LOC105218310 cDNA ORF clone, Zeugodacus cucurbitae(melon fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC105218310
Entrez Gene ID	105218310
Full Name	SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily E member 1
Gene Type	protein-coding
Organism	Zeugodacus cucurbitae(melon fly)

mRNA and Protein(s)

mRNA	Protein	Name
XM_011193825.2	XP_011192127.2	uncharacterized protein CG7065
XM_011193825.1	XP_011192127.1	SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily E member 1

Pathways from BioSystems
Gene Ontology

The following LOC105218310 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC105218310 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBa205456	XM_011193825.1 Latest version!	Bactrocera cucurbitae SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily E member 1 (LOC105218310), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OBa257756	XM_011193825.2 Latest version!	Zeugodacus cucurbitae uncharacterized protein CG7065 (LOC105218310), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC105218310 ( XM_011193825.1 ) cDNA ORF clone, Zeugodacus cucurbitae(melon fly) -> XP_011192127.1 Bactrocera cucurbitae SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily E member 1 (LOC105218310), mRNA.

Price & Availability↑

CloneID	OBa205456
Clone ID Related Accession (Same CDS sequence)	XM_011193825.1
Accession Version	XM_011193825.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2088bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-01-28
Organism	Zeugodacus cucurbitae(melon fly)
Product	SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily E member 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011863852.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bactrocera cucurbitae Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCTAC CAAGCAATTA CAAACAAATT GCGGTTGGTG CGCCAACAGG ACCGCCAACT 
CCAGCACCTA GCAGCGGCTC TGGTAGTAGC CGCTCGCGTT CTTCCGGAGC TGGTGGAGGT 
TCAGACCGAA ATAAAGATAC CACACCAATT TTCACTCATA GTAACTATGG TAATCCCGCT 
TTTACTCCTC AGAAGGCAGG CAAATCATCA AGCTCAAAAA ATCAGTCCGA GTCACGGACA 
CCTAAACCTC CAAAGCCTCC AGAGAAGCCG GTACTGCCAT ACATGAGATA TTCCAAACGA 
ATTTGGGATA GTGTTAAGGC GCAGTATCCA GACCTAAAAT TATGGGAGTT GGGAAAGAAG 
ATCGGTGCAA TGTGGAAGCA GCTAACAGAA TCCGAAAAGC AGGAATTCAT TGATGAGTAT 
GAGGCCGACA AAGCTGAGTA TGAAAAAGCA TTGAAAACGT ACCACAATTC ACCGGCATAC 
CTAACATTTC TCGCTGCTAA GAGCAAAGTA AAAACCGATT CCGATGTCCA TGAGACACCT 
TCACGATCCA GTGGTAAAAG TCAGCAAGAA AGGCGGATTG ATATACAACC TGCAGAAGAC 
GAAGACGACC CGGATGAAGG ATATTCATTC AAACATGTAG CATATGCGCG CTATTTGAGA 
AATCACCGGC TCATCAATGA GATATTTTCG GACGCTGTAG TACCGGATGT GCGTTCTGTA 
GTAACTACCC AACGTATGCA GGTATTGAAA CGTCAAGTAA GCTCACTAAC TATGCATCAG 
ACTAAGCTTG AAGCTGAACT ACAACAAATG GAAGAAAAGT TCGAGGCCAA AAAACAACGT 
ATGGTTGAAT CTAGTGAGGC TTTCCAAGAA GAATTAAAGC GACACTGCAA ACCTGCGGTA 
GATGATGATA CATTCCAGAA AATGGTTGTT AAAATATATG AAGAAATTAA GCGAGAACGT 
CAACGCGTAG ATGAGCACCC CCAAGCTGCT ATTTCTACTG CGACTGTACC ACAAGTAGTG 
AGTACTCCCA ATGAAGAGAA AATCGTTTCT GCAACCGTGC AGCCTAACAC TGGTGTTATG 
CCAGCTTCAG GGAAAAAAGA TTCGAATATT GCAATTCCAA GCACACAACC ACTACAAGCT 
CCAAGAGATG GGAATGAATG CGGCAGTAAA ACAGAACCGG AGCCAATGGA CATTGAACCA 
ACCCCAAAAC CATCAGCACC GTTACCCTGT AGGCCAGAGC TTCAAAAATC TGCTGGTAAA 
ACAGGAGTTG AATCAATAAA AGATGGTAAC AACAAATCAC CAACGTCATC GAATATAAAA 
TCTGGACAAC CTGTTAAAGT ACCAACTCCA ACACCAACTC CACCTCCACA ATCGGGTCCC 
ATAGAAAACA CTACTACTAC AAATCATCCC CTACCATCTG CAACTTCAAC AAATCCACCA 
TCTCAGATTA GTGTGCCACC AGCTCAAACA CCACCACCAC AAGCTGTAAA CCAACCGAAT 
CAGCAACCAC CGCAACCACA AACTACACAG CAACCGCCGC CAACCCAACC AGTTCCATCA 
ACGATGCCAA TGCATCCACA TCATCCTCCT CCTCCAACAC ATACACACTT ACCACCTCAT 
TTACCGCCAC ATCAATCCAT GTCAGCTATG CCTCCTCATG GCGGTTATGG CAATTACGGC 
GCCCCAACTG GACCTACTCG ATCCCCGTAT TATCAACCGC AATACGGAGC GCATCCTGCT 
CAGCCCTACG GTCAATATGC TCCTTATCCA CACTACCAAC AACCTTACGG TCCACCCCCA 
GGCTCACATT ATATGAATCC TCGACCACCA CCACAACACA ACGGTTCGCC TGTACATTAT 
CCCGAGCATG GTGGACCTGT GTCAGCACAA CCTCAGCCAC ATGATGTTTA TGGTCAACAA 
CCACCCCATA ACCCTGTTTT ACCACAACAA CAACCAGTAA CTGGTACAGT CCCACCTTTA 
GCTGCTGCAG GATCAGGCGG CACCGGTGGC CCAACGCCTG CTTCTGGTAC CACGGTATCT 
GAGATTGGCA AAAGTGAGAT TGAAAAGAAA GATTCTGGCG CTGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011192127.1
CDS	104..2191
Translation	MSLPSNYKQIAVGAPTGPPTPAPSSGSGSSRSRSSGAGGGSDRNKDTTPIFTHSNYGNPAFTPQKAGKSSSSKNQSESRTPKPPKPPEKPVLPYMRYSKRIWDSVKAQYPDLKLWELGKKIGAMWKQLTESEKQEFIDEYEADKAEYEKALKTYHNSPAYLTFLAAKSKVKTDSDVHETPSRSSGKSQQERRIDIQPAEDEDDPDEGYSFKHVAYARYLRNHRLINEIFSDAVVPDVRSVVTTQRMQVLKRQVSSLTMHQTKLEAELQQMEEKFEAKKQRMVESSEAFQEELKRHCKPAVDDDTFQKMVVKIYEEIKRERQRVDEHPQAAISTATVPQVVSTPNEEKIVSATVQPNTGVMPASGKKDSNIAIPSTQPLQAPRDGNECGSKTEPEPMDIEPTPKPSAPLPCRPELQKSAGKTGVESIKDGNNKSPTSSNIKSGQPVKVPTPTPTPPPQSGPIENTTTTNHPLPSATSTNPPSQISVPPAQTPPPQAVNQPNQQPPQPQTTQQPPPTQPVPSTMPMHPHHPPPPTHTHLPPHLPPHQSMSAMPPHGGYGNYGAPTGPTRSPYYQPQYGAHPAQPYGQYAPYPHYQQPYGPPPGSHYMNPRPPPQHNGSPVHYPEHGGPVSAQPQPHDVYGQQPPHNPVLPQQQPVTGTVPPLAAAGSGGTGGPTPASGTTVSEIGKSEIEKKDSGAE

Target ORF information:

RefSeq Version	XM_011193825.1
Organism	Zeugodacus cucurbitae(melon fly)
Definition	Bactrocera cucurbitae SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily E member 1 (LOC105218310), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011193825.1

ORF Insert Sequence:

ATGTCTCTAC CAAGCAATTA CAAACAAATT GCGGTTGGTG CGCCAACAGG ACCGCCAACT 
CCAGCACCTA GCAGCGGCTC TGGTAGTAGC CGCTCGCGTT CTTCCGGAGC TGGTGGAGGT 
TCAGACCGAA ATAAAGATAC CACACCAATT TTCACTCATA GTAACTATGG TAATCCCGCT 
TTTACTCCTC AGAAGGCAGG CAAATCATCA AGCTCAAAAA ATCAGTCCGA GTCACGGACA 
CCTAAACCTC CAAAGCCTCC AGAGAAGCCG GTACTGCCAT ACATGAGATA TTCCAAACGA 
ATTTGGGATA GTGTTAAGGC GCAGTATCCA GACCTAAAAT TATGGGAGTT GGGAAAGAAG 
ATCGGTGCAA TGTGGAAGCA GCTAACAGAA TCCGAAAAGC AGGAATTCAT TGATGAGTAT 
GAGGCCGACA AAGCTGAGTA TGAAAAAGCA TTGAAAACGT ACCACAATTC ACCGGCATAC 
CTAACATTTC TCGCTGCTAA GAGCAAAGTA AAAACCGATT CCGATGTCCA TGAGACACCT 
TCACGATCCA GTGGTAAAAG TCAGCAAGAA AGGCGGATTG ATATACAACC TGCAGAAGAC 
GAAGACGACC CGGATGAAGG ATATTCATTC AAACATGTAG CATATGCGCG CTATTTGAGA 
AATCACCGGC TCATCAATGA GATATTTTCG GACGCTGTAG TACCGGATGT GCGTTCTGTA 
GTAACTACCC AACGTATGCA GGTATTGAAA CGTCAAGTAA GCTCACTAAC TATGCATCAG 
ACTAAGCTTG AAGCTGAACT ACAACAAATG GAAGAAAAGT TCGAGGCCAA AAAACAACGT 
ATGGTTGAAT CTAGTGAGGC TTTCCAAGAA GAATTAAAGC GACACTGCAA ACCTGCGGTA 
GATGATGATA CATTCCAGAA AATGGTTGTT AAAATATATG AAGAAATTAA GCGAGAACGT 
CAACGCGTAG ATGAGCACCC CCAAGCTGCT ATTTCTACTG CGACTGTACC ACAAGTAGTG 
AGTACTCCCA ATGAAGAGAA AATCGTTTCT GCAACCGTGC AGCCTAACAC TGGTGTTATG 
CCAGCTTCAG GGAAAAAAGA TTCGAATATT GCAATTCCAA GCACACAACC ACTACAAGCT 
CCAAGAGATG GGAATGAATG CGGCAGTAAA ACAGAACCGG AGCCAATGGA CATTGAACCA 
ACCCCAAAAC CATCAGCACC GTTACCCTGT AGGCCAGAGC TTCAAAAATC TGCTGGTAAA 
ACAGGAGTTG AATCAATAAA AGATGGTAAC AACAAATCAC CAACGTCATC GAATATAAAA 
TCTGGACAAC CTGTTAAAGT ACCAACTCCA ACACCAACTC CACCTCCACA ATCGGGTCCC 
ATAGAAAACA CTACTACTAC AAATCATCCC CTACCATCTG CAACTTCAAC AAATCCACCA 
TCTCAGATTA GTGTGCCACC AGCTCAAACA CCACCACCAC AAGCTGTAAA CCAACCGAAT 
CAGCAACCAC CGCAACCACA AACTACACAG CAACCGCCGC CAACCCAACC AGTTCCATCA 
ACGATGCCAA TGCATCCACA TCATCCTCCT CCTCCAACAC ATACACACTT ACCACCTCAT 
TTACCGCCAC ATCAATCCAT GTCAGCTATG CCTCCTCATG GCGGTTATGG CAATTACGGC 
GCCCCAACTG GACCTACTCG ATCCCCGTAT TATCAACCGC AATACGGAGC GCATCCTGCT 
CAGCCCTACG GTCAATATGC TCCTTATCCA CACTACCAAC AACCTTACGG TCCACCCCCA 
GGCTCACATT ATATGAATCC TCGACCACCA CCACAACACA ACGGTTCGCC TGTACATTAT 
CCCGAGCATG GTGGACCTGT GTCAGCACAA CCTCAGCCAC ATGATGTTTA TGGTCAACAA 
CCACCCCATA ACCCTGTTTT ACCACAACAA CAACCAGTAA CTGGTACAGT CCCACCTTTA 
GCTGCTGCAG GATCAGGCGG CACCGGTGGC CCAACGCCTG CTTCTGGTAC CACGGTATCT 
GAGATTGGCA AAAGTGAGAT TGAAAAGAAA GATTCTGGCG CTGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC105218310 ( XM_011193825.2 ) cDNA ORF clone, Zeugodacus cucurbitae(melon fly) -> XP_011192127.2 Zeugodacus cucurbitae uncharacterized protein CG7065 (LOC105218310), mRNA.

Price & Availability↑

CloneID	OBa257756
Clone ID Related Accession (Same CDS sequence)	XM_011193825.2
Accession Version	XM_011193825.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5769bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-27
Organism	Zeugodacus cucurbitae(melon fly)
Product	uncharacterized protein CG7065
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011863852.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 28, 2019 this sequence version replaced XM_011193825.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Zeugodacus cucurbitae Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 2% of CDS bases ##RefSeq-Attributes-END##

ATGAATATCA TTGCTGGAGA ACCCGTTCCG CCTGGTTTTG AAGATGAAAT AAATCGTGAC 
CCTCAAATCC AAAAGGCGAT TGATCAATAC AAAGGTAATG CTCTTGTTGG ACTTGAGTAT 
ACACTTGAAT TGCATGAGGA TGACCGTCGT GAGCCGGGTT ACTTTTGTGT ACTTTGTGAT 
AAGCGTGGAG ATCCACGCAC CATAATGCAG CACTGGACAT CTTATAATCA TCGTCTAAAG 
TATCTAGAAA AACATTTCCC AACGGCGGTT CGTGAATTCA ATCCCGTAAA GTACAAGCCG 
AATTCGCATC AGATTCTTTT GAGTGTTGTG CAAAAATTAT CTAAAGCTAT TGAGGATCAC 
CATGGTCGTT TAAAATTATT GGTTGCCAAT AGCGATGATT TTCGTCGTAT GAAAGCAAAA 
TTTGTTGGAG AAATTAATAA TAAGTTCCAT TTCGACGAAC GGAATGGCCC AAACTTTCTT 
GATGTGATTG ACAGGTCTCT TTGGGAACCC ATTTCGACTG CAACCACAAA TAAGATGGAA 
CAAACGTTAC AAATAACTAT TAAAGCTCCA AAACGTATAA ATGAAGATTT TTCTATGAAA 
TCAAATCGCG ATGACTTGGA CGAAATTTCA AGTGACTCCG ACGGACAGTC AAGTTCAAAG 
AAAGTAAAAA AGCGAGAACA ATCTCCCGCT TATCGAGTGT TGCCGCGGGT TCGACGTTCG 
CCGCCTTTCG AAGAGGAACG AAGTGAAGAG CGTAAGGAAG CCGAAAAGAA ACTTCCAACA 
CCAAAAGAAT TATCCCTGCA AGCTTCGCAC ATTGCTCAGG AAAAATATAA GTGGGAAAAA 
TATAGATGCA TGCTTGATAT ACAACTTAAA CAAATGGAAG AAGTGCAACA AGATTATGAT 
AAGAACCCGG AAAAACATCC TCTGTATCCT GATGAATGGA AGAAGTTCTG GAATCGACGT 
TATAAGGAGT TACAGATAGA AAAGAAGACA GATCCCAATA AATACGATTA CAAACCTGAA 
TGGATTACTT TCTGGACTAA ACGTATGAAG GATTTATTCA ATGCTGAAGT AGAAAAAAAG 
AAGAAAGAAA TTAAAGTGAA AATTGGTTTG CCAGAAAATG CCGAGGAGAT GGTCGACCAA 
CTGAAGGAAA AATACAAAGT ACATATTGCG CGAAATGCAG TTGGTGTAAC GTCTGGTGGC 
CACGTTTTAG GACCAAAGGT TTCCGATCGT GGTATTGACA TTGCCAAAAA ACGGTCCAAA 
GCTTCGGGCG ATATTATAAA TATAAGTGAC GATGAATCTC CGGTTCGCGG TCGAGGTCGC 
CGTACACGAA CACGAACACG TTCACGTTCC CATTCTCGTT CACGACGACC CCGATCCAAG 
TCGCCACGTA ACTCACGCAG TCGCGAATAT AAGCGGTCGC GTGATCGATC CTCTTCATTG 
TCAAAAAGTC CACTTTCGGA TGATTACAAT GCGTATCCTA TGCATTCCCA GCGTCATCGC 
CATCCATCAC GAGGTTTTGC ACCATCGGTT GAAAGTGATT ATTTCAGTTC ATCGTATCCA 
CCACGCGGGA GATATGCAGC AGTACCGTCT TCAGCGCATT ATGGTGGATC AGCACATTAT 
CGCATTCTCG AACCTCACCA GTATCCAAAA TATGAACGTT CACCATCTCC GAATTCTAAA 
TCTGCAACGA CCAGAGAAGA AGCTGAACCT GAAAACGATG GGCCGCTGAC GGTTGTTGCA 
GTATTGCGTT TGCTTACAGC TCTTGAGGAC CATCTGGGCA GTCTTGGACC AAAAGTAGTA 
GATTTATTGG GCAAAGCTTT GGCATTGGAG AAGGTGAAGG CCAATTCGGC AGACGATTTG 
TTGTTGAATG AGGATCACGT CGTATTTTTT GAAACAATCA AAGAGAAATT GAAAGGTATA 
ATAATTGCGG ACGTGCTAGA CGACCAACAA AAAATCCGAG CTGTTAAGAA AGCTATAAAA 
AATATTGCGA CCGTAATTCA CCAAGTTTCT TCAAAAGGTA ACTCATCAAA TATTAATAAC 
CCTGAGGTTG CAAACACTAA CGATAACGGC GAATCAGCCA ATAAAGTGAA CCCATTAAAG 
AAATCCGACA GTGTTAGTAT TAAAGAATTA CCATTTGACC GCCAACTTAT TTCCACCAAA 
TTGGCTGCCG CTTTAGTTTT TCAAGGACGC GAGGATGTAT CCACCGAGGA TATGAATAAG 
TTGATTTTCT TATTCCTTTT ATTCGTGAAA CTATCGAAAG AAAAACGAGA AATAGATGGT 
AAAGCTTTAC AACTCGGAGG TTTGTTAGCC CAATTAGGTA TATATGTACC AGCGTCGTCC 
ACACCGACAT CTGCCGCATT GGCTGCCTTA CCAGGTATTG TGGAAGAGTT ATTGGAAAAT 
GAAATGAGTG TCGGTAGTGT TCGTGGCCAC ATTAATGAAA ACGATGCTTC AAATGAGACA 
GTCGATAAAG AAACAAAGAA TGATGGTGCT GGCAACATGT TGGAATCTTT AACCGACTCT 
GATTTACAAA CGCTATTGCA AAACTTTAAG CACTTATCGA GTGAGGAGCA ACATCATTTG 
ATAGCACATC TAAAAAAGTT GGAAACAGCT GATCCAATTC GTGTTGAGAA ACTGAGAAAA 
TTTGTTAATC TGGCTGACCT AACAGGCGCT GCTTATTCAT CACCATCAGC TGGTACAAGT 
AAGTCCAATC ACCGAGAAAG GTCGCGTACT CGTGATAGTC GTTTTGATGA TTCGCCGGTT 
CCAAGTAGTA GCCGAGGACG TAGTCACCAC GACGATGATG ATTATGATGA TCGCCGTCGT 
GGTGCCAGTG CTTCTAATCC AAATAGATTT AGTCTAGATG ATGAAGACGA GGATGATGAT 
TATAATGTAG ATGATGTGTT TAAGGCAGCG TTCCGCAAAG TAAGTGACAA TTCACGTCAA 
TCTAAAGCCG ACTCCATGTT GTCCCACGAG CCCAGTACGT CTCGCTCCCT AATGAAGCCG 
GCGCCTGTAC AAGCTCCAGC ACCTATTGTT CCGGTGTCAT CTTCACCGAA TGCGTTGACC 
TTTAATCGTG CTGGCCTTAA TATATCTCTG AGCGATACGC AAAATTTGAT TGCCAATTTG 
ATGGGCTCAT TGCAACAGAG TAATTCAAAT CAGAAAAACA ACATTAAAAC GACACAAACA 
AATGATGGCA AAGGACAACA GATACCGACC AGATCAAATG TGCCAATTCC GCCCAAAGTT 
CCGAAAAATC CAACAATGCA ACAGCAGCAA AACCCATCAG GGCCGTCAAC TACTATGCCT 
TACTATCAAC AAGGTCAGCA GCCACAGCCG CAAGCATATC CAGGCGGTGG TGGCTACAAT 
CAAATGGGTG CGATGAATAA TCAGTCATAT TATAATACTA TGGGCTATAA TAACAGTGGC 
GGACCTGGTT ATCCGGGCTA CCAACAATGG GGTATGGGAG ATGGTCAGCA ATACGGCATG 
CAAAATTTCA TGGGACAGCA ACCACAAGGT CCTCCGCAAA ATATGGGCTA CAATAATGTG 
TATGGCCAAG GCCCTCATAT TGAAACGGAG ACACGTGCCA TTGAAGAGAG GCCAAAACAC 
ACGGAGGAAA TCTCAGTTGA TGACCACAAC AGAAATATAG AGTGTAAGGA CGGTTCGGTA 
GTTACAAATT ATAAACACAT AATGTCTCTA CCAAGCAATT ACAAACAAAT TGCGGTTGGT 
GCGCCAACAG GACCGCCAAC TCCAGCACCT AGCAGCGGCT CTGGTAGTAG CCGCTCGCGT 
TCTTCCGGAG CTGGTGGAGG TTCAGACCGA AATAAAGATA CCACACCAAT TTTCACTCAT 
AGTAACTATG GTAATCCCGC TTTTACTCCT CAGAAGGCAG GCAAATCATC AAGCTCAAAA 
AATCAGTCCG AGTCACGGAC ACCTAAACCT CCAAAGCCTC CAGAGAAGCC GGTACTGCCA 
TACATGAGAT ATTCCAAACG AATTTGGGAT AGTGTTAAGG CGCAGTATCC AGACCTAAAA 
TTATGGGAGT TGGGAAAGAA GATCGGTGCA ATGTGGAAGC AGCTAACAGA ATCCGAAAAG 
CAGGAATTCA TTGATGAGTA TGAGGCCGAC AAAGCTGAGT ATGAAAAAGC ATTGAAAACG 
TACCACAATT CACCGGCATA CCTAACATTT CTCGCTGCTA AGAGCAAAGT AAAAACCGAT 
TCCGATGTCC ATGAGACACC TTCACGATCC AGTGGTAAAA GTCAGCAAGA AAGGCGGATT 
GATATACAAC CTGCAGAAGA CGAAGACGAC CCGGATGAAG GATATTCATT CAAACATGTA 
GCATATGCGC GCTATTTGAG AAATCACCGG CTCATCAATG AGATATTTTC GGACGCTGTA 
GTACCGGATG TGCGTTCTGT AGTAACTACC CAACGTATGC AGGTATTGAA ACGTCAAGTA 
AGCTCACTAA CTATGCATCA GACTAAGCTT GAAGCTGAAC TACAACAAAT GGAAGAAAAG 
TTCGAGGCCA AAAAACAACG TATGGTTGAA TCTAGTGAGG CTTTCCAAGA AGAATTAAAG 
CGACACTGCA AACCTGCGGT AGATGATGAT ACATTCCAGA AAATGGTTGT TAAAATATAT 
GAAGAAATTA AGCGAGAACG TCAACGCGTA GATGAGCACC CCCAAGCTGC TATTTCTACT 
GCGACTGTAC CACAAGTAGT GAGTACTCCC AATGAAGAGA AAATCGTTTC TGCAACCGTG 
CAGCCTAACA CTGGTGTTAT GCCAGCTTCA GGGAAAAAAG ATTCGAATAT TGCAATTCCA 
AGCACACAAC CACTACAAGC TCCAAGAGAT GGGAATGAAT GCGGCAGTAA AACAGAACCG 
GAGCCAATGG ACATTGAACC AACCCCAAAA CCATCAGCAC CGTTACCCTG TAGGCCAGAG 
CTTCAAAAAT CTGCTGGTAA AACAGGAGTT GAATCAATAA AAGATGGTAA CAACAAATCA 
CCAACGTCAT CGAATATAAA ATCTGGACAA CCTGTTAAAG TACCAACTCC AACACCAACT 
CCACCTCCAC AATCGGGTCC CATAGAAAAC ACTACTACTA CAAATCATCC CCTACCATCT 
GCAACTTCAA CAAATCCACC ATCTCAGATT AGTGTGCCAC CAGCTCAAAC ACCACCACCA 
CAAGCTGTAA ACCAACCGAA TCAGCAACCA CCGCAACCAC AAACTACACA GCAACCGCCG 
CCAACCCAAC CAGTTCCATC AACGATGCCA ATGCATCCAC ATCATCCTCC TCCTCCAACA 
CATACACACT TACCACCTCA TTTACCGCCA CATCAATCCA TGTCAGCTAT GCCTCCTCAT 
GGCGGTTATG GCAATTACGG CGCCCCAACT GGACCTACTC GATCCCCGTA TTATCAACCG 
CAATACGGAG CGCATCCTGC TCAGCCCTAC GGTCAATATG CTCCTTATCC ACACTACCAA 
CAACCTTACG GTCCACCCCC AGGCTCACAT TATATGAATC CTCGACCACC ACCACAACAC 
AACGGTTCGC CTGTACATTA TCCCGAGCAT GGTGGACCTG TGTCAGCACA ACCTCAGCCA 
CATGATGTTT ATGGTCAACA ACCACCCCAT AACCCTGTTT TACCACAACA ACAACCAGTA 
ACTGGTACAG TCCCACCTTT AGCTGCTGCA GGATCAGGCG GCACCGGTGG CCCAACGCCT 
GCTTCTGGTA CCACGGTATC TGAGATTGGC AAAAGTGAGA TTGAAAAGAA AGATTCTGGC 
GCTGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011192127.2
CDS	464..6232
Translation	MNIIAGEPVPPGFEDEINRDPQIQKAIDQYKGNALVGLEYTLELHEDDRREPGYFCVLCDKRGDPRTIMQHWTSYNHRLKYLEKHFPTAVREFNPVKYKPNSHQILLSVVQKLSKAIEDHHGRLKLLVANSDDFRRMKAKFVGEINNKFHFDERNGPNFLDVIDRSLWEPISTATTNKMEQTLQITIKAPKRINEDFSMKSNRDDLDEISSDSDGQSSSKKVKKREQSPAYRVLPRVRRSPPFEEERSEERKEAEKKLPTPKELSLQASHIAQEKYKWEKYRCMLDIQLKQMEEVQQDYDKNPEKHPLYPDEWKKFWNRRYKELQIEKKTDPNKYDYKPEWITFWTKRMKDLFNAEVEKKKKEIKVKIGLPENAEEMVDQLKEKYKVHIARNAVGVTSGGHVLGPKVSDRGIDIAKKRSKASGDIINISDDESPVRGRGRRTRTRTRSRSHSRSRRPRSKSPRNSRSREYKRSRDRSSSLSKSPLSDDYNAYPMHSQRHRHPSRGFAPSVESDYFSSSYPPRGRYAAVPSSAHYGGSAHYRILEPHQYPKYERSPSPNSKSATTREEAEPENDGPLTVVAVLRLLTALEDHLGSLGPKVVDLLGKALALEKVKANSADDLLLNEDHVVFFETIKEKLKGIIIADVLDDQQKIRAVKKAIKNIATVIHQVSSKGNSSNINNPEVANTNDNGESANKVNPLKKSDSVSIKELPFDRQLISTKLAAALVFQGREDVSTEDMNKLIFLFLLFVKLSKEKREIDGKALQLGGLLAQLGIYVPASSTPTSAALAALPGIVEELLENEMSVGSVRGHINENDASNETVDKETKNDGAGNMLESLTDSDLQTLLQNFKHLSSEEQHHLIAHLKKLETADPIRVEKLRKFVNLADLTGAAYSSPSAGTSKSNHRERSRTRDSRFDDSPVPSSSRGRSHHDDDDYDDRRRGASASNPNRFSLDDEDEDDDYNVDDVFKAAFRKVSDNSRQSKADSMLSHEPSTSRSLMKPAPVQAPAPIVPVSSSPNALTFNRAGLNISLSDTQNLIANLMGSLQQSNSNQKNNIKTTQTNDGKGQQIPTRSNVPIPPKVPKNPTMQQQQNPSGPSTTMPYYQQGQQPQPQAYPGGGGYNQMGAMNNQSYYNTMGYNNSGGPGYPGYQQWGMGDGQQYGMQNFMGQQPQGPPQNMGYNNVYGQGPHIETETRAIEERPKHTEEISVDDHNRNIECKDGSVVTNYKHIMSLPSNYKQIAVGAPTGPPTPAPSSGSGSSRSRSSGAGGGSDRNKDTTPIFTHSNYGNPAFTPQKAGKSSSSKNQSESRTPKPPKPPEKPVLPYMRYSKRIWDSVKAQYPDLKLWELGKKIGAMWKQLTESEKQEFIDEYEADKAEYEKALKTYHNSPAYLTFLAAKSKVKTDSDVHETPSRSSGKSQQERRIDIQPAEDEDDPDEGYSFKHVAYARYLRNHRLINEIFSDAVVPDVRSVVTTQRMQVLKRQVSSLTMHQTKLEAELQQMEEKFEAKKQRMVESSEAFQEELKRHCKPAVDDDTFQKMVVKIYEEIKRERQRVDEHPQAAISTATVPQVVSTPNEEKIVSATVQPNTGVMPASGKKDSNIAIPSTQPLQAPRDGNECGSKTEPEPMDIEPTPKPSAPLPCRPELQKSAGKTGVESIKDGNNKSPTSSNIKSGQPVKVPTPTPTPPPQSGPIENTTTTNHPLPSATSTNPPSQISVPPAQTPPPQAVNQPNQQPPQPQTTQQPPPTQPVPSTMPMHPHHPPPPTHTHLPPHLPPHQSMSAMPPHGGYGNYGAPTGPTRSPYYQPQYGAHPAQPYGQYAPYPHYQQPYGPPPGSHYMNPRPPPQHNGSPVHYPEHGGPVSAQPQPHDVYGQQPPHNPVLPQQQPVTGTVPPLAAAGSGGTGGPTPASGTTVSEIGKSEIEKKDSGAE

Target ORF information:

RefSeq Version	XM_011193825.2
Organism	Zeugodacus cucurbitae(melon fly)
Definition	Zeugodacus cucurbitae uncharacterized protein CG7065 (LOC105218310), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011193825.2

ORF Insert Sequence:

ATGAATATCA TTGCTGGAGA ACCCGTTCCG CCTGGTTTTG AAGATGAAAT AAATCGTGAC 
CCTCAAATCC AAAAGGCGAT TGATCAATAC AAAGGTAATG CTCTTGTTGG ACTTGAGTAT 
ACACTTGAAT TGCATGAGGA TGACCGTCGT GAGCCGGGTT ACTTTTGTGT ACTTTGTGAT 
AAGCGTGGAG ATCCACGCAC CATAATGCAG CACTGGACAT CTTATAATCA TCGTCTAAAG 
TATCTAGAAA AACATTTCCC AACGGCGGTT CGTGAATTCA ATCCCGTAAA GTACAAGCCG 
AATTCGCATC AGATTCTTTT GAGTGTTGTG CAAAAATTAT CTAAAGCTAT TGAGGATCAC 
CATGGTCGTT TAAAATTATT GGTTGCCAAT AGCGATGATT TTCGTCGTAT GAAAGCAAAA 
TTTGTTGGAG AAATTAATAA TAAGTTCCAT TTCGACGAAC GGAATGGCCC AAACTTTCTT 
GATGTGATTG ACAGGTCTCT TTGGGAACCC ATTTCGACTG CAACCACAAA TAAGATGGAA 
CAAACGTTAC AAATAACTAT TAAAGCTCCA AAACGTATAA ATGAAGATTT TTCTATGAAA 
TCAAATCGCG ATGACTTGGA CGAAATTTCA AGTGACTCCG ACGGACAGTC AAGTTCAAAG 
AAAGTAAAAA AGCGAGAACA ATCTCCCGCT TATCGAGTGT TGCCGCGGGT TCGACGTTCG 
CCGCCTTTCG AAGAGGAACG AAGTGAAGAG CGTAAGGAAG CCGAAAAGAA ACTTCCAACA 
CCAAAAGAAT TATCCCTGCA AGCTTCGCAC ATTGCTCAGG AAAAATATAA GTGGGAAAAA 
TATAGATGCA TGCTTGATAT ACAACTTAAA CAAATGGAAG AAGTGCAACA AGATTATGAT 
AAGAACCCGG AAAAACATCC TCTGTATCCT GATGAATGGA AGAAGTTCTG GAATCGACGT 
TATAAGGAGT TACAGATAGA AAAGAAGACA GATCCCAATA AATACGATTA CAAACCTGAA 
TGGATTACTT TCTGGACTAA ACGTATGAAG GATTTATTCA ATGCTGAAGT AGAAAAAAAG 
AAGAAAGAAA TTAAAGTGAA AATTGGTTTG CCAGAAAATG CCGAGGAGAT GGTCGACCAA 
CTGAAGGAAA AATACAAAGT ACATATTGCG CGAAATGCAG TTGGTGTAAC GTCTGGTGGC 
CACGTTTTAG GACCAAAGGT TTCCGATCGT GGTATTGACA TTGCCAAAAA ACGGTCCAAA 
GCTTCGGGCG ATATTATAAA TATAAGTGAC GATGAATCTC CGGTTCGCGG TCGAGGTCGC 
CGTACACGAA CACGAACACG TTCACGTTCC CATTCTCGTT CACGACGACC CCGATCCAAG 
TCGCCACGTA ACTCACGCAG TCGCGAATAT AAGCGGTCGC GTGATCGATC CTCTTCATTG 
TCAAAAAGTC CACTTTCGGA TGATTACAAT GCGTATCCTA TGCATTCCCA GCGTCATCGC 
CATCCATCAC GAGGTTTTGC ACCATCGGTT GAAAGTGATT ATTTCAGTTC ATCGTATCCA 
CCACGCGGGA GATATGCAGC AGTACCGTCT TCAGCGCATT ATGGTGGATC AGCACATTAT 
CGCATTCTCG AACCTCACCA GTATCCAAAA TATGAACGTT CACCATCTCC GAATTCTAAA 
TCTGCAACGA CCAGAGAAGA AGCTGAACCT GAAAACGATG GGCCGCTGAC GGTTGTTGCA 
GTATTGCGTT TGCTTACAGC TCTTGAGGAC CATCTGGGCA GTCTTGGACC AAAAGTAGTA 
GATTTATTGG GCAAAGCTTT GGCATTGGAG AAGGTGAAGG CCAATTCGGC AGACGATTTG 
TTGTTGAATG AGGATCACGT CGTATTTTTT GAAACAATCA AAGAGAAATT GAAAGGTATA 
ATAATTGCGG ACGTGCTAGA CGACCAACAA AAAATCCGAG CTGTTAAGAA AGCTATAAAA 
AATATTGCGA CCGTAATTCA CCAAGTTTCT TCAAAAGGTA ACTCATCAAA TATTAATAAC 
CCTGAGGTTG CAAACACTAA CGATAACGGC GAATCAGCCA ATAAAGTGAA CCCATTAAAG 
AAATCCGACA GTGTTAGTAT TAAAGAATTA CCATTTGACC GCCAACTTAT TTCCACCAAA 
TTGGCTGCCG CTTTAGTTTT TCAAGGACGC GAGGATGTAT CCACCGAGGA TATGAATAAG 
TTGATTTTCT TATTCCTTTT ATTCGTGAAA CTATCGAAAG AAAAACGAGA AATAGATGGT 
AAAGCTTTAC AACTCGGAGG TTTGTTAGCC CAATTAGGTA TATATGTACC AGCGTCGTCC 
ACACCGACAT CTGCCGCATT GGCTGCCTTA CCAGGTATTG TGGAAGAGTT ATTGGAAAAT 
GAAATGAGTG TCGGTAGTGT TCGTGGCCAC ATTAATGAAA ACGATGCTTC AAATGAGACA 
GTCGATAAAG AAACAAAGAA TGATGGTGCT GGCAACATGT TGGAATCTTT AACCGACTCT 
GATTTACAAA CGCTATTGCA AAACTTTAAG CACTTATCGA GTGAGGAGCA ACATCATTTG 
ATAGCACATC TAAAAAAGTT GGAAACAGCT GATCCAATTC GTGTTGAGAA ACTGAGAAAA 
TTTGTTAATC TGGCTGACCT AACAGGCGCT GCTTATTCAT CACCATCAGC TGGTACAAGT 
AAGTCCAATC ACCGAGAAAG GTCGCGTACT CGTGATAGTC GTTTTGATGA TTCGCCGGTT 
CCAAGTAGTA GCCGAGGACG TAGTCACCAC GACGATGATG ATTATGATGA TCGCCGTCGT 
GGTGCCAGTG CTTCTAATCC AAATAGATTT AGTCTAGATG ATGAAGACGA GGATGATGAT 
TATAATGTAG ATGATGTGTT TAAGGCAGCG TTCCGCAAAG TAAGTGACAA TTCACGTCAA 
TCTAAAGCCG ACTCCATGTT GTCCCACGAG CCCAGTACGT CTCGCTCCCT AATGAAGCCG 
GCGCCTGTAC AAGCTCCAGC ACCTATTGTT CCGGTGTCAT CTTCACCGAA TGCGTTGACC 
TTTAATCGTG CTGGCCTTAA TATATCTCTG AGCGATACGC AAAATTTGAT TGCCAATTTG 
ATGGGCTCAT TGCAACAGAG TAATTCAAAT CAGAAAAACA ACATTAAAAC GACACAAACA 
AATGATGGCA AAGGACAACA GATACCGACC AGATCAAATG TGCCAATTCC GCCCAAAGTT 
CCGAAAAATC CAACAATGCA ACAGCAGCAA AACCCATCAG GGCCGTCAAC TACTATGCCT 
TACTATCAAC AAGGTCAGCA GCCACAGCCG CAAGCATATC CAGGCGGTGG TGGCTACAAT 
CAAATGGGTG CGATGAATAA TCAGTCATAT TATAATACTA TGGGCTATAA TAACAGTGGC 
GGACCTGGTT ATCCGGGCTA CCAACAATGG GGTATGGGAG ATGGTCAGCA ATACGGCATG 
CAAAATTTCA TGGGACAGCA ACCACAAGGT CCTCCGCAAA ATATGGGCTA CAATAATGTG 
TATGGCCAAG GCCCTCATAT TGAAACGGAG ACACGTGCCA TTGAAGAGAG GCCAAAACAC 
ACGGAGGAAA TCTCAGTTGA TGACCACAAC AGAAATATAG AGTGTAAGGA CGGTTCGGTA 
GTTACAAATT ATAAACACAT AATGTCTCTA CCAAGCAATT ACAAACAAAT TGCGGTTGGT 
GCGCCAACAG GACCGCCAAC TCCAGCACCT AGCAGCGGCT CTGGTAGTAG CCGCTCGCGT 
TCTTCCGGAG CTGGTGGAGG TTCAGACCGA AATAAAGATA CCACACCAAT TTTCACTCAT 
AGTAACTATG GTAATCCCGC TTTTACTCCT CAGAAGGCAG GCAAATCATC AAGCTCAAAA 
AATCAGTCCG AGTCACGGAC ACCTAAACCT CCAAAGCCTC CAGAGAAGCC GGTACTGCCA 
TACATGAGAT ATTCCAAACG AATTTGGGAT AGTGTTAAGG CGCAGTATCC AGACCTAAAA 
TTATGGGAGT TGGGAAAGAA GATCGGTGCA ATGTGGAAGC AGCTAACAGA ATCCGAAAAG 
CAGGAATTCA TTGATGAGTA TGAGGCCGAC AAAGCTGAGT ATGAAAAAGC ATTGAAAACG 
TACCACAATT CACCGGCATA CCTAACATTT CTCGCTGCTA AGAGCAAAGT AAAAACCGAT 
TCCGATGTCC ATGAGACACC TTCACGATCC AGTGGTAAAA GTCAGCAAGA AAGGCGGATT 
GATATACAAC CTGCAGAAGA CGAAGACGAC CCGGATGAAG GATATTCATT CAAACATGTA 
GCATATGCGC GCTATTTGAG AAATCACCGG CTCATCAATG AGATATTTTC GGACGCTGTA 
GTACCGGATG TGCGTTCTGT AGTAACTACC CAACGTATGC AGGTATTGAA ACGTCAAGTA 
AGCTCACTAA CTATGCATCA GACTAAGCTT GAAGCTGAAC TACAACAAAT GGAAGAAAAG 
TTCGAGGCCA AAAAACAACG TATGGTTGAA TCTAGTGAGG CTTTCCAAGA AGAATTAAAG 
CGACACTGCA AACCTGCGGT AGATGATGAT ACATTCCAGA AAATGGTTGT TAAAATATAT 
GAAGAAATTA AGCGAGAACG TCAACGCGTA GATGAGCACC CCCAAGCTGC TATTTCTACT 
GCGACTGTAC CACAAGTAGT GAGTACTCCC AATGAAGAGA AAATCGTTTC TGCAACCGTG 
CAGCCTAACA CTGGTGTTAT GCCAGCTTCA GGGAAAAAAG ATTCGAATAT TGCAATTCCA 
AGCACACAAC CACTACAAGC TCCAAGAGAT GGGAATGAAT GCGGCAGTAA AACAGAACCG 
GAGCCAATGG ACATTGAACC AACCCCAAAA CCATCAGCAC CGTTACCCTG TAGGCCAGAG 
CTTCAAAAAT CTGCTGGTAA AACAGGAGTT GAATCAATAA AAGATGGTAA CAACAAATCA 
CCAACGTCAT CGAATATAAA ATCTGGACAA CCTGTTAAAG TACCAACTCC AACACCAACT 
CCACCTCCAC AATCGGGTCC CATAGAAAAC ACTACTACTA CAAATCATCC CCTACCATCT 
GCAACTTCAA CAAATCCACC ATCTCAGATT AGTGTGCCAC CAGCTCAAAC ACCACCACCA 
CAAGCTGTAA ACCAACCGAA TCAGCAACCA CCGCAACCAC AAACTACACA GCAACCGCCG 
CCAACCCAAC CAGTTCCATC AACGATGCCA ATGCATCCAC ATCATCCTCC TCCTCCAACA 
CATACACACT TACCACCTCA TTTACCGCCA CATCAATCCA TGTCAGCTAT GCCTCCTCAT 
GGCGGTTATG GCAATTACGG CGCCCCAACT GGACCTACTC GATCCCCGTA TTATCAACCG 
CAATACGGAG CGCATCCTGC TCAGCCCTAC GGTCAATATG CTCCTTATCC ACACTACCAA 
CAACCTTACG GTCCACCCCC AGGCTCACAT TATATGAATC CTCGACCACC ACCACAACAC 
AACGGTTCGC CTGTACATTA TCCCGAGCAT GGTGGACCTG TGTCAGCACA ACCTCAGCCA 
CATGATGTTT ATGGTCAACA ACCACCCCAT AACCCTGTTT TACCACAACA ACAACCAGTA 
ACTGGTACAG TCCCACCTTT AGCTGCTGCA GGATCAGGCG GCACCGGTGG CCCAACGCCT 
GCTTCTGGTA CCACGGTATC TGAGATTGGC AAAAGTGAGA TTGAAAAGAA AGATTCTGGC 
GCTGAGTAA