LOC101069650 cDNA ORF clone, Takifugu rubripes(Fugu rubripes)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC101069650
Entrez Gene ID	101069650
Full Name	PH and SEC7 domain-containing protein 1-like
Gene Type	protein-coding
Organism	Takifugu rubripes(Fugu rubripes)

mRNA and Protein(s)

mRNA	Protein	Name
XM_029843696.1	XP_029699556.1	PH and SEC7 domain-containing protein 1-like
XM_011613580.1	XP_011611882.1	PH and SEC7 domain-containing protein 1-like

Pathways from BioSystems
Gene Ontology

The following LOC101069650 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC101069650 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OTo42298	XM_029843696.1 Latest version!	Takifugu rubripes PH and SEC7 domain-containing protein 1-like (LOC101069650), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OTo24892	XM_011613580.1 Latest version!	Takifugu rubripes PH and SEC7 domain-containing protein 1-like (LOC101069650), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC101069650 ( XM_029843696.1 ) cDNA ORF clone, Takifugu rubripes(Fugu rubripes) -> XP_029699556.1 Takifugu rubripes PH and SEC7 domain-containing protein 1-like (LOC101069650), mRNA.

Price & Availability↑

CloneID	OTo42298
Clone ID Related Accession (Same CDS sequence)	XM_029843696.1
Accession Version	XM_029843696.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3123bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-04
Organism	Takifugu rubripes(torafugu)
Product	PH and SEC7 domain-containing protein 1-like
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042285.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 14% of CDS bases ##RefSeq-Attributes-END##

ATGAGAGACC CCAGGCAGTT CCAAGCAGAG TTCCGAGCAC CAGACACACC CACTTTGCAC 
CGCCACCAGT TTCTCCCGTA CACCAGAGAG CCCTGGTCCC CCAGCCGGGA CAAGAGGGGA 
GACCTGAGCT GTTTTGAGAT TGGGAGTAGT CCAGAGCCCG ATCGCGGTCT TCTTCTCAGT 
CGGAGCATTG AGGAGGCGCC GGTTAGCTGG ACGTCACGGC AGCAGTGGGG GAACCTGACG 
GAGGAGGGCC CCGGGTCTGG AGTGGAGAAC GGCATCGATC AAACCTCCGA CAGGCGATCC 
GATGATGGAT GCAAGCAGCT CAGTCCCACA TCACAGCACA AACGGGCCGA GCAGAGGCGG 
AGGGAGATTC TGCTCCTGGG ACCGGTGGCT CTGGACTCTC ACGAGGAGGA CGAGGGTTTC 
AGTCCAGTCA CCACAGCAAC GAGACTGGGG TGGCCTCCTC AGGAATACAG ATGGACAGCG 
GCTGTGCGGC CCCCACGCCT TCGCTCCACT GTCAGAGAAT CGCCAGAGCC AAGTGGGAGT 
TTTTATTCGG GACCCCCGCT GAGGACGGCG CATGCAGGGG GGAGAAAGAT TCTCTTGAAG 
TCTCCACGGC TCCTCCGAGT GGGACGTCCA GCGAGTCTCC CACCCCGACC CCCCCCCTCC 
TCCCTGCCCT TGCTCTCTGC CAACCATGAG GTGCAGCACA TCGAGTTAGA ACTGGTCACC 
CCTCCTCCAG CCATCATCGG GACCTCGCCC AAAACCGGCA TTATCCGGCG CACCCTGAAG 
TACTCAGAAA CCGACTTGGA CGCCGTGCCG CTGCGCTGCT ACCGCGAGAC GGACATCGAC 
GAGGTGCTGC TGGCCGAACA GGACGACGCC GACTCAGCAT TTGGGAGCAA CCGCAGCGTG 
CAGGGCACCT CAGGGACGGG CAGCAGCCCG CTGGGAGGTG TGGCCGATGG GGGGGCGGCA 
GAGGGGATCC AAGAGGCCAG TGAAGAGGAG GAGGAAGAGG AAGAGATGGT GAGCTGGGCC 
AGCGTCAGGT TGCAGGGAGA CAACAGGAAG CAGCAGTACG CTGCCGAAGG AGACCCAGAG 
GAGTTTGGCC GCCTGATGAA GAGACCGACG GAAACCTTTT TCGACAACCA TCACCCCGCC 
CTCAAGTCCC CCCTCCTGGT CTCGGGTCCT CGCTGCGGTG CAGAGGACAC CTTCAGCCGC 
CACTTTGAGA ACATCATGGA ATCCCACAGG GCCAAGGGTA CGTCATACAA CAGCCTGGAC 
AGCGAGGAGC TGCTGACCTC CAGCACTCAG ACCGTCCTGA CCTTTGACCT CCCCACACTG 
ACCCCGACCA TCCACGCTCC GGTCTGTCAG ACCGCCCGGC AGATCGTCCA GCTCAGTTTT 
GCCCCTCTAG CACACGATGA GCGTCCCTCT CTGTCCGATT CCATCTTCAC CGTGACCGGA 
GACTCGGACG CCACCCGGGC GCCGTCCAGC GAGCGGCTGA GCAGCGGTTC GGACGACGTG 
CCCCCAGGAG GACACGGCCG TGCACAGCTG GGCAGCAGCT GGTGCAGCAC CTTTTCCTTC 
TCTTTGTCCA GCAGGGAAAG GGCCCGGCTG GCTTCGGACC CAGAGGTGGA CCGACTGGGT 
CTGAGCAGCA GTGACACCCT GACCAATGGA AGCCGCCATG CCGATGTGGC GGCTGCCAAA 
CGCTTGGCTA AACGCCTCTT CAACCTGGAT GGATTCAGGA AATCGGATGT CGCCCCCCAC 
CTCAGCAAAA ACAATGACTT CAGTCGCATG GTGGCAGAAG AATATCTGAG TTTCTTCAAC 
TTCACGGGCC TCAGTCTGGA CCAAGCTCTG CGGACGCTCC TCGGGCAGTT TGCACTGATG 
GGGGAGACAC AGGAGAGGGA GAGAGTCCTG TCACACTTCT CCAAGCGTTA CCTGTCCTGC 
AACCCAAGAG TGATACCATC GGAGGACGCC GTCCACACGC TCACCTGCGC CCTCATGCTG 
CTGAACACGG ATCTACACGG TCAGAACATC GGGAAAAGGA TGTCGTGTGC ACAGTTCGTC 
GGAAACCTGG AAGGACTGAA CGACGGCCAG GATTTTCACA AGGATCTGCT CAAAGCGCTG 
TACAACTCCA TCAAGAGCCA GAAACTGCAG TGGACACTCG ATGAGGAAGA GCTGCGGAAG 
TCTTTCTCGG AGCTCGGGGA CAGCCTGTGC GACTCCAACG CCTCCAAGTC CACCAAGCAG 
GTGGGCAGCG ACGAGAAGCC CCTGCCTGTC GGGTCAGAGC CCTCCGGAAC GCTGCTGTAC 
AAAAATGGAT TCCTGGTCCG CAAAGTTCAC GCTGACTCAG ACGGCAAGAG AACACCAAGA 
GGGAAGAGGG GCTGGAAAAC CTTCTATGCT ATCCTAAAAG GGCTAATTCT TTATCTGCAA 
AAGGGTGAAT ACAGACCTGA TAAGCAGCTG TCGGACGAGG ACCTGAAGAA CGCCGTGTCC 
ATCCATCACT CTCTGGCCAT GAAGGCTTCG GACTACAGCA AGAGGCCCAA CGTGTTCTAC 
CTGCGGACGG CCGACTGGAG GGTGTATCTC TTCCAGGCAC CGAACGCCGA GCAAATGCAG 
TCCTGGATCA CGCGGATCAA CACGGTGTCT GCCATGTTCT CGGCTCCTCC GTTCCCAGCG 
GCCATCGGTT CTCAGAAGAA GTTCAGCCGA CCTCTTCTTC CCGGGACCAT GTCGAAACTC 
TCCGAGGAGG AGCAGGTCCG ATCCCACGAA GCCCGATTCA AAGCTCTCTC CACGGAACTG 
GCTGAGCTGC GTTCCTATCC GCCGGACCGC AAGGTGAAAG GGCGCGAGCT GGAAGAGTAC 
CGCCTCCGAG ACGAGTATCT GGAGTTTGAG AAAACGCGAT ACGGCACCTA CGCCATGCTG 
CTGCGCGCTA AAATCCAGAG CGGCGCGGAG GACCTGGCTG CATTCGAGTC CCACCTCCTG 
GACGAAAACG GGCTGCAGAG GTGCCACTCG AGTCCCACGC TGCACGACAG CAGCCAGGTG 
AGCCAGGCCA GCAGCACGCT GGACGGCGGC AACTGCAGCA GAGCCAGCGG CTGCAGCAGC 
AGCAGCACCA AGGTCCGGCA GGAGGGCCAG AGACACAGCT ACCGGCAGGC CGTCAAAAAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029699556.1
CDS	1..3123
Translation	MRDPRQFQAEFRAPDTPTLHRHQFLPYTREPWSPSRDKRGDLSCFEIGSSPEPDRGLLLSRSIEEAPVSWTSRQQWGNLTEEGPGSGVENGIDQTSDRRSDDGCKQLSPTSQHKRAEQRRREILLLGPVALDSHEEDEGFSPVTTATRLGWPPQEYRWTAAVRPPRLRSTVRESPEPSGSFYSGPPLRTAHAGGRKILLKSPRLLRVGRPASLPPRPPPSSLPLLSANHEVQHIELELVTPPPAIIGTSPKTGIIRRTLKYSETDLDAVPLRCYRETDIDEVLLAEQDDADSAFGSNRSVQGTSGTGSSPLGGVADGGAAEGIQEASEEEEEEEEMVSWASVRLQGDNRKQQYAAEGDPEEFGRLMKRPTETFFDNHHPALKSPLLVSGPRCGAEDTFSRHFENIMESHRAKGTSYNSLDSEELLTSSTQTVLTFDLPTLTPTIHAPVCQTARQIVQLSFAPLAHDERPSLSDSIFTVTGDSDATRAPSSERLSSGSDDVPPGGHGRAQLGSSWCSTFSFSLSSRERARLASDPEVDRLGLSSSDTLTNGSRHADVAAAKRLAKRLFNLDGFRKSDVAPHLSKNNDFSRMVAEEYLSFFNFTGLSLDQALRTLLGQFALMGETQERERVLSHFSKRYLSCNPRVIPSEDAVHTLTCALMLLNTDLHGQNIGKRMSCAQFVGNLEGLNDGQDFHKDLLKALYNSIKSQKLQWTLDEEELRKSFSELGDSLCDSNASKSTKQVGSDEKPLPVGSEPSGTLLYKNGFLVRKVHADSDGKRTPRGKRGWKTFYAILKGLILYLQKGEYRPDKQLSDEDLKNAVSIHHSLAMKASDYSKRPNVFYLRTADWRVYLFQAPNAEQMQSWITRINTVSAMFSAPPFPAAIGSQKKFSRPLLPGTMSKLSEEEQVRSHEARFKALSTELAELRSYPPDRKVKGRELEEYRLRDEYLEFEKTRYGTYAMLLRAKIQSGAEDLAAFESHLLDENGLQRCHSSPTLHDSSQVSQASSTLDGGNCSRASGCSSSSTKVRQEGQRHSYRQAVKK

Target ORF information:

RefSeq Version	XM_029843696.1
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes PH and SEC7 domain-containing protein 1-like (LOC101069650), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029843696.1

ORF Insert Sequence:

ATGAGAGACC CCAGGCAGTT CCAAGCAGAG TTCCGAGCAC CAGACACACC CACTTTGCAC 
CGCCACCAGT TTCTCCCGTA CACCAGAGAG CCCTGGTCCC CCAGCCGGGA CAAGAGGGGA 
GACCTGAGCT GTTTTGAGAT TGGGAGTAGT CCAGAGCCCG ATCGCGGTCT TCTTCTCAGT 
CGGAGCATTG AGGAGGCGCC GGTTAGCTGG ACGTCACGGC AGCAGTGGGG GAACCTGACG 
GAGGAGGGCC CCGGGTCTGG AGTGGAGAAC GGCATCGATC AAACCTCCGA CAGGCGATCC 
GATGATGGAT GCAAGCAGCT CAGTCCCACA TCACAGCACA AACGGGCCGA GCAGAGGCGG 
AGGGAGATTC TGCTCCTGGG ACCGGTGGCT CTGGACTCTC ACGAGGAGGA CGAGGGTTTC 
AGTCCAGTCA CCACAGCAAC GAGACTGGGG TGGCCTCCTC AGGAATACAG ATGGACAGCG 
GCTGTGCGGC CCCCACGCCT TCGCTCCACT GTCAGAGAAT CGCCAGAGCC AAGTGGGAGT 
TTTTATTCGG GACCCCCGCT GAGGACGGCG CATGCAGGGG GGAGAAAGAT TCTCTTGAAG 
TCTCCACGGC TCCTCCGAGT GGGACGTCCA GCGAGTCTCC CACCCCGACC CCCCCCCTCC 
TCCCTGCCCT TGCTCTCTGC CAACCATGAG GTGCAGCACA TCGAGTTAGA ACTGGTCACC 
CCTCCTCCAG CCATCATCGG GACCTCGCCC AAAACCGGCA TTATCCGGCG CACCCTGAAG 
TACTCAGAAA CCGACTTGGA CGCCGTGCCG CTGCGCTGCT ACCGCGAGAC GGACATCGAC 
GAGGTGCTGC TGGCCGAACA GGACGACGCC GACTCAGCAT TTGGGAGCAA CCGCAGCGTG 
CAGGGCACCT CAGGGACGGG CAGCAGCCCG CTGGGAGGTG TGGCCGATGG GGGGGCGGCA 
GAGGGGATCC AAGAGGCCAG TGAAGAGGAG GAGGAAGAGG AAGAGATGGT GAGCTGGGCC 
AGCGTCAGGT TGCAGGGAGA CAACAGGAAG CAGCAGTACG CTGCCGAAGG AGACCCAGAG 
GAGTTTGGCC GCCTGATGAA GAGACCGACG GAAACCTTTT TCGACAACCA TCACCCCGCC 
CTCAAGTCCC CCCTCCTGGT CTCGGGTCCT CGCTGCGGTG CAGAGGACAC CTTCAGCCGC 
CACTTTGAGA ACATCATGGA ATCCCACAGG GCCAAGGGTA CGTCATACAA CAGCCTGGAC 
AGCGAGGAGC TGCTGACCTC CAGCACTCAG ACCGTCCTGA CCTTTGACCT CCCCACACTG 
ACCCCGACCA TCCACGCTCC GGTCTGTCAG ACCGCCCGGC AGATCGTCCA GCTCAGTTTT 
GCCCCTCTAG CACACGATGA GCGTCCCTCT CTGTCCGATT CCATCTTCAC CGTGACCGGA 
GACTCGGACG CCACCCGGGC GCCGTCCAGC GAGCGGCTGA GCAGCGGTTC GGACGACGTG 
CCCCCAGGAG GACACGGCCG TGCACAGCTG GGCAGCAGCT GGTGCAGCAC CTTTTCCTTC 
TCTTTGTCCA GCAGGGAAAG GGCCCGGCTG GCTTCGGACC CAGAGGTGGA CCGACTGGGT 
CTGAGCAGCA GTGACACCCT GACCAATGGA AGCCGCCATG CCGATGTGGC GGCTGCCAAA 
CGCTTGGCTA AACGCCTCTT CAACCTGGAT GGATTCAGGA AATCGGATGT CGCCCCCCAC 
CTCAGCAAAA ACAATGACTT CAGTCGCATG GTGGCAGAAG AATATCTGAG TTTCTTCAAC 
TTCACGGGCC TCAGTCTGGA CCAAGCTCTG CGGACGCTCC TCGGGCAGTT TGCACTGATG 
GGGGAGACAC AGGAGAGGGA GAGAGTCCTG TCACACTTCT CCAAGCGTTA CCTGTCCTGC 
AACCCAAGAG TGATACCATC GGAGGACGCC GTCCACACGC TCACCTGCGC CCTCATGCTG 
CTGAACACGG ATCTACACGG TCAGAACATC GGGAAAAGGA TGTCGTGTGC ACAGTTCGTC 
GGAAACCTGG AAGGACTGAA CGACGGCCAG GATTTTCACA AGGATCTGCT CAAAGCGCTG 
TACAACTCCA TCAAGAGCCA GAAACTGCAG TGGACACTCG ATGAGGAAGA GCTGCGGAAG 
TCTTTCTCGG AGCTCGGGGA CAGCCTGTGC GACTCCAACG CCTCCAAGTC CACCAAGCAG 
GTGGGCAGCG ACGAGAAGCC CCTGCCTGTC GGGTCAGAGC CCTCCGGAAC GCTGCTGTAC 
AAAAATGGAT TCCTGGTCCG CAAAGTTCAC GCTGACTCAG ACGGCAAGAG AACACCAAGA 
GGGAAGAGGG GCTGGAAAAC CTTCTATGCT ATCCTAAAAG GGCTAATTCT TTATCTGCAA 
AAGGGTGAAT ACAGACCTGA TAAGCAGCTG TCGGACGAGG ACCTGAAGAA CGCCGTGTCC 
ATCCATCACT CTCTGGCCAT GAAGGCTTCG GACTACAGCA AGAGGCCCAA CGTGTTCTAC 
CTGCGGACGG CCGACTGGAG GGTGTATCTC TTCCAGGCAC CGAACGCCGA GCAAATGCAG 
TCCTGGATCA CGCGGATCAA CACGGTGTCT GCCATGTTCT CGGCTCCTCC GTTCCCAGCG 
GCCATCGGTT CTCAGAAGAA GTTCAGCCGA CCTCTTCTTC CCGGGACCAT GTCGAAACTC 
TCCGAGGAGG AGCAGGTCCG ATCCCACGAA GCCCGATTCA AAGCTCTCTC CACGGAACTG 
GCTGAGCTGC GTTCCTATCC GCCGGACCGC AAGGTGAAAG GGCGCGAGCT GGAAGAGTAC 
CGCCTCCGAG ACGAGTATCT GGAGTTTGAG AAAACGCGAT ACGGCACCTA CGCCATGCTG 
CTGCGCGCTA AAATCCAGAG CGGCGCGGAG GACCTGGCTG CATTCGAGTC CCACCTCCTG 
GACGAAAACG GGCTGCAGAG GTGCCACTCG AGTCCCACGC TGCACGACAG CAGCCAGGTG 
AGCCAGGCCA GCAGCACGCT GGACGGCGGC AACTGCAGCA GAGCCAGCGG CTGCAGCAGC 
AGCAGCACCA AGGTCCGGCA GGAGGGCCAG AGACACAGCT ACCGGCAGGC CGTCAAAAAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101069650 ( XM_011613580.1 ) cDNA ORF clone, Takifugu rubripes(Fugu rubripes) -> XP_011611882.1 Takifugu rubripes PH and SEC7 domain-containing protein 1-like (LOC101069650), mRNA.

Price & Availability↑

CloneID	OTo24892
Clone ID Related Accession (Same CDS sequence)	XM_011613580.1
Accession Version	XM_011613580.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2286bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-15
Organism	Takifugu rubripes(Fugu rubripes)
Product	PH and SEC7 domain-containing protein 1-like
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004070898.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Takifugu rubripes Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 2% of CDS bases ##RefSeq-Attributes-END##

ATGGTGAGCT GGGCCAGCGT CAGGTTGCAG GGAGACAACA GGAAGCAGCA GTACGCTGCC 
GAAGGAGACC CAGAGGAGTT TGGCCGCCTG ATGAAGAGAC CAACGGAAAC CTTTTTCGAC 
AACCATCACC CCGCCCTCAA GTCCCCCCTC CTGGTCTCGG GTCCTCGCTG CGGTGCAGAG 
GACACCTTCA GCCGCCACTT TGAGAACATC ATGGAATCCC ACAGGGCCAA GGGTACGTCA 
TACAACAGCC TGGACAGCGA GGAGCTGCTG ACCTCCAGCA CTCAGACCGT CCTGACCTTT 
GACCTCCCCA CACTGACCCC GACCATCCAC GCTCCGGTCT GTCAGACCGC CCGGCAGATC 
GTCCAGCTCA GTTTTGCCCC TCTAGCACAC GATGAGCGTC CCTCTCTGTC CGATTCCATC 
TTCACCGTGA CCGGAGACTC GGACGCCACC CGGGCGCCGT CCAGCGAGCG GCTGAGCAGC 
GGTTCGGACG ACGTGCCCCC AGGAGGACAC GGCCGTGCAC AGCTGGGCAG CAGCTGGTGC 
AGCACCTTTT CCTTCTCTTT GTCCAGACGG GACGCGGCCG TGATGCATTC AGGTGTCCGG 
CCCGGCAGGA GAGGAGGGGC CGGCCGGGCG AGCGGCCGAG GTCCCGGCCG GGGGGCAGCG 
TCGAGGTCAA GACCGCTCTT CTGTTGCAAA GGTTCATCTG CGAAATACTC GCCGATCAAA 
CGGTCTCCTC ACAGCAGGGA AAGGGCCCGG CTGGCTTCGG ACCCAGAGGT GGACCGACTG 
GGTCTGAGCA GCAGTGACAC CCTGACCAAT GGAAGCCGCC ATGCCGATGT GGCGGCTGCC 
AAACGCTTGG CTAAACGCCT CTTCAACCTG GATGGATTCA GGAAATCGGA TGTCGCCCCC 
CACCTCAGCA AAAACAATGA CTTCAGTCGC ATGGTGGCAG AAGAATATCT GAGTTTCTTC 
AACTTCACGG GCCTCAGTCT GGACCAAGCT CTGCGGACGC TCCTCGGGCA GTTTGCACTG 
ATGGGGGAGA CACAGGAGAG GGAGAGAGTC CTGTCACACT TCTCCAAGCG TTACCTGTCC 
TGCAACCCAA GAGTGATACC ATCGGAGGAC GCCGTCCACA CGCTCACCTG CGCCCTCATG 
CTGCTGAACA CGGATCTACA CGGTCAGAAC ATCGGGAAAA GGATGTCGTG TGCACAGTTC 
GTCGGAAACC TGGAAGGACT GAACGACGGC CAGGATTTTC ACAAGGATCT GCTCAAAGCG 
CTGTACAACT CCATCAAGAG CCAGAAGCTG CAGTGGACAC TCGATGAGGA AGAGCTGCGG 
AAGTCTTTCT CGGAGCTCGG GGACAGCCTG TGCGACTCCA ACGCCTCCAA GTCCACCAAG 
CAGGTGGGCA GCGACGAGAA GCCCCTGCCT GTCGGGTCAG AGCCCTCTGG AACGCTGCTG 
TACAAAAATG GATTCCTGGT CCGCAAAGTT CACGCTGACT CAGACGGCAA GAGAACACCA 
AGAGGGAAGA GGGGCTGGAA AACCTTCTAT GCTATCCTAA AAGGGCTAAT TCTTTATCTG 
CAAAAGGGTG AATACAGACC TGATAAGCAG CTGTCGGACG AGGACCTGAA GAACGCCGTG 
TCCATCCATC ACTCTCTGGC CATGAAGGCT TCGGACTACA GCAAGAGGCC CAACGTGTTC 
TACCTGCGGA CGGCCGACTG GAGGGTGTAT CTCTTCCAGG CACCGAACGC CGAGCAAATG 
CAGTCCTGGA TCACGCGGAT CAACACGGTG TCTGCCATGT TCTCGGCTCC TCCGTTCCCA 
GCGGCCATCG GTTCTCAGAA GAAGTTCAGC CGACCTCTTC TTCCCGGGAC CATGTCGAAA 
CTCTCCGAGG AGGAGCAGGT CCGATCCCAC GAAGCCCGAT TCAAAGCTCT CTCCACGGAA 
CTGGCTGAGC TGCGTTCCTA TCCGCCGGAC CGCAAGGTGA AAGGGCGCGA GCTGGAAGAG 
TACCGCCTCC GAGACGAGTA TCTGGAGTTT GAGAAAACGC GATACGGCAC CTACGCCATG 
CTGCTGCGCG CTAAAATCCA GAGCGGCGCG GAGGACCTGG CTGCATTCGA GTCCCACCTC 
CTGGACGAAA ACGGGCTGCA GAGGTGCCAC TCGAGTCCCA CGCTGCACGA CAGCAGCCAG 
GTGAGCCAGG CCAGCAGCAC GCTGGACGGC GGCAACTGCA GCAGAGCCAG CGGCTGCAGC 
AGCAGCAGCA CCAAGGTCCG GCAGGAGGGC CAGAGACACA GCTACCGGCA GGCCGTCAAA 
AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011611882.1
CDS	120..2405
Translation	MVSWASVRLQGDNRKQQYAAEGDPEEFGRLMKRPTETFFDNHHPALKSPLLVSGPRCGAEDTFSRHFENIMESHRAKGTSYNSLDSEELLTSSTQTVLTFDLPTLTPTIHAPVCQTARQIVQLSFAPLAHDERPSLSDSIFTVTGDSDATRAPSSERLSSGSDDVPPGGHGRAQLGSSWCSTFSFSLSRRDAAVMHSGVRPGRRGGAGRASGRGPGRGAASRSRPLFCCKGSSAKYSPIKRSPHSRERARLASDPEVDRLGLSSSDTLTNGSRHADVAAAKRLAKRLFNLDGFRKSDVAPHLSKNNDFSRMVAEEYLSFFNFTGLSLDQALRTLLGQFALMGETQERERVLSHFSKRYLSCNPRVIPSEDAVHTLTCALMLLNTDLHGQNIGKRMSCAQFVGNLEGLNDGQDFHKDLLKALYNSIKSQKLQWTLDEEELRKSFSELGDSLCDSNASKSTKQVGSDEKPLPVGSEPSGTLLYKNGFLVRKVHADSDGKRTPRGKRGWKTFYAILKGLILYLQKGEYRPDKQLSDEDLKNAVSIHHSLAMKASDYSKRPNVFYLRTADWRVYLFQAPNAEQMQSWITRINTVSAMFSAPPFPAAIGSQKKFSRPLLPGTMSKLSEEEQVRSHEARFKALSTELAELRSYPPDRKVKGRELEEYRLRDEYLEFEKTRYGTYAMLLRAKIQSGAEDLAAFESHLLDENGLQRCHSSPTLHDSSQVSQASSTLDGGNCSRASGCSSSSTKVRQEGQRHSYRQAVKK

Target ORF information:

RefSeq Version	XM_011613580.1
Organism	Takifugu rubripes(Fugu rubripes)
Definition	Takifugu rubripes PH and SEC7 domain-containing protein 1-like (LOC101069650), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011613580.1

ORF Insert Sequence:

ATGGTGAGCT GGGCCAGCGT CAGGTTGCAG GGAGACAACA GGAAGCAGCA GTACGCTGCC 
GAAGGAGACC CAGAGGAGTT TGGCCGCCTG ATGAAGAGAC CAACGGAAAC CTTTTTCGAC 
AACCATCACC CCGCCCTCAA GTCCCCCCTC CTGGTCTCGG GTCCTCGCTG CGGTGCAGAG 
GACACCTTCA GCCGCCACTT TGAGAACATC ATGGAATCCC ACAGGGCCAA GGGTACGTCA 
TACAACAGCC TGGACAGCGA GGAGCTGCTG ACCTCCAGCA CTCAGACCGT CCTGACCTTT 
GACCTCCCCA CACTGACCCC GACCATCCAC GCTCCGGTCT GTCAGACCGC CCGGCAGATC 
GTCCAGCTCA GTTTTGCCCC TCTAGCACAC GATGAGCGTC CCTCTCTGTC CGATTCCATC 
TTCACCGTGA CCGGAGACTC GGACGCCACC CGGGCGCCGT CCAGCGAGCG GCTGAGCAGC 
GGTTCGGACG ACGTGCCCCC AGGAGGACAC GGCCGTGCAC AGCTGGGCAG CAGCTGGTGC 
AGCACCTTTT CCTTCTCTTT GTCCAGACGG GACGCGGCCG TGATGCATTC AGGTGTCCGG 
CCCGGCAGGA GAGGAGGGGC CGGCCGGGCG AGCGGCCGAG GTCCCGGCCG GGGGGCAGCG 
TCGAGGTCAA GACCGCTCTT CTGTTGCAAA GGTTCATCTG CGAAATACTC GCCGATCAAA 
CGGTCTCCTC ACAGCAGGGA AAGGGCCCGG CTGGCTTCGG ACCCAGAGGT GGACCGACTG 
GGTCTGAGCA GCAGTGACAC CCTGACCAAT GGAAGCCGCC ATGCCGATGT GGCGGCTGCC 
AAACGCTTGG CTAAACGCCT CTTCAACCTG GATGGATTCA GGAAATCGGA TGTCGCCCCC 
CACCTCAGCA AAAACAATGA CTTCAGTCGC ATGGTGGCAG AAGAATATCT GAGTTTCTTC 
AACTTCACGG GCCTCAGTCT GGACCAAGCT CTGCGGACGC TCCTCGGGCA GTTTGCACTG 
ATGGGGGAGA CACAGGAGAG GGAGAGAGTC CTGTCACACT TCTCCAAGCG TTACCTGTCC 
TGCAACCCAA GAGTGATACC ATCGGAGGAC GCCGTCCACA CGCTCACCTG CGCCCTCATG 
CTGCTGAACA CGGATCTACA CGGTCAGAAC ATCGGGAAAA GGATGTCGTG TGCACAGTTC 
GTCGGAAACC TGGAAGGACT GAACGACGGC CAGGATTTTC ACAAGGATCT GCTCAAAGCG 
CTGTACAACT CCATCAAGAG CCAGAAGCTG CAGTGGACAC TCGATGAGGA AGAGCTGCGG 
AAGTCTTTCT CGGAGCTCGG GGACAGCCTG TGCGACTCCA ACGCCTCCAA GTCCACCAAG 
CAGGTGGGCA GCGACGAGAA GCCCCTGCCT GTCGGGTCAG AGCCCTCTGG AACGCTGCTG 
TACAAAAATG GATTCCTGGT CCGCAAAGTT CACGCTGACT CAGACGGCAA GAGAACACCA 
AGAGGGAAGA GGGGCTGGAA AACCTTCTAT GCTATCCTAA AAGGGCTAAT TCTTTATCTG 
CAAAAGGGTG AATACAGACC TGATAAGCAG CTGTCGGACG AGGACCTGAA GAACGCCGTG 
TCCATCCATC ACTCTCTGGC CATGAAGGCT TCGGACTACA GCAAGAGGCC CAACGTGTTC 
TACCTGCGGA CGGCCGACTG GAGGGTGTAT CTCTTCCAGG CACCGAACGC CGAGCAAATG 
CAGTCCTGGA TCACGCGGAT CAACACGGTG TCTGCCATGT TCTCGGCTCC TCCGTTCCCA 
GCGGCCATCG GTTCTCAGAA GAAGTTCAGC CGACCTCTTC TTCCCGGGAC CATGTCGAAA 
CTCTCCGAGG AGGAGCAGGT CCGATCCCAC GAAGCCCGAT TCAAAGCTCT CTCCACGGAA 
CTGGCTGAGC TGCGTTCCTA TCCGCCGGAC CGCAAGGTGA AAGGGCGCGA GCTGGAAGAG 
TACCGCCTCC GAGACGAGTA TCTGGAGTTT GAGAAAACGC GATACGGCAC CTACGCCATG 
CTGCTGCGCG CTAAAATCCA GAGCGGCGCG GAGGACCTGG CTGCATTCGA GTCCCACCTC 
CTGGACGAAA ACGGGCTGCA GAGGTGCCAC TCGAGTCCCA CGCTGCACGA CAGCAGCCAG 
GTGAGCCAGG CCAGCAGCAC GCTGGACGGC GGCAACTGCA GCAGAGCCAG CGGCTGCAGC 
AGCAGCAGCA CCAAGGTCCG GCAGGAGGGC CAGAGACACA GCTACCGGCA GGCCGTCAAA 
AAGTGA