timp3.L cDNA ORF clone, Xenopus laevis(African clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	timp3.L
Entrez Gene ID	373596
Full Name	TIMP metallopeptidase inhibitor 3 L homeolog
Synonyms	timp3,timp3-A
General protein information	Preferred Names TIMP metallopeptidase inhibitor 3 L homeolog Names metalloproteinase inhibitor 3 TIMP-3 tissue inhibitor of metalloproteinases-3
Gene Type	protein-coding
Organism	Xenopus laevis(African clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001085595.1	NP_001079064.1	metalloproteinase inhibitor 3 precursor

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Domain specific overexpression of TIMP-2 and TIMP-3 reveals MMP-independent functions of TIMPs during Xenopus laevis development.
Nieuwesteeg MA, Walsh LA, Fox MA, Damjanovski S
Biochemistry and cell biology = Biochimie et biologie cellulaire90(4)585-95(2012 Aug)

Membrane type-1 matrix metalloproteinases and tissue inhibitor of metalloproteinases-2 RNA levels mimic each other during Xenopus laevis metamorphosis.
Walsh LA, Carere DA, Cooper CA, Damjanovski S
PloS one2(10)e1000(2007 Oct)

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Klein SL, Strausberg RL, Wagner L, Pontius J, Clifton SW, Richardson P
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)

Cloning and developmental regulation of tissue inhibitor of metalloproteinases-3 (TIMP3) in Xenopus laevis early embryos.
Yang M, Kurkinen M
Gene211(1)95-100(1998 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

only the N-terminal, but not the C-terminal domain of TIMP-3, results in developmental defects.
Title: Domain specific overexpression of TIMP-2 and TIMP-3 reveals MMP-independent functions of TIMPs during Xenopus laevis development.

metamorphic tail and intestine RNA levels of TIMP-2, MT1-MMP and Gel-A, but not MT3-MMP or TIMP-3, are elevated during periods of cell death and proliferation
Title: Membrane type-1 matrix metalloproteinases and tissue inhibitor of metalloproteinases-2 RNA levels mimic each other during Xenopus laevis metamorphosis.

The following timp3.L gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the timp3.L cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAf00032	NM_001085595.1 Latest version!	Xenopus laevis TIMP metallopeptidase inhibitor 3 L homeolog (timp3.L), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

timp3.L ( NM_001085595.1 ) cDNA ORF clone, Xenopus laevis(African clawed frog) -> NP_001079064.1 Xenopus laevis TIMP metallopeptidase inhibitor 3 L homeolog (timp3.L), mRNA.

Price & Availability↑

CloneID	OAf00032
Clone ID Related Accession (Same CDS sequence)	NM_001085595.1
Accession Version	NM_001085595.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 645bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-20
Organism	Xenopus laevis(African clawed frog)
Product	metalloproteinase inhibitor 3 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC060423.1. ##Evidence-Data-START## Transcript exon combination :: BC060423.1 [ECO:0000332] ##Evidence-Data-END##

ATGTCTGTGT GTGCTCTGAC CCTGATCTTG GGCTGCTTCC TGCTCTTTCT GGGGGACATC 
TCCAAACCAG CAGAGGGGTG CACGTGTGCC CCCAGCCACC CACAGGACGC CTTCTGCAAC 
TCTGACATAG TGATCCGGGC CAAGGTGGTG GGTAAAAAGT TGATGAAGGA CGGGCCATTC 
GGAACCATGC GTTACACAGT TAAGCAGATG AAGATGTACC GGGGCTTCAA CAAAATGCCG 
CAAGTCCAGT ACATCTACAC CGAGGCCTCG GAGAGCCTGT GTGGCGTGAA ACTGGAAGTC 
AACAAGTATC AGTATCTGAT AACTGGACGT GTATATGAAG GGAAGGTGTA CACCGGGCTG 
TGTAATTTAA TCGAGAGGTG GGAGAAACTG ACCTTCGCCC AAAGAAAGGG ACTGAATCAC 
CGCTATCCTC TTGGTTGCAC CTGTAAGATC AAGCCTTGCT ACTACCTTCC CTGTTTCATC 
ACTTCCAAGA ACGAGTGCCT CTGGACGGAC ATGCTCTCCA ACTTCGGCTA CCCTGGCTAC 
CAGTCTAAGA ACTACGCCTG CATCAAGCAG AAGGAAGGCT ACTGCAGCTG GTACAGAGGA 
TGGGCACCTC CAGACAAAAC CACAATAAAC ACCACGGATC CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001079064.1
CDS	115..759
Translation	MSVCALTLILGCFLLFLGDISKPAEGCTCAPSHPQDAFCNSDIVIRAKVVGKKLMKDGPFGTMRYTVKQMKMYRGFNKMPQVQYIYTEASESLCGVKLEVNKYQYLITGRVYEGKVYTGLCNLIERWEKLTFAQRKGLNHRYPLGCTCKIKPCYYLPCFITSKNECLWTDMLSNFGYPGYQSKNYACIKQKEGYCSWYRGWAPPDKTTINTTDP

Target ORF information:

RefSeq Version	NM_001085595.1
Organism	Xenopus laevis(African clawed frog)
Definition	Xenopus laevis TIMP metallopeptidase inhibitor 3 L homeolog (timp3.L), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001085595.1

ORF Insert Sequence:

ATGTCTGTGT GTGCTCTGAC CCTGATCTTG GGCTGCTTCC TGCTCTTTCT GGGGGACATC 
TCCAAACCAG CAGAGGGGTG CACGTGTGCC CCCAGCCACC CACAGGACGC CTTCTGCAAC 
TCTGACATAG TGATCCGGGC CAAGGTGGTG GGTAAAAAGT TGATGAAGGA CGGGCCATTC 
GGAACCATGC GTTACACAGT TAAGCAGATG AAGATGTACC GGGGCTTCAA CAAAATGCCG 
CAAGTCCAGT ACATCTACAC CGAGGCCTCG GAGAGCCTGT GTGGCGTGAA ACTGGAAGTC 
AACAAGTATC AGTATCTGAT AACTGGACGT GTATATGAAG GGAAGGTGTA CACCGGGCTG 
TGTAATTTAA TCGAGAGGTG GGAGAAACTG ACCTTCGCCC AAAGAAAGGG ACTGAATCAC 
CGCTATCCTC TTGGTTGCAC CTGTAAGATC AAGCCTTGCT ACTACCTTCC CTGTTTCATC 
ACTTCCAAGA ACGAGTGCCT CTGGACGGAC ATGCTCTCCA ACTTCGGCTA CCCTGGCTAC 
CAGTCTAAGA ACTACGCCTG CATCAAGCAG AAGGAAGGCT ACTGCAGCTG GTACAGAGGA 
TGGGCACCTC CAGACAAAAC CACAATAAAC ACCACGGATC CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Domain specific overexpression of TIMP-2 and TIMP-3 reveals MMP-independent functions of TIMPs during Xenopus laevis development.
Biochemistry and cell biology = Biochimie et biologie cellulaire90(4)585-95(2012 Aug)
Nieuwesteeg MA,Walsh LA,Fox MA,Damjanovski S

Membrane type-1 matrix metalloproteinases and tissue inhibitor of metalloproteinases-2 RNA levels mimic each other during Xenopus laevis metamorphosis.
PloS one2(10)e1000(2007 Oct)
Walsh LA,Carere DA,Cooper CA,Damjanovski S

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)
Klein SL,Strausberg RL,Wagner L,Pontius J,Clifton SW,Richardson P

Cloning and developmental regulation of tissue inhibitor of metalloproteinases-3 (TIMP3) in Xenopus laevis early embryos.
Gene211(1)95-100(1998 Apr)
Yang M,Kurkinen M