map3k10 cDNA ORF clone, Takifugu rubripes(torafugu)

Gene
Clones
gRNAs

Summary

Gene Symbol	map3k10
Entrez Gene ID	101068242
Full Name	mitogen-activated protein kinase kinase kinase 10
Gene Type	protein-coding
Organism	Takifugu rubripes(torafugu)

mRNA and Protein(s)

mRNA	Protein	Name
XM_011608379.1	XP_011606681.1	mitogen-activated protein kinase kinase kinase 10
XM_011608379.2	XP_011606681.1	mitogen-activated protein kinase kinase kinase 10

Pathways from BioSystems
Gene Ontology

The following map3k10 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the map3k10 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OTo23942	XM_011608379.2 Latest version!	Takifugu rubripes mitogen-activated protein kinase kinase kinase 10 (map3k10), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
View Old Accession Versions >>
OTo23942	XM_011608379.1	Takifugu rubripes mitogen-activated protein kinase kinase kinase 10 (map3k10), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

map3k10 ( XM_011608379.1 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_011606681.1 Takifugu rubripes mitogen-activated protein kinase kinase kinase 10 (map3k10), mRNA.

Price & Availability↑

CloneID	OTo23942
Clone ID Related Accession (Same CDS sequence)	XM_011608379.1 , XM_011608379.2
Accession Version	XM_011608379.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3282bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-15
Organism	Takifugu rubripes(Fugu rubripes)
Product	mitogen-activated protein kinase kinase kinase 10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004071040.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Takifugu rubripes Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACCCGT ACGGCAGAGC AAGCAGCACG GCGCCCCCCC TGTCCTGCAC CAGCTGTGGG 
GGCTGCTGCT CGCCGCCTCC CCCTTGCTTA ATCCCACCAG GGCCCCCATC TACTACCTCC 
TCTTCCTCAA TGCCACCGAA TATTACCCCT CCGCCGCCGA TGTTCCCGGC CCCCGTGGTG 
CAGGTGGAGA ACGGCAGCAG CTGGAACTCT TCGTCCGGTT CCCCGGACTC CCACCCAGGT 
CGCCGCTGTG GGGTCACCAA CCCCAACCCT TACTGGACAG CAGTGTTTGA CTATGAGGCC 
ACAGCGGACG AGGAGTTAAC CCTGCGGCGC GGGGACCTCC TGGAGGTCCT CTCCAAAGAC 
TCAAAGGTGT CCGGGGATGA GGGTTGGTGG ACAGGCAAGA TCCAGGACAA GGTGGGCATC 
TTCCCCAGCA ACTATGTGAC CAAGGGGGAC GCGGCCAACT ACAAGCAGCT CGCTGCTGGA 
GGTCTGGTGG CGGGCGGGCT AGGCGAGTGT CCTCTGGAGA TAGACTTCCT AGAACTGCAC 
CTGGAAGAGG TGATCGGAGC GGGCGGGTTT GGGAAAGTGT ACAAAGGAAT GTGGAGGAAC 
GAAGAGGTAG CGGTGAAGGC CGCCAGGCAA GACCCCGACG AGGACATCAG CGCCACGGCG 
GAGAGCGTGC GCCAGGAGGC CAGGCTGTTC TGGATGCTCC AACATCGCAA CATAATCGCC 
CTGCGCGGGG TGTGCCTGCG CGAGCCCAAC CTGTGCTTGG TGATGGAGTA CGCCAGAGGG 
GGGGCGCTGA ACCGAGCACT CGCTGGAAAG AAGGTGCCCC CAAAGGTTCT GGTGAACTGG 
GCGGTCCAGA TCGCTGCGGG GATGGACTAC CTGCACAACC AGGCGTTCGT GCCCATCATT 
CACAGGGACC TCAAGTCCAG CAATATTCTC ATCCTGGAGC CTCTGGAGAG GCTCAGCTTG 
GGCAGTAAAA CCTTAAAGAT CACAGACTTC GGTCTGGCCA GGGAGTGGCA TCAGACCACC 
AAGATGAGCG CGGCGGGGAC GTACGCCTGG ATGGCCCCCG AGGTCATCAA ACTGTCCCTC 
TTCTCCAAAA GCAGCGACGT GTGGAGTTTT GGGGTGCTAC TGTGGGAGCT GCTGACCGGC 
GAGGTCCCCT ACCGAGAGAT CGACGCTCTG GCGGTAGCAT ACGGCGTCGC CATGAACAAA 
CTAACCCTCC CCATCCCGTC AACGTGCCCC GAGCCCTTCG CTCAGCTGCT GGAAGAGTGT 
TGGAGCCCAA ACCCCCACAG CAGACCCTCC TTCACCAGCA TCCTGAGGCA GCTGTTGGCC 
ATCGAGCAGT CGGCCATGTT TCAGATGCCT CTGGAGTCGT TCCACTCTTT ACAAGAGGAC 
TGGAGGCTGG AGATCCAGCA GATGTTTGAT GAACTCAGGG CCAAAGAGAA GGAACTAAGA 
TCATGGGAGG AGGCGCTGGC AAGGGCGGCC GAGGAGCAGC GGGAGCAGGA GGAGCAGCTG 
CGGAGGCGGG AGCAGGAGCT GGCGGAGAGG GAGATCGATA TCGTGGAACG AGAGCTGAAC 
ATCATCATCC ACCAGGTGTA CCAGGAGAAA CCCAGCGTGA AGAAAAGGAA AGGCCACTTT 
AAAAAGAGCA GACTGCTGAA GCTGGGCCGC GACAGCAACT GCATCAGTCT GCCATCTGGC 
TTCGAGCATA AGATCACCGT CCAGGCATCT CCCAAGGTGG ACAAGAGGAA GCCTCAGGGC 
AGCGAGAGCA CCACCCCACC CGCCAGTCCA GGGGTCATTC CCCGATTGAG AGCCATCAGA 
TTGACGCCGA CCGACGGCAG CAAGACGTGG GGGCGCAGCG CCGTGTGCAA GAAGGAGGAC 
CTGTCGAGCA GCAAGAAGAA AGGACGCACC TGGGGGCCCA GCTCCACGCA CCAGAAAGAA 
AGAGTTGGTG GAGAAGACAA GCTGAAGTCT CTGGGCGAGG GATGCAAAGT GTGGTCGTCC 
AGTGCTCCAA ATCTGGGCAA GTCCCCCAAA CATGCCCCCA TGACCGCCGG CTTCTCCAGC 
CTTAACGAAA TGGAGGAGTG CTGTGACCCG GAGGAGTCGC CCGGCTCCCT GCAGCCGTCG 
GAGACCAGCA GCAACGGCGC TGTGGACGAT TCGGGGTCTC TGTGGAGCAG CCCGTCGGCC 
ACTGGCGGCT CCTGCCAGGG CTCCGTGGGT CAGGGATCGG GGGCGAACTA CCAGGAGTCT 
GCGCGGCGCT GCAGCCAGAG GAAGAAAAGC GACATGTTGC TGCTGGGGTG TGCCTCTCTG 
CTGGCCTCCG TCGCCCTGGG ACAGGACCTC CTGCAGCTGG GAAAACTACA GGTCCTTCAG 
GATGAGCAGG ACCTCAGAGA CGAGCAGCGT AAGAAGAAAG ACGGCCTTTT CCAGAGGGCG 
GGGCGCTTCC GCCGCAGCAC CTCCCCGCCC AGCCGAAACC TCTCCCTCTC CCTCTCCCGA 
CACCATGAGT CCATGCTCCC CAGCACGGAC CCCTCGCCCT CTGTCACCCT CCTCTCCTTG 
TCCTCTCTGT CTGACTGCAA CTCCACCAAG TCCCTCCTTC CCTCTGACCC GGAGGAGTAC 
CCCCTGACCC CCATGATGGG GGCGAAAACC CCAGCGGCCC CGCCGGCTCA CGCCCTCAAC 
CCTCTGCTGG ACCTGCGGGC AGAGAGCTTT AAGAAGGAGC CCAACCAGTC TCTGACACCC 
ACCCACGTGT CTGCAGCCAT GGCCATGAAC AGAGGGCACA GACGCACCCC CTCAGACGGG 
GCCATACGGC CCCGGGCTCA GACTCTGGGA CATCGCAGGA CTCCGTCAGA CGGAAGCATG 
CCCATGCCCC CCCCACCTGG AGGACTGAAC AAACCTGCAA ACAAAGGGAC ACAGGAAAGC 
TTGGAAATCC CCCGCCTCCC CATCCCCACC TCCGTCTTCC CCGTCCCCCA CCGACGCAAA 
GCTCCCGCTC CGCCGGTCCC CAACCCCGAC GCCTTCTGCC TCATCAGCCC CCTGGAGAGG 
CCCAAAACGC TGGAATTCGC ACCCCGGCCT CGGCCCACGC CGGCCCGCGT GAGGCCGTCC 
GACGCCTGGA AGCTGAGCTC GCTGTCGCGC ACCCTCAGCT CGTCCCCGGG CAGCAGCTGT 
GACAGCCCCC AGGGTTTAGG GGACGGCGAG GACGGCGGCG GCGCTAGTGC GGCGGCGCAG 
CAAAACCTGA TGGACATGGA CGTGGAGGGT CAGAGCTTAG ACCAGACGGT CCCTCTGTGT 
GGACAGCAGC ACCCCACTGC GCTCTGTGGA CAACAACTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011606681.1
CDS	1759..5040
Translation	MNPYGRASSTAPPLSCTSCGGCCSPPPPCLIPPGPPSTTSSSSMPPNITPPPPMFPAPVVQVENGSSWNSSSGSPDSHPGRRCGVTNPNPYWTAVFDYEATADEELTLRRGDLLEVLSKDSKVSGDEGWWTGKIQDKVGIFPSNYVTKGDAANYKQLAAGGLVAGGLGECPLEIDFLELHLEEVIGAGGFGKVYKGMWRNEEVAVKAARQDPDEDISATAESVRQEARLFWMLQHRNIIALRGVCLREPNLCLVMEYARGGALNRALAGKKVPPKVLVNWAVQIAAGMDYLHNQAFVPIIHRDLKSSNILILEPLERLSLGSKTLKITDFGLAREWHQTTKMSAAGTYAWMAPEVIKLSLFSKSSDVWSFGVLLWELLTGEVPYREIDALAVAYGVAMNKLTLPIPSTCPEPFAQLLEECWSPNPHSRPSFTSILRQLLAIEQSAMFQMPLESFHSLQEDWRLEIQQMFDELRAKEKELRSWEEALARAAEEQREQEEQLRRREQELAEREIDIVERELNIIIHQVYQEKPSVKKRKGHFKKSRLLKLGRDSNCISLPSGFEHKITVQASPKVDKRKPQGSESTTPPASPGVIPRLRAIRLTPTDGSKTWGRSAVCKKEDLSSSKKKGRTWGPSSTHQKERVGGEDKLKSLGEGCKVWSSSAPNLGKSPKHAPMTAGFSSLNEMEECCDPEESPGSLQPSETSSNGAVDDSGSLWSSPSATGGSCQGSVGQGSGANYQESARRCSQRKKSDMLLLGCASLLASVALGQDLLQLGKLQVLQDEQDLRDEQRKKKDGLFQRAGRFRRSTSPPSRNLSLSLSRHHESMLPSTDPSPSVTLLSLSSLSDCNSTKSLLPSDPEEYPLTPMMGAKTPAAPPAHALNPLLDLRAESFKKEPNQSLTPTHVSAAMAMNRGHRRTPSDGAIRPRAQTLGHRRTPSDGSMPMPPPPGGLNKPANKGTQESLEIPRLPIPTSVFPVPHRRKAPAPPVPNPDAFCLISPLERPKTLEFAPRPRPTPARVRPSDAWKLSSLSRTLSSSPGSSCDSPQGLGDGEDGGGASAAAQQNLMDMDVEGQSLDQTVPLCGQQHPTALCGQQL

Target ORF information:

RefSeq Version	XM_011608379.1
Organism	Takifugu rubripes(Fugu rubripes)
Definition	Takifugu rubripes mitogen-activated protein kinase kinase kinase 10 (map3k10), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011608379.1

ORF Insert Sequence:

ATGAACCCGT ACGGCAGAGC AAGCAGCACG GCGCCCCCCC TGTCCTGCAC CAGCTGTGGG 
GGCTGCTGCT CGCCGCCTCC CCCTTGCTTA ATCCCACCAG GGCCCCCATC TACTACCTCC 
TCTTCCTCAA TGCCACCGAA TATTACCCCT CCGCCGCCGA TGTTCCCGGC CCCCGTGGTG 
CAGGTGGAGA ACGGCAGCAG CTGGAACTCT TCGTCCGGTT CCCCGGACTC CCACCCAGGT 
CGCCGCTGTG GGGTCACCAA CCCCAACCCT TACTGGACAG CAGTGTTTGA CTATGAGGCC 
ACAGCGGACG AGGAGTTAAC CCTGCGGCGC GGGGACCTCC TGGAGGTCCT CTCCAAAGAC 
TCAAAGGTGT CCGGGGATGA GGGTTGGTGG ACAGGCAAGA TCCAGGACAA GGTGGGCATC 
TTCCCCAGCA ACTATGTGAC CAAGGGGGAC GCGGCCAACT ACAAGCAGCT CGCTGCTGGA 
GGTCTGGTGG CGGGCGGGCT AGGCGAGTGT CCTCTGGAGA TAGACTTCCT AGAACTGCAC 
CTGGAAGAGG TGATCGGAGC GGGCGGGTTT GGGAAAGTGT ACAAAGGAAT GTGGAGGAAC 
GAAGAGGTAG CGGTGAAGGC CGCCAGGCAA GACCCCGACG AGGACATCAG CGCCACGGCG 
GAGAGCGTGC GCCAGGAGGC CAGGCTGTTC TGGATGCTCC AACATCGCAA CATAATCGCC 
CTGCGCGGGG TGTGCCTGCG CGAGCCCAAC CTGTGCTTGG TGATGGAGTA CGCCAGAGGG 
GGGGCGCTGA ACCGAGCACT CGCTGGAAAG AAGGTGCCCC CAAAGGTTCT GGTGAACTGG 
GCGGTCCAGA TCGCTGCGGG GATGGACTAC CTGCACAACC AGGCGTTCGT GCCCATCATT 
CACAGGGACC TCAAGTCCAG CAATATTCTC ATCCTGGAGC CTCTGGAGAG GCTCAGCTTG 
GGCAGTAAAA CCTTAAAGAT CACAGACTTC GGTCTGGCCA GGGAGTGGCA TCAGACCACC 
AAGATGAGCG CGGCGGGGAC GTACGCCTGG ATGGCCCCCG AGGTCATCAA ACTGTCCCTC 
TTCTCCAAAA GCAGCGACGT GTGGAGTTTT GGGGTGCTAC TGTGGGAGCT GCTGACCGGC 
GAGGTCCCCT ACCGAGAGAT CGACGCTCTG GCGGTAGCAT ACGGCGTCGC CATGAACAAA 
CTAACCCTCC CCATCCCGTC AACGTGCCCC GAGCCCTTCG CTCAGCTGCT GGAAGAGTGT 
TGGAGCCCAA ACCCCCACAG CAGACCCTCC TTCACCAGCA TCCTGAGGCA GCTGTTGGCC 
ATCGAGCAGT CGGCCATGTT TCAGATGCCT CTGGAGTCGT TCCACTCTTT ACAAGAGGAC 
TGGAGGCTGG AGATCCAGCA GATGTTTGAT GAACTCAGGG CCAAAGAGAA GGAACTAAGA 
TCATGGGAGG AGGCGCTGGC AAGGGCGGCC GAGGAGCAGC GGGAGCAGGA GGAGCAGCTG 
CGGAGGCGGG AGCAGGAGCT GGCGGAGAGG GAGATCGATA TCGTGGAACG AGAGCTGAAC 
ATCATCATCC ACCAGGTGTA CCAGGAGAAA CCCAGCGTGA AGAAAAGGAA AGGCCACTTT 
AAAAAGAGCA GACTGCTGAA GCTGGGCCGC GACAGCAACT GCATCAGTCT GCCATCTGGC 
TTCGAGCATA AGATCACCGT CCAGGCATCT CCCAAGGTGG ACAAGAGGAA GCCTCAGGGC 
AGCGAGAGCA CCACCCCACC CGCCAGTCCA GGGGTCATTC CCCGATTGAG AGCCATCAGA 
TTGACGCCGA CCGACGGCAG CAAGACGTGG GGGCGCAGCG CCGTGTGCAA GAAGGAGGAC 
CTGTCGAGCA GCAAGAAGAA AGGACGCACC TGGGGGCCCA GCTCCACGCA CCAGAAAGAA 
AGAGTTGGTG GAGAAGACAA GCTGAAGTCT CTGGGCGAGG GATGCAAAGT GTGGTCGTCC 
AGTGCTCCAA ATCTGGGCAA GTCCCCCAAA CATGCCCCCA TGACCGCCGG CTTCTCCAGC 
CTTAACGAAA TGGAGGAGTG CTGTGACCCG GAGGAGTCGC CCGGCTCCCT GCAGCCGTCG 
GAGACCAGCA GCAACGGCGC TGTGGACGAT TCGGGGTCTC TGTGGAGCAG CCCGTCGGCC 
ACTGGCGGCT CCTGCCAGGG CTCCGTGGGT CAGGGATCGG GGGCGAACTA CCAGGAGTCT 
GCGCGGCGCT GCAGCCAGAG GAAGAAAAGC GACATGTTGC TGCTGGGGTG TGCCTCTCTG 
CTGGCCTCCG TCGCCCTGGG ACAGGACCTC CTGCAGCTGG GAAAACTACA GGTCCTTCAG 
GATGAGCAGG ACCTCAGAGA CGAGCAGCGT AAGAAGAAAG ACGGCCTTTT CCAGAGGGCG 
GGGCGCTTCC GCCGCAGCAC CTCCCCGCCC AGCCGAAACC TCTCCCTCTC CCTCTCCCGA 
CACCATGAGT CCATGCTCCC CAGCACGGAC CCCTCGCCCT CTGTCACCCT CCTCTCCTTG 
TCCTCTCTGT CTGACTGCAA CTCCACCAAG TCCCTCCTTC CCTCTGACCC GGAGGAGTAC 
CCCCTGACCC CCATGATGGG GGCGAAAACC CCAGCGGCCC CGCCGGCTCA CGCCCTCAAC 
CCTCTGCTGG ACCTGCGGGC AGAGAGCTTT AAGAAGGAGC CCAACCAGTC TCTGACACCC 
ACCCACGTGT CTGCAGCCAT GGCCATGAAC AGAGGGCACA GACGCACCCC CTCAGACGGG 
GCCATACGGC CCCGGGCTCA GACTCTGGGA CATCGCAGGA CTCCGTCAGA CGGAAGCATG 
CCCATGCCCC CCCCACCTGG AGGACTGAAC AAACCTGCAA ACAAAGGGAC ACAGGAAAGC 
TTGGAAATCC CCCGCCTCCC CATCCCCACC TCCGTCTTCC CCGTCCCCCA CCGACGCAAA 
GCTCCCGCTC CGCCGGTCCC CAACCCCGAC GCCTTCTGCC TCATCAGCCC CCTGGAGAGG 
CCCAAAACGC TGGAATTCGC ACCCCGGCCT CGGCCCACGC CGGCCCGCGT GAGGCCGTCC 
GACGCCTGGA AGCTGAGCTC GCTGTCGCGC ACCCTCAGCT CGTCCCCGGG CAGCAGCTGT 
GACAGCCCCC AGGGTTTAGG GGACGGCGAG GACGGCGGCG GCGCTAGTGC GGCGGCGCAG 
CAAAACCTGA TGGACATGGA CGTGGAGGGT CAGAGCTTAG ACCAGACGGT CCCTCTGTGT 
GGACAGCAGC ACCCCACTGC GCTCTGTGGA CAACAACTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

map3k10 ( XM_011608379.2 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_011606681.1 Takifugu rubripes mitogen-activated protein kinase kinase kinase 10 (map3k10), mRNA.

Price & Availability↑

CloneID	OTo23942
Clone ID Related Accession (Same CDS sequence)	XM_011608379.1 , XM_011608379.2
Accession Version	XM_011608379.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3282bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-04
Organism	Takifugu rubripes(torafugu)
Product	mitogen-activated protein kinase kinase kinase 10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042295.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 5, 2019 this sequence version replaced XM_011608379.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACCCGT ACGGCAGAGC AAGCAGCACG GCGCCCCCCC TGTCCTGCAC CAGCTGTGGG 
GGCTGCTGCT CGCCGCCTCC CCCTTGCTTA ATCCCACCAG GGCCCCCATC TACTACCTCC 
TCTTCCTCAA TGCCACCGAA TATTACCCCT CCGCCGCCGA TGTTCCCGGC CCCCGTGGTG 
CAGGTGGAGA ACGGCAGCAG CTGGAACTCT TCGTCCGGTT CCCCGGACTC CCACCCAGGT 
CGCCGCTGTG GGGTCACCAA CCCCAACCCT TACTGGACAG CAGTGTTTGA CTATGAGGCC 
ACAGCGGACG AGGAGTTAAC CCTGCGGCGC GGGGACCTCC TGGAGGTCCT CTCCAAAGAC 
TCAAAGGTGT CCGGGGATGA GGGTTGGTGG ACAGGCAAGA TCCAGGACAA GGTGGGCATC 
TTCCCCAGCA ACTATGTGAC CAAGGGGGAC GCGGCCAACT ACAAGCAGCT CGCTGCTGGA 
GGTCTGGTGG CGGGCGGGCT AGGCGAGTGT CCTCTGGAGA TAGACTTCCT AGAACTGCAC 
CTGGAAGAGG TGATCGGAGC GGGCGGGTTT GGGAAAGTGT ACAAAGGAAT GTGGAGGAAC 
GAAGAGGTAG CGGTGAAGGC CGCCAGGCAA GACCCCGACG AGGACATCAG CGCCACGGCG 
GAGAGCGTGC GCCAGGAGGC CAGGCTGTTC TGGATGCTCC AACATCGCAA CATAATCGCC 
CTGCGCGGGG TGTGCCTGCG CGAGCCCAAC CTGTGCTTGG TGATGGAGTA CGCCAGAGGG 
GGGGCGCTGA ACCGAGCACT CGCTGGAAAG AAGGTGCCCC CAAAGGTTCT GGTGAACTGG 
GCGGTCCAGA TCGCTGCGGG GATGGACTAC CTGCACAACC AGGCGTTCGT GCCCATCATT 
CACAGGGACC TCAAGTCCAG CAATATTCTC ATCCTGGAGC CTCTGGAGAG GCTCAGCTTG 
GGCAGTAAAA CCTTAAAGAT CACAGACTTC GGTCTGGCCA GGGAGTGGCA TCAGACCACC 
AAGATGAGCG CGGCGGGGAC GTACGCCTGG ATGGCCCCCG AGGTCATCAA ACTGTCCCTC 
TTCTCCAAAA GCAGCGACGT GTGGAGTTTT GGGGTGCTAC TGTGGGAGCT GCTGACCGGC 
GAGGTCCCCT ACCGAGAGAT CGACGCTCTG GCGGTAGCAT ACGGCGTCGC CATGAACAAA 
CTAACCCTCC CCATCCCGTC AACGTGCCCC GAGCCCTTCG CTCAGCTGCT GGAAGAGTGT 
TGGAGCCCAA ACCCCCACAG CAGACCCTCC TTCACCAGCA TCCTGAGGCA GCTGTTGGCC 
ATCGAGCAGT CGGCCATGTT TCAGATGCCT CTGGAGTCGT TCCACTCTTT ACAAGAGGAC 
TGGAGGCTGG AGATCCAGCA GATGTTTGAT GAACTCAGGG CCAAAGAGAA GGAACTAAGA 
TCATGGGAGG AGGCGCTGGC AAGGGCGGCC GAGGAGCAGC GGGAGCAGGA GGAGCAGCTG 
CGGAGGCGGG AGCAGGAGCT GGCGGAGAGG GAGATCGATA TCGTGGAACG AGAGCTGAAC 
ATCATCATCC ACCAGGTGTA CCAGGAGAAA CCCAGCGTGA AGAAAAGGAA AGGCCACTTT 
AAAAAGAGCA GACTGCTGAA GCTGGGCCGC GACAGCAACT GCATCAGTCT GCCATCTGGC 
TTCGAGCATA AGATCACCGT CCAGGCATCT CCCAAGGTGG ACAAGAGGAA GCCTCAGGGC 
AGCGAGAGCA CCACCCCACC CGCCAGTCCA GGGGTCATTC CCCGATTGAG AGCCATCAGA 
TTGACGCCGA CCGACGGCAG CAAGACGTGG GGGCGCAGCG CCGTGTGCAA GAAGGAGGAC 
CTGTCGAGCA GCAAGAAGAA AGGACGCACC TGGGGGCCCA GCTCCACGCA CCAGAAAGAA 
AGAGTTGGTG GAGAAGACAA GCTGAAGTCT CTGGGCGAGG GATGCAAAGT GTGGTCGTCC 
AGTGCTCCAA ATCTGGGCAA GTCCCCCAAA CATGCCCCCA TGACCGCCGG CTTCTCCAGC 
CTTAACGAAA TGGAGGAGTG CTGTGACCCG GAGGAGTCGC CCGGCTCCCT GCAGCCGTCG 
GAGACCAGCA GCAACGGCGC TGTGGACGAT TCGGGGTCTC TGTGGAGCAG CCCGTCGGCC 
ACTGGCGGCT CCTGCCAGGG CTCCGTGGGT CAGGGATCGG GGGCGAACTA CCAGGAGTCT 
GCGCGGCGCT GCAGCCAGAG GAAGAAAAGC GACATGTTGC TGCTGGGGTG TGCCTCTCTG 
CTGGCCTCCG TCGCCCTGGG ACAGGACCTC CTGCAGCTGG GAAAACTACA GGTCCTTCAG 
GATGAGCAGG ACCTCAGAGA CGAGCAGCGT AAGAAGAAAG ACGGCCTTTT CCAGAGGGCG 
GGGCGCTTCC GCCGCAGCAC CTCCCCGCCC AGCCGAAACC TCTCCCTCTC CCTCTCCCGA 
CACCATGAGT CCATGCTCCC CAGCACGGAC CCCTCGCCCT CTGTCACCCT CCTCTCCTTG 
TCCTCTCTGT CTGACTGCAA CTCCACCAAG TCCCTCCTTC CCTCTGACCC GGAGGAGTAC 
CCCCTGACCC CCATGATGGG GGCGAAAACC CCAGCGGCCC CGCCGGCTCA CGCCCTCAAC 
CCTCTGCTGG ACCTGCGGGC AGAGAGCTTT AAGAAGGAGC CCAACCAGTC TCTGACACCC 
ACCCACGTGT CTGCAGCCAT GGCCATGAAC AGAGGGCACA GACGCACCCC CTCAGACGGG 
GCCATACGGC CCCGGGCTCA GACTCTGGGA CATCGCAGGA CTCCGTCAGA CGGAAGCATG 
CCCATGCCCC CCCCACCTGG AGGACTGAAC AAACCTGCAA ACAAAGGGAC ACAGGAAAGC 
TTGGAAATCC CCCGCCTCCC CATCCCCACC TCCGTCTTCC CCGTCCCCCA CCGACGCAAA 
GCTCCCGCTC CGCCGGTCCC CAACCCCGAC GCCTTCTGCC TCATCAGCCC CCTGGAGAGG 
CCCAAAACGC TGGAATTCGC ACCCCGGCCT CGGCCCACGC CGGCCCGCGT GAGGCCGTCC 
GACGCCTGGA AGCTGAGCTC GCTGTCGCGC ACCCTCAGCT CGTCCCCGGG CAGCAGCTGT 
GACAGCCCCC AGGGTTTAGG GGACGGCGAG GACGGCGGCG GCGCTAGTGC GGCGGCGCAG 
CAAAACCTGA TGGACATGGA CGTGGAGGGT CAGAGCTTAG ACCAGACGGT CCCTCTGTGT 
GGACAGCAGC ACCCCACTGC GCTCTGTGGA CAACAACTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011606681.1
CDS	1837..5118
Translation	MNPYGRASSTAPPLSCTSCGGCCSPPPPCLIPPGPPSTTSSSSMPPNITPPPPMFPAPVVQVENGSSWNSSSGSPDSHPGRRCGVTNPNPYWTAVFDYEATADEELTLRRGDLLEVLSKDSKVSGDEGWWTGKIQDKVGIFPSNYVTKGDAANYKQLAAGGLVAGGLGECPLEIDFLELHLEEVIGAGGFGKVYKGMWRNEEVAVKAARQDPDEDISATAESVRQEARLFWMLQHRNIIALRGVCLREPNLCLVMEYARGGALNRALAGKKVPPKVLVNWAVQIAAGMDYLHNQAFVPIIHRDLKSSNILILEPLERLSLGSKTLKITDFGLAREWHQTTKMSAAGTYAWMAPEVIKLSLFSKSSDVWSFGVLLWELLTGEVPYREIDALAVAYGVAMNKLTLPIPSTCPEPFAQLLEECWSPNPHSRPSFTSILRQLLAIEQSAMFQMPLESFHSLQEDWRLEIQQMFDELRAKEKELRSWEEALARAAEEQREQEEQLRRREQELAEREIDIVERELNIIIHQVYQEKPSVKKRKGHFKKSRLLKLGRDSNCISLPSGFEHKITVQASPKVDKRKPQGSESTTPPASPGVIPRLRAIRLTPTDGSKTWGRSAVCKKEDLSSSKKKGRTWGPSSTHQKERVGGEDKLKSLGEGCKVWSSSAPNLGKSPKHAPMTAGFSSLNEMEECCDPEESPGSLQPSETSSNGAVDDSGSLWSSPSATGGSCQGSVGQGSGANYQESARRCSQRKKSDMLLLGCASLLASVALGQDLLQLGKLQVLQDEQDLRDEQRKKKDGLFQRAGRFRRSTSPPSRNLSLSLSRHHESMLPSTDPSPSVTLLSLSSLSDCNSTKSLLPSDPEEYPLTPMMGAKTPAAPPAHALNPLLDLRAESFKKEPNQSLTPTHVSAAMAMNRGHRRTPSDGAIRPRAQTLGHRRTPSDGSMPMPPPPGGLNKPANKGTQESLEIPRLPIPTSVFPVPHRRKAPAPPVPNPDAFCLISPLERPKTLEFAPRPRPTPARVRPSDAWKLSSLSRTLSSSPGSSCDSPQGLGDGEDGGGASAAAQQNLMDMDVEGQSLDQTVPLCGQQHPTALCGQQL

Target ORF information:

RefSeq Version	XM_011608379.2
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes mitogen-activated protein kinase kinase kinase 10 (map3k10), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011608379.2

ORF Insert Sequence:

ATGAACCCGT ACGGCAGAGC AAGCAGCACG GCGCCCCCCC TGTCCTGCAC CAGCTGTGGG 
GGCTGCTGCT CGCCGCCTCC CCCTTGCTTA ATCCCACCAG GGCCCCCATC TACTACCTCC 
TCTTCCTCAA TGCCACCGAA TATTACCCCT CCGCCGCCGA TGTTCCCGGC CCCCGTGGTG 
CAGGTGGAGA ACGGCAGCAG CTGGAACTCT TCGTCCGGTT CCCCGGACTC CCACCCAGGT 
CGCCGCTGTG GGGTCACCAA CCCCAACCCT TACTGGACAG CAGTGTTTGA CTATGAGGCC 
ACAGCGGACG AGGAGTTAAC CCTGCGGCGC GGGGACCTCC TGGAGGTCCT CTCCAAAGAC 
TCAAAGGTGT CCGGGGATGA GGGTTGGTGG ACAGGCAAGA TCCAGGACAA GGTGGGCATC 
TTCCCCAGCA ACTATGTGAC CAAGGGGGAC GCGGCCAACT ACAAGCAGCT CGCTGCTGGA 
GGTCTGGTGG CGGGCGGGCT AGGCGAGTGT CCTCTGGAGA TAGACTTCCT AGAACTGCAC 
CTGGAAGAGG TGATCGGAGC GGGCGGGTTT GGGAAAGTGT ACAAAGGAAT GTGGAGGAAC 
GAAGAGGTAG CGGTGAAGGC CGCCAGGCAA GACCCCGACG AGGACATCAG CGCCACGGCG 
GAGAGCGTGC GCCAGGAGGC CAGGCTGTTC TGGATGCTCC AACATCGCAA CATAATCGCC 
CTGCGCGGGG TGTGCCTGCG CGAGCCCAAC CTGTGCTTGG TGATGGAGTA CGCCAGAGGG 
GGGGCGCTGA ACCGAGCACT CGCTGGAAAG AAGGTGCCCC CAAAGGTTCT GGTGAACTGG 
GCGGTCCAGA TCGCTGCGGG GATGGACTAC CTGCACAACC AGGCGTTCGT GCCCATCATT 
CACAGGGACC TCAAGTCCAG CAATATTCTC ATCCTGGAGC CTCTGGAGAG GCTCAGCTTG 
GGCAGTAAAA CCTTAAAGAT CACAGACTTC GGTCTGGCCA GGGAGTGGCA TCAGACCACC 
AAGATGAGCG CGGCGGGGAC GTACGCCTGG ATGGCCCCCG AGGTCATCAA ACTGTCCCTC 
TTCTCCAAAA GCAGCGACGT GTGGAGTTTT GGGGTGCTAC TGTGGGAGCT GCTGACCGGC 
GAGGTCCCCT ACCGAGAGAT CGACGCTCTG GCGGTAGCAT ACGGCGTCGC CATGAACAAA 
CTAACCCTCC CCATCCCGTC AACGTGCCCC GAGCCCTTCG CTCAGCTGCT GGAAGAGTGT 
TGGAGCCCAA ACCCCCACAG CAGACCCTCC TTCACCAGCA TCCTGAGGCA GCTGTTGGCC 
ATCGAGCAGT CGGCCATGTT TCAGATGCCT CTGGAGTCGT TCCACTCTTT ACAAGAGGAC 
TGGAGGCTGG AGATCCAGCA GATGTTTGAT GAACTCAGGG CCAAAGAGAA GGAACTAAGA 
TCATGGGAGG AGGCGCTGGC AAGGGCGGCC GAGGAGCAGC GGGAGCAGGA GGAGCAGCTG 
CGGAGGCGGG AGCAGGAGCT GGCGGAGAGG GAGATCGATA TCGTGGAACG AGAGCTGAAC 
ATCATCATCC ACCAGGTGTA CCAGGAGAAA CCCAGCGTGA AGAAAAGGAA AGGCCACTTT 
AAAAAGAGCA GACTGCTGAA GCTGGGCCGC GACAGCAACT GCATCAGTCT GCCATCTGGC 
TTCGAGCATA AGATCACCGT CCAGGCATCT CCCAAGGTGG ACAAGAGGAA GCCTCAGGGC 
AGCGAGAGCA CCACCCCACC CGCCAGTCCA GGGGTCATTC CCCGATTGAG AGCCATCAGA 
TTGACGCCGA CCGACGGCAG CAAGACGTGG GGGCGCAGCG CCGTGTGCAA GAAGGAGGAC 
CTGTCGAGCA GCAAGAAGAA AGGACGCACC TGGGGGCCCA GCTCCACGCA CCAGAAAGAA 
AGAGTTGGTG GAGAAGACAA GCTGAAGTCT CTGGGCGAGG GATGCAAAGT GTGGTCGTCC 
AGTGCTCCAA ATCTGGGCAA GTCCCCCAAA CATGCCCCCA TGACCGCCGG CTTCTCCAGC 
CTTAACGAAA TGGAGGAGTG CTGTGACCCG GAGGAGTCGC CCGGCTCCCT GCAGCCGTCG 
GAGACCAGCA GCAACGGCGC TGTGGACGAT TCGGGGTCTC TGTGGAGCAG CCCGTCGGCC 
ACTGGCGGCT CCTGCCAGGG CTCCGTGGGT CAGGGATCGG GGGCGAACTA CCAGGAGTCT 
GCGCGGCGCT GCAGCCAGAG GAAGAAAAGC GACATGTTGC TGCTGGGGTG TGCCTCTCTG 
CTGGCCTCCG TCGCCCTGGG ACAGGACCTC CTGCAGCTGG GAAAACTACA GGTCCTTCAG 
GATGAGCAGG ACCTCAGAGA CGAGCAGCGT AAGAAGAAAG ACGGCCTTTT CCAGAGGGCG 
GGGCGCTTCC GCCGCAGCAC CTCCCCGCCC AGCCGAAACC TCTCCCTCTC CCTCTCCCGA 
CACCATGAGT CCATGCTCCC CAGCACGGAC CCCTCGCCCT CTGTCACCCT CCTCTCCTTG 
TCCTCTCTGT CTGACTGCAA CTCCACCAAG TCCCTCCTTC CCTCTGACCC GGAGGAGTAC 
CCCCTGACCC CCATGATGGG GGCGAAAACC CCAGCGGCCC CGCCGGCTCA CGCCCTCAAC 
CCTCTGCTGG ACCTGCGGGC AGAGAGCTTT AAGAAGGAGC CCAACCAGTC TCTGACACCC 
ACCCACGTGT CTGCAGCCAT GGCCATGAAC AGAGGGCACA GACGCACCCC CTCAGACGGG 
GCCATACGGC CCCGGGCTCA GACTCTGGGA CATCGCAGGA CTCCGTCAGA CGGAAGCATG 
CCCATGCCCC CCCCACCTGG AGGACTGAAC AAACCTGCAA ACAAAGGGAC ACAGGAAAGC 
TTGGAAATCC CCCGCCTCCC CATCCCCACC TCCGTCTTCC CCGTCCCCCA CCGACGCAAA 
GCTCCCGCTC CGCCGGTCCC CAACCCCGAC GCCTTCTGCC TCATCAGCCC CCTGGAGAGG 
CCCAAAACGC TGGAATTCGC ACCCCGGCCT CGGCCCACGC CGGCCCGCGT GAGGCCGTCC 
GACGCCTGGA AGCTGAGCTC GCTGTCGCGC ACCCTCAGCT CGTCCCCGGG CAGCAGCTGT 
GACAGCCCCC AGGGTTTAGG GGACGGCGAG GACGGCGGCG GCGCTAGTGC GGCGGCGCAG 
CAAAACCTGA TGGACATGGA CGTGGAGGGT CAGAGCTTAG ACCAGACGGT CCCTCTGTGT 
GGACAGCAGC ACCCCACTGC GCTCTGTGGA CAACAACTGT AG