LOC105209502 cDNA ORF clone, Zeugodacus cucurbitae(melon fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC105209502
Entrez Gene ID	105209502
Full Name	beta-mannosidase-like
Gene Type	protein-coding
Organism	Zeugodacus cucurbitae(melon fly)

mRNA and Protein(s)

mRNA	Protein	Name
XM_011179918.2	XP_011178220.1	beta-mannosidase
XM_011179918.1	XP_011178220.1	beta-mannosidase-like

Pathways from BioSystems
Gene Ontology

The following LOC105209502 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC105209502 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa195315	XM_011179918.2 Latest version!	Zeugodacus cucurbitae beta-mannosidase (LOC105209502), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
View Old Accession Versions >>
OBa195315	XM_011179918.1	Bactrocera cucurbitae beta-mannosidase-like (LOC105209502), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC105209502 ( XM_011179918.2 ) cDNA ORF clone, Zeugodacus cucurbitae(melon fly) -> XP_011178220.1 Zeugodacus cucurbitae beta-mannosidase (LOC105209502), mRNA.

Price & Availability↑

CloneID	OBa195315
Clone ID Related Accession (Same CDS sequence)	XM_011179918.2 , XM_011179918.1
Accession Version	XM_011179918.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2736bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-27
Organism	Zeugodacus cucurbitae(melon fly)
Product	beta-mannosidase
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011863679.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 28, 2019 this sequence version replaced XM_011179918.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Zeugodacus cucurbitae Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGAATTA TGGAGCAGAA AAGTGCGGTA AACCCTCGCG CTAGTCTAAT TGTGCTGTGC 
GTCAGCGTTT GGTTGTGTAG TTTAAGCGGC TGTGCAGTGG CGGAGTCGGT GTATGTGCAG 
GATTTGAAGG GTCTCTCGTG GACACTAACT AATGGCACTT ACACTAATAC GAAGGTGAAA 
ATACCATCGG GTGTCTACAG CGCTGTAGCG GGTGAAGATG TACTCAAGTC CTACAATGAC 
ATTGACATGC GTTACATTTC TTATGGCAAC TGGACCTATT CACTGGAGTT TGCTGAAGAA 
ATCAAAGACT CGCGTCTAGT CAACCTCACA TTCCATGGTA TTGACACGAT TTCGGAAATT 
TATCTCAATG GTCATTTACT GGGTCACACC GACAACATGT TCGTACGCTA CACCTACGAA 
GTGGGTCACA TTTTACAGGA TGTCAATCAA TTGGTGGTGC GTCTGATCTC GCCGGTACAT 
GCGGCGCGTA CGAGAGCCGA TGAGTTGACG GCGCGCAACA TGTCTGTGCC ACCCGCATGC 
CCACCCGTCA GTTATCACGG TGAATGCCAC ATGAATATGC TGCGTAAAAT GCAGGCGAGC 
TTCTCTTGGG ATTGGGGGCT GGCGGCGCCT TCAATGGGTA TCTGGAAGCC AGTAACGCTG 
GAGTTCTATG AGGTGGCTTT GATACGCGAT GTGGATGTGG CAATTAGTCG TAATGAAACG 
CATTGGAATA TGCACATACG CGTGTTTATC GATAGCGGTG GCAGGCAGGA TTTCTATGCT 
GAGCTGAAAT TTTATGCGAT CGAGCTTATG GAGGAACCCA TTACAATAAG TACTTATTCG 
AAGCAAAAGT TATCATGGCA GAATCCCGTT ATTACCTTCG ATCAAGCGCT ACCCATTGAA 
AACGTGACTC TTTGGTGGCC CAACGGTTAT GGCGATCAAA AATTATATCC ACTACATTTT 
TCCATGAAAG CCTGGCTCAA TCGGAACGGG CTCAATCTGC GAGCGCGTAC TACATCTACG 
AAGAATATAC GAGTAGGTTT TCGTACGATC GAGTTGGTTG AAGACGCGGC CAAAGAAGGT 
GTCGGCAACT ATTTCCTGTT CAAAGTGAAT GGCGTGGAGA TCTTCATGAA GGGCACCAAC 
TACATACCCA TGCATATACT GCCGGAGCTG AGCTACGACG AAAATCAAAT ACGTTATCTG 
CTGACGGCCG TCAAGGAGGC GAATATGAAT ATGATACGCA TTTGGGGTGG CGGCGTCTAC 
GAGAGCAACG AGTTCTATGA TAAAGCCGAC AAATATGGCA TACTCGTGTG GCAGGATATG 
ATGTTCGCAT GCGCTATGTA TCCCGCTACA GATGATTTTC TCGCTTCGGT GCGCACAGAA 
GTCATACAAA ATGCTAAACG CATCGCTTAC CATCCGAGTA TTGCGATTAT AGCGACGAAT 
AATGAAAATG AGGCGGCGCT CGTGGATAAC TGGTATGGCA CGGCCGGTGA TCGGTCGCGT 
TTCGAGGCGG AATATCGTGA GCTGTATATT GGCACCGTTT TTCATGAGTT GAAGATCATA 
CAGGATAGCT CACGCCCGAA ACCACTGATA TCCTCGCCCT CGAATGCAAA GGCGAGTGAA 
AAGGATAATT ATATTTCGAA TAATCCGGGC GATGAGAATT ATGGCGATAT ACACTTCTAT 
ACGAACTTTG AGGACGCGTG GAATCCAAAC ACCTATCGAC GTCCGCGCTT CGCTTCCGAG 
TATGGTTTTC AGAGTTTGCC GAAACTTACT TCCTGGCAGT TGACTATGAA CCCTAAAGAT 
GATCTGCTCA ATTTAATACA TCACCGTCAG CATCATCCCT CTAATATGAC AATGATAATT 
GCATTGATAA GGCGCCATTT GCCGTTGCCA CTACCTGAGG ATGACAATTA TGTCGATGCC 
TTGATCTATT TCAGTCAAAT TTCGCAGGCG ATGGTGACAA AAGTAGAGAC CGAAGTCTAT 
CGCAGTTTGA GAGACACAGA ACATCATACA ATGGGCGCAC TCTACTGGCA GTTGAATGAT 
GTTTGGGTGG CGCCATCTTG GTCCAGCATT GATTTCTATG GAAATTTCAA GTTACTACAC 
TATTGGGCGA AATACTTCAT GGCACCCATA CATATTGTGG CACTCTACGA CTCCGATCAG 
GAAGCAGTGA ATGTGTCCTT AATTTGCGAT CAGTTGCAAG TGGACCACAA AGGGCTTCAC 
GTATCTATGA ATATCTACAA ATGGTCGGAC TTGTTTCCGA AAAAACAAAT TGTTTGGCCG 
GTCGCTTTGA AACCCAATGG TGTGCAGTAC GACAAGATAA TACCGATTGA TACCATTTTC 
GAAGGGGCCT TCACTAAAAC CAACTCTTTT GTTGAATTCG TTTTGAGTGA CGAATCAACT 
GTACTTGCGC GTAATTATTA CTTCCCCAGC GCGATTAAGA ATGCTGTGGG TATGGAGGAT 
CCCAAAGTAA CGTTGAGCGT TTTCTTCACC TACTGCTACC GTGTGAACAA TCAATTCGCC 
AATATTATCA GCCTGAATGT TAACGTTGCA AAGCCTGCCG TATTCCTCTA TCTCGAACTA 
CTTAAGGCCA ATATTACCAG ATATAAATTA TCGAATAACG GCTTTATGAT GTTGGTGCCG 
GCGAAGATGG TGCATTTGGA GTTCAATAGT GATGAATGTA TGGATGTCAA AGTGAGCGAT 
TTTAAGGTTA CAACCGTAAA CCAATATATG ATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011178220.1
CDS	274..3009
Translation	MRIMEQKSAVNPRASLIVLCVSVWLCSLSGCAVAESVYVQDLKGLSWTLTNGTYTNTKVKIPSGVYSAVAGEDVLKSYNDIDMRYISYGNWTYSLEFAEEIKDSRLVNLTFHGIDTISEIYLNGHLLGHTDNMFVRYTYEVGHILQDVNQLVVRLISPVHAARTRADELTARNMSVPPACPPVSYHGECHMNMLRKMQASFSWDWGLAAPSMGIWKPVTLEFYEVALIRDVDVAISRNETHWNMHIRVFIDSGGRQDFYAELKFYAIELMEEPITISTYSKQKLSWQNPVITFDQALPIENVTLWWPNGYGDQKLYPLHFSMKAWLNRNGLNLRARTTSTKNIRVGFRTIELVEDAAKEGVGNYFLFKVNGVEIFMKGTNYIPMHILPELSYDENQIRYLLTAVKEANMNMIRIWGGGVYESNEFYDKADKYGILVWQDMMFACAMYPATDDFLASVRTEVIQNAKRIAYHPSIAIIATNNENEAALVDNWYGTAGDRSRFEAEYRELYIGTVFHELKIIQDSSRPKPLISSPSNAKASEKDNYISNNPGDENYGDIHFYTNFEDAWNPNTYRRPRFASEYGFQSLPKLTSWQLTMNPKDDLLNLIHHRQHHPSNMTMIIALIRRHLPLPLPEDDNYVDALIYFSQISQAMVTKVETEVYRSLRDTEHHTMGALYWQLNDVWVAPSWSSIDFYGNFKLLHYWAKYFMAPIHIVALYDSDQEAVNVSLICDQLQVDHKGLHVSMNIYKWSDLFPKKQIVWPVALKPNGVQYDKIIPIDTIFEGAFTKTNSFVEFVLSDESTVLARNYYFPSAIKNAVGMEDPKVTLSVFFTYCYRVNNQFANIISLNVNVAKPAVFLYLELLKANITRYKLSNNGFMMLVPAKMVHLEFNSDECMDVKVSDFKVTTVNQYMI

Target ORF information:

RefSeq Version	XM_011179918.2
Organism	Zeugodacus cucurbitae(melon fly)
Definition	Zeugodacus cucurbitae beta-mannosidase (LOC105209502), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011179918.2

ORF Insert Sequence:

ATGCGAATTA TGGAGCAGAA AAGTGCGGTA AACCCTCGCG CTAGTCTAAT TGTGCTGTGC 
GTCAGCGTTT GGTTGTGTAG TTTAAGCGGC TGTGCAGTGG CGGAGTCGGT GTATGTGCAG 
GATTTGAAGG GTCTCTCGTG GACACTAACT AATGGCACTT ACACTAATAC GAAGGTGAAA 
ATACCATCGG GTGTCTACAG CGCTGTAGCG GGTGAAGATG TACTCAAGTC CTACAATGAC 
ATTGACATGC GTTACATTTC TTATGGCAAC TGGACCTATT CACTGGAGTT TGCTGAAGAA 
ATCAAAGACT CGCGTCTAGT CAACCTCACA TTCCATGGTA TTGACACGAT TTCGGAAATT 
TATCTCAATG GTCATTTACT GGGTCACACC GACAACATGT TCGTACGCTA CACCTACGAA 
GTGGGTCACA TTTTACAGGA TGTCAATCAA TTGGTGGTGC GTCTGATCTC GCCGGTACAT 
GCGGCGCGTA CGAGAGCCGA TGAGTTGACG GCGCGCAACA TGTCTGTGCC ACCCGCATGC 
CCACCCGTCA GTTATCACGG TGAATGCCAC ATGAATATGC TGCGTAAAAT GCAGGCGAGC 
TTCTCTTGGG ATTGGGGGCT GGCGGCGCCT TCAATGGGTA TCTGGAAGCC AGTAACGCTG 
GAGTTCTATG AGGTGGCTTT GATACGCGAT GTGGATGTGG CAATTAGTCG TAATGAAACG 
CATTGGAATA TGCACATACG CGTGTTTATC GATAGCGGTG GCAGGCAGGA TTTCTATGCT 
GAGCTGAAAT TTTATGCGAT CGAGCTTATG GAGGAACCCA TTACAATAAG TACTTATTCG 
AAGCAAAAGT TATCATGGCA GAATCCCGTT ATTACCTTCG ATCAAGCGCT ACCCATTGAA 
AACGTGACTC TTTGGTGGCC CAACGGTTAT GGCGATCAAA AATTATATCC ACTACATTTT 
TCCATGAAAG CCTGGCTCAA TCGGAACGGG CTCAATCTGC GAGCGCGTAC TACATCTACG 
AAGAATATAC GAGTAGGTTT TCGTACGATC GAGTTGGTTG AAGACGCGGC CAAAGAAGGT 
GTCGGCAACT ATTTCCTGTT CAAAGTGAAT GGCGTGGAGA TCTTCATGAA GGGCACCAAC 
TACATACCCA TGCATATACT GCCGGAGCTG AGCTACGACG AAAATCAAAT ACGTTATCTG 
CTGACGGCCG TCAAGGAGGC GAATATGAAT ATGATACGCA TTTGGGGTGG CGGCGTCTAC 
GAGAGCAACG AGTTCTATGA TAAAGCCGAC AAATATGGCA TACTCGTGTG GCAGGATATG 
ATGTTCGCAT GCGCTATGTA TCCCGCTACA GATGATTTTC TCGCTTCGGT GCGCACAGAA 
GTCATACAAA ATGCTAAACG CATCGCTTAC CATCCGAGTA TTGCGATTAT AGCGACGAAT 
AATGAAAATG AGGCGGCGCT CGTGGATAAC TGGTATGGCA CGGCCGGTGA TCGGTCGCGT 
TTCGAGGCGG AATATCGTGA GCTGTATATT GGCACCGTTT TTCATGAGTT GAAGATCATA 
CAGGATAGCT CACGCCCGAA ACCACTGATA TCCTCGCCCT CGAATGCAAA GGCGAGTGAA 
AAGGATAATT ATATTTCGAA TAATCCGGGC GATGAGAATT ATGGCGATAT ACACTTCTAT 
ACGAACTTTG AGGACGCGTG GAATCCAAAC ACCTATCGAC GTCCGCGCTT CGCTTCCGAG 
TATGGTTTTC AGAGTTTGCC GAAACTTACT TCCTGGCAGT TGACTATGAA CCCTAAAGAT 
GATCTGCTCA ATTTAATACA TCACCGTCAG CATCATCCCT CTAATATGAC AATGATAATT 
GCATTGATAA GGCGCCATTT GCCGTTGCCA CTACCTGAGG ATGACAATTA TGTCGATGCC 
TTGATCTATT TCAGTCAAAT TTCGCAGGCG ATGGTGACAA AAGTAGAGAC CGAAGTCTAT 
CGCAGTTTGA GAGACACAGA ACATCATACA ATGGGCGCAC TCTACTGGCA GTTGAATGAT 
GTTTGGGTGG CGCCATCTTG GTCCAGCATT GATTTCTATG GAAATTTCAA GTTACTACAC 
TATTGGGCGA AATACTTCAT GGCACCCATA CATATTGTGG CACTCTACGA CTCCGATCAG 
GAAGCAGTGA ATGTGTCCTT AATTTGCGAT CAGTTGCAAG TGGACCACAA AGGGCTTCAC 
GTATCTATGA ATATCTACAA ATGGTCGGAC TTGTTTCCGA AAAAACAAAT TGTTTGGCCG 
GTCGCTTTGA AACCCAATGG TGTGCAGTAC GACAAGATAA TACCGATTGA TACCATTTTC 
GAAGGGGCCT TCACTAAAAC CAACTCTTTT GTTGAATTCG TTTTGAGTGA CGAATCAACT 
GTACTTGCGC GTAATTATTA CTTCCCCAGC GCGATTAAGA ATGCTGTGGG TATGGAGGAT 
CCCAAAGTAA CGTTGAGCGT TTTCTTCACC TACTGCTACC GTGTGAACAA TCAATTCGCC 
AATATTATCA GCCTGAATGT TAACGTTGCA AAGCCTGCCG TATTCCTCTA TCTCGAACTA 
CTTAAGGCCA ATATTACCAG ATATAAATTA TCGAATAACG GCTTTATGAT GTTGGTGCCG 
GCGAAGATGG TGCATTTGGA GTTCAATAGT GATGAATGTA TGGATGTCAA AGTGAGCGAT 
TTTAAGGTTA CAACCGTAAA CCAATATATG ATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC105209502 ( XM_011179918.1 ) cDNA ORF clone, Zeugodacus cucurbitae(melon fly) -> XP_011178220.1 Bactrocera cucurbitae beta-mannosidase-like (LOC105209502), mRNA.

Price & Availability↑

CloneID	OBa195315
Clone ID Related Accession (Same CDS sequence)	XM_011179918.2 , XM_011179918.1
Accession Version	XM_011179918.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2736bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-01-28
Organism	Zeugodacus cucurbitae(melon fly)
Product	beta-mannosidase-like
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011863679.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bactrocera cucurbitae Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGAATTA TGGAGCAGAA AAGTGCGGTA AACCCTCGCG CTAGTCTAAT TGTGCTGTGC 
GTCAGCGTTT GGTTGTGTAG TTTAAGCGGC TGTGCAGTGG CGGAGTCGGT GTATGTGCAG 
GATTTGAAGG GTCTCTCGTG GACACTAACT AATGGCACTT ACACTAATAC GAAGGTGAAA 
ATACCATCGG GTGTCTACAG CGCTGTAGCG GGTGAAGATG TACTCAAGTC CTACAATGAC 
ATTGACATGC GTTACATTTC TTATGGCAAC TGGACCTATT CACTGGAGTT TGCTGAAGAA 
ATCAAAGACT CGCGTCTAGT CAACCTCACA TTCCATGGTA TTGACACGAT TTCGGAAATT 
TATCTCAATG GTCATTTACT GGGTCACACC GACAACATGT TCGTACGCTA CACCTACGAA 
GTGGGTCACA TTTTACAGGA TGTCAATCAA TTGGTGGTGC GTCTGATCTC GCCGGTACAT 
GCGGCGCGTA CGAGAGCCGA TGAGTTGACG GCGCGCAACA TGTCTGTGCC ACCCGCATGC 
CCACCCGTCA GTTATCACGG TGAATGCCAC ATGAATATGC TGCGTAAAAT GCAGGCGAGC 
TTCTCTTGGG ATTGGGGGCT GGCGGCGCCT TCAATGGGTA TCTGGAAGCC AGTAACGCTG 
GAGTTCTATG AGGTGGCTTT GATACGCGAT GTGGATGTGG CAATTAGTCG TAATGAAACG 
CATTGGAATA TGCACATACG CGTGTTTATC GATAGCGGTG GCAGGCAGGA TTTCTATGCT 
GAGCTGAAAT TTTATGCGAT CGAGCTTATG GAGGAACCCA TTACAATAAG TACTTATTCG 
AAGCAAAAGT TATCATGGCA GAATCCCGTT ATTACCTTCG ATCAAGCGCT ACCCATTGAA 
AACGTGACTC TTTGGTGGCC CAACGGTTAT GGCGATCAAA AATTATATCC ACTACATTTT 
TCCATGAAAG CCTGGCTCAA TCGGAACGGG CTCAATCTGC GAGCGCGTAC TACATCTACG 
AAGAATATAC GAGTAGGTTT TCGTACGATC GAGTTGGTTG AAGACGCGGC CAAAGAAGGT 
GTCGGCAACT ATTTCCTGTT CAAAGTGAAT GGCGTGGAGA TCTTCATGAA GGGCACCAAC 
TACATACCCA TGCATATACT GCCGGAGCTG AGCTACGACG AAAATCAAAT ACGTTATCTG 
CTGACGGCCG TCAAGGAGGC GAATATGAAT ATGATACGCA TTTGGGGTGG CGGCGTCTAC 
GAGAGCAACG AGTTCTATGA TAAAGCCGAC AAATATGGCA TACTCGTGTG GCAGGATATG 
ATGTTCGCAT GCGCTATGTA TCCCGCTACA GATGATTTTC TCGCTTCGGT GCGCACAGAA 
GTCATACAAA ATGCTAAACG CATCGCTTAC CATCCGAGTA TTGCGATTAT AGCGACGAAT 
AATGAAAATG AGGCGGCGCT CGTGGATAAC TGGTATGGCA CGGCCGGTGA TCGGTCGCGT 
TTCGAGGCGG AATATCGTGA GCTGTATATT GGCACCGTTT TTCATGAGTT GAAGATCATA 
CAGGATAGCT CACGCCCGAA ACCACTGATA TCCTCGCCCT CGAATGCAAA GGCGAGTGAA 
AAGGATAATT ATATTTCGAA TAATCCGGGC GATGAGAATT ATGGCGATAT ACACTTCTAT 
ACGAACTTTG AGGACGCGTG GAATCCAAAC ACCTATCGAC GTCCGCGCTT CGCTTCCGAG 
TATGGTTTTC AGAGTTTGCC GAAACTTACT TCCTGGCAGT TGACTATGAA CCCTAAAGAT 
GATCTGCTCA ATTTAATACA TCACCGTCAG CATCATCCCT CTAATATGAC AATGATAATT 
GCATTGATAA GGCGCCATTT GCCGTTGCCA CTACCTGAGG ATGACAATTA TGTCGATGCC 
TTGATCTATT TCAGTCAAAT TTCGCAGGCG ATGGTGACAA AAGTAGAGAC CGAAGTCTAT 
CGCAGTTTGA GAGACACAGA ACATCATACA ATGGGCGCAC TCTACTGGCA GTTGAATGAT 
GTTTGGGTGG CGCCATCTTG GTCCAGCATT GATTTCTATG GAAATTTCAA GTTACTACAC 
TATTGGGCGA AATACTTCAT GGCACCCATA CATATTGTGG CACTCTACGA CTCCGATCAG 
GAAGCAGTGA ATGTGTCCTT AATTTGCGAT CAGTTGCAAG TGGACCACAA AGGGCTTCAC 
GTATCTATGA ATATCTACAA ATGGTCGGAC TTGTTTCCGA AAAAACAAAT TGTTTGGCCG 
GTCGCTTTGA AACCCAATGG TGTGCAGTAC GACAAGATAA TACCGATTGA TACCATTTTC 
GAAGGGGCCT TCACTAAAAC CAACTCTTTT GTTGAATTCG TTTTGAGTGA CGAATCAACT 
GTACTTGCGC GTAATTATTA CTTCCCCAGC GCGATTAAGA ATGCTGTGGG TATGGAGGAT 
CCCAAAGTAA CGTTGAGCGT TTTCTTCACC TACTGCTACC GTGTGAACAA TCAATTCGCC 
AATATTATCA GCCTGAATGT TAACGTTGCA AAGCCTGCCG TATTCCTCTA TCTCGAACTA 
CTTAAGGCCA ATATTACCAG ATATAAATTA TCGAATAACG GCTTTATGAT GTTGGTGCCG 
GCGAAGATGG TGCATTTGGA GTTCAATAGT GATGAATGTA TGGATGTCAA AGTGAGCGAT 
TTTAAGGTTA CAACCGTAAA CCAATATATG ATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011178220.1
CDS	272..3007
Translation	MRIMEQKSAVNPRASLIVLCVSVWLCSLSGCAVAESVYVQDLKGLSWTLTNGTYTNTKVKIPSGVYSAVAGEDVLKSYNDIDMRYISYGNWTYSLEFAEEIKDSRLVNLTFHGIDTISEIYLNGHLLGHTDNMFVRYTYEVGHILQDVNQLVVRLISPVHAARTRADELTARNMSVPPACPPVSYHGECHMNMLRKMQASFSWDWGLAAPSMGIWKPVTLEFYEVALIRDVDVAISRNETHWNMHIRVFIDSGGRQDFYAELKFYAIELMEEPITISTYSKQKLSWQNPVITFDQALPIENVTLWWPNGYGDQKLYPLHFSMKAWLNRNGLNLRARTTSTKNIRVGFRTIELVEDAAKEGVGNYFLFKVNGVEIFMKGTNYIPMHILPELSYDENQIRYLLTAVKEANMNMIRIWGGGVYESNEFYDKADKYGILVWQDMMFACAMYPATDDFLASVRTEVIQNAKRIAYHPSIAIIATNNENEAALVDNWYGTAGDRSRFEAEYRELYIGTVFHELKIIQDSSRPKPLISSPSNAKASEKDNYISNNPGDENYGDIHFYTNFEDAWNPNTYRRPRFASEYGFQSLPKLTSWQLTMNPKDDLLNLIHHRQHHPSNMTMIIALIRRHLPLPLPEDDNYVDALIYFSQISQAMVTKVETEVYRSLRDTEHHTMGALYWQLNDVWVAPSWSSIDFYGNFKLLHYWAKYFMAPIHIVALYDSDQEAVNVSLICDQLQVDHKGLHVSMNIYKWSDLFPKKQIVWPVALKPNGVQYDKIIPIDTIFEGAFTKTNSFVEFVLSDESTVLARNYYFPSAIKNAVGMEDPKVTLSVFFTYCYRVNNQFANIISLNVNVAKPAVFLYLELLKANITRYKLSNNGFMMLVPAKMVHLEFNSDECMDVKVSDFKVTTVNQYMI

Target ORF information:

RefSeq Version	XM_011179918.1
Organism	Zeugodacus cucurbitae(melon fly)
Definition	Bactrocera cucurbitae beta-mannosidase-like (LOC105209502), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011179918.1

ORF Insert Sequence:

ATGCGAATTA TGGAGCAGAA AAGTGCGGTA AACCCTCGCG CTAGTCTAAT TGTGCTGTGC 
GTCAGCGTTT GGTTGTGTAG TTTAAGCGGC TGTGCAGTGG CGGAGTCGGT GTATGTGCAG 
GATTTGAAGG GTCTCTCGTG GACACTAACT AATGGCACTT ACACTAATAC GAAGGTGAAA 
ATACCATCGG GTGTCTACAG CGCTGTAGCG GGTGAAGATG TACTCAAGTC CTACAATGAC 
ATTGACATGC GTTACATTTC TTATGGCAAC TGGACCTATT CACTGGAGTT TGCTGAAGAA 
ATCAAAGACT CGCGTCTAGT CAACCTCACA TTCCATGGTA TTGACACGAT TTCGGAAATT 
TATCTCAATG GTCATTTACT GGGTCACACC GACAACATGT TCGTACGCTA CACCTACGAA 
GTGGGTCACA TTTTACAGGA TGTCAATCAA TTGGTGGTGC GTCTGATCTC GCCGGTACAT 
GCGGCGCGTA CGAGAGCCGA TGAGTTGACG GCGCGCAACA TGTCTGTGCC ACCCGCATGC 
CCACCCGTCA GTTATCACGG TGAATGCCAC ATGAATATGC TGCGTAAAAT GCAGGCGAGC 
TTCTCTTGGG ATTGGGGGCT GGCGGCGCCT TCAATGGGTA TCTGGAAGCC AGTAACGCTG 
GAGTTCTATG AGGTGGCTTT GATACGCGAT GTGGATGTGG CAATTAGTCG TAATGAAACG 
CATTGGAATA TGCACATACG CGTGTTTATC GATAGCGGTG GCAGGCAGGA TTTCTATGCT 
GAGCTGAAAT TTTATGCGAT CGAGCTTATG GAGGAACCCA TTACAATAAG TACTTATTCG 
AAGCAAAAGT TATCATGGCA GAATCCCGTT ATTACCTTCG ATCAAGCGCT ACCCATTGAA 
AACGTGACTC TTTGGTGGCC CAACGGTTAT GGCGATCAAA AATTATATCC ACTACATTTT 
TCCATGAAAG CCTGGCTCAA TCGGAACGGG CTCAATCTGC GAGCGCGTAC TACATCTACG 
AAGAATATAC GAGTAGGTTT TCGTACGATC GAGTTGGTTG AAGACGCGGC CAAAGAAGGT 
GTCGGCAACT ATTTCCTGTT CAAAGTGAAT GGCGTGGAGA TCTTCATGAA GGGCACCAAC 
TACATACCCA TGCATATACT GCCGGAGCTG AGCTACGACG AAAATCAAAT ACGTTATCTG 
CTGACGGCCG TCAAGGAGGC GAATATGAAT ATGATACGCA TTTGGGGTGG CGGCGTCTAC 
GAGAGCAACG AGTTCTATGA TAAAGCCGAC AAATATGGCA TACTCGTGTG GCAGGATATG 
ATGTTCGCAT GCGCTATGTA TCCCGCTACA GATGATTTTC TCGCTTCGGT GCGCACAGAA 
GTCATACAAA ATGCTAAACG CATCGCTTAC CATCCGAGTA TTGCGATTAT AGCGACGAAT 
AATGAAAATG AGGCGGCGCT CGTGGATAAC TGGTATGGCA CGGCCGGTGA TCGGTCGCGT 
TTCGAGGCGG AATATCGTGA GCTGTATATT GGCACCGTTT TTCATGAGTT GAAGATCATA 
CAGGATAGCT CACGCCCGAA ACCACTGATA TCCTCGCCCT CGAATGCAAA GGCGAGTGAA 
AAGGATAATT ATATTTCGAA TAATCCGGGC GATGAGAATT ATGGCGATAT ACACTTCTAT 
ACGAACTTTG AGGACGCGTG GAATCCAAAC ACCTATCGAC GTCCGCGCTT CGCTTCCGAG 
TATGGTTTTC AGAGTTTGCC GAAACTTACT TCCTGGCAGT TGACTATGAA CCCTAAAGAT 
GATCTGCTCA ATTTAATACA TCACCGTCAG CATCATCCCT CTAATATGAC AATGATAATT 
GCATTGATAA GGCGCCATTT GCCGTTGCCA CTACCTGAGG ATGACAATTA TGTCGATGCC 
TTGATCTATT TCAGTCAAAT TTCGCAGGCG ATGGTGACAA AAGTAGAGAC CGAAGTCTAT 
CGCAGTTTGA GAGACACAGA ACATCATACA ATGGGCGCAC TCTACTGGCA GTTGAATGAT 
GTTTGGGTGG CGCCATCTTG GTCCAGCATT GATTTCTATG GAAATTTCAA GTTACTACAC 
TATTGGGCGA AATACTTCAT GGCACCCATA CATATTGTGG CACTCTACGA CTCCGATCAG 
GAAGCAGTGA ATGTGTCCTT AATTTGCGAT CAGTTGCAAG TGGACCACAA AGGGCTTCAC 
GTATCTATGA ATATCTACAA ATGGTCGGAC TTGTTTCCGA AAAAACAAAT TGTTTGGCCG 
GTCGCTTTGA AACCCAATGG TGTGCAGTAC GACAAGATAA TACCGATTGA TACCATTTTC 
GAAGGGGCCT TCACTAAAAC CAACTCTTTT GTTGAATTCG TTTTGAGTGA CGAATCAACT 
GTACTTGCGC GTAATTATTA CTTCCCCAGC GCGATTAAGA ATGCTGTGGG TATGGAGGAT 
CCCAAAGTAA CGTTGAGCGT TTTCTTCACC TACTGCTACC GTGTGAACAA TCAATTCGCC 
AATATTATCA GCCTGAATGT TAACGTTGCA AAGCCTGCCG TATTCCTCTA TCTCGAACTA 
CTTAAGGCCA ATATTACCAG ATATAAATTA TCGAATAACG GCTTTATGAT GTTGGTGCCG 
GCGAAGATGG TGCATTTGGA GTTCAATAGT GATGAATGTA TGGATGTCAA AGTGAGCGAT 
TTTAAGGTTA CAACCGTAAA CCAATATATG ATATGA