NPHS1 cDNA ORF clone, Tursiops truncatus(common bottlenose dolphin)

Gene
Clones
gRNAs

Summary

Gene Symbol	NPHS1
Entrez Gene ID	101339565
Full Name	NPHS1, nephrin
Gene Type	protein-coding
Organism	Tursiops truncatus(common bottlenose dolphin)

mRNA and Protein(s)

mRNA	Protein	Name
XM_019941052.1	XP_019796611.1	nephrin

Pathways from BioSystems
Gene Ontology

The following NPHS1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NPHS1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OTu47139	XM_019941052.1 Latest version!	Tursiops truncatus NPHS1, nephrin (NPHS1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NPHS1 ( XM_019941052.1 ) cDNA ORF clone, Tursiops truncatus(common bottlenose dolphin) -> XP_019796611.1 Tursiops truncatus NPHS1, nephrin (NPHS1), mRNA.

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CloneID	OTu47139
Clone ID Related Accession (Same CDS sequence)	XM_019941052.1
Accession Version	XM_019941052.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2325bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-01-11
Organism	Tursiops truncatus(common bottlenose dolphin)
Product	nephrin
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017843553.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Tursiops truncatus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 4% of CDS bases ##RefSeq-Attributes-END##

ATGGAAAACC CAGAGAAAGA CAGAGGCAGA CGCAGGAGGC GGGCAGTGGT CCCTGTGGGA 
GCCACAGGCA GAGGACCTGG GCTGGCTCTG AGGCTCCCCA TCTGGACACC GCTGCTCCTC 
TTGGGGCTGC TGATGACAGG CCTGGCCCAG GTGCCAGTCC CTGCCTCAGC CCCGCGAGGC 
TTCTGGGCTC TGCCTGAAAA CTTGACGGTG GTGGAGGGGG CTGCCGCTGA GCTGTGGTGT 
GGGGTCAGCG CCCCTGGCAG CACAGTCCAA TGGGCCAAAG ATGGGCTGCT CCTAGGCCCT 
GATCCTAGGA TCCCCAGTTT CCCGCGGTAT CGCCTGCAAG GGGACCCTAC GAAAGGTGAA 
TTCCACCTGC ACATTGAAGC ATGTGACCTC AGCGACGACG CAGGGTATGA GTGCCAGGTC 
AGCCGCTCAG AAACAGGTCC TGAGCTCGTG TCGCCCAGAG TGATCCTCTC CATCCTGGTG 
CCCCCCAAAG TGCTTCAGCT GACCCCCGAG GCAGGGAGCA CGGTCACGTG GGTAGCTGGG 
CAGGAGTACT TGGTCAGCTG TGTGTCTGGG GATGCAAAGC CAGCACCTGA CATCACCTTC 
CTCCTGAGAT GGTGGCTGGG CTGGCGGCAG CTGCTGTCCA CAGAGGAGAC GGTCATGGAT 
GGCCTGCATG GTGGCCACAT CTCCATGTCT AACCTGACAT TCCTGGCGCG ACGGGAGGAC 
AATGGTCTGA CCCTCACATG TGAGGCCTTT AGTGAGGCCT TCACCAAGGA GACCTTCAAG 
AAGTCACTCG CCCTGAATGT GAAATATCCT GCCCAGAAGC TATGGATTGA GGGCCCCCCA 
GAGGGGCAGC GTCTCCGGGC TGGGACCCGG GTGAGGCTGG TATGTTTGGC CATCGGAGGC 
AATCCAGACC CTTCCCTCAC CTGGTACAAG GGAGAAGAGG AGGAGGACCA GAGCCTGGAT 
GATATGGAGA AGATATCAAA GGGGTCCACA GGGCGTCTTC GGATTCACCA TGCCAAGTTG 
ACCCAGGCTG GTGCTTACCA GTGCATTGTG GACAATGGGG TGGCACCTCC AGCCAGAGGG 
CTGGTCCGTC TTGTTGTTAG ATTTGCCCCC CACGTGGAGC ATCCCTCTCC TCTAACTAAA 
GTGGCTGCAG CTGGAGACAG CACTAGTTCT GCCACCCTCC ACTGTCGTGC CCGAGGTGTC 
CCCAATATCG TCTTCACTTG GACCAAAAAT GGGGTCCCTC TGGATCTCCA GGATCCCAGG 
TACACAGAGC ACACATACCA CCAGAGTGGT GTCCACAGCA GTCTCCTGAC CATTGCCAAC 
GTGTCCGCAG CCCAGGACTA TGCCCTCTTC ACGTGCACAG CCACCAACCC CCTCGGCTCG 
GACCACACCG ATATTCAACT CGTCAGCATC AGCCGCCCTG ATCCGCCATT GGGATTGAAG 
GTCGTGAGCA TGACCCCATA CTCGGTGGGG CTGGAGTGGA AGCCTGGCTT TGATGGGGGC 
TTGCCACAAA GTTTCCAAGT CAGGTATGAG GCTCTGGGGA CTCCAGGGTT TCTCTACGTG 
AATGTCCTAC CACCCCAGGC CACCGCCTTC ACACTGACTG GGCTGCAGCC TTCTACACGA 
TACAGGGTCT GGTTGCTGGC CAGCAATGCC CTGGGCGACA GTGGAATAGC TGACAAAGGG 
TCCCAGATCT CAGTCACCAC TCCAGGTCTA GACCAACCTT CTGGAGAACC TGACTACCAG 
CTGCCCACAG AGCAGCCCGC AGGACCCTCA AAGCTGCCCC TGCTGCCTGT GCTGTTTGCT 
GTTGGGGGTC TTCTGCTGCT TTCTAACGCC TCCTGTGTCG GGGGATTCCT CTGGCAGCGG 
AGACTGAAGC GTCTTGCTGA GGGCATCTCA GAGAAGACAG AGGCAGGGTC GGAGGAAGAC 
CGAGTCAGGA ATGAATACGA GGAGAGCCAG TGGACTGGAG ACCGTGACAC TCGAAGCTCC 
ATGGTTAGCA CAACAGATGT AGAGCCTTAT TACCACTCCA TGAGGGACTT CAGCCCCCAG 
CTTCCCCCCA TGCTGGAGGA GGTGTCTTAT CCCCGAGGTG TCACAGGTCT TAAGGAGGAG 
AATATGGCTT TTCCTGGGCA TGTGTATGAT GAGCTGGAAA GACTGTACGC CCCGTCTGGG 
GCCTGGGGAC CCCTCTATGA TGAAGTACCA ATGGGCTCCT GTGACCTCTA CTGGCCTGAA 
GACAGGTATG AAGATGCAAG AGGAATCTAT GATCAGGTGG CAGGAGATTT GGACCCTATG 
GAACCTGATG CTCTACCCTT TGAGCTGAGG GGACAGCTTG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019796611.1
CDS	1..2325
Translation	MENPEKDRGRRRRRAVVPVGATGRGPGLALRLPIWTPLLLLGLLMTGLAQVPVPASAPRGFWALPENLTVVEGAAAELWCGVSAPGSTVQWAKDGLLLGPDPRIPSFPRYRLQGDPTKGEFHLHIEACDLSDDAGYECQVSRSETGPELVSPRVILSILVPPKVLQLTPEAGSTVTWVAGQEYLVSCVSGDAKPAPDITFLLRWWLGWRQLLSTEETVMDGLHGGHISMSNLTFLARREDNGLTLTCEAFSEAFTKETFKKSLALNVKYPAQKLWIEGPPEGQRLRAGTRVRLVCLAIGGNPDPSLTWYKGEEEEDQSLDDMEKISKGSTGRLRIHHAKLTQAGAYQCIVDNGVAPPARGLVRLVVRFAPHVEHPSPLTKVAAAGDSTSSATLHCRARGVPNIVFTWTKNGVPLDLQDPRYTEHTYHQSGVHSSLLTIANVSAAQDYALFTCTATNPLGSDHTDIQLVSISRPDPPLGLKVVSMTPYSVGLEWKPGFDGGLPQSFQVRYEALGTPGFLYVNVLPPQATAFTLTGLQPSTRYRVWLLASNALGDSGIADKGSQISVTTPGLDQPSGEPDYQLPTEQPAGPSKLPLLPVLFAVGGLLLLSNASCVGGFLWQRRLKRLAEGISEKTEAGSEEDRVRNEYEESQWTGDRDTRSSMVSTTDVEPYYHSMRDFSPQLPPMLEEVSYPRGVTGLKEENMAFPGHVYDELERLYAPSGAWGPLYDEVPMGSCDLYWPEDRYEDARGIYDQVAGDLDPMEPDALPFELRGQLV

Target ORF information:

RefSeq Version	XM_019941052.1
Organism	Tursiops truncatus(common bottlenose dolphin)
Definition	Tursiops truncatus NPHS1, nephrin (NPHS1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019941052.1

ORF Insert Sequence:

ATGGAAAACC CAGAGAAAGA CAGAGGCAGA CGCAGGAGGC GGGCAGTGGT CCCTGTGGGA 
GCCACAGGCA GAGGACCTGG GCTGGCTCTG AGGCTCCCCA TCTGGACACC GCTGCTCCTC 
TTGGGGCTGC TGATGACAGG CCTGGCCCAG GTGCCAGTCC CTGCCTCAGC CCCGCGAGGC 
TTCTGGGCTC TGCCTGAAAA CTTGACGGTG GTGGAGGGGG CTGCCGCTGA GCTGTGGTGT 
GGGGTCAGCG CCCCTGGCAG CACAGTCCAA TGGGCCAAAG ATGGGCTGCT CCTAGGCCCT 
GATCCTAGGA TCCCCAGTTT CCCGCGGTAT CGCCTGCAAG GGGACCCTAC GAAAGGTGAA 
TTCCACCTGC ACATTGAAGC ATGTGACCTC AGCGACGACG CAGGGTATGA GTGCCAGGTC 
AGCCGCTCAG AAACAGGTCC TGAGCTCGTG TCGCCCAGAG TGATCCTCTC CATCCTGGTG 
CCCCCCAAAG TGCTTCAGCT GACCCCCGAG GCAGGGAGCA CGGTCACGTG GGTAGCTGGG 
CAGGAGTACT TGGTCAGCTG TGTGTCTGGG GATGCAAAGC CAGCACCTGA CATCACCTTC 
CTCCTGAGAT GGTGGCTGGG CTGGCGGCAG CTGCTGTCCA CAGAGGAGAC GGTCATGGAT 
GGCCTGCATG GTGGCCACAT CTCCATGTCT AACCTGACAT TCCTGGCGCG ACGGGAGGAC 
AATGGTCTGA CCCTCACATG TGAGGCCTTT AGTGAGGCCT TCACCAAGGA GACCTTCAAG 
AAGTCACTCG CCCTGAATGT GAAATATCCT GCCCAGAAGC TATGGATTGA GGGCCCCCCA 
GAGGGGCAGC GTCTCCGGGC TGGGACCCGG GTGAGGCTGG TATGTTTGGC CATCGGAGGC 
AATCCAGACC CTTCCCTCAC CTGGTACAAG GGAGAAGAGG AGGAGGACCA GAGCCTGGAT 
GATATGGAGA AGATATCAAA GGGGTCCACA GGGCGTCTTC GGATTCACCA TGCCAAGTTG 
ACCCAGGCTG GTGCTTACCA GTGCATTGTG GACAATGGGG TGGCACCTCC AGCCAGAGGG 
CTGGTCCGTC TTGTTGTTAG ATTTGCCCCC CACGTGGAGC ATCCCTCTCC TCTAACTAAA 
GTGGCTGCAG CTGGAGACAG CACTAGTTCT GCCACCCTCC ACTGTCGTGC CCGAGGTGTC 
CCCAATATCG TCTTCACTTG GACCAAAAAT GGGGTCCCTC TGGATCTCCA GGATCCCAGG 
TACACAGAGC ACACATACCA CCAGAGTGGT GTCCACAGCA GTCTCCTGAC CATTGCCAAC 
GTGTCCGCAG CCCAGGACTA TGCCCTCTTC ACGTGCACAG CCACCAACCC CCTCGGCTCG 
GACCACACCG ATATTCAACT CGTCAGCATC AGCCGCCCTG ATCCGCCATT GGGATTGAAG 
GTCGTGAGCA TGACCCCATA CTCGGTGGGG CTGGAGTGGA AGCCTGGCTT TGATGGGGGC 
TTGCCACAAA GTTTCCAAGT CAGGTATGAG GCTCTGGGGA CTCCAGGGTT TCTCTACGTG 
AATGTCCTAC CACCCCAGGC CACCGCCTTC ACACTGACTG GGCTGCAGCC TTCTACACGA 
TACAGGGTCT GGTTGCTGGC CAGCAATGCC CTGGGCGACA GTGGAATAGC TGACAAAGGG 
TCCCAGATCT CAGTCACCAC TCCAGGTCTA GACCAACCTT CTGGAGAACC TGACTACCAG 
CTGCCCACAG AGCAGCCCGC AGGACCCTCA AAGCTGCCCC TGCTGCCTGT GCTGTTTGCT 
GTTGGGGGTC TTCTGCTGCT TTCTAACGCC TCCTGTGTCG GGGGATTCCT CTGGCAGCGG 
AGACTGAAGC GTCTTGCTGA GGGCATCTCA GAGAAGACAG AGGCAGGGTC GGAGGAAGAC 
CGAGTCAGGA ATGAATACGA GGAGAGCCAG TGGACTGGAG ACCGTGACAC TCGAAGCTCC 
ATGGTTAGCA CAACAGATGT AGAGCCTTAT TACCACTCCA TGAGGGACTT CAGCCCCCAG 
CTTCCCCCCA TGCTGGAGGA GGTGTCTTAT CCCCGAGGTG TCACAGGTCT TAAGGAGGAG 
AATATGGCTT TTCCTGGGCA TGTGTATGAT GAGCTGGAAA GACTGTACGC CCCGTCTGGG 
GCCTGGGGAC CCCTCTATGA TGAAGTACCA ATGGGCTCCT GTGACCTCTA CTGGCCTGAA 
GACAGGTATG AAGATGCAAG AGGAATCTAT GATCAGGTGG CAGGAGATTT GGACCCTATG 
GAACCTGATG CTCTACCCTT TGAGCTGAGG GGACAGCTTG TGTGA