topors cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	topors
Entrez Gene ID	734040
Full Name	topoisomerase I binding, arginine/serine-rich, E3 ubiquitin protein ligase
General protein information	Preferred Names topoisomerase I binding, arginine/serine-rich, E3 ubiquitin protein ligase Names E3 ubiquitin-protein ligase Topors
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_002940264.5	XP_002940310.1	E3 ubiquitin-protein ligase Topors
XM_002940264.4	XP_002940310.1	E3 ubiquitin-protein ligase Topors

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	TOPORS	XP_003639434.1
Bos taurus (cattle)	TOPORS	NP_001179507.2
Mus musculus (house mouse)	Topors	NP_598858.2
Homo sapiens (human)	TOPORS	NP_005793.2
Pan troglodytes (chimpanzee)	TOPORS	XP_003951457.1
	TOPORS	XP_001092273.2
Gallus gallus (chicken)	TOPORS	XP_424920.4
Rattus norvegicus (Norway rat)	Topors	NP_001102128.1
Xenopus tropicalis (tropical clawed frog)	topors	XP_002940310.1

Gene Ontology

The following topors gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the topors cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa04122	XM_002940264.5 Latest version!	Xenopus tropicalis topoisomerase I binding, arginine/serine-rich, E3 ubiquitin protein ligase (topors), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
View Old Accession Versions >>
OXa04122	XM_002940264.4	Xenopus tropicalis topoisomerase I binding, arginine/serine-rich, E3 ubiquitin protein ligase (topors), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

topors ( XM_002940264.5 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002940310.1 Xenopus tropicalis topoisomerase I binding, arginine/serine-rich, E3 ubiquitin protein ligase (topors), mRNA.

Price & Availability↑

CloneID	OXa04122
Clone ID Related Accession (Same CDS sequence)	XM_002940264.5 , XM_002940264.4
Accession Version	XM_002940264.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3057bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	E3 ubiquitin-protein ligase Topors
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002940264.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGGAGA AGAGAACCAA GCGGCGGAGG CCGGGAGCGA GGCAAGAAGC GAATGACACG 
ATGATGGCAT CATCCTCTAA CAATTATTCA CTAGATGGCA GCTTTTCACC CCAGGCTGGC 
ACAAGCAAAC TACAAAACCA TGGTACTGCA ACAGATGCTT CCCCAGACTC AAAGTGTCCA 
ATATGTTTGG ACAGGTTTGA TAATGTGTCA CATCTTGATC GATGTCTGCA CAGATTTTGT 
TTCCGTTGCA TTCAAGAATG GGCAAAAAAC AAAGCTGAAT GCCCTCTATG CAAACAGCCC 
TTTTACTCCA TTTTCCATAG TGTCAAAGCA GAGGATGATT TTAAAGAATA TGTCTTGAGG 
CCCACAATGA ATGGTTCATT TGCCAGTCCT GATGGACATA GATTTCGTTA TCGCACAACT 
ATGAGTAGAG ACAATCATGT AGCCATTCGG ACATTAAGGT CTTCAAGCAC TCAAAGGACA 
TTTTCACCTC CTGACAATGG AATTTTATTT GAAGGGTTTT CAAATCAGAA TTTACATCAA 
AGAGGTGAAG AAATCCACCA GATGATAAGA AGGCTAGCCT CCAGACGACA AGCCAGTGCA 
GAGGGTCGTT CTATGAGGCA AATTCAAGAG CAAGAACTCA TTAACTTCAG AAGAGCTTTG 
TACCGGTCTG GAATACGTGT GAGAAACATT CAGGATGGCG GACGTTATAG AGATATTTCT 
GCTGAGTTCT TCCGGCGTAA CCCTGCTTGT CTTCATCGGC TAGTACCATG GTTGAAAAGA 
GAGTTGACTG TTCTCTTTGG TAGCCATGGT TCCCTTGTTA ACATTGTACA GCATATTATT 
ATGAGTAATG TCACACGATA TGATATGGAA AGTCAAGCAT TTGTTGAGGA TCTTCGTCCT 
TTTTTGCTTC ACCGCACAGA CCATTTCATA CATGAGTTTG TTAACTTTGC CCGATGCCCT 
TACAATATTG AAGCATATGA TCAGCATGCA AACTATGATT GTCCTGCTCC TTCATATGAG 
GAAGGAAGCC GTTCAGAGTC ATCTGTCATC ACAATATCGC CAGATGAAGC AGATACCAGA 
GATCCAATTA TTCCTTCTGC TGGTCCTGGT GTTGGCCAGA CAGCTTGGGA TGATGAGACC 
CCTGGTCCCT CTTATTCCAC ACTTGAGCAA GCCTCAGTTG CTGTATCCAC TACACTAGAT 
ATCTCAGAAA GCTCAGACGA TGAGCCACCC AGCATAAGAC AGACTTCACA AGAAGTTTCC 
AACAGTGAAC CCACAGAAAA CCTGCAGGAT CAGTCCTTTC CAACTGATGA CTGTGTTATA 
GTAGGGTATG TTAAACCACT GGCAGAAAGA ACTCCAGAAT TAGTTGAATT GTCATCCGAC 
TCCGAGAGCT CATTGAGTGA GGTTAAGACA GAAGATGCCA AAAAGCCTAC GATTAAACCA 
TTTTTCCTAA GTGACAGTAG TGAATTGAGC AGGTCATCAT CATCTTTGTA CACACCTGCA 
AAAGAAAAAC GTTCACGCAA AACCAAGGGA AAAACCACAT CTAGCAAACA TTCCCATTCC 
AGGAAAAAAG AGAAGGGAAA ATGTCGACTT GATTTAACTT CTAGAAAGGA TAAGGGGGAT 
TTGAACAGTA ATTATGCTTT GTCCAGATGG AGGGATAGGT CAAGGAGTTC TGATTGTTAT 
TCTCGTTCTT CTCGCAATAA AAGCCACAAC AGTCGAAGGA GGCATTGCAG CAAAAATAAT 
TTGAAACCCA GGAGTCCTGA AAAGAGCAGG CATAAAAGTA GGAAAGGAAG GAAGAAATCT 
AGTAGTAGGG ATCGAAGTCT GTCTTGGAGG AGTAGGACTG TGTCACTAAC TAGTGAAAGC 
TCCAGAGAGA GATGCGAGTC AAGCTCTAGA AGCCGAAATC GAAGTAGGGG TAGGTCACGA 
AGTCATGATA GTGACAATGA TTTTTCTTCC AACAACTATC GCAGTTCTTA TCAATGGGAA 
TATACCTATT ACAGTCGAAA TCGGGATAGA GATGGCTATG AACAGTCCTA CAGGAGACGG 
ACTTGTGGTC GAGGACGTTA TTCAAGACCC TCTGCTAGCC CAGAATATAA AATTCAGTCT 
TACTCTGAAA AGAAAGATAC AAGAAAGGAG AGGGGATACA TTGCTAGTAA ACAATATCGC 
CGTGAGCGAT ACAGGTCAAG GAGTCGCTCA AGCAGTCAGA TGAATACATC AGGGACTGAG 
CATACCAGAT CTGACAAACC AAGTGGAAAA AGAAAATACA AAACACACCA TTTGGAAAAG 
CAGGAAAGGG AAGGGAACAA TCGAGAAGTT CCTTCTGCCA AAGAAAAAGA GGGAGGTCTG 
CAAGCAGGCA AGACTTCTCT TGGCAATCAT ACTGGTAGTC TCAAAAATGA TTTAATGGGT 
TCATCCTCTG AACCTAAGCA GAAGTTAAGA AAAAAGGCAA GAAGCCCAAG TGTGGAAATA 
GTATATGAGG GCAAAGCAGT TGAAGGGGCA AAGCACCACA AAAAGAAGAA GAAAAAGAAG 
CACAAGAAAA AACGACGCAG GGAACACACT AATTCTCCAG CTGCTTCTCC CATCATTATC 
ACCATTGACA GTGACAGTGA CACCCCAATG GTGGAGGATC TCCCATGCAA TGACCACAGC 
TCCCTGGTAC CTGAAAACAA AACTAGTATT GACTCAACTA ACTTCATGCA CAGCCAGTCG 
CCACCTACCT CGACCACAGT AAGTACCACA GAAGTACTAA GTGGAGAAAG GGCAGACTCT 
GATTCCAAAG CCTCTGATTT ATGTTCAAGT AACAGGCACT TAGATGCTGC CACTGGAATA 
CTGGATGGTT TACATTTTGA TGATAGCTCT GATGAACAGA ATCTGCTCCC TGTACAAAGT 
CCTCCTAACA TACCGGATCT CTGTGAAGTA GAACCTTTAG CAGCTGAGAG TTCCTTGCCA 
GAGTCTGATC CTATACTGGA CAATGTACCA CCTACATCAA CTTCAGAAAT ATCTGAACTT 
CATAAAAGCT TTGCTGAGAG TATATTTGAC TTTGATCTCT TAAACAATGT AACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002940310.1
CDS	135..3191
Translation	MREKRTKRRRPGARQEANDTMMASSSNNYSLDGSFSPQAGTSKLQNHGTATDASPDSKCPICLDRFDNVSHLDRCLHRFCFRCIQEWAKNKAECPLCKQPFYSIFHSVKAEDDFKEYVLRPTMNGSFASPDGHRFRYRTTMSRDNHVAIRTLRSSSTQRTFSPPDNGILFEGFSNQNLHQRGEEIHQMIRRLASRRQASAEGRSMRQIQEQELINFRRALYRSGIRVRNIQDGGRYRDISAEFFRRNPACLHRLVPWLKRELTVLFGSHGSLVNIVQHIIMSNVTRYDMESQAFVEDLRPFLLHRTDHFIHEFVNFARCPYNIEAYDQHANYDCPAPSYEEGSRSESSVITISPDEADTRDPIIPSAGPGVGQTAWDDETPGPSYSTLEQASVAVSTTLDISESSDDEPPSIRQTSQEVSNSEPTENLQDQSFPTDDCVIVGYVKPLAERTPELVELSSDSESSLSEVKTEDAKKPTIKPFFLSDSSELSRSSSSLYTPAKEKRSRKTKGKTTSSKHSHSRKKEKGKCRLDLTSRKDKGDLNSNYALSRWRDRSRSSDCYSRSSRNKSHNSRRRHCSKNNLKPRSPEKSRHKSRKGRKKSSSRDRSLSWRSRTVSLTSESSRERCESSSRSRNRSRGRSRSHDSDNDFSSNNYRSSYQWEYTYYSRNRDRDGYEQSYRRRTCGRGRYSRPSASPEYKIQSYSEKKDTRKERGYIASKQYRRERYRSRSRSSSQMNTSGTEHTRSDKPSGKRKYKTHHLEKQEREGNNREVPSAKEKEGGLQAGKTSLGNHTGSLKNDLMGSSSEPKQKLRKKARSPSVEIVYEGKAVEGAKHHKKKKKKKHKKKRRREHTNSPAASPIIITIDSDSDTPMVEDLPCNDHSSLVPENKTSIDSTNFMHSQSPPTSTTVSTTEVLSGERADSDSKASDLCSSNRHLDAATGILDGLHFDDSSDEQNLLPVQSPPNIPDLCEVEPLAAESSLPESDPILDNVPPTSTSEISELHKSFAESIFDFDLLNNVT

Target ORF information:

RefSeq Version	XM_002940264.5
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis topoisomerase I binding, arginine/serine-rich, E3 ubiquitin protein ligase (topors), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002940264.5

ORF Insert Sequence:

ATGAGGGAGA AGAGAACCAA GCGGCGGAGG CCGGGAGCGA GGCAAGAAGC GAATGACACG 
ATGATGGCAT CATCCTCTAA CAATTATTCA CTAGATGGCA GCTTTTCACC CCAGGCTGGC 
ACAAGCAAAC TACAAAACCA TGGTACTGCA ACAGATGCTT CCCCAGACTC AAAGTGTCCA 
ATATGTTTGG ACAGGTTTGA TAATGTGTCA CATCTTGATC GATGTCTGCA CAGATTTTGT 
TTCCGTTGCA TTCAAGAATG GGCAAAAAAC AAAGCTGAAT GCCCTCTATG CAAACAGCCC 
TTTTACTCCA TTTTCCATAG TGTCAAAGCA GAGGATGATT TTAAAGAATA TGTCTTGAGG 
CCCACAATGA ATGGTTCATT TGCCAGTCCT GATGGACATA GATTTCGTTA TCGCACAACT 
ATGAGTAGAG ACAATCATGT AGCCATTCGG ACATTAAGGT CTTCAAGCAC TCAAAGGACA 
TTTTCACCTC CTGACAATGG AATTTTATTT GAAGGGTTTT CAAATCAGAA TTTACATCAA 
AGAGGTGAAG AAATCCACCA GATGATAAGA AGGCTAGCCT CCAGACGACA AGCCAGTGCA 
GAGGGTCGTT CTATGAGGCA AATTCAAGAG CAAGAACTCA TTAACTTCAG AAGAGCTTTG 
TACCGGTCTG GAATACGTGT GAGAAACATT CAGGATGGCG GACGTTATAG AGATATTTCT 
GCTGAGTTCT TCCGGCGTAA CCCTGCTTGT CTTCATCGGC TAGTACCATG GTTGAAAAGA 
GAGTTGACTG TTCTCTTTGG TAGCCATGGT TCCCTTGTTA ACATTGTACA GCATATTATT 
ATGAGTAATG TCACACGATA TGATATGGAA AGTCAAGCAT TTGTTGAGGA TCTTCGTCCT 
TTTTTGCTTC ACCGCACAGA CCATTTCATA CATGAGTTTG TTAACTTTGC CCGATGCCCT 
TACAATATTG AAGCATATGA TCAGCATGCA AACTATGATT GTCCTGCTCC TTCATATGAG 
GAAGGAAGCC GTTCAGAGTC ATCTGTCATC ACAATATCGC CAGATGAAGC AGATACCAGA 
GATCCAATTA TTCCTTCTGC TGGTCCTGGT GTTGGCCAGA CAGCTTGGGA TGATGAGACC 
CCTGGTCCCT CTTATTCCAC ACTTGAGCAA GCCTCAGTTG CTGTATCCAC TACACTAGAT 
ATCTCAGAAA GCTCAGACGA TGAGCCACCC AGCATAAGAC AGACTTCACA AGAAGTTTCC 
AACAGTGAAC CCACAGAAAA CCTGCAGGAT CAGTCCTTTC CAACTGATGA CTGTGTTATA 
GTAGGGTATG TTAAACCACT GGCAGAAAGA ACTCCAGAAT TAGTTGAATT GTCATCCGAC 
TCCGAGAGCT CATTGAGTGA GGTTAAGACA GAAGATGCCA AAAAGCCTAC GATTAAACCA 
TTTTTCCTAA GTGACAGTAG TGAATTGAGC AGGTCATCAT CATCTTTGTA CACACCTGCA 
AAAGAAAAAC GTTCACGCAA AACCAAGGGA AAAACCACAT CTAGCAAACA TTCCCATTCC 
AGGAAAAAAG AGAAGGGAAA ATGTCGACTT GATTTAACTT CTAGAAAGGA TAAGGGGGAT 
TTGAACAGTA ATTATGCTTT GTCCAGATGG AGGGATAGGT CAAGGAGTTC TGATTGTTAT 
TCTCGTTCTT CTCGCAATAA AAGCCACAAC AGTCGAAGGA GGCATTGCAG CAAAAATAAT 
TTGAAACCCA GGAGTCCTGA AAAGAGCAGG CATAAAAGTA GGAAAGGAAG GAAGAAATCT 
AGTAGTAGGG ATCGAAGTCT GTCTTGGAGG AGTAGGACTG TGTCACTAAC TAGTGAAAGC 
TCCAGAGAGA GATGCGAGTC AAGCTCTAGA AGCCGAAATC GAAGTAGGGG TAGGTCACGA 
AGTCATGATA GTGACAATGA TTTTTCTTCC AACAACTATC GCAGTTCTTA TCAATGGGAA 
TATACCTATT ACAGTCGAAA TCGGGATAGA GATGGCTATG AACAGTCCTA CAGGAGACGG 
ACTTGTGGTC GAGGACGTTA TTCAAGACCC TCTGCTAGCC CAGAATATAA AATTCAGTCT 
TACTCTGAAA AGAAAGATAC AAGAAAGGAG AGGGGATACA TTGCTAGTAA ACAATATCGC 
CGTGAGCGAT ACAGGTCAAG GAGTCGCTCA AGCAGTCAGA TGAATACATC AGGGACTGAG 
CATACCAGAT CTGACAAACC AAGTGGAAAA AGAAAATACA AAACACACCA TTTGGAAAAG 
CAGGAAAGGG AAGGGAACAA TCGAGAAGTT CCTTCTGCCA AAGAAAAAGA GGGAGGTCTG 
CAAGCAGGCA AGACTTCTCT TGGCAATCAT ACTGGTAGTC TCAAAAATGA TTTAATGGGT 
TCATCCTCTG AACCTAAGCA GAAGTTAAGA AAAAAGGCAA GAAGCCCAAG TGTGGAAATA 
GTATATGAGG GCAAAGCAGT TGAAGGGGCA AAGCACCACA AAAAGAAGAA GAAAAAGAAG 
CACAAGAAAA AACGACGCAG GGAACACACT AATTCTCCAG CTGCTTCTCC CATCATTATC 
ACCATTGACA GTGACAGTGA CACCCCAATG GTGGAGGATC TCCCATGCAA TGACCACAGC 
TCCCTGGTAC CTGAAAACAA AACTAGTATT GACTCAACTA ACTTCATGCA CAGCCAGTCG 
CCACCTACCT CGACCACAGT AAGTACCACA GAAGTACTAA GTGGAGAAAG GGCAGACTCT 
GATTCCAAAG CCTCTGATTT ATGTTCAAGT AACAGGCACT TAGATGCTGC CACTGGAATA 
CTGGATGGTT TACATTTTGA TGATAGCTCT GATGAACAGA ATCTGCTCCC TGTACAAAGT 
CCTCCTAACA TACCGGATCT CTGTGAAGTA GAACCTTTAG CAGCTGAGAG TTCCTTGCCA 
GAGTCTGATC CTATACTGGA CAATGTACCA CCTACATCAA CTTCAGAAAT ATCTGAACTT 
CATAAAAGCT TTGCTGAGAG TATATTTGAC TTTGATCTCT TAAACAATGT AACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

topors ( XM_002940264.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002940310.1 Xenopus tropicalis topoisomerase I binding, arginine/serine-rich, E3 ubiquitin protein ligase (topors), mRNA.

Price & Availability↑

CloneID	OXa04122
Clone ID Related Accession (Same CDS sequence)	XM_002940264.5 , XM_002940264.4
Accession Version	XM_002940264.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3057bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	E3 ubiquitin-protein ligase Topors
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002940264.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGGAGA AGAGAACCAA GCGGCGGAGG CCGGGAGCGA GGCAAGAAGC GAATGACACG 
ATGATGGCAT CATCCTCTAA CAATTATTCA CTAGATGGCA GCTTTTCACC CCAGGCTGGC 
ACAAGCAAAC TACAAAACCA TGGTACTGCA ACAGATGCTT CCCCAGACTC AAAGTGTCCA 
ATATGTTTGG ACAGGTTTGA TAATGTGTCA CATCTTGATC GATGTCTGCA CAGATTTTGT 
TTCCGTTGCA TTCAAGAATG GGCAAAAAAC AAAGCTGAAT GCCCTCTATG CAAACAGCCC 
TTTTACTCCA TTTTCCATAG TGTCAAAGCA GAGGATGATT TTAAAGAATA TGTCTTGAGG 
CCCACAATGA ATGGTTCATT TGCCAGTCCT GATGGACATA GATTTCGTTA TCGCACAACT 
ATGAGTAGAG ACAATCATGT AGCCATTCGG ACATTAAGGT CTTCAAGCAC TCAAAGGACA 
TTTTCACCTC CTGACAATGG AATTTTATTT GAAGGGTTTT CAAATCAGAA TTTACATCAA 
AGAGGTGAAG AAATCCACCA GATGATAAGA AGGCTAGCCT CCAGACGACA AGCCAGTGCA 
GAGGGTCGTT CTATGAGGCA AATTCAAGAG CAAGAACTCA TTAACTTCAG AAGAGCTTTG 
TACCGGTCTG GAATACGTGT GAGAAACATT CAGGATGGCG GACGTTATAG AGATATTTCT 
GCTGAGTTCT TCCGGCGTAA CCCTGCTTGT CTTCATCGGC TAGTACCATG GTTGAAAAGA 
GAGTTGACTG TTCTCTTTGG TAGCCATGGT TCCCTTGTTA ACATTGTACA GCATATTATT 
ATGAGTAATG TCACACGATA TGATATGGAA AGTCAAGCAT TTGTTGAGGA TCTTCGTCCT 
TTTTTGCTTC ACCGCACAGA CCATTTCATA CATGAGTTTG TTAACTTTGC CCGATGCCCT 
TACAATATTG AAGCATATGA TCAGCATGCA AACTATGATT GTCCTGCTCC TTCATATGAG 
GAAGGAAGCC GTTCAGAGTC ATCTGTCATC ACAATATCGC CAGATGAAGC AGATACCAGA 
GATCCAATTA TTCCTTCTGC TGGTCCTGGT GTTGGCCAGA CAGCTTGGGA TGATGAGACC 
CCTGGTCCCT CTTATTCCAC ACTTGAGCAA GCCTCAGTTG CTGTATCCAC TACACTAGAT 
ATCTCAGAAA GCTCAGACGA TGAGCCACCC AGCATAAGAC AGACTTCACA AGAAGTTTCC 
AACAGTGAAC CCACAGAAAA CCTGCAGGAT CAGTCCTTTC CAACTGATGA CTGTGTTATA 
GTAGGGTATG TTAAACCACT GGCAGAAAGA ACTCCAGAAT TAGTTGAATT GTCATCCGAC 
TCCGAGAGCT CATTGAGTGA GGTTAAGACA GAAGATGCCA AAAAGCCTAC GATTAAACCA 
TTTTTCCTAA GTGACAGTAG TGAATTGAGC AGGTCATCAT CATCTTTGTA CACACCTGCA 
AAAGAAAAAC GTTCACGCAA AACCAAGGGA AAAACCACAT CTAGCAAACA TTCCCATTCC 
AGGAAAAAAG AGAAGGGAAA ATGTCGACTT GATTTAACTT CTAGAAAGGA TAAGGGGGAT 
TTGAACAGTA ATTATGCTTT GTCCAGATGG AGGGATAGGT CAAGGAGTTC TGATTGTTAT 
TCTCGTTCTT CTCGCAATAA AAGCCACAAC AGTCGAAGGA GGCATTGCAG CAAAAATAAT 
TTGAAACCCA GGAGTCCTGA AAAGAGCAGG CATAAAAGTA GGAAAGGAAG GAAGAAATCT 
AGTAGTAGGG ATCGAAGTCT GTCTTGGAGG AGTAGGACTG TGTCACTAAC TAGTGAAAGC 
TCCAGAGAGA GATGCGAGTC AAGCTCTAGA AGCCGAAATC GAAGTAGGGG TAGGTCACGA 
AGTCATGATA GTGACAATGA TTTTTCTTCC AACAACTATC GCAGTTCTTA TCAATGGGAA 
TATACCTATT ACAGTCGAAA TCGGGATAGA GATGGCTATG AACAGTCCTA CAGGAGACGG 
ACTTGTGGTC GAGGACGTTA TTCAAGACCC TCTGCTAGCC CAGAATATAA AATTCAGTCT 
TACTCTGAAA AGAAAGATAC AAGAAAGGAG AGGGGATACA TTGCTAGTAA ACAATATCGC 
CGTGAGCGAT ACAGGTCAAG GAGTCGCTCA AGCAGTCAGA TGAATACATC AGGGACTGAG 
CATACCAGAT CTGACAAACC AAGTGGAAAA AGAAAATACA AAACACACCA TTTGGAAAAG 
CAGGAAAGGG AAGGGAACAA TCGAGAAGTT CCTTCTGCCA AAGAAAAAGA GGGAGGTCTG 
CAAGCAGGCA AGACTTCTCT TGGCAATCAT ACTGGTAGTC TCAAAAATGA TTTAATGGGT 
TCATCCTCTG AACCTAAGCA GAAGTTAAGA AAAAAGGCAA GAAGCCCAAG TGTGGAAATA 
GTATATGAGG GCAAAGCAGT TGAAGGGGCA AAGCACCACA AAAAGAAGAA GAAAAAGAAG 
CACAAGAAAA AACGACGCAG GGAACACACT AATTCTCCAG CTGCTTCTCC CATCATTATC 
ACCATTGACA GTGACAGTGA CACCCCAATG GTGGAGGATC TCCCATGCAA TGACCACAGC 
TCCCTGGTAC CTGAAAACAA AACTAGTATT GACTCAACTA ACTTCATGCA CAGCCAGTCG 
CCACCTACCT CGACCACAGT AAGTACCACA GAAGTACTAA GTGGAGAAAG GGCAGACTCT 
GATTCCAAAG CCTCTGATTT ATGTTCAAGT AACAGGCACT TAGATGCTGC CACTGGAATA 
CTGGATGGTT TACATTTTGA TGATAGCTCT GATGAACAGA ATCTGCTCCC TGTACAAAGT 
CCTCCTAACA TACCGGATCT CTGTGAAGTA GAACCTTTAG CAGCTGAGAG TTCCTTGCCA 
GAGTCTGATC CTATACTGGA CAATGTACCA CCTACATCAA CTTCAGAAAT ATCTGAACTT 
CATAAAAGCT TTGCTGAGAG TATATTTGAC TTTGATCTCT TAAACAATGT AACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002940310.1
CDS	143..3199
Translation	MREKRTKRRRPGARQEANDTMMASSSNNYSLDGSFSPQAGTSKLQNHGTATDASPDSKCPICLDRFDNVSHLDRCLHRFCFRCIQEWAKNKAECPLCKQPFYSIFHSVKAEDDFKEYVLRPTMNGSFASPDGHRFRYRTTMSRDNHVAIRTLRSSSTQRTFSPPDNGILFEGFSNQNLHQRGEEIHQMIRRLASRRQASAEGRSMRQIQEQELINFRRALYRSGIRVRNIQDGGRYRDISAEFFRRNPACLHRLVPWLKRELTVLFGSHGSLVNIVQHIIMSNVTRYDMESQAFVEDLRPFLLHRTDHFIHEFVNFARCPYNIEAYDQHANYDCPAPSYEEGSRSESSVITISPDEADTRDPIIPSAGPGVGQTAWDDETPGPSYSTLEQASVAVSTTLDISESSDDEPPSIRQTSQEVSNSEPTENLQDQSFPTDDCVIVGYVKPLAERTPELVELSSDSESSLSEVKTEDAKKPTIKPFFLSDSSELSRSSSSLYTPAKEKRSRKTKGKTTSSKHSHSRKKEKGKCRLDLTSRKDKGDLNSNYALSRWRDRSRSSDCYSRSSRNKSHNSRRRHCSKNNLKPRSPEKSRHKSRKGRKKSSSRDRSLSWRSRTVSLTSESSRERCESSSRSRNRSRGRSRSHDSDNDFSSNNYRSSYQWEYTYYSRNRDRDGYEQSYRRRTCGRGRYSRPSASPEYKIQSYSEKKDTRKERGYIASKQYRRERYRSRSRSSSQMNTSGTEHTRSDKPSGKRKYKTHHLEKQEREGNNREVPSAKEKEGGLQAGKTSLGNHTGSLKNDLMGSSSEPKQKLRKKARSPSVEIVYEGKAVEGAKHHKKKKKKKHKKKRRREHTNSPAASPIIITIDSDSDTPMVEDLPCNDHSSLVPENKTSIDSTNFMHSQSPPTSTTVSTTEVLSGERADSDSKASDLCSSNRHLDAATGILDGLHFDDSSDEQNLLPVQSPPNIPDLCEVEPLAAESSLPESDPILDNVPPTSTSEISELHKSFAESIFDFDLLNNVT

Target ORF information:

RefSeq Version	XM_002940264.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis topoisomerase I binding, arginine/serine-rich, E3 ubiquitin protein ligase (topors), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002940264.4

ORF Insert Sequence:

ATGAGGGAGA AGAGAACCAA GCGGCGGAGG CCGGGAGCGA GGCAAGAAGC GAATGACACG 
ATGATGGCAT CATCCTCTAA CAATTATTCA CTAGATGGCA GCTTTTCACC CCAGGCTGGC 
ACAAGCAAAC TACAAAACCA TGGTACTGCA ACAGATGCTT CCCCAGACTC AAAGTGTCCA 
ATATGTTTGG ACAGGTTTGA TAATGTGTCA CATCTTGATC GATGTCTGCA CAGATTTTGT 
TTCCGTTGCA TTCAAGAATG GGCAAAAAAC AAAGCTGAAT GCCCTCTATG CAAACAGCCC 
TTTTACTCCA TTTTCCATAG TGTCAAAGCA GAGGATGATT TTAAAGAATA TGTCTTGAGG 
CCCACAATGA ATGGTTCATT TGCCAGTCCT GATGGACATA GATTTCGTTA TCGCACAACT 
ATGAGTAGAG ACAATCATGT AGCCATTCGG ACATTAAGGT CTTCAAGCAC TCAAAGGACA 
TTTTCACCTC CTGACAATGG AATTTTATTT GAAGGGTTTT CAAATCAGAA TTTACATCAA 
AGAGGTGAAG AAATCCACCA GATGATAAGA AGGCTAGCCT CCAGACGACA AGCCAGTGCA 
GAGGGTCGTT CTATGAGGCA AATTCAAGAG CAAGAACTCA TTAACTTCAG AAGAGCTTTG 
TACCGGTCTG GAATACGTGT GAGAAACATT CAGGATGGCG GACGTTATAG AGATATTTCT 
GCTGAGTTCT TCCGGCGTAA CCCTGCTTGT CTTCATCGGC TAGTACCATG GTTGAAAAGA 
GAGTTGACTG TTCTCTTTGG TAGCCATGGT TCCCTTGTTA ACATTGTACA GCATATTATT 
ATGAGTAATG TCACACGATA TGATATGGAA AGTCAAGCAT TTGTTGAGGA TCTTCGTCCT 
TTTTTGCTTC ACCGCACAGA CCATTTCATA CATGAGTTTG TTAACTTTGC CCGATGCCCT 
TACAATATTG AAGCATATGA TCAGCATGCA AACTATGATT GTCCTGCTCC TTCATATGAG 
GAAGGAAGCC GTTCAGAGTC ATCTGTCATC ACAATATCGC CAGATGAAGC AGATACCAGA 
GATCCAATTA TTCCTTCTGC TGGTCCTGGT GTTGGCCAGA CAGCTTGGGA TGATGAGACC 
CCTGGTCCCT CTTATTCCAC ACTTGAGCAA GCCTCAGTTG CTGTATCCAC TACACTAGAT 
ATCTCAGAAA GCTCAGACGA TGAGCCACCC AGCATAAGAC AGACTTCACA AGAAGTTTCC 
AACAGTGAAC CCACAGAAAA CCTGCAGGAT CAGTCCTTTC CAACTGATGA CTGTGTTATA 
GTAGGGTATG TTAAACCACT GGCAGAAAGA ACTCCAGAAT TAGTTGAATT GTCATCCGAC 
TCCGAGAGCT CATTGAGTGA GGTTAAGACA GAAGATGCCA AAAAGCCTAC GATTAAACCA 
TTTTTCCTAA GTGACAGTAG TGAATTGAGC AGGTCATCAT CATCTTTGTA CACACCTGCA 
AAAGAAAAAC GTTCACGCAA AACCAAGGGA AAAACCACAT CTAGCAAACA TTCCCATTCC 
AGGAAAAAAG AGAAGGGAAA ATGTCGACTT GATTTAACTT CTAGAAAGGA TAAGGGGGAT 
TTGAACAGTA ATTATGCTTT GTCCAGATGG AGGGATAGGT CAAGGAGTTC TGATTGTTAT 
TCTCGTTCTT CTCGCAATAA AAGCCACAAC AGTCGAAGGA GGCATTGCAG CAAAAATAAT 
TTGAAACCCA GGAGTCCTGA AAAGAGCAGG CATAAAAGTA GGAAAGGAAG GAAGAAATCT 
AGTAGTAGGG ATCGAAGTCT GTCTTGGAGG AGTAGGACTG TGTCACTAAC TAGTGAAAGC 
TCCAGAGAGA GATGCGAGTC AAGCTCTAGA AGCCGAAATC GAAGTAGGGG TAGGTCACGA 
AGTCATGATA GTGACAATGA TTTTTCTTCC AACAACTATC GCAGTTCTTA TCAATGGGAA 
TATACCTATT ACAGTCGAAA TCGGGATAGA GATGGCTATG AACAGTCCTA CAGGAGACGG 
ACTTGTGGTC GAGGACGTTA TTCAAGACCC TCTGCTAGCC CAGAATATAA AATTCAGTCT 
TACTCTGAAA AGAAAGATAC AAGAAAGGAG AGGGGATACA TTGCTAGTAA ACAATATCGC 
CGTGAGCGAT ACAGGTCAAG GAGTCGCTCA AGCAGTCAGA TGAATACATC AGGGACTGAG 
CATACCAGAT CTGACAAACC AAGTGGAAAA AGAAAATACA AAACACACCA TTTGGAAAAG 
CAGGAAAGGG AAGGGAACAA TCGAGAAGTT CCTTCTGCCA AAGAAAAAGA GGGAGGTCTG 
CAAGCAGGCA AGACTTCTCT TGGCAATCAT ACTGGTAGTC TCAAAAATGA TTTAATGGGT 
TCATCCTCTG AACCTAAGCA GAAGTTAAGA AAAAAGGCAA GAAGCCCAAG TGTGGAAATA 
GTATATGAGG GCAAAGCAGT TGAAGGGGCA AAGCACCACA AAAAGAAGAA GAAAAAGAAG 
CACAAGAAAA AACGACGCAG GGAACACACT AATTCTCCAG CTGCTTCTCC CATCATTATC 
ACCATTGACA GTGACAGTGA CACCCCAATG GTGGAGGATC TCCCATGCAA TGACCACAGC 
TCCCTGGTAC CTGAAAACAA AACTAGTATT GACTCAACTA ACTTCATGCA CAGCCAGTCG 
CCACCTACCT CGACCACAGT AAGTACCACA GAAGTACTAA GTGGAGAAAG GGCAGACTCT 
GATTCCAAAG CCTCTGATTT ATGTTCAAGT AACAGGCACT TAGATGCTGC CACTGGAATA 
CTGGATGGTT TACATTTTGA TGATAGCTCT GATGAACAGA ATCTGCTCCC TGTACAAAGT 
CCTCCTAACA TACCGGATCT CTGTGAAGTA GAACCTTTAG CAGCTGAGAG TTCCTTGCCA 
GAGTCTGATC CTATACTGGA CAATGTACCA CCTACATCAA CTTCAGAAAT ATCTGAACTT 
CATAAAAGCT TTGCTGAGAG TATATTTGAC TTTGATCTCT TAAACAATGT AACATAG