mid1 gene cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

mRNA	Protein	Name
XM_012956768.3	XP_012812222.1	E3 ubiquitin-protein ligase Midline-1 isoform X1
XM_012956769.3	XP_012812223.1	E3 ubiquitin-protein ligase Midline-1 isoform X1
XM_012956768.2	XP_012812222.1	E3 ubiquitin-protein ligase Midline-1 isoform X1
XM_012956770.2	XP_012812224.1	E3 ubiquitin-protein ligase Midline-1 isoform X1
XM_012956770.3	XP_012812224.1	E3 ubiquitin-protein ligase Midline-1 isoform X1
XM_012956771.3	XP_012812225.1	E3 ubiquitin-protein ligase Midline-1 isoform X1
XM_012956769.2	XP_012812223.1	E3 ubiquitin-protein ligase Midline-1 isoform X1
XM_012956771.2	XP_012812225.1	E3 ubiquitin-protein ligase Midline-1 isoform X1
XM_018091174.1	XP_017946663.1	E3 ubiquitin-protein ligase Midline-1 isoform X2
NM_001006875.1	NP_001006876.1	E3 ubiquitin-protein ligase Midline-1

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa02239	NM_001006875.1 Latest version!	Xenopus tropicalis midline 1 (mid1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OXa02239	XM_012956771.3 Latest version!	Xenopus tropicalis midline 1 (mid1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OXa02239	XM_012956769.3 Latest version!	Xenopus tropicalis midline 1 (mid1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OXa02239	XM_012956770.3 Latest version!	Xenopus tropicalis midline 1 (mid1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OXa02239	XM_012956768.3 Latest version!	Xenopus tropicalis midline 1 (mid1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OXa42507	XM_018091174.1 Latest version!	Xenopus tropicalis midline 1 (mid1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
View Old Accession Versions >>
OXa02239	XM_012956768.2	Xenopus tropicalis midline 1 (mid1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OXa02239	XM_012956770.2	Xenopus tropicalis midline 1 (mid1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OXa02239	XM_012956769.2	Xenopus tropicalis midline 1 (mid1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OXa02239	XM_012956771.2	Xenopus tropicalis midline 1 (mid1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
Hide Old Accession Versions >>

mid1 ( NM_001006875.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> NP_001006876.1 Xenopus tropicalis midline 1 (mid1), mRNA.

Price & Availability↑

CloneID	OXa02239
Clone ID Related Accession (Same CDS sequence)	XM_012956768.3 , XM_012956769.3 , XM_012956768.2 , XM_012956770.2 , XM_012956770.3 , XM_012956771.3 , XM_012956769.2 , XM_012956771.2 , NM_001006875.1
Accession Version	NM_001006875.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2007bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-21
Organism	Xenopus tropicalis(tropical clawed frog)
Product	E3 ubiquitin-protein ligase Midline-1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC076998.1. ##Evidence-Data-START## Transcript exon combination :: BC076998.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00028292, SAMD00028293 [ECO:0000348] ##Evidence-Data-END##

ATGGAAACAC TGGAGTCTGA ACTGACCTGC CCTATCTGTC TGGAGCTGTT TGAAGATCCA 
CTGCTGCTGC CTTGTGCTCA CAGCCTGTGT TTTAACTGTG CGCATCGCAT CCTTGTCTCC 
CACTGTGCCA ACAACGAATC CGTAGAATCT ATTAACGCCT TCCAGTGCCC CACCTGCCGT 
TATGTCATCA CACTCAGCAA GCGTGGCTTA GAGGGGCTAA AGAGGAACGT GACTTTACAG 
AACATCATTG ACAGATTTCA GAAAGCCTCA GTGAGCGGGC CCAATTCACC TAGTGAAACG 
CGTCGAGAAC GTGCTTACGA TACCACTAAC AGCATGACAT CAAGCGAAAA AGTACTCTGC 
CAGTTCTGTG ATCAGGACCA GGCAAGGGAG GCTGTGAAAA CCTGTGTGAC CTGTGAAGTT 
TCATATTGTG AGGACTGTTT AAAAGCTACT CACCCAAACA AGAAACCATT CACTGGTCAC 
CGACTAATTG AACCCATCCC AGATTCTCAC ATAAGGGGGT TAATGTGCCT GGAACATGAG 
GATGAAAAAG TTAATATGTA TTGTGTGACA GATGACCAGC TAATCTGTGC CTTGTGTAAA 
TTGGTTGGGA GGCATCGAGA TCATCAAGTT GCTGCCCTTG GTGACAGATA CGACAAACTA 
AAGCAAAGCC TGGAAAGCAA CCTTACCAAC CTCATCAAAA GGAACACAGA AATGGAAATT 
CTGTTGTCCA AGCTCATTCA GACCTGCCAG CAGGTGGAGG TTAATGCATC ACGACAAGAA 
GCAAAATTAA TGGAGGAGAG TGACCAACTG ATTGAAATTA TTCAACAGAG AAGACAAATA 
ATTGGAACAA AAATAAAAGA AGGAAAGGTC GTGAGGTTAA GGAAACTGGC CCAGCAGGTT 
GCCAACTGTA AGCAGTGCAT CGAGCGCTCC ACATCCCTGA TCTCACAGGC AGAACAGTCT 
CTAAAGGAAA ACGATCACGC TCGCTTCCTT CAGTCAGCCA AGAATATCAC AGAAAGAGTC 
TCAATGGCAA CAGCATCTTC TCAAGTGCTT ATTCCAGAAA TCAACCTGAA TGACACATTT 
GACACATTTG CATTGGATTT TTCAAGAGAG AAGAAGCTGT TAGAATGTCT TGATTATCTG 
ACAGCTCCCA ATCCCCCTGT AATCAGAGAG GAACTCTGCA CTGCATCCTA CGACACCATT 
ACTGTGCATT GGACATCTGA GGATGAGTTT AGCGTGGTTT CCTATGAGCT GCAGTATACC 
ATCTTCACTG GACAAGCTAA CGTTGTTAGT TTGTGTAACT CAGCCGACAG CTGGATGATT 
GTTCCCAATA TCAAACAGAA CCATTACACA GTTCATGGTC TCCAGAGTGG CACCAAGTAC 
ATCTTCATTG TTAAGGCCAT AAACCAAGCT GGAAGTCGCA ACAGTGAACC TGGCAAGCTA 
AAAACCAACA GTCAACCTTT TAAATTAGAT CCCAAGTCTG CTCACAGGAA ACTTAAGGTA 
TCACATGACA ACCTGACCGT AGAGAGGGAC GAAACCTCTT CAAAAAAGAG CCACACACCA 
GAGCGTTTTA CAAGCCAAGG AAGTTATGGT GTTGCTGGCA ACGTGTTCCT CGACAGTGGC 
CGGCATTATT GGGAAGTGGT TATAAGCGGA AGCACCTGGT ATGCCATTGG CATTGCCTAC 
AAATCTGCAC CCAAGCACGA GTGGATAGGG AAGAATACAG CCTCTTGGGT GTTTTGCCGT 
TGTAACAACA ACTGGGCTGT AAGACACAAC AGTAAAGAGA TTCCTATAGA GCCATCTCCG 
CATCTTCGCC GCGTTGGGAT CTTGCTGGAC TACGACAATG GCTCATTGTC TTTTTATGAT 
GCTTTGAACT CCATTCACCT CTACACTTTT GACATTGCTT TTGCACAGCC CGTGTGCCCA 
ACCCTCACCG TGTGGAATAA GTGCTTAACA ATTTTAACAG GTCTCCCCAT ACCTGATCAC 
TTGGACTGCC TAGAGCAACT AGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001006876.1
CDS	147..2153
Translation	METLESELTCPICLELFEDPLLLPCAHSLCFNCAHRILVSHCANNESVESINAFQCPTCRYVITLSKRGLEGLKRNVTLQNIIDRFQKASVSGPNSPSETRRERAYDTTNSMTSSEKVLCQFCDQDQAREAVKTCVTCEVSYCEDCLKATHPNKKPFTGHRLIEPIPDSHIRGLMCLEHEDEKVNMYCVTDDQLICALCKLVGRHRDHQVAALGDRYDKLKQSLESNLTNLIKRNTEMEILLSKLIQTCQQVEVNASRQEAKLMEESDQLIEIIQQRRQIIGTKIKEGKVVRLRKLAQQVANCKQCIERSTSLISQAEQSLKENDHARFLQSAKNITERVSMATASSQVLIPEINLNDTFDTFALDFSREKKLLECLDYLTAPNPPVIREELCTASYDTITVHWTSEDEFSVVSYELQYTIFTGQANVVSLCNSADSWMIVPNIKQNHYTVHGLQSGTKYIFIVKAINQAGSRNSEPGKLKTNSQPFKLDPKSAHRKLKVSHDNLTVERDETSSKKSHTPERFTSQGSYGVAGNVFLDSGRHYWEVVISGSTWYAIGIAYKSAPKHEWIGKNTASWVFCRCNNNWAVRHNSKEIPIEPSPHLRRVGILLDYDNGSLSFYDALNSIHLYTFDIAFAQPVCPTLTVWNKCLTILTGLPIPDHLDCLEQLA

Target ORF information:

RefSeq Version	NM_001006875.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis midline 1 (mid1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001006875.1

ORF Insert Sequence:

ATGGAAACAC TGGAGTCTGA ACTGACCTGC CCTATCTGTC TGGAGCTGTT TGAAGATCCA 
CTGCTGCTGC CTTGTGCTCA CAGCCTGTGT TTTAACTGTG CGCATCGCAT CCTTGTCTCC 
CACTGTGCCA ACAACGAATC CGTAGAATCT ATTAACGCCT TCCAGTGCCC CACCTGCCGT 
TATGTCATCA CACTCAGCAA GCGTGGCTTA GAGGGGCTAA AGAGGAACGT GACTTTACAG 
AACATCATTG ACAGATTTCA GAAAGCCTCA GTGAGCGGGC CCAATTCACC TAGTGAAACG 
CGTCGAGAAC GTGCTTACGA TACCACTAAC AGCATGACAT CAAGCGAAAA AGTACTCTGC 
CAGTTCTGTG ATCAGGACCA GGCAAGGGAG GCTGTGAAAA CCTGTGTGAC CTGTGAAGTT 
TCATATTGTG AGGACTGTTT AAAAGCTACT CACCCAAACA AGAAACCATT CACTGGTCAC 
CGACTAATTG AACCCATCCC AGATTCTCAC ATAAGGGGGT TAATGTGCCT GGAACATGAG 
GATGAAAAAG TTAATATGTA TTGTGTGACA GATGACCAGC TAATCTGTGC CTTGTGTAAA 
TTGGTTGGGA GGCATCGAGA TCATCAAGTT GCTGCCCTTG GTGACAGATA CGACAAACTA 
AAGCAAAGCC TGGAAAGCAA CCTTACCAAC CTCATCAAAA GGAACACAGA AATGGAAATT 
CTGTTGTCCA AGCTCATTCA GACCTGCCAG CAGGTGGAGG TTAATGCATC ACGACAAGAA 
GCAAAATTAA TGGAGGAGAG TGACCAACTG ATTGAAATTA TTCAACAGAG AAGACAAATA 
ATTGGAACAA AAATAAAAGA AGGAAAGGTC GTGAGGTTAA GGAAACTGGC CCAGCAGGTT 
GCCAACTGTA AGCAGTGCAT CGAGCGCTCC ACATCCCTGA TCTCACAGGC AGAACAGTCT 
CTAAAGGAAA ACGATCACGC TCGCTTCCTT CAGTCAGCCA AGAATATCAC AGAAAGAGTC 
TCAATGGCAA CAGCATCTTC TCAAGTGCTT ATTCCAGAAA TCAACCTGAA TGACACATTT 
GACACATTTG CATTGGATTT TTCAAGAGAG AAGAAGCTGT TAGAATGTCT TGATTATCTG 
ACAGCTCCCA ATCCCCCTGT AATCAGAGAG GAACTCTGCA CTGCATCCTA CGACACCATT 
ACTGTGCATT GGACATCTGA GGATGAGTTT AGCGTGGTTT CCTATGAGCT GCAGTATACC 
ATCTTCACTG GACAAGCTAA CGTTGTTAGT TTGTGTAACT CAGCCGACAG CTGGATGATT 
GTTCCCAATA TCAAACAGAA CCATTACACA GTTCATGGTC TCCAGAGTGG CACCAAGTAC 
ATCTTCATTG TTAAGGCCAT AAACCAAGCT GGAAGTCGCA ACAGTGAACC TGGCAAGCTA 
AAAACCAACA GTCAACCTTT TAAATTAGAT CCCAAGTCTG CTCACAGGAA ACTTAAGGTA 
TCACATGACA ACCTGACCGT AGAGAGGGAC GAAACCTCTT CAAAAAAGAG CCACACACCA 
GAGCGTTTTA CAAGCCAAGG AAGTTATGGT GTTGCTGGCA ACGTGTTCCT CGACAGTGGC 
CGGCATTATT GGGAAGTGGT TATAAGCGGA AGCACCTGGT ATGCCATTGG CATTGCCTAC 
AAATCTGCAC CCAAGCACGA GTGGATAGGG AAGAATACAG CCTCTTGGGT GTTTTGCCGT 
TGTAACAACA ACTGGGCTGT AAGACACAAC AGTAAAGAGA TTCCTATAGA GCCATCTCCG 
CATCTTCGCC GCGTTGGGAT CTTGCTGGAC TACGACAATG GCTCATTGTC TTTTTATGAT 
GCTTTGAACT CCATTCACCT CTACACTTTT GACATTGCTT TTGCACAGCC CGTGTGCCCA 
ACCCTCACCG TGTGGAATAA GTGCTTAACA ATTTTAACAG GTCTCCCCAT ACCTGATCAC 
TTGGACTGCC TAGAGCAACT AGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mid1 ( XM_012956768.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012812222.1 Xenopus tropicalis midline 1 (mid1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa02239
Clone ID Related Accession (Same CDS sequence)	XM_012956768.3 , XM_012956769.3 , XM_012956768.2 , XM_012956770.2 , XM_012956770.3 , XM_012956771.3 , XM_012956769.2 , XM_012956771.2 , NM_001006875.1
Accession Version	XM_012956768.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2007bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	E3 ubiquitin-protein ligase Midline-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012956768.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAACAC TGGAGTCTGA ACTGACCTGC CCTATCTGTC TGGAGCTGTT TGAAGATCCA 
CTGCTGCTGC CTTGTGCTCA CAGCCTGTGT TTTAACTGTG CGCATCGCAT CCTTGTCTCC 
CACTGTGCCA ACAACGAATC CGTAGAATCT ATTAACGCCT TCCAGTGCCC CACCTGCCGT 
TATGTCATCA CACTCAGCAA GCGTGGCTTA GAGGGGCTAA AGAGGAACGT GACTTTACAG 
AACATCATTG ACAGATTTCA GAAAGCCTCA GTGAGCGGGC CCAATTCACC TAGTGAAACG 
CGTCGAGAAC GTGCTTACGA TACCACTAAC AGCATGACAT CAAGCGAAAA AGTACTCTGC 
CAGTTCTGTG ATCAGGACCA GGCAAGGGAG GCTGTGAAAA CCTGTGTGAC CTGTGAAGTT 
TCATATTGTG AGGACTGTTT AAAAGCTACT CACCCAAACA AGAAACCATT CACTGGTCAC 
CGACTAATTG AACCCATCCC AGATTCTCAC ATAAGGGGGT TAATGTGCCT GGAACATGAG 
GATGAAAAAG TTAATATGTA TTGTGTGACA GATGACCAGC TAATCTGTGC CTTGTGTAAA 
TTGGTTGGGA GGCATCGAGA TCATCAAGTT GCTGCCCTTG GTGACAGATA CGACAAACTA 
AAGCAAAGCC TGGAAAGCAA CCTTACCAAC CTCATCAAAA GGAACACAGA AATGGAAATT 
CTGTTGTCCA AGCTCATTCA GACCTGCCAG CAGGTGGAGG TTAATGCATC ACGACAAGAA 
GCAAAATTAA TGGAGGAGAG TGACCAACTG ATTGAAATTA TTCAACAGAG AAGACAAATA 
ATTGGAACAA AAATAAAAGA AGGAAAGGTC GTGAGGTTAA GGAAACTGGC CCAGCAGGTT 
GCCAACTGTA AGCAGTGCAT CGAGCGCTCC ACATCCCTGA TCTCACAGGC AGAACAGTCT 
CTAAAGGAAA ACGATCACGC TCGCTTCCTT CAGTCAGCCA AGAATATCAC AGAAAGAGTC 
TCAATGGCAA CAGCATCTTC TCAAGTGCTT ATTCCAGAAA TCAACCTGAA TGACACATTT 
GACACATTTG CATTGGATTT TTCAAGAGAG AAGAAGCTGT TAGAATGTCT TGATTATCTG 
ACAGCTCCCA ATCCCCCTGT AATCAGAGAG GAACTCTGCA CTGCATCCTA CGACACCATT 
ACTGTGCATT GGACATCTGA GGATGAGTTT AGCGTGGTTT CCTATGAGCT GCAGTATACC 
ATCTTCACTG GACAAGCTAA CGTTGTTAGT TTGTGTAACT CAGCCGACAG CTGGATGATT 
GTTCCCAATA TCAAACAGAA CCATTACACA GTTCATGGTC TCCAGAGTGG CACCAAGTAC 
ATCTTCATTG TTAAGGCCAT AAACCAAGCT GGAAGTCGCA ACAGTGAACC TGGCAAGCTA 
AAAACCAACA GTCAACCTTT TAAATTAGAT CCCAAGTCTG CTCACAGGAA ACTTAAGGTA 
TCACATGACA ACCTGACCGT AGAGAGGGAC GAAACCTCTT CAAAAAAGAG CCACACACCA 
GAGCGTTTTA CAAGCCAAGG AAGTTATGGT GTTGCTGGCA ACGTGTTCCT CGACAGTGGC 
CGGCATTATT GGGAAGTGGT TATAAGCGGA AGCACCTGGT ATGCCATTGG CATTGCCTAC 
AAATCTGCAC CCAAGCACGA GTGGATAGGG AAGAATACAG CCTCTTGGGT GTTTTGCCGT 
TGTAACAACA ACTGGGCTGT AAGACACAAC AGTAAAGAGA TTCCTATAGA GCCATCTCCG 
CATCTTCGCC GCGTTGGGAT CTTGCTGGAC TACGACAATG GCTCATTGTC TTTTTATGAT 
GCTTTGAACT CCATTCACCT CTACACTTTT GACATTGCTT TTGCACAGCC CGTGTGCCCA 
ACCCTCACCG TGTGGAATAA GTGCTTAACA ATTTTAACAG GTCTCCCCAT ACCTGATCAC 
TTGGACTGCC TAGAGCAACT AGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012812222.1
CDS	362..2368
Translation	METLESELTCPICLELFEDPLLLPCAHSLCFNCAHRILVSHCANNESVESINAFQCPTCRYVITLSKRGLEGLKRNVTLQNIIDRFQKASVSGPNSPSETRRERAYDTTNSMTSSEKVLCQFCDQDQAREAVKTCVTCEVSYCEDCLKATHPNKKPFTGHRLIEPIPDSHIRGLMCLEHEDEKVNMYCVTDDQLICALCKLVGRHRDHQVAALGDRYDKLKQSLESNLTNLIKRNTEMEILLSKLIQTCQQVEVNASRQEAKLMEESDQLIEIIQQRRQIIGTKIKEGKVVRLRKLAQQVANCKQCIERSTSLISQAEQSLKENDHARFLQSAKNITERVSMATASSQVLIPEINLNDTFDTFALDFSREKKLLECLDYLTAPNPPVIREELCTASYDTITVHWTSEDEFSVVSYELQYTIFTGQANVVSLCNSADSWMIVPNIKQNHYTVHGLQSGTKYIFIVKAINQAGSRNSEPGKLKTNSQPFKLDPKSAHRKLKVSHDNLTVERDETSSKKSHTPERFTSQGSYGVAGNVFLDSGRHYWEVVISGSTWYAIGIAYKSAPKHEWIGKNTASWVFCRCNNNWAVRHNSKEIPIEPSPHLRRVGILLDYDNGSLSFYDALNSIHLYTFDIAFAQPVCPTLTVWNKCLTILTGLPIPDHLDCLEQLA

Target ORF information:

RefSeq Version	XM_012956768.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis midline 1 (mid1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012956768.2

ORF Insert Sequence:

ATGGAAACAC TGGAGTCTGA ACTGACCTGC CCTATCTGTC TGGAGCTGTT TGAAGATCCA 
CTGCTGCTGC CTTGTGCTCA CAGCCTGTGT TTTAACTGTG CGCATCGCAT CCTTGTCTCC 
CACTGTGCCA ACAACGAATC CGTAGAATCT ATTAACGCCT TCCAGTGCCC CACCTGCCGT 
TATGTCATCA CACTCAGCAA GCGTGGCTTA GAGGGGCTAA AGAGGAACGT GACTTTACAG 
AACATCATTG ACAGATTTCA GAAAGCCTCA GTGAGCGGGC CCAATTCACC TAGTGAAACG 
CGTCGAGAAC GTGCTTACGA TACCACTAAC AGCATGACAT CAAGCGAAAA AGTACTCTGC 
CAGTTCTGTG ATCAGGACCA GGCAAGGGAG GCTGTGAAAA CCTGTGTGAC CTGTGAAGTT 
TCATATTGTG AGGACTGTTT AAAAGCTACT CACCCAAACA AGAAACCATT CACTGGTCAC 
CGACTAATTG AACCCATCCC AGATTCTCAC ATAAGGGGGT TAATGTGCCT GGAACATGAG 
GATGAAAAAG TTAATATGTA TTGTGTGACA GATGACCAGC TAATCTGTGC CTTGTGTAAA 
TTGGTTGGGA GGCATCGAGA TCATCAAGTT GCTGCCCTTG GTGACAGATA CGACAAACTA 
AAGCAAAGCC TGGAAAGCAA CCTTACCAAC CTCATCAAAA GGAACACAGA AATGGAAATT 
CTGTTGTCCA AGCTCATTCA GACCTGCCAG CAGGTGGAGG TTAATGCATC ACGACAAGAA 
GCAAAATTAA TGGAGGAGAG TGACCAACTG ATTGAAATTA TTCAACAGAG AAGACAAATA 
ATTGGAACAA AAATAAAAGA AGGAAAGGTC GTGAGGTTAA GGAAACTGGC CCAGCAGGTT 
GCCAACTGTA AGCAGTGCAT CGAGCGCTCC ACATCCCTGA TCTCACAGGC AGAACAGTCT 
CTAAAGGAAA ACGATCACGC TCGCTTCCTT CAGTCAGCCA AGAATATCAC AGAAAGAGTC 
TCAATGGCAA CAGCATCTTC TCAAGTGCTT ATTCCAGAAA TCAACCTGAA TGACACATTT 
GACACATTTG CATTGGATTT TTCAAGAGAG AAGAAGCTGT TAGAATGTCT TGATTATCTG 
ACAGCTCCCA ATCCCCCTGT AATCAGAGAG GAACTCTGCA CTGCATCCTA CGACACCATT 
ACTGTGCATT GGACATCTGA GGATGAGTTT AGCGTGGTTT CCTATGAGCT GCAGTATACC 
ATCTTCACTG GACAAGCTAA CGTTGTTAGT TTGTGTAACT CAGCCGACAG CTGGATGATT 
GTTCCCAATA TCAAACAGAA CCATTACACA GTTCATGGTC TCCAGAGTGG CACCAAGTAC 
ATCTTCATTG TTAAGGCCAT AAACCAAGCT GGAAGTCGCA ACAGTGAACC TGGCAAGCTA 
AAAACCAACA GTCAACCTTT TAAATTAGAT CCCAAGTCTG CTCACAGGAA ACTTAAGGTA 
TCACATGACA ACCTGACCGT AGAGAGGGAC GAAACCTCTT CAAAAAAGAG CCACACACCA 
GAGCGTTTTA CAAGCCAAGG AAGTTATGGT GTTGCTGGCA ACGTGTTCCT CGACAGTGGC 
CGGCATTATT GGGAAGTGGT TATAAGCGGA AGCACCTGGT ATGCCATTGG CATTGCCTAC 
AAATCTGCAC CCAAGCACGA GTGGATAGGG AAGAATACAG CCTCTTGGGT GTTTTGCCGT 
TGTAACAACA ACTGGGCTGT AAGACACAAC AGTAAAGAGA TTCCTATAGA GCCATCTCCG 
CATCTTCGCC GCGTTGGGAT CTTGCTGGAC TACGACAATG GCTCATTGTC TTTTTATGAT 
GCTTTGAACT CCATTCACCT CTACACTTTT GACATTGCTT TTGCACAGCC CGTGTGCCCA 
ACCCTCACCG TGTGGAATAA GTGCTTAACA ATTTTAACAG GTCTCCCCAT ACCTGATCAC 
TTGGACTGCC TAGAGCAACT AGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mid1 ( XM_012956770.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012812224.1 Xenopus tropicalis midline 1 (mid1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OXa02239
Clone ID Related Accession (Same CDS sequence)	XM_012956768.3 , XM_012956769.3 , XM_012956768.2 , XM_012956770.2 , XM_012956770.3 , XM_012956771.3 , XM_012956769.2 , XM_012956771.2 , NM_001006875.1
Accession Version	XM_012956770.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2007bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	E3 ubiquitin-protein ligase Midline-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012956770.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAACAC TGGAGTCTGA ACTGACCTGC CCTATCTGTC TGGAGCTGTT TGAAGATCCA 
CTGCTGCTGC CTTGTGCTCA CAGCCTGTGT TTTAACTGTG CGCATCGCAT CCTTGTCTCC 
CACTGTGCCA ACAACGAATC CGTAGAATCT ATTAACGCCT TCCAGTGCCC CACCTGCCGT 
TATGTCATCA CACTCAGCAA GCGTGGCTTA GAGGGGCTAA AGAGGAACGT GACTTTACAG 
AACATCATTG ACAGATTTCA GAAAGCCTCA GTGAGCGGGC CCAATTCACC TAGTGAAACG 
CGTCGAGAAC GTGCTTACGA TACCACTAAC AGCATGACAT CAAGCGAAAA AGTACTCTGC 
CAGTTCTGTG ATCAGGACCA GGCAAGGGAG GCTGTGAAAA CCTGTGTGAC CTGTGAAGTT 
TCATATTGTG AGGACTGTTT AAAAGCTACT CACCCAAACA AGAAACCATT CACTGGTCAC 
CGACTAATTG AACCCATCCC AGATTCTCAC ATAAGGGGGT TAATGTGCCT GGAACATGAG 
GATGAAAAAG TTAATATGTA TTGTGTGACA GATGACCAGC TAATCTGTGC CTTGTGTAAA 
TTGGTTGGGA GGCATCGAGA TCATCAAGTT GCTGCCCTTG GTGACAGATA CGACAAACTA 
AAGCAAAGCC TGGAAAGCAA CCTTACCAAC CTCATCAAAA GGAACACAGA AATGGAAATT 
CTGTTGTCCA AGCTCATTCA GACCTGCCAG CAGGTGGAGG TTAATGCATC ACGACAAGAA 
GCAAAATTAA TGGAGGAGAG TGACCAACTG ATTGAAATTA TTCAACAGAG AAGACAAATA 
ATTGGAACAA AAATAAAAGA AGGAAAGGTC GTGAGGTTAA GGAAACTGGC CCAGCAGGTT 
GCCAACTGTA AGCAGTGCAT CGAGCGCTCC ACATCCCTGA TCTCACAGGC AGAACAGTCT 
CTAAAGGAAA ACGATCACGC TCGCTTCCTT CAGTCAGCCA AGAATATCAC AGAAAGAGTC 
TCAATGGCAA CAGCATCTTC TCAAGTGCTT ATTCCAGAAA TCAACCTGAA TGACACATTT 
GACACATTTG CATTGGATTT TTCAAGAGAG AAGAAGCTGT TAGAATGTCT TGATTATCTG 
ACAGCTCCCA ATCCCCCTGT AATCAGAGAG GAACTCTGCA CTGCATCCTA CGACACCATT 
ACTGTGCATT GGACATCTGA GGATGAGTTT AGCGTGGTTT CCTATGAGCT GCAGTATACC 
ATCTTCACTG GACAAGCTAA CGTTGTTAGT TTGTGTAACT CAGCCGACAG CTGGATGATT 
GTTCCCAATA TCAAACAGAA CCATTACACA GTTCATGGTC TCCAGAGTGG CACCAAGTAC 
ATCTTCATTG TTAAGGCCAT AAACCAAGCT GGAAGTCGCA ACAGTGAACC TGGCAAGCTA 
AAAACCAACA GTCAACCTTT TAAATTAGAT CCCAAGTCTG CTCACAGGAA ACTTAAGGTA 
TCACATGACA ACCTGACCGT AGAGAGGGAC GAAACCTCTT CAAAAAAGAG CCACACACCA 
GAGCGTTTTA CAAGCCAAGG AAGTTATGGT GTTGCTGGCA ACGTGTTCCT CGACAGTGGC 
CGGCATTATT GGGAAGTGGT TATAAGCGGA AGCACCTGGT ATGCCATTGG CATTGCCTAC 
AAATCTGCAC CCAAGCACGA GTGGATAGGG AAGAATACAG CCTCTTGGGT GTTTTGCCGT 
TGTAACAACA ACTGGGCTGT AAGACACAAC AGTAAAGAGA TTCCTATAGA GCCATCTCCG 
CATCTTCGCC GCGTTGGGAT CTTGCTGGAC TACGACAATG GCTCATTGTC TTTTTATGAT 
GCTTTGAACT CCATTCACCT CTACACTTTT GACATTGCTT TTGCACAGCC CGTGTGCCCA 
ACCCTCACCG TGTGGAATAA GTGCTTAACA ATTTTAACAG GTCTCCCCAT ACCTGATCAC 
TTGGACTGCC TAGAGCAACT AGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012812224.1
CDS	221..2227
Translation	METLESELTCPICLELFEDPLLLPCAHSLCFNCAHRILVSHCANNESVESINAFQCPTCRYVITLSKRGLEGLKRNVTLQNIIDRFQKASVSGPNSPSETRRERAYDTTNSMTSSEKVLCQFCDQDQAREAVKTCVTCEVSYCEDCLKATHPNKKPFTGHRLIEPIPDSHIRGLMCLEHEDEKVNMYCVTDDQLICALCKLVGRHRDHQVAALGDRYDKLKQSLESNLTNLIKRNTEMEILLSKLIQTCQQVEVNASRQEAKLMEESDQLIEIIQQRRQIIGTKIKEGKVVRLRKLAQQVANCKQCIERSTSLISQAEQSLKENDHARFLQSAKNITERVSMATASSQVLIPEINLNDTFDTFALDFSREKKLLECLDYLTAPNPPVIREELCTASYDTITVHWTSEDEFSVVSYELQYTIFTGQANVVSLCNSADSWMIVPNIKQNHYTVHGLQSGTKYIFIVKAINQAGSRNSEPGKLKTNSQPFKLDPKSAHRKLKVSHDNLTVERDETSSKKSHTPERFTSQGSYGVAGNVFLDSGRHYWEVVISGSTWYAIGIAYKSAPKHEWIGKNTASWVFCRCNNNWAVRHNSKEIPIEPSPHLRRVGILLDYDNGSLSFYDALNSIHLYTFDIAFAQPVCPTLTVWNKCLTILTGLPIPDHLDCLEQLA

Target ORF information:

RefSeq Version	XM_012956770.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis midline 1 (mid1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012956770.2

ORF Insert Sequence:

ATGGAAACAC TGGAGTCTGA ACTGACCTGC CCTATCTGTC TGGAGCTGTT TGAAGATCCA 
CTGCTGCTGC CTTGTGCTCA CAGCCTGTGT TTTAACTGTG CGCATCGCAT CCTTGTCTCC 
CACTGTGCCA ACAACGAATC CGTAGAATCT ATTAACGCCT TCCAGTGCCC CACCTGCCGT 
TATGTCATCA CACTCAGCAA GCGTGGCTTA GAGGGGCTAA AGAGGAACGT GACTTTACAG 
AACATCATTG ACAGATTTCA GAAAGCCTCA GTGAGCGGGC CCAATTCACC TAGTGAAACG 
CGTCGAGAAC GTGCTTACGA TACCACTAAC AGCATGACAT CAAGCGAAAA AGTACTCTGC 
CAGTTCTGTG ATCAGGACCA GGCAAGGGAG GCTGTGAAAA CCTGTGTGAC CTGTGAAGTT 
TCATATTGTG AGGACTGTTT AAAAGCTACT CACCCAAACA AGAAACCATT CACTGGTCAC 
CGACTAATTG AACCCATCCC AGATTCTCAC ATAAGGGGGT TAATGTGCCT GGAACATGAG 
GATGAAAAAG TTAATATGTA TTGTGTGACA GATGACCAGC TAATCTGTGC CTTGTGTAAA 
TTGGTTGGGA GGCATCGAGA TCATCAAGTT GCTGCCCTTG GTGACAGATA CGACAAACTA 
AAGCAAAGCC TGGAAAGCAA CCTTACCAAC CTCATCAAAA GGAACACAGA AATGGAAATT 
CTGTTGTCCA AGCTCATTCA GACCTGCCAG CAGGTGGAGG TTAATGCATC ACGACAAGAA 
GCAAAATTAA TGGAGGAGAG TGACCAACTG ATTGAAATTA TTCAACAGAG AAGACAAATA 
ATTGGAACAA AAATAAAAGA AGGAAAGGTC GTGAGGTTAA GGAAACTGGC CCAGCAGGTT 
GCCAACTGTA AGCAGTGCAT CGAGCGCTCC ACATCCCTGA TCTCACAGGC AGAACAGTCT 
CTAAAGGAAA ACGATCACGC TCGCTTCCTT CAGTCAGCCA AGAATATCAC AGAAAGAGTC 
TCAATGGCAA CAGCATCTTC TCAAGTGCTT ATTCCAGAAA TCAACCTGAA TGACACATTT 
GACACATTTG CATTGGATTT TTCAAGAGAG AAGAAGCTGT TAGAATGTCT TGATTATCTG 
ACAGCTCCCA ATCCCCCTGT AATCAGAGAG GAACTCTGCA CTGCATCCTA CGACACCATT 
ACTGTGCATT GGACATCTGA GGATGAGTTT AGCGTGGTTT CCTATGAGCT GCAGTATACC 
ATCTTCACTG GACAAGCTAA CGTTGTTAGT TTGTGTAACT CAGCCGACAG CTGGATGATT 
GTTCCCAATA TCAAACAGAA CCATTACACA GTTCATGGTC TCCAGAGTGG CACCAAGTAC 
ATCTTCATTG TTAAGGCCAT AAACCAAGCT GGAAGTCGCA ACAGTGAACC TGGCAAGCTA 
AAAACCAACA GTCAACCTTT TAAATTAGAT CCCAAGTCTG CTCACAGGAA ACTTAAGGTA 
TCACATGACA ACCTGACCGT AGAGAGGGAC GAAACCTCTT CAAAAAAGAG CCACACACCA 
GAGCGTTTTA CAAGCCAAGG AAGTTATGGT GTTGCTGGCA ACGTGTTCCT CGACAGTGGC 
CGGCATTATT GGGAAGTGGT TATAAGCGGA AGCACCTGGT ATGCCATTGG CATTGCCTAC 
AAATCTGCAC CCAAGCACGA GTGGATAGGG AAGAATACAG CCTCTTGGGT GTTTTGCCGT 
TGTAACAACA ACTGGGCTGT AAGACACAAC AGTAAAGAGA TTCCTATAGA GCCATCTCCG 
CATCTTCGCC GCGTTGGGAT CTTGCTGGAC TACGACAATG GCTCATTGTC TTTTTATGAT 
GCTTTGAACT CCATTCACCT CTACACTTTT GACATTGCTT TTGCACAGCC CGTGTGCCCA 
ACCCTCACCG TGTGGAATAA GTGCTTAACA ATTTTAACAG GTCTCCCCAT ACCTGATCAC 
TTGGACTGCC TAGAGCAACT AGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mid1 ( XM_012956769.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012812223.1 Xenopus tropicalis midline 1 (mid1), transcript variant X2, mRNA.

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CloneID	OXa02239
Clone ID Related Accession (Same CDS sequence)	XM_012956768.3 , XM_012956769.3 , XM_012956768.2 , XM_012956770.2 , XM_012956770.3 , XM_012956771.3 , XM_012956769.2 , XM_012956771.2 , NM_001006875.1
Accession Version	XM_012956769.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2007bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	E3 ubiquitin-protein ligase Midline-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012956769.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAACAC TGGAGTCTGA ACTGACCTGC CCTATCTGTC TGGAGCTGTT TGAAGATCCA 
CTGCTGCTGC CTTGTGCTCA CAGCCTGTGT TTTAACTGTG CGCATCGCAT CCTTGTCTCC 
CACTGTGCCA ACAACGAATC CGTAGAATCT ATTAACGCCT TCCAGTGCCC CACCTGCCGT 
TATGTCATCA CACTCAGCAA GCGTGGCTTA GAGGGGCTAA AGAGGAACGT GACTTTACAG 
AACATCATTG ACAGATTTCA GAAAGCCTCA GTGAGCGGGC CCAATTCACC TAGTGAAACG 
CGTCGAGAAC GTGCTTACGA TACCACTAAC AGCATGACAT CAAGCGAAAA AGTACTCTGC 
CAGTTCTGTG ATCAGGACCA GGCAAGGGAG GCTGTGAAAA CCTGTGTGAC CTGTGAAGTT 
TCATATTGTG AGGACTGTTT AAAAGCTACT CACCCAAACA AGAAACCATT CACTGGTCAC 
CGACTAATTG AACCCATCCC AGATTCTCAC ATAAGGGGGT TAATGTGCCT GGAACATGAG 
GATGAAAAAG TTAATATGTA TTGTGTGACA GATGACCAGC TAATCTGTGC CTTGTGTAAA 
TTGGTTGGGA GGCATCGAGA TCATCAAGTT GCTGCCCTTG GTGACAGATA CGACAAACTA 
AAGCAAAGCC TGGAAAGCAA CCTTACCAAC CTCATCAAAA GGAACACAGA AATGGAAATT 
CTGTTGTCCA AGCTCATTCA GACCTGCCAG CAGGTGGAGG TTAATGCATC ACGACAAGAA 
GCAAAATTAA TGGAGGAGAG TGACCAACTG ATTGAAATTA TTCAACAGAG AAGACAAATA 
ATTGGAACAA AAATAAAAGA AGGAAAGGTC GTGAGGTTAA GGAAACTGGC CCAGCAGGTT 
GCCAACTGTA AGCAGTGCAT CGAGCGCTCC ACATCCCTGA TCTCACAGGC AGAACAGTCT 
CTAAAGGAAA ACGATCACGC TCGCTTCCTT CAGTCAGCCA AGAATATCAC AGAAAGAGTC 
TCAATGGCAA CAGCATCTTC TCAAGTGCTT ATTCCAGAAA TCAACCTGAA TGACACATTT 
GACACATTTG CATTGGATTT TTCAAGAGAG AAGAAGCTGT TAGAATGTCT TGATTATCTG 
ACAGCTCCCA ATCCCCCTGT AATCAGAGAG GAACTCTGCA CTGCATCCTA CGACACCATT 
ACTGTGCATT GGACATCTGA GGATGAGTTT AGCGTGGTTT CCTATGAGCT GCAGTATACC 
ATCTTCACTG GACAAGCTAA CGTTGTTAGT TTGTGTAACT CAGCCGACAG CTGGATGATT 
GTTCCCAATA TCAAACAGAA CCATTACACA GTTCATGGTC TCCAGAGTGG CACCAAGTAC 
ATCTTCATTG TTAAGGCCAT AAACCAAGCT GGAAGTCGCA ACAGTGAACC TGGCAAGCTA 
AAAACCAACA GTCAACCTTT TAAATTAGAT CCCAAGTCTG CTCACAGGAA ACTTAAGGTA 
TCACATGACA ACCTGACCGT AGAGAGGGAC GAAACCTCTT CAAAAAAGAG CCACACACCA 
GAGCGTTTTA CAAGCCAAGG AAGTTATGGT GTTGCTGGCA ACGTGTTCCT CGACAGTGGC 
CGGCATTATT GGGAAGTGGT TATAAGCGGA AGCACCTGGT ATGCCATTGG CATTGCCTAC 
AAATCTGCAC CCAAGCACGA GTGGATAGGG AAGAATACAG CCTCTTGGGT GTTTTGCCGT 
TGTAACAACA ACTGGGCTGT AAGACACAAC AGTAAAGAGA TTCCTATAGA GCCATCTCCG 
CATCTTCGCC GCGTTGGGAT CTTGCTGGAC TACGACAATG GCTCATTGTC TTTTTATGAT 
GCTTTGAACT CCATTCACCT CTACACTTTT GACATTGCTT TTGCACAGCC CGTGTGCCCA 
ACCCTCACCG TGTGGAATAA GTGCTTAACA ATTTTAACAG GTCTCCCCAT ACCTGATCAC 
TTGGACTGCC TAGAGCAACT AGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012812223.1
CDS	731..2737
Translation	METLESELTCPICLELFEDPLLLPCAHSLCFNCAHRILVSHCANNESVESINAFQCPTCRYVITLSKRGLEGLKRNVTLQNIIDRFQKASVSGPNSPSETRRERAYDTTNSMTSSEKVLCQFCDQDQAREAVKTCVTCEVSYCEDCLKATHPNKKPFTGHRLIEPIPDSHIRGLMCLEHEDEKVNMYCVTDDQLICALCKLVGRHRDHQVAALGDRYDKLKQSLESNLTNLIKRNTEMEILLSKLIQTCQQVEVNASRQEAKLMEESDQLIEIIQQRRQIIGTKIKEGKVVRLRKLAQQVANCKQCIERSTSLISQAEQSLKENDHARFLQSAKNITERVSMATASSQVLIPEINLNDTFDTFALDFSREKKLLECLDYLTAPNPPVIREELCTASYDTITVHWTSEDEFSVVSYELQYTIFTGQANVVSLCNSADSWMIVPNIKQNHYTVHGLQSGTKYIFIVKAINQAGSRNSEPGKLKTNSQPFKLDPKSAHRKLKVSHDNLTVERDETSSKKSHTPERFTSQGSYGVAGNVFLDSGRHYWEVVISGSTWYAIGIAYKSAPKHEWIGKNTASWVFCRCNNNWAVRHNSKEIPIEPSPHLRRVGILLDYDNGSLSFYDALNSIHLYTFDIAFAQPVCPTLTVWNKCLTILTGLPIPDHLDCLEQLA

Target ORF information:

RefSeq Version	XM_012956769.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis midline 1 (mid1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012956769.2

ORF Insert Sequence:

ATGGAAACAC TGGAGTCTGA ACTGACCTGC CCTATCTGTC TGGAGCTGTT TGAAGATCCA 
CTGCTGCTGC CTTGTGCTCA CAGCCTGTGT TTTAACTGTG CGCATCGCAT CCTTGTCTCC 
CACTGTGCCA ACAACGAATC CGTAGAATCT ATTAACGCCT TCCAGTGCCC CACCTGCCGT 
TATGTCATCA CACTCAGCAA GCGTGGCTTA GAGGGGCTAA AGAGGAACGT GACTTTACAG 
AACATCATTG ACAGATTTCA GAAAGCCTCA GTGAGCGGGC CCAATTCACC TAGTGAAACG 
CGTCGAGAAC GTGCTTACGA TACCACTAAC AGCATGACAT CAAGCGAAAA AGTACTCTGC 
CAGTTCTGTG ATCAGGACCA GGCAAGGGAG GCTGTGAAAA CCTGTGTGAC CTGTGAAGTT 
TCATATTGTG AGGACTGTTT AAAAGCTACT CACCCAAACA AGAAACCATT CACTGGTCAC 
CGACTAATTG AACCCATCCC AGATTCTCAC ATAAGGGGGT TAATGTGCCT GGAACATGAG 
GATGAAAAAG TTAATATGTA TTGTGTGACA GATGACCAGC TAATCTGTGC CTTGTGTAAA 
TTGGTTGGGA GGCATCGAGA TCATCAAGTT GCTGCCCTTG GTGACAGATA CGACAAACTA 
AAGCAAAGCC TGGAAAGCAA CCTTACCAAC CTCATCAAAA GGAACACAGA AATGGAAATT 
CTGTTGTCCA AGCTCATTCA GACCTGCCAG CAGGTGGAGG TTAATGCATC ACGACAAGAA 
GCAAAATTAA TGGAGGAGAG TGACCAACTG ATTGAAATTA TTCAACAGAG AAGACAAATA 
ATTGGAACAA AAATAAAAGA AGGAAAGGTC GTGAGGTTAA GGAAACTGGC CCAGCAGGTT 
GCCAACTGTA AGCAGTGCAT CGAGCGCTCC ACATCCCTGA TCTCACAGGC AGAACAGTCT 
CTAAAGGAAA ACGATCACGC TCGCTTCCTT CAGTCAGCCA AGAATATCAC AGAAAGAGTC 
TCAATGGCAA CAGCATCTTC TCAAGTGCTT ATTCCAGAAA TCAACCTGAA TGACACATTT 
GACACATTTG CATTGGATTT TTCAAGAGAG AAGAAGCTGT TAGAATGTCT TGATTATCTG 
ACAGCTCCCA ATCCCCCTGT AATCAGAGAG GAACTCTGCA CTGCATCCTA CGACACCATT 
ACTGTGCATT GGACATCTGA GGATGAGTTT AGCGTGGTTT CCTATGAGCT GCAGTATACC 
ATCTTCACTG GACAAGCTAA CGTTGTTAGT TTGTGTAACT CAGCCGACAG CTGGATGATT 
GTTCCCAATA TCAAACAGAA CCATTACACA GTTCATGGTC TCCAGAGTGG CACCAAGTAC 
ATCTTCATTG TTAAGGCCAT AAACCAAGCT GGAAGTCGCA ACAGTGAACC TGGCAAGCTA 
AAAACCAACA GTCAACCTTT TAAATTAGAT CCCAAGTCTG CTCACAGGAA ACTTAAGGTA 
TCACATGACA ACCTGACCGT AGAGAGGGAC GAAACCTCTT CAAAAAAGAG CCACACACCA 
GAGCGTTTTA CAAGCCAAGG AAGTTATGGT GTTGCTGGCA ACGTGTTCCT CGACAGTGGC 
CGGCATTATT GGGAAGTGGT TATAAGCGGA AGCACCTGGT ATGCCATTGG CATTGCCTAC 
AAATCTGCAC CCAAGCACGA GTGGATAGGG AAGAATACAG CCTCTTGGGT GTTTTGCCGT 
TGTAACAACA ACTGGGCTGT AAGACACAAC AGTAAAGAGA TTCCTATAGA GCCATCTCCG 
CATCTTCGCC GCGTTGGGAT CTTGCTGGAC TACGACAATG GCTCATTGTC TTTTTATGAT 
GCTTTGAACT CCATTCACCT CTACACTTTT GACATTGCTT TTGCACAGCC CGTGTGCCCA 
ACCCTCACCG TGTGGAATAA GTGCTTAACA ATTTTAACAG GTCTCCCCAT ACCTGATCAC 
TTGGACTGCC TAGAGCAACT AGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mid1 ( XM_012956771.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012812225.1 Xenopus tropicalis midline 1 (mid1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OXa02239
Clone ID Related Accession (Same CDS sequence)	XM_012956768.3 , XM_012956769.3 , XM_012956768.2 , XM_012956770.2 , XM_012956770.3 , XM_012956771.3 , XM_012956769.2 , XM_012956771.2 , NM_001006875.1
Accession Version	XM_012956771.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2007bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	E3 ubiquitin-protein ligase Midline-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012956771.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAACAC TGGAGTCTGA ACTGACCTGC CCTATCTGTC TGGAGCTGTT TGAAGATCCA 
CTGCTGCTGC CTTGTGCTCA CAGCCTGTGT TTTAACTGTG CGCATCGCAT CCTTGTCTCC 
CACTGTGCCA ACAACGAATC CGTAGAATCT ATTAACGCCT TCCAGTGCCC CACCTGCCGT 
TATGTCATCA CACTCAGCAA GCGTGGCTTA GAGGGGCTAA AGAGGAACGT GACTTTACAG 
AACATCATTG ACAGATTTCA GAAAGCCTCA GTGAGCGGGC CCAATTCACC TAGTGAAACG 
CGTCGAGAAC GTGCTTACGA TACCACTAAC AGCATGACAT CAAGCGAAAA AGTACTCTGC 
CAGTTCTGTG ATCAGGACCA GGCAAGGGAG GCTGTGAAAA CCTGTGTGAC CTGTGAAGTT 
TCATATTGTG AGGACTGTTT AAAAGCTACT CACCCAAACA AGAAACCATT CACTGGTCAC 
CGACTAATTG AACCCATCCC AGATTCTCAC ATAAGGGGGT TAATGTGCCT GGAACATGAG 
GATGAAAAAG TTAATATGTA TTGTGTGACA GATGACCAGC TAATCTGTGC CTTGTGTAAA 
TTGGTTGGGA GGCATCGAGA TCATCAAGTT GCTGCCCTTG GTGACAGATA CGACAAACTA 
AAGCAAAGCC TGGAAAGCAA CCTTACCAAC CTCATCAAAA GGAACACAGA AATGGAAATT 
CTGTTGTCCA AGCTCATTCA GACCTGCCAG CAGGTGGAGG TTAATGCATC ACGACAAGAA 
GCAAAATTAA TGGAGGAGAG TGACCAACTG ATTGAAATTA TTCAACAGAG AAGACAAATA 
ATTGGAACAA AAATAAAAGA AGGAAAGGTC GTGAGGTTAA GGAAACTGGC CCAGCAGGTT 
GCCAACTGTA AGCAGTGCAT CGAGCGCTCC ACATCCCTGA TCTCACAGGC AGAACAGTCT 
CTAAAGGAAA ACGATCACGC TCGCTTCCTT CAGTCAGCCA AGAATATCAC AGAAAGAGTC 
TCAATGGCAA CAGCATCTTC TCAAGTGCTT ATTCCAGAAA TCAACCTGAA TGACACATTT 
GACACATTTG CATTGGATTT TTCAAGAGAG AAGAAGCTGT TAGAATGTCT TGATTATCTG 
ACAGCTCCCA ATCCCCCTGT AATCAGAGAG GAACTCTGCA CTGCATCCTA CGACACCATT 
ACTGTGCATT GGACATCTGA GGATGAGTTT AGCGTGGTTT CCTATGAGCT GCAGTATACC 
ATCTTCACTG GACAAGCTAA CGTTGTTAGT TTGTGTAACT CAGCCGACAG CTGGATGATT 
GTTCCCAATA TCAAACAGAA CCATTACACA GTTCATGGTC TCCAGAGTGG CACCAAGTAC 
ATCTTCATTG TTAAGGCCAT AAACCAAGCT GGAAGTCGCA ACAGTGAACC TGGCAAGCTA 
AAAACCAACA GTCAACCTTT TAAATTAGAT CCCAAGTCTG CTCACAGGAA ACTTAAGGTA 
TCACATGACA ACCTGACCGT AGAGAGGGAC GAAACCTCTT CAAAAAAGAG CCACACACCA 
GAGCGTTTTA CAAGCCAAGG AAGTTATGGT GTTGCTGGCA ACGTGTTCCT CGACAGTGGC 
CGGCATTATT GGGAAGTGGT TATAAGCGGA AGCACCTGGT ATGCCATTGG CATTGCCTAC 
AAATCTGCAC CCAAGCACGA GTGGATAGGG AAGAATACAG CCTCTTGGGT GTTTTGCCGT 
TGTAACAACA ACTGGGCTGT AAGACACAAC AGTAAAGAGA TTCCTATAGA GCCATCTCCG 
CATCTTCGCC GCGTTGGGAT CTTGCTGGAC TACGACAATG GCTCATTGTC TTTTTATGAT 
GCTTTGAACT CCATTCACCT CTACACTTTT GACATTGCTT TTGCACAGCC CGTGTGCCCA 
ACCCTCACCG TGTGGAATAA GTGCTTAACA ATTTTAACAG GTCTCCCCAT ACCTGATCAC 
TTGGACTGCC TAGAGCAACT AGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012812225.1
CDS	177..2183
Translation	METLESELTCPICLELFEDPLLLPCAHSLCFNCAHRILVSHCANNESVESINAFQCPTCRYVITLSKRGLEGLKRNVTLQNIIDRFQKASVSGPNSPSETRRERAYDTTNSMTSSEKVLCQFCDQDQAREAVKTCVTCEVSYCEDCLKATHPNKKPFTGHRLIEPIPDSHIRGLMCLEHEDEKVNMYCVTDDQLICALCKLVGRHRDHQVAALGDRYDKLKQSLESNLTNLIKRNTEMEILLSKLIQTCQQVEVNASRQEAKLMEESDQLIEIIQQRRQIIGTKIKEGKVVRLRKLAQQVANCKQCIERSTSLISQAEQSLKENDHARFLQSAKNITERVSMATASSQVLIPEINLNDTFDTFALDFSREKKLLECLDYLTAPNPPVIREELCTASYDTITVHWTSEDEFSVVSYELQYTIFTGQANVVSLCNSADSWMIVPNIKQNHYTVHGLQSGTKYIFIVKAINQAGSRNSEPGKLKTNSQPFKLDPKSAHRKLKVSHDNLTVERDETSSKKSHTPERFTSQGSYGVAGNVFLDSGRHYWEVVISGSTWYAIGIAYKSAPKHEWIGKNTASWVFCRCNNNWAVRHNSKEIPIEPSPHLRRVGILLDYDNGSLSFYDALNSIHLYTFDIAFAQPVCPTLTVWNKCLTILTGLPIPDHLDCLEQLA

Target ORF information:

RefSeq Version	XM_012956771.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis midline 1 (mid1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012956771.2

ORF Insert Sequence:

ATGGAAACAC TGGAGTCTGA ACTGACCTGC CCTATCTGTC TGGAGCTGTT TGAAGATCCA 
CTGCTGCTGC CTTGTGCTCA CAGCCTGTGT TTTAACTGTG CGCATCGCAT CCTTGTCTCC 
CACTGTGCCA ACAACGAATC CGTAGAATCT ATTAACGCCT TCCAGTGCCC CACCTGCCGT 
TATGTCATCA CACTCAGCAA GCGTGGCTTA GAGGGGCTAA AGAGGAACGT GACTTTACAG 
AACATCATTG ACAGATTTCA GAAAGCCTCA GTGAGCGGGC CCAATTCACC TAGTGAAACG 
CGTCGAGAAC GTGCTTACGA TACCACTAAC AGCATGACAT CAAGCGAAAA AGTACTCTGC 
CAGTTCTGTG ATCAGGACCA GGCAAGGGAG GCTGTGAAAA CCTGTGTGAC CTGTGAAGTT 
TCATATTGTG AGGACTGTTT AAAAGCTACT CACCCAAACA AGAAACCATT CACTGGTCAC 
CGACTAATTG AACCCATCCC AGATTCTCAC ATAAGGGGGT TAATGTGCCT GGAACATGAG 
GATGAAAAAG TTAATATGTA TTGTGTGACA GATGACCAGC TAATCTGTGC CTTGTGTAAA 
TTGGTTGGGA GGCATCGAGA TCATCAAGTT GCTGCCCTTG GTGACAGATA CGACAAACTA 
AAGCAAAGCC TGGAAAGCAA CCTTACCAAC CTCATCAAAA GGAACACAGA AATGGAAATT 
CTGTTGTCCA AGCTCATTCA GACCTGCCAG CAGGTGGAGG TTAATGCATC ACGACAAGAA 
GCAAAATTAA TGGAGGAGAG TGACCAACTG ATTGAAATTA TTCAACAGAG AAGACAAATA 
ATTGGAACAA AAATAAAAGA AGGAAAGGTC GTGAGGTTAA GGAAACTGGC CCAGCAGGTT 
GCCAACTGTA AGCAGTGCAT CGAGCGCTCC ACATCCCTGA TCTCACAGGC AGAACAGTCT 
CTAAAGGAAA ACGATCACGC TCGCTTCCTT CAGTCAGCCA AGAATATCAC AGAAAGAGTC 
TCAATGGCAA CAGCATCTTC TCAAGTGCTT ATTCCAGAAA TCAACCTGAA TGACACATTT 
GACACATTTG CATTGGATTT TTCAAGAGAG AAGAAGCTGT TAGAATGTCT TGATTATCTG 
ACAGCTCCCA ATCCCCCTGT AATCAGAGAG GAACTCTGCA CTGCATCCTA CGACACCATT 
ACTGTGCATT GGACATCTGA GGATGAGTTT AGCGTGGTTT CCTATGAGCT GCAGTATACC 
ATCTTCACTG GACAAGCTAA CGTTGTTAGT TTGTGTAACT CAGCCGACAG CTGGATGATT 
GTTCCCAATA TCAAACAGAA CCATTACACA GTTCATGGTC TCCAGAGTGG CACCAAGTAC 
ATCTTCATTG TTAAGGCCAT AAACCAAGCT GGAAGTCGCA ACAGTGAACC TGGCAAGCTA 
AAAACCAACA GTCAACCTTT TAAATTAGAT CCCAAGTCTG CTCACAGGAA ACTTAAGGTA 
TCACATGACA ACCTGACCGT AGAGAGGGAC GAAACCTCTT CAAAAAAGAG CCACACACCA 
GAGCGTTTTA CAAGCCAAGG AAGTTATGGT GTTGCTGGCA ACGTGTTCCT CGACAGTGGC 
CGGCATTATT GGGAAGTGGT TATAAGCGGA AGCACCTGGT ATGCCATTGG CATTGCCTAC 
AAATCTGCAC CCAAGCACGA GTGGATAGGG AAGAATACAG CCTCTTGGGT GTTTTGCCGT 
TGTAACAACA ACTGGGCTGT AAGACACAAC AGTAAAGAGA TTCCTATAGA GCCATCTCCG 
CATCTTCGCC GCGTTGGGAT CTTGCTGGAC TACGACAATG GCTCATTGTC TTTTTATGAT 
GCTTTGAACT CCATTCACCT CTACACTTTT GACATTGCTT TTGCACAGCC CGTGTGCCCA 
ACCCTCACCG TGTGGAATAA GTGCTTAACA ATTTTAACAG GTCTCCCCAT ACCTGATCAC 
TTGGACTGCC TAGAGCAACT AGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mid1 ( XM_012956768.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012812222.1 Xenopus tropicalis midline 1 (mid1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa02239
Clone ID Related Accession (Same CDS sequence)	XM_012956768.3 , XM_012956769.3 , XM_012956768.2 , XM_012956770.2 , XM_012956770.3 , XM_012956771.3 , XM_012956769.2 , XM_012956771.2 , NM_001006875.1
Accession Version	XM_012956768.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2007bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	E3 ubiquitin-protein ligase Midline-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012956768.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAACAC TGGAGTCTGA ACTGACCTGC CCTATCTGTC TGGAGCTGTT TGAAGATCCA 
CTGCTGCTGC CTTGTGCTCA CAGCCTGTGT TTTAACTGTG CGCATCGCAT CCTTGTCTCC 
CACTGTGCCA ACAACGAATC CGTAGAATCT ATTAACGCCT TCCAGTGCCC CACCTGCCGT 
TATGTCATCA CACTCAGCAA GCGTGGCTTA GAGGGGCTAA AGAGGAACGT GACTTTACAG 
AACATCATTG ACAGATTTCA GAAAGCCTCA GTGAGCGGGC CCAATTCACC TAGTGAAACG 
CGTCGAGAAC GTGCTTACGA TACCACTAAC AGCATGACAT CAAGCGAAAA AGTACTCTGC 
CAGTTCTGTG ATCAGGACCA GGCAAGGGAG GCTGTGAAAA CCTGTGTGAC CTGTGAAGTT 
TCATATTGTG AGGACTGTTT AAAAGCTACT CACCCAAACA AGAAACCATT CACTGGTCAC 
CGACTAATTG AACCCATCCC AGATTCTCAC ATAAGGGGGT TAATGTGCCT GGAACATGAG 
GATGAAAAAG TTAATATGTA TTGTGTGACA GATGACCAGC TAATCTGTGC CTTGTGTAAA 
TTGGTTGGGA GGCATCGAGA TCATCAAGTT GCTGCCCTTG GTGACAGATA CGACAAACTA 
AAGCAAAGCC TGGAAAGCAA CCTTACCAAC CTCATCAAAA GGAACACAGA AATGGAAATT 
CTGTTGTCCA AGCTCATTCA GACCTGCCAG CAGGTGGAGG TTAATGCATC ACGACAAGAA 
GCAAAATTAA TGGAGGAGAG TGACCAACTG ATTGAAATTA TTCAACAGAG AAGACAAATA 
ATTGGAACAA AAATAAAAGA AGGAAAGGTC GTGAGGTTAA GGAAACTGGC CCAGCAGGTT 
GCCAACTGTA AGCAGTGCAT CGAGCGCTCC ACATCCCTGA TCTCACAGGC AGAACAGTCT 
CTAAAGGAAA ACGATCACGC TCGCTTCCTT CAGTCAGCCA AGAATATCAC AGAAAGAGTC 
TCAATGGCAA CAGCATCTTC TCAAGTGCTT ATTCCAGAAA TCAACCTGAA TGACACATTT 
GACACATTTG CATTGGATTT TTCAAGAGAG AAGAAGCTGT TAGAATGTCT TGATTATCTG 
ACAGCTCCCA ATCCCCCTGT AATCAGAGAG GAACTCTGCA CTGCATCCTA CGACACCATT 
ACTGTGCATT GGACATCTGA GGATGAGTTT AGCGTGGTTT CCTATGAGCT GCAGTATACC 
ATCTTCACTG GACAAGCTAA CGTTGTTAGT TTGTGTAACT CAGCCGACAG CTGGATGATT 
GTTCCCAATA TCAAACAGAA CCATTACACA GTTCATGGTC TCCAGAGTGG CACCAAGTAC 
ATCTTCATTG TTAAGGCCAT AAACCAAGCT GGAAGTCGCA ACAGTGAACC TGGCAAGCTA 
AAAACCAACA GTCAACCTTT TAAATTAGAT CCCAAGTCTG CTCACAGGAA ACTTAAGGTA 
TCACATGACA ACCTGACCGT AGAGAGGGAC GAAACCTCTT CAAAAAAGAG CCACACACCA 
GAGCGTTTTA CAAGCCAAGG AAGTTATGGT GTTGCTGGCA ACGTGTTCCT CGACAGTGGC 
CGGCATTATT GGGAAGTGGT TATAAGCGGA AGCACCTGGT ATGCCATTGG CATTGCCTAC 
AAATCTGCAC CCAAGCACGA GTGGATAGGG AAGAATACAG CCTCTTGGGT GTTTTGCCGT 
TGTAACAACA ACTGGGCTGT AAGACACAAC AGTAAAGAGA TTCCTATAGA GCCATCTCCG 
CATCTTCGCC GCGTTGGGAT CTTGCTGGAC TACGACAATG GCTCATTGTC TTTTTATGAT 
GCTTTGAACT CCATTCACCT CTACACTTTT GACATTGCTT TTGCACAGCC CGTGTGCCCA 
ACCCTCACCG TGTGGAATAA GTGCTTAACA ATTTTAACAG GTCTCCCCAT ACCTGATCAC 
TTGGACTGCC TAGAGCAACT AGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012812222.1
CDS	338..2344
Translation	METLESELTCPICLELFEDPLLLPCAHSLCFNCAHRILVSHCANNESVESINAFQCPTCRYVITLSKRGLEGLKRNVTLQNIIDRFQKASVSGPNSPSETRRERAYDTTNSMTSSEKVLCQFCDQDQAREAVKTCVTCEVSYCEDCLKATHPNKKPFTGHRLIEPIPDSHIRGLMCLEHEDEKVNMYCVTDDQLICALCKLVGRHRDHQVAALGDRYDKLKQSLESNLTNLIKRNTEMEILLSKLIQTCQQVEVNASRQEAKLMEESDQLIEIIQQRRQIIGTKIKEGKVVRLRKLAQQVANCKQCIERSTSLISQAEQSLKENDHARFLQSAKNITERVSMATASSQVLIPEINLNDTFDTFALDFSREKKLLECLDYLTAPNPPVIREELCTASYDTITVHWTSEDEFSVVSYELQYTIFTGQANVVSLCNSADSWMIVPNIKQNHYTVHGLQSGTKYIFIVKAINQAGSRNSEPGKLKTNSQPFKLDPKSAHRKLKVSHDNLTVERDETSSKKSHTPERFTSQGSYGVAGNVFLDSGRHYWEVVISGSTWYAIGIAYKSAPKHEWIGKNTASWVFCRCNNNWAVRHNSKEIPIEPSPHLRRVGILLDYDNGSLSFYDALNSIHLYTFDIAFAQPVCPTLTVWNKCLTILTGLPIPDHLDCLEQLA

Target ORF information:

RefSeq Version	XM_012956768.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis midline 1 (mid1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012956768.3

ORF Insert Sequence:

ATGGAAACAC TGGAGTCTGA ACTGACCTGC CCTATCTGTC TGGAGCTGTT TGAAGATCCA 
CTGCTGCTGC CTTGTGCTCA CAGCCTGTGT TTTAACTGTG CGCATCGCAT CCTTGTCTCC 
CACTGTGCCA ACAACGAATC CGTAGAATCT ATTAACGCCT TCCAGTGCCC CACCTGCCGT 
TATGTCATCA CACTCAGCAA GCGTGGCTTA GAGGGGCTAA AGAGGAACGT GACTTTACAG 
AACATCATTG ACAGATTTCA GAAAGCCTCA GTGAGCGGGC CCAATTCACC TAGTGAAACG 
CGTCGAGAAC GTGCTTACGA TACCACTAAC AGCATGACAT CAAGCGAAAA AGTACTCTGC 
CAGTTCTGTG ATCAGGACCA GGCAAGGGAG GCTGTGAAAA CCTGTGTGAC CTGTGAAGTT 
TCATATTGTG AGGACTGTTT AAAAGCTACT CACCCAAACA AGAAACCATT CACTGGTCAC 
CGACTAATTG AACCCATCCC AGATTCTCAC ATAAGGGGGT TAATGTGCCT GGAACATGAG 
GATGAAAAAG TTAATATGTA TTGTGTGACA GATGACCAGC TAATCTGTGC CTTGTGTAAA 
TTGGTTGGGA GGCATCGAGA TCATCAAGTT GCTGCCCTTG GTGACAGATA CGACAAACTA 
AAGCAAAGCC TGGAAAGCAA CCTTACCAAC CTCATCAAAA GGAACACAGA AATGGAAATT 
CTGTTGTCCA AGCTCATTCA GACCTGCCAG CAGGTGGAGG TTAATGCATC ACGACAAGAA 
GCAAAATTAA TGGAGGAGAG TGACCAACTG ATTGAAATTA TTCAACAGAG AAGACAAATA 
ATTGGAACAA AAATAAAAGA AGGAAAGGTC GTGAGGTTAA GGAAACTGGC CCAGCAGGTT 
GCCAACTGTA AGCAGTGCAT CGAGCGCTCC ACATCCCTGA TCTCACAGGC AGAACAGTCT 
CTAAAGGAAA ACGATCACGC TCGCTTCCTT CAGTCAGCCA AGAATATCAC AGAAAGAGTC 
TCAATGGCAA CAGCATCTTC TCAAGTGCTT ATTCCAGAAA TCAACCTGAA TGACACATTT 
GACACATTTG CATTGGATTT TTCAAGAGAG AAGAAGCTGT TAGAATGTCT TGATTATCTG 
ACAGCTCCCA ATCCCCCTGT AATCAGAGAG GAACTCTGCA CTGCATCCTA CGACACCATT 
ACTGTGCATT GGACATCTGA GGATGAGTTT AGCGTGGTTT CCTATGAGCT GCAGTATACC 
ATCTTCACTG GACAAGCTAA CGTTGTTAGT TTGTGTAACT CAGCCGACAG CTGGATGATT 
GTTCCCAATA TCAAACAGAA CCATTACACA GTTCATGGTC TCCAGAGTGG CACCAAGTAC 
ATCTTCATTG TTAAGGCCAT AAACCAAGCT GGAAGTCGCA ACAGTGAACC TGGCAAGCTA 
AAAACCAACA GTCAACCTTT TAAATTAGAT CCCAAGTCTG CTCACAGGAA ACTTAAGGTA 
TCACATGACA ACCTGACCGT AGAGAGGGAC GAAACCTCTT CAAAAAAGAG CCACACACCA 
GAGCGTTTTA CAAGCCAAGG AAGTTATGGT GTTGCTGGCA ACGTGTTCCT CGACAGTGGC 
CGGCATTATT GGGAAGTGGT TATAAGCGGA AGCACCTGGT ATGCCATTGG CATTGCCTAC 
AAATCTGCAC CCAAGCACGA GTGGATAGGG AAGAATACAG CCTCTTGGGT GTTTTGCCGT 
TGTAACAACA ACTGGGCTGT AAGACACAAC AGTAAAGAGA TTCCTATAGA GCCATCTCCG 
CATCTTCGCC GCGTTGGGAT CTTGCTGGAC TACGACAATG GCTCATTGTC TTTTTATGAT 
GCTTTGAACT CCATTCACCT CTACACTTTT GACATTGCTT TTGCACAGCC CGTGTGCCCA 
ACCCTCACCG TGTGGAATAA GTGCTTAACA ATTTTAACAG GTCTCCCCAT ACCTGATCAC 
TTGGACTGCC TAGAGCAACT AGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mid1 ( XM_012956769.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012812223.1 Xenopus tropicalis midline 1 (mid1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa02239
Clone ID Related Accession (Same CDS sequence)	XM_012956768.3 , XM_012956769.3 , XM_012956768.2 , XM_012956770.2 , XM_012956770.3 , XM_012956771.3 , XM_012956769.2 , XM_012956771.2 , NM_001006875.1
Accession Version	XM_012956769.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2007bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	E3 ubiquitin-protein ligase Midline-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012956769.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAACAC TGGAGTCTGA ACTGACCTGC CCTATCTGTC TGGAGCTGTT TGAAGATCCA 
CTGCTGCTGC CTTGTGCTCA CAGCCTGTGT TTTAACTGTG CGCATCGCAT CCTTGTCTCC 
CACTGTGCCA ACAACGAATC CGTAGAATCT ATTAACGCCT TCCAGTGCCC CACCTGCCGT 
TATGTCATCA CACTCAGCAA GCGTGGCTTA GAGGGGCTAA AGAGGAACGT GACTTTACAG 
AACATCATTG ACAGATTTCA GAAAGCCTCA GTGAGCGGGC CCAATTCACC TAGTGAAACG 
CGTCGAGAAC GTGCTTACGA TACCACTAAC AGCATGACAT CAAGCGAAAA AGTACTCTGC 
CAGTTCTGTG ATCAGGACCA GGCAAGGGAG GCTGTGAAAA CCTGTGTGAC CTGTGAAGTT 
TCATATTGTG AGGACTGTTT AAAAGCTACT CACCCAAACA AGAAACCATT CACTGGTCAC 
CGACTAATTG AACCCATCCC AGATTCTCAC ATAAGGGGGT TAATGTGCCT GGAACATGAG 
GATGAAAAAG TTAATATGTA TTGTGTGACA GATGACCAGC TAATCTGTGC CTTGTGTAAA 
TTGGTTGGGA GGCATCGAGA TCATCAAGTT GCTGCCCTTG GTGACAGATA CGACAAACTA 
AAGCAAAGCC TGGAAAGCAA CCTTACCAAC CTCATCAAAA GGAACACAGA AATGGAAATT 
CTGTTGTCCA AGCTCATTCA GACCTGCCAG CAGGTGGAGG TTAATGCATC ACGACAAGAA 
GCAAAATTAA TGGAGGAGAG TGACCAACTG ATTGAAATTA TTCAACAGAG AAGACAAATA 
ATTGGAACAA AAATAAAAGA AGGAAAGGTC GTGAGGTTAA GGAAACTGGC CCAGCAGGTT 
GCCAACTGTA AGCAGTGCAT CGAGCGCTCC ACATCCCTGA TCTCACAGGC AGAACAGTCT 
CTAAAGGAAA ACGATCACGC TCGCTTCCTT CAGTCAGCCA AGAATATCAC AGAAAGAGTC 
TCAATGGCAA CAGCATCTTC TCAAGTGCTT ATTCCAGAAA TCAACCTGAA TGACACATTT 
GACACATTTG CATTGGATTT TTCAAGAGAG AAGAAGCTGT TAGAATGTCT TGATTATCTG 
ACAGCTCCCA ATCCCCCTGT AATCAGAGAG GAACTCTGCA CTGCATCCTA CGACACCATT 
ACTGTGCATT GGACATCTGA GGATGAGTTT AGCGTGGTTT CCTATGAGCT GCAGTATACC 
ATCTTCACTG GACAAGCTAA CGTTGTTAGT TTGTGTAACT CAGCCGACAG CTGGATGATT 
GTTCCCAATA TCAAACAGAA CCATTACACA GTTCATGGTC TCCAGAGTGG CACCAAGTAC 
ATCTTCATTG TTAAGGCCAT AAACCAAGCT GGAAGTCGCA ACAGTGAACC TGGCAAGCTA 
AAAACCAACA GTCAACCTTT TAAATTAGAT CCCAAGTCTG CTCACAGGAA ACTTAAGGTA 
TCACATGACA ACCTGACCGT AGAGAGGGAC GAAACCTCTT CAAAAAAGAG CCACACACCA 
GAGCGTTTTA CAAGCCAAGG AAGTTATGGT GTTGCTGGCA ACGTGTTCCT CGACAGTGGC 
CGGCATTATT GGGAAGTGGT TATAAGCGGA AGCACCTGGT ATGCCATTGG CATTGCCTAC 
AAATCTGCAC CCAAGCACGA GTGGATAGGG AAGAATACAG CCTCTTGGGT GTTTTGCCGT 
TGTAACAACA ACTGGGCTGT AAGACACAAC AGTAAAGAGA TTCCTATAGA GCCATCTCCG 
CATCTTCGCC GCGTTGGGAT CTTGCTGGAC TACGACAATG GCTCATTGTC TTTTTATGAT 
GCTTTGAACT CCATTCACCT CTACACTTTT GACATTGCTT TTGCACAGCC CGTGTGCCCA 
ACCCTCACCG TGTGGAATAA GTGCTTAACA ATTTTAACAG GTCTCCCCAT ACCTGATCAC 
TTGGACTGCC TAGAGCAACT AGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012812223.1
CDS	706..2712
Translation	METLESELTCPICLELFEDPLLLPCAHSLCFNCAHRILVSHCANNESVESINAFQCPTCRYVITLSKRGLEGLKRNVTLQNIIDRFQKASVSGPNSPSETRRERAYDTTNSMTSSEKVLCQFCDQDQAREAVKTCVTCEVSYCEDCLKATHPNKKPFTGHRLIEPIPDSHIRGLMCLEHEDEKVNMYCVTDDQLICALCKLVGRHRDHQVAALGDRYDKLKQSLESNLTNLIKRNTEMEILLSKLIQTCQQVEVNASRQEAKLMEESDQLIEIIQQRRQIIGTKIKEGKVVRLRKLAQQVANCKQCIERSTSLISQAEQSLKENDHARFLQSAKNITERVSMATASSQVLIPEINLNDTFDTFALDFSREKKLLECLDYLTAPNPPVIREELCTASYDTITVHWTSEDEFSVVSYELQYTIFTGQANVVSLCNSADSWMIVPNIKQNHYTVHGLQSGTKYIFIVKAINQAGSRNSEPGKLKTNSQPFKLDPKSAHRKLKVSHDNLTVERDETSSKKSHTPERFTSQGSYGVAGNVFLDSGRHYWEVVISGSTWYAIGIAYKSAPKHEWIGKNTASWVFCRCNNNWAVRHNSKEIPIEPSPHLRRVGILLDYDNGSLSFYDALNSIHLYTFDIAFAQPVCPTLTVWNKCLTILTGLPIPDHLDCLEQLA

Target ORF information:

RefSeq Version	XM_012956769.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis midline 1 (mid1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012956769.3

ORF Insert Sequence:

ATGGAAACAC TGGAGTCTGA ACTGACCTGC CCTATCTGTC TGGAGCTGTT TGAAGATCCA 
CTGCTGCTGC CTTGTGCTCA CAGCCTGTGT TTTAACTGTG CGCATCGCAT CCTTGTCTCC 
CACTGTGCCA ACAACGAATC CGTAGAATCT ATTAACGCCT TCCAGTGCCC CACCTGCCGT 
TATGTCATCA CACTCAGCAA GCGTGGCTTA GAGGGGCTAA AGAGGAACGT GACTTTACAG 
AACATCATTG ACAGATTTCA GAAAGCCTCA GTGAGCGGGC CCAATTCACC TAGTGAAACG 
CGTCGAGAAC GTGCTTACGA TACCACTAAC AGCATGACAT CAAGCGAAAA AGTACTCTGC 
CAGTTCTGTG ATCAGGACCA GGCAAGGGAG GCTGTGAAAA CCTGTGTGAC CTGTGAAGTT 
TCATATTGTG AGGACTGTTT AAAAGCTACT CACCCAAACA AGAAACCATT CACTGGTCAC 
CGACTAATTG AACCCATCCC AGATTCTCAC ATAAGGGGGT TAATGTGCCT GGAACATGAG 
GATGAAAAAG TTAATATGTA TTGTGTGACA GATGACCAGC TAATCTGTGC CTTGTGTAAA 
TTGGTTGGGA GGCATCGAGA TCATCAAGTT GCTGCCCTTG GTGACAGATA CGACAAACTA 
AAGCAAAGCC TGGAAAGCAA CCTTACCAAC CTCATCAAAA GGAACACAGA AATGGAAATT 
CTGTTGTCCA AGCTCATTCA GACCTGCCAG CAGGTGGAGG TTAATGCATC ACGACAAGAA 
GCAAAATTAA TGGAGGAGAG TGACCAACTG ATTGAAATTA TTCAACAGAG AAGACAAATA 
ATTGGAACAA AAATAAAAGA AGGAAAGGTC GTGAGGTTAA GGAAACTGGC CCAGCAGGTT 
GCCAACTGTA AGCAGTGCAT CGAGCGCTCC ACATCCCTGA TCTCACAGGC AGAACAGTCT 
CTAAAGGAAA ACGATCACGC TCGCTTCCTT CAGTCAGCCA AGAATATCAC AGAAAGAGTC 
TCAATGGCAA CAGCATCTTC TCAAGTGCTT ATTCCAGAAA TCAACCTGAA TGACACATTT 
GACACATTTG CATTGGATTT TTCAAGAGAG AAGAAGCTGT TAGAATGTCT TGATTATCTG 
ACAGCTCCCA ATCCCCCTGT AATCAGAGAG GAACTCTGCA CTGCATCCTA CGACACCATT 
ACTGTGCATT GGACATCTGA GGATGAGTTT AGCGTGGTTT CCTATGAGCT GCAGTATACC 
ATCTTCACTG GACAAGCTAA CGTTGTTAGT TTGTGTAACT CAGCCGACAG CTGGATGATT 
GTTCCCAATA TCAAACAGAA CCATTACACA GTTCATGGTC TCCAGAGTGG CACCAAGTAC 
ATCTTCATTG TTAAGGCCAT AAACCAAGCT GGAAGTCGCA ACAGTGAACC TGGCAAGCTA 
AAAACCAACA GTCAACCTTT TAAATTAGAT CCCAAGTCTG CTCACAGGAA ACTTAAGGTA 
TCACATGACA ACCTGACCGT AGAGAGGGAC GAAACCTCTT CAAAAAAGAG CCACACACCA 
GAGCGTTTTA CAAGCCAAGG AAGTTATGGT GTTGCTGGCA ACGTGTTCCT CGACAGTGGC 
CGGCATTATT GGGAAGTGGT TATAAGCGGA AGCACCTGGT ATGCCATTGG CATTGCCTAC 
AAATCTGCAC CCAAGCACGA GTGGATAGGG AAGAATACAG CCTCTTGGGT GTTTTGCCGT 
TGTAACAACA ACTGGGCTGT AAGACACAAC AGTAAAGAGA TTCCTATAGA GCCATCTCCG 
CATCTTCGCC GCGTTGGGAT CTTGCTGGAC TACGACAATG GCTCATTGTC TTTTTATGAT 
GCTTTGAACT CCATTCACCT CTACACTTTT GACATTGCTT TTGCACAGCC CGTGTGCCCA 
ACCCTCACCG TGTGGAATAA GTGCTTAACA ATTTTAACAG GTCTCCCCAT ACCTGATCAC 
TTGGACTGCC TAGAGCAACT AGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mid1 ( XM_012956770.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012812224.1 Xenopus tropicalis midline 1 (mid1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OXa02239
Clone ID Related Accession (Same CDS sequence)	XM_012956768.3 , XM_012956769.3 , XM_012956768.2 , XM_012956770.2 , XM_012956770.3 , XM_012956771.3 , XM_012956769.2 , XM_012956771.2 , NM_001006875.1
Accession Version	XM_012956770.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2007bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	E3 ubiquitin-protein ligase Midline-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012956770.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAACAC TGGAGTCTGA ACTGACCTGC CCTATCTGTC TGGAGCTGTT TGAAGATCCA 
CTGCTGCTGC CTTGTGCTCA CAGCCTGTGT TTTAACTGTG CGCATCGCAT CCTTGTCTCC 
CACTGTGCCA ACAACGAATC CGTAGAATCT ATTAACGCCT TCCAGTGCCC CACCTGCCGT 
TATGTCATCA CACTCAGCAA GCGTGGCTTA GAGGGGCTAA AGAGGAACGT GACTTTACAG 
AACATCATTG ACAGATTTCA GAAAGCCTCA GTGAGCGGGC CCAATTCACC TAGTGAAACG 
CGTCGAGAAC GTGCTTACGA TACCACTAAC AGCATGACAT CAAGCGAAAA AGTACTCTGC 
CAGTTCTGTG ATCAGGACCA GGCAAGGGAG GCTGTGAAAA CCTGTGTGAC CTGTGAAGTT 
TCATATTGTG AGGACTGTTT AAAAGCTACT CACCCAAACA AGAAACCATT CACTGGTCAC 
CGACTAATTG AACCCATCCC AGATTCTCAC ATAAGGGGGT TAATGTGCCT GGAACATGAG 
GATGAAAAAG TTAATATGTA TTGTGTGACA GATGACCAGC TAATCTGTGC CTTGTGTAAA 
TTGGTTGGGA GGCATCGAGA TCATCAAGTT GCTGCCCTTG GTGACAGATA CGACAAACTA 
AAGCAAAGCC TGGAAAGCAA CCTTACCAAC CTCATCAAAA GGAACACAGA AATGGAAATT 
CTGTTGTCCA AGCTCATTCA GACCTGCCAG CAGGTGGAGG TTAATGCATC ACGACAAGAA 
GCAAAATTAA TGGAGGAGAG TGACCAACTG ATTGAAATTA TTCAACAGAG AAGACAAATA 
ATTGGAACAA AAATAAAAGA AGGAAAGGTC GTGAGGTTAA GGAAACTGGC CCAGCAGGTT 
GCCAACTGTA AGCAGTGCAT CGAGCGCTCC ACATCCCTGA TCTCACAGGC AGAACAGTCT 
CTAAAGGAAA ACGATCACGC TCGCTTCCTT CAGTCAGCCA AGAATATCAC AGAAAGAGTC 
TCAATGGCAA CAGCATCTTC TCAAGTGCTT ATTCCAGAAA TCAACCTGAA TGACACATTT 
GACACATTTG CATTGGATTT TTCAAGAGAG AAGAAGCTGT TAGAATGTCT TGATTATCTG 
ACAGCTCCCA ATCCCCCTGT AATCAGAGAG GAACTCTGCA CTGCATCCTA CGACACCATT 
ACTGTGCATT GGACATCTGA GGATGAGTTT AGCGTGGTTT CCTATGAGCT GCAGTATACC 
ATCTTCACTG GACAAGCTAA CGTTGTTAGT TTGTGTAACT CAGCCGACAG CTGGATGATT 
GTTCCCAATA TCAAACAGAA CCATTACACA GTTCATGGTC TCCAGAGTGG CACCAAGTAC 
ATCTTCATTG TTAAGGCCAT AAACCAAGCT GGAAGTCGCA ACAGTGAACC TGGCAAGCTA 
AAAACCAACA GTCAACCTTT TAAATTAGAT CCCAAGTCTG CTCACAGGAA ACTTAAGGTA 
TCACATGACA ACCTGACCGT AGAGAGGGAC GAAACCTCTT CAAAAAAGAG CCACACACCA 
GAGCGTTTTA CAAGCCAAGG AAGTTATGGT GTTGCTGGCA ACGTGTTCCT CGACAGTGGC 
CGGCATTATT GGGAAGTGGT TATAAGCGGA AGCACCTGGT ATGCCATTGG CATTGCCTAC 
AAATCTGCAC CCAAGCACGA GTGGATAGGG AAGAATACAG CCTCTTGGGT GTTTTGCCGT 
TGTAACAACA ACTGGGCTGT AAGACACAAC AGTAAAGAGA TTCCTATAGA GCCATCTCCG 
CATCTTCGCC GCGTTGGGAT CTTGCTGGAC TACGACAATG GCTCATTGTC TTTTTATGAT 
GCTTTGAACT CCATTCACCT CTACACTTTT GACATTGCTT TTGCACAGCC CGTGTGCCCA 
ACCCTCACCG TGTGGAATAA GTGCTTAACA ATTTTAACAG GTCTCCCCAT ACCTGATCAC 
TTGGACTGCC TAGAGCAACT AGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012812224.1
CDS	164..2170
Translation	METLESELTCPICLELFEDPLLLPCAHSLCFNCAHRILVSHCANNESVESINAFQCPTCRYVITLSKRGLEGLKRNVTLQNIIDRFQKASVSGPNSPSETRRERAYDTTNSMTSSEKVLCQFCDQDQAREAVKTCVTCEVSYCEDCLKATHPNKKPFTGHRLIEPIPDSHIRGLMCLEHEDEKVNMYCVTDDQLICALCKLVGRHRDHQVAALGDRYDKLKQSLESNLTNLIKRNTEMEILLSKLIQTCQQVEVNASRQEAKLMEESDQLIEIIQQRRQIIGTKIKEGKVVRLRKLAQQVANCKQCIERSTSLISQAEQSLKENDHARFLQSAKNITERVSMATASSQVLIPEINLNDTFDTFALDFSREKKLLECLDYLTAPNPPVIREELCTASYDTITVHWTSEDEFSVVSYELQYTIFTGQANVVSLCNSADSWMIVPNIKQNHYTVHGLQSGTKYIFIVKAINQAGSRNSEPGKLKTNSQPFKLDPKSAHRKLKVSHDNLTVERDETSSKKSHTPERFTSQGSYGVAGNVFLDSGRHYWEVVISGSTWYAIGIAYKSAPKHEWIGKNTASWVFCRCNNNWAVRHNSKEIPIEPSPHLRRVGILLDYDNGSLSFYDALNSIHLYTFDIAFAQPVCPTLTVWNKCLTILTGLPIPDHLDCLEQLA

Target ORF information:

RefSeq Version	XM_012956770.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis midline 1 (mid1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012956770.3

ORF Insert Sequence:

ATGGAAACAC TGGAGTCTGA ACTGACCTGC CCTATCTGTC TGGAGCTGTT TGAAGATCCA 
CTGCTGCTGC CTTGTGCTCA CAGCCTGTGT TTTAACTGTG CGCATCGCAT CCTTGTCTCC 
CACTGTGCCA ACAACGAATC CGTAGAATCT ATTAACGCCT TCCAGTGCCC CACCTGCCGT 
TATGTCATCA CACTCAGCAA GCGTGGCTTA GAGGGGCTAA AGAGGAACGT GACTTTACAG 
AACATCATTG ACAGATTTCA GAAAGCCTCA GTGAGCGGGC CCAATTCACC TAGTGAAACG 
CGTCGAGAAC GTGCTTACGA TACCACTAAC AGCATGACAT CAAGCGAAAA AGTACTCTGC 
CAGTTCTGTG ATCAGGACCA GGCAAGGGAG GCTGTGAAAA CCTGTGTGAC CTGTGAAGTT 
TCATATTGTG AGGACTGTTT AAAAGCTACT CACCCAAACA AGAAACCATT CACTGGTCAC 
CGACTAATTG AACCCATCCC AGATTCTCAC ATAAGGGGGT TAATGTGCCT GGAACATGAG 
GATGAAAAAG TTAATATGTA TTGTGTGACA GATGACCAGC TAATCTGTGC CTTGTGTAAA 
TTGGTTGGGA GGCATCGAGA TCATCAAGTT GCTGCCCTTG GTGACAGATA CGACAAACTA 
AAGCAAAGCC TGGAAAGCAA CCTTACCAAC CTCATCAAAA GGAACACAGA AATGGAAATT 
CTGTTGTCCA AGCTCATTCA GACCTGCCAG CAGGTGGAGG TTAATGCATC ACGACAAGAA 
GCAAAATTAA TGGAGGAGAG TGACCAACTG ATTGAAATTA TTCAACAGAG AAGACAAATA 
ATTGGAACAA AAATAAAAGA AGGAAAGGTC GTGAGGTTAA GGAAACTGGC CCAGCAGGTT 
GCCAACTGTA AGCAGTGCAT CGAGCGCTCC ACATCCCTGA TCTCACAGGC AGAACAGTCT 
CTAAAGGAAA ACGATCACGC TCGCTTCCTT CAGTCAGCCA AGAATATCAC AGAAAGAGTC 
TCAATGGCAA CAGCATCTTC TCAAGTGCTT ATTCCAGAAA TCAACCTGAA TGACACATTT 
GACACATTTG CATTGGATTT TTCAAGAGAG AAGAAGCTGT TAGAATGTCT TGATTATCTG 
ACAGCTCCCA ATCCCCCTGT AATCAGAGAG GAACTCTGCA CTGCATCCTA CGACACCATT 
ACTGTGCATT GGACATCTGA GGATGAGTTT AGCGTGGTTT CCTATGAGCT GCAGTATACC 
ATCTTCACTG GACAAGCTAA CGTTGTTAGT TTGTGTAACT CAGCCGACAG CTGGATGATT 
GTTCCCAATA TCAAACAGAA CCATTACACA GTTCATGGTC TCCAGAGTGG CACCAAGTAC 
ATCTTCATTG TTAAGGCCAT AAACCAAGCT GGAAGTCGCA ACAGTGAACC TGGCAAGCTA 
AAAACCAACA GTCAACCTTT TAAATTAGAT CCCAAGTCTG CTCACAGGAA ACTTAAGGTA 
TCACATGACA ACCTGACCGT AGAGAGGGAC GAAACCTCTT CAAAAAAGAG CCACACACCA 
GAGCGTTTTA CAAGCCAAGG AAGTTATGGT GTTGCTGGCA ACGTGTTCCT CGACAGTGGC 
CGGCATTATT GGGAAGTGGT TATAAGCGGA AGCACCTGGT ATGCCATTGG CATTGCCTAC 
AAATCTGCAC CCAAGCACGA GTGGATAGGG AAGAATACAG CCTCTTGGGT GTTTTGCCGT 
TGTAACAACA ACTGGGCTGT AAGACACAAC AGTAAAGAGA TTCCTATAGA GCCATCTCCG 
CATCTTCGCC GCGTTGGGAT CTTGCTGGAC TACGACAATG GCTCATTGTC TTTTTATGAT 
GCTTTGAACT CCATTCACCT CTACACTTTT GACATTGCTT TTGCACAGCC CGTGTGCCCA 
ACCCTCACCG TGTGGAATAA GTGCTTAACA ATTTTAACAG GTCTCCCCAT ACCTGATCAC 
TTGGACTGCC TAGAGCAACT AGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mid1 ( XM_012956771.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012812225.1 Xenopus tropicalis midline 1 (mid1), transcript variant X4, mRNA.

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CloneID	OXa02239
Clone ID Related Accession (Same CDS sequence)	XM_012956768.3 , XM_012956769.3 , XM_012956768.2 , XM_012956770.2 , XM_012956770.3 , XM_012956771.3 , XM_012956769.2 , XM_012956771.2 , NM_001006875.1
Accession Version	XM_012956771.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2007bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	E3 ubiquitin-protein ligase Midline-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012956771.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAACAC TGGAGTCTGA ACTGACCTGC CCTATCTGTC TGGAGCTGTT TGAAGATCCA 
CTGCTGCTGC CTTGTGCTCA CAGCCTGTGT TTTAACTGTG CGCATCGCAT CCTTGTCTCC 
CACTGTGCCA ACAACGAATC CGTAGAATCT ATTAACGCCT TCCAGTGCCC CACCTGCCGT 
TATGTCATCA CACTCAGCAA GCGTGGCTTA GAGGGGCTAA AGAGGAACGT GACTTTACAG 
AACATCATTG ACAGATTTCA GAAAGCCTCA GTGAGCGGGC CCAATTCACC TAGTGAAACG 
CGTCGAGAAC GTGCTTACGA TACCACTAAC AGCATGACAT CAAGCGAAAA AGTACTCTGC 
CAGTTCTGTG ATCAGGACCA GGCAAGGGAG GCTGTGAAAA CCTGTGTGAC CTGTGAAGTT 
TCATATTGTG AGGACTGTTT AAAAGCTACT CACCCAAACA AGAAACCATT CACTGGTCAC 
CGACTAATTG AACCCATCCC AGATTCTCAC ATAAGGGGGT TAATGTGCCT GGAACATGAG 
GATGAAAAAG TTAATATGTA TTGTGTGACA GATGACCAGC TAATCTGTGC CTTGTGTAAA 
TTGGTTGGGA GGCATCGAGA TCATCAAGTT GCTGCCCTTG GTGACAGATA CGACAAACTA 
AAGCAAAGCC TGGAAAGCAA CCTTACCAAC CTCATCAAAA GGAACACAGA AATGGAAATT 
CTGTTGTCCA AGCTCATTCA GACCTGCCAG CAGGTGGAGG TTAATGCATC ACGACAAGAA 
GCAAAATTAA TGGAGGAGAG TGACCAACTG ATTGAAATTA TTCAACAGAG AAGACAAATA 
ATTGGAACAA AAATAAAAGA AGGAAAGGTC GTGAGGTTAA GGAAACTGGC CCAGCAGGTT 
GCCAACTGTA AGCAGTGCAT CGAGCGCTCC ACATCCCTGA TCTCACAGGC AGAACAGTCT 
CTAAAGGAAA ACGATCACGC TCGCTTCCTT CAGTCAGCCA AGAATATCAC AGAAAGAGTC 
TCAATGGCAA CAGCATCTTC TCAAGTGCTT ATTCCAGAAA TCAACCTGAA TGACACATTT 
GACACATTTG CATTGGATTT TTCAAGAGAG AAGAAGCTGT TAGAATGTCT TGATTATCTG 
ACAGCTCCCA ATCCCCCTGT AATCAGAGAG GAACTCTGCA CTGCATCCTA CGACACCATT 
ACTGTGCATT GGACATCTGA GGATGAGTTT AGCGTGGTTT CCTATGAGCT GCAGTATACC 
ATCTTCACTG GACAAGCTAA CGTTGTTAGT TTGTGTAACT CAGCCGACAG CTGGATGATT 
GTTCCCAATA TCAAACAGAA CCATTACACA GTTCATGGTC TCCAGAGTGG CACCAAGTAC 
ATCTTCATTG TTAAGGCCAT AAACCAAGCT GGAAGTCGCA ACAGTGAACC TGGCAAGCTA 
AAAACCAACA GTCAACCTTT TAAATTAGAT CCCAAGTCTG CTCACAGGAA ACTTAAGGTA 
TCACATGACA ACCTGACCGT AGAGAGGGAC GAAACCTCTT CAAAAAAGAG CCACACACCA 
GAGCGTTTTA CAAGCCAAGG AAGTTATGGT GTTGCTGGCA ACGTGTTCCT CGACAGTGGC 
CGGCATTATT GGGAAGTGGT TATAAGCGGA AGCACCTGGT ATGCCATTGG CATTGCCTAC 
AAATCTGCAC CCAAGCACGA GTGGATAGGG AAGAATACAG CCTCTTGGGT GTTTTGCCGT 
TGTAACAACA ACTGGGCTGT AAGACACAAC AGTAAAGAGA TTCCTATAGA GCCATCTCCG 
CATCTTCGCC GCGTTGGGAT CTTGCTGGAC TACGACAATG GCTCATTGTC TTTTTATGAT 
GCTTTGAACT CCATTCACCT CTACACTTTT GACATTGCTT TTGCACAGCC CGTGTGCCCA 
ACCCTCACCG TGTGGAATAA GTGCTTAACA ATTTTAACAG GTCTCCCCAT ACCTGATCAC 
TTGGACTGCC TAGAGCAACT AGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012812225.1
CDS	130..2136
Translation	METLESELTCPICLELFEDPLLLPCAHSLCFNCAHRILVSHCANNESVESINAFQCPTCRYVITLSKRGLEGLKRNVTLQNIIDRFQKASVSGPNSPSETRRERAYDTTNSMTSSEKVLCQFCDQDQAREAVKTCVTCEVSYCEDCLKATHPNKKPFTGHRLIEPIPDSHIRGLMCLEHEDEKVNMYCVTDDQLICALCKLVGRHRDHQVAALGDRYDKLKQSLESNLTNLIKRNTEMEILLSKLIQTCQQVEVNASRQEAKLMEESDQLIEIIQQRRQIIGTKIKEGKVVRLRKLAQQVANCKQCIERSTSLISQAEQSLKENDHARFLQSAKNITERVSMATASSQVLIPEINLNDTFDTFALDFSREKKLLECLDYLTAPNPPVIREELCTASYDTITVHWTSEDEFSVVSYELQYTIFTGQANVVSLCNSADSWMIVPNIKQNHYTVHGLQSGTKYIFIVKAINQAGSRNSEPGKLKTNSQPFKLDPKSAHRKLKVSHDNLTVERDETSSKKSHTPERFTSQGSYGVAGNVFLDSGRHYWEVVISGSTWYAIGIAYKSAPKHEWIGKNTASWVFCRCNNNWAVRHNSKEIPIEPSPHLRRVGILLDYDNGSLSFYDALNSIHLYTFDIAFAQPVCPTLTVWNKCLTILTGLPIPDHLDCLEQLA

Target ORF information:

RefSeq Version	XM_012956771.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis midline 1 (mid1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012956771.3

ORF Insert Sequence:

ATGGAAACAC TGGAGTCTGA ACTGACCTGC CCTATCTGTC TGGAGCTGTT TGAAGATCCA 
CTGCTGCTGC CTTGTGCTCA CAGCCTGTGT TTTAACTGTG CGCATCGCAT CCTTGTCTCC 
CACTGTGCCA ACAACGAATC CGTAGAATCT ATTAACGCCT TCCAGTGCCC CACCTGCCGT 
TATGTCATCA CACTCAGCAA GCGTGGCTTA GAGGGGCTAA AGAGGAACGT GACTTTACAG 
AACATCATTG ACAGATTTCA GAAAGCCTCA GTGAGCGGGC CCAATTCACC TAGTGAAACG 
CGTCGAGAAC GTGCTTACGA TACCACTAAC AGCATGACAT CAAGCGAAAA AGTACTCTGC 
CAGTTCTGTG ATCAGGACCA GGCAAGGGAG GCTGTGAAAA CCTGTGTGAC CTGTGAAGTT 
TCATATTGTG AGGACTGTTT AAAAGCTACT CACCCAAACA AGAAACCATT CACTGGTCAC 
CGACTAATTG AACCCATCCC AGATTCTCAC ATAAGGGGGT TAATGTGCCT GGAACATGAG 
GATGAAAAAG TTAATATGTA TTGTGTGACA GATGACCAGC TAATCTGTGC CTTGTGTAAA 
TTGGTTGGGA GGCATCGAGA TCATCAAGTT GCTGCCCTTG GTGACAGATA CGACAAACTA 
AAGCAAAGCC TGGAAAGCAA CCTTACCAAC CTCATCAAAA GGAACACAGA AATGGAAATT 
CTGTTGTCCA AGCTCATTCA GACCTGCCAG CAGGTGGAGG TTAATGCATC ACGACAAGAA 
GCAAAATTAA TGGAGGAGAG TGACCAACTG ATTGAAATTA TTCAACAGAG AAGACAAATA 
ATTGGAACAA AAATAAAAGA AGGAAAGGTC GTGAGGTTAA GGAAACTGGC CCAGCAGGTT 
GCCAACTGTA AGCAGTGCAT CGAGCGCTCC ACATCCCTGA TCTCACAGGC AGAACAGTCT 
CTAAAGGAAA ACGATCACGC TCGCTTCCTT CAGTCAGCCA AGAATATCAC AGAAAGAGTC 
TCAATGGCAA CAGCATCTTC TCAAGTGCTT ATTCCAGAAA TCAACCTGAA TGACACATTT 
GACACATTTG CATTGGATTT TTCAAGAGAG AAGAAGCTGT TAGAATGTCT TGATTATCTG 
ACAGCTCCCA ATCCCCCTGT AATCAGAGAG GAACTCTGCA CTGCATCCTA CGACACCATT 
ACTGTGCATT GGACATCTGA GGATGAGTTT AGCGTGGTTT CCTATGAGCT GCAGTATACC 
ATCTTCACTG GACAAGCTAA CGTTGTTAGT TTGTGTAACT CAGCCGACAG CTGGATGATT 
GTTCCCAATA TCAAACAGAA CCATTACACA GTTCATGGTC TCCAGAGTGG CACCAAGTAC 
ATCTTCATTG TTAAGGCCAT AAACCAAGCT GGAAGTCGCA ACAGTGAACC TGGCAAGCTA 
AAAACCAACA GTCAACCTTT TAAATTAGAT CCCAAGTCTG CTCACAGGAA ACTTAAGGTA 
TCACATGACA ACCTGACCGT AGAGAGGGAC GAAACCTCTT CAAAAAAGAG CCACACACCA 
GAGCGTTTTA CAAGCCAAGG AAGTTATGGT GTTGCTGGCA ACGTGTTCCT CGACAGTGGC 
CGGCATTATT GGGAAGTGGT TATAAGCGGA AGCACCTGGT ATGCCATTGG CATTGCCTAC 
AAATCTGCAC CCAAGCACGA GTGGATAGGG AAGAATACAG CCTCTTGGGT GTTTTGCCGT 
TGTAACAACA ACTGGGCTGT AAGACACAAC AGTAAAGAGA TTCCTATAGA GCCATCTCCG 
CATCTTCGCC GCGTTGGGAT CTTGCTGGAC TACGACAATG GCTCATTGTC TTTTTATGAT 
GCTTTGAACT CCATTCACCT CTACACTTTT GACATTGCTT TTGCACAGCC CGTGTGCCCA 
ACCCTCACCG TGTGGAATAA GTGCTTAACA ATTTTAACAG GTCTCCCCAT ACCTGATCAC 
TTGGACTGCC TAGAGCAACT AGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mid1 ( XM_018091174.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017946663.1 Xenopus tropicalis midline 1 (mid1), transcript variant X5, mRNA.

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CloneID	OXa42507
Clone ID Related Accession (Same CDS sequence)	XM_018091174.1
Accession Version	XM_018091174.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1845bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	E3 ubiquitin-protein ligase Midline-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAACAC TGGAGTCTGA ACTGACCTGC CCTATCTGTC TGGAGCTGTT TGAAGATCCA 
CTGCTGCTGC CTTGTGCTCA CAGCCTGTGT TTTAACTGTG CGCATCGCAT CCTTGTCTCC 
CACTGTGCCA ACAACGAATC CGTAGAATCT ATTAACGCCT TCCAGTGCCC CACCTGCCGT 
TATGTCATCA CACTCAGCAA GCGTGGCTTA GAGGGGCTAA AGAGGAACGT GACTTTACAG 
AACATCATTG ACAGATTTCA GAAAGCCTCA GTGAGCGGGC CCAATTCACC TAGTGAAACG 
CGTCGAGAAC GTGCTTACGA TACCACTAAC AGCATGACAT CAAGCGAAAA AGTACTCTGC 
CAGTTCTGTG ATCAGGACCA GGCAAGGGAG GCTGTGAAAA CCTGTGTGAC CTGTGAAGTT 
TCATATTGTG AGGACTGTTT AAAAGCTACT CACCCAAACA AGAAACCATT CACTGGTCAC 
CGACTAATTG AACCCATCCC AGATTCTCAC ATAAGGGGGT TAATGTGCCT GGAACATGAG 
GATGAAAAAG TTAATATGTA TTGTGTGACA GATGACCAGC TAATCTGTGC CTTGTGTAAA 
TTGGTTGGGA GGCATCGAGA TCATCAAGTT GCTGCCCTTG GTGACAGATA CGACAAACTA 
AAGCAAAGCC TGGAAAGCAA CCTTACCAAC CTCATCAAAA GGAACACAGA AATGGAAATT 
CTGTTGTCCA AGCTCATTCA GACCTGCCAG CAGGTGGAGG TTAATGCATC ACGACAAGAA 
GCAAAATTAA TGGAGGAGAG TGACCAACTG ATTGAAATTA TTCAACAGAG AAGACAAATA 
ATTGGAACAA AAATAAAAGA AGGAAAGGTC GTGAGGTTAA GGAAACTGGC CCAGCAGGTT 
GCCAACTGTA AGCAGTGCAT CGAGCGCTCC ACATCCCTGA TCTCACAGGC AGAACAGTCT 
CTAAAGGAAA ACGATCACGC TCGCTTCCTT CAGTCAGCCA AGAATATCAC AGAAAGAGTC 
TCAATGGCAA CAGCATCTTC TCAAGTGCTT ATTCCAGAAA TCAACCTGAA TGACACATTT 
GACACATTTG CATTGGATTT TTCAAGAGAG AAGAAGCTGT TAGAATGTCT TGATTATCTG 
ACAGCTCCCA ATCCCCCTGT AATCAGAGAG GAACTCTGCA CTGCATCCTA CGACACCATT 
ACTGTGCATT GGACATCTGA GGATGAGTTT AGCGTGGTTT CCTATGAGCT GCAGTATACC 
ATCTTCACTG GACAAGCTAA CGTTGTTAGT CAACCTTTTA AATTAGATCC CAAGTCTGCT 
CACAGGAAAC TTAAGGTATC ACATGACAAC CTGACCGTAG AGAGGGACGA AACCTCTTCA 
AAAAAGAGCC ACACACCAGA GCGTTTTACA AGCCAAGGAA GTTATGGTGT TGCTGGCAAC 
GTGTTCCTCG ACAGTGGCCG GCATTATTGG GAAGTGGTTA TAAGCGGAAG CACCTGGTAT 
GCCATTGGCA TTGCCTACAA ATCTGCACCC AAGCACGAGT GGATAGGGAA GAATACAGCC 
TCTTGGGTGT TTTGCCGTTG TAACAACAAC TGGGCTGTAA GACACAACAG TAAAGAGATT 
CCTATAGAGC CATCTCCGCA TCTTCGCCGC GTTGGGATCT TGCTGGACTA CGACAATGGC 
TCATTGTCTT TTTATGATGC TTTGAACTCC ATTCACCTCT ACACTTTTGA CATTGCTTTT 
GCACAGCCCG TGTGCCCAAC CCTCACCGTG TGGAATAAGT GCTTAACAAT TTTAACAGGT 
CTCCCCATAC CTGATCACTT GGACTGCCTA GAGCAACTAG CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017946663.1
CDS	364..2208
Translation	METLESELTCPICLELFEDPLLLPCAHSLCFNCAHRILVSHCANNESVESINAFQCPTCRYVITLSKRGLEGLKRNVTLQNIIDRFQKASVSGPNSPSETRRERAYDTTNSMTSSEKVLCQFCDQDQAREAVKTCVTCEVSYCEDCLKATHPNKKPFTGHRLIEPIPDSHIRGLMCLEHEDEKVNMYCVTDDQLICALCKLVGRHRDHQVAALGDRYDKLKQSLESNLTNLIKRNTEMEILLSKLIQTCQQVEVNASRQEAKLMEESDQLIEIIQQRRQIIGTKIKEGKVVRLRKLAQQVANCKQCIERSTSLISQAEQSLKENDHARFLQSAKNITERVSMATASSQVLIPEINLNDTFDTFALDFSREKKLLECLDYLTAPNPPVIREELCTASYDTITVHWTSEDEFSVVSYELQYTIFTGQANVVSQPFKLDPKSAHRKLKVSHDNLTVERDETSSKKSHTPERFTSQGSYGVAGNVFLDSGRHYWEVVISGSTWYAIGIAYKSAPKHEWIGKNTASWVFCRCNNNWAVRHNSKEIPIEPSPHLRRVGILLDYDNGSLSFYDALNSIHLYTFDIAFAQPVCPTLTVWNKCLTILTGLPIPDHLDCLEQLA

Target ORF information:

RefSeq Version	XM_018091174.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis midline 1 (mid1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018091174.1

ORF Insert Sequence:

ATGGAAACAC TGGAGTCTGA ACTGACCTGC CCTATCTGTC TGGAGCTGTT TGAAGATCCA 
CTGCTGCTGC CTTGTGCTCA CAGCCTGTGT TTTAACTGTG CGCATCGCAT CCTTGTCTCC 
CACTGTGCCA ACAACGAATC CGTAGAATCT ATTAACGCCT TCCAGTGCCC CACCTGCCGT 
TATGTCATCA CACTCAGCAA GCGTGGCTTA GAGGGGCTAA AGAGGAACGT GACTTTACAG 
AACATCATTG ACAGATTTCA GAAAGCCTCA GTGAGCGGGC CCAATTCACC TAGTGAAACG 
CGTCGAGAAC GTGCTTACGA TACCACTAAC AGCATGACAT CAAGCGAAAA AGTACTCTGC 
CAGTTCTGTG ATCAGGACCA GGCAAGGGAG GCTGTGAAAA CCTGTGTGAC CTGTGAAGTT 
TCATATTGTG AGGACTGTTT AAAAGCTACT CACCCAAACA AGAAACCATT CACTGGTCAC 
CGACTAATTG AACCCATCCC AGATTCTCAC ATAAGGGGGT TAATGTGCCT GGAACATGAG 
GATGAAAAAG TTAATATGTA TTGTGTGACA GATGACCAGC TAATCTGTGC CTTGTGTAAA 
TTGGTTGGGA GGCATCGAGA TCATCAAGTT GCTGCCCTTG GTGACAGATA CGACAAACTA 
AAGCAAAGCC TGGAAAGCAA CCTTACCAAC CTCATCAAAA GGAACACAGA AATGGAAATT 
CTGTTGTCCA AGCTCATTCA GACCTGCCAG CAGGTGGAGG TTAATGCATC ACGACAAGAA 
GCAAAATTAA TGGAGGAGAG TGACCAACTG ATTGAAATTA TTCAACAGAG AAGACAAATA 
ATTGGAACAA AAATAAAAGA AGGAAAGGTC GTGAGGTTAA GGAAACTGGC CCAGCAGGTT 
GCCAACTGTA AGCAGTGCAT CGAGCGCTCC ACATCCCTGA TCTCACAGGC AGAACAGTCT 
CTAAAGGAAA ACGATCACGC TCGCTTCCTT CAGTCAGCCA AGAATATCAC AGAAAGAGTC 
TCAATGGCAA CAGCATCTTC TCAAGTGCTT ATTCCAGAAA TCAACCTGAA TGACACATTT 
GACACATTTG CATTGGATTT TTCAAGAGAG AAGAAGCTGT TAGAATGTCT TGATTATCTG 
ACAGCTCCCA ATCCCCCTGT AATCAGAGAG GAACTCTGCA CTGCATCCTA CGACACCATT 
ACTGTGCATT GGACATCTGA GGATGAGTTT AGCGTGGTTT CCTATGAGCT GCAGTATACC 
ATCTTCACTG GACAAGCTAA CGTTGTTAGT CAACCTTTTA AATTAGATCC CAAGTCTGCT 
CACAGGAAAC TTAAGGTATC ACATGACAAC CTGACCGTAG AGAGGGACGA AACCTCTTCA 
AAAAAGAGCC ACACACCAGA GCGTTTTACA AGCCAAGGAA GTTATGGTGT TGCTGGCAAC 
GTGTTCCTCG ACAGTGGCCG GCATTATTGG GAAGTGGTTA TAAGCGGAAG CACCTGGTAT 
GCCATTGGCA TTGCCTACAA ATCTGCACCC AAGCACGAGT GGATAGGGAA GAATACAGCC 
TCTTGGGTGT TTTGCCGTTG TAACAACAAC TGGGCTGTAA GACACAACAG TAAAGAGATT 
CCTATAGAGC CATCTCCGCA TCTTCGCCGC GTTGGGATCT TGCTGGACTA CGACAATGGC 
TCATTGTCTT TTTATGATGC TTTGAACTCC ATTCACCTCT ACACTTTTGA CATTGCTTTT 
GCACAGCCCG TGTGCCCAAC CCTCACCGTG TGGAATAAGT GCTTAACAAT TTTAACAGGT 
CTCCCCATAC CTGATCACTT GGACTGCCTA GAGCAACTAG CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	mid1
Entrez Gene ID	448651
Full Name	midline 1
Synonyms	bbbg1,fxy,gbbb1,midin,ogs1,osx,rnf59,trim18,xprf,znfxy
General protein information	Preferred Names midline 1 Names E3 ubiquitin-protein ligase Midline-1 midline 1 (Opitz/BBB syndrome)
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

Mus musculus (house mouse)	Mid1	NP_034927.2
Homo sapiens (human)	MID1	NP_150632.1
Rattus norvegicus (Norway rat)	Mid1	NP_075216.1
Danio rerio (zebrafish)	mid1	XP_002663478.1
Xenopus tropicalis (tropical clawed frog)	mid1	NP_001006876.1
Bos taurus (cattle)	MID1	NP_001179751.1
Gallus gallus (chicken)	MID1	NP_989460.1
	MID1	XP_001093390.1
Canis lupus familiaris (dog)	MID1	XP_548857.2

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

mid1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Related articles in PubMed

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