ACTA1 cDNA ORF clone, Thamnophis sirtalis

Gene
Clones
gRNAs

Summary

Gene Symbol	ACTA1
Entrez Gene ID	106543751
Full Name	actin, alpha 1, skeletal muscle
Gene Type	protein-coding
Organism	Thamnophis sirtalis

mRNA and Protein(s)

mRNA	Protein	Name
XM_014059828.1	XP_013915303.1	actin, alpha skeletal muscle

Pathways from BioSystems
Gene Ontology

The following ACTA1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ACTA1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OTh19626	XM_014059828.1 Latest version!	Thamnophis sirtalis actin, alpha 1, skeletal muscle (ACTA1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ACTA1 ( XM_014059828.1 ) cDNA ORF clone, Thamnophis sirtalis -> XP_013915303.1 Thamnophis sirtalis actin, alpha 1, skeletal muscle (ACTA1), mRNA.

Price & Availability↑

CloneID	OTh19626
Clone ID Related Accession (Same CDS sequence)	XM_014059828.1
Accession Version	XM_014059828.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1134bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-10
Organism	Thamnophis sirtalis
Product	actin, alpha skeletal muscle
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_013658250.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Thamnophis sirtalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTGATG AGGATGAGAC CACCGCACTT GTGTGCGACA ACGGCTCTGG CTTGGTGAAA 
GCTGGCTTTG CCGGGGATGA CGCCCCTAGG GCTGTCTTTC CCTCCATTGT TGGCCGCCCA 
CGTCATCAGG GTGTCATGGT GGGTATGGGT CAAAAAGACT CCTACGTAGG GGATGAAGCC 
CAGAGCAAAA GAGGTATCCT GACCCTGAAG TACCCCATTG AACATGGCAT CATCACCAAC 
TGGGATGACA TGGAGAAGAT TTGGCATCAC ACTTTCTACA ATGAGCTCCG TGTTGCCCCA 
GAGGAGCACC CCACCCTGCT CACTGAAGCC CCTCTTAACC CCAAGGCCAA TCGTGAAAAG 
ATGACCCAGA TCATGTTTGA GACCTTCAAC GTCCCTGCCA TGTATGTGGC CATCCAGGCT 
GTGCTGTCCC TCTATGCCTC TGGCCGTACC ACCGGTATTG TGCTGGACTC TGGCGATGGT 
GTCACCCACA ATGTACCCAT CTATGAGGGT TATGCCCTGC CCCATGCCAT CATGCGTTTG 
GACTTGGCTG GTCGGGATCT CACTGACTAC CTGATGAAGA TCCTGACTGA GAGAGGCTAC 
TCTTTTGTCA CCACTGCTGA ACGTGAGATT GTCCGTGACA TCAAGGAAAA GCTGTGCTAT 
GTGGCTCTGG ACTTCGAGAA CGAGATGGCA ACAGCTGCCT CTTCTTCCTC TCTGGAGAAG 
AGCTATGAAT TGCCTGATGG GCAGGTCATC ACCATCGGCA ATGAACGTTT CCGTTGCCCA 
GAAACCCTTT TCCAGCCTTC CTTCATTGGT ATGGAATCCG CTGGTATTCA TGAGACAACC 
TACAACAGCA TCATGAAGTG CGATATTGAC ATCAGGAAGG ACCTGTATGC CAACAATGTC 
ATGTCTGGTG GCACCACCAT GTACCCCGGT ATTGCTGACC GTATGCAGAA GGAAATCACT 
GCTCTGGCTC CCAGCACAAT GAAGATCAAG ATCATTGCCC CCCCAGAGCG TAAGTACTCT 
GTCTGGATCG GAGGGTCCAT TTTGGCTTCC CTGTCCACCT TCCAACAGAT GTGGATCACA 
AAACAGGAAT ACGATGAAGC CGGCCCATCC ATTGTCCACC GTAAATGCTT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013915303.1
CDS	453..1586
Translation	MCDEDETTALVCDNGSGLVKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPTLLTEAPLNPKANREKMTQIMFETFNVPAMYVAIQAVLSLYASGRTTGIVLDSGDGVTHNVPIYEGYALPHAIMRLDLAGRDLTDYLMKILTERGYSFVTTAEREIVRDIKEKLCYVALDFENEMATAASSSSLEKSYELPDGQVITIGNERFRCPETLFQPSFIGMESAGIHETTYNSIMKCDIDIRKDLYANNVMSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWITKQEYDEAGPSIVHRKCF

Target ORF information:

RefSeq Version	XM_014059828.1
Organism	Thamnophis sirtalis
Definition	Thamnophis sirtalis actin, alpha 1, skeletal muscle (ACTA1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014059828.1

ORF Insert Sequence:

ATGTGTGATG AGGATGAGAC CACCGCACTT GTGTGCGACA ACGGCTCTGG CTTGGTGAAA 
GCTGGCTTTG CCGGGGATGA CGCCCCTAGG GCTGTCTTTC CCTCCATTGT TGGCCGCCCA 
CGTCATCAGG GTGTCATGGT GGGTATGGGT CAAAAAGACT CCTACGTAGG GGATGAAGCC 
CAGAGCAAAA GAGGTATCCT GACCCTGAAG TACCCCATTG AACATGGCAT CATCACCAAC 
TGGGATGACA TGGAGAAGAT TTGGCATCAC ACTTTCTACA ATGAGCTCCG TGTTGCCCCA 
GAGGAGCACC CCACCCTGCT CACTGAAGCC CCTCTTAACC CCAAGGCCAA TCGTGAAAAG 
ATGACCCAGA TCATGTTTGA GACCTTCAAC GTCCCTGCCA TGTATGTGGC CATCCAGGCT 
GTGCTGTCCC TCTATGCCTC TGGCCGTACC ACCGGTATTG TGCTGGACTC TGGCGATGGT 
GTCACCCACA ATGTACCCAT CTATGAGGGT TATGCCCTGC CCCATGCCAT CATGCGTTTG 
GACTTGGCTG GTCGGGATCT CACTGACTAC CTGATGAAGA TCCTGACTGA GAGAGGCTAC 
TCTTTTGTCA CCACTGCTGA ACGTGAGATT GTCCGTGACA TCAAGGAAAA GCTGTGCTAT 
GTGGCTCTGG ACTTCGAGAA CGAGATGGCA ACAGCTGCCT CTTCTTCCTC TCTGGAGAAG 
AGCTATGAAT TGCCTGATGG GCAGGTCATC ACCATCGGCA ATGAACGTTT CCGTTGCCCA 
GAAACCCTTT TCCAGCCTTC CTTCATTGGT ATGGAATCCG CTGGTATTCA TGAGACAACC 
TACAACAGCA TCATGAAGTG CGATATTGAC ATCAGGAAGG ACCTGTATGC CAACAATGTC 
ATGTCTGGTG GCACCACCAT GTACCCCGGT ATTGCTGACC GTATGCAGAA GGAAATCACT 
GCTCTGGCTC CCAGCACAAT GAAGATCAAG ATCATTGCCC CCCCAGAGCG TAAGTACTCT 
GTCTGGATCG GAGGGTCCAT TTTGGCTTCC CTGTCCACCT TCCAACAGAT GTGGATCACA 
AAACAGGAAT ACGATGAAGC CGGCCCATCC ATTGTCCACC GTAAATGCTT CTAA