pum1.S cDNA ORF clone, Xenopus laevis(African clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	pum1.S
Entrez Gene ID	394392
Full Name	pumilio RNA binding family member 1 S homeolog
Synonyms	XPum,pum1,pum1-A,pumh,pumh1,pumilio,pumilio-1,pumilio1,puml1
General protein information	Preferred Names pumilio RNA binding family member 1 S homeolog Names pumilio homolog 1 pumilio 1 pumilio RNA-binding family member 1 pumilio-like protein 1
Gene Type	protein-coding
Organism	Xenopus laevis(African clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001087650.1	NP_001081119.1	pumilio homolog 1
XM_018248094.1	XP_018103583.1	pumilio homolog 1 isoform X1

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Daz- and pumilio-like genes are asymmetrically localized in Pelophylax (Rana) oocytes and are expressed during early spermatogenesis.
Marracci S, Michelotti V, Casola C, Giacoma C, Ragghianti M
Journal of experimental zoology. Part B, Molecular and developmental evolution316(5)330-8(2011 Jul)

Biochemical characterization of Pumilio1 and Pumilio2 in Xenopus oocytes.
Ota R, Kotani T, Yamashita M
The Journal of biological chemistry286(4)2853-63(2011 Jan)

Involvement of Xenopus Pumilio in the translational regulation that is specific to cyclin B1 mRNA during oocyte maturation.
Nakahata S, Kotani T, Mita K, Kawasaki T, Katsu Y, Nagahama Y, Yamashita M
Mechanisms of development120(8)865-80(2003 Aug)

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Klein SL, Strausberg RL, Wagner L, Pontius J, Clifton SW, Richardson P
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)

Biochemical identification of Xenopus Pumilio as a sequence-specific cyclin B1 mRNA-binding protein that physically interacts with a Nanos homolog, Xcat-2, and a cytoplasmic polyadenylation element-binding protein.
Nakahata S, Katsu Y, Mita K, Inoue K, Nagahama Y, Yamashita M
The Journal of biological chemistry276(24)20945-53(2001 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Daz- and pumilio-like genes are asymmetrically localized in Pelophylax (Rana) oocytes and are expressed during early spermatogenesis
Title: Daz- and pumilio-like genes are asymmetrically localized in Pelophylax (Rana) oocytes and are expressed during early spermatogenesis.

the functions of Pum1 and Pum2 in translational control of mRNAs during oocyte maturation.
Title: Biochemical characterization of Pumilio1 and Pumilio2 in Xenopus oocytes.

The following pum1.S gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the pum1.S cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAf21237	XM_018248094.1 Latest version!	Xenopus laevis pumilio RNA binding family member 1 S homeolog (pum1.S), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OAf00755	NM_001087650.1 Latest version!	Xenopus laevis pumilio RNA binding family member 1 S homeolog (pum1.S), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

pum1.S ( XM_018248094.1 ) cDNA ORF clone, Xenopus laevis(African clawed frog) -> XP_018103583.1 Xenopus laevis pumilio RNA binding family member 1 S homeolog (pum1.S), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OAf21237
Clone ID Related Accession (Same CDS sequence)	XM_018248094.1
Accession Version	XM_018248094.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3627bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-19
Organism	Xenopus laevis(African clawed frog)
Product	pumilio homolog 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030727.1) and transcript sequence (AB091091.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus laevis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 63 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGACCATTG GATGCTGGCA ACCTACTGCA GCTGTCAAAA CAAATTCCAC ATATTTTGGT 
GTTGGTGGAA TGAGCGTTGC ATGTGTCTTG AAGAGGAAAA CCGTTCTCTG GCAGGACTCC 
TTCAGCCCCC ACCTCAAACA GTACCCAGAA GGAACCCTTA ACCACAATAT GCCCGTGGTC 
CTTACATCTG GACCGGTGGG CCAGCAGCAG CCACCGCAGC CCCCCACTCA TTCTGCCCTG 
GCAACAGGTC CCCATGCCAG CCCTGTGGGG GGCTCTATGG GAGTGGCTGG ACGCTCACAG 
GATGATGCCA TGGTGGATTA TTTCTTCCAA AGGCAGCATG GGGAGCAGCT AGGTGGTGGC 
GGCAGTGGAG GAGGAGGCTA CAATAATAGC AAGCATCGCT GGCCCACTGG GGATAATATC 
CACGCAGAGC ATCAGGTCCG CTCAATGGAT GAGCTTAATC ATGATTTTCA AGCGCTTGCA 
CTGGAAGGAA GAGCAATGGG AGAACAACTG TTGACTGGCA AAAAATTCTG GGAGACTGAT 
GATTCAAACA AGGATGGACC AAAAGGCATT TTTCTTGGGG ACCAGTGGCG CGAGAGCACG 
TGGGGGGCTT CAGATCATTC AGTCTCCCAA CCCATTATGG TTCAACGGAG GCCTGGCCAA 
GGATTTCATG TCAGCAGTGA GGTGAACTCT GTTCTAAGCC CTCGATCTGA GAGTGGTGGC 
TTGGGCGTCA GCATGGTGGA ATATGTGCTC AGCTCCTCAC CTGGAGACTC ATGTCTGCGA 
AAAGGAGCAT TTGGTCCACG AGATACAGAG GGAGATGAAA ATGAGAAGGT GGACAAAAAA 
AATAAAGGTG TCTATGAGGG GGACAAATTG GGAGATCTAA AAGAGGAAGC AGACAGCATG 
GATAAATGCA ATGGCTTACC AGTCCAAAAT GGAATTGATG GAGATGTAAA GGATTTCAGC 
CGCACACCTG GAAACTGTCA AGCCTCAGCA AGTGAAGTAG ACCTTCTTGG GTCCATCCAG 
AATGGCTCAG AAGGGCTTGT CCAATTGACT AATAACAATG GGGCCAAGCC AGTAGAGGAT 
TTCGGTGGTA TTGAGTCTCA GAGTGTTCAG TTAGATCCCA TGGAGCATGT TGGCATGGAA 
CCACTGCAGT TTGACTACCA GGGCAACCAA GTACCGGTGG ATTCAGGAGC TGCCGCTGTG 
GGTCTATTTG ACTACAACTC GCAGCAGCAG CTATTCCAAA GACCAAATGC TCTAACCGTT 
CAGCAGCTGA CCGCTGCACA ACAGCAACAG TATGCACTTG CAGCCGCGCA TCAGCAACAC 
ATAGGTTTAG CTCCCGCTGC CTTTGTTCCA AATCCCTACA TCATAAGTGC AGCACCTCCT 
GGAACGGACC CCTACGCAGC AGGGCTGGCA GCGGCTGCAA CACTCGGTCC AGCTGTTGTA 
CCCCACCAGT ACTATGGAGT GACGCCTTGG GGGGTGTATC CAGCCAACCT CTTTCAGCAG 
CAAGCAGCAG CTGCTGCTGC GGCAAGCAAC TCTGCTTCAC AGCAGAACAA TCCACAGAGT 
CAACAGGGCC AACAACAGGT GCTACGTGCA GGAGGCAACC AGAGACCGTT GACACCCAAC 
CAAAATCAGC AGGGGCAACA AACTGATCAG CTGGTGGCAG CAGCTGCGGT GAATTCTGCA 
CTTGCCTTTG GACAAGGACT AGCTGCAGGA ATGCCAGGAT ACCCTATGCT TGCACCTGCT 
GCTTACTATG ATCAGACAGG TGCCCTAGTT GTGAATACTG GAGCCAGGAA TGGTTTGGGT 
GGACCTGTTC GTCTAGTAGC ACCAGCATCT GTTATCATCA GCCAATCAGC AGCACAAGCA 
GCCGTTGCAG CAGCTGCTGC CTCTGCAAAT GGTCCAGCAG GTACTGCTAA TGGACCATTT 
CGGCAGTTAA ACTCTCAGCA GCCACAAGGC CAGCCTCAGC CTCAAGGCAA CCAGAATCTT 
GCATCCAGCT CCTTCTATGG CAACAGCTCA ATGAACAGTA ATTCACAGAG CAGCTCCTTG 
TTCTCACAGA GCTCTGGTCA GCCTGGAAAC AGTTCTTTGG GATTCGGCAG CAGCGGGTCT 
TTAGGAGCCA CACTGAGTTC AGCTCTTGGT GGATTTGGGA CTGCAGTTGC GAATTCTAAC 
ACGAACAGTG GTTCTCGTCG TGACTCTTTG ACTGGCAGCA GTGATATCTA TAAGAGAACT 
TCTAGCAGCC TGACACCCAT AGGTCACAGC TTCTACAATG GACTGGGCTT TTCATCTTCT 
CCAGGTCCTG TGGGAATGCC ACTTCCCAGT CAGGGTCCCA GTCACTCCCA AACACCTCCT 
CCATCCCTGT CTTCTCATGG GTCCTCCACC AGCCTCAACC TTGGAGGTTT AACGAATGGT 
AGTGGGCGAT ATATTTCTGC TGCACCAGGA GCGGAAGCAA AGTACCGAAG TGCCAGCAGT 
GCATCTAGCT TATTCAGCCC AAGTAGCACA CTCTTCCCAT CCTCCCGCTT GCGCTATGGT 
ATGTCAGATG TCATGCCCTC TGGTAGAAGC AGACTACTTG AAGATTTCCG GAACAACCGC 
TACCCAAATT TGCAGTTACG AGAAATCGCT GGCCACATCA TGGAGTTTTC CCAGGACCAG 
CATGGATCAA GGTTTATTCA GTTGAAGCTG GAGCGAGCTA CACCTGCAGA AAGGCAACTG 
GTGTTCAACG AGATCCTTCA AGCAGCTTAC CAGCTTATGG TGGATGTATT TGGGAATTAT 
GTCATACAGA AATTTTTTGA GTTTGGCAGC CTAGAGCAGA AGCTTGCACT TGCTGAACGG 
ATTCGTGGGC ACGTACTTTC ACTGGCATTG CAAATGTACG GATGTCGGGT GATACAAAAG 
GCATTGGAGT TTATTCCACC TGATCAGCAG GTTATTAATG AAATGGTCAG AGAGTTGGAT 
GGTCATGTCT TGAAGTGTGT AAAGGACCAG AATGGGAATC ACGTGGTTCA AAAATGCATA 
GAGTGTGTAC AGCCGCAGTC CCTTCAATTC ATTATTGATG CCTTCAAGAG CCAGGTATTT 
GCACTGTCTA CGCATCCGTA TGGCTGTCGA GTGATCCAGC GCATTTTGGA GCATTGCCTT 
CCTGAGCAGA CACTGCCTAT TTTAGAAGAA TTACACCAGC ACACGGAGCA ACTTGTTCAG 
GATCAGTATG GTAACTACGT TATTCAGCAT GTTTTGGAGC ATGGTCGGCC GGAGGACAAA 
AGTAAAATCG TTGCGGAGAT TCGTGGAAAC GTTCTAGTCC TGAGTCAGCA CAAATTTGCC 
AGTAATGTAG TTGAAAAGTG TGTGACACAT GCATCTCGTA CGGAAAGGGC CATGCTGATA 
GACGAGGTGT GCACAATGAA TGATGGGCCT CATAGTGCCT TATACACCAT GATGAAGGAC 
CAGTATGCTA ACTATGTTGT ACAGAAAATG ATCGATGTAG CAGAACCAGC TCAGCGCAAG 
ATTGTTATGC ACAAGATCAG ACCACATATT GCCACTCTTC GTAAATACAC ATATGGCAAG 
CACATCCTGG CCAAGTTGGA AAAGTACTAC ATGAAAAATG GAATGGATCT GGGTCCAATG 
TGTGGACCCT CAAATGGAAT CATTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_018103583.1
CDS	280..3906
Translation	MTIGCWQPTAAVKTNSTYFGVGGMSVACVLKRKTVLWQDSFSPHLKQYPEGTLNHNMPVVLTSGPVGQQQPPQPPTHSALATGPHASPVGGSMGVAGRSQDDAMVDYFFQRQHGEQLGGGGSGGGGYNNSKHRWPTGDNIHAEHQVRSMDELNHDFQALALEGRAMGEQLLTGKKFWETDDSNKDGPKGIFLGDQWRESTWGASDHSVSQPIMVQRRPGQGFHVSSEVNSVLSPRSESGGLGVSMVEYVLSSSPGDSCLRKGAFGPRDTEGDENEKVDKKNKGVYEGDKLGDLKEEADSMDKCNGLPVQNGIDGDVKDFSRTPGNCQASASEVDLLGSIQNGSEGLVQLTNNNGAKPVEDFGGIESQSVQLDPMEHVGMEPLQFDYQGNQVPVDSGAAAVGLFDYNSQQQLFQRPNALTVQQLTAAQQQQYALAAAHQQHIGLAPAAFVPNPYIISAAPPGTDPYAAGLAAAATLGPAVVPHQYYGVTPWGVYPANLFQQQAAAAAAASNSASQQNNPQSQQGQQQVLRAGGNQRPLTPNQNQQGQQTDQLVAAAAVNSALAFGQGLAAGMPGYPMLAPAAYYDQTGALVVNTGARNGLGGPVRLVAPASVIISQSAAQAAVAAAAASANGPAGTANGPFRQLNSQQPQGQPQPQGNQNLASSSFYGNSSMNSNSQSSSLFSQSSGQPGNSSLGFGSSGSLGATLSSALGGFGTAVANSNTNSGSRRDSLTGSSDIYKRTSSSLTPIGHSFYNGLGFSSSPGPVGMPLPSQGPSHSQTPPPSLSSHGSSTSLNLGGLTNGSGRYISAAPGAEAKYRSASSASSLFSPSSTLFPSSRLRYGMSDVMPSGRSRLLEDFRNNRYPNLQLREIAGHIMEFSQDQHGSRFIQLKLERATPAERQLVFNEILQAAYQLMVDVFGNYVIQKFFEFGSLEQKLALAERIRGHVLSLALQMYGCRVIQKALEFIPPDQQVINEMVRELDGHVLKCVKDQNGNHVVQKCIECVQPQSLQFIIDAFKSQVFALSTHPYGCRVIQRILEHCLPEQTLPILEELHQHTEQLVQDQYGNYVIQHVLEHGRPEDKSKIVAEIRGNVLVLSQHKFASNVVEKCVTHASRTERAMLIDEVCTMNDGPHSALYTMMKDQYANYVVQKMIDVAEPAQRKIVMHKIRPHIATLRKYTYGKHILAKLEKYYMKNGMDLGPMCGPSNGII

Target ORF information:

RefSeq Version	XM_018248094.1
Organism	Xenopus laevis(African clawed frog)
Definition	Xenopus laevis pumilio RNA binding family member 1 S homeolog (pum1.S), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018248094.1

ORF Insert Sequence:

ATGACCATTG GATGCTGGCA ACCTACTGCA GCTGTCAAAA CAAATTCCAC ATATTTTGGT 
GTTGGTGGAA TGAGCGTTGC ATGTGTCTTG AAGAGGAAAA CCGTTCTCTG GCAGGACTCC 
TTCAGCCCCC ACCTCAAACA GTACCCAGAA GGAACCCTTA ACCACAATAT GCCCGTGGTC 
CTTACATCTG GACCGGTGGG CCAGCAGCAG CCACCGCAGC CCCCCACTCA TTCTGCCCTG 
GCAACAGGTC CCCATGCCAG CCCTGTGGGG GGCTCTATGG GAGTGGCTGG ACGCTCACAG 
GATGATGCCA TGGTGGATTA TTTCTTCCAA AGGCAGCATG GGGAGCAGCT AGGTGGTGGC 
GGCAGTGGAG GAGGAGGCTA CAATAATAGC AAGCATCGCT GGCCCACTGG GGATAATATC 
CACGCAGAGC ATCAGGTCCG CTCAATGGAT GAGCTTAATC ATGATTTTCA AGCGCTTGCA 
CTGGAAGGAA GAGCAATGGG AGAACAACTG TTGACTGGCA AAAAATTCTG GGAGACTGAT 
GATTCAAACA AGGATGGACC AAAAGGCATT TTTCTTGGGG ACCAGTGGCG CGAGAGCACG 
TGGGGGGCTT CAGATCATTC AGTCTCCCAA CCCATTATGG TTCAACGGAG GCCTGGCCAA 
GGATTTCATG TCAGCAGTGA GGTGAACTCT GTTCTAAGCC CTCGATCTGA GAGTGGTGGC 
TTGGGCGTCA GCATGGTGGA ATATGTGCTC AGCTCCTCAC CTGGAGACTC ATGTCTGCGA 
AAAGGAGCAT TTGGTCCACG AGATACAGAG GGAGATGAAA ATGAGAAGGT GGACAAAAAA 
AATAAAGGTG TCTATGAGGG GGACAAATTG GGAGATCTAA AAGAGGAAGC AGACAGCATG 
GATAAATGCA ATGGCTTACC AGTCCAAAAT GGAATTGATG GAGATGTAAA GGATTTCAGC 
CGCACACCTG GAAACTGTCA AGCCTCAGCA AGTGAAGTAG ACCTTCTTGG GTCCATCCAG 
AATGGCTCAG AAGGGCTTGT CCAATTGACT AATAACAATG GGGCCAAGCC AGTAGAGGAT 
TTCGGTGGTA TTGAGTCTCA GAGTGTTCAG TTAGATCCCA TGGAGCATGT TGGCATGGAA 
CCACTGCAGT TTGACTACCA GGGCAACCAA GTACCGGTGG ATTCAGGAGC TGCCGCTGTG 
GGTCTATTTG ACTACAACTC GCAGCAGCAG CTATTCCAAA GACCAAATGC TCTAACCGTT 
CAGCAGCTGA CCGCTGCACA ACAGCAACAG TATGCACTTG CAGCCGCGCA TCAGCAACAC 
ATAGGTTTAG CTCCCGCTGC CTTTGTTCCA AATCCCTACA TCATAAGTGC AGCACCTCCT 
GGAACGGACC CCTACGCAGC AGGGCTGGCA GCGGCTGCAA CACTCGGTCC AGCTGTTGTA 
CCCCACCAGT ACTATGGAGT GACGCCTTGG GGGGTGTATC CAGCCAACCT CTTTCAGCAG 
CAAGCAGCAG CTGCTGCTGC GGCAAGCAAC TCTGCTTCAC AGCAGAACAA TCCACAGAGT 
CAACAGGGCC AACAACAGGT GCTACGTGCA GGAGGCAACC AGAGACCGTT GACACCCAAC 
CAAAATCAGC AGGGGCAACA AACTGATCAG CTGGTGGCAG CAGCTGCGGT GAATTCTGCA 
CTTGCCTTTG GACAAGGACT AGCTGCAGGA ATGCCAGGAT ACCCTATGCT TGCACCTGCT 
GCTTACTATG ATCAGACAGG TGCCCTAGTT GTGAATACTG GAGCCAGGAA TGGTTTGGGT 
GGACCTGTTC GTCTAGTAGC ACCAGCATCT GTTATCATCA GCCAATCAGC AGCACAAGCA 
GCCGTTGCAG CAGCTGCTGC CTCTGCAAAT GGTCCAGCAG GTACTGCTAA TGGACCATTT 
CGGCAGTTAA ACTCTCAGCA GCCACAAGGC CAGCCTCAGC CTCAAGGCAA CCAGAATCTT 
GCATCCAGCT CCTTCTATGG CAACAGCTCA ATGAACAGTA ATTCACAGAG CAGCTCCTTG 
TTCTCACAGA GCTCTGGTCA GCCTGGAAAC AGTTCTTTGG GATTCGGCAG CAGCGGGTCT 
TTAGGAGCCA CACTGAGTTC AGCTCTTGGT GGATTTGGGA CTGCAGTTGC GAATTCTAAC 
ACGAACAGTG GTTCTCGTCG TGACTCTTTG ACTGGCAGCA GTGATATCTA TAAGAGAACT 
TCTAGCAGCC TGACACCCAT AGGTCACAGC TTCTACAATG GACTGGGCTT TTCATCTTCT 
CCAGGTCCTG TGGGAATGCC ACTTCCCAGT CAGGGTCCCA GTCACTCCCA AACACCTCCT 
CCATCCCTGT CTTCTCATGG GTCCTCCACC AGCCTCAACC TTGGAGGTTT AACGAATGGT 
AGTGGGCGAT ATATTTCTGC TGCACCAGGA GCGGAAGCAA AGTACCGAAG TGCCAGCAGT 
GCATCTAGCT TATTCAGCCC AAGTAGCACA CTCTTCCCAT CCTCCCGCTT GCGCTATGGT 
ATGTCAGATG TCATGCCCTC TGGTAGAAGC AGACTACTTG AAGATTTCCG GAACAACCGC 
TACCCAAATT TGCAGTTACG AGAAATCGCT GGCCACATCA TGGAGTTTTC CCAGGACCAG 
CATGGATCAA GGTTTATTCA GTTGAAGCTG GAGCGAGCTA CACCTGCAGA AAGGCAACTG 
GTGTTCAACG AGATCCTTCA AGCAGCTTAC CAGCTTATGG TGGATGTATT TGGGAATTAT 
GTCATACAGA AATTTTTTGA GTTTGGCAGC CTAGAGCAGA AGCTTGCACT TGCTGAACGG 
ATTCGTGGGC ACGTACTTTC ACTGGCATTG CAAATGTACG GATGTCGGGT GATACAAAAG 
GCATTGGAGT TTATTCCACC TGATCAGCAG GTTATTAATG AAATGGTCAG AGAGTTGGAT 
GGTCATGTCT TGAAGTGTGT AAAGGACCAG AATGGGAATC ACGTGGTTCA AAAATGCATA 
GAGTGTGTAC AGCCGCAGTC CCTTCAATTC ATTATTGATG CCTTCAAGAG CCAGGTATTT 
GCACTGTCTA CGCATCCGTA TGGCTGTCGA GTGATCCAGC GCATTTTGGA GCATTGCCTT 
CCTGAGCAGA CACTGCCTAT TTTAGAAGAA TTACACCAGC ACACGGAGCA ACTTGTTCAG 
GATCAGTATG GTAACTACGT TATTCAGCAT GTTTTGGAGC ATGGTCGGCC GGAGGACAAA 
AGTAAAATCG TTGCGGAGAT TCGTGGAAAC GTTCTAGTCC TGAGTCAGCA CAAATTTGCC 
AGTAATGTAG TTGAAAAGTG TGTGACACAT GCATCTCGTA CGGAAAGGGC CATGCTGATA 
GACGAGGTGT GCACAATGAA TGATGGGCCT CATAGTGCCT TATACACCAT GATGAAGGAC 
CAGTATGCTA ACTATGTTGT ACAGAAAATG ATCGATGTAG CAGAACCAGC TCAGCGCAAG 
ATTGTTATGC ACAAGATCAG ACCACATATT GCCACTCTTC GTAAATACAC ATATGGCAAG 
CACATCCTGG CCAAGTTGGA AAAGTACTAC ATGAAAAATG GAATGGATCT GGGTCCAATG 
TGTGGACCCT CAAATGGAAT CATTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pum1.S ( NM_001087650.1 ) cDNA ORF clone, Xenopus laevis(African clawed frog) -> NP_001081119.1 Xenopus laevis pumilio RNA binding family member 1 S homeolog (pum1.S), mRNA.

Price & Availability↑

CloneID	OAf00755
Clone ID Related Accession (Same CDS sequence)	NM_001087650.1
Accession Version	NM_001087650.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3573bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Xenopus laevis(African clawed frog)
Product	pumilio homolog 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC080379.1. On Jul 25, 2007 this sequence version replaced NM_001087651.1. ##Evidence-Data-START## Transcript exon combination :: BC080379.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00012418, SAMD00012419 [ECO:0000348] ##Evidence-Data-END##

ATGAGCGTTG CATGTGTCTT GAAGAGGAAA ACCGTTCTCT GGCAGGACTC CTTCAGCCCC 
CACCTCAAAC AGTACCCAGA AGGAACCCTT AACCACAATA TGCCCGTGGT CCTTACATCT 
GGACCGGTGG GCCAGCAGCA GCCACCGCAG CCCCCCACTC ATTCTGCCCT GGCAACAGGT 
CCCCATGCCA GCCCTGTGGG GGGCTCTATG GGAGTGGCTG GACGCTCACA GGATGATGCC 
ATGGTGGATT ATTTCTTCCA AAGGCAGCAT GGGGAGCAGC TAGGTGGTGG CGGCAGTGGA 
GGAGGAGGCT ACAATAATAG CAAGCATCGC TGGCCCACTG GGGATAATAT CCACGCAGAG 
CATCAGGTCC GCTCAATGGA TGAGCTTAAT CATGATTTTC AAGCGCTTGC ACTGGAAGGA 
AGAGCAATGG GAGAACAACT GTTGACTGGC AAAAAATTCT GGGAGACTGA TGATTCAAAC 
AAGGATGGAC CAAAAGGCAT TTTTCTTGGG GACCAGTGGC GCGAGAGCAC GTGGGGGGCT 
TCAGATCATT CAGTCTCCCA ACCCATTATG GTTCAACGGA GGCCTGGCCA AGGATTTCAT 
GTCAGCAGTG AGGTGAACTC TGTTCTAAGC CCTCGATCTG AGAGTGGTGG CTTGGGCGTC 
AGCATGGTGG AATATGTGCT CAGCTCCTCA CCTGGAGACT CATGTCTGCG AAAAGGAGCA 
TTTGGTCCAC GAGATACAGA GGGAGATGAA AATGAGAAGG TGGACAAAAA AAATAAAGGT 
GTCTATGAGG GGGACAAATT GGGAGATCTA AAAGAGGAAG CAGACAGCAT GGATAAATGC 
AATGGCTTAC CAGTCCAAAA TGGAATTGAT GGAGATGTAA AGGATTTCAG CCGCACACCT 
GGAAACTGTC AAGCCTCAGC AAGTGAAGTA GACCTTCTTG GGTCCATCCA GAATGGCTCA 
GAAGGGCTTG TCCAATTGAC TAATAACAAT GGGGCCAAGC CAGTAGAGGA TTTCGGTGGT 
ATTGAGTCTC AGAGTGTTCA GTTAGATCCC ATGGAGCATG TTGGCATGGA ACCACTGCAG 
TTTGACTACC AGGGCAACCA AGTACCGGTG GATTCAGGAG CTGCCGCTGT GGGTCTATTT 
GACTACAACT CGCAGCAGCA GCTATTCCAA AGACCAAATG CTCTAACCGT TCAGCAGCTG 
ACCGCTGCAC AACAGCAACA GTATGCACTT GCAGCCGCGC ATCAGCAACA CATAGGTATG 
TTTTCAGCAG GTTTAGCTCC CGCTGCCTTT GTTCCAAATC CCTACATCAT AAGTGCAGCA 
CCTCCTGGAA CGGACCCCTA CGCAGCAGGG CTGGCAGCGG CTGCAACACT CGGTCCAGCT 
GTTGTACCCC ACCAGTACTA TGGAGTGACA CCTTGGGGGG TGTATCCAGC CAACCTCTTT 
CAGCAGCAAG CAGCAGCTGC TGCTGCGGCA AGCAACTCTG CTTCACAGCA GAACAATCCA 
CAGAGTCAAC AGGGCCAACA ACAGGTGCTA CGTGCAGGAG GCAACCAGAG ACCGTTGACA 
CCCAACCAAA ATCAGCAGGG GCAACAAACT GATCAGCTGG TGGCAGCAGC TGCGGTGAAT 
TCTGCACTTG CCTTTGGACA AGGACTAGCT GCAGGAATGC CAGGATACCC TATGCTTGCA 
CCTGCTGCTT ACTATGATCA GACAGGTGCC CTAGTTGTGA ATACTGGAGC CAGGAATGGT 
TTGGGTGGAC CTGTTCGTCT AGTAGCACCA GCATCTGTTA TCATCAGCCA ATCAGCAGCA 
CAAGCAGCCG TTGCAGCAGC TGCTGCCTCT GCAAATGGTC CAGCAGGTAC TGCTAATGGA 
CCATTTCGGC AGTTAAACTC TCAGCAGCCA CAAGGCCAGC CTCAGCCTCA AGGCAACCAG 
AATCTTGCAT CCAGCTCCTT CTATGGCAAC AGCTCAATGA ACAGTAATTC ACAGAGCAGC 
TCCTTGTTCT CACAGAGCTC TGGTCAGCCT GGAAACAGTT CTTTGGGATT CGGCAGCAGC 
GGGTCTTTAG GAGCCACACT GAGTTCAGCT CTTGGTGGAT TTGGGACTGC AGTTGCGAAT 
TCTAACACGA ACAGTGGTTC TCGTCGTGAC TCTTTGACTG GCAGCAGTGA TATCTATAAG 
AGAACTTCTA GCAGCCTGAC ACCCATAGGT CACAGCTTCT ACAATGGTCT GGGCTTTTCA 
TCTTCTCCAG GTCCTGTGGG AATGCCACTT CCCAGTCAGG GTCCCAGTCA CTCCCAAACA 
CCTCCTCCAT CCCTGTCTTC TCATGGGTCC TCCACCAGCC TCAACCTTGG AGGTTTAACG 
AATGGTAGTG GGCGATATAT TTCTGCTGCA CCAGGAGCGG AAGCAAAGTA CCGAAGTGCC 
AGCAGTGCAT CTAGCTTATT CAGCCCAAGT AGCACACTCT TCCCATCCTC CCGCTTGCGC 
TATGGTATGT CAGATGTCAT GCCCTCTGGT AGAAGCAGAC TACTTGAAGA TTTCCGGAAC 
AACCGCTACC CAAATTTGCA GTTACGAGAA ATCGCTGGCC ACATCATGGA GTTTTCCCAG 
GACCAGCATG GATCAAGGTT TATTCAGTTG AAGCTGGAGC GAGCTACACC TGCAGAAAGG 
CAACTGGTGT TCAACGAGAT CCTTCAAGCA GCTTACCAGC TTATGGTGGA TGTATTTGGG 
AATTATGTCA TACAGAAATT TTTTGAGTTT GGCAGCCTAG AGCAGAAGCT TGCACTTGCT 
GAACGGATTC GTGGGCACGT ACTTTCACTG GCATTGCAAA TGTACGGATG TCGGGTGATA 
CAAAAGGCAT TGGAGTTTAT TCCACCTGAT CAGCAGGTTA TTAATGAAAT GGTCAGAGAG 
TTGGATGGTC ATGTCTTGAA GTGTGTAAAG GACCAGAATG GGAATCACGT GGTTCAAAAA 
TGCATAGAGT GTGTACAGCC GCAGTCCCTT CAATTCATTA TTGATGCCTT CAAGAGCCAG 
GTATTTGCAC TGTCTACGCA TCCGTATGGC TGTCGAGTGA TCCAGCGCAT TTTGGAGCAT 
TGCCTTCCTG AGCAGACACT GCCTATTTTA GAAGAATTAC ACCAGCACAC GGAGCAACTT 
GTTCAGGATC AGTATGGTAA CTACGTTATT CAGCATGTTT TGGAGCATGG TCGGCCGGAG 
GACAAAAGTA AAATCGTTGC GGAGATTCGT GGAAATGTTC TAGTCCTGAG TCAGCACAAA 
TTTGCCAGTA ATGTAGTTGA AAAGTGTGTG ACACATGCAT CTCGTACGGA AAGGGCCATG 
CTGATAGACG AGGTGTGCAC AATGAATGAT GGGCCTCATA GTGCCTTATA CACCATGATG 
AAGGACCAGT ATGCTAACTA TGTTGTACAG AAAATGATCG ATGTAGCAGA ACCAGCTCAG 
CGCAAGATTG TTATGCACAA GATCAGACCA CATATTGCCA CTCTTCGTAA ATACACATAT 
GGCAAGCACA TCCTGGCCAA GTTGGAAAAG TACTACATGA AAAATGGAAT GGATCTGGGT 
CCAATGTGTG GACCCTCAAA TGGAATCATT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001081119.1
CDS	75..3647
Translation	MSVACVLKRKTVLWQDSFSPHLKQYPEGTLNHNMPVVLTSGPVGQQQPPQPPTHSALATGPHASPVGGSMGVAGRSQDDAMVDYFFQRQHGEQLGGGGSGGGGYNNSKHRWPTGDNIHAEHQVRSMDELNHDFQALALEGRAMGEQLLTGKKFWETDDSNKDGPKGIFLGDQWRESTWGASDHSVSQPIMVQRRPGQGFHVSSEVNSVLSPRSESGGLGVSMVEYVLSSSPGDSCLRKGAFGPRDTEGDENEKVDKKNKGVYEGDKLGDLKEEADSMDKCNGLPVQNGIDGDVKDFSRTPGNCQASASEVDLLGSIQNGSEGLVQLTNNNGAKPVEDFGGIESQSVQLDPMEHVGMEPLQFDYQGNQVPVDSGAAAVGLFDYNSQQQLFQRPNALTVQQLTAAQQQQYALAAAHQQHIGMFSAGLAPAAFVPNPYIISAAPPGTDPYAAGLAAAATLGPAVVPHQYYGVTPWGVYPANLFQQQAAAAAAASNSASQQNNPQSQQGQQQVLRAGGNQRPLTPNQNQQGQQTDQLVAAAAVNSALAFGQGLAAGMPGYPMLAPAAYYDQTGALVVNTGARNGLGGPVRLVAPASVIISQSAAQAAVAAAAASANGPAGTANGPFRQLNSQQPQGQPQPQGNQNLASSSFYGNSSMNSNSQSSSLFSQSSGQPGNSSLGFGSSGSLGATLSSALGGFGTAVANSNTNSGSRRDSLTGSSDIYKRTSSSLTPIGHSFYNGLGFSSSPGPVGMPLPSQGPSHSQTPPPSLSSHGSSTSLNLGGLTNGSGRYISAAPGAEAKYRSASSASSLFSPSSTLFPSSRLRYGMSDVMPSGRSRLLEDFRNNRYPNLQLREIAGHIMEFSQDQHGSRFIQLKLERATPAERQLVFNEILQAAYQLMVDVFGNYVIQKFFEFGSLEQKLALAERIRGHVLSLALQMYGCRVIQKALEFIPPDQQVINEMVRELDGHVLKCVKDQNGNHVVQKCIECVQPQSLQFIIDAFKSQVFALSTHPYGCRVIQRILEHCLPEQTLPILEELHQHTEQLVQDQYGNYVIQHVLEHGRPEDKSKIVAEIRGNVLVLSQHKFASNVVEKCVTHASRTERAMLIDEVCTMNDGPHSALYTMMKDQYANYVVQKMIDVAEPAQRKIVMHKIRPHIATLRKYTYGKHILAKLEKYYMKNGMDLGPMCGPSNGII

Target ORF information:

RefSeq Version	NM_001087650.1
Organism	Xenopus laevis(African clawed frog)
Definition	Xenopus laevis pumilio RNA binding family member 1 S homeolog (pum1.S), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001087650.1

ORF Insert Sequence:

ATGAGCGTTG CATGTGTCTT GAAGAGGAAA ACCGTTCTCT GGCAGGACTC CTTCAGCCCC 
CACCTCAAAC AGTACCCAGA AGGAACCCTT AACCACAATA TGCCCGTGGT CCTTACATCT 
GGACCGGTGG GCCAGCAGCA GCCACCGCAG CCCCCCACTC ATTCTGCCCT GGCAACAGGT 
CCCCATGCCA GCCCTGTGGG GGGCTCTATG GGAGTGGCTG GACGCTCACA GGATGATGCC 
ATGGTGGATT ATTTCTTCCA AAGGCAGCAT GGGGAGCAGC TAGGTGGTGG CGGCAGTGGA 
GGAGGAGGCT ACAATAATAG CAAGCATCGC TGGCCCACTG GGGATAATAT CCACGCAGAG 
CATCAGGTCC GCTCAATGGA TGAGCTTAAT CATGATTTTC AAGCGCTTGC ACTGGAAGGA 
AGAGCAATGG GAGAACAACT GTTGACTGGC AAAAAATTCT GGGAGACTGA TGATTCAAAC 
AAGGATGGAC CAAAAGGCAT TTTTCTTGGG GACCAGTGGC GCGAGAGCAC GTGGGGGGCT 
TCAGATCATT CAGTCTCCCA ACCCATTATG GTTCAACGGA GGCCTGGCCA AGGATTTCAT 
GTCAGCAGTG AGGTGAACTC TGTTCTAAGC CCTCGATCTG AGAGTGGTGG CTTGGGCGTC 
AGCATGGTGG AATATGTGCT CAGCTCCTCA CCTGGAGACT CATGTCTGCG AAAAGGAGCA 
TTTGGTCCAC GAGATACAGA GGGAGATGAA AATGAGAAGG TGGACAAAAA AAATAAAGGT 
GTCTATGAGG GGGACAAATT GGGAGATCTA AAAGAGGAAG CAGACAGCAT GGATAAATGC 
AATGGCTTAC CAGTCCAAAA TGGAATTGAT GGAGATGTAA AGGATTTCAG CCGCACACCT 
GGAAACTGTC AAGCCTCAGC AAGTGAAGTA GACCTTCTTG GGTCCATCCA GAATGGCTCA 
GAAGGGCTTG TCCAATTGAC TAATAACAAT GGGGCCAAGC CAGTAGAGGA TTTCGGTGGT 
ATTGAGTCTC AGAGTGTTCA GTTAGATCCC ATGGAGCATG TTGGCATGGA ACCACTGCAG 
TTTGACTACC AGGGCAACCA AGTACCGGTG GATTCAGGAG CTGCCGCTGT GGGTCTATTT 
GACTACAACT CGCAGCAGCA GCTATTCCAA AGACCAAATG CTCTAACCGT TCAGCAGCTG 
ACCGCTGCAC AACAGCAACA GTATGCACTT GCAGCCGCGC ATCAGCAACA CATAGGTATG 
TTTTCAGCAG GTTTAGCTCC CGCTGCCTTT GTTCCAAATC CCTACATCAT AAGTGCAGCA 
CCTCCTGGAA CGGACCCCTA CGCAGCAGGG CTGGCAGCGG CTGCAACACT CGGTCCAGCT 
GTTGTACCCC ACCAGTACTA TGGAGTGACA CCTTGGGGGG TGTATCCAGC CAACCTCTTT 
CAGCAGCAAG CAGCAGCTGC TGCTGCGGCA AGCAACTCTG CTTCACAGCA GAACAATCCA 
CAGAGTCAAC AGGGCCAACA ACAGGTGCTA CGTGCAGGAG GCAACCAGAG ACCGTTGACA 
CCCAACCAAA ATCAGCAGGG GCAACAAACT GATCAGCTGG TGGCAGCAGC TGCGGTGAAT 
TCTGCACTTG CCTTTGGACA AGGACTAGCT GCAGGAATGC CAGGATACCC TATGCTTGCA 
CCTGCTGCTT ACTATGATCA GACAGGTGCC CTAGTTGTGA ATACTGGAGC CAGGAATGGT 
TTGGGTGGAC CTGTTCGTCT AGTAGCACCA GCATCTGTTA TCATCAGCCA ATCAGCAGCA 
CAAGCAGCCG TTGCAGCAGC TGCTGCCTCT GCAAATGGTC CAGCAGGTAC TGCTAATGGA 
CCATTTCGGC AGTTAAACTC TCAGCAGCCA CAAGGCCAGC CTCAGCCTCA AGGCAACCAG 
AATCTTGCAT CCAGCTCCTT CTATGGCAAC AGCTCAATGA ACAGTAATTC ACAGAGCAGC 
TCCTTGTTCT CACAGAGCTC TGGTCAGCCT GGAAACAGTT CTTTGGGATT CGGCAGCAGC 
GGGTCTTTAG GAGCCACACT GAGTTCAGCT CTTGGTGGAT TTGGGACTGC AGTTGCGAAT 
TCTAACACGA ACAGTGGTTC TCGTCGTGAC TCTTTGACTG GCAGCAGTGA TATCTATAAG 
AGAACTTCTA GCAGCCTGAC ACCCATAGGT CACAGCTTCT ACAATGGTCT GGGCTTTTCA 
TCTTCTCCAG GTCCTGTGGG AATGCCACTT CCCAGTCAGG GTCCCAGTCA CTCCCAAACA 
CCTCCTCCAT CCCTGTCTTC TCATGGGTCC TCCACCAGCC TCAACCTTGG AGGTTTAACG 
AATGGTAGTG GGCGATATAT TTCTGCTGCA CCAGGAGCGG AAGCAAAGTA CCGAAGTGCC 
AGCAGTGCAT CTAGCTTATT CAGCCCAAGT AGCACACTCT TCCCATCCTC CCGCTTGCGC 
TATGGTATGT CAGATGTCAT GCCCTCTGGT AGAAGCAGAC TACTTGAAGA TTTCCGGAAC 
AACCGCTACC CAAATTTGCA GTTACGAGAA ATCGCTGGCC ACATCATGGA GTTTTCCCAG 
GACCAGCATG GATCAAGGTT TATTCAGTTG AAGCTGGAGC GAGCTACACC TGCAGAAAGG 
CAACTGGTGT TCAACGAGAT CCTTCAAGCA GCTTACCAGC TTATGGTGGA TGTATTTGGG 
AATTATGTCA TACAGAAATT TTTTGAGTTT GGCAGCCTAG AGCAGAAGCT TGCACTTGCT 
GAACGGATTC GTGGGCACGT ACTTTCACTG GCATTGCAAA TGTACGGATG TCGGGTGATA 
CAAAAGGCAT TGGAGTTTAT TCCACCTGAT CAGCAGGTTA TTAATGAAAT GGTCAGAGAG 
TTGGATGGTC ATGTCTTGAA GTGTGTAAAG GACCAGAATG GGAATCACGT GGTTCAAAAA 
TGCATAGAGT GTGTACAGCC GCAGTCCCTT CAATTCATTA TTGATGCCTT CAAGAGCCAG 
GTATTTGCAC TGTCTACGCA TCCGTATGGC TGTCGAGTGA TCCAGCGCAT TTTGGAGCAT 
TGCCTTCCTG AGCAGACACT GCCTATTTTA GAAGAATTAC ACCAGCACAC GGAGCAACTT 
GTTCAGGATC AGTATGGTAA CTACGTTATT CAGCATGTTT TGGAGCATGG TCGGCCGGAG 
GACAAAAGTA AAATCGTTGC GGAGATTCGT GGAAATGTTC TAGTCCTGAG TCAGCACAAA 
TTTGCCAGTA ATGTAGTTGA AAAGTGTGTG ACACATGCAT CTCGTACGGA AAGGGCCATG 
CTGATAGACG AGGTGTGCAC AATGAATGAT GGGCCTCATA GTGCCTTATA CACCATGATG 
AAGGACCAGT ATGCTAACTA TGTTGTACAG AAAATGATCG ATGTAGCAGA ACCAGCTCAG 
CGCAAGATTG TTATGCACAA GATCAGACCA CATATTGCCA CTCTTCGTAA ATACACATAT 
GGCAAGCACA TCCTGGCCAA GTTGGAAAAG TACTACATGA AAAATGGAAT GGATCTGGGT 
CCAATGTGTG GACCCTCAAA TGGAATCATT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Daz- and pumilio-like genes are asymmetrically localized in Pelophylax (Rana) oocytes and are expressed during early spermatogenesis.
Journal of experimental zoology. Part B, Molecular and developmental evolution316(5)330-8(2011 Jul)
Marracci S,Michelotti V,Casola C,Giacoma C,Ragghianti M

Biochemical characterization of Pumilio1 and Pumilio2 in Xenopus oocytes.
The Journal of biological chemistry286(4)2853-63(2011 Jan)
Ota R,Kotani T,Yamashita M

Involvement of Xenopus Pumilio in the translational regulation that is specific to cyclin B1 mRNA during oocyte maturation.
Mechanisms of development120(8)865-80(2003 Aug)
Nakahata S,Kotani T,Mita K,Kawasaki T,Katsu Y,Nagahama Y,Yamashita M

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)
Klein SL,Strausberg RL,Wagner L,Pontius J,Clifton SW,Richardson P

Biochemical identification of Xenopus Pumilio as a sequence-specific cyclin B1 mRNA-binding protein that physically interacts with a Nanos homolog, Xcat-2, and a cytoplasmic polyadenylation element-binding protein.
The Journal of biological chemistry276(24)20945-53(2001 Jun)
Nakahata S,Katsu Y,Mita K,Inoue K,Nagahama Y,Yamashita M