crim1.L cDNA ORF clone, Xenopus laevis(African clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	crim1.L
Entrez Gene ID	100316916
Full Name	cysteine rich transmembrane BMP regulator 1 (chordin-like) L homeolog
Synonyms	crim1,s52
General protein information	Preferred Names cysteine rich transmembrane BMP regulator 1 (chordin-like) L homeolog Names cysteine rich transmembrane BMP regulator 1 (chordin-like) L homeolog cysteine-rich motor neuron 1
Gene Type	protein-coding
Organism	Xenopus laevis(African clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001170446.1	NP_001163917.1	cysteine rich transmembrane BMP regulator 1 (chordin-like) L homeolog precursor
XM_018261588.1	XP_018117077.1	cysteine rich transmembrane BMP regulator 1 (chordin-like) L homeolog isoform X1

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

CRIM1 complexes with ?-catenin and cadherins, stabilizes cell-cell junctions and is critical for neural morphogenesis.
Ponferrada VG, Fan J, Vallance JE, Hu S, Mamedova A, Rankin SA, Kofron M, Zorn AM, Hegde RS, Lang RA
PloS one7(3)e32635(2012)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

CRIM1 complexes with ss-catenin and cadherins, stabilizes cell-cell junctions and is critical for neural morphogenesis.
Title: CRIM1 complexes with ?-catenin and cadherins, stabilizes cell-cell junctions and is critical for neural morphogenesis.

The following crim1.L gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the crim1.L cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAf31258	XM_018261588.1 Latest version!	Xenopus laevis cysteine rich transmembrane BMP regulator 1 (chordin-like) L homeolog (crim1.L), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OAf05350	NM_001170446.1 Latest version!	Xenopus laevis cysteine rich transmembrane BMP regulator 1 (chordin-like) L homeolog (crim1.L), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

crim1.L ( XM_018261588.1 ) cDNA ORF clone, Xenopus laevis(African clawed frog) -> XP_018117077.1 Xenopus laevis cysteine rich transmembrane BMP regulator 1 (chordin-like) L homeolog (crim1.L), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OAf31258
Clone ID Related Accession (Same CDS sequence)	XM_018261588.1
Accession Version	XM_018261588.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2907bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-19
Organism	Xenopus laevis(African clawed frog)
Product	cysteine rich transmembrane BMP regulator 1 (chordin-like) L homeolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030732.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus laevis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACCTGC CGGGGAAGTT ACTGCGTCTG TGGCTCCTGC TGCTGCTGGT TTTGACGCTG 
CTGCCAGCGT CTGCCTGGGC TTTGGTCTGC TTGCCCTGCG ACGAATCCAA ATGCGAGGAA 
CCCAAGAGTT GCCCGGGGAG TATAGTTCAA GGCATCTGCG GCTGCTGCTT CATGTGCGCC 
AGGCAGAAGA ACGAGAGCTG CGGGGGAGTC TATGGGCTAC ACGGAGCCTG CGACAGGGGT 
CTGCGCTGTG TCATCCGCCC CCCGCTTAAT GGCGACTCCA TTACAGAATA TGAAGTGGGG 
GTTTGCGAAG ATGAAAACTG GGATGATGAC CAATTGCATG GCTTCGTCCC TTGCAATGAA 
AACTTTGTAA CCGGTTGCAA CATCATCAAT GGGAAATGTG AATGCGATAC CATACGGACC 
TGTAACACTC CCTTTGAGTT CCCCAGCCAG CATGCCTGCC TGACTGCGCT GAGACGGATT 
GAAGAAGAGA GACCAGACTG CTCATCAGCT CGATGTGAGG TACAGTTCTC TCCACGTTGT 
GCCGAGGACT CCGTTTTGAT AGAAGGCTAT GCCCCACCTG GTGAATGCTG CCCGCTGCCA 
AGCCGATGTG TATGCAACCC AGCCGGATGT CTAAGGAAAG TTTGCCAGCC TGGTTACTTA 
AATATTTTGG TATCCAAAGC ATCTGGCAAG CCTGGGGAAT GTTGTGACCT TTATGAATGC 
AAACCAGTCT TCAGTGTGGA CTGCAGTACT GTGGAGTGCC CTCCAGTTCA ACAGGCCATA 
TGCCCATTGG ATAGCTATGA GACACAAGTC CGCCTTACAG CTGACGGATG CTGCACGCTG 
CCTACAAGAT GTGAGTGTCT CTCGGGCCTC TGCACTTTCC CTGTATGTGA TGCTGGATCA 
ATCCCCCGCA TAGTCTCTCG AGGAGATGGC ACACCTGGAA AGTGCTGTGA CGTCTTTGAA 
TGTGTTAATG ATCCTGTGTA CCCAGTCCAT GACCCAGCAG GTTGCTACGC CAATGGACAA 
ATAAGAAGTC ACGGGGACCG CTGGAGAGAA GATGACTGCA CCTTCTGCCA GTGTATCAAT 
GGAGAGCCTC ACTGTGTTGC AACAGCTTGT GGCCAGAGTT GCTTGAAACC AGTTAAAGTG 
CCTGGAGAAT GTTGTCCTGT GTGTGAAGAG CCTACATATA TCACTATGGC TCCACCCATC 
TGCGACCTGC TGATCAACTG CACGTTGACT GAAAAGGACT GCAGCTATGG TTTCCGATTA 
GATCAAAATG GATGCCGCAC ATGCCAATGC AAAACAAGAG AGGAGCTCTG TACTGGTCTC 
ATATCAGGCT GTGCATTGGA CTGCCCTTTT GGATTTCAGA CTCATCTACA CAACTGTGAG 
ATCTGCCAGT GTCGACCTCG GCCCAAGAAG TGCAAACCTT TAGTGTGTGA CAAATACTGT 
CCGTTTGGCT ACCTGAAGAA CAAGCATGGC TGTGAAACAT GTCGCTGTAA GAAGTGCCCT 
GATGCCCCTT GCAGCAAAAT CTGCCCAATG GGATTTGAAC AGGACAATCA CGGATGCCGC 
ATCTGCAAAT GCAGGGAAAC AATGACATCT GTGGTACCCC CAGTCAAAAC AGGCTCATGC 
TTGTCAATGG ATGGTCGACG CCATGAAAAT GAAGAGAGTT GGCATGATGG ATGTCGTGAG 
TGCTATTGTC ACAATGGCAA AGAAATGTGT GGACTCATTA CATGTCCTGT TCCTGATTGT 
GTGAACCCCA CTATATACCC AGGACAATGC TGCCCTTCAT GTCCAGATGA TTCCAATGCA 
CAGAATCCAG AACTTACAGA CCCATCCATT TGCCATGCTC CTGGAGGGGA GTACTTTGTT 
GAAGGGGAAA CCTGGAATAT TGACACATGC ACCCAGTGCA CTTGCCATAG TGGGCGGGTG 
CTTTGTGAAA CTGAGGTCTG CCCACCTCTT CTTTGCCAGA ACCCAACACG TTCCCAGGAT 
TCTTGCTGTC CTCAATGCCC AGATGATTCC CTGCAGCCTT CTGTCCCAAG CAACGACAGT 
GTCCCCAGCT ACTGCAAAAA TGACGAGGGC GACATTTTCC TGGCTGCTGA GTCTTGGAAA 
CCTAATGTCT GTACAAGCTG CGTCTGCATG GACGGGATTA TCAGCTGCTA CTCTGAATCC 
TGCCCCCCTG TAACCTGTGA GAGACCGGTT TTAAGGAAGG GTCAGTGCTG TCCATATTGC 
ATAGAAGACA CAATTCCAAA GAAGGTGGTG TGCCATTTCA ATGGCAAAAC ATATGCGGAT 
GAGGAGCGGT GGGACATTGA CAGCTGCACA CATTGCTATT GCCTTCAAGG ACAGACCCTC 
TGCTCTACTG TGAGCTGCCC CCCTCTTCCC TGTGTGGAGC CAATGAATGT TGAAAGTAGC 
TGTTGTCCTA TGTGTCCAGA AATGTATATA CCGGAACCCA CAAATATCCC TATTGAGAAG 
ACAAACCACA GAGGAAAGAA TGAGCTAGAC ATGCCTCACT GGGCAACTCC TAGCGAGAAT 
GACATCGTTC ATCACCACAG AGACATGGGT CACCTTCAGG CAAACTACAG AGAAGTGGAA 
AGACCCCGTT TGACCGAAGA AGACTCTCTT CATTCTGTGG CATGGGTTGC GGTCCCTATC 
ATGATTGCGT TGGTTATCCT AATTGTTCTT TTTATGATTA ATCAGAAAAA ACAGTGGATA 
CCAGTACCCT GTTATAGAAC GCCTAACAAG CCGACCTGTC TGAACAATCA GCTTGTGTAT 
GTGGACTGCA AAAAAGGAAC CATGGTTCAA GTGGACAGCT CCCAGAGAAT GTTGAGAATG 
GCAGACCCGG ACTCAAGATA CAGCGGATTC TACAGTATGC AAAAACAAAA TAACCTCCAG 
GCAGATAATT TTTACCAAAC TGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_018117077.1
CDS	913..3819
Translation	MYLPGKLLRLWLLLLLVLTLLPASAWALVCLPCDESKCEEPKSCPGSIVQGICGCCFMCARQKNESCGGVYGLHGACDRGLRCVIRPPLNGDSITEYEVGVCEDENWDDDQLHGFVPCNENFVTGCNIINGKCECDTIRTCNTPFEFPSQHACLTALRRIEEERPDCSSARCEVQFSPRCAEDSVLIEGYAPPGECCPLPSRCVCNPAGCLRKVCQPGYLNILVSKASGKPGECCDLYECKPVFSVDCSTVECPPVQQAICPLDSYETQVRLTADGCCTLPTRCECLSGLCTFPVCDAGSIPRIVSRGDGTPGKCCDVFECVNDPVYPVHDPAGCYANGQIRSHGDRWREDDCTFCQCINGEPHCVATACGQSCLKPVKVPGECCPVCEEPTYITMAPPICDLLINCTLTEKDCSYGFRLDQNGCRTCQCKTREELCTGLISGCALDCPFGFQTHLHNCEICQCRPRPKKCKPLVCDKYCPFGYLKNKHGCETCRCKKCPDAPCSKICPMGFEQDNHGCRICKCRETMTSVVPPVKTGSCLSMDGRRHENEESWHDGCRECYCHNGKEMCGLITCPVPDCVNPTIYPGQCCPSCPDDSNAQNPELTDPSICHAPGGEYFVEGETWNIDTCTQCTCHSGRVLCETEVCPPLLCQNPTRSQDSCCPQCPDDSLQPSVPSNDSVPSYCKNDEGDIFLAAESWKPNVCTSCVCMDGIISCYSESCPPVTCERPVLRKGQCCPYCIEDTIPKKVVCHFNGKTYADEERWDIDSCTHCYCLQGQTLCSTVSCPPLPCVEPMNVESSCCPMCPEMYIPEPTNIPIEKTNHRGKNELDMPHWATPSENDIVHHHRDMGHLQANYREVERPRLTEEDSLHSVAWVAVPIMIALVILIVLFMINQKKQWIPVPCYRTPNKPTCLNNQLVYVDCKKGTMVQVDSSQRMLRMADPDSRYSGFYSMQKQNNLQADNFYQTV

Target ORF information:

RefSeq Version	XM_018261588.1
Organism	Xenopus laevis(African clawed frog)
Definition	Xenopus laevis cysteine rich transmembrane BMP regulator 1 (chordin-like) L homeolog (crim1.L), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018261588.1

ORF Insert Sequence:

ATGTACCTGC CGGGGAAGTT ACTGCGTCTG TGGCTCCTGC TGCTGCTGGT TTTGACGCTG 
CTGCCAGCGT CTGCCTGGGC TTTGGTCTGC TTGCCCTGCG ACGAATCCAA ATGCGAGGAA 
CCCAAGAGTT GCCCGGGGAG TATAGTTCAA GGCATCTGCG GCTGCTGCTT CATGTGCGCC 
AGGCAGAAGA ACGAGAGCTG CGGGGGAGTC TATGGGCTAC ACGGAGCCTG CGACAGGGGT 
CTGCGCTGTG TCATCCGCCC CCCGCTTAAT GGCGACTCCA TTACAGAATA TGAAGTGGGG 
GTTTGCGAAG ATGAAAACTG GGATGATGAC CAATTGCATG GCTTCGTCCC TTGCAATGAA 
AACTTTGTAA CCGGTTGCAA CATCATCAAT GGGAAATGTG AATGCGATAC CATACGGACC 
TGTAACACTC CCTTTGAGTT CCCCAGCCAG CATGCCTGCC TGACTGCGCT GAGACGGATT 
GAAGAAGAGA GACCAGACTG CTCATCAGCT CGATGTGAGG TACAGTTCTC TCCACGTTGT 
GCCGAGGACT CCGTTTTGAT AGAAGGCTAT GCCCCACCTG GTGAATGCTG CCCGCTGCCA 
AGCCGATGTG TATGCAACCC AGCCGGATGT CTAAGGAAAG TTTGCCAGCC TGGTTACTTA 
AATATTTTGG TATCCAAAGC ATCTGGCAAG CCTGGGGAAT GTTGTGACCT TTATGAATGC 
AAACCAGTCT TCAGTGTGGA CTGCAGTACT GTGGAGTGCC CTCCAGTTCA ACAGGCCATA 
TGCCCATTGG ATAGCTATGA GACACAAGTC CGCCTTACAG CTGACGGATG CTGCACGCTG 
CCTACAAGAT GTGAGTGTCT CTCGGGCCTC TGCACTTTCC CTGTATGTGA TGCTGGATCA 
ATCCCCCGCA TAGTCTCTCG AGGAGATGGC ACACCTGGAA AGTGCTGTGA CGTCTTTGAA 
TGTGTTAATG ATCCTGTGTA CCCAGTCCAT GACCCAGCAG GTTGCTACGC CAATGGACAA 
ATAAGAAGTC ACGGGGACCG CTGGAGAGAA GATGACTGCA CCTTCTGCCA GTGTATCAAT 
GGAGAGCCTC ACTGTGTTGC AACAGCTTGT GGCCAGAGTT GCTTGAAACC AGTTAAAGTG 
CCTGGAGAAT GTTGTCCTGT GTGTGAAGAG CCTACATATA TCACTATGGC TCCACCCATC 
TGCGACCTGC TGATCAACTG CACGTTGACT GAAAAGGACT GCAGCTATGG TTTCCGATTA 
GATCAAAATG GATGCCGCAC ATGCCAATGC AAAACAAGAG AGGAGCTCTG TACTGGTCTC 
ATATCAGGCT GTGCATTGGA CTGCCCTTTT GGATTTCAGA CTCATCTACA CAACTGTGAG 
ATCTGCCAGT GTCGACCTCG GCCCAAGAAG TGCAAACCTT TAGTGTGTGA CAAATACTGT 
CCGTTTGGCT ACCTGAAGAA CAAGCATGGC TGTGAAACAT GTCGCTGTAA GAAGTGCCCT 
GATGCCCCTT GCAGCAAAAT CTGCCCAATG GGATTTGAAC AGGACAATCA CGGATGCCGC 
ATCTGCAAAT GCAGGGAAAC AATGACATCT GTGGTACCCC CAGTCAAAAC AGGCTCATGC 
TTGTCAATGG ATGGTCGACG CCATGAAAAT GAAGAGAGTT GGCATGATGG ATGTCGTGAG 
TGCTATTGTC ACAATGGCAA AGAAATGTGT GGACTCATTA CATGTCCTGT TCCTGATTGT 
GTGAACCCCA CTATATACCC AGGACAATGC TGCCCTTCAT GTCCAGATGA TTCCAATGCA 
CAGAATCCAG AACTTACAGA CCCATCCATT TGCCATGCTC CTGGAGGGGA GTACTTTGTT 
GAAGGGGAAA CCTGGAATAT TGACACATGC ACCCAGTGCA CTTGCCATAG TGGGCGGGTG 
CTTTGTGAAA CTGAGGTCTG CCCACCTCTT CTTTGCCAGA ACCCAACACG TTCCCAGGAT 
TCTTGCTGTC CTCAATGCCC AGATGATTCC CTGCAGCCTT CTGTCCCAAG CAACGACAGT 
GTCCCCAGCT ACTGCAAAAA TGACGAGGGC GACATTTTCC TGGCTGCTGA GTCTTGGAAA 
CCTAATGTCT GTACAAGCTG CGTCTGCATG GACGGGATTA TCAGCTGCTA CTCTGAATCC 
TGCCCCCCTG TAACCTGTGA GAGACCGGTT TTAAGGAAGG GTCAGTGCTG TCCATATTGC 
ATAGAAGACA CAATTCCAAA GAAGGTGGTG TGCCATTTCA ATGGCAAAAC ATATGCGGAT 
GAGGAGCGGT GGGACATTGA CAGCTGCACA CATTGCTATT GCCTTCAAGG ACAGACCCTC 
TGCTCTACTG TGAGCTGCCC CCCTCTTCCC TGTGTGGAGC CAATGAATGT TGAAAGTAGC 
TGTTGTCCTA TGTGTCCAGA AATGTATATA CCGGAACCCA CAAATATCCC TATTGAGAAG 
ACAAACCACA GAGGAAAGAA TGAGCTAGAC ATGCCTCACT GGGCAACTCC TAGCGAGAAT 
GACATCGTTC ATCACCACAG AGACATGGGT CACCTTCAGG CAAACTACAG AGAAGTGGAA 
AGACCCCGTT TGACCGAAGA AGACTCTCTT CATTCTGTGG CATGGGTTGC GGTCCCTATC 
ATGATTGCGT TGGTTATCCT AATTGTTCTT TTTATGATTA ATCAGAAAAA ACAGTGGATA 
CCAGTACCCT GTTATAGAAC GCCTAACAAG CCGACCTGTC TGAACAATCA GCTTGTGTAT 
GTGGACTGCA AAAAAGGAAC CATGGTTCAA GTGGACAGCT CCCAGAGAAT GTTGAGAATG 
GCAGACCCGG ACTCAAGATA CAGCGGATTC TACAGTATGC AAAAACAAAA TAACCTCCAG 
GCAGATAATT TTTACCAAAC TGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

crim1.L ( NM_001170446.1 ) cDNA ORF clone, Xenopus laevis(African clawed frog) -> NP_001163917.1 Xenopus laevis cysteine rich transmembrane BMP regulator 1 (chordin-like) L homeolog (crim1.L), mRNA.

Price & Availability↑

CloneID	OAf05350
Clone ID Related Accession (Same CDS sequence)	NM_001170446.1
Accession Version	NM_001170446.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3090bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-10-28
Organism	Xenopus laevis(African clawed frog)
Product	cysteine rich transmembrane BMP regulator 1 (chordin-like) L homeolog precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from EU882845.1. ##Evidence-Data-START## Transcript exon combination :: EU882845.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00012418, SAMD00012419 [ECO:0000348] ##Evidence-Data-END##

ATGTACCTGC CGGGGAAGTT ACTGCGTCTG TGGCTCCTGC TGCTGCTGGT TTTGACGCTG 
CTGCCAGCGT CTGCCTGGGC TTTGGTCTGC TTGCCCTGCG ACGAATCCAA ATGCGAGGAA 
CCCAAGAGTT GCCCGGGGAG TATAGTTCAA GGCATCTGCG GCTGCTGCTT CATGTGCGCC 
AGGCAGAAGA ACGAGAGCTG CGGGGGAGTC TATGGGCTAC ACGGAGCCTG CGACAGGGGT 
CTGCGCTGTG TCATCCGCCC CCCGCTTAAT GGCGACTCCA TTACAGAATA TGAAGTGGGG 
GTTTGCGAAG ATGAAAACTG GGATGATGAC CAATTGCATG GCTTCGTCCC TTGCAATGAA 
AACTTTGTAA CCGGTTGCAA CATCATCAAT GGGAAATGTG AATGCGATAC CATACGGACC 
TGTAACACTC CCTTTGAGTT CCCCAGCCAG CATGCCTGCC TGACTGCGCT GAGACGGATT 
GAAGAAGAGA GACCAGACTG CTCATCAGCT CGATGTGAGG TACAGTTCTC TCCACGTTGT 
GCCGAGGACT CCGTTTTGAT AGAAGGCTAT GCCCCACCTG GTGAATGCTG CCCGCTGCCA 
AGCCGATGTG TATGCAACCC AGCCGGATGT CTAAGGAAAG TTTGCCAGCC TGGTTACTTA 
AATATTTTGG TATCCAAAGC ATCTGGCAAG CCTGGGGAAT GTTGTGACCT TTATGAATGC 
AAACCAGTCT TCAGTGTGGA CTGCAGTACT GTGGAGTGCC CTCCAGTTCA ACAGGCCATA 
TGCCCATTGG ATAGCTATGA GACACAAGTC CGCCTTACAG CTGACGGATG CTGCACGCTG 
CCTACAAGAT GTGAGTGTCT CTCGGGCCTC TGCACTTTCC CTGTATGTGA TGCTGGATCA 
ATCCCCCGCA TAGTCTCTCG AGGAGATGGC ACACCTGGAA AGTGCTGTGA CGTCTTTGAA 
TGTGTTAATG AAACCAAGCC TGCGTGCATT TTTAATAACG TGGAGTACTA TGATGGGGAC 
ATGTTCCGTA TGGATGCGTG TCGATTTTGC CGCTGTCAGG GTGGCGTTTC CATCTGTTTC 
TCAGCCCAGT GTGGAGAGCT TCACTGCGAT CGCTACTATG TGCCAGAAGG GGAGTGCTGT 
CCAGTGTGTG AAGATCCTGT GTACCCAGTC CATGACCCAG CAGGTTGCTA CGCCAATGGA 
CAAATAAGAA GTCACGGGGA CCGCTGGAGA GAAGATGACT GCACCTTCTG CCAGTGTATC 
AATGGAGAGC CTCACTGTGT TGCAACAGCT TGTGGCCAGA GTTGCTTGAA ACCAGTTAAA 
GTGCCTGGAG AATGTTGTCC TGTGTGTGAA GAGCCTACAT ATATCACTAT GGCTCCACCC 
ATCTGCGACC TGCTGATCAA CTGCACGTTG ACTGAAAAGG ACTGCAGCTA TGGTTTCCGA 
TTAGATCAAA ATGGATGCCG CACATGCCAA TGCAAAACAA GAGAGGAGCT CTGTACTGGT 
CTCATATCAG GCTGTGCATT GGACTGCCCT TTTGGATTTC AGACTCATCT ACACAACTGT 
GAGATCTGCC AGTGTCGACC TCGGCCCAAG AAGTGCAAAC CTTTAGTGTG TGACAAATAC 
TGTCCGTTTG GCTACCTGAA GAACAAGCAT GGCTGTGAAA CATGTCGCTG TAAGAAGTGC 
CCTGATGCCC CTTGCAGCAA AATCTGCCCA ATGGGATTTG AACAGGACAA TCACGGATGC 
CGCATCTGCA AATGCAGGGA AACAATGACA TCTGTGGTAC CCCCAGTCAA AACAGGCTCA 
TGCTTGTCAA TGGATGGTCG ACGCCATGAA AATGAAGAGA GTTGGCATGA TGGATGTCGT 
GAGTGCTATT GTCACAATGG CAAAGAAATG TGTGGACTCA TTACATGTCC TGTTCCTGAT 
TGTGTGAACC CCACTATATA CCCAGGACAA TGCTGCCCTT CATGTCCAGA TGATTCCAAT 
GCACAGAATC CAGAACTTAC AGACCCATCC ATTTGCCATG CTCCTGGAGG GGAGTACTTT 
GTTGAAGGGG AAACCTGGAA TATTGACACA TGCACCCAGT GCACTTGCCA TAGTGGGCGG 
GTGCTTTGTG AAACTGAGGT CTGCCCACCT CTTCTTTGCC AGAACCCAAC ACGTTCCCAG 
GATTCTTGCT GTCCTCAATG CCCAGATGAT TCCCTGCAGC CTTCTGTCCC AAGCAACGAC 
AGTGTCCCCA GCTACTGCAA AAATGACGAG GGCGACATTT TCCTGGCTGC TGAGTCTTGG 
AAACCTAATG TCTGTACAAG CTGCGTCTGC ATGGACGGGA TTATCAGCTG CTACTCTGAA 
TCCTGCCCCC CTGTAACCTG TGAGAGACCG GTTTTAAGGA AGGGTCAGTG CTGTCCATAT 
TGCATAGAAG ACACAATTCC AAAGAAGGTG GTGTGCCATT TCAATGGCAA AACATATGCG 
GATGAGGAGC GGTGGGACAT TGACAGCTGC ACACATTGCT ATTGCCTTCA AGGACAGACC 
CTCTGCTCTA CTGTGAGCTG CCCCCCTCTT CCCTGTGTGG AGCCAATGAA TGTTGAAAGT 
AGCTGTTGTC CTATGTGTCC AGAAATGTAT ATACCGGAAC CCACAAATAT CCCTATTGAG 
AAGACAAACC ACAGAGGAAA GAATGAGCTA GACATGCCTC ACTGGGCAAC TCCTAGCGAG 
AATGACATCG TTCATCACCA CAGAGACATG GGTCACCTTC AGGCAAACTA CAGAGAAGTG 
GAAAGACCCC GTTTGACCGA AGAAGACTCT CTTCATTCTG TGGCATGGGT TGCGGTCCCT 
ATCATGATTG CGTTGGTTAT CCTAATTGTT CTTTTTATGA TTAATCAGAA AAAACAGTGG 
ATACCAGTAC CCTGTTATAG AACGCCTAAC AAGCCGACCT GTCTGAACAA TCAGCTTGTG 
TATGTGGACT GCAAAAAAGG AACCATGGTT CAAGTGGACA GCTCCCAGAG AATGTTGAGA 
ATGGCAGACC CGGACTCAAG ATACAGCGGA TTCTACAGTA TGCAAAAACA AAATAACCTC 
CAGGCAGATA ATTTTTACCA AACTGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001163917.1
CDS	1..3090
Translation	MYLPGKLLRLWLLLLLVLTLLPASAWALVCLPCDESKCEEPKSCPGSIVQGICGCCFMCARQKNESCGGVYGLHGACDRGLRCVIRPPLNGDSITEYEVGVCEDENWDDDQLHGFVPCNENFVTGCNIINGKCECDTIRTCNTPFEFPSQHACLTALRRIEEERPDCSSARCEVQFSPRCAEDSVLIEGYAPPGECCPLPSRCVCNPAGCLRKVCQPGYLNILVSKASGKPGECCDLYECKPVFSVDCSTVECPPVQQAICPLDSYETQVRLTADGCCTLPTRCECLSGLCTFPVCDAGSIPRIVSRGDGTPGKCCDVFECVNETKPACIFNNVEYYDGDMFRMDACRFCRCQGGVSICFSAQCGELHCDRYYVPEGECCPVCEDPVYPVHDPAGCYANGQIRSHGDRWREDDCTFCQCINGEPHCVATACGQSCLKPVKVPGECCPVCEEPTYITMAPPICDLLINCTLTEKDCSYGFRLDQNGCRTCQCKTREELCTGLISGCALDCPFGFQTHLHNCEICQCRPRPKKCKPLVCDKYCPFGYLKNKHGCETCRCKKCPDAPCSKICPMGFEQDNHGCRICKCRETMTSVVPPVKTGSCLSMDGRRHENEESWHDGCRECYCHNGKEMCGLITCPVPDCVNPTIYPGQCCPSCPDDSNAQNPELTDPSICHAPGGEYFVEGETWNIDTCTQCTCHSGRVLCETEVCPPLLCQNPTRSQDSCCPQCPDDSLQPSVPSNDSVPSYCKNDEGDIFLAAESWKPNVCTSCVCMDGIISCYSESCPPVTCERPVLRKGQCCPYCIEDTIPKKVVCHFNGKTYADEERWDIDSCTHCYCLQGQTLCSTVSCPPLPCVEPMNVESSCCPMCPEMYIPEPTNIPIEKTNHRGKNELDMPHWATPSENDIVHHHRDMGHLQANYREVERPRLTEEDSLHSVAWVAVPIMIALVILIVLFMINQKKQWIPVPCYRTPNKPTCLNNQLVYVDCKKGTMVQVDSSQRMLRMADPDSRYSGFYSMQKQNNLQADNFYQTV

Target ORF information:

RefSeq Version	NM_001170446.1
Organism	Xenopus laevis(African clawed frog)
Definition	Xenopus laevis cysteine rich transmembrane BMP regulator 1 (chordin-like) L homeolog (crim1.L), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001170446.1

ORF Insert Sequence:

ATGTACCTGC CGGGGAAGTT ACTGCGTCTG TGGCTCCTGC TGCTGCTGGT TTTGACGCTG 
CTGCCAGCGT CTGCCTGGGC TTTGGTCTGC TTGCCCTGCG ACGAATCCAA ATGCGAGGAA 
CCCAAGAGTT GCCCGGGGAG TATAGTTCAA GGCATCTGCG GCTGCTGCTT CATGTGCGCC 
AGGCAGAAGA ACGAGAGCTG CGGGGGAGTC TATGGGCTAC ACGGAGCCTG CGACAGGGGT 
CTGCGCTGTG TCATCCGCCC CCCGCTTAAT GGCGACTCCA TTACAGAATA TGAAGTGGGG 
GTTTGCGAAG ATGAAAACTG GGATGATGAC CAATTGCATG GCTTCGTCCC TTGCAATGAA 
AACTTTGTAA CCGGTTGCAA CATCATCAAT GGGAAATGTG AATGCGATAC CATACGGACC 
TGTAACACTC CCTTTGAGTT CCCCAGCCAG CATGCCTGCC TGACTGCGCT GAGACGGATT 
GAAGAAGAGA GACCAGACTG CTCATCAGCT CGATGTGAGG TACAGTTCTC TCCACGTTGT 
GCCGAGGACT CCGTTTTGAT AGAAGGCTAT GCCCCACCTG GTGAATGCTG CCCGCTGCCA 
AGCCGATGTG TATGCAACCC AGCCGGATGT CTAAGGAAAG TTTGCCAGCC TGGTTACTTA 
AATATTTTGG TATCCAAAGC ATCTGGCAAG CCTGGGGAAT GTTGTGACCT TTATGAATGC 
AAACCAGTCT TCAGTGTGGA CTGCAGTACT GTGGAGTGCC CTCCAGTTCA ACAGGCCATA 
TGCCCATTGG ATAGCTATGA GACACAAGTC CGCCTTACAG CTGACGGATG CTGCACGCTG 
CCTACAAGAT GTGAGTGTCT CTCGGGCCTC TGCACTTTCC CTGTATGTGA TGCTGGATCA 
ATCCCCCGCA TAGTCTCTCG AGGAGATGGC ACACCTGGAA AGTGCTGTGA CGTCTTTGAA 
TGTGTTAATG AAACCAAGCC TGCGTGCATT TTTAATAACG TGGAGTACTA TGATGGGGAC 
ATGTTCCGTA TGGATGCGTG TCGATTTTGC CGCTGTCAGG GTGGCGTTTC CATCTGTTTC 
TCAGCCCAGT GTGGAGAGCT TCACTGCGAT CGCTACTATG TGCCAGAAGG GGAGTGCTGT 
CCAGTGTGTG AAGATCCTGT GTACCCAGTC CATGACCCAG CAGGTTGCTA CGCCAATGGA 
CAAATAAGAA GTCACGGGGA CCGCTGGAGA GAAGATGACT GCACCTTCTG CCAGTGTATC 
AATGGAGAGC CTCACTGTGT TGCAACAGCT TGTGGCCAGA GTTGCTTGAA ACCAGTTAAA 
GTGCCTGGAG AATGTTGTCC TGTGTGTGAA GAGCCTACAT ATATCACTAT GGCTCCACCC 
ATCTGCGACC TGCTGATCAA CTGCACGTTG ACTGAAAAGG ACTGCAGCTA TGGTTTCCGA 
TTAGATCAAA ATGGATGCCG CACATGCCAA TGCAAAACAA GAGAGGAGCT CTGTACTGGT 
CTCATATCAG GCTGTGCATT GGACTGCCCT TTTGGATTTC AGACTCATCT ACACAACTGT 
GAGATCTGCC AGTGTCGACC TCGGCCCAAG AAGTGCAAAC CTTTAGTGTG TGACAAATAC 
TGTCCGTTTG GCTACCTGAA GAACAAGCAT GGCTGTGAAA CATGTCGCTG TAAGAAGTGC 
CCTGATGCCC CTTGCAGCAA AATCTGCCCA ATGGGATTTG AACAGGACAA TCACGGATGC 
CGCATCTGCA AATGCAGGGA AACAATGACA TCTGTGGTAC CCCCAGTCAA AACAGGCTCA 
TGCTTGTCAA TGGATGGTCG ACGCCATGAA AATGAAGAGA GTTGGCATGA TGGATGTCGT 
GAGTGCTATT GTCACAATGG CAAAGAAATG TGTGGACTCA TTACATGTCC TGTTCCTGAT 
TGTGTGAACC CCACTATATA CCCAGGACAA TGCTGCCCTT CATGTCCAGA TGATTCCAAT 
GCACAGAATC CAGAACTTAC AGACCCATCC ATTTGCCATG CTCCTGGAGG GGAGTACTTT 
GTTGAAGGGG AAACCTGGAA TATTGACACA TGCACCCAGT GCACTTGCCA TAGTGGGCGG 
GTGCTTTGTG AAACTGAGGT CTGCCCACCT CTTCTTTGCC AGAACCCAAC ACGTTCCCAG 
GATTCTTGCT GTCCTCAATG CCCAGATGAT TCCCTGCAGC CTTCTGTCCC AAGCAACGAC 
AGTGTCCCCA GCTACTGCAA AAATGACGAG GGCGACATTT TCCTGGCTGC TGAGTCTTGG 
AAACCTAATG TCTGTACAAG CTGCGTCTGC ATGGACGGGA TTATCAGCTG CTACTCTGAA 
TCCTGCCCCC CTGTAACCTG TGAGAGACCG GTTTTAAGGA AGGGTCAGTG CTGTCCATAT 
TGCATAGAAG ACACAATTCC AAAGAAGGTG GTGTGCCATT TCAATGGCAA AACATATGCG 
GATGAGGAGC GGTGGGACAT TGACAGCTGC ACACATTGCT ATTGCCTTCA AGGACAGACC 
CTCTGCTCTA CTGTGAGCTG CCCCCCTCTT CCCTGTGTGG AGCCAATGAA TGTTGAAAGT 
AGCTGTTGTC CTATGTGTCC AGAAATGTAT ATACCGGAAC CCACAAATAT CCCTATTGAG 
AAGACAAACC ACAGAGGAAA GAATGAGCTA GACATGCCTC ACTGGGCAAC TCCTAGCGAG 
AATGACATCG TTCATCACCA CAGAGACATG GGTCACCTTC AGGCAAACTA CAGAGAAGTG 
GAAAGACCCC GTTTGACCGA AGAAGACTCT CTTCATTCTG TGGCATGGGT TGCGGTCCCT 
ATCATGATTG CGTTGGTTAT CCTAATTGTT CTTTTTATGA TTAATCAGAA AAAACAGTGG 
ATACCAGTAC CCTGTTATAG AACGCCTAAC AAGCCGACCT GTCTGAACAA TCAGCTTGTG 
TATGTGGACT GCAAAAAAGG AACCATGGTT CAAGTGGACA GCTCCCAGAG AATGTTGAGA 
ATGGCAGACC CGGACTCAAG ATACAGCGGA TTCTACAGTA TGCAAAAACA AAATAACCTC 
CAGGCAGATA ATTTTTACCA AACTGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

CRIM1 complexes with ?-catenin and cadherins, stabilizes cell-cell junctions and is critical for neural morphogenesis.
PloS one7(3)e32635(2012)
Ponferrada VG,Fan J,Vallance JE,Hu S,Mamedova A,Rankin SA,Kofron M,Zorn AM,Hegde RS,Lang RA

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crim1.L cDNA ORF clone, Xenopus laevis(African clawed frog)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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