USP5 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	USP5
Entrez Gene ID	8078
Full Name	ubiquitin specific peptidase 5
Synonyms	ISOT
General protein information	Preferred Names ubiquitin specific peptidase 5 Names ubiquitin carboxyl-terminal hydrolase 5 deubiquitinating enzyme 5 isopeptidase T testicular tissue protein Li 218 ubiquitin isopeptidase T ubiquitin specific protease 5 (isopeptidase T) ubiquitin thioesterase 5 ubiquitin thiolesterase 5 ubiquitin-specific protease-5 (ubiquitin isopeptidase T) ubiquitin-specific-processing protease 5
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 12 Map Location: 12p13.31
Summary	Ubiquitin (see MIM 191339)-dependent proteolysis is a complex pathway of protein metabolism implicated in such diverse cellular functions as maintenance of chromatin structure, receptor function, and degradation of abnormal proteins. A late step of the process involves disassembly of the polyubiquitin chains on degraded proteins into ubiquitin monomers. USP5 disassembles branched polyubiquitin chains by a sequential exo mechanism, starting at the proximal end of the chain (Wilkinson et al., 1995 [PubMed 7578059]).[supplied by OMIM, Mar 2010].
Disorder MIM:	601447

mRNA and Protein(s)

mRNA	Protein	Name
NM_003481.2	NP_003472.2	ubiquitin carboxyl-terminal hydrolase 5 isoform 2
NM_001098536.1	NP_001092006.1	ubiquitin carboxyl-terminal hydrolase 5 isoform 1
NM_003481.3	NP_003472.2	ubiquitin carboxyl-terminal hydrolase 5 isoform 2
NM_001098536.2	NP_001092006.1	ubiquitin carboxyl-terminal hydrolase 5 isoform 1

Pathways from BioSystems

Homologies

Homo sapiens (human)	USP5	NP_001092006.1
Mus musculus (house mouse)	Usp5	NP_038728.1
Danio rerio (zebrafish)	usp5	NP_999920.2
Drosophila melanogaster (fruit fly)	CG12082	NP_647773.1
Pan troglodytes (chimpanzee)	USP5	XP_508970.4
Bos taurus (cattle)	USP5	NP_001178985.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012248	XP_320294.3
	USP5	XP_001110639.1
Canis lupus familiaris (dog)	USP5	XP_543845.3
Rattus norvegicus (Norway rat)	Usp5	NP_001100089.1
Gallus gallus (chicken)	USP5	XP_003640490.1
Oryza sativa (rice)	Os01g0177200	NP_001042185.1
Arabidopsis thaliana (thale cress)	UBP14	NP_566666.2
Xenopus tropicalis (tropical clawed frog)	usp5	NP_001116956.1

Gene Ontology
Bibliography

Related articles in PubMed

USP5 promotes tumorigenesis and progression of pancreatic cancer by stabilizing FoxM1 protein.
Li XY, Wu HY, Mao XF, Jiang LX, Wang YX
Biochemical and biophysical research communications492(1)48-54(2017 10)

Inhibition of the deubiquitinase USP5 leads to c-Maf protein degradation and myeloma cell apoptosis.
Wang S, Juan J, Zhang Z, Du Y, Xu Y, Tong J, Cao B, Moran MF, Zeng Y, Mao X
Cell death & disease8(9)e3058(2017 Sep)

Smurf1 represses TNF-? production through ubiquitination and destabilization of USP5.
Qian G, Ren Y, Zuo Y, Yuan Y, Zhao P, Wang X, Cheng Q, Liu J, Zhang L, Guo T, Liu C, Zheng H
Biochemical and biophysical research communications474(3)491-496(2016 06)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

study demonstrates that USP5 plays a critical role in tumorigenesis and progression of pancreatic cancer by stabilizing FoxM1 protein, and provides a rationale for USP5 being a potential therapeutic approach against pancreatic ductal adenocarcinoma
Title: USP5 promotes tumorigenesis and progression of pancreatic cancer by stabilizing FoxM1 protein.

Taken together, our study for the first time clarified that the E3 ligase Smurf1 regulates USP5 protein stability and USP5-mediated TNF-alpha production through the ubiquitin proteasome pathway.
Title: Smurf1 represses TNF-? production through ubiquitination and destabilization of USP5.

Used IM-MS analysis to study conformational flexibility of the multidomain deubiquitinating enzyme ubiquitin specific protease 5 (USP5).
Title: Ion mobility-mass spectrometry reveals conformational flexibility in the deubiquitinating enzyme USP5.

Charge-state distribution and ion mobility analysis revealed that both mono- and tetra-ubiquitin bound to the compact conformation of USP5 only.
Title: Structural insights into interactions between ubiquitin specific protease 5 and its polyubiquitin substrates by mass spectrometry and ion mobility spectrometry.

A ubiquitin shuttle DC-UbP reconciles protein ubiquitination and deubiquitination via linking UbE1 and USP5 enzymes.
Title: A ubiquitin shuttle DC-UbP/UBTD2 reconciles protein ubiquitination and deubiquitination via linking UbE1 and USP5 enzymes.

The following USP5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the USP5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu30654	NM_003481.3 Latest version!	Homo sapiens ubiquitin specific peptidase 5 (USP5), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu31063	NM_001098536.2 Latest version!	Homo sapiens ubiquitin specific peptidase 5 (USP5), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
View Old Accession Versions >>
OHu30654	NM_003481.2	Homo sapiens ubiquitin specific peptidase 5 (USP5), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu31063	NM_001098536.1	Homo sapiens ubiquitin specific peptidase 5 (USP5), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

USP5 ( NM_003481.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_003472.2 Homo sapiens ubiquitin specific peptidase 5 (USP5), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu30654
Clone ID Related Accession (Same CDS sequence)	NM_003481.2 , NM_003481.3
Accession Version	NM_003481.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2508bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-10
Organism	Homo sapiens(human)
Product	ubiquitin carboxyl-terminal hydrolase 5 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CN276110.1, BC004889.2 and AI289395.1. On Jun 8, 2007 this sequence version replaced NM_003481.1. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 3' coding region, compared to variant 1, resulting in a shorter protein (isoform 2). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163657.319952.1, BC004889.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END##

ATGGCGGAGC TGAGTGAGGA GGCGCTGCTG TCAGTATTAC CGACGATCCG GGTCCCTAAG 
GCTGGAGACC GGGTCCACAA AGACGAGTGC GCCTTCTCCT TCGACACGCC GGAGTCTGAG 
GGGGGCCTCT ACATCTGTAT GAACACGTTT CTGGGCTTTG GGAAACAGTA TGTGGAGAGA 
CATTTCAATA AGACCGGCCA GCGAGTCTAC TTGCACCTCC GGCGGACCCG GCGCCCGAAA 
GAGGAGGACC CTGCTACAGG CACTGGAGAC CCACCCCGGA AGAAGCCCAC GCGGCTGGCT 
ATTGGTGTTG AAGGCGGATT TGACCTTAGC GAGGAGAAGT TTGAATTAGA CGAGGATGTG 
AAGATTGTCA TTTTGCCAGA TTACCTGGAG ATTGCCCGGG ATGGACTGGG GGGACTGCCT 
GACATTGTCA GAGATCGGGT GACCAGTGCA GTGGAGGCCC TACTGTCGGC CGACTCAGCC 
TCCCGCAAGC AGGAGGTGCA GGCATGGGAT GGGGAAGTAC GGCAGGTGTC TAAGCATGCC 
TTCAGCCTCA AGCAGTTGGA CAACCCTGCT CGAATCCCTC CCTGTGGCTG GAAGTGCTCC 
AAGTGTGACA TGAGAGAGAA CCTGTGGCTC AACCTGACTG ATGGCTCCAT CCTCTGTGGG 
CGACGCTACT TCGATGGCAG TGGGGGCAAC AACCACGCTG TGGAGCACTA CCGAGAGACA 
GGCTACCCGT TAGCTGTCAA GCTGGGCACC ATCACCCCTG ATGGAGCTGA CGTGTACTCA 
TATGATGAGG ATGACATGGT CCTGGACCCC AGCCTGGCTG AGCACCTGTC CCACTTCGGC 
ATCGACATGC TGAAGATGCA GAAGACAGAC AAGACGATGA CTGAGTTGGA GATAGACATG 
AACCAGCGGA TTGGTGAATG GGAGCTGATC CAGGAGTCAG GTGTGCCACT CAAGCCCCTG 
TTTGGGCCTG GCTACACAGG CATCCGGAAC CTGGGTAACA GCTGCTACCT CAACTCTGTG 
GTCCAGGTGC TCTTCAGCAT CCCTGACTTC CAGAGGAAGT ATGTGGATAA GCTGGAGAAG 
ATCTTCCAGA ATGCCCCGAC GGACCCTACC CAGGATTTCA GCACCCAGGT GGCCAAGCTG 
GGCCATGGCC TTCTCTCCGG GGAGTATTCC AAGCCAGTAC CGGAGTCGGG CGATGGGGAG 
CGGGTGCCAG AACAGAAGGA AGTTCAAGAT GGCATTGCCC CTCGGATGTT CAAGGCCCTC 
ATCGGCAAGG GCCACCCTGA ATTCTCCACC AACCGGCAGC AGGATGCCCA GGAGTTCTTC 
CTTCACCTTA TCAACATGGT GGAGAGGAAT TGCCGGAGCT CTGAAAATCC TAATGAAGTG 
TTCCGCTTCT TGGTGGAGGA AAAGATCAAG TGCCTGGCCA CAGAGAAGGT GAAGTACACC 
CAGCGAGTTG ACTACATCAT GCAGCTGCCT GTGCCCATGG ATGCAGCCCT TAACAAAGAG 
GAGCTTCTGG AGTACGAGGA GAAGAAGCGG CAAGCCGAAG AGGAGAAGAT GGCACTGCCA 
GAACTGGTTC GGGCCCAGGT GCCCTTCAGC TCTTGCCTGG AGGCCTACGG GGCCCCTGAG 
CAGGTCGATG ACTTCTGGAG CACGGCCCTG CAGGCCAAGT CAGTAGCTGT CAAGACCACA 
CGATTTGCCT CATTCCCTGA CTACCTGGTC ATCCAGATCA AGAAGTTCAC CTTCGGCTTA 
GACTGGGTGC CCAAGAAACT GGATGTGTCC ATCGAGATGC CAGAGGAGCT CGACATCTCC 
CAGTTGAGGG GCACAGGGCT GCAGCCCGGA GAGGAGGAGC TGCCAGACAT TGCCCCACCC 
CTGGTCACTC CGGATGAGCC CAAAGCGCCC ATGCTGGATG AATCAGTCAT CATCCAGCTG 
GTGGAGATGG GATTCCCTAT GGACGCCTGC CGCAAAGCTG TCTACTACAC GGGCAACAGC 
GGGGCTGAGG CCGCCATGAA CTGGGTCATG TCACACATGG ATGATCCAGA TTTTGCAAAC 
CCCCTCATCC TGCCTGGCTC TAGTGGGCCG GGCTCCACAA GCGCAGCAGC CGACCCCCCT 
CCTGAGGACT GTGTGACCAC CATTGTCTCC ATGGGCTTCT CCCGGGACCA GGCCTTGAAA 
GCGCTGCGGG CCACGAACAA TAGTTTAGAA CGGGCTGTGG ACTGGATCTT CAGTCACATT 
GACGACCTGG ATGCTGAAGC TGCCATGGAC ATCTCAGAGG GCCGCTCAGC TGCCGACTCC 
ATCTCTGAGT CTGTGCCAGT GGGACCTAAA GTCCGGGATG GTCCTGGAAA GTATCAGCTC 
TTTGCCTTCA TTAGTCACAT GGGCACCTCT ACCATGTGTG GTCACTACGT CTGCCACATC 
AAGAAAGAAG GCAGATGGGT GATCTACAAT GACCAGAAAG TGTGTGCCTC CGAGAAGCCG 
CCCAAGGACC TGGGCTACAT CTACTTCTAC CAGAGAGTGG CCAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_003472.2
CDS	54..2561
Translation	MAELSEEALLSVLPTIRVPKAGDRVHKDECAFSFDTPESEGGLYICMNTFLGFGKQYVERHFNKTGQRVYLHLRRTRRPKEEDPATGTGDPPRKKPTRLAIGVEGGFDLSEEKFELDEDVKIVILPDYLEIARDGLGGLPDIVRDRVTSAVEALLSADSASRKQEVQAWDGEVRQVSKHAFSLKQLDNPARIPPCGWKCSKCDMRENLWLNLTDGSILCGRRYFDGSGGNNHAVEHYRETGYPLAVKLGTITPDGADVYSYDEDDMVLDPSLAEHLSHFGIDMLKMQKTDKTMTELEIDMNQRIGEWELIQESGVPLKPLFGPGYTGIRNLGNSCYLNSVVQVLFSIPDFQRKYVDKLEKIFQNAPTDPTQDFSTQVAKLGHGLLSGEYSKPVPESGDGERVPEQKEVQDGIAPRMFKALIGKGHPEFSTNRQQDAQEFFLHLINMVERNCRSSENPNEVFRFLVEEKIKCLATEKVKYTQRVDYIMQLPVPMDAALNKEELLEYEEKKRQAEEEKMALPELVRAQVPFSSCLEAYGAPEQVDDFWSTALQAKSVAVKTTRFASFPDYLVIQIKKFTFGLDWVPKKLDVSIEMPEELDISQLRGTGLQPGEEELPDIAPPLVTPDEPKAPMLDESVIIQLVEMGFPMDACRKAVYYTGNSGAEAAMNWVMSHMDDPDFANPLILPGSSGPGSTSAAADPPPEDCVTTIVSMGFSRDQALKALRATNNSLERAVDWIFSHIDDLDAEAAMDISEGRSAADSISESVPVGPKVRDGPGKYQLFAFISHMGTSTMCGHYVCHIKKEGRWVIYNDQKVCASEKPPKDLGYIYFYQRVAS

Target ORF information:

RefSeq Version	NM_003481.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 5 (USP5), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003481.2

ORF Insert Sequence:

ATGGCGGAGC TGAGTGAGGA GGCGCTGCTG TCAGTATTAC CGACGATCCG GGTCCCTAAG 
GCTGGAGACC GGGTCCACAA AGACGAGTGC GCCTTCTCCT TCGACACGCC GGAGTCTGAG 
GGGGGCCTCT ACATCTGTAT GAACACGTTT CTGGGCTTTG GGAAACAGTA TGTGGAGAGA 
CATTTCAATA AGACCGGCCA GCGAGTCTAC TTGCACCTCC GGCGGACCCG GCGCCCGAAA 
GAGGAGGACC CTGCTACAGG CACTGGAGAC CCACCCCGGA AGAAGCCCAC GCGGCTGGCT 
ATTGGTGTTG AAGGCGGATT TGACCTTAGC GAGGAGAAGT TTGAATTAGA CGAGGATGTG 
AAGATTGTCA TTTTGCCAGA TTACCTGGAG ATTGCCCGGG ATGGACTGGG GGGACTGCCT 
GACATTGTCA GAGATCGGGT GACCAGTGCA GTGGAGGCCC TACTGTCGGC CGACTCAGCC 
TCCCGCAAGC AGGAGGTGCA GGCATGGGAT GGGGAAGTAC GGCAGGTGTC TAAGCATGCC 
TTCAGCCTCA AGCAGTTGGA CAACCCTGCT CGAATCCCTC CCTGTGGCTG GAAGTGCTCC 
AAGTGTGACA TGAGAGAGAA CCTGTGGCTC AACCTGACTG ATGGCTCCAT CCTCTGTGGG 
CGACGCTACT TCGATGGCAG TGGGGGCAAC AACCACGCTG TGGAGCACTA CCGAGAGACA 
GGCTACCCGT TAGCTGTCAA GCTGGGCACC ATCACCCCTG ATGGAGCTGA CGTGTACTCA 
TATGATGAGG ATGACATGGT CCTGGACCCC AGCCTGGCTG AGCACCTGTC CCACTTCGGC 
ATCGACATGC TGAAGATGCA GAAGACAGAC AAGACGATGA CTGAGTTGGA GATAGACATG 
AACCAGCGGA TTGGTGAATG GGAGCTGATC CAGGAGTCAG GTGTGCCACT CAAGCCCCTG 
TTTGGGCCTG GCTACACAGG CATCCGGAAC CTGGGTAACA GCTGCTACCT CAACTCTGTG 
GTCCAGGTGC TCTTCAGCAT CCCTGACTTC CAGAGGAAGT ATGTGGATAA GCTGGAGAAG 
ATCTTCCAGA ATGCCCCGAC GGACCCTACC CAGGATTTCA GCACCCAGGT GGCCAAGCTG 
GGCCATGGCC TTCTCTCCGG GGAGTATTCC AAGCCAGTAC CGGAGTCGGG CGATGGGGAG 
CGGGTGCCAG AACAGAAGGA AGTTCAAGAT GGCATTGCCC CTCGGATGTT CAAGGCCCTC 
ATCGGCAAGG GCCACCCTGA ATTCTCCACC AACCGGCAGC AGGATGCCCA GGAGTTCTTC 
CTTCACCTTA TCAACATGGT GGAGAGGAAT TGCCGGAGCT CTGAAAATCC TAATGAAGTG 
TTCCGCTTCT TGGTGGAGGA AAAGATCAAG TGCCTGGCCA CAGAGAAGGT GAAGTACACC 
CAGCGAGTTG ACTACATCAT GCAGCTGCCT GTGCCCATGG ATGCAGCCCT TAACAAAGAG 
GAGCTTCTGG AGTACGAGGA GAAGAAGCGG CAAGCCGAAG AGGAGAAGAT GGCACTGCCA 
GAACTGGTTC GGGCCCAGGT GCCCTTCAGC TCTTGCCTGG AGGCCTACGG GGCCCCTGAG 
CAGGTCGATG ACTTCTGGAG CACGGCCCTG CAGGCCAAGT CAGTAGCTGT CAAGACCACA 
CGATTTGCCT CATTCCCTGA CTACCTGGTC ATCCAGATCA AGAAGTTCAC CTTCGGCTTA 
GACTGGGTGC CCAAGAAACT GGATGTGTCC ATCGAGATGC CAGAGGAGCT CGACATCTCC 
CAGTTGAGGG GCACAGGGCT GCAGCCCGGA GAGGAGGAGC TGCCAGACAT TGCCCCACCC 
CTGGTCACTC CGGATGAGCC CAAAGCGCCC ATGCTGGATG AATCAGTCAT CATCCAGCTG 
GTGGAGATGG GATTCCCTAT GGACGCCTGC CGCAAAGCTG TCTACTACAC GGGCAACAGC 
GGGGCTGAGG CCGCCATGAA CTGGGTCATG TCACACATGG ATGATCCAGA TTTTGCAAAC 
CCCCTCATCC TGCCTGGCTC TAGTGGGCCG GGCTCCACAA GCGCAGCAGC CGACCCCCCT 
CCTGAGGACT GTGTGACCAC CATTGTCTCC ATGGGCTTCT CCCGGGACCA GGCCTTGAAA 
GCGCTGCGGG CCACGAACAA TAGTTTAGAA CGGGCTGTGG ACTGGATCTT CAGTCACATT 
GACGACCTGG ATGCTGAAGC TGCCATGGAC ATCTCAGAGG GCCGCTCAGC TGCCGACTCC 
ATCTCTGAGT CTGTGCCAGT GGGACCTAAA GTCCGGGATG GTCCTGGAAA GTATCAGCTC 
TTTGCCTTCA TTAGTCACAT GGGCACCTCT ACCATGTGTG GTCACTACGT CTGCCACATC 
AAGAAAGAAG GCAGATGGGT GATCTACAAT GACCAGAAAG TGTGTGCCTC CGAGAAGCCG 
CCCAAGGACC TGGGCTACAT CTACTTCTAC CAGAGAGTGG CCAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP5 ( NM_003481.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_003472.2 Homo sapiens ubiquitin specific peptidase 5 (USP5), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu30654
Clone ID Related Accession (Same CDS sequence)	NM_003481.2 , NM_003481.3
Accession Version	NM_003481.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2508bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-15
Organism	Homo sapiens(human)
Product	ubiquitin carboxyl-terminal hydrolase 5 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC004889.2 and AI289395.1. On Jun 2, 2019 this sequence version replaced NM_003481.2. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 3' coding region, compared to variant 1, resulting in a shorter protein (isoform 2). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR7346977.848879.1, SRR1803613.85694.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END##

ATGGCGGAGC TGAGTGAGGA GGCGCTGCTG TCAGTATTAC CGACGATCCG GGTCCCTAAG 
GCTGGAGACC GGGTCCACAA AGACGAGTGC GCCTTCTCCT TCGACACGCC GGAGTCTGAG 
GGGGGCCTCT ACATCTGTAT GAACACGTTT CTGGGCTTTG GGAAACAGTA TGTGGAGAGA 
CATTTCAATA AGACCGGCCA GCGAGTCTAC TTGCACCTCC GGCGGACCCG GCGCCCGAAA 
GAGGAGGACC CTGCTACAGG CACTGGAGAC CCACCCCGGA AGAAGCCCAC GCGGCTGGCT 
ATTGGTGTTG AAGGCGGATT TGACCTTAGC GAGGAGAAGT TTGAATTAGA CGAGGATGTG 
AAGATTGTCA TTTTGCCAGA TTACCTGGAG ATTGCCCGGG ATGGACTGGG GGGACTGCCT 
GACATTGTCA GAGATCGGGT GACCAGTGCA GTGGAGGCCC TACTGTCGGC CGACTCAGCC 
TCCCGCAAGC AGGAGGTGCA GGCATGGGAT GGGGAAGTAC GGCAGGTGTC TAAGCATGCC 
TTCAGCCTCA AGCAGTTGGA CAACCCTGCT CGAATCCCTC CCTGTGGCTG GAAGTGCTCC 
AAGTGTGACA TGAGAGAGAA CCTGTGGCTC AACCTGACTG ATGGCTCCAT CCTCTGTGGG 
CGACGCTACT TCGATGGCAG TGGGGGCAAC AACCACGCTG TGGAGCACTA CCGAGAGACA 
GGCTACCCGT TAGCTGTCAA GCTGGGCACC ATCACCCCTG ATGGAGCTGA CGTGTACTCA 
TATGATGAGG ATGACATGGT CCTGGACCCC AGCCTGGCTG AGCACCTGTC CCACTTCGGC 
ATCGACATGC TGAAGATGCA GAAGACAGAC AAGACGATGA CTGAGTTGGA GATAGACATG 
AACCAGCGGA TTGGTGAATG GGAGCTGATC CAGGAGTCAG GTGTGCCACT CAAGCCCCTG 
TTTGGGCCTG GCTACACAGG CATCCGGAAC CTGGGTAACA GCTGCTACCT CAACTCTGTG 
GTCCAGGTGC TCTTCAGCAT CCCTGACTTC CAGAGGAAGT ATGTGGATAA GCTGGAGAAG 
ATCTTCCAGA ATGCCCCGAC GGACCCTACC CAGGATTTCA GCACCCAGGT GGCCAAGCTG 
GGCCATGGCC TTCTCTCCGG GGAGTATTCC AAGCCAGTAC CGGAGTCGGG CGATGGGGAG 
CGGGTGCCAG AACAGAAGGA AGTTCAAGAT GGCATTGCCC CTCGGATGTT CAAGGCCCTC 
ATCGGCAAGG GCCACCCTGA ATTCTCCACC AACCGGCAGC AGGATGCCCA GGAGTTCTTC 
CTTCACCTTA TCAACATGGT GGAGAGGAAT TGCCGGAGCT CTGAAAATCC TAATGAAGTG 
TTCCGCTTCT TGGTGGAGGA AAAGATCAAG TGCCTGGCCA CAGAGAAGGT GAAGTACACC 
CAGCGAGTTG ACTACATCAT GCAGCTGCCT GTGCCCATGG ATGCAGCCCT TAACAAAGAG 
GAGCTTCTGG AGTACGAGGA GAAGAAGCGG CAAGCCGAAG AGGAGAAGAT GGCACTGCCA 
GAACTGGTTC GGGCCCAGGT GCCCTTCAGC TCTTGCCTGG AGGCCTACGG GGCCCCTGAG 
CAGGTCGATG ACTTCTGGAG CACGGCCCTG CAGGCCAAGT CAGTAGCTGT CAAGACCACA 
CGATTTGCCT CATTCCCTGA CTACCTGGTC ATCCAGATCA AGAAGTTCAC CTTCGGCTTA 
GACTGGGTGC CCAAGAAACT GGATGTGTCC ATCGAGATGC CAGAGGAGCT CGACATCTCC 
CAGTTGAGGG GCACAGGGCT GCAGCCCGGA GAGGAGGAGC TGCCAGACAT TGCCCCACCC 
CTGGTCACTC CGGATGAGCC CAAAGCGCCC ATGCTGGATG AATCAGTCAT CATCCAGCTG 
GTGGAGATGG GATTCCCTAT GGACGCCTGC CGCAAAGCTG TCTACTACAC GGGCAACAGC 
GGGGCTGAGG CCGCCATGAA CTGGGTCATG TCACACATGG ATGATCCAGA TTTTGCAAAC 
CCCCTCATCC TGCCTGGCTC TAGTGGGCCG GGCTCCACAA GCGCAGCAGC CGACCCCCCT 
CCTGAGGACT GTGTGACCAC CATTGTCTCC ATGGGCTTCT CCCGGGACCA GGCCTTGAAA 
GCGCTGCGGG CCACGAACAA TAGTTTAGAA CGGGCTGTGG ACTGGATCTT CAGTCACATT 
GACGACCTGG ATGCTGAAGC TGCCATGGAC ATCTCAGAGG GCCGCTCAGC TGCCGACTCC 
ATCTCTGAGT CTGTGCCAGT GGGACCTAAA GTCCGGGATG GTCCTGGAAA GTATCAGCTC 
TTTGCCTTCA TTAGTCACAT GGGCACCTCT ACCATGTGTG GTCACTACGT CTGCCACATC 
AAGAAAGAAG GCAGATGGGT GATCTACAAT GACCAGAAAG TGTGTGCCTC CGAGAAGCCG 
CCCAAGGACC TGGGCTACAT CTACTTCTAC CAGAGAGTGG CCAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_003472.2
CDS	31..2538
Translation	MAELSEEALLSVLPTIRVPKAGDRVHKDECAFSFDTPESEGGLYICMNTFLGFGKQYVERHFNKTGQRVYLHLRRTRRPKEEDPATGTGDPPRKKPTRLAIGVEGGFDLSEEKFELDEDVKIVILPDYLEIARDGLGGLPDIVRDRVTSAVEALLSADSASRKQEVQAWDGEVRQVSKHAFSLKQLDNPARIPPCGWKCSKCDMRENLWLNLTDGSILCGRRYFDGSGGNNHAVEHYRETGYPLAVKLGTITPDGADVYSYDEDDMVLDPSLAEHLSHFGIDMLKMQKTDKTMTELEIDMNQRIGEWELIQESGVPLKPLFGPGYTGIRNLGNSCYLNSVVQVLFSIPDFQRKYVDKLEKIFQNAPTDPTQDFSTQVAKLGHGLLSGEYSKPVPESGDGERVPEQKEVQDGIAPRMFKALIGKGHPEFSTNRQQDAQEFFLHLINMVERNCRSSENPNEVFRFLVEEKIKCLATEKVKYTQRVDYIMQLPVPMDAALNKEELLEYEEKKRQAEEEKMALPELVRAQVPFSSCLEAYGAPEQVDDFWSTALQAKSVAVKTTRFASFPDYLVIQIKKFTFGLDWVPKKLDVSIEMPEELDISQLRGTGLQPGEEELPDIAPPLVTPDEPKAPMLDESVIIQLVEMGFPMDACRKAVYYTGNSGAEAAMNWVMSHMDDPDFANPLILPGSSGPGSTSAAADPPPEDCVTTIVSMGFSRDQALKALRATNNSLERAVDWIFSHIDDLDAEAAMDISEGRSAADSISESVPVGPKVRDGPGKYQLFAFISHMGTSTMCGHYVCHIKKEGRWVIYNDQKVCASEKPPKDLGYIYFYQRVAS

Target ORF information:

RefSeq Version	NM_003481.3
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 5 (USP5), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003481.3

ORF Insert Sequence:

ATGGCGGAGC TGAGTGAGGA GGCGCTGCTG TCAGTATTAC CGACGATCCG GGTCCCTAAG 
GCTGGAGACC GGGTCCACAA AGACGAGTGC GCCTTCTCCT TCGACACGCC GGAGTCTGAG 
GGGGGCCTCT ACATCTGTAT GAACACGTTT CTGGGCTTTG GGAAACAGTA TGTGGAGAGA 
CATTTCAATA AGACCGGCCA GCGAGTCTAC TTGCACCTCC GGCGGACCCG GCGCCCGAAA 
GAGGAGGACC CTGCTACAGG CACTGGAGAC CCACCCCGGA AGAAGCCCAC GCGGCTGGCT 
ATTGGTGTTG AAGGCGGATT TGACCTTAGC GAGGAGAAGT TTGAATTAGA CGAGGATGTG 
AAGATTGTCA TTTTGCCAGA TTACCTGGAG ATTGCCCGGG ATGGACTGGG GGGACTGCCT 
GACATTGTCA GAGATCGGGT GACCAGTGCA GTGGAGGCCC TACTGTCGGC CGACTCAGCC 
TCCCGCAAGC AGGAGGTGCA GGCATGGGAT GGGGAAGTAC GGCAGGTGTC TAAGCATGCC 
TTCAGCCTCA AGCAGTTGGA CAACCCTGCT CGAATCCCTC CCTGTGGCTG GAAGTGCTCC 
AAGTGTGACA TGAGAGAGAA CCTGTGGCTC AACCTGACTG ATGGCTCCAT CCTCTGTGGG 
CGACGCTACT TCGATGGCAG TGGGGGCAAC AACCACGCTG TGGAGCACTA CCGAGAGACA 
GGCTACCCGT TAGCTGTCAA GCTGGGCACC ATCACCCCTG ATGGAGCTGA CGTGTACTCA 
TATGATGAGG ATGACATGGT CCTGGACCCC AGCCTGGCTG AGCACCTGTC CCACTTCGGC 
ATCGACATGC TGAAGATGCA GAAGACAGAC AAGACGATGA CTGAGTTGGA GATAGACATG 
AACCAGCGGA TTGGTGAATG GGAGCTGATC CAGGAGTCAG GTGTGCCACT CAAGCCCCTG 
TTTGGGCCTG GCTACACAGG CATCCGGAAC CTGGGTAACA GCTGCTACCT CAACTCTGTG 
GTCCAGGTGC TCTTCAGCAT CCCTGACTTC CAGAGGAAGT ATGTGGATAA GCTGGAGAAG 
ATCTTCCAGA ATGCCCCGAC GGACCCTACC CAGGATTTCA GCACCCAGGT GGCCAAGCTG 
GGCCATGGCC TTCTCTCCGG GGAGTATTCC AAGCCAGTAC CGGAGTCGGG CGATGGGGAG 
CGGGTGCCAG AACAGAAGGA AGTTCAAGAT GGCATTGCCC CTCGGATGTT CAAGGCCCTC 
ATCGGCAAGG GCCACCCTGA ATTCTCCACC AACCGGCAGC AGGATGCCCA GGAGTTCTTC 
CTTCACCTTA TCAACATGGT GGAGAGGAAT TGCCGGAGCT CTGAAAATCC TAATGAAGTG 
TTCCGCTTCT TGGTGGAGGA AAAGATCAAG TGCCTGGCCA CAGAGAAGGT GAAGTACACC 
CAGCGAGTTG ACTACATCAT GCAGCTGCCT GTGCCCATGG ATGCAGCCCT TAACAAAGAG 
GAGCTTCTGG AGTACGAGGA GAAGAAGCGG CAAGCCGAAG AGGAGAAGAT GGCACTGCCA 
GAACTGGTTC GGGCCCAGGT GCCCTTCAGC TCTTGCCTGG AGGCCTACGG GGCCCCTGAG 
CAGGTCGATG ACTTCTGGAG CACGGCCCTG CAGGCCAAGT CAGTAGCTGT CAAGACCACA 
CGATTTGCCT CATTCCCTGA CTACCTGGTC ATCCAGATCA AGAAGTTCAC CTTCGGCTTA 
GACTGGGTGC CCAAGAAACT GGATGTGTCC ATCGAGATGC CAGAGGAGCT CGACATCTCC 
CAGTTGAGGG GCACAGGGCT GCAGCCCGGA GAGGAGGAGC TGCCAGACAT TGCCCCACCC 
CTGGTCACTC CGGATGAGCC CAAAGCGCCC ATGCTGGATG AATCAGTCAT CATCCAGCTG 
GTGGAGATGG GATTCCCTAT GGACGCCTGC CGCAAAGCTG TCTACTACAC GGGCAACAGC 
GGGGCTGAGG CCGCCATGAA CTGGGTCATG TCACACATGG ATGATCCAGA TTTTGCAAAC 
CCCCTCATCC TGCCTGGCTC TAGTGGGCCG GGCTCCACAA GCGCAGCAGC CGACCCCCCT 
CCTGAGGACT GTGTGACCAC CATTGTCTCC ATGGGCTTCT CCCGGGACCA GGCCTTGAAA 
GCGCTGCGGG CCACGAACAA TAGTTTAGAA CGGGCTGTGG ACTGGATCTT CAGTCACATT 
GACGACCTGG ATGCTGAAGC TGCCATGGAC ATCTCAGAGG GCCGCTCAGC TGCCGACTCC 
ATCTCTGAGT CTGTGCCAGT GGGACCTAAA GTCCGGGATG GTCCTGGAAA GTATCAGCTC 
TTTGCCTTCA TTAGTCACAT GGGCACCTCT ACCATGTGTG GTCACTACGT CTGCCACATC 
AAGAAAGAAG GCAGATGGGT GATCTACAAT GACCAGAAAG TGTGTGCCTC CGAGAAGCCG 
CCCAAGGACC TGGGCTACAT CTACTTCTAC CAGAGAGTGG CCAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP5 ( NM_001098536.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001092006.1 Homo sapiens ubiquitin specific peptidase 5 (USP5), transcript variant 1, mRNA.

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CloneID	OHu31063
Clone ID Related Accession (Same CDS sequence)	NM_001098536.1 , NM_001098536.2
Accession Version	NM_001098536.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2577bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-17
Organism	Homo sapiens(human)
Product	ubiquitin carboxyl-terminal hydrolase 5 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AU099996.1, U47927.1 and BC005139.2. Transcript Variant: This variant (1) represents the longer transcript and it encodes the longer protein (isoform 1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC005139.2, U47927.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END##

ATGGCGGAGC TGAGTGAGGA GGCGCTGCTG TCAGTATTAC CGACGATCCG GGTCCCTAAG 
GCTGGAGACC GGGTCCACAA AGACGAGTGC GCCTTCTCCT TCGACACGCC GGAGTCTGAG 
GGGGGCCTCT ACATCTGTAT GAACACGTTT CTGGGCTTTG GGAAACAGTA TGTGGAGAGA 
CATTTCAATA AGACCGGCCA GCGAGTCTAC TTGCACCTCC GGCGGACCCG GCGCCCGAAA 
GAGGAGGACC CTGCTACAGG CACTGGAGAC CCACCCCGGA AGAAGCCCAC GCGGCTGGCT 
ATTGGTGTTG AAGGCGGATT TGACCTTAGC GAGGAGAAGT TTGAATTAGA CGAGGATGTG 
AAGATTGTCA TTTTGCCAGA TTACCTGGAG ATTGCCCGGG ATGGACTGGG GGGACTGCCT 
GACATTGTCA GAGATCGGGT GACCAGTGCA GTGGAGGCCC TACTGTCGGC CGACTCAGCC 
TCCCGCAAGC AGGAGGTGCA GGCATGGGAT GGGGAAGTAC GGCAGGTGTC TAAGCATGCC 
TTCAGCCTCA AGCAGTTGGA CAACCCTGCT CGAATCCCTC CCTGTGGCTG GAAGTGCTCC 
AAGTGTGACA TGAGAGAGAA CCTGTGGCTC AACCTGACTG ATGGCTCCAT CCTCTGTGGG 
CGACGCTACT TCGATGGCAG TGGGGGCAAC AACCACGCTG TGGAGCACTA CCGAGAGACA 
GGCTACCCGT TAGCTGTCAA GCTGGGCACC ATCACCCCTG ATGGAGCTGA CGTGTACTCA 
TATGATGAGG ATGACATGGT CCTGGACCCC AGCCTGGCTG AGCACCTGTC CCACTTCGGC 
ATCGACATGC TGAAGATGCA GAAGACAGAC AAGACGATGA CTGAGTTGGA GATAGACATG 
AACCAGCGGA TTGGTGAATG GGAGCTGATC CAGGAGTCAG GTGTGCCACT CAAGCCCCTG 
TTTGGGCCTG GCTACACAGG CATCCGGAAC CTGGGTAACA GCTGCTACCT CAACTCTGTG 
GTCCAGGTGC TCTTCAGCAT CCCTGACTTC CAGAGGAAGT ATGTGGATAA GCTGGAGAAG 
ATCTTCCAGA ATGCCCCGAC GGACCCTACC CAGGATTTCA GCACCCAGGT GGCCAAGCTG 
GGCCATGGCC TTCTCTCCGG GGAGTATTCC AAGCCAGTAC CGGAGTCGGG CGATGGGGAG 
CGGGTGCCAG AACAGAAGGA AGTTCAAGAT GGCATTGCCC CTCGGATGTT CAAGGCCCTC 
ATCGGCAAGG GCCACCCTGA ATTCTCCACC AACCGGCAGC AGGATGCCCA GGAGTTCTTC 
CTTCACCTTA TCAACATGGT GGAGAGGAAT TGCCGGAGCT CTGAAAATCC TAATGAAGTG 
TTCCGCTTCT TGGTGGAGGA AAAGATCAAG TGCCTGGCCA CAGAGAAGGT GAAGTACACC 
CAGCGAGTTG ACTACATCAT GCAGCTGCCT GTGCCCATGG ATGCAGCCCT TAACAAAGAG 
GAGCTTCTGG AGTACGAGGA GAAGAAGCGG CAAGCCGAAG AGGAGAAGAT GGCACTGCCA 
GAACTGGTTC GGGCCCAGGT GCCCTTCAGC TCTTGCCTGG AGGCCTACGG GGCCCCTGAG 
CAGGTCGATG ACTTCTGGAG CACGGCCCTG CAGGCCAAGT CAGTAGCTGT CAAGACCACA 
CGATTTGCCT CATTCCCTGA CTACCTGGTC ATCCAGATCA AGAAGTTCAC CTTCGGCTTA 
GACTGGGTGC CCAAGAAACT GGATGTGTCC ATCGAGATGC CAGAGGAGCT CGACATCTCC 
CAGTTGAGGG GCACAGGGCT GCAGCCCGGA GAGGAGGAGC TGCCAGACAT TGCCCCACCC 
CTGGTCACTC CGGATGAGCC CAAAGGTAGC CTTGGTTTCT ATGGCAACGA AGACGAAGAC 
TCCTTCTGCT CCCCTCACTT CTCCTCTCCG ACATCGCCCA TGCTGGATGA ATCAGTCATC 
ATCCAGCTGG TGGAGATGGG ATTCCCTATG GACGCCTGCC GCAAAGCTGT CTACTACACG 
GGCAACAGCG GGGCTGAGGC CGCCATGAAC TGGGTCATGT CACACATGGA TGATCCAGAT 
TTTGCAAACC CCCTCATCCT GCCTGGCTCT AGTGGGCCGG GCTCCACAAG CGCAGCAGCC 
GACCCCCCTC CTGAGGACTG TGTGACCACC ATTGTCTCCA TGGGCTTCTC CCGGGACCAG 
GCCTTGAAAG CGCTGCGGGC CACGAACAAT AGTTTAGAAC GGGCTGTGGA CTGGATCTTC 
AGTCACATTG ACGACCTGGA TGCTGAAGCT GCCATGGACA TCTCAGAGGG CCGCTCAGCT 
GCCGACTCCA TCTCTGAGTC TGTGCCAGTG GGACCTAAAG TCCGGGATGG TCCTGGAAAG 
TATCAGCTCT TTGCCTTCAT TAGTCACATG GGCACCTCTA CCATGTGTGG TCACTACGTC 
TGCCACATCA AGAAAGAAGG CAGATGGGTG ATCTACAATG ACCAGAAAGT GTGTGCCTCC 
GAGAAGCCGC CCAAGGACCT GGGCTACATC TACTTCTACC AGAGAGTGGC CAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001092006.1
CDS	60..2636
Translation	MAELSEEALLSVLPTIRVPKAGDRVHKDECAFSFDTPESEGGLYICMNTFLGFGKQYVERHFNKTGQRVYLHLRRTRRPKEEDPATGTGDPPRKKPTRLAIGVEGGFDLSEEKFELDEDVKIVILPDYLEIARDGLGGLPDIVRDRVTSAVEALLSADSASRKQEVQAWDGEVRQVSKHAFSLKQLDNPARIPPCGWKCSKCDMRENLWLNLTDGSILCGRRYFDGSGGNNHAVEHYRETGYPLAVKLGTITPDGADVYSYDEDDMVLDPSLAEHLSHFGIDMLKMQKTDKTMTELEIDMNQRIGEWELIQESGVPLKPLFGPGYTGIRNLGNSCYLNSVVQVLFSIPDFQRKYVDKLEKIFQNAPTDPTQDFSTQVAKLGHGLLSGEYSKPVPESGDGERVPEQKEVQDGIAPRMFKALIGKGHPEFSTNRQQDAQEFFLHLINMVERNCRSSENPNEVFRFLVEEKIKCLATEKVKYTQRVDYIMQLPVPMDAALNKEELLEYEEKKRQAEEEKMALPELVRAQVPFSSCLEAYGAPEQVDDFWSTALQAKSVAVKTTRFASFPDYLVIQIKKFTFGLDWVPKKLDVSIEMPEELDISQLRGTGLQPGEEELPDIAPPLVTPDEPKGSLGFYGNEDEDSFCSPHFSSPTSPMLDESVIIQLVEMGFPMDACRKAVYYTGNSGAEAAMNWVMSHMDDPDFANPLILPGSSGPGSTSAAADPPPEDCVTTIVSMGFSRDQALKALRATNNSLERAVDWIFSHIDDLDAEAAMDISEGRSAADSISESVPVGPKVRDGPGKYQLFAFISHMGTSTMCGHYVCHIKKEGRWVIYNDQKVCASEKPPKDLGYIYFYQRVAS

Target ORF information:

RefSeq Version	NM_001098536.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 5 (USP5), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001098536.1

ORF Insert Sequence:

ATGGCGGAGC TGAGTGAGGA GGCGCTGCTG TCAGTATTAC CGACGATCCG GGTCCCTAAG 
GCTGGAGACC GGGTCCACAA AGACGAGTGC GCCTTCTCCT TCGACACGCC GGAGTCTGAG 
GGGGGCCTCT ACATCTGTAT GAACACGTTT CTGGGCTTTG GGAAACAGTA TGTGGAGAGA 
CATTTCAATA AGACCGGCCA GCGAGTCTAC TTGCACCTCC GGCGGACCCG GCGCCCGAAA 
GAGGAGGACC CTGCTACAGG CACTGGAGAC CCACCCCGGA AGAAGCCCAC GCGGCTGGCT 
ATTGGTGTTG AAGGCGGATT TGACCTTAGC GAGGAGAAGT TTGAATTAGA CGAGGATGTG 
AAGATTGTCA TTTTGCCAGA TTACCTGGAG ATTGCCCGGG ATGGACTGGG GGGACTGCCT 
GACATTGTCA GAGATCGGGT GACCAGTGCA GTGGAGGCCC TACTGTCGGC CGACTCAGCC 
TCCCGCAAGC AGGAGGTGCA GGCATGGGAT GGGGAAGTAC GGCAGGTGTC TAAGCATGCC 
TTCAGCCTCA AGCAGTTGGA CAACCCTGCT CGAATCCCTC CCTGTGGCTG GAAGTGCTCC 
AAGTGTGACA TGAGAGAGAA CCTGTGGCTC AACCTGACTG ATGGCTCCAT CCTCTGTGGG 
CGACGCTACT TCGATGGCAG TGGGGGCAAC AACCACGCTG TGGAGCACTA CCGAGAGACA 
GGCTACCCGT TAGCTGTCAA GCTGGGCACC ATCACCCCTG ATGGAGCTGA CGTGTACTCA 
TATGATGAGG ATGACATGGT CCTGGACCCC AGCCTGGCTG AGCACCTGTC CCACTTCGGC 
ATCGACATGC TGAAGATGCA GAAGACAGAC AAGACGATGA CTGAGTTGGA GATAGACATG 
AACCAGCGGA TTGGTGAATG GGAGCTGATC CAGGAGTCAG GTGTGCCACT CAAGCCCCTG 
TTTGGGCCTG GCTACACAGG CATCCGGAAC CTGGGTAACA GCTGCTACCT CAACTCTGTG 
GTCCAGGTGC TCTTCAGCAT CCCTGACTTC CAGAGGAAGT ATGTGGATAA GCTGGAGAAG 
ATCTTCCAGA ATGCCCCGAC GGACCCTACC CAGGATTTCA GCACCCAGGT GGCCAAGCTG 
GGCCATGGCC TTCTCTCCGG GGAGTATTCC AAGCCAGTAC CGGAGTCGGG CGATGGGGAG 
CGGGTGCCAG AACAGAAGGA AGTTCAAGAT GGCATTGCCC CTCGGATGTT CAAGGCCCTC 
ATCGGCAAGG GCCACCCTGA ATTCTCCACC AACCGGCAGC AGGATGCCCA GGAGTTCTTC 
CTTCACCTTA TCAACATGGT GGAGAGGAAT TGCCGGAGCT CTGAAAATCC TAATGAAGTG 
TTCCGCTTCT TGGTGGAGGA AAAGATCAAG TGCCTGGCCA CAGAGAAGGT GAAGTACACC 
CAGCGAGTTG ACTACATCAT GCAGCTGCCT GTGCCCATGG ATGCAGCCCT TAACAAAGAG 
GAGCTTCTGG AGTACGAGGA GAAGAAGCGG CAAGCCGAAG AGGAGAAGAT GGCACTGCCA 
GAACTGGTTC GGGCCCAGGT GCCCTTCAGC TCTTGCCTGG AGGCCTACGG GGCCCCTGAG 
CAGGTCGATG ACTTCTGGAG CACGGCCCTG CAGGCCAAGT CAGTAGCTGT CAAGACCACA 
CGATTTGCCT CATTCCCTGA CTACCTGGTC ATCCAGATCA AGAAGTTCAC CTTCGGCTTA 
GACTGGGTGC CCAAGAAACT GGATGTGTCC ATCGAGATGC CAGAGGAGCT CGACATCTCC 
CAGTTGAGGG GCACAGGGCT GCAGCCCGGA GAGGAGGAGC TGCCAGACAT TGCCCCACCC 
CTGGTCACTC CGGATGAGCC CAAAGGTAGC CTTGGTTTCT ATGGCAACGA AGACGAAGAC 
TCCTTCTGCT CCCCTCACTT CTCCTCTCCG ACATCGCCCA TGCTGGATGA ATCAGTCATC 
ATCCAGCTGG TGGAGATGGG ATTCCCTATG GACGCCTGCC GCAAAGCTGT CTACTACACG 
GGCAACAGCG GGGCTGAGGC CGCCATGAAC TGGGTCATGT CACACATGGA TGATCCAGAT 
TTTGCAAACC CCCTCATCCT GCCTGGCTCT AGTGGGCCGG GCTCCACAAG CGCAGCAGCC 
GACCCCCCTC CTGAGGACTG TGTGACCACC ATTGTCTCCA TGGGCTTCTC CCGGGACCAG 
GCCTTGAAAG CGCTGCGGGC CACGAACAAT AGTTTAGAAC GGGCTGTGGA CTGGATCTTC 
AGTCACATTG ACGACCTGGA TGCTGAAGCT GCCATGGACA TCTCAGAGGG CCGCTCAGCT 
GCCGACTCCA TCTCTGAGTC TGTGCCAGTG GGACCTAAAG TCCGGGATGG TCCTGGAAAG 
TATCAGCTCT TTGCCTTCAT TAGTCACATG GGCACCTCTA CCATGTGTGG TCACTACGTC 
TGCCACATCA AGAAAGAAGG CAGATGGGTG ATCTACAATG ACCAGAAAGT GTGTGCCTCC 
GAGAAGCCGC CCAAGGACCT GGGCTACATC TACTTCTACC AGAGAGTGGC CAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

USP5 ( NM_001098536.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001092006.1 Homo sapiens ubiquitin specific peptidase 5 (USP5), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu31063
Clone ID Related Accession (Same CDS sequence)	NM_001098536.1 , NM_001098536.2
Accession Version	NM_001098536.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2577bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-15
Organism	Homo sapiens(human)
Product	ubiquitin carboxyl-terminal hydrolase 5 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from U47927.1 and BC005139.2. On Jun 2, 2019 this sequence version replaced NM_001098536.1. Transcript Variant: This variant (1) represents the longer transcript and it encodes the longer protein (isoform 1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC005139.2, SRR1660805.55860.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END##

ATGGCGGAGC TGAGTGAGGA GGCGCTGCTG TCAGTATTAC CGACGATCCG GGTCCCTAAG 
GCTGGAGACC GGGTCCACAA AGACGAGTGC GCCTTCTCCT TCGACACGCC GGAGTCTGAG 
GGGGGCCTCT ACATCTGTAT GAACACGTTT CTGGGCTTTG GGAAACAGTA TGTGGAGAGA 
CATTTCAATA AGACCGGCCA GCGAGTCTAC TTGCACCTCC GGCGGACCCG GCGCCCGAAA 
GAGGAGGACC CTGCTACAGG CACTGGAGAC CCACCCCGGA AGAAGCCCAC GCGGCTGGCT 
ATTGGTGTTG AAGGCGGATT TGACCTTAGC GAGGAGAAGT TTGAATTAGA CGAGGATGTG 
AAGATTGTCA TTTTGCCAGA TTACCTGGAG ATTGCCCGGG ATGGACTGGG GGGACTGCCT 
GACATTGTCA GAGATCGGGT GACCAGTGCA GTGGAGGCCC TACTGTCGGC CGACTCAGCC 
TCCCGCAAGC AGGAGGTGCA GGCATGGGAT GGGGAAGTAC GGCAGGTGTC TAAGCATGCC 
TTCAGCCTCA AGCAGTTGGA CAACCCTGCT CGAATCCCTC CCTGTGGCTG GAAGTGCTCC 
AAGTGTGACA TGAGAGAGAA CCTGTGGCTC AACCTGACTG ATGGCTCCAT CCTCTGTGGG 
CGACGCTACT TCGATGGCAG TGGGGGCAAC AACCACGCTG TGGAGCACTA CCGAGAGACA 
GGCTACCCGT TAGCTGTCAA GCTGGGCACC ATCACCCCTG ATGGAGCTGA CGTGTACTCA 
TATGATGAGG ATGACATGGT CCTGGACCCC AGCCTGGCTG AGCACCTGTC CCACTTCGGC 
ATCGACATGC TGAAGATGCA GAAGACAGAC AAGACGATGA CTGAGTTGGA GATAGACATG 
AACCAGCGGA TTGGTGAATG GGAGCTGATC CAGGAGTCAG GTGTGCCACT CAAGCCCCTG 
TTTGGGCCTG GCTACACAGG CATCCGGAAC CTGGGTAACA GCTGCTACCT CAACTCTGTG 
GTCCAGGTGC TCTTCAGCAT CCCTGACTTC CAGAGGAAGT ATGTGGATAA GCTGGAGAAG 
ATCTTCCAGA ATGCCCCGAC GGACCCTACC CAGGATTTCA GCACCCAGGT GGCCAAGCTG 
GGCCATGGCC TTCTCTCCGG GGAGTATTCC AAGCCAGTAC CGGAGTCGGG CGATGGGGAG 
CGGGTGCCAG AACAGAAGGA AGTTCAAGAT GGCATTGCCC CTCGGATGTT CAAGGCCCTC 
ATCGGCAAGG GCCACCCTGA ATTCTCCACC AACCGGCAGC AGGATGCCCA GGAGTTCTTC 
CTTCACCTTA TCAACATGGT GGAGAGGAAT TGCCGGAGCT CTGAAAATCC TAATGAAGTG 
TTCCGCTTCT TGGTGGAGGA AAAGATCAAG TGCCTGGCCA CAGAGAAGGT GAAGTACACC 
CAGCGAGTTG ACTACATCAT GCAGCTGCCT GTGCCCATGG ATGCAGCCCT TAACAAAGAG 
GAGCTTCTGG AGTACGAGGA GAAGAAGCGG CAAGCCGAAG AGGAGAAGAT GGCACTGCCA 
GAACTGGTTC GGGCCCAGGT GCCCTTCAGC TCTTGCCTGG AGGCCTACGG GGCCCCTGAG 
CAGGTCGATG ACTTCTGGAG CACGGCCCTG CAGGCCAAGT CAGTAGCTGT CAAGACCACA 
CGATTTGCCT CATTCCCTGA CTACCTGGTC ATCCAGATCA AGAAGTTCAC CTTCGGCTTA 
GACTGGGTGC CCAAGAAACT GGATGTGTCC ATCGAGATGC CAGAGGAGCT CGACATCTCC 
CAGTTGAGGG GCACAGGGCT GCAGCCCGGA GAGGAGGAGC TGCCAGACAT TGCCCCACCC 
CTGGTCACTC CGGATGAGCC CAAAGGTAGC CTTGGTTTCT ATGGCAACGA AGACGAAGAC 
TCCTTCTGCT CCCCTCACTT CTCCTCTCCG ACATCGCCCA TGCTGGATGA ATCAGTCATC 
ATCCAGCTGG TGGAGATGGG ATTCCCTATG GACGCCTGCC GCAAAGCTGT CTACTACACG 
GGCAACAGCG GGGCTGAGGC CGCCATGAAC TGGGTCATGT CACACATGGA TGATCCAGAT 
TTTGCAAACC CCCTCATCCT GCCTGGCTCT AGTGGGCCGG GCTCCACAAG CGCAGCAGCC 
GACCCCCCTC CTGAGGACTG TGTGACCACC ATTGTCTCCA TGGGCTTCTC CCGGGACCAG 
GCCTTGAAAG CGCTGCGGGC CACGAACAAT AGTTTAGAAC GGGCTGTGGA CTGGATCTTC 
AGTCACATTG ACGACCTGGA TGCTGAAGCT GCCATGGACA TCTCAGAGGG CCGCTCAGCT 
GCCGACTCCA TCTCTGAGTC TGTGCCAGTG GGACCTAAAG TCCGGGATGG TCCTGGAAAG 
TATCAGCTCT TTGCCTTCAT TAGTCACATG GGCACCTCTA CCATGTGTGG TCACTACGTC 
TGCCACATCA AGAAAGAAGG CAGATGGGTG ATCTACAATG ACCAGAAAGT GTGTGCCTCC 
GAGAAGCCGC CCAAGGACCT GGGCTACATC TACTTCTACC AGAGAGTGGC CAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001092006.1
CDS	31..2607
Translation	MAELSEEALLSVLPTIRVPKAGDRVHKDECAFSFDTPESEGGLYICMNTFLGFGKQYVERHFNKTGQRVYLHLRRTRRPKEEDPATGTGDPPRKKPTRLAIGVEGGFDLSEEKFELDEDVKIVILPDYLEIARDGLGGLPDIVRDRVTSAVEALLSADSASRKQEVQAWDGEVRQVSKHAFSLKQLDNPARIPPCGWKCSKCDMRENLWLNLTDGSILCGRRYFDGSGGNNHAVEHYRETGYPLAVKLGTITPDGADVYSYDEDDMVLDPSLAEHLSHFGIDMLKMQKTDKTMTELEIDMNQRIGEWELIQESGVPLKPLFGPGYTGIRNLGNSCYLNSVVQVLFSIPDFQRKYVDKLEKIFQNAPTDPTQDFSTQVAKLGHGLLSGEYSKPVPESGDGERVPEQKEVQDGIAPRMFKALIGKGHPEFSTNRQQDAQEFFLHLINMVERNCRSSENPNEVFRFLVEEKIKCLATEKVKYTQRVDYIMQLPVPMDAALNKEELLEYEEKKRQAEEEKMALPELVRAQVPFSSCLEAYGAPEQVDDFWSTALQAKSVAVKTTRFASFPDYLVIQIKKFTFGLDWVPKKLDVSIEMPEELDISQLRGTGLQPGEEELPDIAPPLVTPDEPKGSLGFYGNEDEDSFCSPHFSSPTSPMLDESVIIQLVEMGFPMDACRKAVYYTGNSGAEAAMNWVMSHMDDPDFANPLILPGSSGPGSTSAAADPPPEDCVTTIVSMGFSRDQALKALRATNNSLERAVDWIFSHIDDLDAEAAMDISEGRSAADSISESVPVGPKVRDGPGKYQLFAFISHMGTSTMCGHYVCHIKKEGRWVIYNDQKVCASEKPPKDLGYIYFYQRVAS

Target ORF information:

RefSeq Version	NM_001098536.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin specific peptidase 5 (USP5), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001098536.2

ORF Insert Sequence:

ATGGCGGAGC TGAGTGAGGA GGCGCTGCTG TCAGTATTAC CGACGATCCG GGTCCCTAAG 
GCTGGAGACC GGGTCCACAA AGACGAGTGC GCCTTCTCCT TCGACACGCC GGAGTCTGAG 
GGGGGCCTCT ACATCTGTAT GAACACGTTT CTGGGCTTTG GGAAACAGTA TGTGGAGAGA 
CATTTCAATA AGACCGGCCA GCGAGTCTAC TTGCACCTCC GGCGGACCCG GCGCCCGAAA 
GAGGAGGACC CTGCTACAGG CACTGGAGAC CCACCCCGGA AGAAGCCCAC GCGGCTGGCT 
ATTGGTGTTG AAGGCGGATT TGACCTTAGC GAGGAGAAGT TTGAATTAGA CGAGGATGTG 
AAGATTGTCA TTTTGCCAGA TTACCTGGAG ATTGCCCGGG ATGGACTGGG GGGACTGCCT 
GACATTGTCA GAGATCGGGT GACCAGTGCA GTGGAGGCCC TACTGTCGGC CGACTCAGCC 
TCCCGCAAGC AGGAGGTGCA GGCATGGGAT GGGGAAGTAC GGCAGGTGTC TAAGCATGCC 
TTCAGCCTCA AGCAGTTGGA CAACCCTGCT CGAATCCCTC CCTGTGGCTG GAAGTGCTCC 
AAGTGTGACA TGAGAGAGAA CCTGTGGCTC AACCTGACTG ATGGCTCCAT CCTCTGTGGG 
CGACGCTACT TCGATGGCAG TGGGGGCAAC AACCACGCTG TGGAGCACTA CCGAGAGACA 
GGCTACCCGT TAGCTGTCAA GCTGGGCACC ATCACCCCTG ATGGAGCTGA CGTGTACTCA 
TATGATGAGG ATGACATGGT CCTGGACCCC AGCCTGGCTG AGCACCTGTC CCACTTCGGC 
ATCGACATGC TGAAGATGCA GAAGACAGAC AAGACGATGA CTGAGTTGGA GATAGACATG 
AACCAGCGGA TTGGTGAATG GGAGCTGATC CAGGAGTCAG GTGTGCCACT CAAGCCCCTG 
TTTGGGCCTG GCTACACAGG CATCCGGAAC CTGGGTAACA GCTGCTACCT CAACTCTGTG 
GTCCAGGTGC TCTTCAGCAT CCCTGACTTC CAGAGGAAGT ATGTGGATAA GCTGGAGAAG 
ATCTTCCAGA ATGCCCCGAC GGACCCTACC CAGGATTTCA GCACCCAGGT GGCCAAGCTG 
GGCCATGGCC TTCTCTCCGG GGAGTATTCC AAGCCAGTAC CGGAGTCGGG CGATGGGGAG 
CGGGTGCCAG AACAGAAGGA AGTTCAAGAT GGCATTGCCC CTCGGATGTT CAAGGCCCTC 
ATCGGCAAGG GCCACCCTGA ATTCTCCACC AACCGGCAGC AGGATGCCCA GGAGTTCTTC 
CTTCACCTTA TCAACATGGT GGAGAGGAAT TGCCGGAGCT CTGAAAATCC TAATGAAGTG 
TTCCGCTTCT TGGTGGAGGA AAAGATCAAG TGCCTGGCCA CAGAGAAGGT GAAGTACACC 
CAGCGAGTTG ACTACATCAT GCAGCTGCCT GTGCCCATGG ATGCAGCCCT TAACAAAGAG 
GAGCTTCTGG AGTACGAGGA GAAGAAGCGG CAAGCCGAAG AGGAGAAGAT GGCACTGCCA 
GAACTGGTTC GGGCCCAGGT GCCCTTCAGC TCTTGCCTGG AGGCCTACGG GGCCCCTGAG 
CAGGTCGATG ACTTCTGGAG CACGGCCCTG CAGGCCAAGT CAGTAGCTGT CAAGACCACA 
CGATTTGCCT CATTCCCTGA CTACCTGGTC ATCCAGATCA AGAAGTTCAC CTTCGGCTTA 
GACTGGGTGC CCAAGAAACT GGATGTGTCC ATCGAGATGC CAGAGGAGCT CGACATCTCC 
CAGTTGAGGG GCACAGGGCT GCAGCCCGGA GAGGAGGAGC TGCCAGACAT TGCCCCACCC 
CTGGTCACTC CGGATGAGCC CAAAGGTAGC CTTGGTTTCT ATGGCAACGA AGACGAAGAC 
TCCTTCTGCT CCCCTCACTT CTCCTCTCCG ACATCGCCCA TGCTGGATGA ATCAGTCATC 
ATCCAGCTGG TGGAGATGGG ATTCCCTATG GACGCCTGCC GCAAAGCTGT CTACTACACG 
GGCAACAGCG GGGCTGAGGC CGCCATGAAC TGGGTCATGT CACACATGGA TGATCCAGAT 
TTTGCAAACC CCCTCATCCT GCCTGGCTCT AGTGGGCCGG GCTCCACAAG CGCAGCAGCC 
GACCCCCCTC CTGAGGACTG TGTGACCACC ATTGTCTCCA TGGGCTTCTC CCGGGACCAG 
GCCTTGAAAG CGCTGCGGGC CACGAACAAT AGTTTAGAAC GGGCTGTGGA CTGGATCTTC 
AGTCACATTG ACGACCTGGA TGCTGAAGCT GCCATGGACA TCTCAGAGGG CCGCTCAGCT 
GCCGACTCCA TCTCTGAGTC TGTGCCAGTG GGACCTAAAG TCCGGGATGG TCCTGGAAAG 
TATCAGCTCT TTGCCTTCAT TAGTCACATG GGCACCTCTA CCATGTGTGG TCACTACGTC 
TGCCACATCA AGAAAGAAGG CAGATGGGTG ATCTACAATG ACCAGAAAGT GTGTGCCTCC 
GAGAAGCCGC CCAAGGACCT GGGCTACATC TACTTCTACC AGAGAGTGGC CAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

USP5 promotes tumorigenesis and progression of pancreatic cancer by stabilizing FoxM1 protein.
Biochemical and biophysical research communications492(1)48-54(2017 10)
Li XY,Wu HY,Mao XF,Jiang LX,Wang YX

Inhibition of the deubiquitinase USP5 leads to c-Maf protein degradation and myeloma cell apoptosis.
Cell death & disease8(9)e3058(2017 Sep)
Wang S,Juan J,Zhang Z,Du Y,Xu Y,Tong J,Cao B,Moran MF,Zeng Y,Mao X

Smurf1 represses TNF-? production through ubiquitination and destabilization of USP5.
Biochemical and biophysical research communications474(3)491-496(2016 06)
Qian G,Ren Y,Zuo Y,Yuan Y,Zhao P,Wang X,Cheng Q,Liu J,Zhang L,Guo T,Liu C,Zheng H

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USP5 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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