQNIKVFISALHELASRERQFYCWLSRVRKLVLTPIQNKESTSPFLGDPCTHLFSSLCGSFSQLSVLIGRHSTSLTYFLQKAQGRDVTSLPLLTHMEQFLDTYKEYCSSVGDFQVMGGFQTLHKLTLECLQPEVLTQLCMSDQTSDDTGLVSLLYWPLQQLHQYDRVMVKVAFCFDVLMPEFQSFRQGCGQYESLSLSLLRKKKEAESTFLFWKSHSGKTTESLRLPRRRVVCESSSRSLTLQKAGRFSNHWFILFNDALVHTQGALPSQLFFSTHHVYPLTCLWVKPVTDDSSGLYAIKIICPEESFTMVASTPQEKNKWLRSINHALDQVLGGSGEGSSPGTMGMSRTASYTFTGEGRFKDAKYSGSWMSGRVHGRGSLRWPDGRTYCGNFKNGLEDGYGECVIPNKELNKPDSYHGHWREGKIHGFGKYKYASGEVYEGCFSEGQRQGYGMLSSGKLGKKSSSVFIGHWVHDKKTGYGVFDDITRGEKYMGLWVDEKRHGSGVVVTQYGVYYEGTFRDNKMSGPGLLMSDDDTAIHGEFSDDWVVNGKGVLLLANGDCLEGLFSGEWTSGLKVDGTYTKPLVDEPVNKERKNMFKLGRYVVPAGQRWVCVFEECWSRLGCDSTGRGDKNVAWENIAVSITAARRQSPDLSRSQNKVLESLEFIPQHEQPVTTSNYDSIRRYLIKACETPLHPLGWLVETLVTVYRMTYVGVEANRRLLEQAVQELQAYLTHLYNIVQFLFPGMPDDGAVIPDPPGSPSVVQNSSSMEDLSAVVVSCSSLLLPLLLPRLYPPLFTLYRLQEEQEDALYWDRILRLNKQPDQSLLSFLGVQEKFWPTWMSILGEKKQIVSSSKDACFVSAVETLQQISTTFTPSDKLVVIQKTFEELTQEVNPMLDGHFLWCMDDLLPLFIYVVVRARIRNLGAEVDMIEDLMDPNVQHGELGLMFTTLKACYIQIKNESTT

Target ORF information:

RefSeq Version	XM_011611404.1
Organism	Takifugu rubripes(Fugu rubripes)
Definition	Takifugu rubripes alsin-like (LOC101078293), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011611404.1

ORF Insert Sequence:

ATGTTATTCA GCTTGGCCCT GGTTCGTAGT TTGCCTCCAA ATAGCTACAA CAGCCAGAAG 
CCTTCTGAAA AAAGGGAACG GGGTCACTCG CCGCACTATT CAGCAGCAGA GAGAGAGGAG 
CTGTTTGTGG GTGACAGCAG TCTCTACTGT CCACTTGGGG TGGAGCTGAA AGAAGGCATG 
GCCACAGAGA CTTCCTCCAG GAGATGTCCA AGGAGAAGGC TCCATCCTGG AGGAAGTTCG 
GCCAGCAGCA GCCCTGGCTC TGCATTTAAC GAAGACGCGA CCAAAGACAC TGACTCACTG 
AGCAGCTGCA CCTCAGATGA CAGCTGTGTT TCCTCAACTC TCTCCACTGA TATGCTGACC 
TCCAGTTACA AAGAACCTAA CAATAACTTT GGAGTGACCC TGCCGTGCTC GTCCTCCTCA 
CTGTGTCTCG ACGATGTCCG ACTCTGTCTT GAATCGGGGC TGCAGGTACA GCCAGGGAAG 
AAGCTCTCCA GCCTCTCCAA TAAACACTAT AAAGCAAAAT CAGCAGGAAC CAGAAGACAT 
TCTGTCCCTG GGACACCCAC TCGTGGGTCT CCACGGCGAC AGCGTGCAAA CGCGTGCGGA 
AAATCTTCTG GTGGTCAGAC CCAGGCGGGA GCGCCGGAGG AGGATGATGA TGATGGGCTG 
CCATCTCTAG ACACAGAAGT CTGGAGCTGG GGACGGGGCT CTGAGGGGCA GCTCGGCCAC 
GGAGATCAGC TCGCCAGACT TCAGCCTCTG TGTATCAAGA CACTGTCGGG TGAGGAGGTG 
ATCCAAGTTG CAGCAGGGTC CCATCATTCT CTGGCTGTCA CTGCAACCTG TCAGGTGTAC 
TCATGGGGCA GCAACATGTG TGGGCAGCTG GGTCACGATA ACAGTTCTTT CGTTGTGCCC 
CAACAGGCAA AGCTGTCTGA TGGCCTCCGA GTTTGGGACA TGTCTGCCGG CCAGAGCCAC 
TCCCTCCTCC TGGCTGATGG AGACTGCGTC CAGCCCGTGT TGTTGTACTG TGGCCGGCAG 
AAAGAGTCGA CATCAGACCA GAGAGAAAAG TCCTACCAGA GGTCCCCCAG AAAAGCAGAG 
CATTACACAG TCAGGCCCAC CCTGCTGCCT GTCTGCCTGG AGATGGGCTA CATCAGCAGT 
GTGTGCAGCG GCGGGCAAAG CTGCGCCGTC CTGGCAGACC AGAACATCAA GGTTTTTATT 
TCGGCCCTCC ACGAGCTGGC CTCCAGAGAG AGGCAGTTCT ACTGCTGGTT GAGTCGCGTC 
AGGAAACTCG TCCTCACACC AATACAGAAC AAAGAGAGTA CGTCTCCGTT TTTAGGTGAC 
CCTTGCACCC ATCTCTTTTC TTCTCTCTGT GGGAGTTTTT CTCAGCTAAG CGTCCTAATC 
GGTCGACACT CCACATCGCT TACCTACTTC CTGCAAAAGG CACAGGGCCG TGACGTCACC 
TCACTCCCCC TGCTCACACA CATGGAGCAG TTTCTGGACA CTTACAAAGA ATATTGTTCC 
TCTGTTGGTG ACTTTCAGGT GATGGGGGGC TTCCAGACCC TCCATAAGCT CACACTTGAG 
TGTTTGCAGC CAGAAGTTCT CACTCAGCTG TGTATGTCGG ACCAGACCAG TGATGACACT 
GGTCTGGTTT CGCTGTTGTA CTGGCCTCTG CAGCAGCTCC ACCAGTACGA CCGAGTCATG 
GTCAAAGTGG CGTTTTGCTT CGACGTGTTA ATGCCAGAGT TTCAGTCCTT CCGTCAGGGC 
TGCGGTCAGT ACGAGTCCCT GTCTCTGTCC CTGTTGAGGA AGAAAAAAGA GGCTGAAAGC 
ACATTCCTCT TCTGGAAGAG CCACTCCGGA AAAACTACTG AGAGTCTACG TCTCCCTCGG 
CGCCGAGTGG TGTGTGAAAG CAGCAGCAGA TCTTTGACTC TGCAAAAGGC CGGGCGCTTC 
TCCAACCACT GGTTCATTCT GTTCAATGAT GCACTGGTCC ACACGCAGGG CGCGCTTCCC 
TCACAGCTCT TTTTCTCCAC ACATCACGTC TACCCCCTGA CCTGCCTGTG GGTGAAACCC 
GTCACTGACG ACAGCAGCGG ACTCTATGCC ATTAAAATTA TTTGCCCGGA GGAAAGTTTC 
ACCATGGTGG CTTCAACACC TCAAGAAAAG AACAAGTGGT TACGATCTAT CAATCACGCG 
CTGGATCAGG TGCTCGGTGG TAGTGGTGAG GGGTCGTCGC CGGGGACGAT GGGAATGTCC 
CGCACGGCCT CCTACACATT CACCGGAGAA GGCCGGTTTA AAGATGCCAA ATACAGCGGC 
AGCTGGATGT CAGGACGGGT GCATGGCAGA GGGTCCTTGA GGTGGCCTGA TGGGCGGACC 
TACTGCGGGA ACTTCAAGAA CGGCCTTGAA GATGGTTACG GGGAGTGCGT AATACCCAAC 
AAAGAGCTGA ACAAGCCTGA CAGTTACCAT GGACACTGGA GAGAGGGGAA GATCCACGGT 
TTCGGCAAAT ACAAGTACGC CAGCGGCGAG GTGTATGAAG GCTGTTTCAG TGAAGGGCAG 
CGGCAGGGTT ACGGCATGCT GAGCTCCGGG AAGCTGGGGA AGAAGTCCTC CAGTGTTTTC 
ATCGGCCACT GGGTCCATGA CAAGAAGACA GGTTATGGAG TGTTTGACGA CATCACCAGG 
GGTGAGAAGT ACATGGGATT GTGGGTAGAC GAGAAGCGGC ACGGCAGCGG CGTGGTGGTC 
ACACAGTATG GCGTGTACTA CGAAGGAACC TTCAGAGACA ACAAGATGAG TGGTCCCGGT 
CTGCTGATGT CGGATGACGA CACGGCTATT CATGGGGAGT TTTCCGACGA TTGGGTTGTC 
AACGGGAAGG GTGTTTTATT GTTGGCCAAC GGAGACTGTC TGGAGGGTCT GTTCAGTGGG 
GAGTGGACAT CTGGACTGAA GGTGGATGGA ACGTACACCA AACCACTGGT GGACGAGCCT 
GTAAACAAAG AAAGAAAAAA CATGTTCAAG CTGGGTAGGT ACGTGGTGCC TGCGGGTCAG 
AGGTGGGTCT GTGTGTTTGA GGAGTGCTGG AGTCGACTGG GCTGCGACAG CACTGGGAGA 
GGAGACAAAA ATGTAGCCTG GGAGAACATC GCAGTGTCCA TAACGGCTGC ACGGAGGCAA 
AGTCCAGATC TCAGTCGGTC TCAGAACAAA GTGTTGGAGT CTCTGGAGTT TATCCCTCAG 
CATGAGCAGC CAGTCACCAC CTCGAACTAC GACAGCATAC GCAGATACCT CATCAAGGCT 
TGTGAGACCC CCCTCCACCC TCTGGGCTGG CTGGTGGAGA CTCTGGTGAC GGTGTACCGG 
ATGACGTATG TGGGCGTGGA AGCTAACCGG AGGCTGCTGG AACAGGCTGT CCAGGAGCTT 
CAGGCCTACC TCACACATCT CTACAACATT GTCCAGTTTT TGTTTCCAGG GATGCCAGAT 
GATGGCGCCG TCATCCCAGA CCCCCCGGGG TCTCCATCGG TAGTCCAGAA CAGCTCCAGC 
ATGGAAGACT TGAGTGCTGT AGTGGTGAGC TGCTCCTCTC TGCTCCTCCC TCTGCTCCTG 
CCTCGACTCT ACCCTCCTCT CTTCACGCTG TACCGGCTGC AGGAGGAGCA GGAAGACGCA 
CTGTACTGGG ACCGCATCCT GCGTCTGAAC AAACAACCCG ATCAGTCGCT GCTGAGCTTC 
CTGGGAGTGC AAGAGAAGTT CTGGCCTACG TGGATGTCAA TTTTGGGGGA GAAGAAGCAG 
ATTGTGTCGA GCAGCAAAGA CGCCTGCTTC GTTTCCGCAG TTGAGACCCT CCAACAAATC 
AGCACCACCT TTACTCCATC TGACAAACTC GTCGTCATCC AGAAGACCTT TGAGGAGTTG 
ACCCAGGAAG TCAATCCCAT GCTGGACGGC CACTTCCTGT GGTGCATGGA TGACTTGCTC 
CCACTCTTCA TCTACGTGGT GGTCAGAGCG AGGATCAGGA ATCTGGGAGC GGAGGTCGAT 
ATGATTGAAG ATTTGATGGA TCCGAATGTT CAGCATGGAG AGCTGGGACT GATGTTCACC 
ACCCTGAAGG CCTGCTACAT CCAGATCAAA AACGAGTCTA CGACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101078293 ( XM_011611411.1 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_011609713.1 Takifugu rubripes alsin-like (LOC101078293), transcript variant X4, mRNA.

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CloneID	OTo30995
Clone ID Related Accession (Same CDS sequence)	XM_011611404.1 , XM_011611411.1
Accession Version	XM_011611411.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4068bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-15
Organism	Takifugu rubripes(Fugu rubripes)
Product	alsin-like isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004070909.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Takifugu rubripes Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTATTCA GCTTGGCCCT GGTTCGTAGT TTGCCTCCAA ATAGCTACAA CAGCCAGAAG 
CCTTCTGAAA AAAGGGAACG GGGTCACTCG CCGCACTATT CAGCAGCAGA GAGAGAGGAG 
CTGTTTGTGG GTGACAGCAG TCTCTACTGT CCACTTGGGG TGGAGCTGAA AGAAGGCATG 
GCCACAGAGA CTTCCTCCAG GAGATGTCCA AGGAGAAGGC TCCATCCTGG AGGAAGTTCG 
GCCAGCAGCA GCCCTGGCTC TGCATTTAAC GAAGACGCGA CCAAAGACAC TGACTCACTG 
AGCAGCTGCA CCTCAGATGA CAGCTGTGTT TCCTCAACTC TCTCCACTGA TATGCTGACC 
TCCAGTTACA AAGAACCTAA CAATAACTTT GGAGTGACCC TGCCGTGCTC GTCCTCCTCA 
CTGTGTCTCG ACGATGTCCG ACTCTGTCTT GAATCGGGGC TGCAGGTACA GCCAGGGAAG 
AAGCTCTCCA GCCTCTCCAA TAAACACTAT AAAGCAAAAT CAGCAGGAAC CAGAAGACAT 
TCTGTCCCTG GGACACCCAC TCGTGGGTCT CCACGGCGAC AGCGTGCAAA CGCGTGCGGA 
AAATCTTCTG GTGGTCAGAC CCAGGCGGGA GCGCCGGAGG AGGATGATGA TGATGGGCTG 
CCATCTCTAG ACACAGAAGT CTGGAGCTGG GGACGGGGCT CTGAGGGGCA GCTCGGCCAC 
GGAGATCAGC TCGCCAGACT TCAGCCTCTG TGTATCAAGA CACTGTCGGG TGAGGAGGTG 
ATCCAAGTTG CAGCAGGGTC CCATCATTCT CTGGCTGTCA CTGCAACCTG TCAGGTGTAC 
TCATGGGGCA GCAACATGTG TGGGCAGCTG GGTCACGATA ACAGTTCTTT CGTTGTGCCC 
CAACAGGCAA AGCTGTCTGA TGGCCTCCGA GTTTGGGACA TGTCTGCCGG CCAGAGCCAC 
TCCCTCCTCC TGGCTGATGG AGACTGCGTC CAGCCCGTGT TGTTGTACTG TGGCCGGCAG 
AAAGAGTCGA CATCAGACCA GAGAGAAAAG TCCTACCAGA GGTCCCCCAG AAAAGCAGAG 
CATTACACAG TCAGGCCCAC CCTGCTGCCT GTCTGCCTGG AGATGGGCTA CATCAGCAGT 
GTGTGCAGCG GCGGGCAAAG CTGCGCCGTC CTGGCAGACC AGAACATCAA GGTTTTTATT 
TCGGCCCTCC ACGAGCTGGC CTCCAGAGAG AGGCAGTTCT ACTGCTGGTT GAGTCGCGTC 
AGGAAACTCG TCCTCACACC AATACAGAAC AAAGAGAGTA CGTCTCCGTT TTTAGGTGAC 
CCTTGCACCC ATCTCTTTTC TTCTCTCTGT GGGAGTTTTT CTCAGCTAAG CGTCCTAATC 
GGTCGACACT CCACATCGCT TACCTACTTC CTGCAAAAGG CACAGGGCCG TGACGTCACC 
TCACTCCCCC TGCTCACACA CATGGAGCAG TTTCTGGACA CTTACAAAGA ATATTGTTCC 
TCTGTTGGTG ACTTTCAGGT GATGGGGGGC TTCCAGACCC TCCATAAGCT CACACTTGAG 
TGTTTGCAGC CAGAAGTTCT CACTCAGCTG TGTATGTCGG ACCAGACCAG TGATGACACT 
GGTCTGGTTT CGCTGTTGTA CTGGCCTCTG CAGCAGCTCC ACCAGTACGA CCGAGTCATG 
GTCAAAGTGG CGTTTTGCTT CGACGTGTTA ATGCCAGAGT TTCAGTCCTT CCGTCAGGGC 
TGCGGTCAGT ACGAGTCCCT GTCTCTGTCC CTGTTGAGGA AGAAAAAAGA GGCTGAAAGC 
ACATTCCTCT TCTGGAAGAG CCACTCCGGA AAAACTACTG AGAGTCTACG TCTCCCTCGG 
CGCCGAGTGG TGTGTGAAAG CAGCAGCAGA TCTTTGACTC TGCAAAAGGC CGGGCGCTTC 
TCCAACCACT GGTTCATTCT GTTCAATGAT GCACTGGTCC ACACGCAGGG CGCGCTTCCC 
TCACAGCTCT TTTTCTCCAC ACATCACGTC TACCCCCTGA CCTGCCTGTG GGTGAAACCC 
GTCACTGACG ACAGCAGCGG ACTCTATGCC ATTAAAATTA TTTGCCCGGA GGAAAGTTTC 
ACCATGGTGG CTTCAACACC TCAAGAAAAG AACAAGTGGT TACGATCTAT CAATCACGCG 
CTGGATCAGG TGCTCGGTGG TAGTGGTGAG GGGTCGTCGC CGGGGACGAT GGGAATGTCC 
CGCACGGCCT CCTACACATT CACCGGAGAA GGCCGGTTTA AAGATGCCAA ATACAGCGGC 
AGCTGGATGT CAGGACGGGT GCATGGCAGA GGGTCCTTGA GGTGGCCTGA TGGGCGGACC 
TACTGCGGGA ACTTCAAGAA CGGCCTTGAA GATGGTTACG GGGAGTGCGT AATACCCAAC 
AAAGAGCTGA ACAAGCCTGA CAGTTACCAT GGACACTGGA GAGAGGGGAA GATCCACGGT 
TTCGGCAAAT ACAAGTACGC CAGCGGCGAG GTGTATGAAG GCTGTTTCAG TGAAGGGCAG 
CGGCAGGGTT ACGGCATGCT GAGCTCCGGG AAGCTGGGGA AGAAGTCCTC CAGTGTTTTC 
ATCGGCCACT GGGTCCATGA CAAGAAGACA GGTTATGGAG TGTTTGACGA CATCACCAGG 
GGTGAGAAGT ACATGGGATT GTGGGTAGAC GAGAAGCGGC ACGGCAGCGG CGTGGTGGTC 
ACACAGTATG GCGTGTACTA CGAAGGAACC TTCAGAGACA ACAAGATGAG TGGTCCCGGT 
CTGCTGATGT CGGATGACGA CACGGCTATT CATGGGGAGT TTTCCGACGA TTGGGTTGTC 
AACGGGAAGG GTGTTTTATT GTTGGCCAAC GGAGACTGTC TGGAGGGTCT GTTCAGTGGG 
GAGTGGACAT CTGGACTGAA GGTGGATGGA ACGTACACCA AACCACTGGT GGACGAGCCT 
GTAAACAAAG AAAGAAAAAA CATGTTCAAG CTGGGTAGGT ACGTGGTGCC TGCGGGTCAG 
AGGTGGGTCT GTGTGTTTGA GGAGTGCTGG AGTCGACTGG GCTGCGACAG CACTGGGAGA 
GGAGACAAAA ATGTAGCCTG GGAGAACATC GCAGTGTCCA TAACGGCTGC ACGGAGGCAA 
AGTCCAGATC TCAGTCGGTC TCAGAACAAA GTGTTGGAGT CTCTGGAGTT TATCCCTCAG 
CATGAGCAGC CAGTCACCAC CTCGAACTAC GACAGCATAC GCAGATACCT CATCAAGGCT 
TGTGAGACCC CCCTCCACCC TCTGGGCTGG CTGGTGGAGA CTCTGGTGAC GGTGTACCGG 
ATGACGTATG TGGGCGTGGA AGCTAACCGG AGGCTGCTGG AACAGGCTGT CCAGGAGCTT 
CAGGCCTACC TCACACATCT CTACAACATT GTCCAGTTTT TGTTTCCAGG GATGCCAGAT 
GATGGCGCCG TCATCCCAGA CCCCCCGGGG TCTCCATCGG TAGTCCAGAA CAGCTCCAGC 
ATGGAAGACT TGAGTGCTGT AGTGGTGAGC TGCTCCTCTC TGCTCCTCCC TCTGCTCCTG 
CCTCGACTCT ACCCTCCTCT CTTCACGCTG TACCGGCTGC AGGAGGAGCA GGAAGACGCA 
CTGTACTGGG ACCGCATCCT GCGTCTGAAC AAACAACCCG ATCAGTCGCT GCTGAGCTTC 
CTGGGAGTGC AAGAGAAGTT CTGGCCTACG TGGATGTCAA TTTTGGGGGA GAAGAAGCAG 
ATTGTGTCGA GCAGCAAAGA CGCCTGCTTC GTTTCCGCAG TTGAGACCCT CCAACAAATC 
AGCACCACCT TTACTCCATC TGACAAACTC GTCGTCATCC AGAAGACCTT TGAGGAGTTG 
ACCCAGGAAG TCAATCCCAT GCTGGACGGC CACTTCCTGT GGTGCATGGA TGACTTGCTC 
CCACTCTTCA TCTACGTGGT GGTCAGAGCG AGGATCAGGA ATCTGGGAGC GGAGGTCGAT 
ATGATTGAAG ATTTGATGGA TCCGAATGTT CAGCATGGAG AGCTGGGACT GATGTTCACC 
ACCCTGAAGG CCTGCTACAT CCAGATCAAA AACGAGTCTA CGACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011609713.1
CDS	69..4136
Translation	MLFSLALVRSLPPNSYNSQKPSEKRERGHSPHYSAAEREELFVGDSSLYCPLGVELKEGMATETSSRRCPRRRLHPGGSSASSSPGSAFNEDATKDTDSLSSCTSDDSCVSSTLSTDMLTSSYKEPNNNFGVTLPCSSSSLCLDDVRLCLESGLQVQPGKKLSSLSNKHYKAKSAGTRRHSVPGTPTRGSPRRQRANACGKSSGGQTQAGAPEEDDDDGLPSLDTEVWSWGRGSEGQLGHGDQLARLQPLCIKTLSGEEVIQVAAGSHHSLAVTATCQVYSWGSNMCGQLGHDNSSFVVPQQAKLSDGLRVWDMSAGQSHSLLLADGDCVQPVLLYCGRQKESTSDQREKSYQRSPRKAEHYTVRPTLLPVCLEMGYISSVCSGGQSCAVLADQNIKVFISALHELASRERQFYCWLSRVRKLVLTPIQNKESTSPFLGDPCTHLFSSLCGSFSQLSVLIGRHSTSLTYFLQKAQGRDVTSLPLLTHMEQFLDTYKEYCSSVGDFQVMGGFQTLHKLTLECLQPEVLTQLCMSDQTSDDTGLVSLLYWPLQQLHQYDRVMVKVAFCFDVLMPEFQSFRQGCGQYESLSLSLLRKKKEAESTFLFWKSHSGKTTESLRLPRRRVVCESSSRSLTLQKAGRFSNHWFILFNDALVHTQGALPSQLFFSTHHVYPLTCLWVKPVTDDSSGLYAIKIICPEESFTMVASTPQEKNKWLRSINHALDQVLGGSGEGSSPGTMGMSRTASYTFTGEGRFKDAKYSGSWMSGRVHGRGSLRWPDGRTYCGNFKNGLEDGYGECVIPNKELNKPDSYHGHWREGKIHGFGKYKYASGEVYEGCFSEGQRQGYGMLSSGKLGKKSSSVFIGHWVHDKKTGYGVFDDITRGEKYMGLWVDEKRHGSGVVVTQYGVYYEGTFRDNKMSGPGLLMSDDDTAIHGEFSDDWVVNGKGVLLLANGDCLEGLFSGEWTSGLKVDGTYTKPLVDEPVNKERKNMFKLGRYVVPAGQRWVCVFEECWSRLGCDSTGRGDKNVAWENIAVSITAARRQSPDLSRSQNKVLESLEFIPQHEQPVTTSNYDSIRRYLIKACETPLHPLGWLVETLVTVYRMTYVGVEANRRLLEQAVQELQAYLTHLYNIVQFLFPGMPDDGAVIPDPPGSPSVVQNSSSMEDLSAVVVSCSSLLLPLLLPRLYPPLFTLYRLQEEQEDALYWDRILRLNKQPDQSLLSFLGVQEKFWPTWMSILGEKKQIVSSSKDACFVSAVETLQQISTTFTPSDKLVVIQKTFEELTQEVNPMLDGHFLWCMDDLLPLFIYVVVRARIRNLGAEVDMIEDLMDPNVQHGELGLMFTTLKACYIQIKNESTT

Target ORF information:

RefSeq Version	XM_011611411.1
Organism	Takifugu rubripes(Fugu rubripes)
Definition	Takifugu rubripes alsin-like (LOC101078293), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011611411.1

ORF Insert Sequence:

ATGTTATTCA GCTTGGCCCT GGTTCGTAGT TTGCCTCCAA ATAGCTACAA CAGCCAGAAG 
CCTTCTGAAA AAAGGGAACG GGGTCACTCG CCGCACTATT CAGCAGCAGA GAGAGAGGAG 
CTGTTTGTGG GTGACAGCAG TCTCTACTGT CCACTTGGGG TGGAGCTGAA AGAAGGCATG 
GCCACAGAGA CTTCCTCCAG GAGATGTCCA AGGAGAAGGC TCCATCCTGG AGGAAGTTCG 
GCCAGCAGCA GCCCTGGCTC TGCATTTAAC GAAGACGCGA CCAAAGACAC TGACTCACTG 
AGCAGCTGCA CCTCAGATGA CAGCTGTGTT TCCTCAACTC TCTCCACTGA TATGCTGACC 
TCCAGTTACA AAGAACCTAA CAATAACTTT GGAGTGACCC TGCCGTGCTC GTCCTCCTCA 
CTGTGTCTCG ACGATGTCCG ACTCTGTCTT GAATCGGGGC TGCAGGTACA GCCAGGGAAG 
AAGCTCTCCA GCCTCTCCAA TAAACACTAT AAAGCAAAAT CAGCAGGAAC CAGAAGACAT 
TCTGTCCCTG GGACACCCAC TCGTGGGTCT CCACGGCGAC AGCGTGCAAA CGCGTGCGGA 
AAATCTTCTG GTGGTCAGAC CCAGGCGGGA GCGCCGGAGG AGGATGATGA TGATGGGCTG 
CCATCTCTAG ACACAGAAGT CTGGAGCTGG GGACGGGGCT CTGAGGGGCA GCTCGGCCAC 
GGAGATCAGC TCGCCAGACT TCAGCCTCTG TGTATCAAGA CACTGTCGGG TGAGGAGGTG 
ATCCAAGTTG CAGCAGGGTC CCATCATTCT CTGGCTGTCA CTGCAACCTG TCAGGTGTAC 
TCATGGGGCA GCAACATGTG TGGGCAGCTG GGTCACGATA ACAGTTCTTT CGTTGTGCCC 
CAACAGGCAA AGCTGTCTGA TGGCCTCCGA GTTTGGGACA TGTCTGCCGG CCAGAGCCAC 
TCCCTCCTCC TGGCTGATGG AGACTGCGTC CAGCCCGTGT TGTTGTACTG TGGCCGGCAG 
AAAGAGTCGA CATCAGACCA GAGAGAAAAG TCCTACCAGA GGTCCCCCAG AAAAGCAGAG 
CATTACACAG TCAGGCCCAC CCTGCTGCCT GTCTGCCTGG AGATGGGCTA CATCAGCAGT 
GTGTGCAGCG GCGGGCAAAG CTGCGCCGTC CTGGCAGACC AGAACATCAA GGTTTTTATT 
TCGGCCCTCC ACGAGCTGGC CTCCAGAGAG AGGCAGTTCT ACTGCTGGTT GAGTCGCGTC 
AGGAAACTCG TCCTCACACC AATACAGAAC AAAGAGAGTA CGTCTCCGTT TTTAGGTGAC 
CCTTGCACCC ATCTCTTTTC TTCTCTCTGT GGGAGTTTTT CTCAGCTAAG CGTCCTAATC 
GGTCGACACT CCACATCGCT TACCTACTTC CTGCAAAAGG CACAGGGCCG TGACGTCACC 
TCACTCCCCC TGCTCACACA CATGGAGCAG TTTCTGGACA CTTACAAAGA ATATTGTTCC 
TCTGTTGGTG ACTTTCAGGT GATGGGGGGC TTCCAGACCC TCCATAAGCT CACACTTGAG 
TGTTTGCAGC CAGAAGTTCT CACTCAGCTG TGTATGTCGG ACCAGACCAG TGATGACACT 
GGTCTGGTTT CGCTGTTGTA CTGGCCTCTG CAGCAGCTCC ACCAGTACGA CCGAGTCATG 
GTCAAAGTGG CGTTTTGCTT CGACGTGTTA ATGCCAGAGT TTCAGTCCTT CCGTCAGGGC 
TGCGGTCAGT ACGAGTCCCT GTCTCTGTCC CTGTTGAGGA AGAAAAAAGA GGCTGAAAGC 
ACATTCCTCT TCTGGAAGAG CCACTCCGGA AAAACTACTG AGAGTCTACG TCTCCCTCGG 
CGCCGAGTGG TGTGTGAAAG CAGCAGCAGA TCTTTGACTC TGCAAAAGGC CGGGCGCTTC 
TCCAACCACT GGTTCATTCT GTTCAATGAT GCACTGGTCC ACACGCAGGG CGCGCTTCCC 
TCACAGCTCT TTTTCTCCAC ACATCACGTC TACCCCCTGA CCTGCCTGTG GGTGAAACCC 
GTCACTGACG ACAGCAGCGG ACTCTATGCC ATTAAAATTA TTTGCCCGGA GGAAAGTTTC 
ACCATGGTGG CTTCAACACC TCAAGAAAAG AACAAGTGGT TACGATCTAT CAATCACGCG 
CTGGATCAGG TGCTCGGTGG TAGTGGTGAG GGGTCGTCGC CGGGGACGAT GGGAATGTCC 
CGCACGGCCT CCTACACATT CACCGGAGAA GGCCGGTTTA AAGATGCCAA ATACAGCGGC 
AGCTGGATGT CAGGACGGGT GCATGGCAGA GGGTCCTTGA GGTGGCCTGA TGGGCGGACC 
TACTGCGGGA ACTTCAAGAA CGGCCTTGAA GATGGTTACG GGGAGTGCGT AATACCCAAC 
AAAGAGCTGA ACAAGCCTGA CAGTTACCAT GGACACTGGA GAGAGGGGAA GATCCACGGT 
TTCGGCAAAT ACAAGTACGC CAGCGGCGAG GTGTATGAAG GCTGTTTCAG TGAAGGGCAG 
CGGCAGGGTT ACGGCATGCT GAGCTCCGGG AAGCTGGGGA AGAAGTCCTC CAGTGTTTTC 
ATCGGCCACT GGGTCCATGA CAAGAAGACA GGTTATGGAG TGTTTGACGA CATCACCAGG 
GGTGAGAAGT ACATGGGATT GTGGGTAGAC GAGAAGCGGC ACGGCAGCGG CGTGGTGGTC 
ACACAGTATG GCGTGTACTA CGAAGGAACC TTCAGAGACA ACAAGATGAG TGGTCCCGGT 
CTGCTGATGT CGGATGACGA CACGGCTATT CATGGGGAGT TTTCCGACGA TTGGGTTGTC 
AACGGGAAGG GTGTTTTATT GTTGGCCAAC GGAGACTGTC TGGAGGGTCT GTTCAGTGGG 
GAGTGGACAT CTGGACTGAA GGTGGATGGA ACGTACACCA AACCACTGGT GGACGAGCCT 
GTAAACAAAG AAAGAAAAAA CATGTTCAAG CTGGGTAGGT ACGTGGTGCC TGCGGGTCAG 
AGGTGGGTCT GTGTGTTTGA GGAGTGCTGG AGTCGACTGG GCTGCGACAG CACTGGGAGA 
GGAGACAAAA ATGTAGCCTG GGAGAACATC GCAGTGTCCA TAACGGCTGC ACGGAGGCAA 
AGTCCAGATC TCAGTCGGTC TCAGAACAAA GTGTTGGAGT CTCTGGAGTT TATCCCTCAG 
CATGAGCAGC CAGTCACCAC CTCGAACTAC GACAGCATAC GCAGATACCT CATCAAGGCT 
TGTGAGACCC CCCTCCACCC TCTGGGCTGG CTGGTGGAGA CTCTGGTGAC GGTGTACCGG 
ATGACGTATG TGGGCGTGGA AGCTAACCGG AGGCTGCTGG AACAGGCTGT CCAGGAGCTT 
CAGGCCTACC TCACACATCT CTACAACATT GTCCAGTTTT TGTTTCCAGG GATGCCAGAT 
GATGGCGCCG TCATCCCAGA CCCCCCGGGG TCTCCATCGG TAGTCCAGAA CAGCTCCAGC 
ATGGAAGACT TGAGTGCTGT AGTGGTGAGC TGCTCCTCTC TGCTCCTCCC TCTGCTCCTG 
CCTCGACTCT ACCCTCCTCT CTTCACGCTG TACCGGCTGC AGGAGGAGCA GGAAGACGCA 
CTGTACTGGG ACCGCATCCT GCGTCTGAAC AAACAACCCG ATCAGTCGCT GCTGAGCTTC 
CTGGGAGTGC AAGAGAAGTT CTGGCCTACG TGGATGTCAA TTTTGGGGGA GAAGAAGCAG 
ATTGTGTCGA GCAGCAAAGA CGCCTGCTTC GTTTCCGCAG TTGAGACCCT CCAACAAATC 
AGCACCACCT TTACTCCATC TGACAAACTC GTCGTCATCC AGAAGACCTT TGAGGAGTTG 
ACCCAGGAAG TCAATCCCAT GCTGGACGGC CACTTCCTGT GGTGCATGGA TGACTTGCTC 
CCACTCTTCA TCTACGTGGT GGTCAGAGCG AGGATCAGGA ATCTGGGAGC GGAGGTCGAT 
ATGATTGAAG ATTTGATGGA TCCGAATGTT CAGCATGGAG AGCTGGGACT GATGTTCACC 
ACCCTGAAGG CCTGCTACAT CCAGATCAAA AACGAGTCTA CGACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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見積依頼

Eメール

[email protected]

電話

03-6811-6572

FAX

03-6811-6573
050-3174-7589(受注専用)

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オンライン注文

LOC101078293 cDNA ORF clone, Takifugu rubripes(torafugu)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム