twsg1.L cDNA ORF clone, Xenopus laevis(African clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	twsg1.L
Entrez Gene ID	379560
Full Name	twisted gastrulation BMP signaling modulator 1 L homeolog
Synonyms	tsg,twsg,twsg1,twsg1-a,twsg1-b
General protein information	Preferred Names twisted gastrulation BMP signaling modulator 1 L homeolog Names twisted gastrulation protein homolog 1-B twisted gastrulation homolog 1
Gene Type	protein-coding
Organism	Xenopus laevis(African clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001086401.1	NP_001079870.1	twisted gastrulation protein homolog 1-B precursor

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Sirenomelia in Bmp7 and Tsg compound mutant mice: requirement for Bmp signaling in the development of ventral posterior mesoderm.
Zakin L, Reversade B, Kuroda H, Lyons KM, De Robertis EM
Development (Cambridge, England)132(10)2489-99(2005 May)

Identification of a second Xenopus twisted gastrulation gene.
Oelgeschl?ger M, Tran U, Grubisic K, De Robertis EM
The International journal of developmental biology48(1)57-61(2004 Feb)

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Klein SL, Strausberg RL, Wagner L, Pontius J, Clifton SW, Richardson P
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)

Evolutionary conservation, developmental expression, and genomic mapping of mammalian Twisted gastrulation.
Graf D, Timmons PM, Hitchins M, Episkopou V, Moore G, Ito T, Fujiyama A, Fisher AG, Merkenschlager M
Mammalian genome : official journal of the International Mammalian Genome Society12(7)554-60(2001 Jul)

The evolutionarily conserved BMP-binding protein Twisted gastrulation promotes BMP signalling.
Oelgeschl?ger M, Larra?n J, Geissert D, De Robertis EM
Nature405(6788)757-63(2000 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

In microinjection experiments, the activity of xTsg-2 mRNA was similar to that of xTsg
Title: Identification of a second Xenopus twisted gastrulation gene.

The following twsg1.L gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the twsg1.L cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAf00349	NM_001086401.1 Latest version!	Xenopus laevis twisted gastrulation BMP signaling modulator 1 L homeolog (twsg1.L), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$174.30 $249.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

twsg1.L ( NM_001086401.1 ) cDNA ORF clone, Xenopus laevis(African clawed frog) -> NP_001079870.1 Xenopus laevis twisted gastrulation BMP signaling modulator 1 L homeolog (twsg1.L), mRNA.

Price & Availability↑

CloneID	OAf00349
Clone ID Related Accession (Same CDS sequence)	NM_001086401.1
Accession Version	NM_001086401.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 657bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-16
Organism	Xenopus laevis(African clawed frog)
Product	twisted gastrulation protein homolog 1-B precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF245221.1. ##Evidence-Data-START## Transcript exon combination :: AF245221.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00012418, SAMD00012419 [ECO:0000348] ##Evidence-Data-END##

ATGAAGCCCT CTTTCCTTCA TATTCCAGCG GCGGCTCTGC TCCTCTGCTC TCTATGGATT 
TTGCCCATTC ATTGCTGCAA TAAGGCACTT TGTGCAAGTG ATGTCAGCAA ATGTCTAATA 
CAGGAGCTGT GCCAGTGTCG GCCCACGGAT GGAAACTGCT CCTGCTGCAA GGAGTGTATG 
CTGTGCCTGG GCACACTCTG GGACGAGTGC TGTGACTGCG TTGGTATGTG CAACCCAAGG 
AACTACAGCG ATACGCCCCC AACCTCCAAA AGTACAGTAG AGGAACTTCA TGAACCCATT 
CCATCCTTGT TCCGGGCTCT AACTGAGGGA GACACCCAAC TTAATTGGAA TATTGTAACC 
TTTCCTGTAG TGGAGGAACT TTCCCATCAT GAGAACCTGG TCTCCTTCCT GGAAACTGTG 
AACCAACCAC AACAGCAAAA TGTATCTGTC CAGGTGTCGC ACAGCAATGA AAAGGAGCAC 
ATGTGTACAG TGGTCTATTT TGATGACTGC ATGTCAATCC ATCAGTGTAA GGTTTCCTGC 
GAGTCCATGG GAGCGTCAAA GTACCGGTGG TTCCACAACG CTTGCTGCGA ATGTGTGGGG 
CCCGAGTGTA TTGACTACGG AAGCAAAACA GTCAAGTGCG TGAACTGTAT GGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001079870.1
CDS	193..849
Translation	MKPSFLHIPAAALLLCSLWILPIHCCNKALCASDVSKCLIQELCQCRPTDGNCSCCKECMLCLGTLWDECCDCVGMCNPRNYSDTPPTSKSTVEELHEPIPSLFRALTEGDTQLNWNIVTFPVVEELSHHENLVSFLETVNQPQQQNVSVQVSHSNEKEHMCTVVYFDDCMSIHQCKVSCESMGASKYRWFHNACCECVGPECIDYGSKTVKCVNCMV

Target ORF information:

RefSeq Version	NM_001086401.1
Organism	Xenopus laevis(African clawed frog)
Definition	Xenopus laevis twisted gastrulation BMP signaling modulator 1 L homeolog (twsg1.L), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001086401.1

ORF Insert Sequence:

ATGAAGCCCT CTTTCCTTCA TATTCCAGCG GCGGCTCTGC TCCTCTGCTC TCTATGGATT 
TTGCCCATTC ATTGCTGCAA TAAGGCACTT TGTGCAAGTG ATGTCAGCAA ATGTCTAATA 
CAGGAGCTGT GCCAGTGTCG GCCCACGGAT GGAAACTGCT CCTGCTGCAA GGAGTGTATG 
CTGTGCCTGG GCACACTCTG GGACGAGTGC TGTGACTGCG TTGGTATGTG CAACCCAAGG 
AACTACAGCG ATACGCCCCC AACCTCCAAA AGTACAGTAG AGGAACTTCA TGAACCCATT 
CCATCCTTGT TCCGGGCTCT AACTGAGGGA GACACCCAAC TTAATTGGAA TATTGTAACC 
TTTCCTGTAG TGGAGGAACT TTCCCATCAT GAGAACCTGG TCTCCTTCCT GGAAACTGTG 
AACCAACCAC AACAGCAAAA TGTATCTGTC CAGGTGTCGC ACAGCAATGA AAAGGAGCAC 
ATGTGTACAG TGGTCTATTT TGATGACTGC ATGTCAATCC ATCAGTGTAA GGTTTCCTGC 
GAGTCCATGG GAGCGTCAAA GTACCGGTGG TTCCACAACG CTTGCTGCGA ATGTGTGGGG 
CCCGAGTGTA TTGACTACGG AAGCAAAACA GTCAAGTGCG TGAACTGTAT GGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sirenomelia in Bmp7 and Tsg compound mutant mice: requirement for Bmp signaling in the development of ventral posterior mesoderm.
Development (Cambridge, England)132(10)2489-99(2005 May)
Zakin L,Reversade B,Kuroda H,Lyons KM,De Robertis EM

Identification of a second Xenopus twisted gastrulation gene.
The International journal of developmental biology48(1)57-61(2004 Feb)
Oelgeschl?ger M,Tran U,Grubisic K,De Robertis EM

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)
Klein SL,Strausberg RL,Wagner L,Pontius J,Clifton SW,Richardson P

Evolutionary conservation, developmental expression, and genomic mapping of mammalian Twisted gastrulation.
Mammalian genome : official journal of the International Mammalian Genome Society12(7)554-60(2001 Jul)
Graf D,Timmons PM,Hitchins M,Episkopou V,Moore G,Ito T,Fujiyama A,Fisher AG,Merkenschlager M

The evolutionarily conserved BMP-binding protein Twisted gastrulation promotes BMP signalling.
Nature405(6788)757-63(2000 Jun)
Oelgeschl?ger M,Larra?n J,Geissert D,De Robertis EM