topaz1 gene cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

mRNA	Protein	Name
XM_012964409.2	XP_012819863.1	testis- and ovary-specific PAZ domain-containing protein 1 isoform X1
NM_001374744.1	NP_001361673.1	testis- and ovary-specific PAZ domain-containing protein 1
XM_031903275.1	XP_031759135.1	protein TOPAZ1 isoform X1
XM_018094576.1	XP_017950065.1	testis- and ovary-specific PAZ domain-containing protein 1 isoform X4
XM_012964412.2	XP_012819866.1	testis- and ovary-specific PAZ domain-containing protein 1 isoform X2
XM_012964413.2	XP_012819867.1	testis- and ovary-specific PAZ domain-containing protein 1 isoform X2
XM_018094575.2	XP_017950064.2	protein TOPAZ1 isoform X1
XM_031903276.1	XP_031759136.1	protein TOPAZ1 isoform X1
XM_018094575.1	XP_017950064.1	testis- and ovary-specific PAZ domain-containing protein 1 isoform X3
XM_012964411.2	XP_012819865.1	testis- and ovary-specific PAZ domain-containing protein 1 isoform X2
NM_001199939.1	NP_001186868.1	protein TOPAZ1

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa24852	NM_001199939.1 Latest version!	Xenopus tropicalis testis and ovary specific PAZ domain containing 1 (topaz1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OXa44875	XM_018094575.1 Latest version!	Xenopus tropicalis testis and ovary specific PAZ domain containing 1 (topaz1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OXa44876	XM_018094576.1 Latest version!	Xenopus tropicalis testis and ovary specific PAZ domain containing 1 (topaz1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OXa59574	XM_031903275.1 Latest version!	Xenopus tropicalis testis and ovary specific PAZ domain containing 1 (topaz1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OXa59574	XM_018094575.2 Latest version!	Xenopus tropicalis testis and ovary specific PAZ domain containing 1 (topaz1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OXa59574	XM_031903276.1 Latest version!	Xenopus tropicalis testis and ovary specific PAZ domain containing 1 (topaz1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OXa33111	NM_001374744.1 Latest version!	Xenopus tropicalis testis and ovary specific PAZ domain containing 1 (topaz1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa33111	XM_012964409.2 Latest version!	Xenopus tropicalis testis and ovary specific PAZ domain containing 1 (topaz1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OXa33112	XM_012964413.2 Latest version!	Xenopus tropicalis testis and ovary specific PAZ domain containing 1 (topaz1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OXa33112	XM_012964412.2 Latest version!	Xenopus tropicalis testis and ovary specific PAZ domain containing 1 (topaz1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OXa33112	XM_012964411.2 Latest version!	Xenopus tropicalis testis and ovary specific PAZ domain containing 1 (topaz1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00

topaz1 ( NM_001199939.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> NP_001186868.1 Xenopus tropicalis testis and ovary specific PAZ domain containing 1 (topaz1), mRNA.

Price & Availability↑

CloneID	OXa24852
Clone ID Related Accession (Same CDS sequence)	NM_001199939.1
Accession Version	NM_001199939.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2490bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-21
Organism	Xenopus tropicalis(tropical clawed frog)
Product	protein TOPAZ1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL895749.2, EL833478.1, DN087003.1, BX756929.1, BX770256.1, DT402254.1, DT402253.1 and EL833479.1. On Jan 14, 2011 this sequence version replaced XM_002937848.1. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00028290, SAMD00028291 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTTCTACA CACAACTGTT ATTTCACTGT TACAGATCCA GCACACACGA TGTTGTTCCA 
GATGATCCAG AGCTTTTTGG GACTCCTGAA GATCCTTACA TAAGTCCAAT TTGTAATGCT 
GACAACAAAG AAAATAATAA ACGGAATAAT CTGAGTGCAT TAATGTGTAA CCTTCAGGAA 
CAGGAGAGCG CCCATATCAA TGAAAGCACA AATGATGCCA GCCCTGAGGA AAATGTAGAA 
GAGAGAAGTA CATATTTTGC AAAAGTAATC ACTGATCTAG AAGTTAATGA TGATCCTCTC 
ATCATACATA ACACTTTGGA AAAGAACAGT GAAGATTTTT ATGATGGTGG ACAGTTTGAT 
TTTGAGGCGT TCAGAGATCT TGACGCAGAT AAAGAGATTA AATATGCTGA AAAGAATTCA 
TCTGAAAGCG GAAGCAGTTT TGAAGAGTCC TTGTCCAAGG ATACTTCTCC GGGGTCCTCT 
CATTCCAATA GCCGTCGTTG GAGAAGGGAT CCCTCACATA CCTATAAGCC TATGCATTGG 
ATGAATGATT TCAGATACTC TGGAAAATGC ATGGGATTGA CGGATGTGAT TACTTTGGAT 
CAGAAAGAAC AGAGGAATAT TGATAATCTC TTTTACGATT TTACACCTAT ACAGAAGACC 
TTTTTGCCCA TTAGATACTG CAAATATTTT TTTAATACTT TCCGTGGATG CATTAAACCA 
GATTGTATGT ACCAGCACGT ACCATTCCAA AGGGATGAAA AGGTGTGCAT GGAGGTGATT 
CACAAACTTG TGAATGAAAA TCACACAACA TTATTAAAAC GTGCTGTTTG GATATTTACT 
GCCTACTACA GAATGTATAT GCCTGGTGTT CATTATGACT CAAATCTGCT GACCAAAATG 
CTCCGTGCCT TGTATGTTCG TCAGATGTGG GGAGATGTAT TTCAGTTGTT GGAAACTGGT 
GCCAATGTCA AAATATTGCC CTCCTCTGAA ATGCTTATCA GGTTATTTGA AAATATTGGC 
TCTACGGGAT TAACAGCAGC TGTACCTAGT TTAGTGGATG TATTCTGCAA GCTTGTGGAG 
GCTGGAATGA TGATGAAGCC AGAGCAAATC AACATGTTAA TAACAACACT GAATAATCTC 
CAGGCAACAA AAAATTATAT CAGTGTCATT TTAGACATAA AAACAAGAAT TGAAATGCAG 
CTTTCAGAAA AGAACTGGCT GTGTAACTTG GATATAGCAG TTGCAGAAGT TGGGCATTGT 
AAAGAAAAAA ATGATTGGAA GAAACTAGGA ACATTATATC TTAATCTGCG TACAGGGTGT 
GAGAATGTGA CTGATCTTAA GAAGTTTTCT AATTGCATTG TGGGGGCGCT TCAGAAAGTG 
CCAAGGAATG ATAAATCTGA AATTCCATAT TGTGACTTTG CAGACACAGT GTACAAAGAC 
GCCCAACTAA GTGAAATTGA CAAAAACATT TTGGGAAGGA TTGGAATATC CATCATGTAT 
CACTATTACA GAAATAAACA ATCGCAAAAG GGAAAGAGGG TGCTCAGAAA ACTTCAGGAA 
ATGCAGATTA ATTTCACAGT CCTAAAAGGA CTCACAGGGG AAGAGAGTAA AGCTACGCGC 
TGTCAAGTTG TGAACACAGC TGTAGAGATC TTCTTGAACT GTGAATACTT AAATGGTGCA 
CTGGGGGTTC TACGAGAGTC AGAGTGGATC ATTAACACAC AAGTGTGGCC ATGTGAAAGA 
ATGGATGTGC TGAAACGACA TAACCTGTTG TGTACAATTG CACAGAAAAC ACTGAATAAA 
AACATGTTTG ATGTGTGTCT TGAGGTCCTT CAGAATCTGC CAGGACTGCA GTGCTCATTA 
ACGGATGTGG ACGTCTCTCA GTACAGCTTG CTTTTCAACA AACTTTTGTC ATCCTGCATA 
GAAAATAATA CACTTGGTGT ATCCTCAACT GTGATAGATT TTATGATTGC AAAAAAAATA 
CCACTTGACT TTTTCTTACT GAGAGCACTA ATCACATCCT TGGGACGAAG CTGTCTATGG 
CTCAGAGCAA GAGAACTTTA TAAATGCGCT GTTTTGTTGG GTTGCTACCC GCCCATGGAA 
GGAAATCTGT ATCGTAAAGT GCTCTTCATC CCCTCATTCC TTACTGAGAT AGAAATGCTG 
TTATCCATTG AAATTTTCAT GGTGTCAAAT GCCAGTAGTA TTCAGTCACC AGGAGGCAGC 
CATCAGACTC TGCAAATCAT TCTCAAAAGA TGTGAAGAAG AGAGAGTTCC GAATAAAGAT 
TATCAAAGAG GTAAAGCTAG CTATCAAGAT GCAGTGGACC GGCTGATTCA GGCCTCTAGG 
CTATCAACAC CAAGATTATT CATAAAGCAC CTGACTGTCA ATAATGCCAA TGAACAGGTT 
TATATCCTTG ATTACGGCAG CTCTCTCAAA TGGTTGCATG AAAACATGAA ATGGGCTGGG 
AAAGTATGGC TTTTTCAGGG GAAAATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001186868.1
CDS	189..2678
Translation	MFYTQLLFHCYRSSTHDVVPDDPELFGTPEDPYISPICNADNKENNKRNNLSALMCNLQEQESAHINESTNDASPEENVEERSTYFAKVITDLEVNDDPLIIHNTLEKNSEDFYDGGQFDFEAFRDLDADKEIKYAEKNSSESGSSFEESLSKDTSPGSSHSNSRRWRRDPSHTYKPMHWMNDFRYSGKCMGLTDVITLDQKEQRNIDNLFYDFTPIQKTFLPIRYCKYFFNTFRGCIKPDCMYQHVPFQRDEKVCMEVIHKLVNENHTTLLKRAVWIFTAYYRMYMPGVHYDSNLLTKMLRALYVRQMWGDVFQLLETGANVKILPSSEMLIRLFENIGSTGLTAAVPSLVDVFCKLVEAGMMMKPEQINMLITTLNNLQATKNYISVILDIKTRIEMQLSEKNWLCNLDIAVAEVGHCKEKNDWKKLGTLYLNLRTGCENVTDLKKFSNCIVGALQKVPRNDKSEIPYCDFADTVYKDAQLSEIDKNILGRIGISIMYHYYRNKQSQKGKRVLRKLQEMQINFTVLKGLTGEESKATRCQVVNTAVEIFLNCEYLNGALGVLRESEWIINTQVWPCERMDVLKRHNLLCTIAQKTLNKNMFDVCLEVLQNLPGLQCSLTDVDVSQYSLLFNKLLSSCIENNTLGVSSTVIDFMIAKKIPLDFFLLRALITSLGRSCLWLRARELYKCAVLLGCYPPMEGNLYRKVLFIPSFLTEIEMLLSIEIFMVSNASSIQSPGGSHQTLQIILKRCEEERVPNKDYQRGKASYQDAVDRLIQASRLSTPRLFIKHLTVNNANEQVYILDYGSSLKWLHENMKWAGKVWLFQGKI

Target ORF information:

RefSeq Version	NM_001199939.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis testis and ovary specific PAZ domain containing 1 (topaz1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001199939.1

ORF Insert Sequence:

ATGTTCTACA CACAACTGTT ATTTCACTGT TACAGATCCA GCACACACGA TGTTGTTCCA 
GATGATCCAG AGCTTTTTGG GACTCCTGAA GATCCTTACA TAAGTCCAAT TTGTAATGCT 
GACAACAAAG AAAATAATAA ACGGAATAAT CTGAGTGCAT TAATGTGTAA CCTTCAGGAA 
CAGGAGAGCG CCCATATCAA TGAAAGCACA AATGATGCCA GCCCTGAGGA AAATGTAGAA 
GAGAGAAGTA CATATTTTGC AAAAGTAATC ACTGATCTAG AAGTTAATGA TGATCCTCTC 
ATCATACATA ACACTTTGGA AAAGAACAGT GAAGATTTTT ATGATGGTGG ACAGTTTGAT 
TTTGAGGCGT TCAGAGATCT TGACGCAGAT AAAGAGATTA AATATGCTGA AAAGAATTCA 
TCTGAAAGCG GAAGCAGTTT TGAAGAGTCC TTGTCCAAGG ATACTTCTCC GGGGTCCTCT 
CATTCCAATA GCCGTCGTTG GAGAAGGGAT CCCTCACATA CCTATAAGCC TATGCATTGG 
ATGAATGATT TCAGATACTC TGGAAAATGC ATGGGATTGA CGGATGTGAT TACTTTGGAT 
CAGAAAGAAC AGAGGAATAT TGATAATCTC TTTTACGATT TTACACCTAT ACAGAAGACC 
TTTTTGCCCA TTAGATACTG CAAATATTTT TTTAATACTT TCCGTGGATG CATTAAACCA 
GATTGTATGT ACCAGCACGT ACCATTCCAA AGGGATGAAA AGGTGTGCAT GGAGGTGATT 
CACAAACTTG TGAATGAAAA TCACACAACA TTATTAAAAC GTGCTGTTTG GATATTTACT 
GCCTACTACA GAATGTATAT GCCTGGTGTT CATTATGACT CAAATCTGCT GACCAAAATG 
CTCCGTGCCT TGTATGTTCG TCAGATGTGG GGAGATGTAT TTCAGTTGTT GGAAACTGGT 
GCCAATGTCA AAATATTGCC CTCCTCTGAA ATGCTTATCA GGTTATTTGA AAATATTGGC 
TCTACGGGAT TAACAGCAGC TGTACCTAGT TTAGTGGATG TATTCTGCAA GCTTGTGGAG 
GCTGGAATGA TGATGAAGCC AGAGCAAATC AACATGTTAA TAACAACACT GAATAATCTC 
CAGGCAACAA AAAATTATAT CAGTGTCATT TTAGACATAA AAACAAGAAT TGAAATGCAG 
CTTTCAGAAA AGAACTGGCT GTGTAACTTG GATATAGCAG TTGCAGAAGT TGGGCATTGT 
AAAGAAAAAA ATGATTGGAA GAAACTAGGA ACATTATATC TTAATCTGCG TACAGGGTGT 
GAGAATGTGA CTGATCTTAA GAAGTTTTCT AATTGCATTG TGGGGGCGCT TCAGAAAGTG 
CCAAGGAATG ATAAATCTGA AATTCCATAT TGTGACTTTG CAGACACAGT GTACAAAGAC 
GCCCAACTAA GTGAAATTGA CAAAAACATT TTGGGAAGGA TTGGAATATC CATCATGTAT 
CACTATTACA GAAATAAACA ATCGCAAAAG GGAAAGAGGG TGCTCAGAAA ACTTCAGGAA 
ATGCAGATTA ATTTCACAGT CCTAAAAGGA CTCACAGGGG AAGAGAGTAA AGCTACGCGC 
TGTCAAGTTG TGAACACAGC TGTAGAGATC TTCTTGAACT GTGAATACTT AAATGGTGCA 
CTGGGGGTTC TACGAGAGTC AGAGTGGATC ATTAACACAC AAGTGTGGCC ATGTGAAAGA 
ATGGATGTGC TGAAACGACA TAACCTGTTG TGTACAATTG CACAGAAAAC ACTGAATAAA 
AACATGTTTG ATGTGTGTCT TGAGGTCCTT CAGAATCTGC CAGGACTGCA GTGCTCATTA 
ACGGATGTGG ACGTCTCTCA GTACAGCTTG CTTTTCAACA AACTTTTGTC ATCCTGCATA 
GAAAATAATA CACTTGGTGT ATCCTCAACT GTGATAGATT TTATGATTGC AAAAAAAATA 
CCACTTGACT TTTTCTTACT GAGAGCACTA ATCACATCCT TGGGACGAAG CTGTCTATGG 
CTCAGAGCAA GAGAACTTTA TAAATGCGCT GTTTTGTTGG GTTGCTACCC GCCCATGGAA 
GGAAATCTGT ATCGTAAAGT GCTCTTCATC CCCTCATTCC TTACTGAGAT AGAAATGCTG 
TTATCCATTG AAATTTTCAT GGTGTCAAAT GCCAGTAGTA TTCAGTCACC AGGAGGCAGC 
CATCAGACTC TGCAAATCAT TCTCAAAAGA TGTGAAGAAG AGAGAGTTCC GAATAAAGAT 
TATCAAAGAG GTAAAGCTAG CTATCAAGAT GCAGTGGACC GGCTGATTCA GGCCTCTAGG 
CTATCAACAC CAAGATTATT CATAAAGCAC CTGACTGTCA ATAATGCCAA TGAACAGGTT 
TATATCCTTG ATTACGGCAG CTCTCTCAAA TGGTTGCATG AAAACATGAA ATGGGCTGGG 
AAAGTATGGC TTTTTCAGGG GAAAATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

topaz1 ( XM_018094575.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017950064.1 Xenopus tropicalis testis and ovary specific PAZ domain containing 1 (topaz1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OXa44875
Clone ID Related Accession (Same CDS sequence)	XM_018094575.1
Accession Version	XM_018094575.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2490bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	testis- and ovary-specific PAZ domain-containing protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCTACA CACAACTGTT ATTTCACTGT TACAGATCCA GCACACACGA TGTTGTTCCA 
GATGATCCAG AGCTTTTTGG GACTCCTGAA GATCCTTACA TAAGTCCAAT TTGTAATGCT 
GACAACAAAG AAAATAATAA ACGGAATAAT CTGAGTGCAT TAATGTGTAA CCTTCAGGAA 
CAGGAGAGCG CCCATATCAA TGAAAGCACA AATGATGCCA GCCCTGAGGA AAATGTAGAA 
GAGAGAAGTA CATATTTTGC AAAAGTAATC ACTGATCTAG AAGTTAATGA TGATCCTCTC 
ATCATACATA ACACTTTGGA AAAGAACAGT GAAGATTTTT ATGATGGTGG ACAGTTTGAT 
TTTGAGGCGT TCAGAGATCT TGACGCAGAT AAAGAGATTA AATATGCTGA AAAGAATTCA 
TCTGAAAGCG GAAGCAGTTT TGAAGAGTCC TTGTCCAAGG ATACTTCTCC GGGGTCCTCT 
CATTCCAATA GCCGTCGTTG GAGAAGAGAT CCCTCACATA CCTATAAGCC TATGCATTGG 
ATGAATGATT TCAGATACTC TGGAAAATGC ATGGGATTGA CGGATGTGAT TACTTTGGAT 
CAGAAAGAAC AGAGGAATAT TGATAATCTC TTTTACGATT TTACACCTAT ACAGAAGACC 
TTTTTGCCCA TTAGATACTG CAAATATTTT TTTAATACTT TCCGTGGATG CATTAAACCA 
GATTGTATGT ACCAGCACGT ACCATTCCAA AGGGATGAAA AGGTGTGCAT GGAGGTGATT 
CACAAACTTG TGAATGAAAA TCACACAACA TTATTAAAAC GTGCTGTTTG GATATTTACT 
GCCTACTACA GAATGTATAT GCCTGGTGTT CATTATGACT CAAATCTGCT GACCAAAATG 
CTCCGTGCCT TGTATGTTCG TCAGATGTGG GGAGATGTAT TTCAGTTGTT GGAAACTGGT 
GCCAATGTCA AAATATTGCC CTCCTCTGAA ATGCTTATCA GGTTATTTGA AAATATTGGC 
TCTACGGGAT TAACAGCAGC TGTACCTAGT TTAGTGGATG TATTCTGCAA GCTTGTGGAG 
GCTGGAATGA TGATGAAGCC AGAGCAAATC AACATGTTAA TAACAACACT GAATAATCTC 
CAGGCAACAA AAAATTATAT CAGTGTCATT TTAGACATAA AAACAAGAAT TGAAATGCAG 
CTTTCAGAAA AGAACTGGCT GTGTAACTTG GATATAGCAG TTGCAGAAGT TGGGCATTGT 
AAAGAAAAAA ATGATTGGAA GAAACTAGGA ACATTATATC TTAATCTGCG TACAGGGTGT 
GAGAATGTGA CTGATCTTAA GAAGTTTTCT AATTGCATTG TGGGGGCGCT TCAGAAAGTG 
CCAAGGAATG ATAAATCTGA AATTCCATAT TGTGACTTTG CAGACACAGT GTACAAAGAC 
GCCCAACTAA GTGAAATTGA CAAAAACATT TTGGGAAGGA TTGGAATATC CATCATGTAT 
CACTATTACA GAAATAAACA ATCGCAAAAG GGAAAGAGGG TGCTCAGAAA ACTTCAGGAA 
ATGCAGATTA ATTTCACAGT CCTAAAAGGA CTCACAGGGG AAGAGAGTAA AGCTACGCGC 
TGTCAAGTTG TGAACACAGC TGTAGAGATC TTCTTGAACT GTGAATACTT AAATGGTGCA 
CTGGGGGTTC TACGAGAGTC AGAGTGGATC ATTAACACAC AAGTGTGGCC ATGTGAAAGA 
ATGGATGTGC TGAAACGACA TAACCTGTTG TGTACAATTG CACAGAAAAC ACTGAATAAA 
AACATGTTTG ATGTGTGTCT TGAGGTCCTT CAGAATCTGC CAGGACTGCA GTGCTCATTA 
ACGGATGTGG ACGTCTCTCA GTACAGCTTG CTTTTCAACA AACTTTTGTC ATCCTGCATA 
GAAAATAATA CACTTGGTGT ATCCTCAACT GTGATAGATT TTATGATTGC AAAAAAAATA 
CCACTTGACT TTTTCTTACT GAGAGCACTA ATCACATCCT TGGGACGAAG CTGTCTATGG 
CTCAGAGCAA GAGAACTTTA TAAATGCGCT GTTTTGTTGG GTTGCTACCC GCCCATGGAA 
GGAAATCTGT ATCGTAAAGT GCTCTTCATC CCCTCATTCC TTACTGAGAT AGAAATGCTG 
TTATCCATTG AAATTTTCAT GGTGTCAAAT GCCAGTAGTA TTCAGTCACC AGGAGGCAGC 
CATCAGACTC TGCAAATCAT TCTCAAAAGA TGTGAAGAAG AGAGAGTTCC GAATAAAGAT 
TATCAAAGAG GTAAAGCTAG CTATCAAGAT GCAGTGGACC GGCTGATTCA GGCCTCTAGG 
CTATCAACAC CAAGATTATT CATAAAGCAC CTGACTGTCA ATAATGCCAA TGAACAGGTT 
TATATCCTTG ATTACGGCAG CTCTCTCAAA TGGTTGCATG AAAACATGAA ATGGGCTGGG 
AAAGTATGGC TTTTTCAGGG GAAAATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017950064.1
CDS	162..2651
Translation	MFYTQLLFHCYRSSTHDVVPDDPELFGTPEDPYISPICNADNKENNKRNNLSALMCNLQEQESAHINESTNDASPEENVEERSTYFAKVITDLEVNDDPLIIHNTLEKNSEDFYDGGQFDFEAFRDLDADKEIKYAEKNSSESGSSFEESLSKDTSPGSSHSNSRRWRRDPSHTYKPMHWMNDFRYSGKCMGLTDVITLDQKEQRNIDNLFYDFTPIQKTFLPIRYCKYFFNTFRGCIKPDCMYQHVPFQRDEKVCMEVIHKLVNENHTTLLKRAVWIFTAYYRMYMPGVHYDSNLLTKMLRALYVRQMWGDVFQLLETGANVKILPSSEMLIRLFENIGSTGLTAAVPSLVDVFCKLVEAGMMMKPEQINMLITTLNNLQATKNYISVILDIKTRIEMQLSEKNWLCNLDIAVAEVGHCKEKNDWKKLGTLYLNLRTGCENVTDLKKFSNCIVGALQKVPRNDKSEIPYCDFADTVYKDAQLSEIDKNILGRIGISIMYHYYRNKQSQKGKRVLRKLQEMQINFTVLKGLTGEESKATRCQVVNTAVEIFLNCEYLNGALGVLRESEWIINTQVWPCERMDVLKRHNLLCTIAQKTLNKNMFDVCLEVLQNLPGLQCSLTDVDVSQYSLLFNKLLSSCIENNTLGVSSTVIDFMIAKKIPLDFFLLRALITSLGRSCLWLRARELYKCAVLLGCYPPMEGNLYRKVLFIPSFLTEIEMLLSIEIFMVSNASSIQSPGGSHQTLQIILKRCEEERVPNKDYQRGKASYQDAVDRLIQASRLSTPRLFIKHLTVNNANEQVYILDYGSSLKWLHENMKWAGKVWLFQGKI

Target ORF information:

RefSeq Version	XM_018094575.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis testis and ovary specific PAZ domain containing 1 (topaz1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018094575.1

ORF Insert Sequence:

ATGTTCTACA CACAACTGTT ATTTCACTGT TACAGATCCA GCACACACGA TGTTGTTCCA 
GATGATCCAG AGCTTTTTGG GACTCCTGAA GATCCTTACA TAAGTCCAAT TTGTAATGCT 
GACAACAAAG AAAATAATAA ACGGAATAAT CTGAGTGCAT TAATGTGTAA CCTTCAGGAA 
CAGGAGAGCG CCCATATCAA TGAAAGCACA AATGATGCCA GCCCTGAGGA AAATGTAGAA 
GAGAGAAGTA CATATTTTGC AAAAGTAATC ACTGATCTAG AAGTTAATGA TGATCCTCTC 
ATCATACATA ACACTTTGGA AAAGAACAGT GAAGATTTTT ATGATGGTGG ACAGTTTGAT 
TTTGAGGCGT TCAGAGATCT TGACGCAGAT AAAGAGATTA AATATGCTGA AAAGAATTCA 
TCTGAAAGCG GAAGCAGTTT TGAAGAGTCC TTGTCCAAGG ATACTTCTCC GGGGTCCTCT 
CATTCCAATA GCCGTCGTTG GAGAAGAGAT CCCTCACATA CCTATAAGCC TATGCATTGG 
ATGAATGATT TCAGATACTC TGGAAAATGC ATGGGATTGA CGGATGTGAT TACTTTGGAT 
CAGAAAGAAC AGAGGAATAT TGATAATCTC TTTTACGATT TTACACCTAT ACAGAAGACC 
TTTTTGCCCA TTAGATACTG CAAATATTTT TTTAATACTT TCCGTGGATG CATTAAACCA 
GATTGTATGT ACCAGCACGT ACCATTCCAA AGGGATGAAA AGGTGTGCAT GGAGGTGATT 
CACAAACTTG TGAATGAAAA TCACACAACA TTATTAAAAC GTGCTGTTTG GATATTTACT 
GCCTACTACA GAATGTATAT GCCTGGTGTT CATTATGACT CAAATCTGCT GACCAAAATG 
CTCCGTGCCT TGTATGTTCG TCAGATGTGG GGAGATGTAT TTCAGTTGTT GGAAACTGGT 
GCCAATGTCA AAATATTGCC CTCCTCTGAA ATGCTTATCA GGTTATTTGA AAATATTGGC 
TCTACGGGAT TAACAGCAGC TGTACCTAGT TTAGTGGATG TATTCTGCAA GCTTGTGGAG 
GCTGGAATGA TGATGAAGCC AGAGCAAATC AACATGTTAA TAACAACACT GAATAATCTC 
CAGGCAACAA AAAATTATAT CAGTGTCATT TTAGACATAA AAACAAGAAT TGAAATGCAG 
CTTTCAGAAA AGAACTGGCT GTGTAACTTG GATATAGCAG TTGCAGAAGT TGGGCATTGT 
AAAGAAAAAA ATGATTGGAA GAAACTAGGA ACATTATATC TTAATCTGCG TACAGGGTGT 
GAGAATGTGA CTGATCTTAA GAAGTTTTCT AATTGCATTG TGGGGGCGCT TCAGAAAGTG 
CCAAGGAATG ATAAATCTGA AATTCCATAT TGTGACTTTG CAGACACAGT GTACAAAGAC 
GCCCAACTAA GTGAAATTGA CAAAAACATT TTGGGAAGGA TTGGAATATC CATCATGTAT 
CACTATTACA GAAATAAACA ATCGCAAAAG GGAAAGAGGG TGCTCAGAAA ACTTCAGGAA 
ATGCAGATTA ATTTCACAGT CCTAAAAGGA CTCACAGGGG AAGAGAGTAA AGCTACGCGC 
TGTCAAGTTG TGAACACAGC TGTAGAGATC TTCTTGAACT GTGAATACTT AAATGGTGCA 
CTGGGGGTTC TACGAGAGTC AGAGTGGATC ATTAACACAC AAGTGTGGCC ATGTGAAAGA 
ATGGATGTGC TGAAACGACA TAACCTGTTG TGTACAATTG CACAGAAAAC ACTGAATAAA 
AACATGTTTG ATGTGTGTCT TGAGGTCCTT CAGAATCTGC CAGGACTGCA GTGCTCATTA 
ACGGATGTGG ACGTCTCTCA GTACAGCTTG CTTTTCAACA AACTTTTGTC ATCCTGCATA 
GAAAATAATA CACTTGGTGT ATCCTCAACT GTGATAGATT TTATGATTGC AAAAAAAATA 
CCACTTGACT TTTTCTTACT GAGAGCACTA ATCACATCCT TGGGACGAAG CTGTCTATGG 
CTCAGAGCAA GAGAACTTTA TAAATGCGCT GTTTTGTTGG GTTGCTACCC GCCCATGGAA 
GGAAATCTGT ATCGTAAAGT GCTCTTCATC CCCTCATTCC TTACTGAGAT AGAAATGCTG 
TTATCCATTG AAATTTTCAT GGTGTCAAAT GCCAGTAGTA TTCAGTCACC AGGAGGCAGC 
CATCAGACTC TGCAAATCAT TCTCAAAAGA TGTGAAGAAG AGAGAGTTCC GAATAAAGAT 
TATCAAAGAG GTAAAGCTAG CTATCAAGAT GCAGTGGACC GGCTGATTCA GGCCTCTAGG 
CTATCAACAC CAAGATTATT CATAAAGCAC CTGACTGTCA ATAATGCCAA TGAACAGGTT 
TATATCCTTG ATTACGGCAG CTCTCTCAAA TGGTTGCATG AAAACATGAA ATGGGCTGGG 
AAAGTATGGC TTTTTCAGGG GAAAATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

topaz1 ( XM_018094576.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017950065.1 Xenopus tropicalis testis and ovary specific PAZ domain containing 1 (topaz1), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OXa44876
Clone ID Related Accession (Same CDS sequence)	XM_018094576.1
Accession Version	XM_018094576.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2457bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	testis- and ovary-specific PAZ domain-containing protein 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCTTTA TTAATGAGTG TAATGCAGGT GTGAATCAAC CCTACGTGGA CAGCAGAAAT 
GCTGTGGATT CCCTTGTGTT TCAGAGCATA AGAGGAAAAA GGAGAGTGCA CAGCAGAAAA 
CTTTATGTTC TACACACAAC TGTTATTTCA CTGTTACAGA TCCAGCACAC ACGCACAAAT 
GATGCCAGCC CTGAGGAAAA TGTAGAAGAG AGAAGTACAT ATTTTGCAAA AGTAATCACT 
GATCTAGAAG TTAATGATGA TCCTCTCATC ATACATAACA CTTTGGAAAA GAACAGTGAA 
GATTTTTATG ATGGTGGACA GTTTGATTTT GAGGCGTTCA GAGATCTTGA CGCAGATAAA 
GAGATTAAAT ATGCTGAAAA GAATTCATCT GAAAGCGGAA GCAGTTTTGA AGAGTCCTTG 
TCCAAGGATA CTTCTCCGGG GTCCTCTCAT TCCAATAGCC GTCGTTGGAG AAGAGATCCC 
TCACATACCT ATAAGCCTAT GCATTGGATG AATGATTTCA GATACTCTGG AAAATGCATG 
GGATTGACGG ATGTGATTAC TTTGGATCAG AAAGAACAGA GGAATATTGA TAATCTCTTT 
TACGATTTTA CACCTATACA GAAGACCTTT TTGCCCATTA GATACTGCAA ATATTTTTTT 
AATACTTTCC GTGGATGCAT TAAACCAGAT TGTATGTACC AGCACGTACC ATTCCAAAGG 
GATGAAAAGG TGTGCATGGA GGTGATTCAC AAACTTGTGA ATGAAAATCA CACAACATTA 
TTAAAACGTG CTGTTTGGAT ATTTACTGCC TACTACAGAA TGTATATGCC TGGTGTTCAT 
TATGACTCAA ATCTGCTGAC CAAAATGCTC CGTGCCTTGT ATGTTCGTCA GATGTGGGGA 
GATGTATTTC AGTTGTTGGA AACTGGTGCC AATGTCAAAA TATTGCCCTC CTCTGAAATG 
CTTATCAGGT TATTTGAAAA TATTGGCTCT ACGGGATTAA CAGCAGCTGT ACCTAGTTTA 
GTGGATGTAT TCTGCAAGCT TGTGGAGGCT GGAATGATGA TGAAGCCAGA GCAAATCAAC 
ATGTTAATAA CAACACTGAA TAATCTCCAG GCAACAAAAA ATTATATCAG TGTCATTTTA 
GACATAAAAA CAAGAATTGA AATGCAGCTT TCAGAAAAGA ACTGGCTGTG TAACTTGGAT 
ATAGCAGTTG CAGAAGTTGG GCATTGTAAA GAAAAAAATG ATTGGAAGAA ACTAGGAACA 
TTATATCTTA ATCTGCGTAC AGGGTGTGAG AATGTGACTG ATCTTAAGAA GTTTTCTAAT 
TGCATTGTGG GGGCGCTTCA GAAAGTGCCA AGGAATGATA AATCTGAAAT TCCATATTGT 
GACTTTGCAG ACACAGTGTA CAAAGACGCC CAACTAAGTG AAATTGACAA AAACATTTTG 
GGAAGGATTG GAATATCCAT CATGTATCAC TATTACAGAA ATAAACAATC GCAAAAGGGA 
AAGAGGGTGC TCAGAAAACT TCAGGAAATG CAGATTAATT TCACAGTCCT AAAAGGACTC 
ACAGGGGAAG AGAGTAAAGC TACGCGCTGT CAAGTTGTGA ACACAGCTGT AGAGATCTTC 
TTGAACTGTG AATACTTAAA TGGTGCACTG GGGGTTCTAC GAGAGTCAGA GTGGATCATT 
AACACACAAG TGTGGCCATG TGAAAGAATG GATGTGCTGA AACGACATAA CCTGTTGTGT 
ACAATTGCAC AGAAAACACT GAATAAAAAC ATGTTTGATG TGTGTCTTGA GGTCCTTCAG 
AATCTGCCAG GACTGCAGTG CTCATTAACG GATGTGGACG TCTCTCAGTA CAGCTTGCTT 
TTCAACAAAC TTTTGTCATC CTGCATAGAA AATAATACAC TTGGTGTATC CTCAACTGTG 
ATAGATTTTA TGATTGCAAA AAAAATACCA CTTGACTTTT TCTTACTGAG AGCACTAATC 
ACATCCTTGG GACGAAGCTG TCTATGGCTC AGAGCAAGAG AACTTTATAA ATGCGCTGTT 
TTGTTGGGTT GCTACCCGCC CATGGAAGGA AATCTGTATC GTAAAGTGCT CTTCATCCCC 
TCATTCCTTA CTGAGATAGA AATGCTGTTA TCCATTGAAA TTTTCATGGT GTCAAATGCC 
AGTAGTATTC AGTCACCAGG AGGCAGCCAT CAGACTCTGC AAATCATTCT CAAAAGATGT 
GAAGAAGAGA GAGTTCCGAA TAAAGATTAT CAAAGAGGTA AAGCTAGCTA TCAAGATGCA 
GTGGACCGGC TGATTCAGGC CTCTAGGCTA TCAACACCAA GATTATTCAT AAAGCACCTG 
ACTGTCAATA ATGCCAATGA ACAGGTTTAT ATCCTTGATT ACGGCAGCTC TCTCAAATGG 
TTGCATGAAA ACATGAAATG GGCTGGGAAA GTATGGCTTT TTCAGGGGAA AATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017950065.1
CDS	346..2802
Translation	MTFINECNAGVNQPYVDSRNAVDSLVFQSIRGKRRVHSRKLYVLHTTVISLLQIQHTRTNDASPEENVEERSTYFAKVITDLEVNDDPLIIHNTLEKNSEDFYDGGQFDFEAFRDLDADKEIKYAEKNSSESGSSFEESLSKDTSPGSSHSNSRRWRRDPSHTYKPMHWMNDFRYSGKCMGLTDVITLDQKEQRNIDNLFYDFTPIQKTFLPIRYCKYFFNTFRGCIKPDCMYQHVPFQRDEKVCMEVIHKLVNENHTTLLKRAVWIFTAYYRMYMPGVHYDSNLLTKMLRALYVRQMWGDVFQLLETGANVKILPSSEMLIRLFENIGSTGLTAAVPSLVDVFCKLVEAGMMMKPEQINMLITTLNNLQATKNYISVILDIKTRIEMQLSEKNWLCNLDIAVAEVGHCKEKNDWKKLGTLYLNLRTGCENVTDLKKFSNCIVGALQKVPRNDKSEIPYCDFADTVYKDAQLSEIDKNILGRIGISIMYHYYRNKQSQKGKRVLRKLQEMQINFTVLKGLTGEESKATRCQVVNTAVEIFLNCEYLNGALGVLRESEWIINTQVWPCERMDVLKRHNLLCTIAQKTLNKNMFDVCLEVLQNLPGLQCSLTDVDVSQYSLLFNKLLSSCIENNTLGVSSTVIDFMIAKKIPLDFFLLRALITSLGRSCLWLRARELYKCAVLLGCYPPMEGNLYRKVLFIPSFLTEIEMLLSIEIFMVSNASSIQSPGGSHQTLQIILKRCEEERVPNKDYQRGKASYQDAVDRLIQASRLSTPRLFIKHLTVNNANEQVYILDYGSSLKWLHENMKWAGKVWLFQGKI

Target ORF information:

RefSeq Version	XM_018094576.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis testis and ovary specific PAZ domain containing 1 (topaz1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018094576.1

ORF Insert Sequence:

ATGACCTTTA TTAATGAGTG TAATGCAGGT GTGAATCAAC CCTACGTGGA CAGCAGAAAT 
GCTGTGGATT CCCTTGTGTT TCAGAGCATA AGAGGAAAAA GGAGAGTGCA CAGCAGAAAA 
CTTTATGTTC TACACACAAC TGTTATTTCA CTGTTACAGA TCCAGCACAC ACGCACAAAT 
GATGCCAGCC CTGAGGAAAA TGTAGAAGAG AGAAGTACAT ATTTTGCAAA AGTAATCACT 
GATCTAGAAG TTAATGATGA TCCTCTCATC ATACATAACA CTTTGGAAAA GAACAGTGAA 
GATTTTTATG ATGGTGGACA GTTTGATTTT GAGGCGTTCA GAGATCTTGA CGCAGATAAA 
GAGATTAAAT ATGCTGAAAA GAATTCATCT GAAAGCGGAA GCAGTTTTGA AGAGTCCTTG 
TCCAAGGATA CTTCTCCGGG GTCCTCTCAT TCCAATAGCC GTCGTTGGAG AAGAGATCCC 
TCACATACCT ATAAGCCTAT GCATTGGATG AATGATTTCA GATACTCTGG AAAATGCATG 
GGATTGACGG ATGTGATTAC TTTGGATCAG AAAGAACAGA GGAATATTGA TAATCTCTTT 
TACGATTTTA CACCTATACA GAAGACCTTT TTGCCCATTA GATACTGCAA ATATTTTTTT 
AATACTTTCC GTGGATGCAT TAAACCAGAT TGTATGTACC AGCACGTACC ATTCCAAAGG 
GATGAAAAGG TGTGCATGGA GGTGATTCAC AAACTTGTGA ATGAAAATCA CACAACATTA 
TTAAAACGTG CTGTTTGGAT ATTTACTGCC TACTACAGAA TGTATATGCC TGGTGTTCAT 
TATGACTCAA ATCTGCTGAC CAAAATGCTC CGTGCCTTGT ATGTTCGTCA GATGTGGGGA 
GATGTATTTC AGTTGTTGGA AACTGGTGCC AATGTCAAAA TATTGCCCTC CTCTGAAATG 
CTTATCAGGT TATTTGAAAA TATTGGCTCT ACGGGATTAA CAGCAGCTGT ACCTAGTTTA 
GTGGATGTAT TCTGCAAGCT TGTGGAGGCT GGAATGATGA TGAAGCCAGA GCAAATCAAC 
ATGTTAATAA CAACACTGAA TAATCTCCAG GCAACAAAAA ATTATATCAG TGTCATTTTA 
GACATAAAAA CAAGAATTGA AATGCAGCTT TCAGAAAAGA ACTGGCTGTG TAACTTGGAT 
ATAGCAGTTG CAGAAGTTGG GCATTGTAAA GAAAAAAATG ATTGGAAGAA ACTAGGAACA 
TTATATCTTA ATCTGCGTAC AGGGTGTGAG AATGTGACTG ATCTTAAGAA GTTTTCTAAT 
TGCATTGTGG GGGCGCTTCA GAAAGTGCCA AGGAATGATA AATCTGAAAT TCCATATTGT 
GACTTTGCAG ACACAGTGTA CAAAGACGCC CAACTAAGTG AAATTGACAA AAACATTTTG 
GGAAGGATTG GAATATCCAT CATGTATCAC TATTACAGAA ATAAACAATC GCAAAAGGGA 
AAGAGGGTGC TCAGAAAACT TCAGGAAATG CAGATTAATT TCACAGTCCT AAAAGGACTC 
ACAGGGGAAG AGAGTAAAGC TACGCGCTGT CAAGTTGTGA ACACAGCTGT AGAGATCTTC 
TTGAACTGTG AATACTTAAA TGGTGCACTG GGGGTTCTAC GAGAGTCAGA GTGGATCATT 
AACACACAAG TGTGGCCATG TGAAAGAATG GATGTGCTGA AACGACATAA CCTGTTGTGT 
ACAATTGCAC AGAAAACACT GAATAAAAAC ATGTTTGATG TGTGTCTTGA GGTCCTTCAG 
AATCTGCCAG GACTGCAGTG CTCATTAACG GATGTGGACG TCTCTCAGTA CAGCTTGCTT 
TTCAACAAAC TTTTGTCATC CTGCATAGAA AATAATACAC TTGGTGTATC CTCAACTGTG 
ATAGATTTTA TGATTGCAAA AAAAATACCA CTTGACTTTT TCTTACTGAG AGCACTAATC 
ACATCCTTGG GACGAAGCTG TCTATGGCTC AGAGCAAGAG AACTTTATAA ATGCGCTGTT 
TTGTTGGGTT GCTACCCGCC CATGGAAGGA AATCTGTATC GTAAAGTGCT CTTCATCCCC 
TCATTCCTTA CTGAGATAGA AATGCTGTTA TCCATTGAAA TTTTCATGGT GTCAAATGCC 
AGTAGTATTC AGTCACCAGG AGGCAGCCAT CAGACTCTGC AAATCATTCT CAAAAGATGT 
GAAGAAGAGA GAGTTCCGAA TAAAGATTAT CAAAGAGGTA AAGCTAGCTA TCAAGATGCA 
GTGGACCGGC TGATTCAGGC CTCTAGGCTA TCAACACCAA GATTATTCAT AAAGCACCTG 
ACTGTCAATA ATGCCAATGA ACAGGTTTAT ATCCTTGATT ACGGCAGCTC TCTCAAATGG 
TTGCATGAAA ACATGAAATG GGCTGGGAAA GTATGGCTTT TTCAGGGGAA AATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

topaz1 ( XM_031903275.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031759135.1 Xenopus tropicalis testis and ovary specific PAZ domain containing 1 (topaz1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa59574
Clone ID Related Accession (Same CDS sequence)	XM_031903275.1 , XM_018094575.2 , XM_031903276.1
Accession Version	XM_031903275.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2490bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	protein TOPAZ1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCTACA CACAACTGTT ATTTCACTGT TACAGATCCA GCACACACGA TGTTGTTCCA 
GATGATCCAG AGCTTTTTGG GACTCCTGAA GATCCTTACA TAAGTCCAAT TTGTAATGCT 
GACAACAAAG AAAATAATAA ACGGAATAAT CTGAGTGCAT TAATGTGTAA CCTTCAGGAA 
CAGGAGAGCG CCCATATCAA TGAAAGCACA AATGATGCCA GCCCTGAGGA AAATGTAGAA 
GAGAGAAGTA CATATTTTGC AAAAGTAATC ACTGATCTAG AAGTTAATGA TGATCCTCTC 
ATCATACATA ACACTTTGGA AAAGAACAGT GAAGATTTTT ATGATGGTGG ACAGTTTGAT 
TTTGAGGCGT TCAGAGATCT TGACACAGAT AAAGAGATTA AATATGCTGA AAAGAATTCA 
TCTGAAAGCG GAAGCAGTTT TGAAGAGTCC TTGTCCAAGG ATACTTCTCC GGGGTCCTCT 
CATTCCAATA GCCGTCGTTG GAGAAGAGAT CCCTCACATA CCTATAAGCC TATGCATTGG 
ATGAATGATT TCAGATACTC TGGAAAATGC ATGGGATTGA CGGATGTGAT TACTTTGGAT 
CAGAAAGAAC AGAGGAATAT TGATAATCTC TTTTACGATT TTACACCTAT ACAGAAGACC 
TTTTTGCCCA TTAGATACTG CAAATATTTT TTTAATACTT TCCGTGGATG CATTAAACCA 
GATTGTATGT ACCAGCACGT ACCATTCCAA AGGGATGAAA AGGTGTGCAT GGAGGTGATT 
CACAAACTTG TGAATGAAAA TCACACAACA TTATTAAAAC GTGCTGTTTG GATATTTACT 
GCCTACTACA GAATGTATAT GCCTGGTGTT CATTATGACT CAAATCTGCT GACCAAAATG 
CTCCGTGCCT TGTATGTTCG TCAGATGTGG GGAGATGTAT TTCAGTTGTT GGAAACTGGT 
GCCAATGTCA AAATATTGCC CTCCTCTGAA ATGCTTATCA GGTTATTTGA AAATATTGGC 
TCTACGGGAT TAACAGCAGC TGTACCTAGT TTAGTGGATG TATTCTGCAA GCTTGTGGAG 
GCTGGAATGA TGATGAAGCC AGAGCAAATC AACATGTTAA TAACAACACT GAATAATCTC 
CAGGCAACAA AAAATTATAT CAGTGTCATT TTAGACATAA AAACAAGAAT TGAAATGCAG 
CTTTCAGAAA AGAACTGGCT GTGTAACTTG GATATAGCAG TTGCAGAAGT TGGGCATTGT 
AAAGAAAAAA ATGATTGGAA GAAACTAGGA ACATTATATC TTAATCTGCG TACAGGGTGT 
GAGAATGTGA CTGATCTTAA GAAGTTTTCT AATTGCATTG TCGGGGCGCT TCAGAAAGTG 
CCAAGGAATG ATAAATCTGA AATTCCATAT TGTGACTTTG CAGACACAGT GTACAAAGAC 
GCCCAACTAA GTGAAATTGA CAAAAACATT TTGGGAAGGA TTGGAATATC CATCATGTAT 
CACTATTACA GAAATAAACA ATCGCAAAAG GGAAAGAGGG TGCTCAGAAA ACTTCAGGAA 
ATGCAGATTA ATTTCACAGT CCTAAAAGGA CTCACAGGGG AAGAGAGTAA AGCTACGCGC 
TGTCAAGTTG TGAACACAGC TGTAGAGATC TTCTTGAACT GTGAATACTT AAATGGTGCA 
CTGGGGGTTC TACGAGAGTC AGAGTGGATC ATTAACACAC AAGTGTGGCC CTGTGAAAGA 
ATGGATGTGC TGAAACGACA TAACCTGTTG TGTACAATTG CACAGAAAAC ACTGAATAAA 
AACATGTTTG ATGTGTGTCT TGAGGTCCTT CAGAATCTGC CAGGACTGCA GTGCTCATTA 
ACGGATGTGG ATGTCTCTCA GTACAGCTTG CTTTTCAACA AACTTTTGTC ATCCTGCATA 
GAAAATAATA CACTTGGTGT ATCCTCAACT GTGATAGATT TTATGATTGC AAAAAAAATA 
CCACTTGACT TTTTCTTACT GAGAGCACTA ATCACATCCT TGGGACGAAG CTGTCTATGG 
CTCAGAGCAA GAGAACTTTA TAAATGCGCT GTTTTGTTGG GTTGCTACCC GCCCATGGAA 
GGAAATCTGT ATCGTAAAGT GCTCTTCATC CCCTCATTCC TTTCTGAGAT AGAAATGCTG 
TTATCCATTG AAATTTTCAT GGTGTCAAAT GCCAGTAGTA TTCAGTCACC AGGAGGCAGC 
CATCAGACTC TGCAAATCAT TCTCAAAAGA TGTGAAGAAG AGAGAGTTCC GAATAAAGAT 
TATCAAAGAG GTAAAGCTAG CTATCAAGAT GCAGTGGACC GGCTGATTCA GGCCTCTAGG 
CTATCAACAC CAAGATTATT CATAAAGCAC CTGACTGTCA ATAATGCCAA TGAACAGGTT 
TATATCCTTG ATTACGGCAG CTCTCTCAAA TGGTTGCATG AAAACATGAA ATGGGCTGGG 
AAAGTATGGC TTTTTCAGGG GAAAATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031759135.1
CDS	152..2641
Translation	MFYTQLLFHCYRSSTHDVVPDDPELFGTPEDPYISPICNADNKENNKRNNLSALMCNLQEQESAHINESTNDASPEENVEERSTYFAKVITDLEVNDDPLIIHNTLEKNSEDFYDGGQFDFEAFRDLDTDKEIKYAEKNSSESGSSFEESLSKDTSPGSSHSNSRRWRRDPSHTYKPMHWMNDFRYSGKCMGLTDVITLDQKEQRNIDNLFYDFTPIQKTFLPIRYCKYFFNTFRGCIKPDCMYQHVPFQRDEKVCMEVIHKLVNENHTTLLKRAVWIFTAYYRMYMPGVHYDSNLLTKMLRALYVRQMWGDVFQLLETGANVKILPSSEMLIRLFENIGSTGLTAAVPSLVDVFCKLVEAGMMMKPEQINMLITTLNNLQATKNYISVILDIKTRIEMQLSEKNWLCNLDIAVAEVGHCKEKNDWKKLGTLYLNLRTGCENVTDLKKFSNCIVGALQKVPRNDKSEIPYCDFADTVYKDAQLSEIDKNILGRIGISIMYHYYRNKQSQKGKRVLRKLQEMQINFTVLKGLTGEESKATRCQVVNTAVEIFLNCEYLNGALGVLRESEWIINTQVWPCERMDVLKRHNLLCTIAQKTLNKNMFDVCLEVLQNLPGLQCSLTDVDVSQYSLLFNKLLSSCIENNTLGVSSTVIDFMIAKKIPLDFFLLRALITSLGRSCLWLRARELYKCAVLLGCYPPMEGNLYRKVLFIPSFLSEIEMLLSIEIFMVSNASSIQSPGGSHQTLQIILKRCEEERVPNKDYQRGKASYQDAVDRLIQASRLSTPRLFIKHLTVNNANEQVYILDYGSSLKWLHENMKWAGKVWLFQGKI

Target ORF information:

RefSeq Version	XM_031903275.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis testis and ovary specific PAZ domain containing 1 (topaz1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031903275.1

ORF Insert Sequence:

ATGTTCTACA CACAACTGTT ATTTCACTGT TACAGATCCA GCACACACGA TGTTGTTCCA 
GATGATCCAG AGCTTTTTGG GACTCCTGAA GATCCTTACA TAAGTCCAAT TTGTAATGCT 
GACAACAAAG AAAATAATAA ACGGAATAAT CTGAGTGCAT TAATGTGTAA CCTTCAGGAA 
CAGGAGAGCG CCCATATCAA TGAAAGCACA AATGATGCCA GCCCTGAGGA AAATGTAGAA 
GAGAGAAGTA CATATTTTGC AAAAGTAATC ACTGATCTAG AAGTTAATGA TGATCCTCTC 
ATCATACATA ACACTTTGGA AAAGAACAGT GAAGATTTTT ATGATGGTGG ACAGTTTGAT 
TTTGAGGCGT TCAGAGATCT TGACACAGAT AAAGAGATTA AATATGCTGA AAAGAATTCA 
TCTGAAAGCG GAAGCAGTTT TGAAGAGTCC TTGTCCAAGG ATACTTCTCC GGGGTCCTCT 
CATTCCAATA GCCGTCGTTG GAGAAGAGAT CCCTCACATA CCTATAAGCC TATGCATTGG 
ATGAATGATT TCAGATACTC TGGAAAATGC ATGGGATTGA CGGATGTGAT TACTTTGGAT 
CAGAAAGAAC AGAGGAATAT TGATAATCTC TTTTACGATT TTACACCTAT ACAGAAGACC 
TTTTTGCCCA TTAGATACTG CAAATATTTT TTTAATACTT TCCGTGGATG CATTAAACCA 
GATTGTATGT ACCAGCACGT ACCATTCCAA AGGGATGAAA AGGTGTGCAT GGAGGTGATT 
CACAAACTTG TGAATGAAAA TCACACAACA TTATTAAAAC GTGCTGTTTG GATATTTACT 
GCCTACTACA GAATGTATAT GCCTGGTGTT CATTATGACT CAAATCTGCT GACCAAAATG 
CTCCGTGCCT TGTATGTTCG TCAGATGTGG GGAGATGTAT TTCAGTTGTT GGAAACTGGT 
GCCAATGTCA AAATATTGCC CTCCTCTGAA ATGCTTATCA GGTTATTTGA AAATATTGGC 
TCTACGGGAT TAACAGCAGC TGTACCTAGT TTAGTGGATG TATTCTGCAA GCTTGTGGAG 
GCTGGAATGA TGATGAAGCC AGAGCAAATC AACATGTTAA TAACAACACT GAATAATCTC 
CAGGCAACAA AAAATTATAT CAGTGTCATT TTAGACATAA AAACAAGAAT TGAAATGCAG 
CTTTCAGAAA AGAACTGGCT GTGTAACTTG GATATAGCAG TTGCAGAAGT TGGGCATTGT 
AAAGAAAAAA ATGATTGGAA GAAACTAGGA ACATTATATC TTAATCTGCG TACAGGGTGT 
GAGAATGTGA CTGATCTTAA GAAGTTTTCT AATTGCATTG TCGGGGCGCT TCAGAAAGTG 
CCAAGGAATG ATAAATCTGA AATTCCATAT TGTGACTTTG CAGACACAGT GTACAAAGAC 
GCCCAACTAA GTGAAATTGA CAAAAACATT TTGGGAAGGA TTGGAATATC CATCATGTAT 
CACTATTACA GAAATAAACA ATCGCAAAAG GGAAAGAGGG TGCTCAGAAA ACTTCAGGAA 
ATGCAGATTA ATTTCACAGT CCTAAAAGGA CTCACAGGGG AAGAGAGTAA AGCTACGCGC 
TGTCAAGTTG TGAACACAGC TGTAGAGATC TTCTTGAACT GTGAATACTT AAATGGTGCA 
CTGGGGGTTC TACGAGAGTC AGAGTGGATC ATTAACACAC AAGTGTGGCC CTGTGAAAGA 
ATGGATGTGC TGAAACGACA TAACCTGTTG TGTACAATTG CACAGAAAAC ACTGAATAAA 
AACATGTTTG ATGTGTGTCT TGAGGTCCTT CAGAATCTGC CAGGACTGCA GTGCTCATTA 
ACGGATGTGG ATGTCTCTCA GTACAGCTTG CTTTTCAACA AACTTTTGTC ATCCTGCATA 
GAAAATAATA CACTTGGTGT ATCCTCAACT GTGATAGATT TTATGATTGC AAAAAAAATA 
CCACTTGACT TTTTCTTACT GAGAGCACTA ATCACATCCT TGGGACGAAG CTGTCTATGG 
CTCAGAGCAA GAGAACTTTA TAAATGCGCT GTTTTGTTGG GTTGCTACCC GCCCATGGAA 
GGAAATCTGT ATCGTAAAGT GCTCTTCATC CCCTCATTCC TTTCTGAGAT AGAAATGCTG 
TTATCCATTG AAATTTTCAT GGTGTCAAAT GCCAGTAGTA TTCAGTCACC AGGAGGCAGC 
CATCAGACTC TGCAAATCAT TCTCAAAAGA TGTGAAGAAG AGAGAGTTCC GAATAAAGAT 
TATCAAAGAG GTAAAGCTAG CTATCAAGAT GCAGTGGACC GGCTGATTCA GGCCTCTAGG 
CTATCAACAC CAAGATTATT CATAAAGCAC CTGACTGTCA ATAATGCCAA TGAACAGGTT 
TATATCCTTG ATTACGGCAG CTCTCTCAAA TGGTTGCATG AAAACATGAA ATGGGCTGGG 
AAAGTATGGC TTTTTCAGGG GAAAATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

topaz1 ( XM_018094575.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017950064.2 Xenopus tropicalis testis and ovary specific PAZ domain containing 1 (topaz1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa59574
Clone ID Related Accession (Same CDS sequence)	XM_031903275.1 , XM_018094575.2 , XM_031903276.1
Accession Version	XM_018094575.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2490bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	protein TOPAZ1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_018094575.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCTACA CACAACTGTT ATTTCACTGT TACAGATCCA GCACACACGA TGTTGTTCCA 
GATGATCCAG AGCTTTTTGG GACTCCTGAA GATCCTTACA TAAGTCCAAT TTGTAATGCT 
GACAACAAAG AAAATAATAA ACGGAATAAT CTGAGTGCAT TAATGTGTAA CCTTCAGGAA 
CAGGAGAGCG CCCATATCAA TGAAAGCACA AATGATGCCA GCCCTGAGGA AAATGTAGAA 
GAGAGAAGTA CATATTTTGC AAAAGTAATC ACTGATCTAG AAGTTAATGA TGATCCTCTC 
ATCATACATA ACACTTTGGA AAAGAACAGT GAAGATTTTT ATGATGGTGG ACAGTTTGAT 
TTTGAGGCGT TCAGAGATCT TGACACAGAT AAAGAGATTA AATATGCTGA AAAGAATTCA 
TCTGAAAGCG GAAGCAGTTT TGAAGAGTCC TTGTCCAAGG ATACTTCTCC GGGGTCCTCT 
CATTCCAATA GCCGTCGTTG GAGAAGAGAT CCCTCACATA CCTATAAGCC TATGCATTGG 
ATGAATGATT TCAGATACTC TGGAAAATGC ATGGGATTGA CGGATGTGAT TACTTTGGAT 
CAGAAAGAAC AGAGGAATAT TGATAATCTC TTTTACGATT TTACACCTAT ACAGAAGACC 
TTTTTGCCCA TTAGATACTG CAAATATTTT TTTAATACTT TCCGTGGATG CATTAAACCA 
GATTGTATGT ACCAGCACGT ACCATTCCAA AGGGATGAAA AGGTGTGCAT GGAGGTGATT 
CACAAACTTG TGAATGAAAA TCACACAACA TTATTAAAAC GTGCTGTTTG GATATTTACT 
GCCTACTACA GAATGTATAT GCCTGGTGTT CATTATGACT CAAATCTGCT GACCAAAATG 
CTCCGTGCCT TGTATGTTCG TCAGATGTGG GGAGATGTAT TTCAGTTGTT GGAAACTGGT 
GCCAATGTCA AAATATTGCC CTCCTCTGAA ATGCTTATCA GGTTATTTGA AAATATTGGC 
TCTACGGGAT TAACAGCAGC TGTACCTAGT TTAGTGGATG TATTCTGCAA GCTTGTGGAG 
GCTGGAATGA TGATGAAGCC AGAGCAAATC AACATGTTAA TAACAACACT GAATAATCTC 
CAGGCAACAA AAAATTATAT CAGTGTCATT TTAGACATAA AAACAAGAAT TGAAATGCAG 
CTTTCAGAAA AGAACTGGCT GTGTAACTTG GATATAGCAG TTGCAGAAGT TGGGCATTGT 
AAAGAAAAAA ATGATTGGAA GAAACTAGGA ACATTATATC TTAATCTGCG TACAGGGTGT 
GAGAATGTGA CTGATCTTAA GAAGTTTTCT AATTGCATTG TCGGGGCGCT TCAGAAAGTG 
CCAAGGAATG ATAAATCTGA AATTCCATAT TGTGACTTTG CAGACACAGT GTACAAAGAC 
GCCCAACTAA GTGAAATTGA CAAAAACATT TTGGGAAGGA TTGGAATATC CATCATGTAT 
CACTATTACA GAAATAAACA ATCGCAAAAG GGAAAGAGGG TGCTCAGAAA ACTTCAGGAA 
ATGCAGATTA ATTTCACAGT CCTAAAAGGA CTCACAGGGG AAGAGAGTAA AGCTACGCGC 
TGTCAAGTTG TGAACACAGC TGTAGAGATC TTCTTGAACT GTGAATACTT AAATGGTGCA 
CTGGGGGTTC TACGAGAGTC AGAGTGGATC ATTAACACAC AAGTGTGGCC CTGTGAAAGA 
ATGGATGTGC TGAAACGACA TAACCTGTTG TGTACAATTG CACAGAAAAC ACTGAATAAA 
AACATGTTTG ATGTGTGTCT TGAGGTCCTT CAGAATCTGC CAGGACTGCA GTGCTCATTA 
ACGGATGTGG ATGTCTCTCA GTACAGCTTG CTTTTCAACA AACTTTTGTC ATCCTGCATA 
GAAAATAATA CACTTGGTGT ATCCTCAACT GTGATAGATT TTATGATTGC AAAAAAAATA 
CCACTTGACT TTTTCTTACT GAGAGCACTA ATCACATCCT TGGGACGAAG CTGTCTATGG 
CTCAGAGCAA GAGAACTTTA TAAATGCGCT GTTTTGTTGG GTTGCTACCC GCCCATGGAA 
GGAAATCTGT ATCGTAAAGT GCTCTTCATC CCCTCATTCC TTTCTGAGAT AGAAATGCTG 
TTATCCATTG AAATTTTCAT GGTGTCAAAT GCCAGTAGTA TTCAGTCACC AGGAGGCAGC 
CATCAGACTC TGCAAATCAT TCTCAAAAGA TGTGAAGAAG AGAGAGTTCC GAATAAAGAT 
TATCAAAGAG GTAAAGCTAG CTATCAAGAT GCAGTGGACC GGCTGATTCA GGCCTCTAGG 
CTATCAACAC CAAGATTATT CATAAAGCAC CTGACTGTCA ATAATGCCAA TGAACAGGTT 
TATATCCTTG ATTACGGCAG CTCTCTCAAA TGGTTGCATG AAAACATGAA ATGGGCTGGG 
AAAGTATGGC TTTTTCAGGG GAAAATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017950064.2
CDS	161..2650
Translation	MFYTQLLFHCYRSSTHDVVPDDPELFGTPEDPYISPICNADNKENNKRNNLSALMCNLQEQESAHINESTNDASPEENVEERSTYFAKVITDLEVNDDPLIIHNTLEKNSEDFYDGGQFDFEAFRDLDTDKEIKYAEKNSSESGSSFEESLSKDTSPGSSHSNSRRWRRDPSHTYKPMHWMNDFRYSGKCMGLTDVITLDQKEQRNIDNLFYDFTPIQKTFLPIRYCKYFFNTFRGCIKPDCMYQHVPFQRDEKVCMEVIHKLVNENHTTLLKRAVWIFTAYYRMYMPGVHYDSNLLTKMLRALYVRQMWGDVFQLLETGANVKILPSSEMLIRLFENIGSTGLTAAVPSLVDVFCKLVEAGMMMKPEQINMLITTLNNLQATKNYISVILDIKTRIEMQLSEKNWLCNLDIAVAEVGHCKEKNDWKKLGTLYLNLRTGCENVTDLKKFSNCIVGALQKVPRNDKSEIPYCDFADTVYKDAQLSEIDKNILGRIGISIMYHYYRNKQSQKGKRVLRKLQEMQINFTVLKGLTGEESKATRCQVVNTAVEIFLNCEYLNGALGVLRESEWIINTQVWPCERMDVLKRHNLLCTIAQKTLNKNMFDVCLEVLQNLPGLQCSLTDVDVSQYSLLFNKLLSSCIENNTLGVSSTVIDFMIAKKIPLDFFLLRALITSLGRSCLWLRARELYKCAVLLGCYPPMEGNLYRKVLFIPSFLSEIEMLLSIEIFMVSNASSIQSPGGSHQTLQIILKRCEEERVPNKDYQRGKASYQDAVDRLIQASRLSTPRLFIKHLTVNNANEQVYILDYGSSLKWLHENMKWAGKVWLFQGKI

Target ORF information:

RefSeq Version	XM_018094575.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis testis and ovary specific PAZ domain containing 1 (topaz1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018094575.2

ORF Insert Sequence:

ATGTTCTACA CACAACTGTT ATTTCACTGT TACAGATCCA GCACACACGA TGTTGTTCCA 
GATGATCCAG AGCTTTTTGG GACTCCTGAA GATCCTTACA TAAGTCCAAT TTGTAATGCT 
GACAACAAAG AAAATAATAA ACGGAATAAT CTGAGTGCAT TAATGTGTAA CCTTCAGGAA 
CAGGAGAGCG CCCATATCAA TGAAAGCACA AATGATGCCA GCCCTGAGGA AAATGTAGAA 
GAGAGAAGTA CATATTTTGC AAAAGTAATC ACTGATCTAG AAGTTAATGA TGATCCTCTC 
ATCATACATA ACACTTTGGA AAAGAACAGT GAAGATTTTT ATGATGGTGG ACAGTTTGAT 
TTTGAGGCGT TCAGAGATCT TGACACAGAT AAAGAGATTA AATATGCTGA AAAGAATTCA 
TCTGAAAGCG GAAGCAGTTT TGAAGAGTCC TTGTCCAAGG ATACTTCTCC GGGGTCCTCT 
CATTCCAATA GCCGTCGTTG GAGAAGAGAT CCCTCACATA CCTATAAGCC TATGCATTGG 
ATGAATGATT TCAGATACTC TGGAAAATGC ATGGGATTGA CGGATGTGAT TACTTTGGAT 
CAGAAAGAAC AGAGGAATAT TGATAATCTC TTTTACGATT TTACACCTAT ACAGAAGACC 
TTTTTGCCCA TTAGATACTG CAAATATTTT TTTAATACTT TCCGTGGATG CATTAAACCA 
GATTGTATGT ACCAGCACGT ACCATTCCAA AGGGATGAAA AGGTGTGCAT GGAGGTGATT 
CACAAACTTG TGAATGAAAA TCACACAACA TTATTAAAAC GTGCTGTTTG GATATTTACT 
GCCTACTACA GAATGTATAT GCCTGGTGTT CATTATGACT CAAATCTGCT GACCAAAATG 
CTCCGTGCCT TGTATGTTCG TCAGATGTGG GGAGATGTAT TTCAGTTGTT GGAAACTGGT 
GCCAATGTCA AAATATTGCC CTCCTCTGAA ATGCTTATCA GGTTATTTGA AAATATTGGC 
TCTACGGGAT TAACAGCAGC TGTACCTAGT TTAGTGGATG TATTCTGCAA GCTTGTGGAG 
GCTGGAATGA TGATGAAGCC AGAGCAAATC AACATGTTAA TAACAACACT GAATAATCTC 
CAGGCAACAA AAAATTATAT CAGTGTCATT TTAGACATAA AAACAAGAAT TGAAATGCAG 
CTTTCAGAAA AGAACTGGCT GTGTAACTTG GATATAGCAG TTGCAGAAGT TGGGCATTGT 
AAAGAAAAAA ATGATTGGAA GAAACTAGGA ACATTATATC TTAATCTGCG TACAGGGTGT 
GAGAATGTGA CTGATCTTAA GAAGTTTTCT AATTGCATTG TCGGGGCGCT TCAGAAAGTG 
CCAAGGAATG ATAAATCTGA AATTCCATAT TGTGACTTTG CAGACACAGT GTACAAAGAC 
GCCCAACTAA GTGAAATTGA CAAAAACATT TTGGGAAGGA TTGGAATATC CATCATGTAT 
CACTATTACA GAAATAAACA ATCGCAAAAG GGAAAGAGGG TGCTCAGAAA ACTTCAGGAA 
ATGCAGATTA ATTTCACAGT CCTAAAAGGA CTCACAGGGG AAGAGAGTAA AGCTACGCGC 
TGTCAAGTTG TGAACACAGC TGTAGAGATC TTCTTGAACT GTGAATACTT AAATGGTGCA 
CTGGGGGTTC TACGAGAGTC AGAGTGGATC ATTAACACAC AAGTGTGGCC CTGTGAAAGA 
ATGGATGTGC TGAAACGACA TAACCTGTTG TGTACAATTG CACAGAAAAC ACTGAATAAA 
AACATGTTTG ATGTGTGTCT TGAGGTCCTT CAGAATCTGC CAGGACTGCA GTGCTCATTA 
ACGGATGTGG ATGTCTCTCA GTACAGCTTG CTTTTCAACA AACTTTTGTC ATCCTGCATA 
GAAAATAATA CACTTGGTGT ATCCTCAACT GTGATAGATT TTATGATTGC AAAAAAAATA 
CCACTTGACT TTTTCTTACT GAGAGCACTA ATCACATCCT TGGGACGAAG CTGTCTATGG 
CTCAGAGCAA GAGAACTTTA TAAATGCGCT GTTTTGTTGG GTTGCTACCC GCCCATGGAA 
GGAAATCTGT ATCGTAAAGT GCTCTTCATC CCCTCATTCC TTTCTGAGAT AGAAATGCTG 
TTATCCATTG AAATTTTCAT GGTGTCAAAT GCCAGTAGTA TTCAGTCACC AGGAGGCAGC 
CATCAGACTC TGCAAATCAT TCTCAAAAGA TGTGAAGAAG AGAGAGTTCC GAATAAAGAT 
TATCAAAGAG GTAAAGCTAG CTATCAAGAT GCAGTGGACC GGCTGATTCA GGCCTCTAGG 
CTATCAACAC CAAGATTATT CATAAAGCAC CTGACTGTCA ATAATGCCAA TGAACAGGTT 
TATATCCTTG ATTACGGCAG CTCTCTCAAA TGGTTGCATG AAAACATGAA ATGGGCTGGG 
AAAGTATGGC TTTTTCAGGG GAAAATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

topaz1 ( XM_031903276.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031759136.1 Xenopus tropicalis testis and ovary specific PAZ domain containing 1 (topaz1), transcript variant X3, mRNA.

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CloneID	OXa59574
Clone ID Related Accession (Same CDS sequence)	XM_031903275.1 , XM_018094575.2 , XM_031903276.1
Accession Version	XM_031903276.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2490bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	protein TOPAZ1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCTACA CACAACTGTT ATTTCACTGT TACAGATCCA GCACACACGA TGTTGTTCCA 
GATGATCCAG AGCTTTTTGG GACTCCTGAA GATCCTTACA TAAGTCCAAT TTGTAATGCT 
GACAACAAAG AAAATAATAA ACGGAATAAT CTGAGTGCAT TAATGTGTAA CCTTCAGGAA 
CAGGAGAGCG CCCATATCAA TGAAAGCACA AATGATGCCA GCCCTGAGGA AAATGTAGAA 
GAGAGAAGTA CATATTTTGC AAAAGTAATC ACTGATCTAG AAGTTAATGA TGATCCTCTC 
ATCATACATA ACACTTTGGA AAAGAACAGT GAAGATTTTT ATGATGGTGG ACAGTTTGAT 
TTTGAGGCGT TCAGAGATCT TGACACAGAT AAAGAGATTA AATATGCTGA AAAGAATTCA 
TCTGAAAGCG GAAGCAGTTT TGAAGAGTCC TTGTCCAAGG ATACTTCTCC GGGGTCCTCT 
CATTCCAATA GCCGTCGTTG GAGAAGAGAT CCCTCACATA CCTATAAGCC TATGCATTGG 
ATGAATGATT TCAGATACTC TGGAAAATGC ATGGGATTGA CGGATGTGAT TACTTTGGAT 
CAGAAAGAAC AGAGGAATAT TGATAATCTC TTTTACGATT TTACACCTAT ACAGAAGACC 
TTTTTGCCCA TTAGATACTG CAAATATTTT TTTAATACTT TCCGTGGATG CATTAAACCA 
GATTGTATGT ACCAGCACGT ACCATTCCAA AGGGATGAAA AGGTGTGCAT GGAGGTGATT 
CACAAACTTG TGAATGAAAA TCACACAACA TTATTAAAAC GTGCTGTTTG GATATTTACT 
GCCTACTACA GAATGTATAT GCCTGGTGTT CATTATGACT CAAATCTGCT GACCAAAATG 
CTCCGTGCCT TGTATGTTCG TCAGATGTGG GGAGATGTAT TTCAGTTGTT GGAAACTGGT 
GCCAATGTCA AAATATTGCC CTCCTCTGAA ATGCTTATCA GGTTATTTGA AAATATTGGC 
TCTACGGGAT TAACAGCAGC TGTACCTAGT TTAGTGGATG TATTCTGCAA GCTTGTGGAG 
GCTGGAATGA TGATGAAGCC AGAGCAAATC AACATGTTAA TAACAACACT GAATAATCTC 
CAGGCAACAA AAAATTATAT CAGTGTCATT TTAGACATAA AAACAAGAAT TGAAATGCAG 
CTTTCAGAAA AGAACTGGCT GTGTAACTTG GATATAGCAG TTGCAGAAGT TGGGCATTGT 
AAAGAAAAAA ATGATTGGAA GAAACTAGGA ACATTATATC TTAATCTGCG TACAGGGTGT 
GAGAATGTGA CTGATCTTAA GAAGTTTTCT AATTGCATTG TCGGGGCGCT TCAGAAAGTG 
CCAAGGAATG ATAAATCTGA AATTCCATAT TGTGACTTTG CAGACACAGT GTACAAAGAC 
GCCCAACTAA GTGAAATTGA CAAAAACATT TTGGGAAGGA TTGGAATATC CATCATGTAT 
CACTATTACA GAAATAAACA ATCGCAAAAG GGAAAGAGGG TGCTCAGAAA ACTTCAGGAA 
ATGCAGATTA ATTTCACAGT CCTAAAAGGA CTCACAGGGG AAGAGAGTAA AGCTACGCGC 
TGTCAAGTTG TGAACACAGC TGTAGAGATC TTCTTGAACT GTGAATACTT AAATGGTGCA 
CTGGGGGTTC TACGAGAGTC AGAGTGGATC ATTAACACAC AAGTGTGGCC CTGTGAAAGA 
ATGGATGTGC TGAAACGACA TAACCTGTTG TGTACAATTG CACAGAAAAC ACTGAATAAA 
AACATGTTTG ATGTGTGTCT TGAGGTCCTT CAGAATCTGC CAGGACTGCA GTGCTCATTA 
ACGGATGTGG ATGTCTCTCA GTACAGCTTG CTTTTCAACA AACTTTTGTC ATCCTGCATA 
GAAAATAATA CACTTGGTGT ATCCTCAACT GTGATAGATT TTATGATTGC AAAAAAAATA 
CCACTTGACT TTTTCTTACT GAGAGCACTA ATCACATCCT TGGGACGAAG CTGTCTATGG 
CTCAGAGCAA GAGAACTTTA TAAATGCGCT GTTTTGTTGG GTTGCTACCC GCCCATGGAA 
GGAAATCTGT ATCGTAAAGT GCTCTTCATC CCCTCATTCC TTTCTGAGAT AGAAATGCTG 
TTATCCATTG AAATTTTCAT GGTGTCAAAT GCCAGTAGTA TTCAGTCACC AGGAGGCAGC 
CATCAGACTC TGCAAATCAT TCTCAAAAGA TGTGAAGAAG AGAGAGTTCC GAATAAAGAT 
TATCAAAGAG GTAAAGCTAG CTATCAAGAT GCAGTGGACC GGCTGATTCA GGCCTCTAGG 
CTATCAACAC CAAGATTATT CATAAAGCAC CTGACTGTCA ATAATGCCAA TGAACAGGTT 
TATATCCTTG ATTACGGCAG CTCTCTCAAA TGGTTGCATG AAAACATGAA ATGGGCTGGG 
AAAGTATGGC TTTTTCAGGG GAAAATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031759136.1
CDS	149..2638
Translation	MFYTQLLFHCYRSSTHDVVPDDPELFGTPEDPYISPICNADNKENNKRNNLSALMCNLQEQESAHINESTNDASPEENVEERSTYFAKVITDLEVNDDPLIIHNTLEKNSEDFYDGGQFDFEAFRDLDTDKEIKYAEKNSSESGSSFEESLSKDTSPGSSHSNSRRWRRDPSHTYKPMHWMNDFRYSGKCMGLTDVITLDQKEQRNIDNLFYDFTPIQKTFLPIRYCKYFFNTFRGCIKPDCMYQHVPFQRDEKVCMEVIHKLVNENHTTLLKRAVWIFTAYYRMYMPGVHYDSNLLTKMLRALYVRQMWGDVFQLLETGANVKILPSSEMLIRLFENIGSTGLTAAVPSLVDVFCKLVEAGMMMKPEQINMLITTLNNLQATKNYISVILDIKTRIEMQLSEKNWLCNLDIAVAEVGHCKEKNDWKKLGTLYLNLRTGCENVTDLKKFSNCIVGALQKVPRNDKSEIPYCDFADTVYKDAQLSEIDKNILGRIGISIMYHYYRNKQSQKGKRVLRKLQEMQINFTVLKGLTGEESKATRCQVVNTAVEIFLNCEYLNGALGVLRESEWIINTQVWPCERMDVLKRHNLLCTIAQKTLNKNMFDVCLEVLQNLPGLQCSLTDVDVSQYSLLFNKLLSSCIENNTLGVSSTVIDFMIAKKIPLDFFLLRALITSLGRSCLWLRARELYKCAVLLGCYPPMEGNLYRKVLFIPSFLSEIEMLLSIEIFMVSNASSIQSPGGSHQTLQIILKRCEEERVPNKDYQRGKASYQDAVDRLIQASRLSTPRLFIKHLTVNNANEQVYILDYGSSLKWLHENMKWAGKVWLFQGKI

Target ORF information:

RefSeq Version	XM_031903276.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis testis and ovary specific PAZ domain containing 1 (topaz1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031903276.1

ORF Insert Sequence:

ATGTTCTACA CACAACTGTT ATTTCACTGT TACAGATCCA GCACACACGA TGTTGTTCCA 
GATGATCCAG AGCTTTTTGG GACTCCTGAA GATCCTTACA TAAGTCCAAT TTGTAATGCT 
GACAACAAAG AAAATAATAA ACGGAATAAT CTGAGTGCAT TAATGTGTAA CCTTCAGGAA 
CAGGAGAGCG CCCATATCAA TGAAAGCACA AATGATGCCA GCCCTGAGGA AAATGTAGAA 
GAGAGAAGTA CATATTTTGC AAAAGTAATC ACTGATCTAG AAGTTAATGA TGATCCTCTC 
ATCATACATA ACACTTTGGA AAAGAACAGT GAAGATTTTT ATGATGGTGG ACAGTTTGAT 
TTTGAGGCGT TCAGAGATCT TGACACAGAT AAAGAGATTA AATATGCTGA AAAGAATTCA 
TCTGAAAGCG GAAGCAGTTT TGAAGAGTCC TTGTCCAAGG ATACTTCTCC GGGGTCCTCT 
CATTCCAATA GCCGTCGTTG GAGAAGAGAT CCCTCACATA CCTATAAGCC TATGCATTGG 
ATGAATGATT TCAGATACTC TGGAAAATGC ATGGGATTGA CGGATGTGAT TACTTTGGAT 
CAGAAAGAAC AGAGGAATAT TGATAATCTC TTTTACGATT TTACACCTAT ACAGAAGACC 
TTTTTGCCCA TTAGATACTG CAAATATTTT TTTAATACTT TCCGTGGATG CATTAAACCA 
GATTGTATGT ACCAGCACGT ACCATTCCAA AGGGATGAAA AGGTGTGCAT GGAGGTGATT 
CACAAACTTG TGAATGAAAA TCACACAACA TTATTAAAAC GTGCTGTTTG GATATTTACT 
GCCTACTACA GAATGTATAT GCCTGGTGTT CATTATGACT CAAATCTGCT GACCAAAATG 
CTCCGTGCCT TGTATGTTCG TCAGATGTGG GGAGATGTAT TTCAGTTGTT GGAAACTGGT 
GCCAATGTCA AAATATTGCC CTCCTCTGAA ATGCTTATCA GGTTATTTGA AAATATTGGC 
TCTACGGGAT TAACAGCAGC TGTACCTAGT TTAGTGGATG TATTCTGCAA GCTTGTGGAG 
GCTGGAATGA TGATGAAGCC AGAGCAAATC AACATGTTAA TAACAACACT GAATAATCTC 
CAGGCAACAA AAAATTATAT CAGTGTCATT TTAGACATAA AAACAAGAAT TGAAATGCAG 
CTTTCAGAAA AGAACTGGCT GTGTAACTTG GATATAGCAG TTGCAGAAGT TGGGCATTGT 
AAAGAAAAAA ATGATTGGAA GAAACTAGGA ACATTATATC TTAATCTGCG TACAGGGTGT 
GAGAATGTGA CTGATCTTAA GAAGTTTTCT AATTGCATTG TCGGGGCGCT TCAGAAAGTG 
CCAAGGAATG ATAAATCTGA AATTCCATAT TGTGACTTTG CAGACACAGT GTACAAAGAC 
GCCCAACTAA GTGAAATTGA CAAAAACATT TTGGGAAGGA TTGGAATATC CATCATGTAT 
CACTATTACA GAAATAAACA ATCGCAAAAG GGAAAGAGGG TGCTCAGAAA ACTTCAGGAA 
ATGCAGATTA ATTTCACAGT CCTAAAAGGA CTCACAGGGG AAGAGAGTAA AGCTACGCGC 
TGTCAAGTTG TGAACACAGC TGTAGAGATC TTCTTGAACT GTGAATACTT AAATGGTGCA 
CTGGGGGTTC TACGAGAGTC AGAGTGGATC ATTAACACAC AAGTGTGGCC CTGTGAAAGA 
ATGGATGTGC TGAAACGACA TAACCTGTTG TGTACAATTG CACAGAAAAC ACTGAATAAA 
AACATGTTTG ATGTGTGTCT TGAGGTCCTT CAGAATCTGC CAGGACTGCA GTGCTCATTA 
ACGGATGTGG ATGTCTCTCA GTACAGCTTG CTTTTCAACA AACTTTTGTC ATCCTGCATA 
GAAAATAATA CACTTGGTGT ATCCTCAACT GTGATAGATT TTATGATTGC AAAAAAAATA 
CCACTTGACT TTTTCTTACT GAGAGCACTA ATCACATCCT TGGGACGAAG CTGTCTATGG 
CTCAGAGCAA GAGAACTTTA TAAATGCGCT GTTTTGTTGG GTTGCTACCC GCCCATGGAA 
GGAAATCTGT ATCGTAAAGT GCTCTTCATC CCCTCATTCC TTTCTGAGAT AGAAATGCTG 
TTATCCATTG AAATTTTCAT GGTGTCAAAT GCCAGTAGTA TTCAGTCACC AGGAGGCAGC 
CATCAGACTC TGCAAATCAT TCTCAAAAGA TGTGAAGAAG AGAGAGTTCC GAATAAAGAT 
TATCAAAGAG GTAAAGCTAG CTATCAAGAT GCAGTGGACC GGCTGATTCA GGCCTCTAGG 
CTATCAACAC CAAGATTATT CATAAAGCAC CTGACTGTCA ATAATGCCAA TGAACAGGTT 
TATATCCTTG ATTACGGCAG CTCTCTCAAA TGGTTGCATG AAAACATGAA ATGGGCTGGG 
AAAGTATGGC TTTTTCAGGG GAAAATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

topaz1 ( NM_001374744.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> NP_001361673.1 Xenopus tropicalis testis and ovary specific PAZ domain containing 1 (topaz1), mRNA.

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CloneID	OXa33111
Clone ID Related Accession (Same CDS sequence)	XM_012964409.2 , NM_001374744.1
Accession Version	NM_001374744.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5691bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-09
Organism	Xenopus tropicalis(tropical clawed frog)
Product	testis- and ovary-specific PAZ domain-containing protein 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AAMC03023896.1. On Oct 10, 2019 this sequence version replaced XM_012964409.2. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN00862000, SAMN04027313 [ECO:0000348] ##Evidence-Data-END##

ATGAGTATGT TGCACTCTAG AAAAATTAAG CTAGTTCAAC CTAACCGGTC TGAATCCAGC 
TCATTTGCTA AGATCTGTGA TGAGGCTGAT ACTGAAAAAA GTAGTTCAGT TCTTATCAGT 
CCAGCTCTCT CTCTACAGTA TTCCTCAAAA GCCATTGATC AGGATAGGAG TGTAAAGAAT 
CTGTCAGGTA GAAACCCAAA GGCCATAAGT GGTGTGAATG AGCCTTGTGC AATCCAGCAA 
TGTGGAAATA TAGCGGTGTC TACAGAATCT GACTTTATTA GAATGAATGA CACAAGGAAG 
GCAAGACTAG CACTTTCAGA TCCAGTTCTT GCTTCTAATG ACTCTGGAGA AAGTATTAAT 
GTTTCAAACT TGCCGTCTTG TCAAGTTAAA AGAAGAACCA GAAGGAAGGC AACTGATCTG 
CGCGGTGCGG AGTCAATTGA AAAAGAAAAG TGTGGAAGTT TAGTAGTAAA TCAGGAAGCA 
GCAATTGTGA ATACTGATAC TACTGTTCCA CTAGATATTG AGAATGTTTA CAGTCCTACT 
CATATGCTAA ATCAACGGGG ATTGTTGAAA AAAATAGGAA AGAGGCCAAC AATTAAGAAG 
GAAGATGTGA GTAGTGTAGG AGATAGACAA AGATGTTTAA AACTAGCAGG AAAGCAAAGT 
GGAAAGAAAC TGCAATCTGA GCAAAGTGTT GTGTGTGGCA AGGGAAAGAC TCAGGAATGT 
GTACAAATAA TAGGGAAAAA AAGAGGAAGG AGGGCAAAAA CTGATCAGAG TAGTGAGTGT 
GGCATAAGTG AGATTCAGCA AAGAAAGAAA AGTAGAAAAA AGGTAAAAAC TGATAAGAGT 
GATGTGTGTT GTTCAGGAGA GACATTGAAA TATGTTAATA AAATTAAAAG AGGGAGGAAA 
CTAAAAACTG ATCATAAAGT TGTAAGTAGC AAAGGAGAAA CACAGGAATG TGTAAAATTA 
ACAGACATGA ATCAGAAACC AAAAACTGAA TGTTTTGCTG TGTGTGGCAC AGAAGAGACT 
CAGGCTTGTG TAAAACCACT GGGAAAGAAA AAAGGCAGGA TACTAGGTAA TAGTGTTGGT 
AAAAATAAAA TTAATGCTGG GTGTTATTCA GAATTAGATA AAATGCGTAC CGTATATGTT 
AGGGTACAGC GATTGTCATT GAATAAAATT CCCCAGAGTG CAGGAATCTC CACAGAACAT 
CTGAGGAGTG TGCTGCTTCT GAAGTCTGAG TATAGACAGG AATGTGGGGA AGCAAGAACA 
GAAGAGAGGA ATGATGCTAC AGATGTGACC TTCTTAAAAA AGAATCTCAG GAGTCCTAGA 
CAGCAGAAAT GCTGTGGATT CCCTTGTGTT TCAGAGCATA AGAGGAAAAA GGAGAGTGCA 
CAGCAGAAAA CTTTATGTTC TACACACAAC TGTTATTTCA CTGTTACAGA TCCAGCACAC 
ACGGTAAATG GAATAACGTG CATGCAAAAT CCATTGCTTG AAACAGCAAA CAATAGAAAA 
AGTAAATGTT CATATGGTGG TTCCCAGTCT TTTCTAAATC CTGTAGTGAA ATTGCATAAC 
TGCAGCAACA TGAAGAGGCT TATACGTTGC TCAAATCTGA GGGTAAGCAG TTCCAAAACT 
GAGAATACAT GTCCTGCAGC AACAGTAAAA GAACTTGATC CTCAAAAACA ATTACCTTGT 
TCTGTTAACA CTGTAAATTC AGTTATCAGT ACAGATTCTA CTGTTGGAGG TAATCATTCC 
AAAGAACTGG AATCTATTGC ACATGGCTTG GCAAATAGGA AAATTAAAAG AATATTAAAA 
GTAAAGTGGG ACTTTGGAAA AAAAATAAGG AATTCTGCAA CATGCTCTAC AGAGCGTACT 
CAAGAAGACT CTGCAATATC TGTCAAACAG AAAACAGGAG ACCTAAAATA TAGTGGTAAT 
CCTACTGTCA ACACAGACTC ATCAGATCCT TGCATTGGTA CTTTGGAAAA GATTATTGAC 
GAGATGAACA ATAATAAAAG TGACTCTGTA AATTTTAACT TGAAGGATGC CCTTTGTGAT 
CAAAGCACAA GTCAAAACAA AAAGGAAAGT TATGATTGCC TTCCGGTACA ATGCAGGACT 
ACACAATTAC CGTTTATGGT TTCAGAGTGT GAAAGAAAGG ATAGGGAATC CCTTGACATA 
GAGTGTTCTG CACTTATTAG AATGTCTGCA AATGAGTCAG TGGTCGATGA CTATTCAGAT 
TTTTCACTTA CTGAGGAAAA TGTTAACTTT GTAAATAATA ACAATATAAA TGAGCATACA 
GAAGAATGCA AAACCTGTTC CTGTCAGAGA ACAATCCCAT TTACTGGGAA AACCATCTGG 
AAATGTTCTT GTGCAAGGAC GTGTGTATGG GCTTTCCCAA GAAAAAGAAC TTCCTCTTTA 
CCAAGTACAG ATATACTGGG ATTTGACTCT GAATTGTCTT TTGCCCCTTA TTCCACCAAT 
ATAAAGTCTT TATTTCAGGC TAATGAAGCA GAGCCATTGC AGAGCACAAC TGATGACACT 
GACAGTGACG GGATATGTAA AAAACTAAAG TTAGATTTCA TTTCAGAAAA TGACGCTCTT 
ATTCTTAAAG ATATTGATGA TGACATTTTG TCTCACCCTC CAACAGAAAC TTTTGAACTA 
CCCAATACTG TAATTGAGAA GATATGTAAT ATAACTGAAA ATGTTTACTC ACTAGGTGAA 
GTTGAAGACA TGTATAAAAA TTTAGATGGT TGGCCATGCA GTTCAACTAT TATTCAAAAT 
ACTATAAATA GTCTTCCTCT CCCGCTGCCA AACAATGACA AAAGTGAGCA CTTTACCACT 
TCTGGCACTT ATTCTGAAAT AGAACCTACT GTAAAAACAG TTGAGGAGAT GGCCCTGAGC 
AGTTCTTTTG AAAACTCTGA TCCAAGTGTT TGTTCCAGAG ACAGCTCCAC AGAACCTGAA 
AACAGTTCAG AGACTGTGCT AAAAAGTACT CAATATGAAT CTCCAATACG TGATTGTTTA 
GACAAGCATA AAAAAAACAC TAATGTTGAG GAAGTGTTAT CCTTTTTTGA TTGTTTACAT 
GAAACTACAA TGGATAACGC CTGTCTAGCT GAGGAAAATT GCACCAGACA GATTAATAAT 
ACTGGTGGCC CATTAGATGA TTCACATTTG ACACTAAGCC ATGTAGAATC TCCAAAGAAA 
AGCAAGCTTG TGGAATACAA AAGTAATTTA GATGTGATAA AAGCTTATGA AGAAGATGTA 
CTGTTTTTAG ATGTTGTTCC AGATGATCCA GAGCTTTTTG GGACTCCTGA AGATCCTTAC 
ATAAGTCCAA TTTGTAATGC TGACAACAAA GAAAATAATA AACGGAATAA TCTGAGTGCA 
TTAATGTGTA ACCTTCAGGA ACAGGAGAGC GCCCATATCA ATGAAAGCAC AAATGATGCC 
AGCCCTGAGG AAAATGTAGA AGAGAGAAGT ACATATTTTG CAAAAGTAAT CACTGATCTA 
GAAGTTAATG ATGATCCTCT CATCATACAT AACACTTTGG AAAAGAACAG TGAAGATTTT 
TATGATGGTG GACAGTTTGA TTTTGAGGCG TTCAGAGATC TTGACGCAGA TAAAGAGATT 
AAATATGCTG AAAAGAATTC ATCTGAAAGC GGAAGCAGTT TTGAAGAGTC CTTGTCCAAG 
GATACTTCTC CGGGGTCCTC TCATTCCAAT AGCCGTCGTT GGAGAAGAGA TCCCTCACAT 
ACCTATAAGC CTATGCATTG GATGAATGAT TTCAGATACT CTGGAAAATG CATGGGATTG 
ACGGATGTGA TTACTTTGGA TCAGAAAGAA CAGAGGAATA TTGATAATCT CTTTTACGAT 
TTTACACCTA TACAGAAGAC CTTTTTGCCC ATTAGATACT GCAAATATTT TTTTAATACT 
TTCCGTGGAT GCATTAAACC AGATTGTATG TACCAGCACG TACCATTCCA AAGGGATGAA 
AAGGTGTGCA TGGAGGTGAT TCACAAACTT GTGAATGAAA ATCACACAAC ATTATTAAAA 
CGTGCTGTTT GGATATTTAC TGCCTACTAC AGAATGTATA TGCCTGGTGT TCATTATGAC 
TCAAATCTGC TGACCAAAAT GCTCCGTGCC TTGTATGTTC GTCAGATGTG GGGAGATGTA 
TTTCAGTTGT TGGAAACTGG TGCCAATGTC AAAATATTGC CCTCCTCTGA AATGCTTATC 
AGGTTATTTG AAAATATTGG CTCTACGGGA TTAACAGCAG CTGTACCTAG TTTAGTGGAT 
GTATTCTGCA AGCTTGTGGA GGCTGGAATG ATGATGAAGC CAGAGCAAAT CAACATGTTA 
ATAACAACAC TGAATAATCT CCAGGCAACA AAAAATTATA TCAGTGTCAT TTTAGACATA 
AAAACAAGAA TTGAAATGCA GCTTTCAGAA AAGAACTGGC TGTGTAACTT GGATATAGCA 
GTTGCAGAAG TTGGGCATTG TAAAGAAAAA AATGATTGGA AGAAACTAGG AACATTATAT 
CTTAATCTGC GTACAGGGTG TGAGAATGTG ACTGATCTTA AGAAGTTTTC TAATTGCATT 
GTGGGGGCGC TTCAGAAAGT GCCAAGGAAT GATAAATCTG AAATTCCATA TTGTGACTTT 
GCAGACACAG TGTACAAAGA CGCCCAACTA AGTGAAATTG ACAAAAACAT TTTGGGAAGG 
ATTGGAATAT CCATCATGTA TCACTATTAC AGAAATAAAC AATCGCAAAA GGGAAAGAGG 
GTGCTCAGAA AACTTCAGGA AATGCAGATT AATTTCACAG TCCTAAAAGG ACTCACAGGG 
GAAGAGAGTA AAGCTACGCG CTGTCAAGTT GTGAACACAG CTGTAGAGAT CTTCTTGAAC 
TGTGAATACT TAAATGGTGC ACTGGGGGTT CTACGAGAGT CAGAGTGGAT CATTAACACA 
CAAGTGTGGC CATGTGAAAG AATGGATGTG CTGAAACGAC ATAACCTGTT GTGTACAATT 
GCACAGAAAA CACTGAATAA AAACATGTTT GATGTGTGTC TTGAGGTCCT TCAGAATCTG 
CCAGGACTGC AGTGCTCATT AACGGATGTG GACGTCTCTC AGTACAGCTT GCTTTTCAAC 
AAACTTTTGT CATCCTGCAT AGAAAATAAT ACACTTGGTG TATCCTCAAC TGTGATAGAT 
TTTATGATTG CAAAAAAAAT ACCACTTGAC TTTTTCTTAC TGAGAGCACT AATCACATCC 
TTGGGACGAA GCTGTCTATG GCTCAGAGCA AGAGAACTTT ATAAATGCGC TGTTTTGTTG 
GGTTGCTACC CGCCCATGGA AGGAAATCTG TATCGTAAAG TGCTCTTCAT CCCCTCATTC 
CTTACTGAGA TAGAAATGCT GTTATCCATT GAAATTTTCA TGGTGTCAAA TGCCAGTAGT 
ATTCAGTCAC CAGGAGGCAG CCATCAGACT CTGCAAATCA TTCTCAAAAG ATGTGAAGAA 
GAGAGAGTTC CGAATAAAGA TTATCAAAGA GGTAAAGCTA GCTATCAAGA TGCAGTGGAC 
CGGCTGATTC AGGCCTCTAG GCTATCAACA CCAAGATTAT TCATAAAGCA CCTGACTGTC 
AATAATGCCA ATGAACAGGT TTATATCCTT GATTACGGCA GCTCTCTCAA ATGGTTGCAT 
GAAAACATGA AATGGGCTGG GAAAGTATGG CTTTTTCAGG GGAAAATATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001361673.1
CDS	777..6467
Translation	MSMLHSRKIKLVQPNRSESSSFAKICDEADTEKSSSVLISPALSLQYSSKAIDQDRSVKNLSGRNPKAISGVNEPCAIQQCGNIAVSTESDFIRMNDTRKARLALSDPVLASNDSGESINVSNLPSCQVKRRTRRKATDLRGAESIEKEKCGSLVVNQEAAIVNTDTTVPLDIENVYSPTHMLNQRGLLKKIGKRPTIKKEDVSSVGDRQRCLKLAGKQSGKKLQSEQSVVCGKGKTQECVQIIGKKRGRRAKTDQSSECGISEIQQRKKSRKKVKTDKSDVCCSGETLKYVNKIKRGRKLKTDHKVVSSKGETQECVKLTDMNQKPKTECFAVCGTEETQACVKPLGKKKGRILGNSVGKNKINAGCYSELDKMRTVYVRVQRLSLNKIPQSAGISTEHLRSVLLLKSEYRQECGEARTEERNDATDVTFLKKNLRSPRQQKCCGFPCVSEHKRKKESAQQKTLCSTHNCYFTVTDPAHTVNGITCMQNPLLETANNRKSKCSYGGSQSFLNPVVKLHNCSNMKRLIRCSNLRVSSSKTENTCPAATVKELDPQKQLPCSVNTVNSVISTDSTVGGNHSKELESIAHGLANRKIKRILKVKWDFGKKIRNSATCSTERTQEDSAISVKQKTGDLKYSGNPTVNTDSSDPCIGTLEKIIDEMNNNKSDSVNFNLKDALCDQSTSQNKKESYDCLPVQCRTTQLPFMVSECERKDRESLDIECSALIRMSANESVVDDYSDFSLTEENVNFVNNNNINEHTEECKTCSCQRTIPFTGKTIWKCSCARTCVWAFPRKRTSSLPSTDILGFDSELSFAPYSTNIKSLFQANEAEPLQSTTDDTDSDGICKKLKLDFISENDALILKDIDDDILSHPPTETFELPNTVIEKICNITENVYSLGEVEDMYKNLDGWPCSSTIIQNTINSLPLPLPNNDKSEHFTTSGTYSEIEPTVKTVEEMALSSSFENSDPSVCSRDSSTEPENSSETVLKSTQYESPIRDCLDKHKKNTNVEEVLSFFDCLHETTMDNACLAEENCTRQINNTGGPLDDSHLTLSHVESPKKSKLVEYKSNLDVIKAYEEDVLFLDVVPDDPELFGTPEDPYISPICNADNKENNKRNNLSALMCNLQEQESAHINESTNDASPEENVEERSTYFAKVITDLEVNDDPLIIHNTLEKNSEDFYDGGQFDFEAFRDLDADKEIKYAEKNSSESGSSFEESLSKDTSPGSSHSNSRRWRRDPSHTYKPMHWMNDFRYSGKCMGLTDVITLDQKEQRNIDNLFYDFTPIQKTFLPIRYCKYFFNTFRGCIKPDCMYQHVPFQRDEKVCMEVIHKLVNENHTTLLKRAVWIFTAYYRMYMPGVHYDSNLLTKMLRALYVRQMWGDVFQLLETGANVKILPSSEMLIRLFENIGSTGLTAAVPSLVDVFCKLVEAGMMMKPEQINMLITTLNNLQATKNYISVILDIKTRIEMQLSEKNWLCNLDIAVAEVGHCKEKNDWKKLGTLYLNLRTGCENVTDLKKFSNCIVGALQKVPRNDKSEIPYCDFADTVYKDAQLSEIDKNILGRIGISIMYHYYRNKQSQKGKRVLRKLQEMQINFTVLKGLTGEESKATRCQVVNTAVEIFLNCEYLNGALGVLRESEWIINTQVWPCERMDVLKRHNLLCTIAQKTLNKNMFDVCLEVLQNLPGLQCSLTDVDVSQYSLLFNKLLSSCIENNTLGVSSTVIDFMIAKKIPLDFFLLRALITSLGRSCLWLRARELYKCAVLLGCYPPMEGNLYRKVLFIPSFLTEIEMLLSIEIFMVSNASSIQSPGGSHQTLQIILKRCEEERVPNKDYQRGKASYQDAVDRLIQASRLSTPRLFIKHLTVNNANEQVYILDYGSSLKWLHENMKWAGKVWLFQGKI

Target ORF information:

RefSeq Version	NM_001374744.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis testis and ovary specific PAZ domain containing 1 (topaz1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001374744.1

ORF Insert Sequence:

ATGAGTATGT TGCACTCTAG AAAAATTAAG CTAGTTCAAC CTAACCGGTC TGAATCCAGC 
TCATTTGCTA AGATCTGTGA TGAGGCTGAT ACTGAAAAAA GTAGTTCAGT TCTTATCAGT 
CCAGCTCTCT CTCTACAGTA TTCCTCAAAA GCCATTGATC AGGATAGGAG TGTAAAGAAT 
CTGTCAGGTA GAAACCCAAA GGCCATAAGT GGTGTGAATG AGCCTTGTGC AATCCAGCAA 
TGTGGAAATA TAGCGGTGTC TACAGAATCT GACTTTATTA GAATGAATGA CACAAGGAAG 
GCAAGACTAG CACTTTCAGA TCCAGTTCTT GCTTCTAATG ACTCTGGAGA AAGTATTAAT 
GTTTCAAACT TGCCGTCTTG TCAAGTTAAA AGAAGAACCA GAAGGAAGGC AACTGATCTG 
CGCGGTGCGG AGTCAATTGA AAAAGAAAAG TGTGGAAGTT TAGTAGTAAA TCAGGAAGCA 
GCAATTGTGA ATACTGATAC TACTGTTCCA CTAGATATTG AGAATGTTTA CAGTCCTACT 
CATATGCTAA ATCAACGGGG ATTGTTGAAA AAAATAGGAA AGAGGCCAAC AATTAAGAAG 
GAAGATGTGA GTAGTGTAGG AGATAGACAA AGATGTTTAA AACTAGCAGG AAAGCAAAGT 
GGAAAGAAAC TGCAATCTGA GCAAAGTGTT GTGTGTGGCA AGGGAAAGAC TCAGGAATGT 
GTACAAATAA TAGGGAAAAA AAGAGGAAGG AGGGCAAAAA CTGATCAGAG TAGTGAGTGT 
GGCATAAGTG AGATTCAGCA AAGAAAGAAA AGTAGAAAAA AGGTAAAAAC TGATAAGAGT 
GATGTGTGTT GTTCAGGAGA GACATTGAAA TATGTTAATA AAATTAAAAG AGGGAGGAAA 
CTAAAAACTG ATCATAAAGT TGTAAGTAGC AAAGGAGAAA CACAGGAATG TGTAAAATTA 
ACAGACATGA ATCAGAAACC AAAAACTGAA TGTTTTGCTG TGTGTGGCAC AGAAGAGACT 
CAGGCTTGTG TAAAACCACT GGGAAAGAAA AAAGGCAGGA TACTAGGTAA TAGTGTTGGT 
AAAAATAAAA TTAATGCTGG GTGTTATTCA GAATTAGATA AAATGCGTAC CGTATATGTT 
AGGGTACAGC GATTGTCATT GAATAAAATT CCCCAGAGTG CAGGAATCTC CACAGAACAT 
CTGAGGAGTG TGCTGCTTCT GAAGTCTGAG TATAGACAGG AATGTGGGGA AGCAAGAACA 
GAAGAGAGGA ATGATGCTAC AGATGTGACC TTCTTAAAAA AGAATCTCAG GAGTCCTAGA 
CAGCAGAAAT GCTGTGGATT CCCTTGTGTT TCAGAGCATA AGAGGAAAAA GGAGAGTGCA 
CAGCAGAAAA CTTTATGTTC TACACACAAC TGTTATTTCA CTGTTACAGA TCCAGCACAC 
ACGGTAAATG GAATAACGTG CATGCAAAAT CCATTGCTTG AAACAGCAAA CAATAGAAAA 
AGTAAATGTT CATATGGTGG TTCCCAGTCT TTTCTAAATC CTGTAGTGAA ATTGCATAAC 
TGCAGCAACA TGAAGAGGCT TATACGTTGC TCAAATCTGA GGGTAAGCAG TTCCAAAACT 
GAGAATACAT GTCCTGCAGC AACAGTAAAA GAACTTGATC CTCAAAAACA ATTACCTTGT 
TCTGTTAACA CTGTAAATTC AGTTATCAGT ACAGATTCTA CTGTTGGAGG TAATCATTCC 
AAAGAACTGG AATCTATTGC ACATGGCTTG GCAAATAGGA AAATTAAAAG AATATTAAAA 
GTAAAGTGGG ACTTTGGAAA AAAAATAAGG AATTCTGCAA CATGCTCTAC AGAGCGTACT 
CAAGAAGACT CTGCAATATC TGTCAAACAG AAAACAGGAG ACCTAAAATA TAGTGGTAAT 
CCTACTGTCA ACACAGACTC ATCAGATCCT TGCATTGGTA CTTTGGAAAA GATTATTGAC 
GAGATGAACA ATAATAAAAG TGACTCTGTA AATTTTAACT TGAAGGATGC CCTTTGTGAT 
CAAAGCACAA GTCAAAACAA AAAGGAAAGT TATGATTGCC TTCCGGTACA ATGCAGGACT 
ACACAATTAC CGTTTATGGT TTCAGAGTGT GAAAGAAAGG ATAGGGAATC CCTTGACATA 
GAGTGTTCTG CACTTATTAG AATGTCTGCA AATGAGTCAG TGGTCGATGA CTATTCAGAT 
TTTTCACTTA CTGAGGAAAA TGTTAACTTT GTAAATAATA ACAATATAAA TGAGCATACA 
GAAGAATGCA AAACCTGTTC CTGTCAGAGA ACAATCCCAT TTACTGGGAA AACCATCTGG 
AAATGTTCTT GTGCAAGGAC GTGTGTATGG GCTTTCCCAA GAAAAAGAAC TTCCTCTTTA 
CCAAGTACAG ATATACTGGG ATTTGACTCT GAATTGTCTT TTGCCCCTTA TTCCACCAAT 
ATAAAGTCTT TATTTCAGGC TAATGAAGCA GAGCCATTGC AGAGCACAAC TGATGACACT 
GACAGTGACG GGATATGTAA AAAACTAAAG TTAGATTTCA TTTCAGAAAA TGACGCTCTT 
ATTCTTAAAG ATATTGATGA TGACATTTTG TCTCACCCTC CAACAGAAAC TTTTGAACTA 
CCCAATACTG TAATTGAGAA GATATGTAAT ATAACTGAAA ATGTTTACTC ACTAGGTGAA 
GTTGAAGACA TGTATAAAAA TTTAGATGGT TGGCCATGCA GTTCAACTAT TATTCAAAAT 
ACTATAAATA GTCTTCCTCT CCCGCTGCCA AACAATGACA AAAGTGAGCA CTTTACCACT 
TCTGGCACTT ATTCTGAAAT AGAACCTACT GTAAAAACAG TTGAGGAGAT GGCCCTGAGC 
AGTTCTTTTG AAAACTCTGA TCCAAGTGTT TGTTCCAGAG ACAGCTCCAC AGAACCTGAA 
AACAGTTCAG AGACTGTGCT AAAAAGTACT CAATATGAAT CTCCAATACG TGATTGTTTA 
GACAAGCATA AAAAAAACAC TAATGTTGAG GAAGTGTTAT CCTTTTTTGA TTGTTTACAT 
GAAACTACAA TGGATAACGC CTGTCTAGCT GAGGAAAATT GCACCAGACA GATTAATAAT 
ACTGGTGGCC CATTAGATGA TTCACATTTG ACACTAAGCC ATGTAGAATC TCCAAAGAAA 
AGCAAGCTTG TGGAATACAA AAGTAATTTA GATGTGATAA AAGCTTATGA AGAAGATGTA 
CTGTTTTTAG ATGTTGTTCC AGATGATCCA GAGCTTTTTG GGACTCCTGA AGATCCTTAC 
ATAAGTCCAA TTTGTAATGC TGACAACAAA GAAAATAATA AACGGAATAA TCTGAGTGCA 
TTAATGTGTA ACCTTCAGGA ACAGGAGAGC GCCCATATCA ATGAAAGCAC AAATGATGCC 
AGCCCTGAGG AAAATGTAGA AGAGAGAAGT ACATATTTTG CAAAAGTAAT CACTGATCTA 
GAAGTTAATG ATGATCCTCT CATCATACAT AACACTTTGG AAAAGAACAG TGAAGATTTT 
TATGATGGTG GACAGTTTGA TTTTGAGGCG TTCAGAGATC TTGACGCAGA TAAAGAGATT 
AAATATGCTG AAAAGAATTC ATCTGAAAGC GGAAGCAGTT TTGAAGAGTC CTTGTCCAAG 
GATACTTCTC CGGGGTCCTC TCATTCCAAT AGCCGTCGTT GGAGAAGAGA TCCCTCACAT 
ACCTATAAGC CTATGCATTG GATGAATGAT TTCAGATACT CTGGAAAATG CATGGGATTG 
ACGGATGTGA TTACTTTGGA TCAGAAAGAA CAGAGGAATA TTGATAATCT CTTTTACGAT 
TTTACACCTA TACAGAAGAC CTTTTTGCCC ATTAGATACT GCAAATATTT TTTTAATACT 
TTCCGTGGAT GCATTAAACC AGATTGTATG TACCAGCACG TACCATTCCA AAGGGATGAA 
AAGGTGTGCA TGGAGGTGAT TCACAAACTT GTGAATGAAA ATCACACAAC ATTATTAAAA 
CGTGCTGTTT GGATATTTAC TGCCTACTAC AGAATGTATA TGCCTGGTGT TCATTATGAC 
TCAAATCTGC TGACCAAAAT GCTCCGTGCC TTGTATGTTC GTCAGATGTG GGGAGATGTA 
TTTCAGTTGT TGGAAACTGG TGCCAATGTC AAAATATTGC CCTCCTCTGA AATGCTTATC 
AGGTTATTTG AAAATATTGG CTCTACGGGA TTAACAGCAG CTGTACCTAG TTTAGTGGAT 
GTATTCTGCA AGCTTGTGGA GGCTGGAATG ATGATGAAGC CAGAGCAAAT CAACATGTTA 
ATAACAACAC TGAATAATCT CCAGGCAACA AAAAATTATA TCAGTGTCAT TTTAGACATA 
AAAACAAGAA TTGAAATGCA GCTTTCAGAA AAGAACTGGC TGTGTAACTT GGATATAGCA 
GTTGCAGAAG TTGGGCATTG TAAAGAAAAA AATGATTGGA AGAAACTAGG AACATTATAT 
CTTAATCTGC GTACAGGGTG TGAGAATGTG ACTGATCTTA AGAAGTTTTC TAATTGCATT 
GTGGGGGCGC TTCAGAAAGT GCCAAGGAAT GATAAATCTG AAATTCCATA TTGTGACTTT 
GCAGACACAG TGTACAAAGA CGCCCAACTA AGTGAAATTG ACAAAAACAT TTTGGGAAGG 
ATTGGAATAT CCATCATGTA TCACTATTAC AGAAATAAAC AATCGCAAAA GGGAAAGAGG 
GTGCTCAGAA AACTTCAGGA AATGCAGATT AATTTCACAG TCCTAAAAGG ACTCACAGGG 
GAAGAGAGTA AAGCTACGCG CTGTCAAGTT GTGAACACAG CTGTAGAGAT CTTCTTGAAC 
TGTGAATACT TAAATGGTGC ACTGGGGGTT CTACGAGAGT CAGAGTGGAT CATTAACACA 
CAAGTGTGGC CATGTGAAAG AATGGATGTG CTGAAACGAC ATAACCTGTT GTGTACAATT 
GCACAGAAAA CACTGAATAA AAACATGTTT GATGTGTGTC TTGAGGTCCT TCAGAATCTG 
CCAGGACTGC AGTGCTCATT AACGGATGTG GACGTCTCTC AGTACAGCTT GCTTTTCAAC 
AAACTTTTGT CATCCTGCAT AGAAAATAAT ACACTTGGTG TATCCTCAAC TGTGATAGAT 
TTTATGATTG CAAAAAAAAT ACCACTTGAC TTTTTCTTAC TGAGAGCACT AATCACATCC 
TTGGGACGAA GCTGTCTATG GCTCAGAGCA AGAGAACTTT ATAAATGCGC TGTTTTGTTG 
GGTTGCTACC CGCCCATGGA AGGAAATCTG TATCGTAAAG TGCTCTTCAT CCCCTCATTC 
CTTACTGAGA TAGAAATGCT GTTATCCATT GAAATTTTCA TGGTGTCAAA TGCCAGTAGT 
ATTCAGTCAC CAGGAGGCAG CCATCAGACT CTGCAAATCA TTCTCAAAAG ATGTGAAGAA 
GAGAGAGTTC CGAATAAAGA TTATCAAAGA GGTAAAGCTA GCTATCAAGA TGCAGTGGAC 
CGGCTGATTC AGGCCTCTAG GCTATCAACA CCAAGATTAT TCATAAAGCA CCTGACTGTC 
AATAATGCCA ATGAACAGGT TTATATCCTT GATTACGGCA GCTCTCTCAA ATGGTTGCAT 
GAAAACATGA AATGGGCTGG GAAAGTATGG CTTTTTCAGG GGAAAATATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

topaz1 ( XM_012964409.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012819863.1 Xenopus tropicalis testis and ovary specific PAZ domain containing 1 (topaz1), transcript variant X1, mRNA.

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CloneID	OXa33111
Clone ID Related Accession (Same CDS sequence)	XM_012964409.2 , NM_001374744.1
Accession Version	XM_012964409.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5691bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	testis- and ovary-specific PAZ domain-containing protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012964409.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTATGT TGCACTCTAG AAAAATTAAG CTAGTTCAAC CTAACCGGTC TGAATCCAGC 
TCATTTGCTA AGATCTGTGA TGAGGCTGAT ACTGAAAAAA GTAGTTCAGT TCTTATCAGT 
CCAGCTCTCT CTCTACAGTA TTCCTCAAAA GCCATTGATC AGGATAGGAG TGTAAAGAAT 
CTGTCAGGTA GAAACCCAAA GGCCATAAGT GGTGTGAATG AGCCTTGTGC AATCCAGCAA 
TGTGGAAATA TAGCGGTGTC TACAGAATCT GACTTTATTA GAATGAATGA CACAAGGAAG 
GCAAGACTAG CACTTTCAGA TCCAGTTCTT GCTTCTAATG ACTCTGGAGA AAGTATTAAT 
GTTTCAAACT TGCCGTCTTG TCAAGTTAAA AGAAGAACCA GAAGGAAGGC AACTGATCTG 
CGCGGTGCGG AGTCAATTGA AAAAGAAAAG TGTGGAAGTT TAGTAGTAAA TCAGGAAGCA 
GCAATTGTGA ATACTGATAC TACTGTTCCA CTAGATATTG AGAATGTTTA CAGTCCTACT 
CATATGCTAA ATCAACGGGG ATTGTTGAAA AAAATAGGAA AGAGGCCAAC AATTAAGAAG 
GAAGATGTGA GTAGTGTAGG AGATAGACAA AGATGTTTAA AACTAGCAGG AAAGCAAAGT 
GGAAAGAAAC TGCAATCTGA GCAAAGTGTT GTGTGTGGCA AGGGAAAGAC TCAGGAATGT 
GTACAAATAA TAGGGAAAAA AAGAGGAAGG AGGGCAAAAA CTGATCAGAG TAGTGAGTGT 
GGCATAAGTG AGATTCAGCA AAGAAAGAAA AGTAGAAAAA AGGTAAAAAC TGATAAGAGT 
GATGTGTGTT GTTCAGGAGA GACATTGAAA TATGTTAATA AAATTAAAAG AGGGAGGAAA 
CTAAAAACTG ATCATAAAGT TGTAAGTAGC AAAGGAGAAA CACAGGAATG TGTAAAATTA 
ACAGACATGA ATCAGAAACC AAAAACTGAA TGTTTTGCTG TGTGTGGCAC AGAAGAGACT 
CAGGCTTGTG TAAAACCACT GGGAAAGAAA AAAGGCAGGA TACTAGGTAA TAGTGTTGGT 
AAAAATAAAA TTAATGCTGG GTGTTATTCA GAATTAGATA AAATGCGTAC CGTATATGTT 
AGGGTACAGC GATTGTCATT GAATAAAATT CCCCAGAGTG CAGGAATCTC CACAGAACAT 
CTGAGGAGTG TGCTGCTTCT GAAGTCTGAG TATAGACAGG AATGTGGGGA AGCAAGAACA 
GAAGAGAGGA ATGATGCTAC AGATGTGACC TTCTTAAAAA AGAATCTCAG GAGTCCTAGA 
CAGCAGAAAT GCTGTGGATT CCCTTGTGTT TCAGAGCATA AGAGGAAAAA GGAGAGTGCA 
CAGCAGAAAA CTTTATGTTC TACACACAAC TGTTATTTCA CTGTTACAGA TCCAGCACAC 
ACGGTAAATG GAATAACGTG CATGCAAAAT CCATTGCTTG AAACAGCAAA CAATAGAAAA 
AGTAAATGTT CATATGGTGG TTCCCAGTCT TTTCTAAATC CTGTAGTGAA ATTGCATAAC 
TGCAGCAACA TGAAGAGGCT TATACGTTGC TCAAATCTGA GGGTAAGCAG TTCCAAAACT 
GAGAATACAT GTCCTGCAGC AACAGTAAAA GAACTTGATC CTCAAAAACA ATTACCTTGT 
TCTGTTAACA CTGTAAATTC AGTTATCAGT ACAGATTCTA CTGTTGGAGG TAATCATTCC 
AAAGAACTGG AATCTATTGC ACATGGCTTG GCAAATAGGA AAATTAAAAG AATATTAAAA 
GTAAAGTGGG ACTTTGGAAA AAAAATAAGG AATTCTGCAA CATGCTCTAC AGAGCGTACT 
CAAGAAGACT CTGCAATATC TGTCAAACAG AAAACAGGAG ACCTAAAATA TAGTGGTAAT 
CCTACTGTCA ACACAGACTC ATCAGATCCT TGCATTGGTA CTTTGGAAAA GATTATTGAC 
GAGATGAACA ATAATAAAAG TGACTCTGTA AATTTTAACT TGAAGGATGC CCTTTGTGAT 
CAAAGCACAA GTCAAAACAA AAAGGAAAGT TATGATTGCC TTCCGGTACA ATGCAGGACT 
ACACAATTAC CGTTTATGGT TTCAGAGTGT GAAAGAAAGG ATAGGGAATC CCTTGACATA 
GAGTGTTCTG CACTTATTAG AATGTCTGCA AATGAGTCAG TGGTCGATGA CTATTCAGAT 
TTTTCACTTA CTGAGGAAAA TGTTAACTTT GTAAATAATA ACAATATAAA TGAGCATACA 
GAAGAATGCA AAACCTGTTC CTGTCAGAGA ACAATCCCAT TTACTGGGAA AACCATCTGG 
AAATGTTCTT GTGCAAGGAC GTGTGTATGG GCTTTCCCAA GAAAAAGAAC TTCCTCTTTA 
CCAAGTACAG ATATACTGGG ATTTGACTCT GAATTGTCTT TTGCCCCTTA TTCCACCAAT 
ATAAAGTCTT TATTTCAGGC TAATGAAGCA GAGCCATTGC AGAGCACAAC TGATGACACT 
GACAGTGACG GGATATGTAA AAAACTAAAG TTAGATTTCA TTTCAGAAAA TGACGCTCTT 
ATTCTTAAAG ATATTGATGA TGACATTTTG TCTCACCCTC CAACAGAAAC TTTTGAACTA 
CCCAATACTG TAATTGAGAA GATATGTAAT ATAACTGAAA ATGTTTACTC ACTAGGTGAA 
GTTGAAGACA TGTATAAAAA TTTAGATGGT TGGCCATGCA GTTCAACTAT TATTCAAAAT 
ACTATAAATA GTCTTCCTCT CCCGCTGCCA AACAATGACA AAAGTGAGCA CTTTACCACT 
TCTGGCACTT ATTCTGAAAT AGAACCTACT GTAAAAACAG TTGAGGAGAT GGCCCTGAGC 
AGTTCTTTTG AAAACTCTGA TCCAAGTGTT TGTTCCAGAG ACAGCTCCAC AGAACCTGAA 
AACAGTTCAG AGACTGTGCT AAAAAGTACT CAATATGAAT CTCCAATACG TGATTGTTTA 
GACAAGCATA AAAAAAACAC TAATGTTGAG GAAGTGTTAT CCTTTTTTGA TTGTTTACAT 
GAAACTACAA TGGATAACGC CTGTCTAGCT GAGGAAAATT GCACCAGACA GATTAATAAT 
ACTGGTGGCC CATTAGATGA TTCACATTTG ACACTAAGCC ATGTAGAATC TCCAAAGAAA 
AGCAAGCTTG TGGAATACAA AAGTAATTTA GATGTGATAA AAGCTTATGA AGAAGATGTA 
CTGTTTTTAG ATGTTGTTCC AGATGATCCA GAGCTTTTTG GGACTCCTGA AGATCCTTAC 
ATAAGTCCAA TTTGTAATGC TGACAACAAA GAAAATAATA AACGGAATAA TCTGAGTGCA 
TTAATGTGTA ACCTTCAGGA ACAGGAGAGC GCCCATATCA ATGAAAGCAC AAATGATGCC 
AGCCCTGAGG AAAATGTAGA AGAGAGAAGT ACATATTTTG CAAAAGTAAT CACTGATCTA 
GAAGTTAATG ATGATCCTCT CATCATACAT AACACTTTGG AAAAGAACAG TGAAGATTTT 
TATGATGGTG GACAGTTTGA TTTTGAGGCG TTCAGAGATC TTGACGCAGA TAAAGAGATT 
AAATATGCTG AAAAGAATTC ATCTGAAAGC GGAAGCAGTT TTGAAGAGTC CTTGTCCAAG 
GATACTTCTC CGGGGTCCTC TCATTCCAAT AGCCGTCGTT GGAGAAGAGA TCCCTCACAT 
ACCTATAAGC CTATGCATTG GATGAATGAT TTCAGATACT CTGGAAAATG CATGGGATTG 
ACGGATGTGA TTACTTTGGA TCAGAAAGAA CAGAGGAATA TTGATAATCT CTTTTACGAT 
TTTACACCTA TACAGAAGAC CTTTTTGCCC ATTAGATACT GCAAATATTT TTTTAATACT 
TTCCGTGGAT GCATTAAACC AGATTGTATG TACCAGCACG TACCATTCCA AAGGGATGAA 
AAGGTGTGCA TGGAGGTGAT TCACAAACTT GTGAATGAAA ATCACACAAC ATTATTAAAA 
CGTGCTGTTT GGATATTTAC TGCCTACTAC AGAATGTATA TGCCTGGTGT TCATTATGAC 
TCAAATCTGC TGACCAAAAT GCTCCGTGCC TTGTATGTTC GTCAGATGTG GGGAGATGTA 
TTTCAGTTGT TGGAAACTGG TGCCAATGTC AAAATATTGC CCTCCTCTGA AATGCTTATC 
AGGTTATTTG AAAATATTGG CTCTACGGGA TTAACAGCAG CTGTACCTAG TTTAGTGGAT 
GTATTCTGCA AGCTTGTGGA GGCTGGAATG ATGATGAAGC CAGAGCAAAT CAACATGTTA 
ATAACAACAC TGAATAATCT CCAGGCAACA AAAAATTATA TCAGTGTCAT TTTAGACATA 
AAAACAAGAA TTGAAATGCA GCTTTCAGAA AAGAACTGGC TGTGTAACTT GGATATAGCA 
GTTGCAGAAG TTGGGCATTG TAAAGAAAAA AATGATTGGA AGAAACTAGG AACATTATAT 
CTTAATCTGC GTACAGGGTG TGAGAATGTG ACTGATCTTA AGAAGTTTTC TAATTGCATT 
GTGGGGGCGC TTCAGAAAGT GCCAAGGAAT GATAAATCTG AAATTCCATA TTGTGACTTT 
GCAGACACAG TGTACAAAGA CGCCCAACTA AGTGAAATTG ACAAAAACAT TTTGGGAAGG 
ATTGGAATAT CCATCATGTA TCACTATTAC AGAAATAAAC AATCGCAAAA GGGAAAGAGG 
GTGCTCAGAA AACTTCAGGA AATGCAGATT AATTTCACAG TCCTAAAAGG ACTCACAGGG 
GAAGAGAGTA AAGCTACGCG CTGTCAAGTT GTGAACACAG CTGTAGAGAT CTTCTTGAAC 
TGTGAATACT TAAATGGTGC ACTGGGGGTT CTACGAGAGT CAGAGTGGAT CATTAACACA 
CAAGTGTGGC CATGTGAAAG AATGGATGTG CTGAAACGAC ATAACCTGTT GTGTACAATT 
GCACAGAAAA CACTGAATAA AAACATGTTT GATGTGTGTC TTGAGGTCCT TCAGAATCTG 
CCAGGACTGC AGTGCTCATT AACGGATGTG GACGTCTCTC AGTACAGCTT GCTTTTCAAC 
AAACTTTTGT CATCCTGCAT AGAAAATAAT ACACTTGGTG TATCCTCAAC TGTGATAGAT 
TTTATGATTG CAAAAAAAAT ACCACTTGAC TTTTTCTTAC TGAGAGCACT AATCACATCC 
TTGGGACGAA GCTGTCTATG GCTCAGAGCA AGAGAACTTT ATAAATGCGC TGTTTTGTTG 
GGTTGCTACC CGCCCATGGA AGGAAATCTG TATCGTAAAG TGCTCTTCAT CCCCTCATTC 
CTTACTGAGA TAGAAATGCT GTTATCCATT GAAATTTTCA TGGTGTCAAA TGCCAGTAGT 
ATTCAGTCAC CAGGAGGCAG CCATCAGACT CTGCAAATCA TTCTCAAAAG ATGTGAAGAA 
GAGAGAGTTC CGAATAAAGA TTATCAAAGA GGTAAAGCTA GCTATCAAGA TGCAGTGGAC 
CGGCTGATTC AGGCCTCTAG GCTATCAACA CCAAGATTAT TCATAAAGCA CCTGACTGTC 
AATAATGCCA ATGAACAGGT TTATATCCTT GATTACGGCA GCTCTCTCAA ATGGTTGCAT 
GAAAACATGA AATGGGCTGG GAAAGTATGG CTTTTTCAGG GGAAAATATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012819863.1
CDS	777..6467
Translation	MSMLHSRKIKLVQPNRSESSSFAKICDEADTEKSSSVLISPALSLQYSSKAIDQDRSVKNLSGRNPKAISGVNEPCAIQQCGNIAVSTESDFIRMNDTRKARLALSDPVLASNDSGESINVSNLPSCQVKRRTRRKATDLRGAESIEKEKCGSLVVNQEAAIVNTDTTVPLDIENVYSPTHMLNQRGLLKKIGKRPTIKKEDVSSVGDRQRCLKLAGKQSGKKLQSEQSVVCGKGKTQECVQIIGKKRGRRAKTDQSSECGISEIQQRKKSRKKVKTDKSDVCCSGETLKYVNKIKRGRKLKTDHKVVSSKGETQECVKLTDMNQKPKTECFAVCGTEETQACVKPLGKKKGRILGNSVGKNKINAGCYSELDKMRTVYVRVQRLSLNKIPQSAGISTEHLRSVLLLKSEYRQECGEARTEERNDATDVTFLKKNLRSPRQQKCCGFPCVSEHKRKKESAQQKTLCSTHNCYFTVTDPAHTVNGITCMQNPLLETANNRKSKCSYGGSQSFLNPVVKLHNCSNMKRLIRCSNLRVSSSKTENTCPAATVKELDPQKQLPCSVNTVNSVISTDSTVGGNHSKELESIAHGLANRKIKRILKVKWDFGKKIRNSATCSTERTQEDSAISVKQKTGDLKYSGNPTVNTDSSDPCIGTLEKIIDEMNNNKSDSVNFNLKDALCDQSTSQNKKESYDCLPVQCRTTQLPFMVSECERKDRESLDIECSALIRMSANESVVDDYSDFSLTEENVNFVNNNNINEHTEECKTCSCQRTIPFTGKTIWKCSCARTCVWAFPRKRTSSLPSTDILGFDSELSFAPYSTNIKSLFQANEAEPLQSTTDDTDSDGICKKLKLDFISENDALILKDIDDDILSHPPTETFELPNTVIEKICNITENVYSLGEVEDMYKNLDGWPCSSTIIQNTINSLPLPLPNNDKSEHFTTSGTYSEIEPTVKTVEEMALSSSFENSDPSVCSRDSSTEPENSSETVLKSTQYESPIRDCLDKHKKNTNVEEVLSFFDCLHETTMDNACLAEENCTRQINNTGGPLDDSHLTLSHVESPKKSKLVEYKSNLDVIKAYEEDVLFLDVVPDDPELFGTPEDPYISPICNADNKENNKRNNLSALMCNLQEQESAHINESTNDASPEENVEERSTYFAKVITDLEVNDDPLIIHNTLEKNSEDFYDGGQFDFEAFRDLDADKEIKYAEKNSSESGSSFEESLSKDTSPGSSHSNSRRWRRDPSHTYKPMHWMNDFRYSGKCMGLTDVITLDQKEQRNIDNLFYDFTPIQKTFLPIRYCKYFFNTFRGCIKPDCMYQHVPFQRDEKVCMEVIHKLVNENHTTLLKRAVWIFTAYYRMYMPGVHYDSNLLTKMLRALYVRQMWGDVFQLLETGANVKILPSSEMLIRLFENIGSTGLTAAVPSLVDVFCKLVEAGMMMKPEQINMLITTLNNLQATKNYISVILDIKTRIEMQLSEKNWLCNLDIAVAEVGHCKEKNDWKKLGTLYLNLRTGCENVTDLKKFSNCIVGALQKVPRNDKSEIPYCDFADTVYKDAQLSEIDKNILGRIGISIMYHYYRNKQSQKGKRVLRKLQEMQINFTVLKGLTGEESKATRCQVVNTAVEIFLNCEYLNGALGVLRESEWIINTQVWPCERMDVLKRHNLLCTIAQKTLNKNMFDVCLEVLQNLPGLQCSLTDVDVSQYSLLFNKLLSSCIENNTLGVSSTVIDFMIAKKIPLDFFLLRALITSLGRSCLWLRARELYKCAVLLGCYPPMEGNLYRKVLFIPSFLTEIEMLLSIEIFMVSNASSIQSPGGSHQTLQIILKRCEEERVPNKDYQRGKASYQDAVDRLIQASRLSTPRLFIKHLTVNNANEQVYILDYGSSLKWLHENMKWAGKVWLFQGKI

Target ORF information:

RefSeq Version	XM_012964409.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis testis and ovary specific PAZ domain containing 1 (topaz1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012964409.2

ORF Insert Sequence:

ATGAGTATGT TGCACTCTAG AAAAATTAAG CTAGTTCAAC CTAACCGGTC TGAATCCAGC 
TCATTTGCTA AGATCTGTGA TGAGGCTGAT ACTGAAAAAA GTAGTTCAGT TCTTATCAGT 
CCAGCTCTCT CTCTACAGTA TTCCTCAAAA GCCATTGATC AGGATAGGAG TGTAAAGAAT 
CTGTCAGGTA GAAACCCAAA GGCCATAAGT GGTGTGAATG AGCCTTGTGC AATCCAGCAA 
TGTGGAAATA TAGCGGTGTC TACAGAATCT GACTTTATTA GAATGAATGA CACAAGGAAG 
GCAAGACTAG CACTTTCAGA TCCAGTTCTT GCTTCTAATG ACTCTGGAGA AAGTATTAAT 
GTTTCAAACT TGCCGTCTTG TCAAGTTAAA AGAAGAACCA GAAGGAAGGC AACTGATCTG 
CGCGGTGCGG AGTCAATTGA AAAAGAAAAG TGTGGAAGTT TAGTAGTAAA TCAGGAAGCA 
GCAATTGTGA ATACTGATAC TACTGTTCCA CTAGATATTG AGAATGTTTA CAGTCCTACT 
CATATGCTAA ATCAACGGGG ATTGTTGAAA AAAATAGGAA AGAGGCCAAC AATTAAGAAG 
GAAGATGTGA GTAGTGTAGG AGATAGACAA AGATGTTTAA AACTAGCAGG AAAGCAAAGT 
GGAAAGAAAC TGCAATCTGA GCAAAGTGTT GTGTGTGGCA AGGGAAAGAC TCAGGAATGT 
GTACAAATAA TAGGGAAAAA AAGAGGAAGG AGGGCAAAAA CTGATCAGAG TAGTGAGTGT 
GGCATAAGTG AGATTCAGCA AAGAAAGAAA AGTAGAAAAA AGGTAAAAAC TGATAAGAGT 
GATGTGTGTT GTTCAGGAGA GACATTGAAA TATGTTAATA AAATTAAAAG AGGGAGGAAA 
CTAAAAACTG ATCATAAAGT TGTAAGTAGC AAAGGAGAAA CACAGGAATG TGTAAAATTA 
ACAGACATGA ATCAGAAACC AAAAACTGAA TGTTTTGCTG TGTGTGGCAC AGAAGAGACT 
CAGGCTTGTG TAAAACCACT GGGAAAGAAA AAAGGCAGGA TACTAGGTAA TAGTGTTGGT 
AAAAATAAAA TTAATGCTGG GTGTTATTCA GAATTAGATA AAATGCGTAC CGTATATGTT 
AGGGTACAGC GATTGTCATT GAATAAAATT CCCCAGAGTG CAGGAATCTC CACAGAACAT 
CTGAGGAGTG TGCTGCTTCT GAAGTCTGAG TATAGACAGG AATGTGGGGA AGCAAGAACA 
GAAGAGAGGA ATGATGCTAC AGATGTGACC TTCTTAAAAA AGAATCTCAG GAGTCCTAGA 
CAGCAGAAAT GCTGTGGATT CCCTTGTGTT TCAGAGCATA AGAGGAAAAA GGAGAGTGCA 
CAGCAGAAAA CTTTATGTTC TACACACAAC TGTTATTTCA CTGTTACAGA TCCAGCACAC 
ACGGTAAATG GAATAACGTG CATGCAAAAT CCATTGCTTG AAACAGCAAA CAATAGAAAA 
AGTAAATGTT CATATGGTGG TTCCCAGTCT TTTCTAAATC CTGTAGTGAA ATTGCATAAC 
TGCAGCAACA TGAAGAGGCT TATACGTTGC TCAAATCTGA GGGTAAGCAG TTCCAAAACT 
GAGAATACAT GTCCTGCAGC AACAGTAAAA GAACTTGATC CTCAAAAACA ATTACCTTGT 
TCTGTTAACA CTGTAAATTC AGTTATCAGT ACAGATTCTA CTGTTGGAGG TAATCATTCC 
AAAGAACTGG AATCTATTGC ACATGGCTTG GCAAATAGGA AAATTAAAAG AATATTAAAA 
GTAAAGTGGG ACTTTGGAAA AAAAATAAGG AATTCTGCAA CATGCTCTAC AGAGCGTACT 
CAAGAAGACT CTGCAATATC TGTCAAACAG AAAACAGGAG ACCTAAAATA TAGTGGTAAT 
CCTACTGTCA ACACAGACTC ATCAGATCCT TGCATTGGTA CTTTGGAAAA GATTATTGAC 
GAGATGAACA ATAATAAAAG TGACTCTGTA AATTTTAACT TGAAGGATGC CCTTTGTGAT 
CAAAGCACAA GTCAAAACAA AAAGGAAAGT TATGATTGCC TTCCGGTACA ATGCAGGACT 
ACACAATTAC CGTTTATGGT TTCAGAGTGT GAAAGAAAGG ATAGGGAATC CCTTGACATA 
GAGTGTTCTG CACTTATTAG AATGTCTGCA AATGAGTCAG TGGTCGATGA CTATTCAGAT 
TTTTCACTTA CTGAGGAAAA TGTTAACTTT GTAAATAATA ACAATATAAA TGAGCATACA 
GAAGAATGCA AAACCTGTTC CTGTCAGAGA ACAATCCCAT TTACTGGGAA AACCATCTGG 
AAATGTTCTT GTGCAAGGAC GTGTGTATGG GCTTTCCCAA GAAAAAGAAC TTCCTCTTTA 
CCAAGTACAG ATATACTGGG ATTTGACTCT GAATTGTCTT TTGCCCCTTA TTCCACCAAT 
ATAAAGTCTT TATTTCAGGC TAATGAAGCA GAGCCATTGC AGAGCACAAC TGATGACACT 
GACAGTGACG GGATATGTAA AAAACTAAAG TTAGATTTCA TTTCAGAAAA TGACGCTCTT 
ATTCTTAAAG ATATTGATGA TGACATTTTG TCTCACCCTC CAACAGAAAC TTTTGAACTA 
CCCAATACTG TAATTGAGAA GATATGTAAT ATAACTGAAA ATGTTTACTC ACTAGGTGAA 
GTTGAAGACA TGTATAAAAA TTTAGATGGT TGGCCATGCA GTTCAACTAT TATTCAAAAT 
ACTATAAATA GTCTTCCTCT CCCGCTGCCA AACAATGACA AAAGTGAGCA CTTTACCACT 
TCTGGCACTT ATTCTGAAAT AGAACCTACT GTAAAAACAG TTGAGGAGAT GGCCCTGAGC 
AGTTCTTTTG AAAACTCTGA TCCAAGTGTT TGTTCCAGAG ACAGCTCCAC AGAACCTGAA 
AACAGTTCAG AGACTGTGCT AAAAAGTACT CAATATGAAT CTCCAATACG TGATTGTTTA 
GACAAGCATA AAAAAAACAC TAATGTTGAG GAAGTGTTAT CCTTTTTTGA TTGTTTACAT 
GAAACTACAA TGGATAACGC CTGTCTAGCT GAGGAAAATT GCACCAGACA GATTAATAAT 
ACTGGTGGCC CATTAGATGA TTCACATTTG ACACTAAGCC ATGTAGAATC TCCAAAGAAA 
AGCAAGCTTG TGGAATACAA AAGTAATTTA GATGTGATAA AAGCTTATGA AGAAGATGTA 
CTGTTTTTAG ATGTTGTTCC AGATGATCCA GAGCTTTTTG GGACTCCTGA AGATCCTTAC 
ATAAGTCCAA TTTGTAATGC TGACAACAAA GAAAATAATA AACGGAATAA TCTGAGTGCA 
TTAATGTGTA ACCTTCAGGA ACAGGAGAGC GCCCATATCA ATGAAAGCAC AAATGATGCC 
AGCCCTGAGG AAAATGTAGA AGAGAGAAGT ACATATTTTG CAAAAGTAAT CACTGATCTA 
GAAGTTAATG ATGATCCTCT CATCATACAT AACACTTTGG AAAAGAACAG TGAAGATTTT 
TATGATGGTG GACAGTTTGA TTTTGAGGCG TTCAGAGATC TTGACGCAGA TAAAGAGATT 
AAATATGCTG AAAAGAATTC ATCTGAAAGC GGAAGCAGTT TTGAAGAGTC CTTGTCCAAG 
GATACTTCTC CGGGGTCCTC TCATTCCAAT AGCCGTCGTT GGAGAAGAGA TCCCTCACAT 
ACCTATAAGC CTATGCATTG GATGAATGAT TTCAGATACT CTGGAAAATG CATGGGATTG 
ACGGATGTGA TTACTTTGGA TCAGAAAGAA CAGAGGAATA TTGATAATCT CTTTTACGAT 
TTTACACCTA TACAGAAGAC CTTTTTGCCC ATTAGATACT GCAAATATTT TTTTAATACT 
TTCCGTGGAT GCATTAAACC AGATTGTATG TACCAGCACG TACCATTCCA AAGGGATGAA 
AAGGTGTGCA TGGAGGTGAT TCACAAACTT GTGAATGAAA ATCACACAAC ATTATTAAAA 
CGTGCTGTTT GGATATTTAC TGCCTACTAC AGAATGTATA TGCCTGGTGT TCATTATGAC 
TCAAATCTGC TGACCAAAAT GCTCCGTGCC TTGTATGTTC GTCAGATGTG GGGAGATGTA 
TTTCAGTTGT TGGAAACTGG TGCCAATGTC AAAATATTGC CCTCCTCTGA AATGCTTATC 
AGGTTATTTG AAAATATTGG CTCTACGGGA TTAACAGCAG CTGTACCTAG TTTAGTGGAT 
GTATTCTGCA AGCTTGTGGA GGCTGGAATG ATGATGAAGC CAGAGCAAAT CAACATGTTA 
ATAACAACAC TGAATAATCT CCAGGCAACA AAAAATTATA TCAGTGTCAT TTTAGACATA 
AAAACAAGAA TTGAAATGCA GCTTTCAGAA AAGAACTGGC TGTGTAACTT GGATATAGCA 
GTTGCAGAAG TTGGGCATTG TAAAGAAAAA AATGATTGGA AGAAACTAGG AACATTATAT 
CTTAATCTGC GTACAGGGTG TGAGAATGTG ACTGATCTTA AGAAGTTTTC TAATTGCATT 
GTGGGGGCGC TTCAGAAAGT GCCAAGGAAT GATAAATCTG AAATTCCATA TTGTGACTTT 
GCAGACACAG TGTACAAAGA CGCCCAACTA AGTGAAATTG ACAAAAACAT TTTGGGAAGG 
ATTGGAATAT CCATCATGTA TCACTATTAC AGAAATAAAC AATCGCAAAA GGGAAAGAGG 
GTGCTCAGAA AACTTCAGGA AATGCAGATT AATTTCACAG TCCTAAAAGG ACTCACAGGG 
GAAGAGAGTA AAGCTACGCG CTGTCAAGTT GTGAACACAG CTGTAGAGAT CTTCTTGAAC 
TGTGAATACT TAAATGGTGC ACTGGGGGTT CTACGAGAGT CAGAGTGGAT CATTAACACA 
CAAGTGTGGC CATGTGAAAG AATGGATGTG CTGAAACGAC ATAACCTGTT GTGTACAATT 
GCACAGAAAA CACTGAATAA AAACATGTTT GATGTGTGTC TTGAGGTCCT TCAGAATCTG 
CCAGGACTGC AGTGCTCATT AACGGATGTG GACGTCTCTC AGTACAGCTT GCTTTTCAAC 
AAACTTTTGT CATCCTGCAT AGAAAATAAT ACACTTGGTG TATCCTCAAC TGTGATAGAT 
TTTATGATTG CAAAAAAAAT ACCACTTGAC TTTTTCTTAC TGAGAGCACT AATCACATCC 
TTGGGACGAA GCTGTCTATG GCTCAGAGCA AGAGAACTTT ATAAATGCGC TGTTTTGTTG 
GGTTGCTACC CGCCCATGGA AGGAAATCTG TATCGTAAAG TGCTCTTCAT CCCCTCATTC 
CTTACTGAGA TAGAAATGCT GTTATCCATT GAAATTTTCA TGGTGTCAAA TGCCAGTAGT 
ATTCAGTCAC CAGGAGGCAG CCATCAGACT CTGCAAATCA TTCTCAAAAG ATGTGAAGAA 
GAGAGAGTTC CGAATAAAGA TTATCAAAGA GGTAAAGCTA GCTATCAAGA TGCAGTGGAC 
CGGCTGATTC AGGCCTCTAG GCTATCAACA CCAAGATTAT TCATAAAGCA CCTGACTGTC 
AATAATGCCA ATGAACAGGT TTATATCCTT GATTACGGCA GCTCTCTCAA ATGGTTGCAT 
GAAAACATGA AATGGGCTGG GAAAGTATGG CTTTTTCAGG GGAAAATATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

topaz1 ( XM_012964412.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012819866.1 Xenopus tropicalis testis and ovary specific PAZ domain containing 1 (topaz1), transcript variant X2, mRNA.

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CloneID	OXa33112
Clone ID Related Accession (Same CDS sequence)	XM_012964412.2 , XM_012964413.2 , XM_012964411.2
Accession Version	XM_012964412.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4230bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	testis- and ovary-specific PAZ domain-containing protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012964412.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAAATC CATTGCTTGA AACAGCAAAC AATAGAAAAA GTAAATGTTC ATATGGTGGT 
TCCCAGTCTT TTCTAAATCC TGTAGTGAAA TTGCATAACT GCAGCAACAT GAAGAGGCTT 
ATACGTTGCT CAAATCTGAG GGTAAGCAGT TCCAAAACTG AGAATACATG TCCTGCAGCA 
ACAGTAAAAG AACTTGATCC TCAAAAACAA TTACCTTGTT CTGTTAACAC TGTAAATTCA 
GTTATCAGTA CAGATTCTAC TGTTGGAGGT AATCATTCCA AAGAACTGGA ATCTATTGCA 
CATGGCTTGG CAAATAGGAA AATTAAAAGA ATATTAAAAG TAAAGTGGGA CTTTGGAAAA 
AAAATAAGGA ATTCTGCAAC ATGCTCTACA GAGCGTACTC AAGAAGACTC TGCAATATCT 
GTCAAACAGA AAACAGGAGA CCTAAAATAT AGTGGTAATC CTACTGTCAA CACAGACTCA 
TCAGATCCTT GCATTGGTAC TTTGGAAAAG ATTATTGACG AGATGAACAA TAATAAAAGT 
GACTCTGTAA ATTTTAACTT GAAGGATGCC CTTTGTGATC AAAGCACAAG TCAAAACAAA 
AAGGAAAGTT ATGATTGCCT TCCGGTACAA TGCAGGACTA CACAATTACC GTTTATGGTT 
TCAGAGTGTG AAAGAAAGGA TAGGGAATCC CTTGACATAG AGTGTTCTGC ACTTATTAGA 
ATGTCTGCAA ATGAGTCAGT GGTCGATGAC TATTCAGATT TTTCACTTAC TGAGGAAAAT 
GTTAACTTTG TAAATAATAA CAATATAAAT GAGCATACAG AAGAATGCAA AACCTGTTCC 
TGTCAGAGAA CAATCCCATT TACTGGGAAA ACCATCTGGA AATGTTCTTG TGCAAGGACG 
TGTGTATGGG CTTTCCCAAG AAAAAGAACT TCCTCTTTAC CAAGTACAGA TATACTGGGA 
TTTGACTCTG AATTGTCTTT TGCCCCTTAT TCCACCAATA TAAAGTCTTT ATTTCAGGCT 
AATGAAGCAG AGCCATTGCA GAGCACAACT GATGACACTG ACAGTGACGG GATATGTAAA 
AAACTAAAGT TAGATTTCAT TTCAGAAAAT GACGCTCTTA TTCTTAAAGA TATTGATGAT 
GACATTTTGT CTCACCCTCC AACAGAAACT TTTGAACTAC CCAATACTGT AATTGAGAAG 
ATATGTAATA TAACTGAAAA TGTTTACTCA CTAGGTGAAG TTGAAGACAT GTATAAAAAT 
TTAGATGGTT GGCCATGCAG TTCAACTATT ATTCAAAATA CTATAAATAG TCTTCCTCTC 
CCGCTGCCAA ACAATGACAA AAGTGAGCAC TTTACCACTT CTGGCACTTA TTCTGAAATA 
GAACCTACTG TAAAAACAGT TGAGGAGATG GCCCTGAGCA GTTCTTTTGA AAACTCTGAT 
CCAAGTGTTT GTTCCAGAGA CAGCTCCACA GAACCTGAAA ACAGTTCAGA GACTGTGCTA 
AAAAGTACTC AATATGAATC TCCAATACGT GATTGTTTAG ACAAGCATAA AAAAAACACT 
AATGTTGAGG AAGTGTTATC CTTTTTTGAT TGTTTACATG AAACTACAAT GGATAACGCC 
TGTCTAGCTG AGGAAAATTG CACCAGACAG ATTAATAATA CTGGTGGCCC ATTAGATGAT 
TCACATTTGA CACTAAGCCA TGTAGAATCT CCAAAGAAAA GCAAGCTTGT GGAATACAAA 
AGTAATTTAG ATGTGATAAA AGCTTATGAA GAAGATGTAC TGTTTTTAGA TGTTGTTCCA 
GATGATCCAG AGCTTTTTGG GACTCCTGAA GATCCTTACA TAAGTCCAAT TTGTAATGCT 
GACAACAAAG AAAATAATAA ACGGAATAAT CTGAGTGCAT TAATGTGTAA CCTTCAGGAA 
CAGGAGAGCG CCCATATCAA TGAAAGCACA AATGATGCCA GCCCTGAGGA AAATGTAGAA 
GAGAGAAGTA CATATTTTGC AAAAGTAATC ACTGATCTAG AAGTTAATGA TGATCCTCTC 
ATCATACATA ACACTTTGGA AAAGAACAGT GAAGATTTTT ATGATGGTGG ACAGTTTGAT 
TTTGAGGCGT TCAGAGATCT TGACGCAGAT AAAGAGATTA AATATGCTGA AAAGAATTCA 
TCTGAAAGCG GAAGCAGTTT TGAAGAGTCC TTGTCCAAGG ATACTTCTCC GGGGTCCTCT 
CATTCCAATA GCCGTCGTTG GAGAAGAGAT CCCTCACATA CCTATAAGCC TATGCATTGG 
ATGAATGATT TCAGATACTC TGGAAAATGC ATGGGATTGA CGGATGTGAT TACTTTGGAT 
CAGAAAGAAC AGAGGAATAT TGATAATCTC TTTTACGATT TTACACCTAT ACAGAAGACC 
TTTTTGCCCA TTAGATACTG CAAATATTTT TTTAATACTT TCCGTGGATG CATTAAACCA 
GATTGTATGT ACCAGCACGT ACCATTCCAA AGGGATGAAA AGGTGTGCAT GGAGGTGATT 
CACAAACTTG TGAATGAAAA TCACACAACA TTATTAAAAC GTGCTGTTTG GATATTTACT 
GCCTACTACA GAATGTATAT GCCTGGTGTT CATTATGACT CAAATCTGCT GACCAAAATG 
CTCCGTGCCT TGTATGTTCG TCAGATGTGG GGAGATGTAT TTCAGTTGTT GGAAACTGGT 
GCCAATGTCA AAATATTGCC CTCCTCTGAA ATGCTTATCA GGTTATTTGA AAATATTGGC 
TCTACGGGAT TAACAGCAGC TGTACCTAGT TTAGTGGATG TATTCTGCAA GCTTGTGGAG 
GCTGGAATGA TGATGAAGCC AGAGCAAATC AACATGTTAA TAACAACACT GAATAATCTC 
CAGGCAACAA AAAATTATAT CAGTGTCATT TTAGACATAA AAACAAGAAT TGAAATGCAG 
CTTTCAGAAA AGAACTGGCT GTGTAACTTG GATATAGCAG TTGCAGAAGT TGGGCATTGT 
AAAGAAAAAA ATGATTGGAA GAAACTAGGA ACATTATATC TTAATCTGCG TACAGGGTGT 
GAGAATGTGA CTGATCTTAA GAAGTTTTCT AATTGCATTG TGGGGGCGCT TCAGAAAGTG 
CCAAGGAATG ATAAATCTGA AATTCCATAT TGTGACTTTG CAGACACAGT GTACAAAGAC 
GCCCAACTAA GTGAAATTGA CAAAAACATT TTGGGAAGGA TTGGAATATC CATCATGTAT 
CACTATTACA GAAATAAACA ATCGCAAAAG GGAAAGAGGG TGCTCAGAAA ACTTCAGGAA 
ATGCAGATTA ATTTCACAGT CCTAAAAGGA CTCACAGGGG AAGAGAGTAA AGCTACGCGC 
TGTCAAGTTG TGAACACAGC TGTAGAGATC TTCTTGAACT GTGAATACTT AAATGGTGCA 
CTGGGGGTTC TACGAGAGTC AGAGTGGATC ATTAACACAC AAGTGTGGCC ATGTGAAAGA 
ATGGATGTGC TGAAACGACA TAACCTGTTG TGTACAATTG CACAGAAAAC ACTGAATAAA 
AACATGTTTG ATGTGTGTCT TGAGGTCCTT CAGAATCTGC CAGGACTGCA GTGCTCATTA 
ACGGATGTGG ACGTCTCTCA GTACAGCTTG CTTTTCAACA AACTTTTGTC ATCCTGCATA 
GAAAATAATA CACTTGGTGT ATCCTCAACT GTGATAGATT TTATGATTGC AAAAAAAATA 
CCACTTGACT TTTTCTTACT GAGAGCACTA ATCACATCCT TGGGACGAAG CTGTCTATGG 
CTCAGAGCAA GAGAACTTTA TAAATGCGCT GTTTTGTTGG GTTGCTACCC GCCCATGGAA 
GGAAATCTGT ATCGTAAAGT GCTCTTCATC CCCTCATTCC TTACTGAGAT AGAAATGCTG 
TTATCCATTG AAATTTTCAT GGTGTCAAAT GCCAGTAGTA TTCAGTCACC AGGAGGCAGC 
CATCAGACTC TGCAAATCAT TCTCAAAAGA TGTGAAGAAG AGAGAGTTCC GAATAAAGAT 
TATCAAAGAG GTAAAGCTAG CTATCAAGAT GCAGTGGACC GGCTGATTCA GGCCTCTAGG 
CTATCAACAC CAAGATTATT CATAAAGCAC CTGACTGTCA ATAATGCCAA TGAACAGGTT 
TATATCCTTG ATTACGGCAG CTCTCTCAAA TGGTTGCATG AAAACATGAA ATGGGCTGGG 
AAAGTATGGC TTTTTCAGGG GAAAATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012819866.1
CDS	290..4519
Translation	MQNPLLETANNRKSKCSYGGSQSFLNPVVKLHNCSNMKRLIRCSNLRVSSSKTENTCPAATVKELDPQKQLPCSVNTVNSVISTDSTVGGNHSKELESIAHGLANRKIKRILKVKWDFGKKIRNSATCSTERTQEDSAISVKQKTGDLKYSGNPTVNTDSSDPCIGTLEKIIDEMNNNKSDSVNFNLKDALCDQSTSQNKKESYDCLPVQCRTTQLPFMVSECERKDRESLDIECSALIRMSANESVVDDYSDFSLTEENVNFVNNNNINEHTEECKTCSCQRTIPFTGKTIWKCSCARTCVWAFPRKRTSSLPSTDILGFDSELSFAPYSTNIKSLFQANEAEPLQSTTDDTDSDGICKKLKLDFISENDALILKDIDDDILSHPPTETFELPNTVIEKICNITENVYSLGEVEDMYKNLDGWPCSSTIIQNTINSLPLPLPNNDKSEHFTTSGTYSEIEPTVKTVEEMALSSSFENSDPSVCSRDSSTEPENSSETVLKSTQYESPIRDCLDKHKKNTNVEEVLSFFDCLHETTMDNACLAEENCTRQINNTGGPLDDSHLTLSHVESPKKSKLVEYKSNLDVIKAYEEDVLFLDVVPDDPELFGTPEDPYISPICNADNKENNKRNNLSALMCNLQEQESAHINESTNDASPEENVEERSTYFAKVITDLEVNDDPLIIHNTLEKNSEDFYDGGQFDFEAFRDLDADKEIKYAEKNSSESGSSFEESLSKDTSPGSSHSNSRRWRRDPSHTYKPMHWMNDFRYSGKCMGLTDVITLDQKEQRNIDNLFYDFTPIQKTFLPIRYCKYFFNTFRGCIKPDCMYQHVPFQRDEKVCMEVIHKLVNENHTTLLKRAVWIFTAYYRMYMPGVHYDSNLLTKMLRALYVRQMWGDVFQLLETGANVKILPSSEMLIRLFENIGSTGLTAAVPSLVDVFCKLVEAGMMMKPEQINMLITTLNNLQATKNYISVILDIKTRIEMQLSEKNWLCNLDIAVAEVGHCKEKNDWKKLGTLYLNLRTGCENVTDLKKFSNCIVGALQKVPRNDKSEIPYCDFADTVYKDAQLSEIDKNILGRIGISIMYHYYRNKQSQKGKRVLRKLQEMQINFTVLKGLTGEESKATRCQVVNTAVEIFLNCEYLNGALGVLRESEWIINTQVWPCERMDVLKRHNLLCTIAQKTLNKNMFDVCLEVLQNLPGLQCSLTDVDVSQYSLLFNKLLSSCIENNTLGVSSTVIDFMIAKKIPLDFFLLRALITSLGRSCLWLRARELYKCAVLLGCYPPMEGNLYRKVLFIPSFLTEIEMLLSIEIFMVSNASSIQSPGGSHQTLQIILKRCEEERVPNKDYQRGKASYQDAVDRLIQASRLSTPRLFIKHLTVNNANEQVYILDYGSSLKWLHENMKWAGKVWLFQGKI

Target ORF information:

RefSeq Version	XM_012964412.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis testis and ovary specific PAZ domain containing 1 (topaz1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012964412.2

ORF Insert Sequence:

ATGCAAAATC CATTGCTTGA AACAGCAAAC AATAGAAAAA GTAAATGTTC ATATGGTGGT 
TCCCAGTCTT TTCTAAATCC TGTAGTGAAA TTGCATAACT GCAGCAACAT GAAGAGGCTT 
ATACGTTGCT CAAATCTGAG GGTAAGCAGT TCCAAAACTG AGAATACATG TCCTGCAGCA 
ACAGTAAAAG AACTTGATCC TCAAAAACAA TTACCTTGTT CTGTTAACAC TGTAAATTCA 
GTTATCAGTA CAGATTCTAC TGTTGGAGGT AATCATTCCA AAGAACTGGA ATCTATTGCA 
CATGGCTTGG CAAATAGGAA AATTAAAAGA ATATTAAAAG TAAAGTGGGA CTTTGGAAAA 
AAAATAAGGA ATTCTGCAAC ATGCTCTACA GAGCGTACTC AAGAAGACTC TGCAATATCT 
GTCAAACAGA AAACAGGAGA CCTAAAATAT AGTGGTAATC CTACTGTCAA CACAGACTCA 
TCAGATCCTT GCATTGGTAC TTTGGAAAAG ATTATTGACG AGATGAACAA TAATAAAAGT 
GACTCTGTAA ATTTTAACTT GAAGGATGCC CTTTGTGATC AAAGCACAAG TCAAAACAAA 
AAGGAAAGTT ATGATTGCCT TCCGGTACAA TGCAGGACTA CACAATTACC GTTTATGGTT 
TCAGAGTGTG AAAGAAAGGA TAGGGAATCC CTTGACATAG AGTGTTCTGC ACTTATTAGA 
ATGTCTGCAA ATGAGTCAGT GGTCGATGAC TATTCAGATT TTTCACTTAC TGAGGAAAAT 
GTTAACTTTG TAAATAATAA CAATATAAAT GAGCATACAG AAGAATGCAA AACCTGTTCC 
TGTCAGAGAA CAATCCCATT TACTGGGAAA ACCATCTGGA AATGTTCTTG TGCAAGGACG 
TGTGTATGGG CTTTCCCAAG AAAAAGAACT TCCTCTTTAC CAAGTACAGA TATACTGGGA 
TTTGACTCTG AATTGTCTTT TGCCCCTTAT TCCACCAATA TAAAGTCTTT ATTTCAGGCT 
AATGAAGCAG AGCCATTGCA GAGCACAACT GATGACACTG ACAGTGACGG GATATGTAAA 
AAACTAAAGT TAGATTTCAT TTCAGAAAAT GACGCTCTTA TTCTTAAAGA TATTGATGAT 
GACATTTTGT CTCACCCTCC AACAGAAACT TTTGAACTAC CCAATACTGT AATTGAGAAG 
ATATGTAATA TAACTGAAAA TGTTTACTCA CTAGGTGAAG TTGAAGACAT GTATAAAAAT 
TTAGATGGTT GGCCATGCAG TTCAACTATT ATTCAAAATA CTATAAATAG TCTTCCTCTC 
CCGCTGCCAA ACAATGACAA AAGTGAGCAC TTTACCACTT CTGGCACTTA TTCTGAAATA 
GAACCTACTG TAAAAACAGT TGAGGAGATG GCCCTGAGCA GTTCTTTTGA AAACTCTGAT 
CCAAGTGTTT GTTCCAGAGA CAGCTCCACA GAACCTGAAA ACAGTTCAGA GACTGTGCTA 
AAAAGTACTC AATATGAATC TCCAATACGT GATTGTTTAG ACAAGCATAA AAAAAACACT 
AATGTTGAGG AAGTGTTATC CTTTTTTGAT TGTTTACATG AAACTACAAT GGATAACGCC 
TGTCTAGCTG AGGAAAATTG CACCAGACAG ATTAATAATA CTGGTGGCCC ATTAGATGAT 
TCACATTTGA CACTAAGCCA TGTAGAATCT CCAAAGAAAA GCAAGCTTGT GGAATACAAA 
AGTAATTTAG ATGTGATAAA AGCTTATGAA GAAGATGTAC TGTTTTTAGA TGTTGTTCCA 
GATGATCCAG AGCTTTTTGG GACTCCTGAA GATCCTTACA TAAGTCCAAT TTGTAATGCT 
GACAACAAAG AAAATAATAA ACGGAATAAT CTGAGTGCAT TAATGTGTAA CCTTCAGGAA 
CAGGAGAGCG CCCATATCAA TGAAAGCACA AATGATGCCA GCCCTGAGGA AAATGTAGAA 
GAGAGAAGTA CATATTTTGC AAAAGTAATC ACTGATCTAG AAGTTAATGA TGATCCTCTC 
ATCATACATA ACACTTTGGA AAAGAACAGT GAAGATTTTT ATGATGGTGG ACAGTTTGAT 
TTTGAGGCGT TCAGAGATCT TGACGCAGAT AAAGAGATTA AATATGCTGA AAAGAATTCA 
TCTGAAAGCG GAAGCAGTTT TGAAGAGTCC TTGTCCAAGG ATACTTCTCC GGGGTCCTCT 
CATTCCAATA GCCGTCGTTG GAGAAGAGAT CCCTCACATA CCTATAAGCC TATGCATTGG 
ATGAATGATT TCAGATACTC TGGAAAATGC ATGGGATTGA CGGATGTGAT TACTTTGGAT 
CAGAAAGAAC AGAGGAATAT TGATAATCTC TTTTACGATT TTACACCTAT ACAGAAGACC 
TTTTTGCCCA TTAGATACTG CAAATATTTT TTTAATACTT TCCGTGGATG CATTAAACCA 
GATTGTATGT ACCAGCACGT ACCATTCCAA AGGGATGAAA AGGTGTGCAT GGAGGTGATT 
CACAAACTTG TGAATGAAAA TCACACAACA TTATTAAAAC GTGCTGTTTG GATATTTACT 
GCCTACTACA GAATGTATAT GCCTGGTGTT CATTATGACT CAAATCTGCT GACCAAAATG 
CTCCGTGCCT TGTATGTTCG TCAGATGTGG GGAGATGTAT TTCAGTTGTT GGAAACTGGT 
GCCAATGTCA AAATATTGCC CTCCTCTGAA ATGCTTATCA GGTTATTTGA AAATATTGGC 
TCTACGGGAT TAACAGCAGC TGTACCTAGT TTAGTGGATG TATTCTGCAA GCTTGTGGAG 
GCTGGAATGA TGATGAAGCC AGAGCAAATC AACATGTTAA TAACAACACT GAATAATCTC 
CAGGCAACAA AAAATTATAT CAGTGTCATT TTAGACATAA AAACAAGAAT TGAAATGCAG 
CTTTCAGAAA AGAACTGGCT GTGTAACTTG GATATAGCAG TTGCAGAAGT TGGGCATTGT 
AAAGAAAAAA ATGATTGGAA GAAACTAGGA ACATTATATC TTAATCTGCG TACAGGGTGT 
GAGAATGTGA CTGATCTTAA GAAGTTTTCT AATTGCATTG TGGGGGCGCT TCAGAAAGTG 
CCAAGGAATG ATAAATCTGA AATTCCATAT TGTGACTTTG CAGACACAGT GTACAAAGAC 
GCCCAACTAA GTGAAATTGA CAAAAACATT TTGGGAAGGA TTGGAATATC CATCATGTAT 
CACTATTACA GAAATAAACA ATCGCAAAAG GGAAAGAGGG TGCTCAGAAA ACTTCAGGAA 
ATGCAGATTA ATTTCACAGT CCTAAAAGGA CTCACAGGGG AAGAGAGTAA AGCTACGCGC 
TGTCAAGTTG TGAACACAGC TGTAGAGATC TTCTTGAACT GTGAATACTT AAATGGTGCA 
CTGGGGGTTC TACGAGAGTC AGAGTGGATC ATTAACACAC AAGTGTGGCC ATGTGAAAGA 
ATGGATGTGC TGAAACGACA TAACCTGTTG TGTACAATTG CACAGAAAAC ACTGAATAAA 
AACATGTTTG ATGTGTGTCT TGAGGTCCTT CAGAATCTGC CAGGACTGCA GTGCTCATTA 
ACGGATGTGG ACGTCTCTCA GTACAGCTTG CTTTTCAACA AACTTTTGTC ATCCTGCATA 
GAAAATAATA CACTTGGTGT ATCCTCAACT GTGATAGATT TTATGATTGC AAAAAAAATA 
CCACTTGACT TTTTCTTACT GAGAGCACTA ATCACATCCT TGGGACGAAG CTGTCTATGG 
CTCAGAGCAA GAGAACTTTA TAAATGCGCT GTTTTGTTGG GTTGCTACCC GCCCATGGAA 
GGAAATCTGT ATCGTAAAGT GCTCTTCATC CCCTCATTCC TTACTGAGAT AGAAATGCTG 
TTATCCATTG AAATTTTCAT GGTGTCAAAT GCCAGTAGTA TTCAGTCACC AGGAGGCAGC 
CATCAGACTC TGCAAATCAT TCTCAAAAGA TGTGAAGAAG AGAGAGTTCC GAATAAAGAT 
TATCAAAGAG GTAAAGCTAG CTATCAAGAT GCAGTGGACC GGCTGATTCA GGCCTCTAGG 
CTATCAACAC CAAGATTATT CATAAAGCAC CTGACTGTCA ATAATGCCAA TGAACAGGTT 
TATATCCTTG ATTACGGCAG CTCTCTCAAA TGGTTGCATG AAAACATGAA ATGGGCTGGG 
AAAGTATGGC TTTTTCAGGG GAAAATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

topaz1 ( XM_012964413.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012819867.1 Xenopus tropicalis testis and ovary specific PAZ domain containing 1 (topaz1), transcript variant X4, mRNA.

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CloneID	OXa33112
Clone ID Related Accession (Same CDS sequence)	XM_012964412.2 , XM_012964413.2 , XM_012964411.2
Accession Version	XM_012964413.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4230bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	testis- and ovary-specific PAZ domain-containing protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012964413.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAAATC CATTGCTTGA AACAGCAAAC AATAGAAAAA GTAAATGTTC ATATGGTGGT 
TCCCAGTCTT TTCTAAATCC TGTAGTGAAA TTGCATAACT GCAGCAACAT GAAGAGGCTT 
ATACGTTGCT CAAATCTGAG GGTAAGCAGT TCCAAAACTG AGAATACATG TCCTGCAGCA 
ACAGTAAAAG AACTTGATCC TCAAAAACAA TTACCTTGTT CTGTTAACAC TGTAAATTCA 
GTTATCAGTA CAGATTCTAC TGTTGGAGGT AATCATTCCA AAGAACTGGA ATCTATTGCA 
CATGGCTTGG CAAATAGGAA AATTAAAAGA ATATTAAAAG TAAAGTGGGA CTTTGGAAAA 
AAAATAAGGA ATTCTGCAAC ATGCTCTACA GAGCGTACTC AAGAAGACTC TGCAATATCT 
GTCAAACAGA AAACAGGAGA CCTAAAATAT AGTGGTAATC CTACTGTCAA CACAGACTCA 
TCAGATCCTT GCATTGGTAC TTTGGAAAAG ATTATTGACG AGATGAACAA TAATAAAAGT 
GACTCTGTAA ATTTTAACTT GAAGGATGCC CTTTGTGATC AAAGCACAAG TCAAAACAAA 
AAGGAAAGTT ATGATTGCCT TCCGGTACAA TGCAGGACTA CACAATTACC GTTTATGGTT 
TCAGAGTGTG AAAGAAAGGA TAGGGAATCC CTTGACATAG AGTGTTCTGC ACTTATTAGA 
ATGTCTGCAA ATGAGTCAGT GGTCGATGAC TATTCAGATT TTTCACTTAC TGAGGAAAAT 
GTTAACTTTG TAAATAATAA CAATATAAAT GAGCATACAG AAGAATGCAA AACCTGTTCC 
TGTCAGAGAA CAATCCCATT TACTGGGAAA ACCATCTGGA AATGTTCTTG TGCAAGGACG 
TGTGTATGGG CTTTCCCAAG AAAAAGAACT TCCTCTTTAC CAAGTACAGA TATACTGGGA 
TTTGACTCTG AATTGTCTTT TGCCCCTTAT TCCACCAATA TAAAGTCTTT ATTTCAGGCT 
AATGAAGCAG AGCCATTGCA GAGCACAACT GATGACACTG ACAGTGACGG GATATGTAAA 
AAACTAAAGT TAGATTTCAT TTCAGAAAAT GACGCTCTTA TTCTTAAAGA TATTGATGAT 
GACATTTTGT CTCACCCTCC AACAGAAACT TTTGAACTAC CCAATACTGT AATTGAGAAG 
ATATGTAATA TAACTGAAAA TGTTTACTCA CTAGGTGAAG TTGAAGACAT GTATAAAAAT 
TTAGATGGTT GGCCATGCAG TTCAACTATT ATTCAAAATA CTATAAATAG TCTTCCTCTC 
CCGCTGCCAA ACAATGACAA AAGTGAGCAC TTTACCACTT CTGGCACTTA TTCTGAAATA 
GAACCTACTG TAAAAACAGT TGAGGAGATG GCCCTGAGCA GTTCTTTTGA AAACTCTGAT 
CCAAGTGTTT GTTCCAGAGA CAGCTCCACA GAACCTGAAA ACAGTTCAGA GACTGTGCTA 
AAAAGTACTC AATATGAATC TCCAATACGT GATTGTTTAG ACAAGCATAA AAAAAACACT 
AATGTTGAGG AAGTGTTATC CTTTTTTGAT TGTTTACATG AAACTACAAT GGATAACGCC 
TGTCTAGCTG AGGAAAATTG CACCAGACAG ATTAATAATA CTGGTGGCCC ATTAGATGAT 
TCACATTTGA CACTAAGCCA TGTAGAATCT CCAAAGAAAA GCAAGCTTGT GGAATACAAA 
AGTAATTTAG ATGTGATAAA AGCTTATGAA GAAGATGTAC TGTTTTTAGA TGTTGTTCCA 
GATGATCCAG AGCTTTTTGG GACTCCTGAA GATCCTTACA TAAGTCCAAT TTGTAATGCT 
GACAACAAAG AAAATAATAA ACGGAATAAT CTGAGTGCAT TAATGTGTAA CCTTCAGGAA 
CAGGAGAGCG CCCATATCAA TGAAAGCACA AATGATGCCA GCCCTGAGGA AAATGTAGAA 
GAGAGAAGTA CATATTTTGC AAAAGTAATC ACTGATCTAG AAGTTAATGA TGATCCTCTC 
ATCATACATA ACACTTTGGA AAAGAACAGT GAAGATTTTT ATGATGGTGG ACAGTTTGAT 
TTTGAGGCGT TCAGAGATCT TGACGCAGAT AAAGAGATTA AATATGCTGA AAAGAATTCA 
TCTGAAAGCG GAAGCAGTTT TGAAGAGTCC TTGTCCAAGG ATACTTCTCC GGGGTCCTCT 
CATTCCAATA GCCGTCGTTG GAGAAGAGAT CCCTCACATA CCTATAAGCC TATGCATTGG 
ATGAATGATT TCAGATACTC TGGAAAATGC ATGGGATTGA CGGATGTGAT TACTTTGGAT 
CAGAAAGAAC AGAGGAATAT TGATAATCTC TTTTACGATT TTACACCTAT ACAGAAGACC 
TTTTTGCCCA TTAGATACTG CAAATATTTT TTTAATACTT TCCGTGGATG CATTAAACCA 
GATTGTATGT ACCAGCACGT ACCATTCCAA AGGGATGAAA AGGTGTGCAT GGAGGTGATT 
CACAAACTTG TGAATGAAAA TCACACAACA TTATTAAAAC GTGCTGTTTG GATATTTACT 
GCCTACTACA GAATGTATAT GCCTGGTGTT CATTATGACT CAAATCTGCT GACCAAAATG 
CTCCGTGCCT TGTATGTTCG TCAGATGTGG GGAGATGTAT TTCAGTTGTT GGAAACTGGT 
GCCAATGTCA AAATATTGCC CTCCTCTGAA ATGCTTATCA GGTTATTTGA AAATATTGGC 
TCTACGGGAT TAACAGCAGC TGTACCTAGT TTAGTGGATG TATTCTGCAA GCTTGTGGAG 
GCTGGAATGA TGATGAAGCC AGAGCAAATC AACATGTTAA TAACAACACT GAATAATCTC 
CAGGCAACAA AAAATTATAT CAGTGTCATT TTAGACATAA AAACAAGAAT TGAAATGCAG 
CTTTCAGAAA AGAACTGGCT GTGTAACTTG GATATAGCAG TTGCAGAAGT TGGGCATTGT 
AAAGAAAAAA ATGATTGGAA GAAACTAGGA ACATTATATC TTAATCTGCG TACAGGGTGT 
GAGAATGTGA CTGATCTTAA GAAGTTTTCT AATTGCATTG TGGGGGCGCT TCAGAAAGTG 
CCAAGGAATG ATAAATCTGA AATTCCATAT TGTGACTTTG CAGACACAGT GTACAAAGAC 
GCCCAACTAA GTGAAATTGA CAAAAACATT TTGGGAAGGA TTGGAATATC CATCATGTAT 
CACTATTACA GAAATAAACA ATCGCAAAAG GGAAAGAGGG TGCTCAGAAA ACTTCAGGAA 
ATGCAGATTA ATTTCACAGT CCTAAAAGGA CTCACAGGGG AAGAGAGTAA AGCTACGCGC 
TGTCAAGTTG TGAACACAGC TGTAGAGATC TTCTTGAACT GTGAATACTT AAATGGTGCA 
CTGGGGGTTC TACGAGAGTC AGAGTGGATC ATTAACACAC AAGTGTGGCC ATGTGAAAGA 
ATGGATGTGC TGAAACGACA TAACCTGTTG TGTACAATTG CACAGAAAAC ACTGAATAAA 
AACATGTTTG ATGTGTGTCT TGAGGTCCTT CAGAATCTGC CAGGACTGCA GTGCTCATTA 
ACGGATGTGG ACGTCTCTCA GTACAGCTTG CTTTTCAACA AACTTTTGTC ATCCTGCATA 
GAAAATAATA CACTTGGTGT ATCCTCAACT GTGATAGATT TTATGATTGC AAAAAAAATA 
CCACTTGACT TTTTCTTACT GAGAGCACTA ATCACATCCT TGGGACGAAG CTGTCTATGG 
CTCAGAGCAA GAGAACTTTA TAAATGCGCT GTTTTGTTGG GTTGCTACCC GCCCATGGAA 
GGAAATCTGT ATCGTAAAGT GCTCTTCATC CCCTCATTCC TTACTGAGAT AGAAATGCTG 
TTATCCATTG AAATTTTCAT GGTGTCAAAT GCCAGTAGTA TTCAGTCACC AGGAGGCAGC 
CATCAGACTC TGCAAATCAT TCTCAAAAGA TGTGAAGAAG AGAGAGTTCC GAATAAAGAT 
TATCAAAGAG GTAAAGCTAG CTATCAAGAT GCAGTGGACC GGCTGATTCA GGCCTCTAGG 
CTATCAACAC CAAGATTATT CATAAAGCAC CTGACTGTCA ATAATGCCAA TGAACAGGTT 
TATATCCTTG ATTACGGCAG CTCTCTCAAA TGGTTGCATG AAAACATGAA ATGGGCTGGG 
AAAGTATGGC TTTTTCAGGG GAAAATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012819867.1
CDS	219..4448
Translation	MQNPLLETANNRKSKCSYGGSQSFLNPVVKLHNCSNMKRLIRCSNLRVSSSKTENTCPAATVKELDPQKQLPCSVNTVNSVISTDSTVGGNHSKELESIAHGLANRKIKRILKVKWDFGKKIRNSATCSTERTQEDSAISVKQKTGDLKYSGNPTVNTDSSDPCIGTLEKIIDEMNNNKSDSVNFNLKDALCDQSTSQNKKESYDCLPVQCRTTQLPFMVSECERKDRESLDIECSALIRMSANESVVDDYSDFSLTEENVNFVNNNNINEHTEECKTCSCQRTIPFTGKTIWKCSCARTCVWAFPRKRTSSLPSTDILGFDSELSFAPYSTNIKSLFQANEAEPLQSTTDDTDSDGICKKLKLDFISENDALILKDIDDDILSHPPTETFELPNTVIEKICNITENVYSLGEVEDMYKNLDGWPCSSTIIQNTINSLPLPLPNNDKSEHFTTSGTYSEIEPTVKTVEEMALSSSFENSDPSVCSRDSSTEPENSSETVLKSTQYESPIRDCLDKHKKNTNVEEVLSFFDCLHETTMDNACLAEENCTRQINNTGGPLDDSHLTLSHVESPKKSKLVEYKSNLDVIKAYEEDVLFLDVVPDDPELFGTPEDPYISPICNADNKENNKRNNLSALMCNLQEQESAHINESTNDASPEENVEERSTYFAKVITDLEVNDDPLIIHNTLEKNSEDFYDGGQFDFEAFRDLDADKEIKYAEKNSSESGSSFEESLSKDTSPGSSHSNSRRWRRDPSHTYKPMHWMNDFRYSGKCMGLTDVITLDQKEQRNIDNLFYDFTPIQKTFLPIRYCKYFFNTFRGCIKPDCMYQHVPFQRDEKVCMEVIHKLVNENHTTLLKRAVWIFTAYYRMYMPGVHYDSNLLTKMLRALYVRQMWGDVFQLLETGANVKILPSSEMLIRLFENIGSTGLTAAVPSLVDVFCKLVEAGMMMKPEQINMLITTLNNLQATKNYISVILDIKTRIEMQLSEKNWLCNLDIAVAEVGHCKEKNDWKKLGTLYLNLRTGCENVTDLKKFSNCIVGALQKVPRNDKSEIPYCDFADTVYKDAQLSEIDKNILGRIGISIMYHYYRNKQSQKGKRVLRKLQEMQINFTVLKGLTGEESKATRCQVVNTAVEIFLNCEYLNGALGVLRESEWIINTQVWPCERMDVLKRHNLLCTIAQKTLNKNMFDVCLEVLQNLPGLQCSLTDVDVSQYSLLFNKLLSSCIENNTLGVSSTVIDFMIAKKIPLDFFLLRALITSLGRSCLWLRARELYKCAVLLGCYPPMEGNLYRKVLFIPSFLTEIEMLLSIEIFMVSNASSIQSPGGSHQTLQIILKRCEEERVPNKDYQRGKASYQDAVDRLIQASRLSTPRLFIKHLTVNNANEQVYILDYGSSLKWLHENMKWAGKVWLFQGKI

Target ORF information:

RefSeq Version	XM_012964413.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis testis and ovary specific PAZ domain containing 1 (topaz1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012964413.2

ORF Insert Sequence:

ATGCAAAATC CATTGCTTGA AACAGCAAAC AATAGAAAAA GTAAATGTTC ATATGGTGGT 
TCCCAGTCTT TTCTAAATCC TGTAGTGAAA TTGCATAACT GCAGCAACAT GAAGAGGCTT 
ATACGTTGCT CAAATCTGAG GGTAAGCAGT TCCAAAACTG AGAATACATG TCCTGCAGCA 
ACAGTAAAAG AACTTGATCC TCAAAAACAA TTACCTTGTT CTGTTAACAC TGTAAATTCA 
GTTATCAGTA CAGATTCTAC TGTTGGAGGT AATCATTCCA AAGAACTGGA ATCTATTGCA 
CATGGCTTGG CAAATAGGAA AATTAAAAGA ATATTAAAAG TAAAGTGGGA CTTTGGAAAA 
AAAATAAGGA ATTCTGCAAC ATGCTCTACA GAGCGTACTC AAGAAGACTC TGCAATATCT 
GTCAAACAGA AAACAGGAGA CCTAAAATAT AGTGGTAATC CTACTGTCAA CACAGACTCA 
TCAGATCCTT GCATTGGTAC TTTGGAAAAG ATTATTGACG AGATGAACAA TAATAAAAGT 
GACTCTGTAA ATTTTAACTT GAAGGATGCC CTTTGTGATC AAAGCACAAG TCAAAACAAA 
AAGGAAAGTT ATGATTGCCT TCCGGTACAA TGCAGGACTA CACAATTACC GTTTATGGTT 
TCAGAGTGTG AAAGAAAGGA TAGGGAATCC CTTGACATAG AGTGTTCTGC ACTTATTAGA 
ATGTCTGCAA ATGAGTCAGT GGTCGATGAC TATTCAGATT TTTCACTTAC TGAGGAAAAT 
GTTAACTTTG TAAATAATAA CAATATAAAT GAGCATACAG AAGAATGCAA AACCTGTTCC 
TGTCAGAGAA CAATCCCATT TACTGGGAAA ACCATCTGGA AATGTTCTTG TGCAAGGACG 
TGTGTATGGG CTTTCCCAAG AAAAAGAACT TCCTCTTTAC CAAGTACAGA TATACTGGGA 
TTTGACTCTG AATTGTCTTT TGCCCCTTAT TCCACCAATA TAAAGTCTTT ATTTCAGGCT 
AATGAAGCAG AGCCATTGCA GAGCACAACT GATGACACTG ACAGTGACGG GATATGTAAA 
AAACTAAAGT TAGATTTCAT TTCAGAAAAT GACGCTCTTA TTCTTAAAGA TATTGATGAT 
GACATTTTGT CTCACCCTCC AACAGAAACT TTTGAACTAC CCAATACTGT AATTGAGAAG 
ATATGTAATA TAACTGAAAA TGTTTACTCA CTAGGTGAAG TTGAAGACAT GTATAAAAAT 
TTAGATGGTT GGCCATGCAG TTCAACTATT ATTCAAAATA CTATAAATAG TCTTCCTCTC 
CCGCTGCCAA ACAATGACAA AAGTGAGCAC TTTACCACTT CTGGCACTTA TTCTGAAATA 
GAACCTACTG TAAAAACAGT TGAGGAGATG GCCCTGAGCA GTTCTTTTGA AAACTCTGAT 
CCAAGTGTTT GTTCCAGAGA CAGCTCCACA GAACCTGAAA ACAGTTCAGA GACTGTGCTA 
AAAAGTACTC AATATGAATC TCCAATACGT GATTGTTTAG ACAAGCATAA AAAAAACACT 
AATGTTGAGG AAGTGTTATC CTTTTTTGAT TGTTTACATG AAACTACAAT GGATAACGCC 
TGTCTAGCTG AGGAAAATTG CACCAGACAG ATTAATAATA CTGGTGGCCC ATTAGATGAT 
TCACATTTGA CACTAAGCCA TGTAGAATCT CCAAAGAAAA GCAAGCTTGT GGAATACAAA 
AGTAATTTAG ATGTGATAAA AGCTTATGAA GAAGATGTAC TGTTTTTAGA TGTTGTTCCA 
GATGATCCAG AGCTTTTTGG GACTCCTGAA GATCCTTACA TAAGTCCAAT TTGTAATGCT 
GACAACAAAG AAAATAATAA ACGGAATAAT CTGAGTGCAT TAATGTGTAA CCTTCAGGAA 
CAGGAGAGCG CCCATATCAA TGAAAGCACA AATGATGCCA GCCCTGAGGA AAATGTAGAA 
GAGAGAAGTA CATATTTTGC AAAAGTAATC ACTGATCTAG AAGTTAATGA TGATCCTCTC 
ATCATACATA ACACTTTGGA AAAGAACAGT GAAGATTTTT ATGATGGTGG ACAGTTTGAT 
TTTGAGGCGT TCAGAGATCT TGACGCAGAT AAAGAGATTA AATATGCTGA AAAGAATTCA 
TCTGAAAGCG GAAGCAGTTT TGAAGAGTCC TTGTCCAAGG ATACTTCTCC GGGGTCCTCT 
CATTCCAATA GCCGTCGTTG GAGAAGAGAT CCCTCACATA CCTATAAGCC TATGCATTGG 
ATGAATGATT TCAGATACTC TGGAAAATGC ATGGGATTGA CGGATGTGAT TACTTTGGAT 
CAGAAAGAAC AGAGGAATAT TGATAATCTC TTTTACGATT TTACACCTAT ACAGAAGACC 
TTTTTGCCCA TTAGATACTG CAAATATTTT TTTAATACTT TCCGTGGATG CATTAAACCA 
GATTGTATGT ACCAGCACGT ACCATTCCAA AGGGATGAAA AGGTGTGCAT GGAGGTGATT 
CACAAACTTG TGAATGAAAA TCACACAACA TTATTAAAAC GTGCTGTTTG GATATTTACT 
GCCTACTACA GAATGTATAT GCCTGGTGTT CATTATGACT CAAATCTGCT GACCAAAATG 
CTCCGTGCCT TGTATGTTCG TCAGATGTGG GGAGATGTAT TTCAGTTGTT GGAAACTGGT 
GCCAATGTCA AAATATTGCC CTCCTCTGAA ATGCTTATCA GGTTATTTGA AAATATTGGC 
TCTACGGGAT TAACAGCAGC TGTACCTAGT TTAGTGGATG TATTCTGCAA GCTTGTGGAG 
GCTGGAATGA TGATGAAGCC AGAGCAAATC AACATGTTAA TAACAACACT GAATAATCTC 
CAGGCAACAA AAAATTATAT CAGTGTCATT TTAGACATAA AAACAAGAAT TGAAATGCAG 
CTTTCAGAAA AGAACTGGCT GTGTAACTTG GATATAGCAG TTGCAGAAGT TGGGCATTGT 
AAAGAAAAAA ATGATTGGAA GAAACTAGGA ACATTATATC TTAATCTGCG TACAGGGTGT 
GAGAATGTGA CTGATCTTAA GAAGTTTTCT AATTGCATTG TGGGGGCGCT TCAGAAAGTG 
CCAAGGAATG ATAAATCTGA AATTCCATAT TGTGACTTTG CAGACACAGT GTACAAAGAC 
GCCCAACTAA GTGAAATTGA CAAAAACATT TTGGGAAGGA TTGGAATATC CATCATGTAT 
CACTATTACA GAAATAAACA ATCGCAAAAG GGAAAGAGGG TGCTCAGAAA ACTTCAGGAA 
ATGCAGATTA ATTTCACAGT CCTAAAAGGA CTCACAGGGG AAGAGAGTAA AGCTACGCGC 
TGTCAAGTTG TGAACACAGC TGTAGAGATC TTCTTGAACT GTGAATACTT AAATGGTGCA 
CTGGGGGTTC TACGAGAGTC AGAGTGGATC ATTAACACAC AAGTGTGGCC ATGTGAAAGA 
ATGGATGTGC TGAAACGACA TAACCTGTTG TGTACAATTG CACAGAAAAC ACTGAATAAA 
AACATGTTTG ATGTGTGTCT TGAGGTCCTT CAGAATCTGC CAGGACTGCA GTGCTCATTA 
ACGGATGTGG ACGTCTCTCA GTACAGCTTG CTTTTCAACA AACTTTTGTC ATCCTGCATA 
GAAAATAATA CACTTGGTGT ATCCTCAACT GTGATAGATT TTATGATTGC AAAAAAAATA 
CCACTTGACT TTTTCTTACT GAGAGCACTA ATCACATCCT TGGGACGAAG CTGTCTATGG 
CTCAGAGCAA GAGAACTTTA TAAATGCGCT GTTTTGTTGG GTTGCTACCC GCCCATGGAA 
GGAAATCTGT ATCGTAAAGT GCTCTTCATC CCCTCATTCC TTACTGAGAT AGAAATGCTG 
TTATCCATTG AAATTTTCAT GGTGTCAAAT GCCAGTAGTA TTCAGTCACC AGGAGGCAGC 
CATCAGACTC TGCAAATCAT TCTCAAAAGA TGTGAAGAAG AGAGAGTTCC GAATAAAGAT 
TATCAAAGAG GTAAAGCTAG CTATCAAGAT GCAGTGGACC GGCTGATTCA GGCCTCTAGG 
CTATCAACAC CAAGATTATT CATAAAGCAC CTGACTGTCA ATAATGCCAA TGAACAGGTT 
TATATCCTTG ATTACGGCAG CTCTCTCAAA TGGTTGCATG AAAACATGAA ATGGGCTGGG 
AAAGTATGGC TTTTTCAGGG GAAAATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

topaz1 ( XM_012964411.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012819865.1 Xenopus tropicalis testis and ovary specific PAZ domain containing 1 (topaz1), transcript variant X3, mRNA.

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CloneID	OXa33112
Clone ID Related Accession (Same CDS sequence)	XM_012964412.2 , XM_012964413.2 , XM_012964411.2
Accession Version	XM_012964411.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4230bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	testis- and ovary-specific PAZ domain-containing protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012964411.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAAATC CATTGCTTGA AACAGCAAAC AATAGAAAAA GTAAATGTTC ATATGGTGGT 
TCCCAGTCTT TTCTAAATCC TGTAGTGAAA TTGCATAACT GCAGCAACAT GAAGAGGCTT 
ATACGTTGCT CAAATCTGAG GGTAAGCAGT TCCAAAACTG AGAATACATG TCCTGCAGCA 
ACAGTAAAAG AACTTGATCC TCAAAAACAA TTACCTTGTT CTGTTAACAC TGTAAATTCA 
GTTATCAGTA CAGATTCTAC TGTTGGAGGT AATCATTCCA AAGAACTGGA ATCTATTGCA 
CATGGCTTGG CAAATAGGAA AATTAAAAGA ATATTAAAAG TAAAGTGGGA CTTTGGAAAA 
AAAATAAGGA ATTCTGCAAC ATGCTCTACA GAGCGTACTC AAGAAGACTC TGCAATATCT 
GTCAAACAGA AAACAGGAGA CCTAAAATAT AGTGGTAATC CTACTGTCAA CACAGACTCA 
TCAGATCCTT GCATTGGTAC TTTGGAAAAG ATTATTGACG AGATGAACAA TAATAAAAGT 
GACTCTGTAA ATTTTAACTT GAAGGATGCC CTTTGTGATC AAAGCACAAG TCAAAACAAA 
AAGGAAAGTT ATGATTGCCT TCCGGTACAA TGCAGGACTA CACAATTACC GTTTATGGTT 
TCAGAGTGTG AAAGAAAGGA TAGGGAATCC CTTGACATAG AGTGTTCTGC ACTTATTAGA 
ATGTCTGCAA ATGAGTCAGT GGTCGATGAC TATTCAGATT TTTCACTTAC TGAGGAAAAT 
GTTAACTTTG TAAATAATAA CAATATAAAT GAGCATACAG AAGAATGCAA AACCTGTTCC 
TGTCAGAGAA CAATCCCATT TACTGGGAAA ACCATCTGGA AATGTTCTTG TGCAAGGACG 
TGTGTATGGG CTTTCCCAAG AAAAAGAACT TCCTCTTTAC CAAGTACAGA TATACTGGGA 
TTTGACTCTG AATTGTCTTT TGCCCCTTAT TCCACCAATA TAAAGTCTTT ATTTCAGGCT 
AATGAAGCAG AGCCATTGCA GAGCACAACT GATGACACTG ACAGTGACGG GATATGTAAA 
AAACTAAAGT TAGATTTCAT TTCAGAAAAT GACGCTCTTA TTCTTAAAGA TATTGATGAT 
GACATTTTGT CTCACCCTCC AACAGAAACT TTTGAACTAC CCAATACTGT AATTGAGAAG 
ATATGTAATA TAACTGAAAA TGTTTACTCA CTAGGTGAAG TTGAAGACAT GTATAAAAAT 
TTAGATGGTT GGCCATGCAG TTCAACTATT ATTCAAAATA CTATAAATAG TCTTCCTCTC 
CCGCTGCCAA ACAATGACAA AAGTGAGCAC TTTACCACTT CTGGCACTTA TTCTGAAATA 
GAACCTACTG TAAAAACAGT TGAGGAGATG GCCCTGAGCA GTTCTTTTGA AAACTCTGAT 
CCAAGTGTTT GTTCCAGAGA CAGCTCCACA GAACCTGAAA ACAGTTCAGA GACTGTGCTA 
AAAAGTACTC AATATGAATC TCCAATACGT GATTGTTTAG ACAAGCATAA AAAAAACACT 
AATGTTGAGG AAGTGTTATC CTTTTTTGAT TGTTTACATG AAACTACAAT GGATAACGCC 
TGTCTAGCTG AGGAAAATTG CACCAGACAG ATTAATAATA CTGGTGGCCC ATTAGATGAT 
TCACATTTGA CACTAAGCCA TGTAGAATCT CCAAAGAAAA GCAAGCTTGT GGAATACAAA 
AGTAATTTAG ATGTGATAAA AGCTTATGAA GAAGATGTAC TGTTTTTAGA TGTTGTTCCA 
GATGATCCAG AGCTTTTTGG GACTCCTGAA GATCCTTACA TAAGTCCAAT TTGTAATGCT 
GACAACAAAG AAAATAATAA ACGGAATAAT CTGAGTGCAT TAATGTGTAA CCTTCAGGAA 
CAGGAGAGCG CCCATATCAA TGAAAGCACA AATGATGCCA GCCCTGAGGA AAATGTAGAA 
GAGAGAAGTA CATATTTTGC AAAAGTAATC ACTGATCTAG AAGTTAATGA TGATCCTCTC 
ATCATACATA ACACTTTGGA AAAGAACAGT GAAGATTTTT ATGATGGTGG ACAGTTTGAT 
TTTGAGGCGT TCAGAGATCT TGACGCAGAT AAAGAGATTA AATATGCTGA AAAGAATTCA 
TCTGAAAGCG GAAGCAGTTT TGAAGAGTCC TTGTCCAAGG ATACTTCTCC GGGGTCCTCT 
CATTCCAATA GCCGTCGTTG GAGAAGAGAT CCCTCACATA CCTATAAGCC TATGCATTGG 
ATGAATGATT TCAGATACTC TGGAAAATGC ATGGGATTGA CGGATGTGAT TACTTTGGAT 
CAGAAAGAAC AGAGGAATAT TGATAATCTC TTTTACGATT TTACACCTAT ACAGAAGACC 
TTTTTGCCCA TTAGATACTG CAAATATTTT TTTAATACTT TCCGTGGATG CATTAAACCA 
GATTGTATGT ACCAGCACGT ACCATTCCAA AGGGATGAAA AGGTGTGCAT GGAGGTGATT 
CACAAACTTG TGAATGAAAA TCACACAACA TTATTAAAAC GTGCTGTTTG GATATTTACT 
GCCTACTACA GAATGTATAT GCCTGGTGTT CATTATGACT CAAATCTGCT GACCAAAATG 
CTCCGTGCCT TGTATGTTCG TCAGATGTGG GGAGATGTAT TTCAGTTGTT GGAAACTGGT 
GCCAATGTCA AAATATTGCC CTCCTCTGAA ATGCTTATCA GGTTATTTGA AAATATTGGC 
TCTACGGGAT TAACAGCAGC TGTACCTAGT TTAGTGGATG TATTCTGCAA GCTTGTGGAG 
GCTGGAATGA TGATGAAGCC AGAGCAAATC AACATGTTAA TAACAACACT GAATAATCTC 
CAGGCAACAA AAAATTATAT CAGTGTCATT TTAGACATAA AAACAAGAAT TGAAATGCAG 
CTTTCAGAAA AGAACTGGCT GTGTAACTTG GATATAGCAG TTGCAGAAGT TGGGCATTGT 
AAAGAAAAAA ATGATTGGAA GAAACTAGGA ACATTATATC TTAATCTGCG TACAGGGTGT 
GAGAATGTGA CTGATCTTAA GAAGTTTTCT AATTGCATTG TGGGGGCGCT TCAGAAAGTG 
CCAAGGAATG ATAAATCTGA AATTCCATAT TGTGACTTTG CAGACACAGT GTACAAAGAC 
GCCCAACTAA GTGAAATTGA CAAAAACATT TTGGGAAGGA TTGGAATATC CATCATGTAT 
CACTATTACA GAAATAAACA ATCGCAAAAG GGAAAGAGGG TGCTCAGAAA ACTTCAGGAA 
ATGCAGATTA ATTTCACAGT CCTAAAAGGA CTCACAGGGG AAGAGAGTAA AGCTACGCGC 
TGTCAAGTTG TGAACACAGC TGTAGAGATC TTCTTGAACT GTGAATACTT AAATGGTGCA 
CTGGGGGTTC TACGAGAGTC AGAGTGGATC ATTAACACAC AAGTGTGGCC ATGTGAAAGA 
ATGGATGTGC TGAAACGACA TAACCTGTTG TGTACAATTG CACAGAAAAC ACTGAATAAA 
AACATGTTTG ATGTGTGTCT TGAGGTCCTT CAGAATCTGC CAGGACTGCA GTGCTCATTA 
ACGGATGTGG ACGTCTCTCA GTACAGCTTG CTTTTCAACA AACTTTTGTC ATCCTGCATA 
GAAAATAATA CACTTGGTGT ATCCTCAACT GTGATAGATT TTATGATTGC AAAAAAAATA 
CCACTTGACT TTTTCTTACT GAGAGCACTA ATCACATCCT TGGGACGAAG CTGTCTATGG 
CTCAGAGCAA GAGAACTTTA TAAATGCGCT GTTTTGTTGG GTTGCTACCC GCCCATGGAA 
GGAAATCTGT ATCGTAAAGT GCTCTTCATC CCCTCATTCC TTACTGAGAT AGAAATGCTG 
TTATCCATTG AAATTTTCAT GGTGTCAAAT GCCAGTAGTA TTCAGTCACC AGGAGGCAGC 
CATCAGACTC TGCAAATCAT TCTCAAAAGA TGTGAAGAAG AGAGAGTTCC GAATAAAGAT 
TATCAAAGAG GTAAAGCTAG CTATCAAGAT GCAGTGGACC GGCTGATTCA GGCCTCTAGG 
CTATCAACAC CAAGATTATT CATAAAGCAC CTGACTGTCA ATAATGCCAA TGAACAGGTT 
TATATCCTTG ATTACGGCAG CTCTCTCAAA TGGTTGCATG AAAACATGAA ATGGGCTGGG 
AAAGTATGGC TTTTTCAGGG GAAAATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012819865.1
CDS	298..4527
Translation	MQNPLLETANNRKSKCSYGGSQSFLNPVVKLHNCSNMKRLIRCSNLRVSSSKTENTCPAATVKELDPQKQLPCSVNTVNSVISTDSTVGGNHSKELESIAHGLANRKIKRILKVKWDFGKKIRNSATCSTERTQEDSAISVKQKTGDLKYSGNPTVNTDSSDPCIGTLEKIIDEMNNNKSDSVNFNLKDALCDQSTSQNKKESYDCLPVQCRTTQLPFMVSECERKDRESLDIECSALIRMSANESVVDDYSDFSLTEENVNFVNNNNINEHTEECKTCSCQRTIPFTGKTIWKCSCARTCVWAFPRKRTSSLPSTDILGFDSELSFAPYSTNIKSLFQANEAEPLQSTTDDTDSDGICKKLKLDFISENDALILKDIDDDILSHPPTETFELPNTVIEKICNITENVYSLGEVEDMYKNLDGWPCSSTIIQNTINSLPLPLPNNDKSEHFTTSGTYSEIEPTVKTVEEMALSSSFENSDPSVCSRDSSTEPENSSETVLKSTQYESPIRDCLDKHKKNTNVEEVLSFFDCLHETTMDNACLAEENCTRQINNTGGPLDDSHLTLSHVESPKKSKLVEYKSNLDVIKAYEEDVLFLDVVPDDPELFGTPEDPYISPICNADNKENNKRNNLSALMCNLQEQESAHINESTNDASPEENVEERSTYFAKVITDLEVNDDPLIIHNTLEKNSEDFYDGGQFDFEAFRDLDADKEIKYAEKNSSESGSSFEESLSKDTSPGSSHSNSRRWRRDPSHTYKPMHWMNDFRYSGKCMGLTDVITLDQKEQRNIDNLFYDFTPIQKTFLPIRYCKYFFNTFRGCIKPDCMYQHVPFQRDEKVCMEVIHKLVNENHTTLLKRAVWIFTAYYRMYMPGVHYDSNLLTKMLRALYVRQMWGDVFQLLETGANVKILPSSEMLIRLFENIGSTGLTAAVPSLVDVFCKLVEAGMMMKPEQINMLITTLNNLQATKNYISVILDIKTRIEMQLSEKNWLCNLDIAVAEVGHCKEKNDWKKLGTLYLNLRTGCENVTDLKKFSNCIVGALQKVPRNDKSEIPYCDFADTVYKDAQLSEIDKNILGRIGISIMYHYYRNKQSQKGKRVLRKLQEMQINFTVLKGLTGEESKATRCQVVNTAVEIFLNCEYLNGALGVLRESEWIINTQVWPCERMDVLKRHNLLCTIAQKTLNKNMFDVCLEVLQNLPGLQCSLTDVDVSQYSLLFNKLLSSCIENNTLGVSSTVIDFMIAKKIPLDFFLLRALITSLGRSCLWLRARELYKCAVLLGCYPPMEGNLYRKVLFIPSFLTEIEMLLSIEIFMVSNASSIQSPGGSHQTLQIILKRCEEERVPNKDYQRGKASYQDAVDRLIQASRLSTPRLFIKHLTVNNANEQVYILDYGSSLKWLHENMKWAGKVWLFQGKI

Target ORF information:

RefSeq Version	XM_012964411.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis testis and ovary specific PAZ domain containing 1 (topaz1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012964411.2

ORF Insert Sequence:

ATGCAAAATC CATTGCTTGA AACAGCAAAC AATAGAAAAA GTAAATGTTC ATATGGTGGT 
TCCCAGTCTT TTCTAAATCC TGTAGTGAAA TTGCATAACT GCAGCAACAT GAAGAGGCTT 
ATACGTTGCT CAAATCTGAG GGTAAGCAGT TCCAAAACTG AGAATACATG TCCTGCAGCA 
ACAGTAAAAG AACTTGATCC TCAAAAACAA TTACCTTGTT CTGTTAACAC TGTAAATTCA 
GTTATCAGTA CAGATTCTAC TGTTGGAGGT AATCATTCCA AAGAACTGGA ATCTATTGCA 
CATGGCTTGG CAAATAGGAA AATTAAAAGA ATATTAAAAG TAAAGTGGGA CTTTGGAAAA 
AAAATAAGGA ATTCTGCAAC ATGCTCTACA GAGCGTACTC AAGAAGACTC TGCAATATCT 
GTCAAACAGA AAACAGGAGA CCTAAAATAT AGTGGTAATC CTACTGTCAA CACAGACTCA 
TCAGATCCTT GCATTGGTAC TTTGGAAAAG ATTATTGACG AGATGAACAA TAATAAAAGT 
GACTCTGTAA ATTTTAACTT GAAGGATGCC CTTTGTGATC AAAGCACAAG TCAAAACAAA 
AAGGAAAGTT ATGATTGCCT TCCGGTACAA TGCAGGACTA CACAATTACC GTTTATGGTT 
TCAGAGTGTG AAAGAAAGGA TAGGGAATCC CTTGACATAG AGTGTTCTGC ACTTATTAGA 
ATGTCTGCAA ATGAGTCAGT GGTCGATGAC TATTCAGATT TTTCACTTAC TGAGGAAAAT 
GTTAACTTTG TAAATAATAA CAATATAAAT GAGCATACAG AAGAATGCAA AACCTGTTCC 
TGTCAGAGAA CAATCCCATT TACTGGGAAA ACCATCTGGA AATGTTCTTG TGCAAGGACG 
TGTGTATGGG CTTTCCCAAG AAAAAGAACT TCCTCTTTAC CAAGTACAGA TATACTGGGA 
TTTGACTCTG AATTGTCTTT TGCCCCTTAT TCCACCAATA TAAAGTCTTT ATTTCAGGCT 
AATGAAGCAG AGCCATTGCA GAGCACAACT GATGACACTG ACAGTGACGG GATATGTAAA 
AAACTAAAGT TAGATTTCAT TTCAGAAAAT GACGCTCTTA TTCTTAAAGA TATTGATGAT 
GACATTTTGT CTCACCCTCC AACAGAAACT TTTGAACTAC CCAATACTGT AATTGAGAAG 
ATATGTAATA TAACTGAAAA TGTTTACTCA CTAGGTGAAG TTGAAGACAT GTATAAAAAT 
TTAGATGGTT GGCCATGCAG TTCAACTATT ATTCAAAATA CTATAAATAG TCTTCCTCTC 
CCGCTGCCAA ACAATGACAA AAGTGAGCAC TTTACCACTT CTGGCACTTA TTCTGAAATA 
GAACCTACTG TAAAAACAGT TGAGGAGATG GCCCTGAGCA GTTCTTTTGA AAACTCTGAT 
CCAAGTGTTT GTTCCAGAGA CAGCTCCACA GAACCTGAAA ACAGTTCAGA GACTGTGCTA 
AAAAGTACTC AATATGAATC TCCAATACGT GATTGTTTAG ACAAGCATAA AAAAAACACT 
AATGTTGAGG AAGTGTTATC CTTTTTTGAT TGTTTACATG AAACTACAAT GGATAACGCC 
TGTCTAGCTG AGGAAAATTG CACCAGACAG ATTAATAATA CTGGTGGCCC ATTAGATGAT 
TCACATTTGA CACTAAGCCA TGTAGAATCT CCAAAGAAAA GCAAGCTTGT GGAATACAAA 
AGTAATTTAG ATGTGATAAA AGCTTATGAA GAAGATGTAC TGTTTTTAGA TGTTGTTCCA 
GATGATCCAG AGCTTTTTGG GACTCCTGAA GATCCTTACA TAAGTCCAAT TTGTAATGCT 
GACAACAAAG AAAATAATAA ACGGAATAAT CTGAGTGCAT TAATGTGTAA CCTTCAGGAA 
CAGGAGAGCG CCCATATCAA TGAAAGCACA AATGATGCCA GCCCTGAGGA AAATGTAGAA 
GAGAGAAGTA CATATTTTGC AAAAGTAATC ACTGATCTAG AAGTTAATGA TGATCCTCTC 
ATCATACATA ACACTTTGGA AAAGAACAGT GAAGATTTTT ATGATGGTGG ACAGTTTGAT 
TTTGAGGCGT TCAGAGATCT TGACGCAGAT AAAGAGATTA AATATGCTGA AAAGAATTCA 
TCTGAAAGCG GAAGCAGTTT TGAAGAGTCC TTGTCCAAGG ATACTTCTCC GGGGTCCTCT 
CATTCCAATA GCCGTCGTTG GAGAAGAGAT CCCTCACATA CCTATAAGCC TATGCATTGG 
ATGAATGATT TCAGATACTC TGGAAAATGC ATGGGATTGA CGGATGTGAT TACTTTGGAT 
CAGAAAGAAC AGAGGAATAT TGATAATCTC TTTTACGATT TTACACCTAT ACAGAAGACC 
TTTTTGCCCA TTAGATACTG CAAATATTTT TTTAATACTT TCCGTGGATG CATTAAACCA 
GATTGTATGT ACCAGCACGT ACCATTCCAA AGGGATGAAA AGGTGTGCAT GGAGGTGATT 
CACAAACTTG TGAATGAAAA TCACACAACA TTATTAAAAC GTGCTGTTTG GATATTTACT 
GCCTACTACA GAATGTATAT GCCTGGTGTT CATTATGACT CAAATCTGCT GACCAAAATG 
CTCCGTGCCT TGTATGTTCG TCAGATGTGG GGAGATGTAT TTCAGTTGTT GGAAACTGGT 
GCCAATGTCA AAATATTGCC CTCCTCTGAA ATGCTTATCA GGTTATTTGA AAATATTGGC 
TCTACGGGAT TAACAGCAGC TGTACCTAGT TTAGTGGATG TATTCTGCAA GCTTGTGGAG 
GCTGGAATGA TGATGAAGCC AGAGCAAATC AACATGTTAA TAACAACACT GAATAATCTC 
CAGGCAACAA AAAATTATAT CAGTGTCATT TTAGACATAA AAACAAGAAT TGAAATGCAG 
CTTTCAGAAA AGAACTGGCT GTGTAACTTG GATATAGCAG TTGCAGAAGT TGGGCATTGT 
AAAGAAAAAA ATGATTGGAA GAAACTAGGA ACATTATATC TTAATCTGCG TACAGGGTGT 
GAGAATGTGA CTGATCTTAA GAAGTTTTCT AATTGCATTG TGGGGGCGCT TCAGAAAGTG 
CCAAGGAATG ATAAATCTGA AATTCCATAT TGTGACTTTG CAGACACAGT GTACAAAGAC 
GCCCAACTAA GTGAAATTGA CAAAAACATT TTGGGAAGGA TTGGAATATC CATCATGTAT 
CACTATTACA GAAATAAACA ATCGCAAAAG GGAAAGAGGG TGCTCAGAAA ACTTCAGGAA 
ATGCAGATTA ATTTCACAGT CCTAAAAGGA CTCACAGGGG AAGAGAGTAA AGCTACGCGC 
TGTCAAGTTG TGAACACAGC TGTAGAGATC TTCTTGAACT GTGAATACTT AAATGGTGCA 
CTGGGGGTTC TACGAGAGTC AGAGTGGATC ATTAACACAC AAGTGTGGCC ATGTGAAAGA 
ATGGATGTGC TGAAACGACA TAACCTGTTG TGTACAATTG CACAGAAAAC ACTGAATAAA 
AACATGTTTG ATGTGTGTCT TGAGGTCCTT CAGAATCTGC CAGGACTGCA GTGCTCATTA 
ACGGATGTGG ACGTCTCTCA GTACAGCTTG CTTTTCAACA AACTTTTGTC ATCCTGCATA 
GAAAATAATA CACTTGGTGT ATCCTCAACT GTGATAGATT TTATGATTGC AAAAAAAATA 
CCACTTGACT TTTTCTTACT GAGAGCACTA ATCACATCCT TGGGACGAAG CTGTCTATGG 
CTCAGAGCAA GAGAACTTTA TAAATGCGCT GTTTTGTTGG GTTGCTACCC GCCCATGGAA 
GGAAATCTGT ATCGTAAAGT GCTCTTCATC CCCTCATTCC TTACTGAGAT AGAAATGCTG 
TTATCCATTG AAATTTTCAT GGTGTCAAAT GCCAGTAGTA TTCAGTCACC AGGAGGCAGC 
CATCAGACTC TGCAAATCAT TCTCAAAAGA TGTGAAGAAG AGAGAGTTCC GAATAAAGAT 
TATCAAAGAG GTAAAGCTAG CTATCAAGAT GCAGTGGACC GGCTGATTCA GGCCTCTAGG 
CTATCAACAC CAAGATTATT CATAAAGCAC CTGACTGTCA ATAATGCCAA TGAACAGGTT 
TATATCCTTG ATTACGGCAG CTCTCTCAAA TGGTTGCATG AAAACATGAA ATGGGCTGGG 
AAAGTATGGC TTTTTCAGGG GAAAATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	topaz1
Entrez Gene ID	100380130
Full Name	testis and ovary specific PAZ domain containing 1
Synonyms	c3orf77
General protein information	Preferred Names testis and ovary specific PAZ domain containing 1 Names testis- and ovary-specific PAZ domain-containing protein 1
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

Homo sapiens (human)	TOPAZ1	NP_001138502.1
Mus musculus (house mouse)	Topaz1	NP_001186665.1
Bos taurus (cattle)	TOPAZ1	NP_001239370.1
Rattus norvegicus (Norway rat)	Topaz1	XP_006244266.1
Pan troglodytes (chimpanzee)	TOPAZ1	XP_526186.2
Xenopus tropicalis (tropical clawed frog)	topaz1	NP_001186868.1
	TOPAZ1	XP_001114967.1
Canis lupus familiaris (dog)	TOPAZ1	XP_003433200.2
Gallus gallus (chicken)	TOPAZ1	XP_003640786.2

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

topaz1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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