usp5 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	usp5
Entrez Gene ID	100144735
Full Name	ubiquitin specific peptidase 5
Synonyms	isot,usp5-a,usp5-b
General protein information	Preferred Names ubiquitin specific peptidase 5 Names ubiquitin carboxyl-terminal hydrolase 5 isopeptidase T
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012966040.2	XP_012821494.1	ubiquitin carboxyl-terminal hydrolase 5 isoform X1
XM_012966041.2	XP_012821495.1	ubiquitin carboxyl-terminal hydrolase 5 isoform X2
XM_012966040.3	XP_012821494.1	ubiquitin carboxyl-terminal hydrolase 5 isoform X1
NM_001123484.1	NP_001116956.1	ubiquitin carboxyl-terminal hydrolase 5

Pathways from BioSystems

Homologies

Homo sapiens (human)	USP5	NP_001092006.1
Mus musculus (house mouse)	Usp5	NP_038728.1
Danio rerio (zebrafish)	usp5	NP_999920.2
Drosophila melanogaster (fruit fly)	CG12082	NP_647773.1
Pan troglodytes (chimpanzee)	USP5	XP_508970.4
Bos taurus (cattle)	USP5	NP_001178985.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012248	XP_320294.3
	USP5	XP_001110639.1
Canis lupus familiaris (dog)	USP5	XP_543845.3
Rattus norvegicus (Norway rat)	Usp5	NP_001100089.1
Gallus gallus (chicken)	USP5	XP_003640490.1
Oryza sativa (rice)	Os01g0177200	NP_001042185.1
Arabidopsis thaliana (thale cress)	UBP14	NP_566666.2
Xenopus tropicalis (tropical clawed frog)	usp5	NP_001116956.1

Gene Ontology
Bibliography

Related articles in PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Klein SL, Strausberg RL, Wagner L, Pontius J, Clifton SW, Richardson P
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following usp5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the usp5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa22321	NM_001123484.1 Latest version!	Xenopus tropicalis ubiquitin specific peptidase 5 (usp5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OXa31426	XM_012966041.2 Latest version!	Xenopus tropicalis ubiquitin specific peptidase 5 (isopeptidase T) (usp5), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OXa31425	XM_012966040.3 Latest version!	Xenopus tropicalis ubiquitin specific peptidase 5 (usp5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
View Old Accession Versions >>
OXa31425	XM_012966040.2	Xenopus tropicalis ubiquitin specific peptidase 5 (isopeptidase T) (usp5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

usp5 ( NM_001123484.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> NP_001116956.1 Xenopus tropicalis ubiquitin specific peptidase 5 (usp5), mRNA.

Price & Availability↑

CloneID	OXa22321
Clone ID Related Accession (Same CDS sequence)	NM_001123484.1
Accession Version	NM_001123484.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2499bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-21
Organism	Xenopus tropicalis(tropical clawed frog)
Product	ubiquitin carboxyl-terminal hydrolase 5
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC161109.1. ##Evidence-Data-START## Transcript exon combination :: BC161109.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00861997 [ECO:0000348] ##Evidence-Data-END##

ATGGGTGATG TGAAAGATGC TCTGCTCTCT GTGCTACCGA CCATTCGGCT ACCTCGGGCT 
GGGGACCGAG TGCACAAGGA TGAGTGTGCA TATACCTTCC ATACTCCGGA GACAGACAGC 
GGATTATATA TTTGTATGAA TACATTTCTG GGCTTTGGGA GGCCCTATGT TGAGCGGCAA 
TACAACAAGA CTGGGCAGAG AGCCTTTCTG CATCTTAAGA GGACTGCTAA AATGAAATCT 
GAGGAATCTA ATTCCGGCAC AGGAGACCCC CCACGCAAGA AGCCGACACG CTTAGCCATT 
GGAATGGAGG GCGGCTTTGA CATGAGTGAG GAACAGTGTG AATATGAGGA GGTGGTGAAG 
TTGGTCATTC TGCCTGAGTT CCTAGAGATT CCACGGGATG GACTGTTGGG GCTCCCGGAG 
ATGGTCAGGG ATCGGGTGTC GTCTGCTATT GATGCCATAC TGTCTGCTGA CTCCGCCTCC 
CGGAAACAGG AAATTCAGGC CTGGGACGGG GAAGTGAGGC AGGTTTCCAA ACATGCGTTC 
AACCTCCAGC AGCTTGAGGG GGTACCCCGT ATCCCACCTT GTGGATGGAA GTGCAGTGTC 
TGTGATCTGC GGGAGAATCT GTGGCTCAAT ATGACAGACG GCGCCATCCT CTGCGGAAGA 
CGTTACTTTG ATGGCAGCGG AGGGAATAAC CACGCGTTAC AGCACTACCA AGAAACAGGC 
TACCCGTTAG CAGTGAAACT GGGTACCATT ACGCCAGATG GAGGCGATGT ATATTCCTAT 
GATGAGGATG ATATGGTTCT GGACCCCAAC CTGGCAGAGC ATCTCTCACA CTTTGGCATT 
GACATGATGA AGATGCAGAA GACGGATAAG ACCATGACAG AACTGGAAAT TGATATGAAC 
CAACGAATCG GAGAGTGGGA GGTGATCCAG GAATCAGGTG TACAATTGCA GCCCCTCTAT 
GGTTCGGGGT ACACTGGCAT CCAGAACCTC GGAAACAGCT GCTACCTCAA TTCAACCATG 
CAAGTCATCT TCAGCATCCC GGCCTTCCAG CGCAAATACG TAGAACGTAT GGAGCAGATT 
TTCCAGAAAT CACCTGCTGA TCCAACGCAA GACTTCAACA CCCAAGTAGC CAAGCTTGGA 
AATGGCATGT TATCTGGGGA ATACTCAAAG CCAGCTGTAG AAAAAGACAA TGACACAGCT 
CCAGAACATA AGGGTCTTCA GGATGGGATT GCTCCGAGAA TGTTTAAGGC ACTTGTTGGG 
AAGGGACACC CAGAGTTTTC CACCAACAGG CAGCAAGATG CTCAGGAATT CTTCCTGCAC 
TTTATAAATA TGGTTGAGAG GAACTGCCGC AGCTCATCCA ACCCCAATGA AGTTTTCCGT 
TTCCTAGTGG AAGAGAAGAT TAAGTGTCTG GTGTCTCAGA AAGTGAAGTA TACACAGAGG 
GTTGAGTACA TCCTGCAACT TCCTGTGCCT ATGGAGGCTG CACTCAATAA GGACGAGCTG 
TCTGCATATG AGCAATGCCG GCTCCAGGCT GAACGTGAGC ATCGCCCCCC GCCAGAACTA 
GTCCGGGCAC GGATCCCATT CACATCATGT TTGGAGGCAT TTGCTGCCCC TGAGCAGCTC 
GATGAATTCT GGAGCGCCGC CCTTCAGGCC AAATCCAATG CACTTAAGAC GTCCCGCTTT 
GCCTCATTTC CAGATTACTT GGTCATTCAA ATCAAGAAGT TTACTTTTGG CCTTGACTGG 
GTACCCAAGA AACTGGATGT CTCTATTGAT GTCCCAGATG AATTGGATCT TTCTGAGTTT 
CGGGGTGGGG GGCTTCAAGG GGGGGAAGAG GAGCTCCCAG ATGTTGCCCC CCCATTGGTC 
ACACCCGATG AGCCCAAAGC TCCAGTGCTG GACGAGTCAG TAGTCACGCA GTTGGTAGAG 
ATGGGATTCC CAGCCGAGGC GTGCCGCAAA GCTGTGTATT ACACGGGCAA CAGCGGGGCA 
GAGGCTGCAA TGACGTGGGT GATGTCGCAC ATGGATGACC CAGACTTTGC CTTGCCACTG 
TCTGGGGCCA GCGCACCCTC ATCAGTTACT ACCGGGGGGG ATCCACCCTC TGAGGAACAC 
GTAGCAACAA TAATATCCAT GGGCTTCTCC CAGGAGCAAG CAGTGAAAGC CCTCCGTGCC 
ACTAACAACA ACTTGGAACG TGCTGTCGAC TGGATCTTCT CTCACATTGA TGATTTGGAT 
GCTGAGGCTG CCATGGATTT ATCTGAGGGT CGCTCTGCTG CAGAGTCTGT GTCTGAGAGT 
CTCCCTGCTG CGGGCCCTCG TGTGAGGGAT GGAGCTGGGA GATATGAGCT TTTTGCCTTC 
ATCAGTCACA TGGGGACATC CACCATGTGC GGTCACTACG TGTGTCATAT AAAGAAGGAG 
GGAAGGTGGG TGATCTACAA TGACCAGAAA GTCTGCGCTT CAGAGAAGCC TCCCAAAGAC 
CTGGGTTACA TTTACTTCTA CCAGCGTATA CAGAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001116956.1
CDS	34..2532
Translation	MGDVKDALLSVLPTIRLPRAGDRVHKDECAYTFHTPETDSGLYICMNTFLGFGRPYVERQYNKTGQRAFLHLKRTAKMKSEESNSGTGDPPRKKPTRLAIGMEGGFDMSEEQCEYEEVVKLVILPEFLEIPRDGLLGLPEMVRDRVSSAIDAILSADSASRKQEIQAWDGEVRQVSKHAFNLQQLEGVPRIPPCGWKCSVCDLRENLWLNMTDGAILCGRRYFDGSGGNNHALQHYQETGYPLAVKLGTITPDGGDVYSYDEDDMVLDPNLAEHLSHFGIDMMKMQKTDKTMTELEIDMNQRIGEWEVIQESGVQLQPLYGSGYTGIQNLGNSCYLNSTMQVIFSIPAFQRKYVERMEQIFQKSPADPTQDFNTQVAKLGNGMLSGEYSKPAVEKDNDTAPEHKGLQDGIAPRMFKALVGKGHPEFSTNRQQDAQEFFLHFINMVERNCRSSSNPNEVFRFLVEEKIKCLVSQKVKYTQRVEYILQLPVPMEAALNKDELSAYEQCRLQAEREHRPPPELVRARIPFTSCLEAFAAPEQLDEFWSAALQAKSNALKTSRFASFPDYLVIQIKKFTFGLDWVPKKLDVSIDVPDELDLSEFRGGGLQGGEEELPDVAPPLVTPDEPKAPVLDESVVTQLVEMGFPAEACRKAVYYTGNSGAEAAMTWVMSHMDDPDFALPLSGASAPSSVTTGGDPPSEEHVATIISMGFSQEQAVKALRATNNNLERAVDWIFSHIDDLDAEAAMDLSEGRSAAESVSESLPAAGPRVRDGAGRYELFAFISHMGTSTMCGHYVCHIKKEGRWVIYNDQKVCASEKPPKDLGYIYFYQRIQS

Target ORF information:

RefSeq Version	NM_001123484.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ubiquitin specific peptidase 5 (usp5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001123484.1

ORF Insert Sequence:

ATGGGTGATG TGAAAGATGC TCTGCTCTCT GTGCTACCGA CCATTCGGCT ACCTCGGGCT 
GGGGACCGAG TGCACAAGGA TGAGTGTGCA TATACCTTCC ATACTCCGGA GACAGACAGC 
GGATTATATA TTTGTATGAA TACATTTCTG GGCTTTGGGA GGCCCTATGT TGAGCGGCAA 
TACAACAAGA CTGGGCAGAG AGCCTTTCTG CATCTTAAGA GGACTGCTAA AATGAAATCT 
GAGGAATCTA ATTCCGGCAC AGGAGACCCC CCACGCAAGA AGCCGACACG CTTAGCCATT 
GGAATGGAGG GCGGCTTTGA CATGAGTGAG GAACAGTGTG AATATGAGGA GGTGGTGAAG 
TTGGTCATTC TGCCTGAGTT CCTAGAGATT CCACGGGATG GACTGTTGGG GCTCCCGGAG 
ATGGTCAGGG ATCGGGTGTC GTCTGCTATT GATGCCATAC TGTCTGCTGA CTCCGCCTCC 
CGGAAACAGG AAATTCAGGC CTGGGACGGG GAAGTGAGGC AGGTTTCCAA ACATGCGTTC 
AACCTCCAGC AGCTTGAGGG GGTACCCCGT ATCCCACCTT GTGGATGGAA GTGCAGTGTC 
TGTGATCTGC GGGAGAATCT GTGGCTCAAT ATGACAGACG GCGCCATCCT CTGCGGAAGA 
CGTTACTTTG ATGGCAGCGG AGGGAATAAC CACGCGTTAC AGCACTACCA AGAAACAGGC 
TACCCGTTAG CAGTGAAACT GGGTACCATT ACGCCAGATG GAGGCGATGT ATATTCCTAT 
GATGAGGATG ATATGGTTCT GGACCCCAAC CTGGCAGAGC ATCTCTCACA CTTTGGCATT 
GACATGATGA AGATGCAGAA GACGGATAAG ACCATGACAG AACTGGAAAT TGATATGAAC 
CAACGAATCG GAGAGTGGGA GGTGATCCAG GAATCAGGTG TACAATTGCA GCCCCTCTAT 
GGTTCGGGGT ACACTGGCAT CCAGAACCTC GGAAACAGCT GCTACCTCAA TTCAACCATG 
CAAGTCATCT TCAGCATCCC GGCCTTCCAG CGCAAATACG TAGAACGTAT GGAGCAGATT 
TTCCAGAAAT CACCTGCTGA TCCAACGCAA GACTTCAACA CCCAAGTAGC CAAGCTTGGA 
AATGGCATGT TATCTGGGGA ATACTCAAAG CCAGCTGTAG AAAAAGACAA TGACACAGCT 
CCAGAACATA AGGGTCTTCA GGATGGGATT GCTCCGAGAA TGTTTAAGGC ACTTGTTGGG 
AAGGGACACC CAGAGTTTTC CACCAACAGG CAGCAAGATG CTCAGGAATT CTTCCTGCAC 
TTTATAAATA TGGTTGAGAG GAACTGCCGC AGCTCATCCA ACCCCAATGA AGTTTTCCGT 
TTCCTAGTGG AAGAGAAGAT TAAGTGTCTG GTGTCTCAGA AAGTGAAGTA TACACAGAGG 
GTTGAGTACA TCCTGCAACT TCCTGTGCCT ATGGAGGCTG CACTCAATAA GGACGAGCTG 
TCTGCATATG AGCAATGCCG GCTCCAGGCT GAACGTGAGC ATCGCCCCCC GCCAGAACTA 
GTCCGGGCAC GGATCCCATT CACATCATGT TTGGAGGCAT TTGCTGCCCC TGAGCAGCTC 
GATGAATTCT GGAGCGCCGC CCTTCAGGCC AAATCCAATG CACTTAAGAC GTCCCGCTTT 
GCCTCATTTC CAGATTACTT GGTCATTCAA ATCAAGAAGT TTACTTTTGG CCTTGACTGG 
GTACCCAAGA AACTGGATGT CTCTATTGAT GTCCCAGATG AATTGGATCT TTCTGAGTTT 
CGGGGTGGGG GGCTTCAAGG GGGGGAAGAG GAGCTCCCAG ATGTTGCCCC CCCATTGGTC 
ACACCCGATG AGCCCAAAGC TCCAGTGCTG GACGAGTCAG TAGTCACGCA GTTGGTAGAG 
ATGGGATTCC CAGCCGAGGC GTGCCGCAAA GCTGTGTATT ACACGGGCAA CAGCGGGGCA 
GAGGCTGCAA TGACGTGGGT GATGTCGCAC ATGGATGACC CAGACTTTGC CTTGCCACTG 
TCTGGGGCCA GCGCACCCTC ATCAGTTACT ACCGGGGGGG ATCCACCCTC TGAGGAACAC 
GTAGCAACAA TAATATCCAT GGGCTTCTCC CAGGAGCAAG CAGTGAAAGC CCTCCGTGCC 
ACTAACAACA ACTTGGAACG TGCTGTCGAC TGGATCTTCT CTCACATTGA TGATTTGGAT 
GCTGAGGCTG CCATGGATTT ATCTGAGGGT CGCTCTGCTG CAGAGTCTGT GTCTGAGAGT 
CTCCCTGCTG CGGGCCCTCG TGTGAGGGAT GGAGCTGGGA GATATGAGCT TTTTGCCTTC 
ATCAGTCACA TGGGGACATC CACCATGTGC GGTCACTACG TGTGTCATAT AAAGAAGGAG 
GGAAGGTGGG TGATCTACAA TGACCAGAAA GTCTGCGCTT CAGAGAAGCC TCCCAAAGAC 
CTGGGTTACA TTTACTTCTA CCAGCGTATA CAGAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

usp5 ( XM_012966040.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012821494.1 Xenopus tropicalis ubiquitin specific peptidase 5 (isopeptidase T) (usp5), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa31425
Clone ID Related Accession (Same CDS sequence)	XM_012966040.2 , XM_012966040.3
Accession Version	XM_012966040.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2568bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	ubiquitin carboxyl-terminal hydrolase 5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012966040.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTGATG TGAAAGATGC TCTGCTCTCT GTGCTACCGA CCATTCGGCT ACCTCGGGCT 
GGGGACCGAG TGCACAAGGA TGAGTGTGCA TATACCTTCC ATACTCCGGA GACAGACAGC 
GGATTATATA TTTGTATGAA TACATTTTTG GGCTTTGGGA GGCCCTATGT TGAGCGGCAA 
TACAACAAGA CTGGGCAGAG AGCCTTTCTG CATCTTAAGA GGACTGCTAA AATGAAATCT 
GAGGAATCTA ATTCCGGCAC AGGAGACCCC CCACGCAAGA AGCCGACACG CTTAGCCATT 
GGAATGGAGG GCGGCTTTGA CATGAGTGAG GAACAGTGTG AATATGAGGA GGTGGTGAAG 
TTGGTCATTC TGCCTGAGTT CCTAGAGATT CCACGGGATG GACTGTTGGG GCTCCCGGAG 
ATGGTCAGGG ATCGGGTGTC GTCTGCTATT GATGCCATAC TGTCTGCTGA CTCCGCCTCC 
CGGAAACAGG AAATTCAGGC CTGGGACGGG GAAGTGAGGC AGGTTTCCAA ACATGCGTTC 
AACCTCCAGC AGCTTGAGGG GGTACCCCGT ATCCCACCTT GTGGATGGAA GTGCAGTGTC 
TGTGATCTGC GGGAGAATCT GTGGCTCAAT ATGACAGACG GCGCCATCCT CTGCGGAAGA 
CGTTACTTTG ATGGCAGCGG AGGGAATAAC CACGCGTTAC AGCACTACCA AGAAACAGGC 
TACCCGTTAG CAGTGAAACT GGGCACCATT ACGCCAGATG GAGGCGATGT ATATTCCTAT 
GATGAGGATG ATATGGTTCT GGACCCCAAC CTGGCAGAGC ATCTCTCACA CTTTGGCATT 
GACATGATGA AGATGCAGAA GACGGATAAG ACCATGACAG AACTGGAAAT TGATATGAAC 
CAACGAATCG GAGAGTGGGA GGTGATCCAG GAATCAGGTG TACAATTGCA GCCCCTCTAT 
GGTTCGGGGT ACACTGGCAT CCAGAACCTC GGAAACAGCT GCTACCTCAA TTCAACCATG 
CAAGTCATCT TCAGCATCCC GGCCTTCCAG CGCAAATACG TAGAACGTAT GGAGCAGATT 
TTCCAGAAAT CACCTGCTGA TCCAACGCAA GACTTCAACA CCCAAGTAGC CAAGCTTGGA 
AATGGCATGT TATCTGGGGA ATACTCAAAG CCAGCTGTAG AAAAAGACAA TGACACAGCT 
CCAGAACATA AGGGTCTTCA GGATGGGATT GCTCCGAGAA TGTTTAAGGC ACTTGTTGGG 
AAGGGACACC CAGAGTTTTC CACCAACAGG CAGCAAGATG CTCAGGAATT CTTCCTGCAC 
TTTATAAATA TGGTTGAGAG GAACTGCCGC AGCTCATCCA ACCCCAATGA AGTTTTCCGT 
TTCCTAGTGG AAGAGAAGAT TAAGTGTCTG GTGTCTCAGA AAGTGAAGTA TACACAGAGG 
GTTGAGTACA TCCTGCAACT TCCTGTGCCC ATGGAGGCTG CACTCAATAA GGACGAGCTG 
TCTGCATATG AGCAATGCCG GCTCCAGGCT GAACGTGAGC ATCGCCCCCC GCCAGAACTA 
GTCCGGGCAC GGATCCCATT CACATCATGT TTGGAGGCAT TTGCTGCCCC TGAGCAGCTC 
GATGAATTCT GGAGCGCCGC CCTTCAGGCC AAATCCAATG CACTTAAGAC GTCCCGCTTT 
GCCTCATTTC CAGATTACTT GGTCATTCAA ATCAAGAAGT TTACTTTTGG CCTTGACTGG 
GTACCCAAGA AACTGGATGT CTCTATTGAT GTCCCAGATG AATTGGATCT TTCTGAGTTT 
CGGGGTGGGG GGCTTCAAGG GGGGGAAGAG GAGCTCCCAG ATGTTGCCCC CCCATTGGTC 
ACACCCGATG AGCCCAAAGG TAGCCTTGGT TTCTATGGCC TCGACGATGA CGACTCGTAC 
TGCTCCCCTC ACTTCTCCTC TCCGACATCT CCAGTGCTGG ACGAGTCAGT AGTCACGCAG 
TTGGTAGAGA TGGGATTCCC AGCCGAGGCG TGCCGCAAAG CTGTGTATTA TACGGGCAAC 
AGCGGGGCAG AGGCTGCAAT GACGTGGGTG ATGTCGCACA TGGATGACCC AGACTTTGCC 
TTGCCACTGT CTGGGGCCAG CGCACCCTCA TCAGTTACTA CCGGGGGGGA TCCACCCTCT 
GAGGAACACG TAGCAACAAT AATATCCATG GGCTTCTCCC AGGAGCAAGC AGTGAAAGCC 
CTCCGTGCCA CTAACAACAA CTTGGAACGT GCTGTCGACT GGATCTTCTC TCACATTGAT 
GATTTGGATG CTGAGGCTGC CATGGATTTA TCTGAGGGTC GCTCTGCTGC AGAGTCTGTG 
TCTGAGAGTC TCCCTGCTGC GGGCCCTCGT GTGAGGGATG GAGCTGGGAG ATATGAGCTT 
TTTGCCTTCA TCAGTCACAT GGGGACATCC ACCATGTGCG GTCACTACGT GTGTCATATA 
AAGAAGGAGG GAAGGTGGGT GATCTACAAT GACCAGAAAG TCTGCGCTTC AGAGAAGCCT 
CCCAAAGACC TGGGTTACAT TTACTTCTAC CAGCGTATAC AGAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012821494.1
CDS	122..2689
Translation	MGDVKDALLSVLPTIRLPRAGDRVHKDECAYTFHTPETDSGLYICMNTFLGFGRPYVERQYNKTGQRAFLHLKRTAKMKSEESNSGTGDPPRKKPTRLAIGMEGGFDMSEEQCEYEEVVKLVILPEFLEIPRDGLLGLPEMVRDRVSSAIDAILSADSASRKQEIQAWDGEVRQVSKHAFNLQQLEGVPRIPPCGWKCSVCDLRENLWLNMTDGAILCGRRYFDGSGGNNHALQHYQETGYPLAVKLGTITPDGGDVYSYDEDDMVLDPNLAEHLSHFGIDMMKMQKTDKTMTELEIDMNQRIGEWEVIQESGVQLQPLYGSGYTGIQNLGNSCYLNSTMQVIFSIPAFQRKYVERMEQIFQKSPADPTQDFNTQVAKLGNGMLSGEYSKPAVEKDNDTAPEHKGLQDGIAPRMFKALVGKGHPEFSTNRQQDAQEFFLHFINMVERNCRSSSNPNEVFRFLVEEKIKCLVSQKVKYTQRVEYILQLPVPMEAALNKDELSAYEQCRLQAEREHRPPPELVRARIPFTSCLEAFAAPEQLDEFWSAALQAKSNALKTSRFASFPDYLVIQIKKFTFGLDWVPKKLDVSIDVPDELDLSEFRGGGLQGGEEELPDVAPPLVTPDEPKGSLGFYGLDDDDSYCSPHFSSPTSPVLDESVVTQLVEMGFPAEACRKAVYYTGNSGAEAAMTWVMSHMDDPDFALPLSGASAPSSVTTGGDPPSEEHVATIISMGFSQEQAVKALRATNNNLERAVDWIFSHIDDLDAEAAMDLSEGRSAAESVSESLPAAGPRVRDGAGRYELFAFISHMGTSTMCGHYVCHIKKEGRWVIYNDQKVCASEKPPKDLGYIYFYQRIQS

Target ORF information:

RefSeq Version	XM_012966040.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ubiquitin specific peptidase 5 (isopeptidase T) (usp5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012966040.2

ORF Insert Sequence:

ATGGGTGATG TGAAAGATGC TCTGCTCTCT GTGCTACCGA CCATTCGGCT ACCTCGGGCT 
GGGGACCGAG TGCACAAGGA TGAGTGTGCA TATACCTTCC ATACTCCGGA GACAGACAGC 
GGATTATATA TTTGTATGAA TACATTTTTG GGCTTTGGGA GGCCCTATGT TGAGCGGCAA 
TACAACAAGA CTGGGCAGAG AGCCTTTCTG CATCTTAAGA GGACTGCTAA AATGAAATCT 
GAGGAATCTA ATTCCGGCAC AGGAGACCCC CCACGCAAGA AGCCGACACG CTTAGCCATT 
GGAATGGAGG GCGGCTTTGA CATGAGTGAG GAACAGTGTG AATATGAGGA GGTGGTGAAG 
TTGGTCATTC TGCCTGAGTT CCTAGAGATT CCACGGGATG GACTGTTGGG GCTCCCGGAG 
ATGGTCAGGG ATCGGGTGTC GTCTGCTATT GATGCCATAC TGTCTGCTGA CTCCGCCTCC 
CGGAAACAGG AAATTCAGGC CTGGGACGGG GAAGTGAGGC AGGTTTCCAA ACATGCGTTC 
AACCTCCAGC AGCTTGAGGG GGTACCCCGT ATCCCACCTT GTGGATGGAA GTGCAGTGTC 
TGTGATCTGC GGGAGAATCT GTGGCTCAAT ATGACAGACG GCGCCATCCT CTGCGGAAGA 
CGTTACTTTG ATGGCAGCGG AGGGAATAAC CACGCGTTAC AGCACTACCA AGAAACAGGC 
TACCCGTTAG CAGTGAAACT GGGCACCATT ACGCCAGATG GAGGCGATGT ATATTCCTAT 
GATGAGGATG ATATGGTTCT GGACCCCAAC CTGGCAGAGC ATCTCTCACA CTTTGGCATT 
GACATGATGA AGATGCAGAA GACGGATAAG ACCATGACAG AACTGGAAAT TGATATGAAC 
CAACGAATCG GAGAGTGGGA GGTGATCCAG GAATCAGGTG TACAATTGCA GCCCCTCTAT 
GGTTCGGGGT ACACTGGCAT CCAGAACCTC GGAAACAGCT GCTACCTCAA TTCAACCATG 
CAAGTCATCT TCAGCATCCC GGCCTTCCAG CGCAAATACG TAGAACGTAT GGAGCAGATT 
TTCCAGAAAT CACCTGCTGA TCCAACGCAA GACTTCAACA CCCAAGTAGC CAAGCTTGGA 
AATGGCATGT TATCTGGGGA ATACTCAAAG CCAGCTGTAG AAAAAGACAA TGACACAGCT 
CCAGAACATA AGGGTCTTCA GGATGGGATT GCTCCGAGAA TGTTTAAGGC ACTTGTTGGG 
AAGGGACACC CAGAGTTTTC CACCAACAGG CAGCAAGATG CTCAGGAATT CTTCCTGCAC 
TTTATAAATA TGGTTGAGAG GAACTGCCGC AGCTCATCCA ACCCCAATGA AGTTTTCCGT 
TTCCTAGTGG AAGAGAAGAT TAAGTGTCTG GTGTCTCAGA AAGTGAAGTA TACACAGAGG 
GTTGAGTACA TCCTGCAACT TCCTGTGCCC ATGGAGGCTG CACTCAATAA GGACGAGCTG 
TCTGCATATG AGCAATGCCG GCTCCAGGCT GAACGTGAGC ATCGCCCCCC GCCAGAACTA 
GTCCGGGCAC GGATCCCATT CACATCATGT TTGGAGGCAT TTGCTGCCCC TGAGCAGCTC 
GATGAATTCT GGAGCGCCGC CCTTCAGGCC AAATCCAATG CACTTAAGAC GTCCCGCTTT 
GCCTCATTTC CAGATTACTT GGTCATTCAA ATCAAGAAGT TTACTTTTGG CCTTGACTGG 
GTACCCAAGA AACTGGATGT CTCTATTGAT GTCCCAGATG AATTGGATCT TTCTGAGTTT 
CGGGGTGGGG GGCTTCAAGG GGGGGAAGAG GAGCTCCCAG ATGTTGCCCC CCCATTGGTC 
ACACCCGATG AGCCCAAAGG TAGCCTTGGT TTCTATGGCC TCGACGATGA CGACTCGTAC 
TGCTCCCCTC ACTTCTCCTC TCCGACATCT CCAGTGCTGG ACGAGTCAGT AGTCACGCAG 
TTGGTAGAGA TGGGATTCCC AGCCGAGGCG TGCCGCAAAG CTGTGTATTA TACGGGCAAC 
AGCGGGGCAG AGGCTGCAAT GACGTGGGTG ATGTCGCACA TGGATGACCC AGACTTTGCC 
TTGCCACTGT CTGGGGCCAG CGCACCCTCA TCAGTTACTA CCGGGGGGGA TCCACCCTCT 
GAGGAACACG TAGCAACAAT AATATCCATG GGCTTCTCCC AGGAGCAAGC AGTGAAAGCC 
CTCCGTGCCA CTAACAACAA CTTGGAACGT GCTGTCGACT GGATCTTCTC TCACATTGAT 
GATTTGGATG CTGAGGCTGC CATGGATTTA TCTGAGGGTC GCTCTGCTGC AGAGTCTGTG 
TCTGAGAGTC TCCCTGCTGC GGGCCCTCGT GTGAGGGATG GAGCTGGGAG ATATGAGCTT 
TTTGCCTTCA TCAGTCACAT GGGGACATCC ACCATGTGCG GTCACTACGT GTGTCATATA 
AAGAAGGAGG GAAGGTGGGT GATCTACAAT GACCAGAAAG TCTGCGCTTC AGAGAAGCCT 
CCCAAAGACC TGGGTTACAT TTACTTCTAC CAGCGTATAC AGAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

usp5 ( XM_012966041.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012821495.1 Xenopus tropicalis ubiquitin specific peptidase 5 (isopeptidase T) (usp5), transcript variant X2, mRNA.

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CloneID	OXa31426
Clone ID Related Accession (Same CDS sequence)	XM_012966041.2
Accession Version	XM_012966041.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2433bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	ubiquitin carboxyl-terminal hydrolase 5 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012966041.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATACAT TTTTGGGCTT TGGGAGGCCC TATGTTGAGC GGCAATACAA CAAGACTGGG 
CAGAGAGCCT TTCTGCATCT TAAGAGGACT GCTAAAATGA AATCTGAGGA ATCTAATTCC 
GGCACAGGAG ACCCCCCACG CAAGAAGCCG ACACGCTTAG CCATTGGAAT GGAGGGCGGC 
TTTGACATGA GTGAGGAACA GTGTGAATAT GAGGAGGTGG TGAAGTTGGT CATTCTGCCT 
GAGTTCCTAG AGATTCCACG GGATGGACTG TTGGGGCTCC CGGAGATGGT CAGGGATCGG 
GTGTCGTCTG CTATTGATGC CATACTGTCT GCTGACTCCG CCTCCCGGAA ACAGGAAATT 
CAGGCCTGGG ACGGGGAAGT GAGGCAGGTT TCCAAACATG CGTTCAACCT CCAGCAGCTT 
GAGGGGGTAC CCCGTATCCC ACCTTGTGGA TGGAAGTGCA GTGTCTGTGA TCTGCGGGAG 
AATCTGTGGC TCAATATGAC AGACGGCGCC ATCCTCTGCG GAAGACGTTA CTTTGATGGC 
AGCGGAGGGA ATAACCACGC GTTACAGCAC TACCAAGAAA CAGGCTACCC GTTAGCAGTG 
AAACTGGGCA CCATTACGCC AGATGGAGGC GATGTATATT CCTATGATGA GGATGATATG 
GTTCTGGACC CCAACCTGGC AGAGCATCTC TCACACTTTG GCATTGACAT GATGAAGATG 
CAGAAGACGG ATAAGACCAT GACAGAACTG GAAATTGATA TGAACCAACG AATCGGAGAG 
TGGGAGGTGA TCCAGGAATC AGGTGTACAA TTGCAGCCCC TCTATGGTTC GGGGTACACT 
GGCATCCAGA ACCTCGGAAA CAGCTGCTAC CTCAATTCAA CCATGCAAGT CATCTTCAGC 
ATCCCGGCCT TCCAGCGCAA ATACGTAGAA CGTATGGAGC AGATTTTCCA GAAATCACCT 
GCTGATCCAA CGCAAGACTT CAACACCCAA GTAGCCAAGC TTGGAAATGG CATGTTATCT 
GGGGAATACT CAAAGCCAGC TGTAGAAAAA GACAATGACA CAGCTCCAGA ACATAAGGGT 
CTTCAGGATG GGATTGCTCC GAGAATGTTT AAGGCACTTG TTGGGAAGGG ACACCCAGAG 
TTTTCCACCA ACAGGCAGCA AGATGCTCAG GAATTCTTCC TGCACTTTAT AAATATGGTT 
GAGAGGAACT GCCGCAGCTC ATCCAACCCC AATGAAGTTT TCCGTTTCCT AGTGGAAGAG 
AAGATTAAGT GTCTGGTGTC TCAGAAAGTG AAGTATACAC AGAGGGTTGA GTACATCCTG 
CAACTTCCTG TGCCCATGGA GGCTGCACTC AATAAGGACG AGCTGTCTGC ATATGAGCAA 
TGCCGGCTCC AGGCTGAACG TGAGCATCGC CCCCCGCCAG AACTAGTCCG GGCACGGATC 
CCATTCACAT CATGTTTGGA GGCATTTGCT GCCCCTGAGC AGCTCGATGA ATTCTGGAGC 
GCCGCCCTTC AGGCCAAATC CAATGCACTT AAGACGTCCC GCTTTGCCTC ATTTCCAGAT 
TACTTGGTCA TTCAAATCAA GAAGTTTACT TTTGGCCTTG ACTGGGTACC CAAGAAACTG 
GATGTCTCTA TTGATGTCCC AGATGAATTG GATCTTTCTG AGTTTCGGGG TGGGGGGCTT 
CAAGGGGGGG AAGAGGAGCT CCCAGATGTT GCCCCCCCAT TGGTCACACC CGATGAGCCC 
AAAGGTAGCC TTGGTTTCTA TGGCCTCGAC GATGACGACT CGTACTGCTC CCCTCACTTC 
TCCTCTCCGA CATCTCCAGT GCTGGACGAG TCAGTAGTCA CGCAGTTGGT AGAGATGGGA 
TTCCCAGCCG AGGCGTGCCG CAAAGCTGTG TATTATACGG GCAACAGCGG GGCAGAGGCT 
GCAATGACGT GGGTGATGTC GCACATGGAT GACCCAGACT TTGCCTTGCC ACTGTCTGGG 
GCCAGCGCAC CCTCATCAGT TACTACCGGG GGGGATCCAC CCTCTGAGGA ACACGTAGCA 
ACAATAATAT CCATGGGCTT CTCCCAGGAG CAAGCAGTGA AAGCCCTCCG TGCCACTAAC 
AACAACTTGG AACGTGCTGT CGACTGGATC TTCTCTCACA TTGATGATTT GGATGCTGAG 
GCTGCCATGG ATTTATCTGA GGGTCGCTCT GCTGCAGAGT CTGTGTCTGA GAGTCTCCCT 
GCTGCGGGCC CTCGTGTGAG GGATGGAGCT GGGAGATATG AGCTTTTTGC CTTCATCAGT 
CACATGGGGA CATCCACCAT GTGCGGTCAC TACGTGTGTC ATATAAAGAA GGAGGGAAGG 
TGGGTGATCT ACAATGACCA GAAAGTCTGC GCTTCAGAGA AGCCTCCCAA AGACCTGGGT 
TACATTTACT TCTACCAGCG TATACAGAGT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012821495.1
CDS	599..3031
Translation	MNTFLGFGRPYVERQYNKTGQRAFLHLKRTAKMKSEESNSGTGDPPRKKPTRLAIGMEGGFDMSEEQCEYEEVVKLVILPEFLEIPRDGLLGLPEMVRDRVSSAIDAILSADSASRKQEIQAWDGEVRQVSKHAFNLQQLEGVPRIPPCGWKCSVCDLRENLWLNMTDGAILCGRRYFDGSGGNNHALQHYQETGYPLAVKLGTITPDGGDVYSYDEDDMVLDPNLAEHLSHFGIDMMKMQKTDKTMTELEIDMNQRIGEWEVIQESGVQLQPLYGSGYTGIQNLGNSCYLNSTMQVIFSIPAFQRKYVERMEQIFQKSPADPTQDFNTQVAKLGNGMLSGEYSKPAVEKDNDTAPEHKGLQDGIAPRMFKALVGKGHPEFSTNRQQDAQEFFLHFINMVERNCRSSSNPNEVFRFLVEEKIKCLVSQKVKYTQRVEYILQLPVPMEAALNKDELSAYEQCRLQAEREHRPPPELVRARIPFTSCLEAFAAPEQLDEFWSAALQAKSNALKTSRFASFPDYLVIQIKKFTFGLDWVPKKLDVSIDVPDELDLSEFRGGGLQGGEEELPDVAPPLVTPDEPKGSLGFYGLDDDDSYCSPHFSSPTSPVLDESVVTQLVEMGFPAEACRKAVYYTGNSGAEAAMTWVMSHMDDPDFALPLSGASAPSSVTTGGDPPSEEHVATIISMGFSQEQAVKALRATNNNLERAVDWIFSHIDDLDAEAAMDLSEGRSAAESVSESLPAAGPRVRDGAGRYELFAFISHMGTSTMCGHYVCHIKKEGRWVIYNDQKVCASEKPPKDLGYIYFYQRIQS

Target ORF information:

RefSeq Version	XM_012966041.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ubiquitin specific peptidase 5 (isopeptidase T) (usp5), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012966041.2

ORF Insert Sequence:

ATGAATACAT TTTTGGGCTT TGGGAGGCCC TATGTTGAGC GGCAATACAA CAAGACTGGG 
CAGAGAGCCT TTCTGCATCT TAAGAGGACT GCTAAAATGA AATCTGAGGA ATCTAATTCC 
GGCACAGGAG ACCCCCCACG CAAGAAGCCG ACACGCTTAG CCATTGGAAT GGAGGGCGGC 
TTTGACATGA GTGAGGAACA GTGTGAATAT GAGGAGGTGG TGAAGTTGGT CATTCTGCCT 
GAGTTCCTAG AGATTCCACG GGATGGACTG TTGGGGCTCC CGGAGATGGT CAGGGATCGG 
GTGTCGTCTG CTATTGATGC CATACTGTCT GCTGACTCCG CCTCCCGGAA ACAGGAAATT 
CAGGCCTGGG ACGGGGAAGT GAGGCAGGTT TCCAAACATG CGTTCAACCT CCAGCAGCTT 
GAGGGGGTAC CCCGTATCCC ACCTTGTGGA TGGAAGTGCA GTGTCTGTGA TCTGCGGGAG 
AATCTGTGGC TCAATATGAC AGACGGCGCC ATCCTCTGCG GAAGACGTTA CTTTGATGGC 
AGCGGAGGGA ATAACCACGC GTTACAGCAC TACCAAGAAA CAGGCTACCC GTTAGCAGTG 
AAACTGGGCA CCATTACGCC AGATGGAGGC GATGTATATT CCTATGATGA GGATGATATG 
GTTCTGGACC CCAACCTGGC AGAGCATCTC TCACACTTTG GCATTGACAT GATGAAGATG 
CAGAAGACGG ATAAGACCAT GACAGAACTG GAAATTGATA TGAACCAACG AATCGGAGAG 
TGGGAGGTGA TCCAGGAATC AGGTGTACAA TTGCAGCCCC TCTATGGTTC GGGGTACACT 
GGCATCCAGA ACCTCGGAAA CAGCTGCTAC CTCAATTCAA CCATGCAAGT CATCTTCAGC 
ATCCCGGCCT TCCAGCGCAA ATACGTAGAA CGTATGGAGC AGATTTTCCA GAAATCACCT 
GCTGATCCAA CGCAAGACTT CAACACCCAA GTAGCCAAGC TTGGAAATGG CATGTTATCT 
GGGGAATACT CAAAGCCAGC TGTAGAAAAA GACAATGACA CAGCTCCAGA ACATAAGGGT 
CTTCAGGATG GGATTGCTCC GAGAATGTTT AAGGCACTTG TTGGGAAGGG ACACCCAGAG 
TTTTCCACCA ACAGGCAGCA AGATGCTCAG GAATTCTTCC TGCACTTTAT AAATATGGTT 
GAGAGGAACT GCCGCAGCTC ATCCAACCCC AATGAAGTTT TCCGTTTCCT AGTGGAAGAG 
AAGATTAAGT GTCTGGTGTC TCAGAAAGTG AAGTATACAC AGAGGGTTGA GTACATCCTG 
CAACTTCCTG TGCCCATGGA GGCTGCACTC AATAAGGACG AGCTGTCTGC ATATGAGCAA 
TGCCGGCTCC AGGCTGAACG TGAGCATCGC CCCCCGCCAG AACTAGTCCG GGCACGGATC 
CCATTCACAT CATGTTTGGA GGCATTTGCT GCCCCTGAGC AGCTCGATGA ATTCTGGAGC 
GCCGCCCTTC AGGCCAAATC CAATGCACTT AAGACGTCCC GCTTTGCCTC ATTTCCAGAT 
TACTTGGTCA TTCAAATCAA GAAGTTTACT TTTGGCCTTG ACTGGGTACC CAAGAAACTG 
GATGTCTCTA TTGATGTCCC AGATGAATTG GATCTTTCTG AGTTTCGGGG TGGGGGGCTT 
CAAGGGGGGG AAGAGGAGCT CCCAGATGTT GCCCCCCCAT TGGTCACACC CGATGAGCCC 
AAAGGTAGCC TTGGTTTCTA TGGCCTCGAC GATGACGACT CGTACTGCTC CCCTCACTTC 
TCCTCTCCGA CATCTCCAGT GCTGGACGAG TCAGTAGTCA CGCAGTTGGT AGAGATGGGA 
TTCCCAGCCG AGGCGTGCCG CAAAGCTGTG TATTATACGG GCAACAGCGG GGCAGAGGCT 
GCAATGACGT GGGTGATGTC GCACATGGAT GACCCAGACT TTGCCTTGCC ACTGTCTGGG 
GCCAGCGCAC CCTCATCAGT TACTACCGGG GGGGATCCAC CCTCTGAGGA ACACGTAGCA 
ACAATAATAT CCATGGGCTT CTCCCAGGAG CAAGCAGTGA AAGCCCTCCG TGCCACTAAC 
AACAACTTGG AACGTGCTGT CGACTGGATC TTCTCTCACA TTGATGATTT GGATGCTGAG 
GCTGCCATGG ATTTATCTGA GGGTCGCTCT GCTGCAGAGT CTGTGTCTGA GAGTCTCCCT 
GCTGCGGGCC CTCGTGTGAG GGATGGAGCT GGGAGATATG AGCTTTTTGC CTTCATCAGT 
CACATGGGGA CATCCACCAT GTGCGGTCAC TACGTGTGTC ATATAAAGAA GGAGGGAAGG 
TGGGTGATCT ACAATGACCA GAAAGTCTGC GCTTCAGAGA AGCCTCCCAA AGACCTGGGT 
TACATTTACT TCTACCAGCG TATACAGAGT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

usp5 ( XM_012966040.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012821494.1 Xenopus tropicalis ubiquitin specific peptidase 5 (usp5), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa31425
Clone ID Related Accession (Same CDS sequence)	XM_012966040.2 , XM_012966040.3
Accession Version	XM_012966040.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2568bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	ubiquitin carboxyl-terminal hydrolase 5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012966040.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTGATG TGAAAGATGC TCTGCTCTCT GTGCTACCGA CCATTCGGCT ACCTCGGGCT 
GGGGACCGAG TGCACAAGGA TGAGTGTGCA TATACCTTCC ATACTCCGGA GACAGACAGC 
GGATTATATA TTTGTATGAA TACATTTTTG GGCTTTGGGA GGCCCTATGT TGAGCGGCAA 
TACAACAAGA CTGGGCAGAG AGCCTTTCTG CATCTTAAGA GGACTGCTAA AATGAAATCT 
GAGGAATCTA ATTCCGGCAC AGGAGACCCC CCACGCAAGA AGCCGACACG CTTAGCCATT 
GGAATGGAGG GCGGCTTTGA CATGAGTGAG GAACAGTGTG AATATGAGGA GGTGGTGAAG 
TTGGTCATTC TGCCTGAGTT CCTAGAGATT CCACGGGATG GACTGTTGGG GCTCCCGGAG 
ATGGTCAGGG ATCGGGTGTC GTCTGCTATT GATGCCATAC TGTCTGCTGA CTCCGCCTCC 
CGGAAACAGG AAATTCAGGC CTGGGACGGG GAAGTGAGGC AGGTTTCCAA ACATGCGTTC 
AACCTCCAGC AGCTTGAGGG GGTACCCCGT ATCCCACCTT GTGGATGGAA GTGCAGTGTC 
TGTGATCTGC GGGAGAATCT GTGGCTCAAT ATGACAGACG GCGCCATCCT CTGCGGAAGA 
CGTTACTTTG ATGGCAGCGG AGGGAATAAC CACGCGTTAC AGCACTACCA AGAAACAGGC 
TACCCGTTAG CAGTGAAACT GGGCACCATT ACGCCAGATG GAGGCGATGT ATATTCCTAT 
GATGAGGATG ATATGGTTCT GGACCCCAAC CTGGCAGAGC ATCTCTCACA CTTTGGCATT 
GACATGATGA AGATGCAGAA GACGGATAAG ACCATGACAG AACTGGAAAT TGATATGAAC 
CAACGAATCG GAGAGTGGGA GGTGATCCAG GAATCAGGTG TACAATTGCA GCCCCTCTAT 
GGTTCGGGGT ACACTGGCAT CCAGAACCTC GGAAACAGCT GCTACCTCAA TTCAACCATG 
CAAGTCATCT TCAGCATCCC GGCCTTCCAG CGCAAATACG TAGAACGTAT GGAGCAGATT 
TTCCAGAAAT CACCTGCTGA TCCAACGCAA GACTTCAACA CCCAAGTAGC CAAGCTTGGA 
AATGGCATGT TATCTGGGGA ATACTCAAAG CCAGCTGTAG AAAAAGACAA TGACACAGCT 
CCAGAACATA AGGGTCTTCA GGATGGGATT GCTCCGAGAA TGTTTAAGGC ACTTGTTGGG 
AAGGGACACC CAGAGTTTTC CACCAACAGG CAGCAAGATG CTCAGGAATT CTTCCTGCAC 
TTTATAAATA TGGTTGAGAG GAACTGCCGC AGCTCATCCA ACCCCAATGA AGTTTTCCGT 
TTCCTAGTGG AAGAGAAGAT TAAGTGTCTG GTGTCTCAGA AAGTGAAGTA TACACAGAGG 
GTTGAGTACA TCCTGCAACT TCCTGTGCCC ATGGAGGCTG CACTCAATAA GGACGAGCTG 
TCTGCATATG AGCAATGCCG GCTCCAGGCT GAACGTGAGC ATCGCCCCCC GCCAGAACTA 
GTCCGGGCAC GGATCCCATT CACATCATGT TTGGAGGCAT TTGCTGCCCC TGAGCAGCTC 
GATGAATTCT GGAGCGCCGC CCTTCAGGCC AAATCCAATG CACTTAAGAC GTCCCGCTTT 
GCCTCATTTC CAGATTACTT GGTCATTCAA ATCAAGAAGT TTACTTTTGG CCTTGACTGG 
GTACCCAAGA AACTGGATGT CTCTATTGAT GTCCCAGATG AATTGGATCT TTCTGAGTTT 
CGGGGTGGGG GGCTTCAAGG GGGGGAAGAG GAGCTCCCAG ATGTTGCCCC CCCATTGGTC 
ACACCCGATG AGCCCAAAGG TAGCCTTGGT TTCTATGGCC TCGACGATGA CGACTCGTAC 
TGCTCCCCTC ACTTCTCCTC TCCGACATCT CCAGTGCTGG ACGAGTCAGT AGTCACGCAG 
TTGGTAGAGA TGGGATTCCC AGCCGAGGCG TGCCGCAAAG CTGTGTATTA TACGGGCAAC 
AGCGGGGCAG AGGCTGCAAT GACGTGGGTG ATGTCGCACA TGGATGACCC AGACTTTGCC 
TTGCCACTGT CTGGGGCCAG CGCACCCTCA TCAGTTACTA CCGGGGGGGA TCCACCCTCT 
GAGGAACACG TAGCAACAAT AATATCCATG GGCTTCTCCC AGGAGCAAGC AGTGAAAGCC 
CTCCGTGCCA CTAACAACAA CTTGGAACGT GCTGTCGACT GGATCTTCTC TCACATTGAT 
GATTTGGATG CTGAGGCTGC CATGGATTTA TCTGAGGGTC GCTCTGCTGC AGAGTCTGTG 
TCTGAGAGTC TCCCTGCTGC GGGCCCTCGT GTGAGGGATG GAGCTGGGAG ATATGAGCTT 
TTTGCCTTCA TCAGTCACAT GGGGACATCC ACCATGTGCG GTCACTACGT GTGTCATATA 
AAGAAGGAGG GAAGGTGGGT GATCTACAAT GACCAGAAAG TCTGCGCTTC AGAGAAGCCT 
CCCAAAGACC TGGGTTACAT TTACTTCTAC CAGCGTATAC AGAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012821494.1
CDS	97..2664
Translation	MGDVKDALLSVLPTIRLPRAGDRVHKDECAYTFHTPETDSGLYICMNTFLGFGRPYVERQYNKTGQRAFLHLKRTAKMKSEESNSGTGDPPRKKPTRLAIGMEGGFDMSEEQCEYEEVVKLVILPEFLEIPRDGLLGLPEMVRDRVSSAIDAILSADSASRKQEIQAWDGEVRQVSKHAFNLQQLEGVPRIPPCGWKCSVCDLRENLWLNMTDGAILCGRRYFDGSGGNNHALQHYQETGYPLAVKLGTITPDGGDVYSYDEDDMVLDPNLAEHLSHFGIDMMKMQKTDKTMTELEIDMNQRIGEWEVIQESGVQLQPLYGSGYTGIQNLGNSCYLNSTMQVIFSIPAFQRKYVERMEQIFQKSPADPTQDFNTQVAKLGNGMLSGEYSKPAVEKDNDTAPEHKGLQDGIAPRMFKALVGKGHPEFSTNRQQDAQEFFLHFINMVERNCRSSSNPNEVFRFLVEEKIKCLVSQKVKYTQRVEYILQLPVPMEAALNKDELSAYEQCRLQAEREHRPPPELVRARIPFTSCLEAFAAPEQLDEFWSAALQAKSNALKTSRFASFPDYLVIQIKKFTFGLDWVPKKLDVSIDVPDELDLSEFRGGGLQGGEEELPDVAPPLVTPDEPKGSLGFYGLDDDDSYCSPHFSSPTSPVLDESVVTQLVEMGFPAEACRKAVYYTGNSGAEAAMTWVMSHMDDPDFALPLSGASAPSSVTTGGDPPSEEHVATIISMGFSQEQAVKALRATNNNLERAVDWIFSHIDDLDAEAAMDLSEGRSAAESVSESLPAAGPRVRDGAGRYELFAFISHMGTSTMCGHYVCHIKKEGRWVIYNDQKVCASEKPPKDLGYIYFYQRIQS

Target ORF information:

RefSeq Version	XM_012966040.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis ubiquitin specific peptidase 5 (usp5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012966040.3

ORF Insert Sequence:

ATGGGTGATG TGAAAGATGC TCTGCTCTCT GTGCTACCGA CCATTCGGCT ACCTCGGGCT 
GGGGACCGAG TGCACAAGGA TGAGTGTGCA TATACCTTCC ATACTCCGGA GACAGACAGC 
GGATTATATA TTTGTATGAA TACATTTTTG GGCTTTGGGA GGCCCTATGT TGAGCGGCAA 
TACAACAAGA CTGGGCAGAG AGCCTTTCTG CATCTTAAGA GGACTGCTAA AATGAAATCT 
GAGGAATCTA ATTCCGGCAC AGGAGACCCC CCACGCAAGA AGCCGACACG CTTAGCCATT 
GGAATGGAGG GCGGCTTTGA CATGAGTGAG GAACAGTGTG AATATGAGGA GGTGGTGAAG 
TTGGTCATTC TGCCTGAGTT CCTAGAGATT CCACGGGATG GACTGTTGGG GCTCCCGGAG 
ATGGTCAGGG ATCGGGTGTC GTCTGCTATT GATGCCATAC TGTCTGCTGA CTCCGCCTCC 
CGGAAACAGG AAATTCAGGC CTGGGACGGG GAAGTGAGGC AGGTTTCCAA ACATGCGTTC 
AACCTCCAGC AGCTTGAGGG GGTACCCCGT ATCCCACCTT GTGGATGGAA GTGCAGTGTC 
TGTGATCTGC GGGAGAATCT GTGGCTCAAT ATGACAGACG GCGCCATCCT CTGCGGAAGA 
CGTTACTTTG ATGGCAGCGG AGGGAATAAC CACGCGTTAC AGCACTACCA AGAAACAGGC 
TACCCGTTAG CAGTGAAACT GGGCACCATT ACGCCAGATG GAGGCGATGT ATATTCCTAT 
GATGAGGATG ATATGGTTCT GGACCCCAAC CTGGCAGAGC ATCTCTCACA CTTTGGCATT 
GACATGATGA AGATGCAGAA GACGGATAAG ACCATGACAG AACTGGAAAT TGATATGAAC 
CAACGAATCG GAGAGTGGGA GGTGATCCAG GAATCAGGTG TACAATTGCA GCCCCTCTAT 
GGTTCGGGGT ACACTGGCAT CCAGAACCTC GGAAACAGCT GCTACCTCAA TTCAACCATG 
CAAGTCATCT TCAGCATCCC GGCCTTCCAG CGCAAATACG TAGAACGTAT GGAGCAGATT 
TTCCAGAAAT CACCTGCTGA TCCAACGCAA GACTTCAACA CCCAAGTAGC CAAGCTTGGA 
AATGGCATGT TATCTGGGGA ATACTCAAAG CCAGCTGTAG AAAAAGACAA TGACACAGCT 
CCAGAACATA AGGGTCTTCA GGATGGGATT GCTCCGAGAA TGTTTAAGGC ACTTGTTGGG 
AAGGGACACC CAGAGTTTTC CACCAACAGG CAGCAAGATG CTCAGGAATT CTTCCTGCAC 
TTTATAAATA TGGTTGAGAG GAACTGCCGC AGCTCATCCA ACCCCAATGA AGTTTTCCGT 
TTCCTAGTGG AAGAGAAGAT TAAGTGTCTG GTGTCTCAGA AAGTGAAGTA TACACAGAGG 
GTTGAGTACA TCCTGCAACT TCCTGTGCCC ATGGAGGCTG CACTCAATAA GGACGAGCTG 
TCTGCATATG AGCAATGCCG GCTCCAGGCT GAACGTGAGC ATCGCCCCCC GCCAGAACTA 
GTCCGGGCAC GGATCCCATT CACATCATGT TTGGAGGCAT TTGCTGCCCC TGAGCAGCTC 
GATGAATTCT GGAGCGCCGC CCTTCAGGCC AAATCCAATG CACTTAAGAC GTCCCGCTTT 
GCCTCATTTC CAGATTACTT GGTCATTCAA ATCAAGAAGT TTACTTTTGG CCTTGACTGG 
GTACCCAAGA AACTGGATGT CTCTATTGAT GTCCCAGATG AATTGGATCT TTCTGAGTTT 
CGGGGTGGGG GGCTTCAAGG GGGGGAAGAG GAGCTCCCAG ATGTTGCCCC CCCATTGGTC 
ACACCCGATG AGCCCAAAGG TAGCCTTGGT TTCTATGGCC TCGACGATGA CGACTCGTAC 
TGCTCCCCTC ACTTCTCCTC TCCGACATCT CCAGTGCTGG ACGAGTCAGT AGTCACGCAG 
TTGGTAGAGA TGGGATTCCC AGCCGAGGCG TGCCGCAAAG CTGTGTATTA TACGGGCAAC 
AGCGGGGCAG AGGCTGCAAT GACGTGGGTG ATGTCGCACA TGGATGACCC AGACTTTGCC 
TTGCCACTGT CTGGGGCCAG CGCACCCTCA TCAGTTACTA CCGGGGGGGA TCCACCCTCT 
GAGGAACACG TAGCAACAAT AATATCCATG GGCTTCTCCC AGGAGCAAGC AGTGAAAGCC 
CTCCGTGCCA CTAACAACAA CTTGGAACGT GCTGTCGACT GGATCTTCTC TCACATTGAT 
GATTTGGATG CTGAGGCTGC CATGGATTTA TCTGAGGGTC GCTCTGCTGC AGAGTCTGTG 
TCTGAGAGTC TCCCTGCTGC GGGCCCTCGT GTGAGGGATG GAGCTGGGAG ATATGAGCTT 
TTTGCCTTCA TCAGTCACAT GGGGACATCC ACCATGTGCG GTCACTACGT GTGTCATATA 
AAGAAGGAGG GAAGGTGGGT GATCTACAAT GACCAGAAAG TCTGCGCTTC AGAGAAGCCT 
CCCAAAGACC TGGGTTACAT TTACTTCTAC CAGCGTATAC AGAGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)
Klein SL,Strausberg RL,Wagner L,Pontius J,Clifton SW,Richardson P

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usp5 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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