BLMH cDNA ORF clone, Thamnophis sirtalis

Gene
Clones
gRNAs

Summary

Gene Symbol	BLMH
Entrez Gene ID	106550437
Full Name	bleomycin hydrolase
Gene Type	protein-coding
Organism	Thamnophis sirtalis

mRNA and Protein(s)

mRNA	Protein	Name
XM_014068350.1	XP_013923825.1	bleomycin hydrolase

Pathways from BioSystems
Gene Ontology

The following BLMH gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the BLMH cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OTh26077	XM_014068350.1 Latest version!	Thamnophis sirtalis bleomycin hydrolase (BLMH), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$104.30 $149.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

BLMH ( XM_014068350.1 ) cDNA ORF clone, Thamnophis sirtalis -> XP_013923825.1 Thamnophis sirtalis bleomycin hydrolase (BLMH), mRNA.

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CloneID	OTh26077
Clone ID Related Accession (Same CDS sequence)	XM_014068350.1
Accession Version	XM_014068350.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 495bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-10
Organism	Thamnophis sirtalis
Product	bleomycin hydrolase
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_013659187.1) and transcript sequence (GDKU01023367.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Thamnophis sirtalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 70 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGGTGGGGG GCAGGAAGAC GCTTTACAAC AACCAGCCGG TGGAGCTATT GAAAAAGATG 
GTGGCCGCGT CCATTCAGGA TGGAGAGGCT GTGTGGTTCG GCTGCGACGT TGGGAAATGT 
TTTAATAGCA AGCTGGGCAT CAACGATTTG AAGATATATA ACCATGAGTT GATGTTCGGG 
GTGTCGGTGA AGAACATGAA GAAGGACGAG CGGCTGATTT TCGGGGATTC CATGATGACG 
CACGCCATGG TTATCACCGC CTTCACGAAG AAAGAAGAAC CCGACGAGGG CTACGAGAAG 
TGGCGGGTGG AGAATTCGTG GGGTGAAGAC CGTGGGAATA AAGGTTACCT ACTCATGACG 
GACGACTGGT TCTCGGAGTT CGTGTACGAA GTGGTCGTCG ACCGGAAACA CGTCCCGGAG 
GAGGTCCTGG AAGTCTTGCA ACAGGAGCCC ACTTCCCTGC CCGCCTGGGA TCCCATGGGG 
GCTCTGGCCC ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013923825.1
CDS	70..564
Translation	MVGGRKTLYNNQPVELLKKMVAASIQDGEAVWFGCDVGKCFNSKLGINDLKIYNHELMFGVSVKNMKKDERLIFGDSMMTHAMVITAFTKKEEPDEGYEKWRVENSWGEDRGNKGYLLMTDDWFSEFVYEVVVDRKHVPEEVLEVLQQEPTSLPAWDPMGALAH

Target ORF information:

RefSeq Version	XM_014068350.1
Organism	Thamnophis sirtalis
Definition	Thamnophis sirtalis bleomycin hydrolase (BLMH), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014068350.1

ORF Insert Sequence:

ATGGTGGGGG GCAGGAAGAC GCTTTACAAC AACCAGCCGG TGGAGCTATT GAAAAAGATG 
GTGGCCGCGT CCATTCAGGA TGGAGAGGCT GTGTGGTTCG GCTGCGACGT TGGGAAATGT 
TTTAATAGCA AGCTGGGCAT CAACGATTTG AAGATATATA ACCATGAGTT GATGTTCGGG 
GTGTCGGTGA AGAACATGAA GAAGGACGAG CGGCTGATTT TCGGGGATTC CATGATGACG 
CACGCCATGG TTATCACCGC CTTCACGAAG AAAGAAGAAC CCGACGAGGG CTACGAGAAG 
TGGCGGGTGG AGAATTCGTG GGGTGAAGAC CGTGGGAATA AAGGTTACCT ACTCATGACG 
GACGACTGGT TCTCGGAGTT CGTGTACGAA GTGGTCGTCG ACCGGAAACA CGTCCCGGAG 
GAGGTCCTGG AAGTCTTGCA ACAGGAGCCC ACTTCCCTGC CCGCCTGGGA TCCCATGGGG 
GCTCTGGCCC ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.