nphs2 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	nphs2
Entrez Gene ID	100498000
Full Name	NPHS2 podocin
Synonyms	nphs2l,podocin
General protein information	Preferred Names NPHS2 podocin Names podocin nephrosis 2, idiopathic, steroid-resistant (podocin)
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_018093607.1	XP_017949096.1	podocin
XM_018093607.2	XP_017949096.1	podocin

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	NPHS2	XP_005623019.1
Homo sapiens (human)	NPHS2	NP_055440.1
Pan troglodytes (chimpanzee)	NPHS2	XP_003308663.2
Bos taurus (cattle)	NPHS2	NP_001193036.1
Gallus gallus (chicken)	NPHS2	XP_422265.3
Mus musculus (house mouse)	Nphs2	NP_569723.1
Rattus norvegicus (Norway rat)	Nphs2	NP_570841.2
	NPHS2	XP_002802082.1
Danio rerio (zebrafish)	nphs2	NP_001018155.1
Xenopus tropicalis (tropical clawed frog)	nphs2	XP_002937013.2

Gene Ontology

The following nphs2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the nphs2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa09027	XM_018093607.2 Latest version!	Xenopus tropicalis NPHS2 podocin (nphs2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
View Old Accession Versions >>
OXa09027	XM_018093607.1	Xenopus tropicalis nephrosis 2, idiopathic, steroid-resistant (podocin) (nphs2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

nphs2 ( XM_018093607.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017949096.1 Xenopus tropicalis nephrosis 2, idiopathic, steroid-resistant (podocin) (nphs2), mRNA.

Price & Availability↑

CloneID	OXa09027
Clone ID Related Accession (Same CDS sequence)	XM_018093607.1 , XM_018093607.2
Accession Version	XM_018093607.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1131bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	podocin
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTAGCTA TGACTGAGAA GAGATCCCGC AGCTCCTCTC GGGAGCCTCC GCAGAAACAG 
AGGGAACACT CCAGTCAAGC CTCTAAGAGG GAGAAAAAGG CAAGCCAAGG TTCTGGAAGG 
TCCCGGAGCA GTCAAAACAG AGAATCTGCC AAAAATGATA CAAAGGTCTC TGCGGTGGTA 
GATGTTGATG ATGTTGTGGG GTCTGATGCA GAAACAGAAA TTATGGCCCT TCTTGAGACA 
AGCCAGAAGG ATGATGGTGT AAAGCCTGCA GGATTCAGAG TATTTGAAGG GCTGTTAATT 
TTCTGTTGTC TGCTGCTGGT CATCCTTACC TTGCCACTCT CTATCTGGTT CTGTGTAAAG 
GTTGTACGTG AATATGAACG TGCTGTAATA TTCCGACTGG GGCGCATGCT TTCAGGAAGG 
GCAAGAGGAC CTGGTCTCTT CTTTTACCTA CCCTGCTTGG ACAAGTGCCA TAAAGTAGAC 
TTTCGTTTGA AGACTTTTGA AGTTCCTTTT CACCAGATTG TGACAAAGGA CCTGGTTACT 
TTGGAGATTG ATGTTATCTG TTACTATCGC TTGGAAAATG CCTGTCTTTT TCTAACCAGT 
GTCAGCAGCA TCTCCAGTGC TTTCCAGTTG TTGGTCCAGA CTACCACCAA ACGGCTCTTG 
GCACACCGAG CTTTCTTAGA TATCTTACTG GAAAGAAAGA GTATTGGGGA GGAAGTGAAG 
GTGGCTCTGG ATGCAGCTAC CTGTCACTGG GGAATCAAAG TGGAAAGAAC TGAAATTAAG 
GATGTAAAAC TTCCAGAAGA GGTAAAGCAA TCTATGGCAG TAGAAGCAGA AGCACAAAGG 
CATGCTAAAG TCAAGGTAAT TGCTGCAGAA GGAGAGAAAA CAGTATCTGA ATACATTAAG 
CTGGCTGCTG AAAAACTATC TGGATCCCCA ACAGCAATTC AGTTAAGATA TCTGCACACA 
CTGCAATGTA TGACTTCGGA AAAACCAGCC ACATTTGTCT TGCCTTTACC CTTTGACTTA 
ATGAACCTGA ATGCAGTGGC CAGATTGCCT GAGATTAATC CAACCCCAAA TACTCCTAAA 
TCAGATAATG ATACTGAACA GGGGACAAAG AAGGACTCTC CAATGCTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017949096.1
CDS	52..1182
Translation	MLAMTEKRSRSSSREPPQKQREHSSQASKREKKASQGSGRSRSSQNRESAKNDTKVSAVVDVDDVVGSDAETEIMALLETSQKDDGVKPAGFRVFEGLLIFCCLLLVILTLPLSIWFCVKVVREYERAVIFRLGRMLSGRARGPGLFFYLPCLDKCHKVDFRLKTFEVPFHQIVTKDLVTLEIDVICYYRLENACLFLTSVSSISSAFQLLVQTTTKRLLAHRAFLDILLERKSIGEEVKVALDAATCHWGIKVERTEIKDVKLPEEVKQSMAVEAEAQRHAKVKVIAAEGEKTVSEYIKLAAEKLSGSPTAIQLRYLHTLQCMTSEKPATFVLPLPFDLMNLNAVARLPEINPTPNTPKSDNDTEQGTKKDSPML

Target ORF information:

RefSeq Version	XM_018093607.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis nephrosis 2, idiopathic, steroid-resistant (podocin) (nphs2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018093607.1

ORF Insert Sequence:

ATGTTAGCTA TGACTGAGAA GAGATCCCGC AGCTCCTCTC GGGAGCCTCC GCAGAAACAG 
AGGGAACACT CCAGTCAAGC CTCTAAGAGG GAGAAAAAGG CAAGCCAAGG TTCTGGAAGG 
TCCCGGAGCA GTCAAAACAG AGAATCTGCC AAAAATGATA CAAAGGTCTC TGCGGTGGTA 
GATGTTGATG ATGTTGTGGG GTCTGATGCA GAAACAGAAA TTATGGCCCT TCTTGAGACA 
AGCCAGAAGG ATGATGGTGT AAAGCCTGCA GGATTCAGAG TATTTGAAGG GCTGTTAATT 
TTCTGTTGTC TGCTGCTGGT CATCCTTACC TTGCCACTCT CTATCTGGTT CTGTGTAAAG 
GTTGTACGTG AATATGAACG TGCTGTAATA TTCCGACTGG GGCGCATGCT TTCAGGAAGG 
GCAAGAGGAC CTGGTCTCTT CTTTTACCTA CCCTGCTTGG ACAAGTGCCA TAAAGTAGAC 
TTTCGTTTGA AGACTTTTGA AGTTCCTTTT CACCAGATTG TGACAAAGGA CCTGGTTACT 
TTGGAGATTG ATGTTATCTG TTACTATCGC TTGGAAAATG CCTGTCTTTT TCTAACCAGT 
GTCAGCAGCA TCTCCAGTGC TTTCCAGTTG TTGGTCCAGA CTACCACCAA ACGGCTCTTG 
GCACACCGAG CTTTCTTAGA TATCTTACTG GAAAGAAAGA GTATTGGGGA GGAAGTGAAG 
GTGGCTCTGG ATGCAGCTAC CTGTCACTGG GGAATCAAAG TGGAAAGAAC TGAAATTAAG 
GATGTAAAAC TTCCAGAAGA GGTAAAGCAA TCTATGGCAG TAGAAGCAGA AGCACAAAGG 
CATGCTAAAG TCAAGGTAAT TGCTGCAGAA GGAGAGAAAA CAGTATCTGA ATACATTAAG 
CTGGCTGCTG AAAAACTATC TGGATCCCCA ACAGCAATTC AGTTAAGATA TCTGCACACA 
CTGCAATGTA TGACTTCGGA AAAACCAGCC ACATTTGTCT TGCCTTTACC CTTTGACTTA 
ATGAACCTGA ATGCAGTGGC CAGATTGCCT GAGATTAATC CAACCCCAAA TACTCCTAAA 
TCAGATAATG ATACTGAACA GGGGACAAAG AAGGACTCTC CAATGCTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nphs2 ( XM_018093607.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017949096.1 Xenopus tropicalis NPHS2 podocin (nphs2), mRNA.

Price & Availability↑

CloneID	OXa09027
Clone ID Related Accession (Same CDS sequence)	XM_018093607.1 , XM_018093607.2
Accession Version	XM_018093607.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1131bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	podocin
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_018093607.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTAGCTA TGACTGAGAA GAGATCCCGC AGCTCCTCTC GGGAGCCTCC GCAGAAACAG 
AGGGAACACT CCAGTCAAGC CTCTAAGAGG GAGAAAAAGG CAAGCCAAGG TTCTGGAAGG 
TCCCGGAGCA GTCAAAACAG AGAATCTGCC AAAAATGATA CAAAGGTCTC TGCGGTGGTA 
GATGTTGATG ATGTTGTGGG GTCTGATGCA GAAACAGAAA TTATGGCCCT TCTTGAGACA 
AGCCAGAAGG ATGATGGTGT AAAGCCTGCA GGATTCAGAG TATTTGAAGG GCTGTTAATT 
TTCTGTTGTC TGCTGCTGGT CATCCTTACC TTGCCACTCT CTATCTGGTT CTGTGTAAAG 
GTTGTACGTG AATATGAACG TGCTGTAATA TTCCGACTGG GGCGCATGCT TTCAGGAAGG 
GCAAGAGGAC CTGGTCTCTT CTTTTACCTA CCCTGCTTGG ACAAGTGCCA TAAAGTAGAC 
TTTCGTTTGA AGACTTTTGA AGTTCCTTTT CACCAGATTG TGACAAAGGA CCTGGTTACT 
TTGGAGATTG ATGTTATCTG TTACTATCGC TTGGAAAATG CCTGTCTTTT TCTAACCAGT 
GTCAGCAGCA TCTCCAGTGC TTTCCAGTTG TTGGTCCAGA CTACCACCAA ACGGCTCTTG 
GCACACCGAG CTTTCTTAGA TATCTTACTG GAAAGAAAGA GTATTGGGGA GGAAGTGAAG 
GTGGCTCTGG ATGCAGCTAC CTGTCACTGG GGAATCAAAG TGGAAAGAAC TGAAATTAAG 
GATGTAAAAC TTCCAGAAGA GGTAAAGCAA TCTATGGCAG TAGAAGCAGA AGCACAAAGG 
CATGCTAAAG TCAAGGTAAT TGCTGCAGAA GGAGAGAAAA CAGTATCTGA ATACATTAAG 
CTGGCTGCTG AAAAACTATC TGGATCCCCA ACAGCAATTC AGTTAAGATA TCTGCACACA 
CTGCAATGTA TGACTTCGGA AAAACCAGCC ACATTTGTCT TGCCTTTACC CTTTGACTTA 
ATGAACCTGA ATGCAGTGGC CAGATTGCCT GAGATTAATC CAACCCCAAA TACTCCTAAA 
TCAGATAATG ATACTGAACA GGGGACAAAG AAGGACTCTC CAATGCTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017949096.1
CDS	141..1271
Translation	MLAMTEKRSRSSSREPPQKQREHSSQASKREKKASQGSGRSRSSQNRESAKNDTKVSAVVDVDDVVGSDAETEIMALLETSQKDDGVKPAGFRVFEGLLIFCCLLLVILTLPLSIWFCVKVVREYERAVIFRLGRMLSGRARGPGLFFYLPCLDKCHKVDFRLKTFEVPFHQIVTKDLVTLEIDVICYYRLENACLFLTSVSSISSAFQLLVQTTTKRLLAHRAFLDILLERKSIGEEVKVALDAATCHWGIKVERTEIKDVKLPEEVKQSMAVEAEAQRHAKVKVIAAEGEKTVSEYIKLAAEKLSGSPTAIQLRYLHTLQCMTSEKPATFVLPLPFDLMNLNAVARLPEINPTPNTPKSDNDTEQGTKKDSPML

Target ORF information:

RefSeq Version	XM_018093607.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis NPHS2 podocin (nphs2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018093607.2

ORF Insert Sequence:

ATGTTAGCTA TGACTGAGAA GAGATCCCGC AGCTCCTCTC GGGAGCCTCC GCAGAAACAG 
AGGGAACACT CCAGTCAAGC CTCTAAGAGG GAGAAAAAGG CAAGCCAAGG TTCTGGAAGG 
TCCCGGAGCA GTCAAAACAG AGAATCTGCC AAAAATGATA CAAAGGTCTC TGCGGTGGTA 
GATGTTGATG ATGTTGTGGG GTCTGATGCA GAAACAGAAA TTATGGCCCT TCTTGAGACA 
AGCCAGAAGG ATGATGGTGT AAAGCCTGCA GGATTCAGAG TATTTGAAGG GCTGTTAATT 
TTCTGTTGTC TGCTGCTGGT CATCCTTACC TTGCCACTCT CTATCTGGTT CTGTGTAAAG 
GTTGTACGTG AATATGAACG TGCTGTAATA TTCCGACTGG GGCGCATGCT TTCAGGAAGG 
GCAAGAGGAC CTGGTCTCTT CTTTTACCTA CCCTGCTTGG ACAAGTGCCA TAAAGTAGAC 
TTTCGTTTGA AGACTTTTGA AGTTCCTTTT CACCAGATTG TGACAAAGGA CCTGGTTACT 
TTGGAGATTG ATGTTATCTG TTACTATCGC TTGGAAAATG CCTGTCTTTT TCTAACCAGT 
GTCAGCAGCA TCTCCAGTGC TTTCCAGTTG TTGGTCCAGA CTACCACCAA ACGGCTCTTG 
GCACACCGAG CTTTCTTAGA TATCTTACTG GAAAGAAAGA GTATTGGGGA GGAAGTGAAG 
GTGGCTCTGG ATGCAGCTAC CTGTCACTGG GGAATCAAAG TGGAAAGAAC TGAAATTAAG 
GATGTAAAAC TTCCAGAAGA GGTAAAGCAA TCTATGGCAG TAGAAGCAGA AGCACAAAGG 
CATGCTAAAG TCAAGGTAAT TGCTGCAGAA GGAGAGAAAA CAGTATCTGA ATACATTAAG 
CTGGCTGCTG AAAAACTATC TGGATCCCCA ACAGCAATTC AGTTAAGATA TCTGCACACA 
CTGCAATGTA TGACTTCGGA AAAACCAGCC ACATTTGTCT TGCCTTTACC CTTTGACTTA 
ATGAACCTGA ATGCAGTGGC CAGATTGCCT GAGATTAATC CAACCCCAAA TACTCCTAAA 
TCAGATAATG ATACTGAACA GGGGACAAAG AAGGACTCTC CAATGCTTTA A