LOC105214662 cDNA ORF clone, Zeugodacus cucurbitae(melon fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC105214662
Entrez Gene ID	105214662
Full Name	titin
Gene Type	protein-coding
Organism	Zeugodacus cucurbitae(melon fly)

mRNA and Protein(s)

mRNA	Protein	Name
XM_011188213.1	XP_011186515.1	titin
XM_011188213.2	XP_011186515.1	titin homolog

Pathways from BioSystems
Gene Ontology

The following LOC105214662 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC105214662 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa192064	XM_011188213.2 Latest version!	Zeugodacus cucurbitae titin homolog (LOC105214662), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
View Old Accession Versions >>
OBa192064	XM_011188213.1	Bactrocera cucurbitae titin (LOC105214662), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC105214662 ( XM_011188213.1 ) cDNA ORF clone, Zeugodacus cucurbitae(melon fly) -> XP_011186515.1 Bactrocera cucurbitae titin (LOC105214662), mRNA.

Price & Availability↑

CloneID	OBa192064
Clone ID Related Accession (Same CDS sequence)	XM_011188213.1 , XM_011188213.2
Accession Version	XM_011188213.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3423bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-01-28
Organism	Zeugodacus cucurbitae(melon fly)
Product	titin
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011863666.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bactrocera cucurbitae Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGAAGC GCACTTTATT GTTAGTGTGC CTCAGTGGCT GCTGCCTGGC CGGACCGCTG 
CATGGAGTTA AGCGAAATGT GCCGATTGTG CAGCGCGAAG ATGATGTGGA GCGTCCCGCA 
CCAGAGCCAC TGGCGCCTGA GTACCCCGAA ATGCCGTCGA CACTGACCTT GCCCAGCAAT 
GCGACATCGA TACGCGCCGA CATCACCGAT AGCTTCTCGT GCGATGATAA AGTTTATGGT 
TATTATGCGG ACGTGGAGAA TGACTGCCAG ATATTCCACG TCTGTTTGCC GGTAACATAC 
GCTGACGGCA AAGAGAATAC ATTCCGTTGG AGTTTCATCT GCCCCGAAGA GACTGTATTC 
AGTCAGGAAT CATTCACCTG TATGCGTCGC GAAGATATTG CCTTCTCATG TGAAGACTCT 
GTAAACTATT ACGAATTGAA CCGCAATTTT GGTAATACCG AAGAGCAGAA CGCTAACTCC 
AACAATGCGC AAGAACAAGC GGAAGCACAA CCCGCGGAAA CAGCTGAGAC AGCCGAGCAA 
GAAGTGCAAC AAGCCGTTGC TCCACAAGCA GCTGCTCCAG CACCACAACC AGCTAAGCCA 
GTTAAACCAG TGAAGGTGCA GAAACCCAAT CGTAGGAAGC CACAACCCAA ACCACAACAA 
GACCAAGCTA TTTACCCCAC GAACAAATTG CCATCTCTTA TGCCACTACG CAAGATGCCC 
AATGTCAGCC CCATTGCGCC TGTATCCGTA CCCGCTGAAG TAGTGTCCGT ACAAGCAGTT 
GATGAGGACG AGGTAAAAGA AGTGCCATCC CGTTACAAGA AACGCCCAGC TGTTTCATAC 
AATAGCGAAA AAGAAGTCGT TGAAGTGCCT GCCCAACCTT TCAAAGTCGA GCTAGTACAT 
ACTGTCCACA ACCCGGAATC ATACAAACGC AGACGTCCCA CATTTTCGCA CAAGAATAAT 
GTTGTTACCA AAGTGACTGA AGATGTACCT GTCGCCGTTG AGACCGTACC CGAAGCTGTG 
GAAGTTGTGG AACCTGTTGT GCCAGCATTG AAATTCGAAT CAAACTTGGG CGCTAGTTCA 
GTACAGGCAC AGGAAGAAAT TGAAGCTGTT GCAGAACCTG TAGAATCGGT TGCCGTAGAA 
GTACCAGCTG TTGCTGAAGC TGAAATTCCA GCCGTAGTCG ATGCAGAACC ACAAGCTTCT 
GAAGCTGGTG AAGTTGCTGT GGAGCCAGAA CAGACTTTGA AAACAATCGC CATACAACCA 
CTTGTTCCAG AAGCTGAAGT TGCACAAGCG GAGGAAGCTT TTGTGGCGCC AATTGAGTTA 
GCGCAACCAG TTGTGGAAGC CGAACCCGAA TATGTAGCAC CAATTGTAGA GGAAATTAAA 
ATTGAAGAAC CTGTTGCTGA GGAAGTTTCA AACGTAGAAG TAGCACCAGC GGAAGAAGTG 
ACAAAAGTTG AGGAACCAAT TGTGGCTGAG TTGCCAGTAG CAGTTGAAGC CGAACCAGAG 
AAGGAAGCAC AAAATGAAGA ACTAGCTGTT GAAGCAATTG AACCAGCTCC TATTTCTGAT 
GCTGTTGTGG AAGTACCAGC TGAACAACAA ATCAATGTAG ACACACCAGC ACAGTCTGAA 
GAACAAGCTC AACCCGAACA ACTTGCCGAA AACGCCGAGG ATCAATTAGT TGGCGCATCT 
GAACCAGTTG CTTTGGAAGC TGAGCCCGCA GTTATTGCTC CAGTTGAAAA TACAGAAGAA 
TCTTTGCCTG CTCTCCCCTC ACTTGAGGAG TTGGAAGAAC AGAAGCCAGC CGATGCTGTT 
GAAACCATGC CTGCTACACC TGAAATGGAA CAACAAGATC CCATTGTGCA ACAAGCTTTC 
GAAGACAATG ACGCTCACTT GCAGACTGTT GGTGGTTTCA AACCCGTAGA CCCTGAAGTC 
GCTGCTGAAG CTGAAGCACT TATTTCAGAC TTCATCAATA CCCTAAAGAG CGCTCACCCA 
CATGAGAAAC CCGTCCTACC TGTGCAGCTG GAAGAGGAAG CACCAGTAGC TTCTGCTGAA 
AGTGAAGATG CTAAAATTGA AGTAGAAGCA CCACAACAGA GTGAAGAGAT AATTGAAGCA 
GTAGAAGCAC AGCCAGAAGA AGTACCCGTT GAAGCTGAAG CTGAAGCTGA AGCTGAAGCT 
GAAGCTGAAG CCAACTTTTT GAAAACCGTA GCTGCTGAAG AACCATCAGC GGTTGAAGCT 
GAAAGTGAAC CAATTGTAGC TGAAGATGTG CCAGTCGCTG AAGAGGAAGT GAAAGTTTCA 
CCACTGCTTA GATTCCCCAT CTCCAACAGT TACCCCGCCA ACATGTATCA GATTCCAGTA 
ACAGTCATTC AAACACCCTA CGAAGAGGTA CAAAACGCCG AAGCGGAAGT ACAAGAACCA 
GAGCAAGAAA TCAATGCTGA AGAAGTAAAA GCTGAAGCAG TACAACCTGT AGAAGAAGTT 
GTGCCAGCTG TAGCAGAAAC TCAAGAGAAA TTGAAATTCT TGCCCGAACA AACTGTAGCA 
GAAATTACAG AAGAACAAAA AGCAGAGGAT ATTCCAGCTG TAGAAAGCCA GCCAGAAGCT 
CAACCCGCTG TTGCTGAGTT GGTGGAAACC GAACCTGTTG TCGCTGAAGC CGTCGAGCCT 
GCACTAGAAG CTGTACAACC AGTTCTTGCC GAAGCTGTAG AGAGCGCGCC AGAAGCTGAA 
CCAGAAGTTG CACAACCCGT CGCTCCCGTC GCCTACAGAC CCATCAGCAT CGATGACATC 
GTTGAATTGG TGAAAGAGCG TTTAGATCAA GCGCCCGCCG ATGAGAAAGG TGCACCCATG 
GAACTGGTTG TAGACTCCAG CAACGCCGAT GCGCCCGTTG AATTGAAATT TAGTTTGGAA 
AAAGGGTCAG CGCCGCAAGA AGTATCAGCT GAACAAGCAC CTGCCGAGGA TATCATTTTT 
CCCATTTATC ATCGAGTGTC CGAGCCTGAG GTCAGCGCTG TACAAGCACC AGAAGCCAGC 
GCATTCGTAA CGGAGCTCAA AAAGTTACCC GTTGAAGCTG AGGCCGTACA AGACGTAGTC 
GAACCGCTAG TGCAAACTGT AGACCAGGCT GTGCAAACCG ATGCCGACGA ACTTGAAGCT 
AATGTCGCTC TCAAGTCCGC AATCAACGCC GAGACTGAGC AGGAGGCTGA AGCTGTGCCA 
GCGCCAGTTG AGACTGTGCA AGAAGTTGAA AGTGAAGCCG AAGCTGTAGC AGCACCAGTT 
GAGAATGTGC AAGAAGCTGA GAGTGAAGCT GCTGTTGTGG CAGCTGATGC GCCCGTTGAG 
ACCGCAGCTG TTGAAGCTGA TGAAAGTGCG AAGGCAAATC GTGCCTTGAG ATATCGTAGC 
TTGAACAAAG CTAAGTTAGA TCCACGTAAA CGTCGCTTCC TCTTCAGATC GGATGCCAGT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011186515.1
CDS	67..3489
Translation	MWKRTLLLVCLSGCCLAGPLHGVKRNVPIVQREDDVERPAPEPLAPEYPEMPSTLTLPSNATSIRADITDSFSCDDKVYGYYADVENDCQIFHVCLPVTYADGKENTFRWSFICPEETVFSQESFTCMRREDIAFSCEDSVNYYELNRNFGNTEEQNANSNNAQEQAEAQPAETAETAEQEVQQAVAPQAAAPAPQPAKPVKPVKVQKPNRRKPQPKPQQDQAIYPTNKLPSLMPLRKMPNVSPIAPVSVPAEVVSVQAVDEDEVKEVPSRYKKRPAVSYNSEKEVVEVPAQPFKVELVHTVHNPESYKRRRPTFSHKNNVVTKVTEDVPVAVETVPEAVEVVEPVVPALKFESNLGASSVQAQEEIEAVAEPVESVAVEVPAVAEAEIPAVVDAEPQASEAGEVAVEPEQTLKTIAIQPLVPEAEVAQAEEAFVAPIELAQPVVEAEPEYVAPIVEEIKIEEPVAEEVSNVEVAPAEEVTKVEEPIVAELPVAVEAEPEKEAQNEELAVEAIEPAPISDAVVEVPAEQQINVDTPAQSEEQAQPEQLAENAEDQLVGASEPVALEAEPAVIAPVENTEESLPALPSLEELEEQKPADAVETMPATPEMEQQDPIVQQAFEDNDAHLQTVGGFKPVDPEVAAEAEALISDFINTLKSAHPHEKPVLPVQLEEEAPVASAESEDAKIEVEAPQQSEEIIEAVEAQPEEVPVEAEAEAEAEAEAEANFLKTVAAEEPSAVEAESEPIVAEDVPVAEEEVKVSPLLRFPISNSYPANMYQIPVTVIQTPYEEVQNAEAEVQEPEQEINAEEVKAEAVQPVEEVVPAVAETQEKLKFLPEQTVAEITEEQKAEDIPAVESQPEAQPAVAELVETEPVVAEAVEPALEAVQPVLAEAVESAPEAEPEVAQPVAPVAYRPISIDDIVELVKERLDQAPADEKGAPMELVVDSSNADAPVELKFSLEKGSAPQEVSAEQAPAEDIIFPIYHRVSEPEVSAVQAPEASAFVTELKKLPVEAEAVQDVVEPLVQTVDQAVQTDADELEANVALKSAINAETEQEAEAVPAPVETVQEVESEAEAVAAPVENVQEAESEAAVVAADAPVETAAVEADESAKANRALRYRSLNKAKLDPRKRRFLFRSDAS

Target ORF information:

RefSeq Version	XM_011188213.1
Organism	Zeugodacus cucurbitae(melon fly)
Definition	Bactrocera cucurbitae titin (LOC105214662), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011188213.1

ORF Insert Sequence:

ATGTGGAAGC GCACTTTATT GTTAGTGTGC CTCAGTGGCT GCTGCCTGGC CGGACCGCTG 
CATGGAGTTA AGCGAAATGT GCCGATTGTG CAGCGCGAAG ATGATGTGGA GCGTCCCGCA 
CCAGAGCCAC TGGCGCCTGA GTACCCCGAA ATGCCGTCGA CACTGACCTT GCCCAGCAAT 
GCGACATCGA TACGCGCCGA CATCACCGAT AGCTTCTCGT GCGATGATAA AGTTTATGGT 
TATTATGCGG ACGTGGAGAA TGACTGCCAG ATATTCCACG TCTGTTTGCC GGTAACATAC 
GCTGACGGCA AAGAGAATAC ATTCCGTTGG AGTTTCATCT GCCCCGAAGA GACTGTATTC 
AGTCAGGAAT CATTCACCTG TATGCGTCGC GAAGATATTG CCTTCTCATG TGAAGACTCT 
GTAAACTATT ACGAATTGAA CCGCAATTTT GGTAATACCG AAGAGCAGAA CGCTAACTCC 
AACAATGCGC AAGAACAAGC GGAAGCACAA CCCGCGGAAA CAGCTGAGAC AGCCGAGCAA 
GAAGTGCAAC AAGCCGTTGC TCCACAAGCA GCTGCTCCAG CACCACAACC AGCTAAGCCA 
GTTAAACCAG TGAAGGTGCA GAAACCCAAT CGTAGGAAGC CACAACCCAA ACCACAACAA 
GACCAAGCTA TTTACCCCAC GAACAAATTG CCATCTCTTA TGCCACTACG CAAGATGCCC 
AATGTCAGCC CCATTGCGCC TGTATCCGTA CCCGCTGAAG TAGTGTCCGT ACAAGCAGTT 
GATGAGGACG AGGTAAAAGA AGTGCCATCC CGTTACAAGA AACGCCCAGC TGTTTCATAC 
AATAGCGAAA AAGAAGTCGT TGAAGTGCCT GCCCAACCTT TCAAAGTCGA GCTAGTACAT 
ACTGTCCACA ACCCGGAATC ATACAAACGC AGACGTCCCA CATTTTCGCA CAAGAATAAT 
GTTGTTACCA AAGTGACTGA AGATGTACCT GTCGCCGTTG AGACCGTACC CGAAGCTGTG 
GAAGTTGTGG AACCTGTTGT GCCAGCATTG AAATTCGAAT CAAACTTGGG CGCTAGTTCA 
GTACAGGCAC AGGAAGAAAT TGAAGCTGTT GCAGAACCTG TAGAATCGGT TGCCGTAGAA 
GTACCAGCTG TTGCTGAAGC TGAAATTCCA GCCGTAGTCG ATGCAGAACC ACAAGCTTCT 
GAAGCTGGTG AAGTTGCTGT GGAGCCAGAA CAGACTTTGA AAACAATCGC CATACAACCA 
CTTGTTCCAG AAGCTGAAGT TGCACAAGCG GAGGAAGCTT TTGTGGCGCC AATTGAGTTA 
GCGCAACCAG TTGTGGAAGC CGAACCCGAA TATGTAGCAC CAATTGTAGA GGAAATTAAA 
ATTGAAGAAC CTGTTGCTGA GGAAGTTTCA AACGTAGAAG TAGCACCAGC GGAAGAAGTG 
ACAAAAGTTG AGGAACCAAT TGTGGCTGAG TTGCCAGTAG CAGTTGAAGC CGAACCAGAG 
AAGGAAGCAC AAAATGAAGA ACTAGCTGTT GAAGCAATTG AACCAGCTCC TATTTCTGAT 
GCTGTTGTGG AAGTACCAGC TGAACAACAA ATCAATGTAG ACACACCAGC ACAGTCTGAA 
GAACAAGCTC AACCCGAACA ACTTGCCGAA AACGCCGAGG ATCAATTAGT TGGCGCATCT 
GAACCAGTTG CTTTGGAAGC TGAGCCCGCA GTTATTGCTC CAGTTGAAAA TACAGAAGAA 
TCTTTGCCTG CTCTCCCCTC ACTTGAGGAG TTGGAAGAAC AGAAGCCAGC CGATGCTGTT 
GAAACCATGC CTGCTACACC TGAAATGGAA CAACAAGATC CCATTGTGCA ACAAGCTTTC 
GAAGACAATG ACGCTCACTT GCAGACTGTT GGTGGTTTCA AACCCGTAGA CCCTGAAGTC 
GCTGCTGAAG CTGAAGCACT TATTTCAGAC TTCATCAATA CCCTAAAGAG CGCTCACCCA 
CATGAGAAAC CCGTCCTACC TGTGCAGCTG GAAGAGGAAG CACCAGTAGC TTCTGCTGAA 
AGTGAAGATG CTAAAATTGA AGTAGAAGCA CCACAACAGA GTGAAGAGAT AATTGAAGCA 
GTAGAAGCAC AGCCAGAAGA AGTACCCGTT GAAGCTGAAG CTGAAGCTGA AGCTGAAGCT 
GAAGCTGAAG CCAACTTTTT GAAAACCGTA GCTGCTGAAG AACCATCAGC GGTTGAAGCT 
GAAAGTGAAC CAATTGTAGC TGAAGATGTG CCAGTCGCTG AAGAGGAAGT GAAAGTTTCA 
CCACTGCTTA GATTCCCCAT CTCCAACAGT TACCCCGCCA ACATGTATCA GATTCCAGTA 
ACAGTCATTC AAACACCCTA CGAAGAGGTA CAAAACGCCG AAGCGGAAGT ACAAGAACCA 
GAGCAAGAAA TCAATGCTGA AGAAGTAAAA GCTGAAGCAG TACAACCTGT AGAAGAAGTT 
GTGCCAGCTG TAGCAGAAAC TCAAGAGAAA TTGAAATTCT TGCCCGAACA AACTGTAGCA 
GAAATTACAG AAGAACAAAA AGCAGAGGAT ATTCCAGCTG TAGAAAGCCA GCCAGAAGCT 
CAACCCGCTG TTGCTGAGTT GGTGGAAACC GAACCTGTTG TCGCTGAAGC CGTCGAGCCT 
GCACTAGAAG CTGTACAACC AGTTCTTGCC GAAGCTGTAG AGAGCGCGCC AGAAGCTGAA 
CCAGAAGTTG CACAACCCGT CGCTCCCGTC GCCTACAGAC CCATCAGCAT CGATGACATC 
GTTGAATTGG TGAAAGAGCG TTTAGATCAA GCGCCCGCCG ATGAGAAAGG TGCACCCATG 
GAACTGGTTG TAGACTCCAG CAACGCCGAT GCGCCCGTTG AATTGAAATT TAGTTTGGAA 
AAAGGGTCAG CGCCGCAAGA AGTATCAGCT GAACAAGCAC CTGCCGAGGA TATCATTTTT 
CCCATTTATC ATCGAGTGTC CGAGCCTGAG GTCAGCGCTG TACAAGCACC AGAAGCCAGC 
GCATTCGTAA CGGAGCTCAA AAAGTTACCC GTTGAAGCTG AGGCCGTACA AGACGTAGTC 
GAACCGCTAG TGCAAACTGT AGACCAGGCT GTGCAAACCG ATGCCGACGA ACTTGAAGCT 
AATGTCGCTC TCAAGTCCGC AATCAACGCC GAGACTGAGC AGGAGGCTGA AGCTGTGCCA 
GCGCCAGTTG AGACTGTGCA AGAAGTTGAA AGTGAAGCCG AAGCTGTAGC AGCACCAGTT 
GAGAATGTGC AAGAAGCTGA GAGTGAAGCT GCTGTTGTGG CAGCTGATGC GCCCGTTGAG 
ACCGCAGCTG TTGAAGCTGA TGAAAGTGCG AAGGCAAATC GTGCCTTGAG ATATCGTAGC 
TTGAACAAAG CTAAGTTAGA TCCACGTAAA CGTCGCTTCC TCTTCAGATC GGATGCCAGT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC105214662 ( XM_011188213.2 ) cDNA ORF clone, Zeugodacus cucurbitae(melon fly) -> XP_011186515.1 Zeugodacus cucurbitae titin homolog (LOC105214662), mRNA.

Price & Availability↑

CloneID	OBa192064
Clone ID Related Accession (Same CDS sequence)	XM_011188213.1 , XM_011188213.2
Accession Version	XM_011188213.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3423bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-27
Organism	Zeugodacus cucurbitae(melon fly)
Product	titin homolog
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011863666.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 28, 2019 this sequence version replaced XM_011188213.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Zeugodacus cucurbitae Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGAAGC GCACTTTATT GTTAGTGTGC CTCAGTGGCT GCTGCCTGGC CGGACCGCTG 
CATGGAGTTA AGCGAAATGT GCCGATTGTG CAGCGCGAAG ATGATGTGGA GCGTCCCGCA 
CCAGAGCCAC TGGCGCCTGA GTACCCCGAA ATGCCGTCGA CACTGACCTT GCCCAGCAAT 
GCGACATCGA TACGCGCCGA CATCACCGAT AGCTTCTCGT GCGATGATAA AGTTTATGGT 
TATTATGCGG ACGTGGAGAA TGACTGCCAG ATATTCCACG TCTGTTTGCC GGTAACATAC 
GCTGACGGCA AAGAGAATAC ATTCCGTTGG AGTTTCATCT GCCCCGAAGA GACTGTATTC 
AGTCAGGAAT CATTCACCTG TATGCGTCGC GAAGATATTG CCTTCTCATG TGAAGACTCT 
GTAAACTATT ACGAATTGAA CCGCAATTTT GGTAATACCG AAGAGCAGAA CGCTAACTCC 
AACAATGCGC AAGAACAAGC GGAAGCACAA CCCGCGGAAA CAGCTGAGAC AGCCGAGCAA 
GAAGTGCAAC AAGCCGTTGC TCCACAAGCA GCTGCTCCAG CACCACAACC AGCTAAGCCA 
GTTAAACCAG TGAAGGTGCA GAAACCCAAT CGTAGGAAGC CACAACCCAA ACCACAACAA 
GACCAAGCTA TTTACCCCAC GAACAAATTG CCATCTCTTA TGCCACTACG CAAGATGCCC 
AATGTCAGCC CCATTGCGCC TGTATCCGTA CCCGCTGAAG TAGTGTCCGT ACAAGCAGTT 
GATGAGGACG AGGTAAAAGA AGTGCCATCC CGTTACAAGA AACGCCCAGC TGTTTCATAC 
AATAGCGAAA AAGAAGTCGT TGAAGTGCCT GCCCAACCTT TCAAAGTCGA GCTAGTACAT 
ACTGTCCACA ACCCGGAATC ATACAAACGC AGACGTCCCA CATTTTCGCA CAAGAATAAT 
GTTGTTACCA AAGTGACTGA AGATGTACCT GTCGCCGTTG AGACCGTACC CGAAGCTGTG 
GAAGTTGTGG AACCTGTTGT GCCAGCATTG AAATTCGAAT CAAACTTGGG CGCTAGTTCA 
GTACAGGCAC AGGAAGAAAT TGAAGCTGTT GCAGAACCTG TAGAATCGGT TGCCGTAGAA 
GTACCAGCTG TTGCTGAAGC TGAAATTCCA GCCGTAGTCG ATGCAGAACC ACAAGCTTCT 
GAAGCTGGTG AAGTTGCTGT GGAGCCAGAA CAGACTTTGA AAACAATCGC CATACAACCA 
CTTGTTCCAG AAGCTGAAGT TGCACAAGCG GAGGAAGCTT TTGTGGCGCC AATTGAGTTA 
GCGCAACCAG TTGTGGAAGC CGAACCCGAA TATGTAGCAC CAATTGTAGA GGAAATTAAA 
ATTGAAGAAC CTGTTGCTGA GGAAGTTTCA AACGTAGAAG TAGCACCAGC GGAAGAAGTG 
ACAAAAGTTG AGGAACCAAT TGTGGCTGAG TTGCCAGTAG CAGTTGAAGC CGAACCAGAG 
AAGGAAGCAC AAAATGAAGA ACTAGCTGTT GAAGCAATTG AACCAGCTCC TATTTCTGAT 
GCTGTTGTGG AAGTACCAGC TGAACAACAA ATCAATGTAG ACACACCAGC ACAGTCTGAA 
GAACAAGCTC AACCCGAACA ACTTGCCGAA AACGCCGAGG ATCAATTAGT TGGCGCATCT 
GAACCAGTTG CTTTGGAAGC TGAGCCCGCA GTTATTGCTC CAGTTGAAAA TACAGAAGAA 
TCTTTGCCTG CTCTCCCCTC ACTTGAGGAG TTGGAAGAAC AGAAGCCAGC CGATGCTGTT 
GAAACCATGC CTGCTACACC TGAAATGGAA CAACAAGATC CCATTGTGCA ACAAGCTTTC 
GAAGACAATG ACGCTCACTT GCAGACTGTT GGTGGTTTCA AACCCGTAGA CCCTGAAGTC 
GCTGCTGAAG CTGAAGCACT TATTTCAGAC TTCATCAATA CCCTAAAGAG CGCTCACCCA 
CATGAGAAAC CCGTCCTACC TGTGCAGCTG GAAGAGGAAG CACCAGTAGC TTCTGCTGAA 
AGTGAAGATG CTAAAATTGA AGTAGAAGCA CCACAACAGA GTGAAGAGAT AATTGAAGCA 
GTAGAAGCAC AGCCAGAAGA AGTACCCGTT GAAGCTGAAG CTGAAGCTGA AGCTGAAGCT 
GAAGCTGAAG CCAACTTTTT GAAAACCGTA GCTGCTGAAG AACCATCAGC GGTTGAAGCT 
GAAAGTGAAC CAATTGTAGC TGAAGATGTG CCAGTCGCTG AAGAGGAAGT GAAAGTTTCA 
CCACTGCTTA GATTCCCCAT CTCCAACAGT TACCCCGCCA ACATGTATCA GATTCCAGTA 
ACAGTCATTC AAACACCCTA CGAAGAGGTA CAAAACGCCG AAGCGGAAGT ACAAGAACCA 
GAGCAAGAAA TCAATGCTGA AGAAGTAAAA GCTGAAGCAG TACAACCTGT AGAAGAAGTT 
GTGCCAGCTG TAGCAGAAAC TCAAGAGAAA TTGAAATTCT TGCCCGAACA AACTGTAGCA 
GAAATTACAG AAGAACAAAA AGCAGAGGAT ATTCCAGCTG TAGAAAGCCA GCCAGAAGCT 
CAACCCGCTG TTGCTGAGTT GGTGGAAACC GAACCTGTTG TCGCTGAAGC CGTCGAGCCT 
GCACTAGAAG CTGTACAACC AGTTCTTGCC GAAGCTGTAG AGAGCGCGCC AGAAGCTGAA 
CCAGAAGTTG CACAACCCGT CGCTCCCGTC GCCTACAGAC CCATCAGCAT CGATGACATC 
GTTGAATTGG TGAAAGAGCG TTTAGATCAA GCGCCCGCCG ATGAGAAAGG TGCACCCATG 
GAACTGGTTG TAGACTCCAG CAACGCCGAT GCGCCCGTTG AATTGAAATT TAGTTTGGAA 
AAAGGGTCAG CGCCGCAAGA AGTATCAGCT GAACAAGCAC CTGCCGAGGA TATCATTTTT 
CCCATTTATC ATCGAGTGTC CGAGCCTGAG GTCAGCGCTG TACAAGCACC AGAAGCCAGC 
GCATTCGTAA CGGAGCTCAA AAAGTTACCC GTTGAAGCTG AGGCCGTACA AGACGTAGTC 
GAACCGCTAG TGCAAACTGT AGACCAGGCT GTGCAAACCG ATGCCGACGA ACTTGAAGCT 
AATGTCGCTC TCAAGTCCGC AATCAACGCC GAGACTGAGC AGGAGGCTGA AGCTGTGCCA 
GCGCCAGTTG AGACTGTGCA AGAAGTTGAA AGTGAAGCCG AAGCTGTAGC AGCACCAGTT 
GAGAATGTGC AAGAAGCTGA GAGTGAAGCT GCTGTTGTGG CAGCTGATGC GCCCGTTGAG 
ACCGCAGCTG TTGAAGCTGA TGAAAGTGCG AAGGCAAATC GTGCCTTGAG ATATCGTAGC 
TTGAACAAAG CTAAGTTAGA TCCACGTAAA CGTCGCTTCC TCTTCAGATC GGATGCCAGT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011186515.1
CDS	75..3497
Translation	MWKRTLLLVCLSGCCLAGPLHGVKRNVPIVQREDDVERPAPEPLAPEYPEMPSTLTLPSNATSIRADITDSFSCDDKVYGYYADVENDCQIFHVCLPVTYADGKENTFRWSFICPEETVFSQESFTCMRREDIAFSCEDSVNYYELNRNFGNTEEQNANSNNAQEQAEAQPAETAETAEQEVQQAVAPQAAAPAPQPAKPVKPVKVQKPNRRKPQPKPQQDQAIYPTNKLPSLMPLRKMPNVSPIAPVSVPAEVVSVQAVDEDEVKEVPSRYKKRPAVSYNSEKEVVEVPAQPFKVELVHTVHNPESYKRRRPTFSHKNNVVTKVTEDVPVAVETVPEAVEVVEPVVPALKFESNLGASSVQAQEEIEAVAEPVESVAVEVPAVAEAEIPAVVDAEPQASEAGEVAVEPEQTLKTIAIQPLVPEAEVAQAEEAFVAPIELAQPVVEAEPEYVAPIVEEIKIEEPVAEEVSNVEVAPAEEVTKVEEPIVAELPVAVEAEPEKEAQNEELAVEAIEPAPISDAVVEVPAEQQINVDTPAQSEEQAQPEQLAENAEDQLVGASEPVALEAEPAVIAPVENTEESLPALPSLEELEEQKPADAVETMPATPEMEQQDPIVQQAFEDNDAHLQTVGGFKPVDPEVAAEAEALISDFINTLKSAHPHEKPVLPVQLEEEAPVASAESEDAKIEVEAPQQSEEIIEAVEAQPEEVPVEAEAEAEAEAEAEANFLKTVAAEEPSAVEAESEPIVAEDVPVAEEEVKVSPLLRFPISNSYPANMYQIPVTVIQTPYEEVQNAEAEVQEPEQEINAEEVKAEAVQPVEEVVPAVAETQEKLKFLPEQTVAEITEEQKAEDIPAVESQPEAQPAVAELVETEPVVAEAVEPALEAVQPVLAEAVESAPEAEPEVAQPVAPVAYRPISIDDIVELVKERLDQAPADEKGAPMELVVDSSNADAPVELKFSLEKGSAPQEVSAEQAPAEDIIFPIYHRVSEPEVSAVQAPEASAFVTELKKLPVEAEAVQDVVEPLVQTVDQAVQTDADELEANVALKSAINAETEQEAEAVPAPVETVQEVESEAEAVAAPVENVQEAESEAAVVAADAPVETAAVEADESAKANRALRYRSLNKAKLDPRKRRFLFRSDAS

Target ORF information:

RefSeq Version	XM_011188213.2
Organism	Zeugodacus cucurbitae(melon fly)
Definition	Zeugodacus cucurbitae titin homolog (LOC105214662), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011188213.2

ORF Insert Sequence:

ATGTGGAAGC GCACTTTATT GTTAGTGTGC CTCAGTGGCT GCTGCCTGGC CGGACCGCTG 
CATGGAGTTA AGCGAAATGT GCCGATTGTG CAGCGCGAAG ATGATGTGGA GCGTCCCGCA 
CCAGAGCCAC TGGCGCCTGA GTACCCCGAA ATGCCGTCGA CACTGACCTT GCCCAGCAAT 
GCGACATCGA TACGCGCCGA CATCACCGAT AGCTTCTCGT GCGATGATAA AGTTTATGGT 
TATTATGCGG ACGTGGAGAA TGACTGCCAG ATATTCCACG TCTGTTTGCC GGTAACATAC 
GCTGACGGCA AAGAGAATAC ATTCCGTTGG AGTTTCATCT GCCCCGAAGA GACTGTATTC 
AGTCAGGAAT CATTCACCTG TATGCGTCGC GAAGATATTG CCTTCTCATG TGAAGACTCT 
GTAAACTATT ACGAATTGAA CCGCAATTTT GGTAATACCG AAGAGCAGAA CGCTAACTCC 
AACAATGCGC AAGAACAAGC GGAAGCACAA CCCGCGGAAA CAGCTGAGAC AGCCGAGCAA 
GAAGTGCAAC AAGCCGTTGC TCCACAAGCA GCTGCTCCAG CACCACAACC AGCTAAGCCA 
GTTAAACCAG TGAAGGTGCA GAAACCCAAT CGTAGGAAGC CACAACCCAA ACCACAACAA 
GACCAAGCTA TTTACCCCAC GAACAAATTG CCATCTCTTA TGCCACTACG CAAGATGCCC 
AATGTCAGCC CCATTGCGCC TGTATCCGTA CCCGCTGAAG TAGTGTCCGT ACAAGCAGTT 
GATGAGGACG AGGTAAAAGA AGTGCCATCC CGTTACAAGA AACGCCCAGC TGTTTCATAC 
AATAGCGAAA AAGAAGTCGT TGAAGTGCCT GCCCAACCTT TCAAAGTCGA GCTAGTACAT 
ACTGTCCACA ACCCGGAATC ATACAAACGC AGACGTCCCA CATTTTCGCA CAAGAATAAT 
GTTGTTACCA AAGTGACTGA AGATGTACCT GTCGCCGTTG AGACCGTACC CGAAGCTGTG 
GAAGTTGTGG AACCTGTTGT GCCAGCATTG AAATTCGAAT CAAACTTGGG CGCTAGTTCA 
GTACAGGCAC AGGAAGAAAT TGAAGCTGTT GCAGAACCTG TAGAATCGGT TGCCGTAGAA 
GTACCAGCTG TTGCTGAAGC TGAAATTCCA GCCGTAGTCG ATGCAGAACC ACAAGCTTCT 
GAAGCTGGTG AAGTTGCTGT GGAGCCAGAA CAGACTTTGA AAACAATCGC CATACAACCA 
CTTGTTCCAG AAGCTGAAGT TGCACAAGCG GAGGAAGCTT TTGTGGCGCC AATTGAGTTA 
GCGCAACCAG TTGTGGAAGC CGAACCCGAA TATGTAGCAC CAATTGTAGA GGAAATTAAA 
ATTGAAGAAC CTGTTGCTGA GGAAGTTTCA AACGTAGAAG TAGCACCAGC GGAAGAAGTG 
ACAAAAGTTG AGGAACCAAT TGTGGCTGAG TTGCCAGTAG CAGTTGAAGC CGAACCAGAG 
AAGGAAGCAC AAAATGAAGA ACTAGCTGTT GAAGCAATTG AACCAGCTCC TATTTCTGAT 
GCTGTTGTGG AAGTACCAGC TGAACAACAA ATCAATGTAG ACACACCAGC ACAGTCTGAA 
GAACAAGCTC AACCCGAACA ACTTGCCGAA AACGCCGAGG ATCAATTAGT TGGCGCATCT 
GAACCAGTTG CTTTGGAAGC TGAGCCCGCA GTTATTGCTC CAGTTGAAAA TACAGAAGAA 
TCTTTGCCTG CTCTCCCCTC ACTTGAGGAG TTGGAAGAAC AGAAGCCAGC CGATGCTGTT 
GAAACCATGC CTGCTACACC TGAAATGGAA CAACAAGATC CCATTGTGCA ACAAGCTTTC 
GAAGACAATG ACGCTCACTT GCAGACTGTT GGTGGTTTCA AACCCGTAGA CCCTGAAGTC 
GCTGCTGAAG CTGAAGCACT TATTTCAGAC TTCATCAATA CCCTAAAGAG CGCTCACCCA 
CATGAGAAAC CCGTCCTACC TGTGCAGCTG GAAGAGGAAG CACCAGTAGC TTCTGCTGAA 
AGTGAAGATG CTAAAATTGA AGTAGAAGCA CCACAACAGA GTGAAGAGAT AATTGAAGCA 
GTAGAAGCAC AGCCAGAAGA AGTACCCGTT GAAGCTGAAG CTGAAGCTGA AGCTGAAGCT 
GAAGCTGAAG CCAACTTTTT GAAAACCGTA GCTGCTGAAG AACCATCAGC GGTTGAAGCT 
GAAAGTGAAC CAATTGTAGC TGAAGATGTG CCAGTCGCTG AAGAGGAAGT GAAAGTTTCA 
CCACTGCTTA GATTCCCCAT CTCCAACAGT TACCCCGCCA ACATGTATCA GATTCCAGTA 
ACAGTCATTC AAACACCCTA CGAAGAGGTA CAAAACGCCG AAGCGGAAGT ACAAGAACCA 
GAGCAAGAAA TCAATGCTGA AGAAGTAAAA GCTGAAGCAG TACAACCTGT AGAAGAAGTT 
GTGCCAGCTG TAGCAGAAAC TCAAGAGAAA TTGAAATTCT TGCCCGAACA AACTGTAGCA 
GAAATTACAG AAGAACAAAA AGCAGAGGAT ATTCCAGCTG TAGAAAGCCA GCCAGAAGCT 
CAACCCGCTG TTGCTGAGTT GGTGGAAACC GAACCTGTTG TCGCTGAAGC CGTCGAGCCT 
GCACTAGAAG CTGTACAACC AGTTCTTGCC GAAGCTGTAG AGAGCGCGCC AGAAGCTGAA 
CCAGAAGTTG CACAACCCGT CGCTCCCGTC GCCTACAGAC CCATCAGCAT CGATGACATC 
GTTGAATTGG TGAAAGAGCG TTTAGATCAA GCGCCCGCCG ATGAGAAAGG TGCACCCATG 
GAACTGGTTG TAGACTCCAG CAACGCCGAT GCGCCCGTTG AATTGAAATT TAGTTTGGAA 
AAAGGGTCAG CGCCGCAAGA AGTATCAGCT GAACAAGCAC CTGCCGAGGA TATCATTTTT 
CCCATTTATC ATCGAGTGTC CGAGCCTGAG GTCAGCGCTG TACAAGCACC AGAAGCCAGC 
GCATTCGTAA CGGAGCTCAA AAAGTTACCC GTTGAAGCTG AGGCCGTACA AGACGTAGTC 
GAACCGCTAG TGCAAACTGT AGACCAGGCT GTGCAAACCG ATGCCGACGA ACTTGAAGCT 
AATGTCGCTC TCAAGTCCGC AATCAACGCC GAGACTGAGC AGGAGGCTGA AGCTGTGCCA 
GCGCCAGTTG AGACTGTGCA AGAAGTTGAA AGTGAAGCCG AAGCTGTAGC AGCACCAGTT 
GAGAATGTGC AAGAAGCTGA GAGTGAAGCT GCTGTTGTGG CAGCTGATGC GCCCGTTGAG 
ACCGCAGCTG TTGAAGCTGA TGAAAGTGCG AAGGCAAATC GTGCCTTGAG ATATCGTAGC 
TTGAACAAAG CTAAGTTAGA TCCACGTAAA CGTCGCTTCC TCTTCAGATC GGATGCCAGT 
TAG