LOC105211875 cDNA ORF clone, Zeugodacus cucurbitae(melon fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC105211875
Entrez Gene ID	105211875
Full Name	M-phase inducer phosphatase
Gene Type	protein-coding
Organism	Zeugodacus cucurbitae(melon fly)

mRNA and Protein(s)

mRNA	Protein	Name
XM_011183545.2	XP_011181847.1	M-phase inducer phosphatase

Pathways from BioSystems
Gene Ontology

The following LOC105211875 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC105211875 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBa197971	XM_011183545.2 Latest version!	Zeugodacus cucurbitae M-phase inducer phosphatase (LOC105211875), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC105211875 ( XM_011183545.2 ) cDNA ORF clone, Zeugodacus cucurbitae(melon fly) -> XP_011181847.1 Zeugodacus cucurbitae M-phase inducer phosphatase (LOC105211875), mRNA.

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CloneID	OBa197971
Clone ID Related Accession (Same CDS sequence)	XM_011183545.2
Accession Version	XM_011183545.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1668bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-27
Organism	Zeugodacus cucurbitae(melon fly)
Product	M-phase inducer phosphatase
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011863698.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 28, 2019 this sequence version replaced XM_011183545.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Zeugodacus cucurbitae Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTGGG ACACAATTGC CGAAGAAACC AACGGAGAAA TGGCCATGGA ATTTGCAAGC 
TGTCAGATGC CACACAACTT GAATTCTGCC GCTGCTAGCA GTAATGGAGC CAGCCGTCAA 
CGCCAGTCGT TGAACATCTT GAACAGCTCG ATGAACGACA TGGCAGTCGA TCATCAGTTG 
TCAATCTATG GCGATGACGA ACATGCGGAG CTGAATCAAG TCGAAAACTT GCGTTCGGCC 
GATTTCGATA TGCCACAACA GATGGGTTTG CTGTCACCTG AAGGCTCACC ATGCCGTTTC 
CAAATAGTAC AAAAACCAAG AGCATCGAAT GGTCAACAGC GCCAGTCATT TGCATCACGC 
CTACAGAAGC TACAACATAA CAATACCGGC AATGGCAGTG CTAGCACACC AACACCACCG 
AAGAGTCTGC GTATTTTCCA CAGCTTGTCG TCTGGTTCGA TGGGTTCTTC GTCCATGGAG 
GAAGATTACA TGGATCTCTT TGAAATGGAA TCACTTGAGA ATTCAATGAT GCCATCTAAT 
AACATGCCAA AAGTACCCAG CGATCTAGAC TCGTTGTTTG TCGGTCAAAT CAAGAATATA 
TTATCACCTG CCCACCGTTC AGTCGAACAG TCACAATTGT TCGACCAACT GAAAACGCCA 
GAGATGCGTC GTCCACCAGT GCGCCGTTGC CTGAGCATGA CTGACAATTT GCTGCCAGCA 
CCCAAAACGC CAGAGACGCT GCTCAAACAA GTGCAAGAAA TTGCCACCAG CCCCTATGGT 
GTTAAGCTGC TAACAACGGA GCGTAACAAT AGCAACATTA ATTGCTTCAA ACGTCCCGAT 
CCACCATCGA AGACCAACTC ACCGGTACTC ATCAAGCGTT ACCGCATTGA AGCCGAAAAG 
GAGAATCTCG ATGATTCCTT GACAATGTCA CTGTCAATGC TGCCAGCGCC TCAGCCCAAG 
CTCAGCCATC CACCACCATT GCGCAAGAGC ATGTCGATGA ATGATGCCGA AATCATGTCG 
GCGCTCGCAC GCTCCGAGAG CAAGGACGAA CCAGATCTCA TCGGTGATTT CAGCAAACCA 
TATGTACTGC CACTGATGGA TGGACGTCAT CGTGACTTGA AATCAATCTC CTGCGACACA 
ATGGCACGTC TGTTGCGTGG CGATTTCAAT CATGTTGTGG CCAACTATCG CATCATCGAC 
TGTCGTTATC CATACGAATT CGAGGGTGGC CACATACGCG GCGCACAAAA CCTGTACACA 
CAGGAACAGA TACTCGAGGA GTTCCTCACA CAACAAAAGA CTGAAATGGA ACAGCGTAAA 
GCAAGCAATG ATCATCGCCG CAATATCATC ATATTCCACT GCGAATTCTC ATCTGAGCGT 
GGCCCGAAAA TGTCGCGTTT CCTGCGTAGC AACGATCGTG AGCGCAACAC CAATTGCTAT 
CCCGCACTCG ACTACCCCGA GATCTACTTG CTACACAATG GGTACAAAGA ATTCTACGAA 
CATCATTCCG AGTTGTGCGA TCCCACTGCC TATCGGCCCA TGTTGGAGCC AGCATACAAC 
AGCGCCTATC GTCACTTCCG CTCCAAGACA AAGTCGTGGA ACGGTGATGC CGGTTTGGGC 
GGTGCTACCG GGCGTCTAAA GAAATCTCGT TCACGACTAA TGCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011181847.1
CDS	665..2332
Translation	MMWDTIAEETNGEMAMEFASCQMPHNLNSAAASSNGASRQRQSLNILNSSMNDMAVDHQLSIYGDDEHAELNQVENLRSADFDMPQQMGLLSPEGSPCRFQIVQKPRASNGQQRQSFASRLQKLQHNNTGNGSASTPTPPKSLRIFHSLSSGSMGSSSMEEDYMDLFEMESLENSMMPSNNMPKVPSDLDSLFVGQIKNILSPAHRSVEQSQLFDQLKTPEMRRPPVRRCLSMTDNLLPAPKTPETLLKQVQEIATSPYGVKLLTTERNNSNINCFKRPDPPSKTNSPVLIKRYRIEAEKENLDDSLTMSLSMLPAPQPKLSHPPPLRKSMSMNDAEIMSALARSESKDEPDLIGDFSKPYVLPLMDGRHRDLKSISCDTMARLLRGDFNHVVANYRIIDCRYPYEFEGGHIRGAQNLYTQEQILEEFLTQQKTEMEQRKASNDHRRNIIIFHCEFSSERGPKMSRFLRSNDRERNTNCYPALDYPEIYLLHNGYKEFYEHHSELCDPTAYRPMLEPAYNSAYRHFRSKTKSWNGDAGLGGATGRLKKSRSRLML

Target ORF information:

RefSeq Version	XM_011183545.2
Organism	Zeugodacus cucurbitae(melon fly)
Definition	Zeugodacus cucurbitae M-phase inducer phosphatase (LOC105211875), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011183545.2

ORF Insert Sequence:

ATGATGTGGG ACACAATTGC CGAAGAAACC AACGGAGAAA TGGCCATGGA ATTTGCAAGC 
TGTCAGATGC CACACAACTT GAATTCTGCC GCTGCTAGCA GTAATGGAGC CAGCCGTCAA 
CGCCAGTCGT TGAACATCTT GAACAGCTCG ATGAACGACA TGGCAGTCGA TCATCAGTTG 
TCAATCTATG GCGATGACGA ACATGCGGAG CTGAATCAAG TCGAAAACTT GCGTTCGGCC 
GATTTCGATA TGCCACAACA GATGGGTTTG CTGTCACCTG AAGGCTCACC ATGCCGTTTC 
CAAATAGTAC AAAAACCAAG AGCATCGAAT GGTCAACAGC GCCAGTCATT TGCATCACGC 
CTACAGAAGC TACAACATAA CAATACCGGC AATGGCAGTG CTAGCACACC AACACCACCG 
AAGAGTCTGC GTATTTTCCA CAGCTTGTCG TCTGGTTCGA TGGGTTCTTC GTCCATGGAG 
GAAGATTACA TGGATCTCTT TGAAATGGAA TCACTTGAGA ATTCAATGAT GCCATCTAAT 
AACATGCCAA AAGTACCCAG CGATCTAGAC TCGTTGTTTG TCGGTCAAAT CAAGAATATA 
TTATCACCTG CCCACCGTTC AGTCGAACAG TCACAATTGT TCGACCAACT GAAAACGCCA 
GAGATGCGTC GTCCACCAGT GCGCCGTTGC CTGAGCATGA CTGACAATTT GCTGCCAGCA 
CCCAAAACGC CAGAGACGCT GCTCAAACAA GTGCAAGAAA TTGCCACCAG CCCCTATGGT 
GTTAAGCTGC TAACAACGGA GCGTAACAAT AGCAACATTA ATTGCTTCAA ACGTCCCGAT 
CCACCATCGA AGACCAACTC ACCGGTACTC ATCAAGCGTT ACCGCATTGA AGCCGAAAAG 
GAGAATCTCG ATGATTCCTT GACAATGTCA CTGTCAATGC TGCCAGCGCC TCAGCCCAAG 
CTCAGCCATC CACCACCATT GCGCAAGAGC ATGTCGATGA ATGATGCCGA AATCATGTCG 
GCGCTCGCAC GCTCCGAGAG CAAGGACGAA CCAGATCTCA TCGGTGATTT CAGCAAACCA 
TATGTACTGC CACTGATGGA TGGACGTCAT CGTGACTTGA AATCAATCTC CTGCGACACA 
ATGGCACGTC TGTTGCGTGG CGATTTCAAT CATGTTGTGG CCAACTATCG CATCATCGAC 
TGTCGTTATC CATACGAATT CGAGGGTGGC CACATACGCG GCGCACAAAA CCTGTACACA 
CAGGAACAGA TACTCGAGGA GTTCCTCACA CAACAAAAGA CTGAAATGGA ACAGCGTAAA 
GCAAGCAATG ATCATCGCCG CAATATCATC ATATTCCACT GCGAATTCTC ATCTGAGCGT 
GGCCCGAAAA TGTCGCGTTT CCTGCGTAGC AACGATCGTG AGCGCAACAC CAATTGCTAT 
CCCGCACTCG ACTACCCCGA GATCTACTTG CTACACAATG GGTACAAAGA ATTCTACGAA 
CATCATTCCG AGTTGTGCGA TCCCACTGCC TATCGGCCCA TGTTGGAGCC AGCATACAAC 
AGCGCCTATC GTCACTTCCG CTCCAAGACA AAGTCGTGGA ACGGTGATGC CGGTTTGGGC 
GGTGCTACCG GGCGTCTAAA GAAATCTCGT TCACGACTAA TGCTGTAA