tpo cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	tpo
Entrez Gene ID	100036738
Full Name	thyroid peroxidase
Synonyms	msa,tpx,xtpo
General protein information	Preferred Names thyroid peroxidase Names thyroid peroxidase
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_002936302.2	XP_002936348.2	thyroid peroxidase
XM_031902754.1	XP_031758614.1	thyroid peroxidase

Pathways from BioSystems

Homologies

Homo sapiens (human)	TPO	NP_000538.3
Rattus norvegicus (Norway rat)	Tpo	NP_062226.2
Xenopus tropicalis (tropical clawed frog)	tpo	XP_002936348.2
Gallus gallus (chicken)	TPO	XP_001235673.2
Pan troglodytes (chimpanzee)	TPO	XP_003308907.1
Canis lupus familiaris (dog)	TPO	NP_001003009.2
Mus musculus (house mouse)	Tpo	NP_033443.1
Danio rerio (zebrafish)	LOC100331172	XP_003201429.2
	TPO	XP_001117795.1
Bos taurus (cattle)	TPO	XP_002683990.1

Gene Ontology

The following tpo gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the tpo cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OXa11049	XM_002936302.2 Latest version!	Xenopus tropicalis thyroid peroxidase (tpo), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OXa59079	XM_031902754.1 Latest version!	Xenopus tropicalis thyroid peroxidase (tpo), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

tpo ( XM_002936302.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002936348.2 Xenopus tropicalis thyroid peroxidase (tpo), mRNA.

Price & Availability↑

CloneID	OXa11049
Clone ID Related Accession (Same CDS sequence)	XM_002936302.2
Accession Version	XM_002936302.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2589bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	thyroid peroxidase
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jun 18, 2013 this sequence version replaced XM_002936302.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 14% of CDS bases ##RefSeq-Attributes-END##

ATGGGAAGGC TTACACTGCA CAAAAGCGCC TGGGACATTG CTGTTCTCAT TGTACAAGAG 
TTCCGGAATA GACGAAGAAA CCACATTTAC AAAATCACCC AAGAAAGAAA TTCATCTCAA 
ACAGACAAGA CTTCTTTAGA ACAGCACCCA GAAAGTGAAG ATGTATCCCA TACAGGGTCC 
TCGTTTGAAG CAGTGCTTGC CAACCTAACT GGGTGTGTCC CACAAACACT TTCTCCATTC 
TGTCCCAACA ATTGCTTGGC CGGGAAATAC AGACACATTA CTGGTGCTTG CAACAACAGA 
AACAATCCGG CCTGGGGCGC TGCAAATACT CTTCTGGCTC GTTGGCTTCC TGCACAATAT 
GAAGATGGAA TTGGTGAACC GAAGGGATGG AGAGAAGAGT TTTTATACAA TGGGTTTCGT 
CTCCCTCAGG TTCGTGAGGT GACTAATAAG ATAATTCATG CATCGAGCAA GGCTTTCCGG 
GAAGATGACT TATATTCCCA CATCATAATC GAGTGGGGGC AATACATTGA CCATGACATT 
GCTTTTACAC CACAGAGTGT AAGTAAGACA CCGGCAATAG GAAGTGTGGA TTGCAAGTCT 
ACCTGTGAGA ATCTCTACCC ATGTTATCCT ATAAAGCTTT CTGAGGAGGA CCCTCTATCA 
CAAGGTGGTT GTATTCCATT TTATCGATCA TCTTCTGCCT GTGGCACAGG GCGTGGGGGT 
TTAAAGTTTG GAAACCCCTC ATTATTAAAC CCAAGAGAGC AAATAAATGG ATTAACATCA 
TTTATTGATG CATCAACTGT TTATGGAAGC TCAGAATCAT TACAACACAA GCTGAAAAAC 
CTTTCTAGTG AGGAAGGCCT CTTGAGAGTA AATGTAAAAT ATTCTGATTC CAGTCGAGAG 
TATCTGCCTT TTGAGACACC ATCAGCATGT ATGCAGGACC CTACAGCAGA TAATGCTGAG 
AGAATAGACT GTTTCTTTGC AGGTGAAGGC AGGGCCAATG AAGTTATAAC CCTGGCAGCA 
GTTCACACAT TATGGTTAAG AGAGCACAAC CGCATAGCCA AAGCCTTGAA AAAACTAAAT 
CCTCACTGGA ATTCAGAAAC CACTTATCAG GAGGCTCGGA AAATTGTTGG TGCCCTTCAT 
CAGATAATTA CTTTTCGAGA TTACATGCCA AAAATTCTGG GTAAAGCCGC ATATGATCAG 
TATATTGGTC TGTACAAAGG ATACAATCAA AAAACAAATC CCTCTATATC AAATATATTT 
ACAACGGCGG CATTTCGTTT TGGGCATGCA ACTATTCCAC CGATGGTGCA CAGACTCAAT 
TCTCAGTATG TAGATGATCC AAAATATCCC AGTTTGCCCC TGAATGAAGT TTTCTTTCGC 
CCATGGAGGA TTGTAAAAGA AGGAGGCTTG GATCCATTGA TTAGAGGACT TCTTGGAAAA 
TCAGCAAAAC TGCAAACACA AGTAGACATG ATCAATGAGG AATTAACAGA AAAACTGCTT 
GTAATGACCA ATAATGGTTC GATGGACTTA GCTTCCTTGA ATCTACAAAG AGGTCGAGAT 
CATGGCCTAC CAGGCTACAA TGACTGGAGG GAATTTTGTG GTTTATCAAG ACTGGCGACT 
CCAGCAGATC TAATAAATGC AGTATCTGAT CAGAAATTAG TAGCCAAAAT GATTGCTTTG 
TATAGTCATC CCGACAACAT TGACGTGTGG CTCGGGGGTC TAGCAGAGGA CTTCCTTCCA 
GGAGCTAGAA CTGGACCTCT TTTTGCATGT TTGATTGGAA AGCAAATGCA GGCACTGCGG 
GAAGGAGACA GGTTTTGGTA TGAAAACAAC AACATTTTTA CGAAAATTCA GAGATCTGAA 
CTAGAAAAAC ATTCTCTTTC GCGTGTAATT TGTGATAATA CAGGTTTATC CCATGTTCCC 
CTCGATGCCT TTCTACTGGG AAATTATCCA GACAACTTTG TGTCATGTGA CAGTATCCCT 
GGCATAAACC TGGAAGCCTG GAAAGAATCT CCTGAAAAAG GCACAACCTG TGGATCCCCA 
AGAAAAATAG AAAATGGAGA TTTTGCATTT TGTTCAGAGG CTACTGTAAT TTATTCTTGC 
CACAGCGGGT ATCGCTTAGA AGGCCATGAA GAAATAACCT GTCAAGGAAA TGAATGGAGT 
AACCAACCAC CCATTTGTTC AGATATCAAT GAATGTGAAG ATCAGCCTAA TGGCCCATGC 
CATTCTTCTG CTAAATGCAA GAACACATTG GGCGGCTTTC ATTGCTTATG CACAGATCCA 
TATGAACTTG ACAAGGATAA AAGGACATGC GTGGATTCAG GAAGACTCCC TAGAGGATCT 
TTAGTGTCCA TCATTTTAGT TGCTGTCTTG TTTGTGTCCT GGGCCGGAAT GTGCTGGATT 
CTAATTTGCA GAGGAGCATC CATATTTTGG TCATTGACTA AAGCTCACAA TGTGGAAGGT 
GGGAAGAAGC CATCGGTAGT GATGATTTAC TCCAGGTGTG AAAGTAAAAC CATGCCATTA 
CCATTGATTC CATCTGAACA AGTGGCCAAG GATGTAAGCA ATGGACACCA AATTCTACTT 
CATGTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002936348.2
CDS	1..2589
Translation	MGRLTLHKSAWDIAVLIVQEFRNRRRNHIYKITQERNSSQTDKTSLEQHPESEDVSHTGSSFEAVLANLTGCVPQTLSPFCPNNCLAGKYRHITGACNNRNNPAWGAANTLLARWLPAQYEDGIGEPKGWREEFLYNGFRLPQVREVTNKIIHASSKAFREDDLYSHIIIEWGQYIDHDIAFTPQSVSKTPAIGSVDCKSTCENLYPCYPIKLSEEDPLSQGGCIPFYRSSSACGTGRGGLKFGNPSLLNPREQINGLTSFIDASTVYGSSESLQHKLKNLSSEEGLLRVNVKYSDSSREYLPFETPSACMQDPTADNAERIDCFFAGEGRANEVITLAAVHTLWLREHNRIAKALKKLNPHWNSETTYQEARKIVGALHQIITFRDYMPKILGKAAYDQYIGLYKGYNQKTNPSISNIFTTAAFRFGHATIPPMVHRLNSQYVDDPKYPSLPLNEVFFRPWRIVKEGGLDPLIRGLLGKSAKLQTQVDMINEELTEKLLVMTNNGSMDLASLNLQRGRDHGLPGYNDWREFCGLSRLATPADLINAVSDQKLVAKMIALYSHPDNIDVWLGGLAEDFLPGARTGPLFACLIGKQMQALREGDRFWYENNNIFTKIQRSELEKHSLSRVICDNTGLSHVPLDAFLLGNYPDNFVSCDSIPGINLEAWKESPEKGTTCGSPRKIENGDFAFCSEATVIYSCHSGYRLEGHEEITCQGNEWSNQPPICSDINECEDQPNGPCHSSAKCKNTLGGFHCLCTDPYELDKDKRTCVDSGRLPRGSLVSIILVAVLFVSWAGMCWILICRGASIFWSLTKAHNVEGGKKPSVVMIYSRCESKTMPLPLIPSEQVAKDVSNGHQILLHV

Target ORF information:

RefSeq Version	XM_002936302.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis thyroid peroxidase (tpo), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002936302.2

ORF Insert Sequence:

ATGGGAAGGC TTACACTGCA CAAAAGCGCC TGGGACATTG CTGTTCTCAT TGTACAAGAG 
TTCCGGAATA GACGAAGAAA CCACATTTAC AAAATCACCC AAGAAAGAAA TTCATCTCAA 
ACAGACAAGA CTTCTTTAGA ACAGCACCCA GAAAGTGAAG ATGTATCCCA TACAGGGTCC 
TCGTTTGAAG CAGTGCTTGC CAACCTAACT GGGTGTGTCC CACAAACACT TTCTCCATTC 
TGTCCCAACA ATTGCTTGGC CGGGAAATAC AGACACATTA CTGGTGCTTG CAACAACAGA 
AACAATCCGG CCTGGGGCGC TGCAAATACT CTTCTGGCTC GTTGGCTTCC TGCACAATAT 
GAAGATGGAA TTGGTGAACC GAAGGGATGG AGAGAAGAGT TTTTATACAA TGGGTTTCGT 
CTCCCTCAGG TTCGTGAGGT GACTAATAAG ATAATTCATG CATCGAGCAA GGCTTTCCGG 
GAAGATGACT TATATTCCCA CATCATAATC GAGTGGGGGC AATACATTGA CCATGACATT 
GCTTTTACAC CACAGAGTGT AAGTAAGACA CCGGCAATAG GAAGTGTGGA TTGCAAGTCT 
ACCTGTGAGA ATCTCTACCC ATGTTATCCT ATAAAGCTTT CTGAGGAGGA CCCTCTATCA 
CAAGGTGGTT GTATTCCATT TTATCGATCA TCTTCTGCCT GTGGCACAGG GCGTGGGGGT 
TTAAAGTTTG GAAACCCCTC ATTATTAAAC CCAAGAGAGC AAATAAATGG ATTAACATCA 
TTTATTGATG CATCAACTGT TTATGGAAGC TCAGAATCAT TACAACACAA GCTGAAAAAC 
CTTTCTAGTG AGGAAGGCCT CTTGAGAGTA AATGTAAAAT ATTCTGATTC CAGTCGAGAG 
TATCTGCCTT TTGAGACACC ATCAGCATGT ATGCAGGACC CTACAGCAGA TAATGCTGAG 
AGAATAGACT GTTTCTTTGC AGGTGAAGGC AGGGCCAATG AAGTTATAAC CCTGGCAGCA 
GTTCACACAT TATGGTTAAG AGAGCACAAC CGCATAGCCA AAGCCTTGAA AAAACTAAAT 
CCTCACTGGA ATTCAGAAAC CACTTATCAG GAGGCTCGGA AAATTGTTGG TGCCCTTCAT 
CAGATAATTA CTTTTCGAGA TTACATGCCA AAAATTCTGG GTAAAGCCGC ATATGATCAG 
TATATTGGTC TGTACAAAGG ATACAATCAA AAAACAAATC CCTCTATATC AAATATATTT 
ACAACGGCGG CATTTCGTTT TGGGCATGCA ACTATTCCAC CGATGGTGCA CAGACTCAAT 
TCTCAGTATG TAGATGATCC AAAATATCCC AGTTTGCCCC TGAATGAAGT TTTCTTTCGC 
CCATGGAGGA TTGTAAAAGA AGGAGGCTTG GATCCATTGA TTAGAGGACT TCTTGGAAAA 
TCAGCAAAAC TGCAAACACA AGTAGACATG ATCAATGAGG AATTAACAGA AAAACTGCTT 
GTAATGACCA ATAATGGTTC GATGGACTTA GCTTCCTTGA ATCTACAAAG AGGTCGAGAT 
CATGGCCTAC CAGGCTACAA TGACTGGAGG GAATTTTGTG GTTTATCAAG ACTGGCGACT 
CCAGCAGATC TAATAAATGC AGTATCTGAT CAGAAATTAG TAGCCAAAAT GATTGCTTTG 
TATAGTCATC CCGACAACAT TGACGTGTGG CTCGGGGGTC TAGCAGAGGA CTTCCTTCCA 
GGAGCTAGAA CTGGACCTCT TTTTGCATGT TTGATTGGAA AGCAAATGCA GGCACTGCGG 
GAAGGAGACA GGTTTTGGTA TGAAAACAAC AACATTTTTA CGAAAATTCA GAGATCTGAA 
CTAGAAAAAC ATTCTCTTTC GCGTGTAATT TGTGATAATA CAGGTTTATC CCATGTTCCC 
CTCGATGCCT TTCTACTGGG AAATTATCCA GACAACTTTG TGTCATGTGA CAGTATCCCT 
GGCATAAACC TGGAAGCCTG GAAAGAATCT CCTGAAAAAG GCACAACCTG TGGATCCCCA 
AGAAAAATAG AAAATGGAGA TTTTGCATTT TGTTCAGAGG CTACTGTAAT TTATTCTTGC 
CACAGCGGGT ATCGCTTAGA AGGCCATGAA GAAATAACCT GTCAAGGAAA TGAATGGAGT 
AACCAACCAC CCATTTGTTC AGATATCAAT GAATGTGAAG ATCAGCCTAA TGGCCCATGC 
CATTCTTCTG CTAAATGCAA GAACACATTG GGCGGCTTTC ATTGCTTATG CACAGATCCA 
TATGAACTTG ACAAGGATAA AAGGACATGC GTGGATTCAG GAAGACTCCC TAGAGGATCT 
TTAGTGTCCA TCATTTTAGT TGCTGTCTTG TTTGTGTCCT GGGCCGGAAT GTGCTGGATT 
CTAATTTGCA GAGGAGCATC CATATTTTGG TCATTGACTA AAGCTCACAA TGTGGAAGGT 
GGGAAGAAGC CATCGGTAGT GATGATTTAC TCCAGGTGTG AAAGTAAAAC CATGCCATTA 
CCATTGATTC CATCTGAACA AGTGGCCAAG GATGTAAGCA ATGGACACCA AATTCTACTT 
CATGTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tpo ( XM_031902754.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031758614.1 Xenopus tropicalis thyroid peroxidase (tpo), mRNA.

Price & Availability↑

CloneID	OXa59079
Clone ID Related Accession (Same CDS sequence)	XM_031902754.1
Accession Version	XM_031902754.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2619bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	thyroid peroxidase
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 8% of CDS bases ##RefSeq-Attributes-END##

ATGGCAAGTA TTGTGTTTTC GTATACTGTG AAATCTAACG GCACCTCCCA AGTGAAAGAA 
GTTTGCGTGT TTGAGAACAA CACCGATCAA AACAGTGTTT TTCATCTGGA CAATATACCC 
CAAAGCGCAC TACCAAGAAA TTCATCTCAA ACAGACAAGA CTTCTTTAGA ACAGCACCCA 
GAAAGTGAAG ATGTATCCCA TACAGGGTCC TCGTTTGAAG CAGTGCTTGC CAACCTAACT 
GGGTGTGTCC CACAAACACT TTCTCCATTC TGTCCCAACA ATTGCTTGGC CGGGAAATAC 
AGACACATTA CTGGTGCTTG CAACAACAGA AACAATCCGG CCTGGGGCGC TGCAAATACT 
CTTCTGGCTC GTTGGCTTCC TGCACAATAT GAAGATGGAA TTGGTGAACC GAAGGGATGG 
AGAGAAGAGT TTTTATACAA TGGGTTTCGT CTCCCTCAGG TTCGTGAGGT GACTAATAAG 
ATAATTCATG CATCGAGCAA GGCTTTCCGG GAAGATGACT TATATTCCCA CATCATAATC 
GAGTGGGGGC AATACATTGA CCATGACATT GCTTTTACAC CACAGAGTGT AAGTAAGACA 
CCGGCAATAG GAAGTGTGGA TTGCAAGTCT ACCTGTGAGA ATCTCTACCC ATGTTATCCT 
ATAAAGCTTT CTGAGGAGGA CCCTCTATCA CAAGGTGGTT GTATTCCATT TTATCGATCA 
TCTTCTGCCT GTGGCACAGG GCGTGGGGGT TTAAAGTTTG GAAACCCCTC ATTATTAAAC 
CCAAGAGAGC AAATAAATGG ATTAACATCA TTTATTGATG CATCAACTGT TTATGGAAGC 
TCAGAATCAT TACAACACAA GCTGAAAAAC CTTTCTAGTG AGGAAGGCCT CTTGAGAGTA 
AATGTAAAAT ATTCTGATTC CAGTCGAGAG TATCTGCCTT TTGAGACACC ATCAGCATGT 
ATGCAGGACC CTACAGCAGA TAATGCTGAG AGAATAGACT GTTTCTTTGC AGGTGAAGGC 
AGGGCCAATG AAGTTATAAC CCTGGCAGCA GTTCACACAT TATGGTTAAG AGAGCACAAC 
CGCATAGCCA AAGCCTTGAA AAAACTAAAT CCTCACTGGA ATTCAGAAAC CACTTATCAG 
GAGGCTCGGA AAATTGTTGG TGCCCTTCAT CAGATAATTA CTTTTCGAGA TTACATGCCA 
AAAATTCTGG GTAAAGCCGC ATATGATCAG TATATTGGTC TGTACAAAGG ATACAATCAA 
AAAACAAATC CCTCTATATC AAATATATTT ACAACGGCGG CATTTCGTTT TGGGCATGCA 
ACTATTCCAC CGATGGTGCA CAGACTCAAT TCTCAGTATG TAGATGATCC AAAATATCCC 
AGTTTGCCCC TGAATGAAGT TTTCTTTCGC CCATGGAGGA TTGTAAAAGA AGGAGGCTTG 
GATCCATTGA TTAGAGGACT TCTTGGAAAA TCAGCAAAAC TGCAAACACA AGTAGACATG 
ATCAATGAGG AATTAACAGA AAAACTGCTT GTAATGACCA ATAATGGTTC GATGGACTTA 
GCTTCCTTGA ATCTACAAAG AGGTCGAGAT CATGGCCTAC CAGGCTACAA TGACTGGAGG 
GAATTTTGTG GTTTATCAAG ACTGGCGACT CCAGCAGATC TAATAAATGC AGTATCTGAT 
CAGAAATTAG TAGCCAAAAT GATTGCTTTG TATAGTCATC CCGACAACAT TGACGTGTGG 
CTCGGGGGTC TAGCAGAGGA CTTCCTTCCA GGAGCTAGAA CTGGACCTCT TTTTGCATGT 
TTGATTGGAA AGCAAATGCA GGCACTGCGG GAAGGAGACA GGTTTTGGTA TGAAAACAAC 
AACATTTTTA CGAAAATTCA GAGATCTGAA CTAGAAAAAC ATTCTCTTTC GCGTGTAATT 
TGTGATAATA CAGGTTTATC CCATGTTCCC CTCGATGCCT TTCTACTGGG AAATTATCCA 
GACAACTTTG TGTCATGTGA CAGTATCCCT GGCATAAACC TGGAAGCCTG GAAAGAATCT 
CCTGAAAAAG GCACAACCTG TGGATCCCCA AGAAAAATAG AAAATGGAGA TTTTGCATTT 
TGTTCAGAGG CTACTGTAAT TTATTCTTGC CACAGCGGGT ATCGCTTAGA AGGCCATGAA 
GAAATAACCT GTCAAGGAAA TGAATGGAGT AACCAACCAC CCATTTGTTC AGATATCAAT 
GAATGTGAAG ATCAGCCTAA TGGCCCATGC CATTCTTCTG CTAAATGCAA GAACACATTG 
GGCGGCTTTC ATTGCTTATG CACAGATCCA TATGAACTTG ACAAGGATAA AAGGACATGC 
GTGGATTCAG GAAGACTCCC TAGAGGATCT TTAGTGTCCA TCATTTTAGT TGCTGTCTTG 
TTTGTGTCCT GGGCCGGAAT GTGCTGGATT CTAATTTGCA GAGGAGCATC CATATTTTGG 
TCATTGACTA AAGCTCACAA TGTGGAAGGT GGGAAGAAGC CATCGGTAGT GATGATTTAC 
TCCAGGTGTG AAAGTAAAAC CATGCCATTA CCATTGATTC CATCTGAACA AGTGGCCAAG 
GATGTAAGCA ATGGACACCA AATTCTACTT CATGTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031758614.1
CDS	1..2619
Translation	MASIVFSYTVKSNGTSQVKEVCVFENNTDQNSVFHLDNIPQSALPRNSSQTDKTSLEQHPESEDVSHTGSSFEAVLANLTGCVPQTLSPFCPNNCLAGKYRHITGACNNRNNPAWGAANTLLARWLPAQYEDGIGEPKGWREEFLYNGFRLPQVREVTNKIIHASSKAFREDDLYSHIIIEWGQYIDHDIAFTPQSVSKTPAIGSVDCKSTCENLYPCYPIKLSEEDPLSQGGCIPFYRSSSACGTGRGGLKFGNPSLLNPREQINGLTSFIDASTVYGSSESLQHKLKNLSSEEGLLRVNVKYSDSSREYLPFETPSACMQDPTADNAERIDCFFAGEGRANEVITLAAVHTLWLREHNRIAKALKKLNPHWNSETTYQEARKIVGALHQIITFRDYMPKILGKAAYDQYIGLYKGYNQKTNPSISNIFTTAAFRFGHATIPPMVHRLNSQYVDDPKYPSLPLNEVFFRPWRIVKEGGLDPLIRGLLGKSAKLQTQVDMINEELTEKLLVMTNNGSMDLASLNLQRGRDHGLPGYNDWREFCGLSRLATPADLINAVSDQKLVAKMIALYSHPDNIDVWLGGLAEDFLPGARTGPLFACLIGKQMQALREGDRFWYENNNIFTKIQRSELEKHSLSRVICDNTGLSHVPLDAFLLGNYPDNFVSCDSIPGINLEAWKESPEKGTTCGSPRKIENGDFAFCSEATVIYSCHSGYRLEGHEEITCQGNEWSNQPPICSDINECEDQPNGPCHSSAKCKNTLGGFHCLCTDPYELDKDKRTCVDSGRLPRGSLVSIILVAVLFVSWAGMCWILICRGASIFWSLTKAHNVEGGKKPSVVMIYSRCESKTMPLPLIPSEQVAKDVSNGHQILLHV

Target ORF information:

RefSeq Version	XM_031902754.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis thyroid peroxidase (tpo), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031902754.1

ORF Insert Sequence:

ATGGCAAGTA TTGTGTTTTC GTATACTGTG AAATCTAACG GCACCTCCCA AGTGAAAGAA 
GTTTGCGTGT TTGAGAACAA CACCGATCAA AACAGTGTTT TTCATCTGGA CAATATACCC 
CAAAGCGCAC TACCAAGAAA TTCATCTCAA ACAGACAAGA CTTCTTTAGA ACAGCACCCA 
GAAAGTGAAG ATGTATCCCA TACAGGGTCC TCGTTTGAAG CAGTGCTTGC CAACCTAACT 
GGGTGTGTCC CACAAACACT TTCTCCATTC TGTCCCAACA ATTGCTTGGC CGGGAAATAC 
AGACACATTA CTGGTGCTTG CAACAACAGA AACAATCCGG CCTGGGGCGC TGCAAATACT 
CTTCTGGCTC GTTGGCTTCC TGCACAATAT GAAGATGGAA TTGGTGAACC GAAGGGATGG 
AGAGAAGAGT TTTTATACAA TGGGTTTCGT CTCCCTCAGG TTCGTGAGGT GACTAATAAG 
ATAATTCATG CATCGAGCAA GGCTTTCCGG GAAGATGACT TATATTCCCA CATCATAATC 
GAGTGGGGGC AATACATTGA CCATGACATT GCTTTTACAC CACAGAGTGT AAGTAAGACA 
CCGGCAATAG GAAGTGTGGA TTGCAAGTCT ACCTGTGAGA ATCTCTACCC ATGTTATCCT 
ATAAAGCTTT CTGAGGAGGA CCCTCTATCA CAAGGTGGTT GTATTCCATT TTATCGATCA 
TCTTCTGCCT GTGGCACAGG GCGTGGGGGT TTAAAGTTTG GAAACCCCTC ATTATTAAAC 
CCAAGAGAGC AAATAAATGG ATTAACATCA TTTATTGATG CATCAACTGT TTATGGAAGC 
TCAGAATCAT TACAACACAA GCTGAAAAAC CTTTCTAGTG AGGAAGGCCT CTTGAGAGTA 
AATGTAAAAT ATTCTGATTC CAGTCGAGAG TATCTGCCTT TTGAGACACC ATCAGCATGT 
ATGCAGGACC CTACAGCAGA TAATGCTGAG AGAATAGACT GTTTCTTTGC AGGTGAAGGC 
AGGGCCAATG AAGTTATAAC CCTGGCAGCA GTTCACACAT TATGGTTAAG AGAGCACAAC 
CGCATAGCCA AAGCCTTGAA AAAACTAAAT CCTCACTGGA ATTCAGAAAC CACTTATCAG 
GAGGCTCGGA AAATTGTTGG TGCCCTTCAT CAGATAATTA CTTTTCGAGA TTACATGCCA 
AAAATTCTGG GTAAAGCCGC ATATGATCAG TATATTGGTC TGTACAAAGG ATACAATCAA 
AAAACAAATC CCTCTATATC AAATATATTT ACAACGGCGG CATTTCGTTT TGGGCATGCA 
ACTATTCCAC CGATGGTGCA CAGACTCAAT TCTCAGTATG TAGATGATCC AAAATATCCC 
AGTTTGCCCC TGAATGAAGT TTTCTTTCGC CCATGGAGGA TTGTAAAAGA AGGAGGCTTG 
GATCCATTGA TTAGAGGACT TCTTGGAAAA TCAGCAAAAC TGCAAACACA AGTAGACATG 
ATCAATGAGG AATTAACAGA AAAACTGCTT GTAATGACCA ATAATGGTTC GATGGACTTA 
GCTTCCTTGA ATCTACAAAG AGGTCGAGAT CATGGCCTAC CAGGCTACAA TGACTGGAGG 
GAATTTTGTG GTTTATCAAG ACTGGCGACT CCAGCAGATC TAATAAATGC AGTATCTGAT 
CAGAAATTAG TAGCCAAAAT GATTGCTTTG TATAGTCATC CCGACAACAT TGACGTGTGG 
CTCGGGGGTC TAGCAGAGGA CTTCCTTCCA GGAGCTAGAA CTGGACCTCT TTTTGCATGT 
TTGATTGGAA AGCAAATGCA GGCACTGCGG GAAGGAGACA GGTTTTGGTA TGAAAACAAC 
AACATTTTTA CGAAAATTCA GAGATCTGAA CTAGAAAAAC ATTCTCTTTC GCGTGTAATT 
TGTGATAATA CAGGTTTATC CCATGTTCCC CTCGATGCCT TTCTACTGGG AAATTATCCA 
GACAACTTTG TGTCATGTGA CAGTATCCCT GGCATAAACC TGGAAGCCTG GAAAGAATCT 
CCTGAAAAAG GCACAACCTG TGGATCCCCA AGAAAAATAG AAAATGGAGA TTTTGCATTT 
TGTTCAGAGG CTACTGTAAT TTATTCTTGC CACAGCGGGT ATCGCTTAGA AGGCCATGAA 
GAAATAACCT GTCAAGGAAA TGAATGGAGT AACCAACCAC CCATTTGTTC AGATATCAAT 
GAATGTGAAG ATCAGCCTAA TGGCCCATGC CATTCTTCTG CTAAATGCAA GAACACATTG 
GGCGGCTTTC ATTGCTTATG CACAGATCCA TATGAACTTG ACAAGGATAA AAGGACATGC 
GTGGATTCAG GAAGACTCCC TAGAGGATCT TTAGTGTCCA TCATTTTAGT TGCTGTCTTG 
TTTGTGTCCT GGGCCGGAAT GTGCTGGATT CTAATTTGCA GAGGAGCATC CATATTTTGG 
TCATTGACTA AAGCTCACAA TGTGGAAGGT GGGAAGAAGC CATCGGTAGT GATGATTTAC 
TCCAGGTGTG AAAGTAAAAC CATGCCATTA CCATTGATTC CATCTGAACA AGTGGCCAAG 
GATGTAAGCA ATGGACACCA AATTCTACTT CATGTATAA