FMNL1 cDNA ORF clone, Thamnophis sirtalis

Gene
Clones
gRNAs

Summary

Gene Symbol	FMNL1
Entrez Gene ID	106542521
Full Name	formin like 1
Gene Type	protein-coding
Organism	Thamnophis sirtalis

mRNA and Protein(s)

mRNA	Protein	Name
XM_014058296.1	XP_013913771.1	formin-like protein 1 isoform X1
XM_014058299.1	XP_013913774.1	formin-like protein 1 isoform X4
XM_014058300.1	XP_013913775.1	formin-like protein 1 isoform X5
XM_014058301.1	XP_013913776.1	formin-like protein 1 isoform X6
XM_014058303.1	XP_013913778.1	formin-like protein 1 isoform X8
XM_014058297.1	XP_013913772.1	formin-like protein 1 isoform X2
XM_014058298.1	XP_013913773.1	formin-like protein 1 isoform X3
XM_014058302.1	XP_013913777.1	formin-like protein 1 isoform X7

Pathways from BioSystems
Gene Ontology

The following FMNL1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the FMNL1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OTh18456	XM_014058302.1 Latest version!	Thamnophis sirtalis formin-like 1 (FMNL1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OTh18450	XM_014058296.1 Latest version!	Thamnophis sirtalis formin-like 1 (FMNL1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OTh18451	XM_014058297.1 Latest version!	Thamnophis sirtalis formin-like 1 (FMNL1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OTh18452	XM_014058298.1 Latest version!	Thamnophis sirtalis formin-like 1 (FMNL1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OTh18453	XM_014058299.1 Latest version!	Thamnophis sirtalis formin-like 1 (FMNL1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OTh18454	XM_014058300.1 Latest version!	Thamnophis sirtalis formin-like 1 (FMNL1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OTh18455	XM_014058301.1 Latest version!	Thamnophis sirtalis formin-like 1 (FMNL1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OTh18457	XM_014058303.1 Latest version!	Thamnophis sirtalis formin-like 1 (FMNL1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

FMNL1 ( XM_014058302.1 ) cDNA ORF clone, Thamnophis sirtalis -> XP_013913777.1 Thamnophis sirtalis formin-like 1 (FMNL1), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OTh18456
Clone ID Related Accession (Same CDS sequence)	XM_014058302.1
Accession Version	XM_014058302.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2694bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-10
Organism	Thamnophis sirtalis
Product	formin-like protein 1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_013658128.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Thamnophis sirtalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTCTCC GTGCCATTAT GAATTATCAG TCTGGATTCA GCCTTGTGAT GAACCACCCT 
GCCTGTGTAA ATGAGATCAC ACTCAGCCTC AACAATAAGA ATCCCAGAAC TAAAGCTCTT 
GTCCTTGAGC TATTGGCTGC TGTTTGCTTG GTCCGAGGAG GACACGACAT TATTCTGGCT 
GCCTTTGACA ACTTCAAAGA GGTTTGTGGT GAGAAGAACC GTTTTGAGAA GCTGATGGAG 
TATTTCCGAA ATGAAGACAG CAACATAGAT TTCATGGTGG CTTGTATGCA GTTCATCAAT 
ATTGTGGTTC ATTCTGTGGA GAACATGAAC TTCCGTGCCT TCTTACAATA TGAGTTTACA 
CAGTTGGGAC TTGATGAGTA CCTACAGAAG CTGCGTTGTA CAGAAAGTGA TAAACTGCAA 
GTGCAGATCC AAGCCTATCT GGATAATATC TTTGATGTTG GTGCCTTACT GGAGGATACC 
GAGACCAAAA ATGCCATCCT GGAACACATA GAGGATTTGC AAGAAGAGGT TGGACAGCTC 
ACTGAGAAAC TCCAGGATGC AGAAAACGAA TCCGAAGCCA AAATTGCTGA GCTAGAGAAA 
CAGCTGTATC AGTCACGCAA TGAGCTGGAG ATCCTGAGAG CAAGAACTAG TGATGAGCCG 
GAGAAGCTAG ATGAAATCAG CAAAGTTAAC AAGCCAGAAA GTCCTTCTTG GTTCCCAATG 
CAGCTTCCAC CAGAGTCAAA AATAACTCCA CAATACTCTG CCTTGGAGCA CAATCTGGAA 
GAACTAGAAG ACAGAGGTTT TATCCGGATC CAGCGTGGGC CTGGCAGTGA TAGCATCATT 
GGCATTGAGG TCCTCCCTGT TATTAATATT GAAGCATCTT CGTCAACTAG CACAGATTAT 
CCCTCCCCAT CTACATCTCC TGTCCGCAGT CTTGCTCCAG AAATACCACC AGCACCACCT 
CTACCTTCCA CGAAAGGCAG TGATTCTGGG GCTTTCTATT CTTCTCAAGA GGAGGGGTTG 
CCACCTATTC CAACTTCTCT TCCACCACCA CCACCACCAC CCTTACCAGG GATCAATGAG 
CCTCCACCTG CCCCACCACT GCCTGTGATT GTAACTACAT CTCAAGCTTC ATCAACGCCT 
CCTCCTCCTC CGCCACCACC CCTCTTAACA TTTGGTGGCC CAGTTGCTCC TCCTCCTCCA 
CCACCGCCTC CCATGATTGA TGGAGCAACT TTGCCAGCCA GTACAGTTAC TGCAGCTGTC 
AAGATCAAGA AGCCTATACA GACCAAATTC CGTATGCCTG TCTTCAATTG GGTTGCTTTG 
AAGCCTAACC AGATCAATGG GACCATCTTC AATGAACTCA ATGATGAAAA AGTCCTTCAG 
GAGCTGGACA TGAATGACTT TGAAGAACAA TTCAAAACCA AAGCTCAGGG TCCAGGCAAA 
GAATTCAGTT CCATAAAGAC CAAGACTTTA CAGAAAGCTC CCACTAAGGT TTCACTGATT 
GAATGCAACC GGGCCAAGAA CCTGGCCATC ACCCTCCGTA AAGGGGGGCT CAGTATCGAC 
AGCATTTGCA AAGCCATTCA GACATACGAC TTACAAACCC TCAATCTGGA TTTTCTGGAG 
TTACTGATGC GCTTCATCCC AACGGAATAT GAGATGACAC TAATCCGCAA ATATGAAAAA 
GAGCAACGTC CACTGGATGA GCTTTCAGAT GAGGACCAAT TCATGATCAA ATTCAGCAAA 
ATTCCCCGCC TTACAGAACG CATGAACATC ATGACATTCT TAGGCAGTTT TGCAGATACC 
GTACAACTCC TTCTGCCACA ACTAAATTTT GTGATTGTTG CTTCAATGTC AGTCAAGTCA 
TCTACCAAAC TGCGTCAGAT TTTAGAGATT GTCCTGGCAT TTGGGAATTA TTTGAACAGC 
AGCAAACGGG GAGCGGCTTA TGGATTCCGG TTGCAGAGCC TTGATGCGCT TCTGGATATG 
AAATCAACAG ACCGGAAACA GACACTACTT CATTATATAG TGCGGGTGAT CCAAGAAAAA 
TACCCTGAGT TCCTGAACTT TTCCAGTGAG CTGCATTTTC TGGACAAAGC AGGGGCAGTG 
TCACTGGACA GTGTGCTTCA AGATGTGAGA AGCCTACAGC GGGGCATGGA GCTAACACGC 
AAAGAATTCA TGCGCCAGGA TGACAGTATC ATACTCAAGG ATTTCCTAAA GACAAACATA 
GAAATGATGG AAAAGCTTCA GGCAGACAGT AAAACTGCAC AGGAGGCATA CGAGTCAACA 
GTGGAATACT TTGGGGAGAA TCCCAAGACT ACTCCTCCAA CTATGTTCTT CCCTACATTT 
GTACGCTTTA TTAAAGCATA CCAGAAAGCA GAGGAAGATA TAAAAGTGTG GAAGAAGCAA 
GAAGCAGCTG CCAAAGAAGA GGAGCAAGTT TCCCCCGGCA AAGGGGAACA GGCAGTACCC 
AAGCAATCTC CCACCCATAA GGCCAAGCGA CAACAGATGG ACCTCATAAC TGAGCTGAAG 
AAAAAGCAGA TGGTGAAAGA GCCTCTCATT TATGAAGAAA AAGATGGTGC CATTGAAGAC 
ATTATTTCAG GTCTACGTAT CCAGCCATAC CGGCGGGGGG ACACTGGTCG GCACAGTGGC 
AAAAAACGAA CCACTGGGCA GATTCTCCAG GCAACCTCCG AGATCTCACT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013913777.1
CDS	402..3095
Translation	MCLRAIMNYQSGFSLVMNHPACVNEITLSLNNKNPRTKALVLELLAAVCLVRGGHDIILAAFDNFKEVCGEKNRFEKLMEYFRNEDSNIDFMVACMQFINIVVHSVENMNFRAFLQYEFTQLGLDEYLQKLRCTESDKLQVQIQAYLDNIFDVGALLEDTETKNAILEHIEDLQEEVGQLTEKLQDAENESEAKIAELEKQLYQSRNELEILRARTSDEPEKLDEISKVNKPESPSWFPMQLPPESKITPQYSALEHNLEELEDRGFIRIQRGPGSDSIIGIEVLPVINIEASSSTSTDYPSPSTSPVRSLAPEIPPAPPLPSTKGSDSGAFYSSQEEGLPPIPTSLPPPPPPPLPGINEPPPAPPLPVIVTTSQASSTPPPPPPPPLLTFGGPVAPPPPPPPPMIDGATLPASTVTAAVKIKKPIQTKFRMPVFNWVALKPNQINGTIFNELNDEKVLQELDMNDFEEQFKTKAQGPGKEFSSIKTKTLQKAPTKVSLIECNRAKNLAITLRKGGLSIDSICKAIQTYDLQTLNLDFLELLMRFIPTEYEMTLIRKYEKEQRPLDELSDEDQFMIKFSKIPRLTERMNIMTFLGSFADTVQLLLPQLNFVIVASMSVKSSTKLRQILEIVLAFGNYLNSSKRGAAYGFRLQSLDALLDMKSTDRKQTLLHYIVRVIQEKYPEFLNFSSELHFLDKAGAVSLDSVLQDVRSLQRGMELTRKEFMRQDDSIILKDFLKTNIEMMEKLQADSKTAQEAYESTVEYFGENPKTTPPTMFFPTFVRFIKAYQKAEEDIKVWKKQEAAAKEEEQVSPGKGEQAVPKQSPTHKAKRQQMDLITELKKKQMVKEPLIYEEKDGAIEDIISGLRIQPYRRGDTGRHSGKKRTTGQILQATSEISL

Target ORF information:

RefSeq Version	XM_014058302.1
Organism	Thamnophis sirtalis
Definition	Thamnophis sirtalis formin-like 1 (FMNL1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014058302.1

ORF Insert Sequence:

ATGTGTCTCC GTGCCATTAT GAATTATCAG TCTGGATTCA GCCTTGTGAT GAACCACCCT 
GCCTGTGTAA ATGAGATCAC ACTCAGCCTC AACAATAAGA ATCCCAGAAC TAAAGCTCTT 
GTCCTTGAGC TATTGGCTGC TGTTTGCTTG GTCCGAGGAG GACACGACAT TATTCTGGCT 
GCCTTTGACA ACTTCAAAGA GGTTTGTGGT GAGAAGAACC GTTTTGAGAA GCTGATGGAG 
TATTTCCGAA ATGAAGACAG CAACATAGAT TTCATGGTGG CTTGTATGCA GTTCATCAAT 
ATTGTGGTTC ATTCTGTGGA GAACATGAAC TTCCGTGCCT TCTTACAATA TGAGTTTACA 
CAGTTGGGAC TTGATGAGTA CCTACAGAAG CTGCGTTGTA CAGAAAGTGA TAAACTGCAA 
GTGCAGATCC AAGCCTATCT GGATAATATC TTTGATGTTG GTGCCTTACT GGAGGATACC 
GAGACCAAAA ATGCCATCCT GGAACACATA GAGGATTTGC AAGAAGAGGT TGGACAGCTC 
ACTGAGAAAC TCCAGGATGC AGAAAACGAA TCCGAAGCCA AAATTGCTGA GCTAGAGAAA 
CAGCTGTATC AGTCACGCAA TGAGCTGGAG ATCCTGAGAG CAAGAACTAG TGATGAGCCG 
GAGAAGCTAG ATGAAATCAG CAAAGTTAAC AAGCCAGAAA GTCCTTCTTG GTTCCCAATG 
CAGCTTCCAC CAGAGTCAAA AATAACTCCA CAATACTCTG CCTTGGAGCA CAATCTGGAA 
GAACTAGAAG ACAGAGGTTT TATCCGGATC CAGCGTGGGC CTGGCAGTGA TAGCATCATT 
GGCATTGAGG TCCTCCCTGT TATTAATATT GAAGCATCTT CGTCAACTAG CACAGATTAT 
CCCTCCCCAT CTACATCTCC TGTCCGCAGT CTTGCTCCAG AAATACCACC AGCACCACCT 
CTACCTTCCA CGAAAGGCAG TGATTCTGGG GCTTTCTATT CTTCTCAAGA GGAGGGGTTG 
CCACCTATTC CAACTTCTCT TCCACCACCA CCACCACCAC CCTTACCAGG GATCAATGAG 
CCTCCACCTG CCCCACCACT GCCTGTGATT GTAACTACAT CTCAAGCTTC ATCAACGCCT 
CCTCCTCCTC CGCCACCACC CCTCTTAACA TTTGGTGGCC CAGTTGCTCC TCCTCCTCCA 
CCACCGCCTC CCATGATTGA TGGAGCAACT TTGCCAGCCA GTACAGTTAC TGCAGCTGTC 
AAGATCAAGA AGCCTATACA GACCAAATTC CGTATGCCTG TCTTCAATTG GGTTGCTTTG 
AAGCCTAACC AGATCAATGG GACCATCTTC AATGAACTCA ATGATGAAAA AGTCCTTCAG 
GAGCTGGACA TGAATGACTT TGAAGAACAA TTCAAAACCA AAGCTCAGGG TCCAGGCAAA 
GAATTCAGTT CCATAAAGAC CAAGACTTTA CAGAAAGCTC CCACTAAGGT TTCACTGATT 
GAATGCAACC GGGCCAAGAA CCTGGCCATC ACCCTCCGTA AAGGGGGGCT CAGTATCGAC 
AGCATTTGCA AAGCCATTCA GACATACGAC TTACAAACCC TCAATCTGGA TTTTCTGGAG 
TTACTGATGC GCTTCATCCC AACGGAATAT GAGATGACAC TAATCCGCAA ATATGAAAAA 
GAGCAACGTC CACTGGATGA GCTTTCAGAT GAGGACCAAT TCATGATCAA ATTCAGCAAA 
ATTCCCCGCC TTACAGAACG CATGAACATC ATGACATTCT TAGGCAGTTT TGCAGATACC 
GTACAACTCC TTCTGCCACA ACTAAATTTT GTGATTGTTG CTTCAATGTC AGTCAAGTCA 
TCTACCAAAC TGCGTCAGAT TTTAGAGATT GTCCTGGCAT TTGGGAATTA TTTGAACAGC 
AGCAAACGGG GAGCGGCTTA TGGATTCCGG TTGCAGAGCC TTGATGCGCT TCTGGATATG 
AAATCAACAG ACCGGAAACA GACACTACTT CATTATATAG TGCGGGTGAT CCAAGAAAAA 
TACCCTGAGT TCCTGAACTT TTCCAGTGAG CTGCATTTTC TGGACAAAGC AGGGGCAGTG 
TCACTGGACA GTGTGCTTCA AGATGTGAGA AGCCTACAGC GGGGCATGGA GCTAACACGC 
AAAGAATTCA TGCGCCAGGA TGACAGTATC ATACTCAAGG ATTTCCTAAA GACAAACATA 
GAAATGATGG AAAAGCTTCA GGCAGACAGT AAAACTGCAC AGGAGGCATA CGAGTCAACA 
GTGGAATACT TTGGGGAGAA TCCCAAGACT ACTCCTCCAA CTATGTTCTT CCCTACATTT 
GTACGCTTTA TTAAAGCATA CCAGAAAGCA GAGGAAGATA TAAAAGTGTG GAAGAAGCAA 
GAAGCAGCTG CCAAAGAAGA GGAGCAAGTT TCCCCCGGCA AAGGGGAACA GGCAGTACCC 
AAGCAATCTC CCACCCATAA GGCCAAGCGA CAACAGATGG ACCTCATAAC TGAGCTGAAG 
AAAAAGCAGA TGGTGAAAGA GCCTCTCATT TATGAAGAAA AAGATGGTGC CATTGAAGAC 
ATTATTTCAG GTCTACGTAT CCAGCCATAC CGGCGGGGGG ACACTGGTCG GCACAGTGGC 
AAAAAACGAA CCACTGGGCA GATTCTCCAG GCAACCTCCG AGATCTCACT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FMNL1 ( XM_014058296.1 ) cDNA ORF clone, Thamnophis sirtalis -> XP_013913771.1 Thamnophis sirtalis formin-like 1 (FMNL1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OTh18450
Clone ID Related Accession (Same CDS sequence)	XM_014058296.1
Accession Version	XM_014058296.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3522bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-10
Organism	Thamnophis sirtalis
Product	formin-like protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_013658128.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Thamnophis sirtalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTAATG CAGCCAGCAG CCTGGAAATG TCTCCAGCGA AGGAGATTAA AACCCAACCG 
ACTGCTTCTT CGCCTTTACC CCCTAAACAG CCTGCACCCC CCAAACTACC GATGCCTAAT 
GCTGAGGAAT TGGAAGAACG ATTCGGACAT GTTCTGAATT CAATGAATCT GCCACCAGAC 
AAAATGAAGC TTCTGAGCCA GTATGATAAT GAAAAGAAAT GGGAATTAAT ATGTGACCAG 
GAACGATTCC AGGTCAAAAA CCCACCATCT GCCTATATCC AGAAACTGAA AAGCTATCTA 
GACACAGGTG GATTCAGTGG AAAGTTTAAG AGACGAGTTC AAGAATCTAC CCAGATTCTT 
CGGGAGTTAG AGATATCCCT GAGAACAAAC CACATTGGTT GGGTCCAGGA GTTCCTAAAT 
GAAGAGAATA GAGGTCTTGA TGTGTTGGTG GATTATCTTG CTTTTGCCCA GTGCTCTGTC 
ACGTATGATA TGGACAGCAC TGACAATGGG AGCCCTGGGT CGGACAAGGG AAAGGCCCTG 
GACAGATCTG TAGAGGACCT CAGCAGAAGC AATTGCTCTC CCACACAGGG TTCCATCAAA 
GCGAGGCACT TGACTGTCAG GTGTTGCCAT TTGAACCAGC AGATCCAAAC ATTCTTTCAA 
CAAGTCCACC CCAGCATCCC CTCCTCCAGC GCCCCTGGCA CCGAGCAGGA CTGTAAGCCC 
AGGCCACCTG TCAACTCTTA CTCACCCCGA TTCAATCCGG CACACAGCAG GAAGGCATTA 
CGGAACTCAC GCATCGTCAG CCAGAAGGAT GATGTTCATG TGTGCATCAT GTGTCTCCGT 
GCCATTATGA ATTATCAGTC TGGATTCAGC CTTGTGATGA ACCACCCTGC CTGTGTAAAT 
GAGATCACAC TCAGCCTCAA CAATAAGAAT CCCAGAACTA AAGCTCTTGT CCTTGAGCTA 
TTGGCTGCTG TTTGCTTGGT CCGAGGAGGA CACGACATTA TTCTGGCTGC CTTTGACAAC 
TTCAAAGAGG TTTGTGGTGA GAAGAACCGT TTTGAGAAGC TGATGGAGTA TTTCCGAAAT 
GAAGACAGCA ACATAGATTT CATGGTGGCT TGTATGCAGT TCATCAATAT TGTGGTTCAT 
TCTGTGGAGA ACATGAACTT CCGTGCCTTC TTACAATATG AGTTTACACA GTTGGGACTT 
GATGAGTACC TACAGAAGCT GCGTTGTACA GAAAGTGATA AACTGCAAGT GCAGATCCAA 
GCCTATCTGG ATAATATCTT TGATGTTGGT GCCTTACTGG AGGATACCGA GACCAAAAAT 
GCCATCCTGG AACACATAGA GGATTTGCAA GAAGAGGTTG GACAGCTCAC TGAGAAACTC 
CAGGATGCAG AAAACGAATC CGAAGCCAAA ATTGCTGAGC TAGAGAAACA GCTGTATCAG 
TCACGCAATG AGCTGGAGAT CCTGAGAGCA AGAACTAGTG ATGAGCCGGA GAAGCTAGAT 
GAAATCAGCA AAGTTAACAA GCCAGAAAGT CCTTCTTGGT TCCCAATGCA GCTTCCACCA 
GAGTCAAAAA TAACTCCACA ATACTCTGCC TTGGAGCACA ATCTGGAAGA ACTAGAAGAC 
AGAGGTTTTA TCCGGATCCA GCGTGGGCCT GGCAGTGATA GCATCATTGG CATTGAGGTC 
CTCCCTGTTA TTAATATTGA AGCATCTTCG TCAACTAGCA CAGATTATCC CTCCCCATCT 
ACATCTCCTG TCCGCAGTCT TGCTCCAGAA ATACCACCAG CACCACCTCT ACCTTCCACG 
AAAGGCAGTG ATTCTGGGGC TTTCTATTCT TCTCAAGAGG AGGGGTTGCC ACCTATTCCA 
ACTTCTCTTC CACCACCACC ACCACCACCC TTACCAGGGA TCAATGAGCC TCCACCTGCC 
CCACCACTGC CTGTGATTGT AACTACATCT CAAGCTTCAT CAACGCCTCC TCCTCCTCCG 
CCACCACCCC TCTTAACATT TGGTGGCCCA GTTGCTCCTC CTCCTCCACC ACCGCCTCCC 
ATGATTGATG GAGCAACTTT GCCAGCCAGT ACAGTTACTG CAGCTGTCAA GATCAAGAAG 
CCTATACAGA CCAAATTCCG TATGCCTGTC TTCAATTGGG TTGCTTTGAA GCCTAACCAG 
ATCAATGGGA CCATCTTCAA TGAACTCAAT GATGAAAAAG TCCTTCAGGA GCTGGACATG 
AATGACTTTG AAGAACAATT CAAAACCAAA GCTCAGGGTC CAGGCAAAGA ATTCAGTTCC 
ATAAAGACCA AGACTTTACA GAAAGCTCCC ACTAAGGTTT CACTGATTGA ATGCAACCGG 
GCCAAGAACC TGGCCATCAC CCTCCGTAAA GGGGGGCTCA GTATCGACAG CATTTGCAAA 
GCCATTCAGA CATACGACTT ACAAACCCTC AATCTGGATT TTCTGGAGTT ACTGATGCGC 
TTCATCCCAA CGGAATATGA GATGACACTA ATCCGCAAAT ATGAAAAAGA GCAACGTCCA 
CTGGATGAGC TTTCAGATGA GGACCAATTC ATGATCAAAT TCAGCAAAAT TCCCCGCCTT 
ACAGAACGCA TGAACATCAT GACATTCTTA GGCAGTTTTG CAGATACCGT ACAACTCCTT 
CTGCCACAAC TAAATTTTGT GATTGTTGCT TCAATGTCAG TCAAGTCATC TACCAAACTG 
CGTCAGATTT TAGAGATTGT CCTGGCATTT GGGAATTATT TGAACAGCAG CAAACGGGGA 
GCGGCTTATG GATTCCGGTT GCAGAGCCTT GATGCGCTTC TGGATATGAA ATCAACAGAC 
CGGAAACAGA CACTACTTCA TTATATAGTG CGGGTGATCC AAGAAAAATA CCCTGAGTTC 
CTGAACTTTT CCAGTGAGCT GCATTTTCTG GACAAAGCAG GGGCAGTGTC ACTGGACAGT 
GTGCTTCAAG ATGTGAGAAG CCTACAGCGG GGCATGGAGC TAACACGCAA AGAATTCATG 
CGCCAGGATG ACAGTATCAT ACTCAAGGAT TTCCTAAAGA CAAACATAGA AATGATGGAA 
AAGCTTCAGG CAGACAGTAA AACTGCACAG GAGGCATACG AGTCAACAGT GGAATACTTT 
GGGGAGAATC CCAAGACTAC TCCTCCAACT ATGTTCTTCC CTACATTTGT ACGCTTTATT 
AAAGCATACC AGAAAGCAGA GGAAGATATA AAAGTGTGGA AGAAGCAAGA AGCAGCTGCC 
AAAGAAGAGG AGCAAGTTTC CCCCGGCAAA GGGGAACAGG CAGTACCCAA GCAATCTCCC 
ACCCATAAGG CCAAGCGACA ACAGATGGAC CTCATAACTG AGCTGAAGAA AAAGCAGATG 
GTGAAAGAGC CTCTCATTTA TGAAGAAAAA GATGGTGCCA TTGAAGACAT TATTTCAGGT 
CTACGTATCC AGCCATACCG GCGGGGGGAC ACTGGTCGGC ACAGTGGCAA AAAACGAACC 
ACTGGGCAGA TTCTCCAGGC AACCTCCGAG ATCTCACTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013913771.1
CDS	811..4332
Translation	MGNAASSLEMSPAKEIKTQPTASSPLPPKQPAPPKLPMPNAEELEERFGHVLNSMNLPPDKMKLLSQYDNEKKWELICDQERFQVKNPPSAYIQKLKSYLDTGGFSGKFKRRVQESTQILRELEISLRTNHIGWVQEFLNEENRGLDVLVDYLAFAQCSVTYDMDSTDNGSPGSDKGKALDRSVEDLSRSNCSPTQGSIKARHLTVRCCHLNQQIQTFFQQVHPSIPSSSAPGTEQDCKPRPPVNSYSPRFNPAHSRKALRNSRIVSQKDDVHVCIMCLRAIMNYQSGFSLVMNHPACVNEITLSLNNKNPRTKALVLELLAAVCLVRGGHDIILAAFDNFKEVCGEKNRFEKLMEYFRNEDSNIDFMVACMQFINIVVHSVENMNFRAFLQYEFTQLGLDEYLQKLRCTESDKLQVQIQAYLDNIFDVGALLEDTETKNAILEHIEDLQEEVGQLTEKLQDAENESEAKIAELEKQLYQSRNELEILRARTSDEPEKLDEISKVNKPESPSWFPMQLPPESKITPQYSALEHNLEELEDRGFIRIQRGPGSDSIIGIEVLPVINIEASSSTSTDYPSPSTSPVRSLAPEIPPAPPLPSTKGSDSGAFYSSQEEGLPPIPTSLPPPPPPPLPGINEPPPAPPLPVIVTTSQASSTPPPPPPPPLLTFGGPVAPPPPPPPPMIDGATLPASTVTAAVKIKKPIQTKFRMPVFNWVALKPNQINGTIFNELNDEKVLQELDMNDFEEQFKTKAQGPGKEFSSIKTKTLQKAPTKVSLIECNRAKNLAITLRKGGLSIDSICKAIQTYDLQTLNLDFLELLMRFIPTEYEMTLIRKYEKEQRPLDELSDEDQFMIKFSKIPRLTERMNIMTFLGSFADTVQLLLPQLNFVIVASMSVKSSTKLRQILEIVLAFGNYLNSSKRGAAYGFRLQSLDALLDMKSTDRKQTLLHYIVRVIQEKYPEFLNFSSELHFLDKAGAVSLDSVLQDVRSLQRGMELTRKEFMRQDDSIILKDFLKTNIEMMEKLQADSKTAQEAYESTVEYFGENPKTTPPTMFFPTFVRFIKAYQKAEEDIKVWKKQEAAAKEEEQVSPGKGEQAVPKQSPTHKAKRQQMDLITELKKKQMVKEPLIYEEKDGAIEDIISGLRIQPYRRGDTGRHSGKKRTTGQILQATSEISL

Target ORF information:

RefSeq Version	XM_014058296.1
Organism	Thamnophis sirtalis
Definition	Thamnophis sirtalis formin-like 1 (FMNL1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014058296.1

ORF Insert Sequence:

ATGGGTAATG CAGCCAGCAG CCTGGAAATG TCTCCAGCGA AGGAGATTAA AACCCAACCG 
ACTGCTTCTT CGCCTTTACC CCCTAAACAG CCTGCACCCC CCAAACTACC GATGCCTAAT 
GCTGAGGAAT TGGAAGAACG ATTCGGACAT GTTCTGAATT CAATGAATCT GCCACCAGAC 
AAAATGAAGC TTCTGAGCCA GTATGATAAT GAAAAGAAAT GGGAATTAAT ATGTGACCAG 
GAACGATTCC AGGTCAAAAA CCCACCATCT GCCTATATCC AGAAACTGAA AAGCTATCTA 
GACACAGGTG GATTCAGTGG AAAGTTTAAG AGACGAGTTC AAGAATCTAC CCAGATTCTT 
CGGGAGTTAG AGATATCCCT GAGAACAAAC CACATTGGTT GGGTCCAGGA GTTCCTAAAT 
GAAGAGAATA GAGGTCTTGA TGTGTTGGTG GATTATCTTG CTTTTGCCCA GTGCTCTGTC 
ACGTATGATA TGGACAGCAC TGACAATGGG AGCCCTGGGT CGGACAAGGG AAAGGCCCTG 
GACAGATCTG TAGAGGACCT CAGCAGAAGC AATTGCTCTC CCACACAGGG TTCCATCAAA 
GCGAGGCACT TGACTGTCAG GTGTTGCCAT TTGAACCAGC AGATCCAAAC ATTCTTTCAA 
CAAGTCCACC CCAGCATCCC CTCCTCCAGC GCCCCTGGCA CCGAGCAGGA CTGTAAGCCC 
AGGCCACCTG TCAACTCTTA CTCACCCCGA TTCAATCCGG CACACAGCAG GAAGGCATTA 
CGGAACTCAC GCATCGTCAG CCAGAAGGAT GATGTTCATG TGTGCATCAT GTGTCTCCGT 
GCCATTATGA ATTATCAGTC TGGATTCAGC CTTGTGATGA ACCACCCTGC CTGTGTAAAT 
GAGATCACAC TCAGCCTCAA CAATAAGAAT CCCAGAACTA AAGCTCTTGT CCTTGAGCTA 
TTGGCTGCTG TTTGCTTGGT CCGAGGAGGA CACGACATTA TTCTGGCTGC CTTTGACAAC 
TTCAAAGAGG TTTGTGGTGA GAAGAACCGT TTTGAGAAGC TGATGGAGTA TTTCCGAAAT 
GAAGACAGCA ACATAGATTT CATGGTGGCT TGTATGCAGT TCATCAATAT TGTGGTTCAT 
TCTGTGGAGA ACATGAACTT CCGTGCCTTC TTACAATATG AGTTTACACA GTTGGGACTT 
GATGAGTACC TACAGAAGCT GCGTTGTACA GAAAGTGATA AACTGCAAGT GCAGATCCAA 
GCCTATCTGG ATAATATCTT TGATGTTGGT GCCTTACTGG AGGATACCGA GACCAAAAAT 
GCCATCCTGG AACACATAGA GGATTTGCAA GAAGAGGTTG GACAGCTCAC TGAGAAACTC 
CAGGATGCAG AAAACGAATC CGAAGCCAAA ATTGCTGAGC TAGAGAAACA GCTGTATCAG 
TCACGCAATG AGCTGGAGAT CCTGAGAGCA AGAACTAGTG ATGAGCCGGA GAAGCTAGAT 
GAAATCAGCA AAGTTAACAA GCCAGAAAGT CCTTCTTGGT TCCCAATGCA GCTTCCACCA 
GAGTCAAAAA TAACTCCACA ATACTCTGCC TTGGAGCACA ATCTGGAAGA ACTAGAAGAC 
AGAGGTTTTA TCCGGATCCA GCGTGGGCCT GGCAGTGATA GCATCATTGG CATTGAGGTC 
CTCCCTGTTA TTAATATTGA AGCATCTTCG TCAACTAGCA CAGATTATCC CTCCCCATCT 
ACATCTCCTG TCCGCAGTCT TGCTCCAGAA ATACCACCAG CACCACCTCT ACCTTCCACG 
AAAGGCAGTG ATTCTGGGGC TTTCTATTCT TCTCAAGAGG AGGGGTTGCC ACCTATTCCA 
ACTTCTCTTC CACCACCACC ACCACCACCC TTACCAGGGA TCAATGAGCC TCCACCTGCC 
CCACCACTGC CTGTGATTGT AACTACATCT CAAGCTTCAT CAACGCCTCC TCCTCCTCCG 
CCACCACCCC TCTTAACATT TGGTGGCCCA GTTGCTCCTC CTCCTCCACC ACCGCCTCCC 
ATGATTGATG GAGCAACTTT GCCAGCCAGT ACAGTTACTG CAGCTGTCAA GATCAAGAAG 
CCTATACAGA CCAAATTCCG TATGCCTGTC TTCAATTGGG TTGCTTTGAA GCCTAACCAG 
ATCAATGGGA CCATCTTCAA TGAACTCAAT GATGAAAAAG TCCTTCAGGA GCTGGACATG 
AATGACTTTG AAGAACAATT CAAAACCAAA GCTCAGGGTC CAGGCAAAGA ATTCAGTTCC 
ATAAAGACCA AGACTTTACA GAAAGCTCCC ACTAAGGTTT CACTGATTGA ATGCAACCGG 
GCCAAGAACC TGGCCATCAC CCTCCGTAAA GGGGGGCTCA GTATCGACAG CATTTGCAAA 
GCCATTCAGA CATACGACTT ACAAACCCTC AATCTGGATT TTCTGGAGTT ACTGATGCGC 
TTCATCCCAA CGGAATATGA GATGACACTA ATCCGCAAAT ATGAAAAAGA GCAACGTCCA 
CTGGATGAGC TTTCAGATGA GGACCAATTC ATGATCAAAT TCAGCAAAAT TCCCCGCCTT 
ACAGAACGCA TGAACATCAT GACATTCTTA GGCAGTTTTG CAGATACCGT ACAACTCCTT 
CTGCCACAAC TAAATTTTGT GATTGTTGCT TCAATGTCAG TCAAGTCATC TACCAAACTG 
CGTCAGATTT TAGAGATTGT CCTGGCATTT GGGAATTATT TGAACAGCAG CAAACGGGGA 
GCGGCTTATG GATTCCGGTT GCAGAGCCTT GATGCGCTTC TGGATATGAA ATCAACAGAC 
CGGAAACAGA CACTACTTCA TTATATAGTG CGGGTGATCC AAGAAAAATA CCCTGAGTTC 
CTGAACTTTT CCAGTGAGCT GCATTTTCTG GACAAAGCAG GGGCAGTGTC ACTGGACAGT 
GTGCTTCAAG ATGTGAGAAG CCTACAGCGG GGCATGGAGC TAACACGCAA AGAATTCATG 
CGCCAGGATG ACAGTATCAT ACTCAAGGAT TTCCTAAAGA CAAACATAGA AATGATGGAA 
AAGCTTCAGG CAGACAGTAA AACTGCACAG GAGGCATACG AGTCAACAGT GGAATACTTT 
GGGGAGAATC CCAAGACTAC TCCTCCAACT ATGTTCTTCC CTACATTTGT ACGCTTTATT 
AAAGCATACC AGAAAGCAGA GGAAGATATA AAAGTGTGGA AGAAGCAAGA AGCAGCTGCC 
AAAGAAGAGG AGCAAGTTTC CCCCGGCAAA GGGGAACAGG CAGTACCCAA GCAATCTCCC 
ACCCATAAGG CCAAGCGACA ACAGATGGAC CTCATAACTG AGCTGAAGAA AAAGCAGATG 
GTGAAAGAGC CTCTCATTTA TGAAGAAAAA GATGGTGCCA TTGAAGACAT TATTTCAGGT 
CTACGTATCC AGCCATACCG GCGGGGGGAC ACTGGTCGGC ACAGTGGCAA AAAACGAACC 
ACTGGGCAGA TTCTCCAGGC AACCTCCGAG ATCTCACTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FMNL1 ( XM_014058297.1 ) cDNA ORF clone, Thamnophis sirtalis -> XP_013913772.1 Thamnophis sirtalis formin-like 1 (FMNL1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OTh18451
Clone ID Related Accession (Same CDS sequence)	XM_014058297.1
Accession Version	XM_014058297.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3510bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-10
Organism	Thamnophis sirtalis
Product	formin-like protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_013658128.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Thamnophis sirtalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTAATG CAGCCAGCAG CCTGGAAATG TCTCCAGCGA AGGAGATTAA AACCCAACCG 
ACTGCTTCTT CGCCTTTACC CCCTAAACAG CCTGCACCCC CCAAACTACC GATGCCTAAT 
GCTGAGGAAT TGGAAGAACG ATTCGGACAT GTTCTGAATT CAATGAATCT GCCACCAGAC 
AAAATGAAGC TTCTGAGCCA GTATGATAAT GAAAAGAAAT GGGAATTAAT ATGTGACCAG 
GAACGATTCC AGGTCAAAAA CCCACCATCT GCCTATATCC AGAAACTGAA AAGCTATCTA 
GACACAGGTG GATTCAGTGG AAAGTTTAAG AGACGAGTTC AAGAATCTAC CCAGATTCTT 
CGGGAGTTAG AGATATCCCT GAGAACAAAC CACATTGGTT GGGTCCAGGA GTTCCTAAAT 
GAAGAGAATA GAGGTCTTGA TGTGTTGGTG GATTATCTTG CTTTTGCCCA GTGCTCTGTC 
ACGTATGATA TGGACAGCAC TGACAATGGG AGCCCTGGGT CGGACAAGGG AAAGGCCCTG 
GACAGATCTG TAGAGGACCT CAGCAGAAGC AATTGCTCTC CCACACAGGG TTCCATCAAA 
GCGAGGCACT TGACTGTCAG GTGTTGCCAT TTGAACCAGC AGATCCAAAC ATTCTTTCAA 
CAAGTCCACC CCAGCATCCC CTCCTCCAGC GCCCCTGGCA CCGAGCAGGA CTGTAAGCCC 
AGGCCACCTG TCAACTCTTA CTCACCCCGA TTCAATCCGG CACACAGCAG GAAGGCATTA 
CGGAACTCAC GCATCGTCAG CCAGAAGGAT GATGTTCATG TGTGCATCAT GTGTCTCCGT 
GCCATTATGA ATTATCAGTC TGGATTCAGC CTTGTGATGA ACCACCCTGC CTGTGTAAAT 
GAGATCACAC TCAGCCTCAA CAATAAGAAT CCCAGAACTA AAGCTCTTGT CCTTGAGCTA 
TTGGCTGCTG TTTGCTTGGT CCGAGGAGGA CACGACATTA TTCTGGCTGC CTTTGACAAC 
TTCAAAGAGG TTTGTGGTGA GAAGAACCGT TTTGAGAAGC TGATGGAGTA TTTCCGAAAT 
GAAGACAGCA ACATAGATTT CATGGTGGCT TGTATGCAGT TCATCAATAT TGTGGTTCAT 
TCTGTGGAGA ACATGAACTT CCGTGCCTTC TTACAATATG AGTTTACACA GTTGGGACTT 
GATGAGTACC TACAGAAGCT GCGTTGTACA GAAAGTGATA AACTGCAAGT GCAGATCCAA 
GCCTATCTGG ATAATATCTT TGATGTTGGT GCCTTACTGG AGGATACCGA GACCAAAAAT 
GCCATCCTGG AACACATAGA GGATTTGCAA GAAGAGGTTG GACAGCTCAC TGAGAAACTC 
CAGGATGCAG AAAACGAATC CGAAGCCAAA ATTGCTGAGC TAGAGAAACA GCTGTATCAG 
TCACGCAATG AGCTGGAGAT CCTGAGAGCA AGAACTAGTG ATGAGCCGGA GAAGCTAGAT 
GAAATCAGCA AAGTTAACAA GCCAGAAAGT CCTTCTTGGT TCCCAATGCA GCTTCCACCA 
GAGTCAAAAA TAACTCCACA ATACTCTGCC TTGGAGCACA ATCTGGAAGA ACTAGAAGAC 
AGAGGTTTTA TCCGGATCCA GCGTGGGCCT GGCAGTGATA GCATCATTGG CATTGAGGTC 
CTCCCTGTTA TTAATATTGA AGCATCTTCG TCAACTAGCA CAGATTATCC CTCCCCATCT 
ACATCTCCTG TCCGCAGTCT TGCTCCAGAA ATACCACCAG CACCACCTCT ACCTTCCACG 
AAAGGCAGTG ATTCTGGGGC TTTCTATTCT TCTCAAGAGG AGGGGTTGCC ACCTATTCCA 
ACTTCTCTTC CACCACCACC ACCACCACCC TTACCAGGGA TCAATGAGCC TCCACCTGCC 
CCACCACTGC CTGTGATTGT AACTACATCT CAAGCTTCAT CAACGCCTCC TCCTCCTCCG 
CCACCACCCC TCTTAACATT TGGTGGCCCA GTTGCTCCTC CTCCTCCACC ACCGCCTCCC 
ATGATTGATG GAGCAACTTT GCCAGCCAGT ACAGTTACTG CAGCTGTCAA GATCAAGAAG 
CCTATACAGA CCAAATTCCG TATGCCTGTC TTCAATTGGG TTGCTTTGAA GCCTAACCAG 
ATCAATGGGA CCATCTTCAA TGAACTCAAT GATGAAAAAG TCCTTCAGGA GCTGGACATG 
AATGACTTTG AAGAACAATT CAAAACCAAA GCTCAGGGTC CAGGCAAAGA ATTCAGTTCC 
ATAAAGACCA AGACTTTACA GAAAGCTCCC ACTAAGGTTT CACTGATTGA ATGCAACCGG 
GCCAAGAACC TGGCCATCAC CCTCCGTAAA GGGGGGCTCA GTATCGACAG CATTTGCAAA 
GCCATTCAGA CATACGACTT ACAAACCCTC AATCTGGATT TTCTGGAGTT ACTGATGCGC 
TTCATCCCAA CGGAATATGA GATGACACTA ATCCGCAAAT ATGAAAAAGA GCAACGTCCA 
CTGGATGAGC TTTCAGATGA GGACCAATTC ATGATCAAAT TCAGCAAAAT TCCCCGCCTT 
ACAGAACGCA TGAACATCAT GACATTCTTA GGCAGTTTTG CAGATACCGT ACAACTCCTT 
CTGCCACAAC TAAATTTTGT GATTGTTGCT TCAATGTCAG TCAAGTCATC TACCAAACTG 
CGTCAGATTT TAGAGATTGT CCTGGCATTT GGGAATTATT TGAACAGCAG CAAACGGGGA 
GCGGCTTATG GATTCCGGTT GCAGAGCCTT GATGCGCTTC TGGATATGAA ATCAACAGAC 
CGGAAACAGA CACTACTTCA TTATATAGTG CGGGTGATCC AAGAAAAATA CCCTGAGTTC 
CTGAACTTTT CCAGTGAGCT GCATTTTCTG GACAAAGCAG GGGCAGTGTC ACTGGACAGT 
GTGCTTCAAG ATGTGAGAAG CCTACAGCGG GGCATGGAGC TAACACGCAA AGAATTCATG 
CGCCAGGATG ACAGTATCAT ACTCAAGGAT TTCCTAAAGA CAAACATAGA AATGATGGAA 
AAGCTTCAGG CAGACAGTAA AACTGCACAG GAGGCATACG AGTCAACAGT GGAATACTTT 
GGGGAGAATC CCAAGACTAC TCCTCCAACT ATGTTCTTCC CTACATTTGT ACGCTTTATT 
AAAGCATACC AGAAAGCAGA GGAAGATATA AAAGTGTGGA AGAAGCAAGA AGCAGCTGCC 
AAAGAAGAGG AGCAAGTTTC CCCCGGCAAA GGGGAACAGG CAGTACCCAA GCAATCTCCC 
ACCCATAAGG CCAAGCGACA ACAGATGGAC CTCATAACTG AGCTGAAGAA AAAGCAGATG 
GTGAAAGAGC CTCTCATTTA TGAAGAAAAA GATGGTGCCA TTGAAGACAT TATTTCAGCT 
TTGAAAACTA TTCCTTTTAC TGCTCGGACG GGAAAACGCT CATCTCGCCT CTTCTGCGAT 
GCCAGCTTCA ATGAAGAAAG CCCACTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013913772.1
CDS	810..4319
Translation	MGNAASSLEMSPAKEIKTQPTASSPLPPKQPAPPKLPMPNAEELEERFGHVLNSMNLPPDKMKLLSQYDNEKKWELICDQERFQVKNPPSAYIQKLKSYLDTGGFSGKFKRRVQESTQILRELEISLRTNHIGWVQEFLNEENRGLDVLVDYLAFAQCSVTYDMDSTDNGSPGSDKGKALDRSVEDLSRSNCSPTQGSIKARHLTVRCCHLNQQIQTFFQQVHPSIPSSSAPGTEQDCKPRPPVNSYSPRFNPAHSRKALRNSRIVSQKDDVHVCIMCLRAIMNYQSGFSLVMNHPACVNEITLSLNNKNPRTKALVLELLAAVCLVRGGHDIILAAFDNFKEVCGEKNRFEKLMEYFRNEDSNIDFMVACMQFINIVVHSVENMNFRAFLQYEFTQLGLDEYLQKLRCTESDKLQVQIQAYLDNIFDVGALLEDTETKNAILEHIEDLQEEVGQLTEKLQDAENESEAKIAELEKQLYQSRNELEILRARTSDEPEKLDEISKVNKPESPSWFPMQLPPESKITPQYSALEHNLEELEDRGFIRIQRGPGSDSIIGIEVLPVINIEASSSTSTDYPSPSTSPVRSLAPEIPPAPPLPSTKGSDSGAFYSSQEEGLPPIPTSLPPPPPPPLPGINEPPPAPPLPVIVTTSQASSTPPPPPPPPLLTFGGPVAPPPPPPPPMIDGATLPASTVTAAVKIKKPIQTKFRMPVFNWVALKPNQINGTIFNELNDEKVLQELDMNDFEEQFKTKAQGPGKEFSSIKTKTLQKAPTKVSLIECNRAKNLAITLRKGGLSIDSICKAIQTYDLQTLNLDFLELLMRFIPTEYEMTLIRKYEKEQRPLDELSDEDQFMIKFSKIPRLTERMNIMTFLGSFADTVQLLLPQLNFVIVASMSVKSSTKLRQILEIVLAFGNYLNSSKRGAAYGFRLQSLDALLDMKSTDRKQTLLHYIVRVIQEKYPEFLNFSSELHFLDKAGAVSLDSVLQDVRSLQRGMELTRKEFMRQDDSIILKDFLKTNIEMMEKLQADSKTAQEAYESTVEYFGENPKTTPPTMFFPTFVRFIKAYQKAEEDIKVWKKQEAAAKEEEQVSPGKGEQAVPKQSPTHKAKRQQMDLITELKKKQMVKEPLIYEEKDGAIEDIISALKTIPFTARTGKRSSRLFCDASFNEESPL

Target ORF information:

RefSeq Version	XM_014058297.1
Organism	Thamnophis sirtalis
Definition	Thamnophis sirtalis formin-like 1 (FMNL1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014058297.1

ORF Insert Sequence:

ATGGGTAATG CAGCCAGCAG CCTGGAAATG TCTCCAGCGA AGGAGATTAA AACCCAACCG 
ACTGCTTCTT CGCCTTTACC CCCTAAACAG CCTGCACCCC CCAAACTACC GATGCCTAAT 
GCTGAGGAAT TGGAAGAACG ATTCGGACAT GTTCTGAATT CAATGAATCT GCCACCAGAC 
AAAATGAAGC TTCTGAGCCA GTATGATAAT GAAAAGAAAT GGGAATTAAT ATGTGACCAG 
GAACGATTCC AGGTCAAAAA CCCACCATCT GCCTATATCC AGAAACTGAA AAGCTATCTA 
GACACAGGTG GATTCAGTGG AAAGTTTAAG AGACGAGTTC AAGAATCTAC CCAGATTCTT 
CGGGAGTTAG AGATATCCCT GAGAACAAAC CACATTGGTT GGGTCCAGGA GTTCCTAAAT 
GAAGAGAATA GAGGTCTTGA TGTGTTGGTG GATTATCTTG CTTTTGCCCA GTGCTCTGTC 
ACGTATGATA TGGACAGCAC TGACAATGGG AGCCCTGGGT CGGACAAGGG AAAGGCCCTG 
GACAGATCTG TAGAGGACCT CAGCAGAAGC AATTGCTCTC CCACACAGGG TTCCATCAAA 
GCGAGGCACT TGACTGTCAG GTGTTGCCAT TTGAACCAGC AGATCCAAAC ATTCTTTCAA 
CAAGTCCACC CCAGCATCCC CTCCTCCAGC GCCCCTGGCA CCGAGCAGGA CTGTAAGCCC 
AGGCCACCTG TCAACTCTTA CTCACCCCGA TTCAATCCGG CACACAGCAG GAAGGCATTA 
CGGAACTCAC GCATCGTCAG CCAGAAGGAT GATGTTCATG TGTGCATCAT GTGTCTCCGT 
GCCATTATGA ATTATCAGTC TGGATTCAGC CTTGTGATGA ACCACCCTGC CTGTGTAAAT 
GAGATCACAC TCAGCCTCAA CAATAAGAAT CCCAGAACTA AAGCTCTTGT CCTTGAGCTA 
TTGGCTGCTG TTTGCTTGGT CCGAGGAGGA CACGACATTA TTCTGGCTGC CTTTGACAAC 
TTCAAAGAGG TTTGTGGTGA GAAGAACCGT TTTGAGAAGC TGATGGAGTA TTTCCGAAAT 
GAAGACAGCA ACATAGATTT CATGGTGGCT TGTATGCAGT TCATCAATAT TGTGGTTCAT 
TCTGTGGAGA ACATGAACTT CCGTGCCTTC TTACAATATG AGTTTACACA GTTGGGACTT 
GATGAGTACC TACAGAAGCT GCGTTGTACA GAAAGTGATA AACTGCAAGT GCAGATCCAA 
GCCTATCTGG ATAATATCTT TGATGTTGGT GCCTTACTGG AGGATACCGA GACCAAAAAT 
GCCATCCTGG AACACATAGA GGATTTGCAA GAAGAGGTTG GACAGCTCAC TGAGAAACTC 
CAGGATGCAG AAAACGAATC CGAAGCCAAA ATTGCTGAGC TAGAGAAACA GCTGTATCAG 
TCACGCAATG AGCTGGAGAT CCTGAGAGCA AGAACTAGTG ATGAGCCGGA GAAGCTAGAT 
GAAATCAGCA AAGTTAACAA GCCAGAAAGT CCTTCTTGGT TCCCAATGCA GCTTCCACCA 
GAGTCAAAAA TAACTCCACA ATACTCTGCC TTGGAGCACA ATCTGGAAGA ACTAGAAGAC 
AGAGGTTTTA TCCGGATCCA GCGTGGGCCT GGCAGTGATA GCATCATTGG CATTGAGGTC 
CTCCCTGTTA TTAATATTGA AGCATCTTCG TCAACTAGCA CAGATTATCC CTCCCCATCT 
ACATCTCCTG TCCGCAGTCT TGCTCCAGAA ATACCACCAG CACCACCTCT ACCTTCCACG 
AAAGGCAGTG ATTCTGGGGC TTTCTATTCT TCTCAAGAGG AGGGGTTGCC ACCTATTCCA 
ACTTCTCTTC CACCACCACC ACCACCACCC TTACCAGGGA TCAATGAGCC TCCACCTGCC 
CCACCACTGC CTGTGATTGT AACTACATCT CAAGCTTCAT CAACGCCTCC TCCTCCTCCG 
CCACCACCCC TCTTAACATT TGGTGGCCCA GTTGCTCCTC CTCCTCCACC ACCGCCTCCC 
ATGATTGATG GAGCAACTTT GCCAGCCAGT ACAGTTACTG CAGCTGTCAA GATCAAGAAG 
CCTATACAGA CCAAATTCCG TATGCCTGTC TTCAATTGGG TTGCTTTGAA GCCTAACCAG 
ATCAATGGGA CCATCTTCAA TGAACTCAAT GATGAAAAAG TCCTTCAGGA GCTGGACATG 
AATGACTTTG AAGAACAATT CAAAACCAAA GCTCAGGGTC CAGGCAAAGA ATTCAGTTCC 
ATAAAGACCA AGACTTTACA GAAAGCTCCC ACTAAGGTTT CACTGATTGA ATGCAACCGG 
GCCAAGAACC TGGCCATCAC CCTCCGTAAA GGGGGGCTCA GTATCGACAG CATTTGCAAA 
GCCATTCAGA CATACGACTT ACAAACCCTC AATCTGGATT TTCTGGAGTT ACTGATGCGC 
TTCATCCCAA CGGAATATGA GATGACACTA ATCCGCAAAT ATGAAAAAGA GCAACGTCCA 
CTGGATGAGC TTTCAGATGA GGACCAATTC ATGATCAAAT TCAGCAAAAT TCCCCGCCTT 
ACAGAACGCA TGAACATCAT GACATTCTTA GGCAGTTTTG CAGATACCGT ACAACTCCTT 
CTGCCACAAC TAAATTTTGT GATTGTTGCT TCAATGTCAG TCAAGTCATC TACCAAACTG 
CGTCAGATTT TAGAGATTGT CCTGGCATTT GGGAATTATT TGAACAGCAG CAAACGGGGA 
GCGGCTTATG GATTCCGGTT GCAGAGCCTT GATGCGCTTC TGGATATGAA ATCAACAGAC 
CGGAAACAGA CACTACTTCA TTATATAGTG CGGGTGATCC AAGAAAAATA CCCTGAGTTC 
CTGAACTTTT CCAGTGAGCT GCATTTTCTG GACAAAGCAG GGGCAGTGTC ACTGGACAGT 
GTGCTTCAAG ATGTGAGAAG CCTACAGCGG GGCATGGAGC TAACACGCAA AGAATTCATG 
CGCCAGGATG ACAGTATCAT ACTCAAGGAT TTCCTAAAGA CAAACATAGA AATGATGGAA 
AAGCTTCAGG CAGACAGTAA AACTGCACAG GAGGCATACG AGTCAACAGT GGAATACTTT 
GGGGAGAATC CCAAGACTAC TCCTCCAACT ATGTTCTTCC CTACATTTGT ACGCTTTATT 
AAAGCATACC AGAAAGCAGA GGAAGATATA AAAGTGTGGA AGAAGCAAGA AGCAGCTGCC 
AAAGAAGAGG AGCAAGTTTC CCCCGGCAAA GGGGAACAGG CAGTACCCAA GCAATCTCCC 
ACCCATAAGG CCAAGCGACA ACAGATGGAC CTCATAACTG AGCTGAAGAA AAAGCAGATG 
GTGAAAGAGC CTCTCATTTA TGAAGAAAAA GATGGTGCCA TTGAAGACAT TATTTCAGCT 
TTGAAAACTA TTCCTTTTAC TGCTCGGACG GGAAAACGCT CATCTCGCCT CTTCTGCGAT 
GCCAGCTTCA ATGAAGAAAG CCCACTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FMNL1 ( XM_014058298.1 ) cDNA ORF clone, Thamnophis sirtalis -> XP_013913773.1 Thamnophis sirtalis formin-like 1 (FMNL1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OTh18452
Clone ID Related Accession (Same CDS sequence)	XM_014058298.1
Accession Version	XM_014058298.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3453bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-10
Organism	Thamnophis sirtalis
Product	formin-like protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_013658128.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Thamnophis sirtalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTAATG CAGCCAGCAG CCTGGAAATG TCTCCAGCGA AGGAGATTAA AACCCAACCG 
ACTGCTTCTT CGCCTTTACC CCCTAAACAG CCTGCACCCC CCAAACTACC GATGCCTAAT 
GCTGAGGAAT TGGAAGAACG ATTCGGACAT GTTCTGAATT CAATGAATCT GCCACCAGAC 
AAAATGAAGC TTCTGAGCCA GTATGATAAT GAAAAGAAAT GGGAATTAAT ATGTGACCAG 
GAACGATTCC AGGTCAAAAA CCCACCATCT GCCTATATCC AGAAACTGAA AAGCTATCTA 
GACACAGGTG GATTCAGTGG AAAGTTTAAG AGACGAGTTC AAGAATCTAC CCAGATTCTT 
CGGGAGTTAG AGATATCCCT GAGAACAAAC CACATTGGTT GGGTCCAGGA GTTCCTAAAT 
GAAGAGAATA GAGGTCTTGA TGTGTTGGTG GATTATCTTG CTTTTGCCCA GTGCTCTGTC 
ACGTATGATA TGGACAGCAC TGACAATGGG AGCCCTGGGT CGGACAAGGG AAAGGCCCTG 
GACAGATCTG TAGAGGACCT CAGCAGAAGC AATTGCTCTC CCACACAGGG TTCCATCAAA 
GCGAGGCACT TGACTGTCAG GTGTTGCCAT TTGAACCAGC AGATCCAAAC ATTCTTTCAA 
CAAGTCCACC CCAGCATCCC CTCCTCCAGC GCCCCTGGCA CCGAGCAGGA CTGTAAGCCC 
AGGCCACCTG TCAACTCTTA CTCACCCCGA TTCAATCCGG CACACAGCAG GAAGGCATTA 
CGGAACTCAC GCATCGTCAG CCAGAAGGAT GATGTTCATG TGTGCATCAT GTGTCTCCGT 
GCCATTATGA ATTATCAGTC TGGATTCAGC CTTGTGATGA ACCACCCTGC CTGTGTAAAT 
GAGATCACAC TCAGCCTCAA CAATAAGAAT CCCAGAACTA AAGCTCTTGT CCTTGAGCTA 
TTGGCTGCTG TTTGCTTGGT CCGAGGAGGA CACGACATTA TTCTGGCTGC CTTTGACAAC 
TTCAAAGAGG TTTGTGGTGA GAAGAACCGT TTTGAGAAGC TGATGGAGTA TTTCCGAAAT 
GAAGACAGCA ACATAGATTT CATGGTGGCT TGTATGCAGT TCATCAATAT TGTGGTTCAT 
TCTGTGGAGA ACATGAACTT CCGTGCCTTC TTACAATATG AGTTTACACA GTTGGGACTT 
GATGAGTACC TACAGAAGCT GCGTTGTACA GAAAGTGATA AACTGCAAGT GCAGATCCAA 
GCCTATCTGG ATAATATCTT TGATGTTGGT GCCTTACTGG AGGATACCGA GACCAAAAAT 
GCCATCCTGG AACACATAGA GGATTTGCAA GAAGAGGTTG GACAGCTCAC TGAGAAACTC 
CAGGATGCAG AAAACGAATC CGAAGCCAAA ATTGCTGAGC TAGAGAAACA GCTGTATCAG 
TCACGCAATG AGCTGGAGAT CCTGAGAGCA AGAACTAGTG ATGAGCCGGA GAAGCTAGAT 
GAAATCAGCA AAGTTAACAA GCCAGAAAGT CCTTCTTGGT TCCCAATGCA GCTTCCACCA 
GAGTCAAAAA TAACTCCACA ATACTCTGCC TTGGAGCACA ATCTGGAAGA ACTAGAAGAC 
AGAGGTTTTA TCCGGATCCA GCGTGGGCCT GGCAGTGATA GCATCATTGG CATTGAGGTC 
CTCCCTGTTA TTAATATTGA AGCATCTTCG TCAACTAGCA CAGATTATCC CTCCCCATCT 
ACATCTCCTG TCCGCAGTCT TGCTCCAGAA ATACCACCAG CACCACCTCT ACCTTCCACG 
AAAGGCAGTG ATTCTGGGGC TTTCTATTCT TCTCAAGAGG AGGGGTTGCC ACCTATTCCA 
ACTTCTCTTC CACCACCACC ACCACCACCC TTACCAGGGA TCAATGAGCC TCCACCTGCC 
CCACCACTGC CTGTGATTGT AACTACATCT CAAGCTTCAT CAACGCCTCC TCCTCCTCCG 
CCACCACCCC TCTTAACATT TGGTGGCCCA GTTGCTCCTC CTCCTCCACC ACCGCCTCCC 
ATGATTGATG GAGCAACTTT GCCAGCCAGT ACAGTTACTG CAGCTGTCAA GATCAAGAAG 
CCTATACAGA CCAAATTCCG TATGCCTGTC TTCAATTGGG TTGCTTTGAA GCCTAACCAG 
ATCAATGGGA CCATCTTCAA TGAACTCAAT GATGAAAAAG TCCTTCAGGA GCTGGACATG 
AATGACTTTG AAGAACAATT CAAAACCAAA GCTCAGGGTC CAGGCAAAGA ATTCAGTTCC 
ATAAAGACCA AGACTTTACA GAAAGCTCCC ACTAAGGTTT CACTGATTGA ATGCAACCGG 
GCCAAGAACC TGGCCATCAC CCTCCGTAAA GGGGGGCTCA GTATCGACAG CATTTGCAAA 
GCCATTCAGA CATACGACTT ACAAACCCTC AATCTGGATT TTCTGGAGTT ACTGATGCGC 
TTCATCCCAA CGGAATATGA GATGACACTA ATCCGCAAAT ATGAAAAAGA GCAACGTCCA 
CTGGATGAGC TTTCAGATGA GGACCAATTC ATGATCAAAT TCAGCAAAAT TCCCCGCCTT 
ACAGAACGCA TGAACATCAT GACATTCTTA GGCAGTTTTG CAGATACCGT ACAACTCCTT 
CTGCCAATTG TCCTGGCATT TGGGAATTAT TTGAACAGCA GCAAACGGGG AGCGGCTTAT 
GGATTCCGGT TGCAGAGCCT TGATGCGCTT CTGGATATGA AATCAACAGA CCGGAAACAG 
ACACTACTTC ATTATATAGT GCGGGTGATC CAAGAAAAAT ACCCTGAGTT CCTGAACTTT 
TCCAGTGAGC TGCATTTTCT GGACAAAGCA GGGGCAGTGT CACTGGACAG TGTGCTTCAA 
GATGTGAGAA GCCTACAGCG GGGCATGGAG CTAACACGCA AAGAATTCAT GCGCCAGGAT 
GACAGTATCA TACTCAAGGA TTTCCTAAAG ACAAACATAG AAATGATGGA AAAGCTTCAG 
GCAGACAGTA AAACTGCACA GGAGGCATAC GAGTCAACAG TGGAATACTT TGGGGAGAAT 
CCCAAGACTA CTCCTCCAAC TATGTTCTTC CCTACATTTG TACGCTTTAT TAAAGCATAC 
CAGAAAGCAG AGGAAGATAT AAAAGTGTGG AAGAAGCAAG AAGCAGCTGC CAAAGAAGAG 
GAGCAAGTTT CCCCCGGCAA AGGGGAACAG GCAGTACCCA AGCAATCTCC CACCCATAAG 
GCCAAGCGAC AACAGATGGA CCTCATAACT GAGCTGAAGA AAAAGCAGAT GGTGAAAGAG 
CCTCTCATTT ATGAAGAAAA AGATGGTGCC ATTGAAGACA TTATTTCAGG TCTACGTATC 
CAGCCATACC GGCGGGGGGA CACTGGTCGG CACAGTGGCA AAAAACGAAC CACTGGGCAG 
ATTCTCCAGG CAACCTCCGA GATCTCACTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013913773.1
CDS	812..4264
Translation	MGNAASSLEMSPAKEIKTQPTASSPLPPKQPAPPKLPMPNAEELEERFGHVLNSMNLPPDKMKLLSQYDNEKKWELICDQERFQVKNPPSAYIQKLKSYLDTGGFSGKFKRRVQESTQILRELEISLRTNHIGWVQEFLNEENRGLDVLVDYLAFAQCSVTYDMDSTDNGSPGSDKGKALDRSVEDLSRSNCSPTQGSIKARHLTVRCCHLNQQIQTFFQQVHPSIPSSSAPGTEQDCKPRPPVNSYSPRFNPAHSRKALRNSRIVSQKDDVHVCIMCLRAIMNYQSGFSLVMNHPACVNEITLSLNNKNPRTKALVLELLAAVCLVRGGHDIILAAFDNFKEVCGEKNRFEKLMEYFRNEDSNIDFMVACMQFINIVVHSVENMNFRAFLQYEFTQLGLDEYLQKLRCTESDKLQVQIQAYLDNIFDVGALLEDTETKNAILEHIEDLQEEVGQLTEKLQDAENESEAKIAELEKQLYQSRNELEILRARTSDEPEKLDEISKVNKPESPSWFPMQLPPESKITPQYSALEHNLEELEDRGFIRIQRGPGSDSIIGIEVLPVINIEASSSTSTDYPSPSTSPVRSLAPEIPPAPPLPSTKGSDSGAFYSSQEEGLPPIPTSLPPPPPPPLPGINEPPPAPPLPVIVTTSQASSTPPPPPPPPLLTFGGPVAPPPPPPPPMIDGATLPASTVTAAVKIKKPIQTKFRMPVFNWVALKPNQINGTIFNELNDEKVLQELDMNDFEEQFKTKAQGPGKEFSSIKTKTLQKAPTKVSLIECNRAKNLAITLRKGGLSIDSICKAIQTYDLQTLNLDFLELLMRFIPTEYEMTLIRKYEKEQRPLDELSDEDQFMIKFSKIPRLTERMNIMTFLGSFADTVQLLLPIVLAFGNYLNSSKRGAAYGFRLQSLDALLDMKSTDRKQTLLHYIVRVIQEKYPEFLNFSSELHFLDKAGAVSLDSVLQDVRSLQRGMELTRKEFMRQDDSIILKDFLKTNIEMMEKLQADSKTAQEAYESTVEYFGENPKTTPPTMFFPTFVRFIKAYQKAEEDIKVWKKQEAAAKEEEQVSPGKGEQAVPKQSPTHKAKRQQMDLITELKKKQMVKEPLIYEEKDGAIEDIISGLRIQPYRRGDTGRHSGKKRTTGQILQATSEISL

Target ORF information:

RefSeq Version	XM_014058298.1
Organism	Thamnophis sirtalis
Definition	Thamnophis sirtalis formin-like 1 (FMNL1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014058298.1

ORF Insert Sequence:

ATGGGTAATG CAGCCAGCAG CCTGGAAATG TCTCCAGCGA AGGAGATTAA AACCCAACCG 
ACTGCTTCTT CGCCTTTACC CCCTAAACAG CCTGCACCCC CCAAACTACC GATGCCTAAT 
GCTGAGGAAT TGGAAGAACG ATTCGGACAT GTTCTGAATT CAATGAATCT GCCACCAGAC 
AAAATGAAGC TTCTGAGCCA GTATGATAAT GAAAAGAAAT GGGAATTAAT ATGTGACCAG 
GAACGATTCC AGGTCAAAAA CCCACCATCT GCCTATATCC AGAAACTGAA AAGCTATCTA 
GACACAGGTG GATTCAGTGG AAAGTTTAAG AGACGAGTTC AAGAATCTAC CCAGATTCTT 
CGGGAGTTAG AGATATCCCT GAGAACAAAC CACATTGGTT GGGTCCAGGA GTTCCTAAAT 
GAAGAGAATA GAGGTCTTGA TGTGTTGGTG GATTATCTTG CTTTTGCCCA GTGCTCTGTC 
ACGTATGATA TGGACAGCAC TGACAATGGG AGCCCTGGGT CGGACAAGGG AAAGGCCCTG 
GACAGATCTG TAGAGGACCT CAGCAGAAGC AATTGCTCTC CCACACAGGG TTCCATCAAA 
GCGAGGCACT TGACTGTCAG GTGTTGCCAT TTGAACCAGC AGATCCAAAC ATTCTTTCAA 
CAAGTCCACC CCAGCATCCC CTCCTCCAGC GCCCCTGGCA CCGAGCAGGA CTGTAAGCCC 
AGGCCACCTG TCAACTCTTA CTCACCCCGA TTCAATCCGG CACACAGCAG GAAGGCATTA 
CGGAACTCAC GCATCGTCAG CCAGAAGGAT GATGTTCATG TGTGCATCAT GTGTCTCCGT 
GCCATTATGA ATTATCAGTC TGGATTCAGC CTTGTGATGA ACCACCCTGC CTGTGTAAAT 
GAGATCACAC TCAGCCTCAA CAATAAGAAT CCCAGAACTA AAGCTCTTGT CCTTGAGCTA 
TTGGCTGCTG TTTGCTTGGT CCGAGGAGGA CACGACATTA TTCTGGCTGC CTTTGACAAC 
TTCAAAGAGG TTTGTGGTGA GAAGAACCGT TTTGAGAAGC TGATGGAGTA TTTCCGAAAT 
GAAGACAGCA ACATAGATTT CATGGTGGCT TGTATGCAGT TCATCAATAT TGTGGTTCAT 
TCTGTGGAGA ACATGAACTT CCGTGCCTTC TTACAATATG AGTTTACACA GTTGGGACTT 
GATGAGTACC TACAGAAGCT GCGTTGTACA GAAAGTGATA AACTGCAAGT GCAGATCCAA 
GCCTATCTGG ATAATATCTT TGATGTTGGT GCCTTACTGG AGGATACCGA GACCAAAAAT 
GCCATCCTGG AACACATAGA GGATTTGCAA GAAGAGGTTG GACAGCTCAC TGAGAAACTC 
CAGGATGCAG AAAACGAATC CGAAGCCAAA ATTGCTGAGC TAGAGAAACA GCTGTATCAG 
TCACGCAATG AGCTGGAGAT CCTGAGAGCA AGAACTAGTG ATGAGCCGGA GAAGCTAGAT 
GAAATCAGCA AAGTTAACAA GCCAGAAAGT CCTTCTTGGT TCCCAATGCA GCTTCCACCA 
GAGTCAAAAA TAACTCCACA ATACTCTGCC TTGGAGCACA ATCTGGAAGA ACTAGAAGAC 
AGAGGTTTTA TCCGGATCCA GCGTGGGCCT GGCAGTGATA GCATCATTGG CATTGAGGTC 
CTCCCTGTTA TTAATATTGA AGCATCTTCG TCAACTAGCA CAGATTATCC CTCCCCATCT 
ACATCTCCTG TCCGCAGTCT TGCTCCAGAA ATACCACCAG CACCACCTCT ACCTTCCACG 
AAAGGCAGTG ATTCTGGGGC TTTCTATTCT TCTCAAGAGG AGGGGTTGCC ACCTATTCCA 
ACTTCTCTTC CACCACCACC ACCACCACCC TTACCAGGGA TCAATGAGCC TCCACCTGCC 
CCACCACTGC CTGTGATTGT AACTACATCT CAAGCTTCAT CAACGCCTCC TCCTCCTCCG 
CCACCACCCC TCTTAACATT TGGTGGCCCA GTTGCTCCTC CTCCTCCACC ACCGCCTCCC 
ATGATTGATG GAGCAACTTT GCCAGCCAGT ACAGTTACTG CAGCTGTCAA GATCAAGAAG 
CCTATACAGA CCAAATTCCG TATGCCTGTC TTCAATTGGG TTGCTTTGAA GCCTAACCAG 
ATCAATGGGA CCATCTTCAA TGAACTCAAT GATGAAAAAG TCCTTCAGGA GCTGGACATG 
AATGACTTTG AAGAACAATT CAAAACCAAA GCTCAGGGTC CAGGCAAAGA ATTCAGTTCC 
ATAAAGACCA AGACTTTACA GAAAGCTCCC ACTAAGGTTT CACTGATTGA ATGCAACCGG 
GCCAAGAACC TGGCCATCAC CCTCCGTAAA GGGGGGCTCA GTATCGACAG CATTTGCAAA 
GCCATTCAGA CATACGACTT ACAAACCCTC AATCTGGATT TTCTGGAGTT ACTGATGCGC 
TTCATCCCAA CGGAATATGA GATGACACTA ATCCGCAAAT ATGAAAAAGA GCAACGTCCA 
CTGGATGAGC TTTCAGATGA GGACCAATTC ATGATCAAAT TCAGCAAAAT TCCCCGCCTT 
ACAGAACGCA TGAACATCAT GACATTCTTA GGCAGTTTTG CAGATACCGT ACAACTCCTT 
CTGCCAATTG TCCTGGCATT TGGGAATTAT TTGAACAGCA GCAAACGGGG AGCGGCTTAT 
GGATTCCGGT TGCAGAGCCT TGATGCGCTT CTGGATATGA AATCAACAGA CCGGAAACAG 
ACACTACTTC ATTATATAGT GCGGGTGATC CAAGAAAAAT ACCCTGAGTT CCTGAACTTT 
TCCAGTGAGC TGCATTTTCT GGACAAAGCA GGGGCAGTGT CACTGGACAG TGTGCTTCAA 
GATGTGAGAA GCCTACAGCG GGGCATGGAG CTAACACGCA AAGAATTCAT GCGCCAGGAT 
GACAGTATCA TACTCAAGGA TTTCCTAAAG ACAAACATAG AAATGATGGA AAAGCTTCAG 
GCAGACAGTA AAACTGCACA GGAGGCATAC GAGTCAACAG TGGAATACTT TGGGGAGAAT 
CCCAAGACTA CTCCTCCAAC TATGTTCTTC CCTACATTTG TACGCTTTAT TAAAGCATAC 
CAGAAAGCAG AGGAAGATAT AAAAGTGTGG AAGAAGCAAG AAGCAGCTGC CAAAGAAGAG 
GAGCAAGTTT CCCCCGGCAA AGGGGAACAG GCAGTACCCA AGCAATCTCC CACCCATAAG 
GCCAAGCGAC AACAGATGGA CCTCATAACT GAGCTGAAGA AAAAGCAGAT GGTGAAAGAG 
CCTCTCATTT ATGAAGAAAA AGATGGTGCC ATTGAAGACA TTATTTCAGG TCTACGTATC 
CAGCCATACC GGCGGGGGGA CACTGGTCGG CACAGTGGCA AAAAACGAAC CACTGGGCAG 
ATTCTCCAGG CAACCTCCGA GATCTCACTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FMNL1 ( XM_014058299.1 ) cDNA ORF clone, Thamnophis sirtalis -> XP_013913774.1 Thamnophis sirtalis formin-like 1 (FMNL1), transcript variant X4, mRNA.

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CloneID	OTh18453
Clone ID Related Accession (Same CDS sequence)	XM_014058299.1
Accession Version	XM_014058299.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3393bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-10
Organism	Thamnophis sirtalis
Product	formin-like protein 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_013658128.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Thamnophis sirtalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTAATG CAGCCAGCAG CCTGGAAATG TCTCCAGCGA AGGAGATTAA AACCCAACCG 
ACTGCTTCTT CGCCTTTACC CCCTAAACAG CCTGCACCCC CCAAACTACC GATGCCTAAT 
GCTGAGGAAT TGGAAGAACG ATTCGGACAT GTTCTGAATT CAATGAATCT GCCACCAGAC 
AAAATGAAGC TTCTGAGCCA GTATGATAAT GAAAAGAAAT GGGAATTAAT ATGTGACCAG 
GAACGATTCC AGGTCAAAAA CCCACCATCT GCCTATATCC AGAAACTGAA AAGCTATCTA 
GACACAGGTG GATTCAGTGG AAAGTTTAAG AGACGAGTTC AAGAATCTAC CCAGATTCTT 
CGGGAGTTAG AGATATCCCT GAGAACAAAC CACATTGGTT GGGTCCAGGA GTTCCTAAAT 
GAAGAGAATA GAGGTCTTGA TGTGTTGGTG GATTATCTTG CTTTTGCCCA GTGCTCTGTC 
ACGTATGATA TGGACAGCAC TGACAATGGG AGCCCTGGGT CGGACAAGGG AAAGGCCCTG 
GACAGATCTG TAGAGGACCT CAGCAGAAGC AATTGCTCTC CCACACAGGG TTCCATCAAA 
GCGAGGCACT TGACTGTCAG ATTCAATCCG GCACACAGCA GGAAGGCATT ACGGAACTCA 
CGCATCGTCA GCCAGAAGGA TGATGTTCAT GTGTGCATCA TGTGTCTCCG TGCCATTATG 
AATTATCAGT CTGGATTCAG CCTTGTGATG AACCACCCTG CCTGTGTAAA TGAGATCACA 
CTCAGCCTCA ACAATAAGAA TCCCAGAACT AAAGCTCTTG TCCTTGAGCT ATTGGCTGCT 
GTTTGCTTGG TCCGAGGAGG ACACGACATT ATTCTGGCTG CCTTTGACAA CTTCAAAGAG 
GTTTGTGGTG AGAAGAACCG TTTTGAGAAG CTGATGGAGT ATTTCCGAAA TGAAGACAGC 
AACATAGATT TCATGGTGGC TTGTATGCAG TTCATCAATA TTGTGGTTCA TTCTGTGGAG 
AACATGAACT TCCGTGCCTT CTTACAATAT GAGTTTACAC AGTTGGGACT TGATGAGTAC 
CTACAGAAGC TGCGTTGTAC AGAAAGTGAT AAACTGCAAG TGCAGATCCA AGCCTATCTG 
GATAATATCT TTGATGTTGG TGCCTTACTG GAGGATACCG AGACCAAAAA TGCCATCCTG 
GAACACATAG AGGATTTGCA AGAAGAGGTT GGACAGCTCA CTGAGAAACT CCAGGATGCA 
GAAAACGAAT CCGAAGCCAA AATTGCTGAG CTAGAGAAAC AGCTGTATCA GTCACGCAAT 
GAGCTGGAGA TCCTGAGAGC AAGAACTAGT GATGAGCCGG AGAAGCTAGA TGAAATCAGC 
AAAGTTAACA AGCCAGAAAG TCCTTCTTGG TTCCCAATGC AGCTTCCACC AGAGTCAAAA 
ATAACTCCAC AATACTCTGC CTTGGAGCAC AATCTGGAAG AACTAGAAGA CAGAGGTTTT 
ATCCGGATCC AGCGTGGGCC TGGCAGTGAT AGCATCATTG GCATTGAGGT CCTCCCTGTT 
ATTAATATTG AAGCATCTTC GTCAACTAGC ACAGATTATC CCTCCCCATC TACATCTCCT 
GTCCGCAGTC TTGCTCCAGA AATACCACCA GCACCACCTC TACCTTCCAC GAAAGGCAGT 
GATTCTGGGG CTTTCTATTC TTCTCAAGAG GAGGGGTTGC CACCTATTCC AACTTCTCTT 
CCACCACCAC CACCACCACC CTTACCAGGG ATCAATGAGC CTCCACCTGC CCCACCACTG 
CCTGTGATTG TAACTACATC TCAAGCTTCA TCAACGCCTC CTCCTCCTCC GCCACCACCC 
CTCTTAACAT TTGGTGGCCC AGTTGCTCCT CCTCCTCCAC CACCGCCTCC CATGATTGAT 
GGAGCAACTT TGCCAGCCAG TACAGTTACT GCAGCTGTCA AGATCAAGAA GCCTATACAG 
ACCAAATTCC GTATGCCTGT CTTCAATTGG GTTGCTTTGA AGCCTAACCA GATCAATGGG 
ACCATCTTCA ATGAACTCAA TGATGAAAAA GTCCTTCAGG AGCTGGACAT GAATGACTTT 
GAAGAACAAT TCAAAACCAA AGCTCAGGGT CCAGGCAAAG AATTCAGTTC CATAAAGACC 
AAGACTTTAC AGAAAGCTCC CACTAAGGTT TCACTGATTG AATGCAACCG GGCCAAGAAC 
CTGGCCATCA CCCTCCGTAA AGGGGGGCTC AGTATCGACA GCATTTGCAA AGCCATTCAG 
ACATACGACT TACAAACCCT CAATCTGGAT TTTCTGGAGT TACTGATGCG CTTCATCCCA 
ACGGAATATG AGATGACACT AATCCGCAAA TATGAAAAAG AGCAACGTCC ACTGGATGAG 
CTTTCAGATG AGGACCAATT CATGATCAAA TTCAGCAAAA TTCCCCGCCT TACAGAACGC 
ATGAACATCA TGACATTCTT AGGCAGTTTT GCAGATACCG TACAACTCCT TCTGCCACAA 
CTAAATTTTG TGATTGTTGC TTCAATGTCA GTCAAGTCAT CTACCAAACT GCGTCAGATT 
TTAGAGATTG TCCTGGCATT TGGGAATTAT TTGAACAGCA GCAAACGGGG AGCGGCTTAT 
GGATTCCGGT TGCAGAGCCT TGATGCGCTT CTGGATATGA AATCAACAGA CCGGAAACAG 
ACACTACTTC ATTATATAGT GCGGGTGATC CAAGAAAAAT ACCCTGAGTT CCTGAACTTT 
TCCAGTGAGC TGCATTTTCT GGACAAAGCA GGGGCAGTGT CACTGGACAG TGTGCTTCAA 
GATGTGAGAA GCCTACAGCG GGGCATGGAG CTAACACGCA AAGAATTCAT GCGCCAGGAT 
GACAGTATCA TACTCAAGGA TTTCCTAAAG ACAAACATAG AAATGATGGA AAAGCTTCAG 
GCAGACAGTA AAACTGCACA GGAGGCATAC GAGTCAACAG TGGAATACTT TGGGGAGAAT 
CCCAAGACTA CTCCTCCAAC TATGTTCTTC CCTACATTTG TACGCTTTAT TAAAGCATAC 
CAGAAAGCAG AGGAAGATAT AAAAGTGTGG AAGAAGCAAG AAGCAGCTGC CAAAGAAGAG 
GAGCAAGTTT CCCCCGGCAA AGGGGAACAG GCAGTACCCA AGCAATCTCC CACCCATAAG 
GCCAAGCGAC AACAGATGGA CCTCATAACT GAGCTGAAGA AAAAGCAGAT GGTGAAAGAG 
CCTCTCATTT ATGAAGAAAA AGATGGTGCC ATTGAAGACA TTATTTCAGG TCTACGTATC 
CAGCCATACC GGCGGGGGGA CACTGGTCGG CACAGTGGCA AAAAACGAAC CACTGGGCAG 
ATTCTCCAGG CAACCTCCGA GATCTCACTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013913774.1
CDS	810..4202
Translation	MGNAASSLEMSPAKEIKTQPTASSPLPPKQPAPPKLPMPNAEELEERFGHVLNSMNLPPDKMKLLSQYDNEKKWELICDQERFQVKNPPSAYIQKLKSYLDTGGFSGKFKRRVQESTQILRELEISLRTNHIGWVQEFLNEENRGLDVLVDYLAFAQCSVTYDMDSTDNGSPGSDKGKALDRSVEDLSRSNCSPTQGSIKARHLTVRFNPAHSRKALRNSRIVSQKDDVHVCIMCLRAIMNYQSGFSLVMNHPACVNEITLSLNNKNPRTKALVLELLAAVCLVRGGHDIILAAFDNFKEVCGEKNRFEKLMEYFRNEDSNIDFMVACMQFINIVVHSVENMNFRAFLQYEFTQLGLDEYLQKLRCTESDKLQVQIQAYLDNIFDVGALLEDTETKNAILEHIEDLQEEVGQLTEKLQDAENESEAKIAELEKQLYQSRNELEILRARTSDEPEKLDEISKVNKPESPSWFPMQLPPESKITPQYSALEHNLEELEDRGFIRIQRGPGSDSIIGIEVLPVINIEASSSTSTDYPSPSTSPVRSLAPEIPPAPPLPSTKGSDSGAFYSSQEEGLPPIPTSLPPPPPPPLPGINEPPPAPPLPVIVTTSQASSTPPPPPPPPLLTFGGPVAPPPPPPPPMIDGATLPASTVTAAVKIKKPIQTKFRMPVFNWVALKPNQINGTIFNELNDEKVLQELDMNDFEEQFKTKAQGPGKEFSSIKTKTLQKAPTKVSLIECNRAKNLAITLRKGGLSIDSICKAIQTYDLQTLNLDFLELLMRFIPTEYEMTLIRKYEKEQRPLDELSDEDQFMIKFSKIPRLTERMNIMTFLGSFADTVQLLLPQLNFVIVASMSVKSSTKLRQILEIVLAFGNYLNSSKRGAAYGFRLQSLDALLDMKSTDRKQTLLHYIVRVIQEKYPEFLNFSSELHFLDKAGAVSLDSVLQDVRSLQRGMELTRKEFMRQDDSIILKDFLKTNIEMMEKLQADSKTAQEAYESTVEYFGENPKTTPPTMFFPTFVRFIKAYQKAEEDIKVWKKQEAAAKEEEQVSPGKGEQAVPKQSPTHKAKRQQMDLITELKKKQMVKEPLIYEEKDGAIEDIISGLRIQPYRRGDTGRHSGKKRTTGQILQATSEISL

Target ORF information:

RefSeq Version	XM_014058299.1
Organism	Thamnophis sirtalis
Definition	Thamnophis sirtalis formin-like 1 (FMNL1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014058299.1

ORF Insert Sequence:

ATGGGTAATG CAGCCAGCAG CCTGGAAATG TCTCCAGCGA AGGAGATTAA AACCCAACCG 
ACTGCTTCTT CGCCTTTACC CCCTAAACAG CCTGCACCCC CCAAACTACC GATGCCTAAT 
GCTGAGGAAT TGGAAGAACG ATTCGGACAT GTTCTGAATT CAATGAATCT GCCACCAGAC 
AAAATGAAGC TTCTGAGCCA GTATGATAAT GAAAAGAAAT GGGAATTAAT ATGTGACCAG 
GAACGATTCC AGGTCAAAAA CCCACCATCT GCCTATATCC AGAAACTGAA AAGCTATCTA 
GACACAGGTG GATTCAGTGG AAAGTTTAAG AGACGAGTTC AAGAATCTAC CCAGATTCTT 
CGGGAGTTAG AGATATCCCT GAGAACAAAC CACATTGGTT GGGTCCAGGA GTTCCTAAAT 
GAAGAGAATA GAGGTCTTGA TGTGTTGGTG GATTATCTTG CTTTTGCCCA GTGCTCTGTC 
ACGTATGATA TGGACAGCAC TGACAATGGG AGCCCTGGGT CGGACAAGGG AAAGGCCCTG 
GACAGATCTG TAGAGGACCT CAGCAGAAGC AATTGCTCTC CCACACAGGG TTCCATCAAA 
GCGAGGCACT TGACTGTCAG ATTCAATCCG GCACACAGCA GGAAGGCATT ACGGAACTCA 
CGCATCGTCA GCCAGAAGGA TGATGTTCAT GTGTGCATCA TGTGTCTCCG TGCCATTATG 
AATTATCAGT CTGGATTCAG CCTTGTGATG AACCACCCTG CCTGTGTAAA TGAGATCACA 
CTCAGCCTCA ACAATAAGAA TCCCAGAACT AAAGCTCTTG TCCTTGAGCT ATTGGCTGCT 
GTTTGCTTGG TCCGAGGAGG ACACGACATT ATTCTGGCTG CCTTTGACAA CTTCAAAGAG 
GTTTGTGGTG AGAAGAACCG TTTTGAGAAG CTGATGGAGT ATTTCCGAAA TGAAGACAGC 
AACATAGATT TCATGGTGGC TTGTATGCAG TTCATCAATA TTGTGGTTCA TTCTGTGGAG 
AACATGAACT TCCGTGCCTT CTTACAATAT GAGTTTACAC AGTTGGGACT TGATGAGTAC 
CTACAGAAGC TGCGTTGTAC AGAAAGTGAT AAACTGCAAG TGCAGATCCA AGCCTATCTG 
GATAATATCT TTGATGTTGG TGCCTTACTG GAGGATACCG AGACCAAAAA TGCCATCCTG 
GAACACATAG AGGATTTGCA AGAAGAGGTT GGACAGCTCA CTGAGAAACT CCAGGATGCA 
GAAAACGAAT CCGAAGCCAA AATTGCTGAG CTAGAGAAAC AGCTGTATCA GTCACGCAAT 
GAGCTGGAGA TCCTGAGAGC AAGAACTAGT GATGAGCCGG AGAAGCTAGA TGAAATCAGC 
AAAGTTAACA AGCCAGAAAG TCCTTCTTGG TTCCCAATGC AGCTTCCACC AGAGTCAAAA 
ATAACTCCAC AATACTCTGC CTTGGAGCAC AATCTGGAAG AACTAGAAGA CAGAGGTTTT 
ATCCGGATCC AGCGTGGGCC TGGCAGTGAT AGCATCATTG GCATTGAGGT CCTCCCTGTT 
ATTAATATTG AAGCATCTTC GTCAACTAGC ACAGATTATC CCTCCCCATC TACATCTCCT 
GTCCGCAGTC TTGCTCCAGA AATACCACCA GCACCACCTC TACCTTCCAC GAAAGGCAGT 
GATTCTGGGG CTTTCTATTC TTCTCAAGAG GAGGGGTTGC CACCTATTCC AACTTCTCTT 
CCACCACCAC CACCACCACC CTTACCAGGG ATCAATGAGC CTCCACCTGC CCCACCACTG 
CCTGTGATTG TAACTACATC TCAAGCTTCA TCAACGCCTC CTCCTCCTCC GCCACCACCC 
CTCTTAACAT TTGGTGGCCC AGTTGCTCCT CCTCCTCCAC CACCGCCTCC CATGATTGAT 
GGAGCAACTT TGCCAGCCAG TACAGTTACT GCAGCTGTCA AGATCAAGAA GCCTATACAG 
ACCAAATTCC GTATGCCTGT CTTCAATTGG GTTGCTTTGA AGCCTAACCA GATCAATGGG 
ACCATCTTCA ATGAACTCAA TGATGAAAAA GTCCTTCAGG AGCTGGACAT GAATGACTTT 
GAAGAACAAT TCAAAACCAA AGCTCAGGGT CCAGGCAAAG AATTCAGTTC CATAAAGACC 
AAGACTTTAC AGAAAGCTCC CACTAAGGTT TCACTGATTG AATGCAACCG GGCCAAGAAC 
CTGGCCATCA CCCTCCGTAA AGGGGGGCTC AGTATCGACA GCATTTGCAA AGCCATTCAG 
ACATACGACT TACAAACCCT CAATCTGGAT TTTCTGGAGT TACTGATGCG CTTCATCCCA 
ACGGAATATG AGATGACACT AATCCGCAAA TATGAAAAAG AGCAACGTCC ACTGGATGAG 
CTTTCAGATG AGGACCAATT CATGATCAAA TTCAGCAAAA TTCCCCGCCT TACAGAACGC 
ATGAACATCA TGACATTCTT AGGCAGTTTT GCAGATACCG TACAACTCCT TCTGCCACAA 
CTAAATTTTG TGATTGTTGC TTCAATGTCA GTCAAGTCAT CTACCAAACT GCGTCAGATT 
TTAGAGATTG TCCTGGCATT TGGGAATTAT TTGAACAGCA GCAAACGGGG AGCGGCTTAT 
GGATTCCGGT TGCAGAGCCT TGATGCGCTT CTGGATATGA AATCAACAGA CCGGAAACAG 
ACACTACTTC ATTATATAGT GCGGGTGATC CAAGAAAAAT ACCCTGAGTT CCTGAACTTT 
TCCAGTGAGC TGCATTTTCT GGACAAAGCA GGGGCAGTGT CACTGGACAG TGTGCTTCAA 
GATGTGAGAA GCCTACAGCG GGGCATGGAG CTAACACGCA AAGAATTCAT GCGCCAGGAT 
GACAGTATCA TACTCAAGGA TTTCCTAAAG ACAAACATAG AAATGATGGA AAAGCTTCAG 
GCAGACAGTA AAACTGCACA GGAGGCATAC GAGTCAACAG TGGAATACTT TGGGGAGAAT 
CCCAAGACTA CTCCTCCAAC TATGTTCTTC CCTACATTTG TACGCTTTAT TAAAGCATAC 
CAGAAAGCAG AGGAAGATAT AAAAGTGTGG AAGAAGCAAG AAGCAGCTGC CAAAGAAGAG 
GAGCAAGTTT CCCCCGGCAA AGGGGAACAG GCAGTACCCA AGCAATCTCC CACCCATAAG 
GCCAAGCGAC AACAGATGGA CCTCATAACT GAGCTGAAGA AAAAGCAGAT GGTGAAAGAG 
CCTCTCATTT ATGAAGAAAA AGATGGTGCC ATTGAAGACA TTATTTCAGG TCTACGTATC 
CAGCCATACC GGCGGGGGGA CACTGGTCGG CACAGTGGCA AAAAACGAAC CACTGGGCAG 
ATTCTCCAGG CAACCTCCGA GATCTCACTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FMNL1 ( XM_014058300.1 ) cDNA ORF clone, Thamnophis sirtalis -> XP_013913775.1 Thamnophis sirtalis formin-like 1 (FMNL1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OTh18454
Clone ID Related Accession (Same CDS sequence)	XM_014058300.1
Accession Version	XM_014058300.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3372bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-10
Organism	Thamnophis sirtalis
Product	formin-like protein 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_013658128.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Thamnophis sirtalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTAATG CAGCCAGCAG CCTGGAAATG TCTCCAGCGA AGGAGATTAA AACCCAACCG 
ACTGCTTCTT CGCCTTTACC CCCTAAACAG CCTGCACCCC CCAAACTACC GATGCCTAAT 
GCTGAGGAAT TGGAAGAACG ATTCGGACAT GTTCTGAATT CAATGAATCT GCCACCAGAC 
AAAATGAAGC TTCTGAGCCA GTATGATAAT GAAAAGAAAT GGGAATTAAT ATGTGACCAG 
GAACGATTCC AGGTCAAAAA CCCACCATCT GCCTATATCC AGAAACTGAA AAGCTATCTA 
GACACAGGTG GATTCAGTGG AAAGTTTAAG AGACGAGTTC AAGAATCTAC CCAGATTCTT 
CGGGAGTTAG AGATATCCCT GAGAACAAAC CACATTGGTT GGGTCCAGGA GTTCCTAAAT 
GAAGAGAATA GAGGTCTTGA TGTGTTGGTG GATTATCTTG CTTTTGCCCA GTGCTCTGTC 
ACGTATGATA TGGACAGCAC TGACAATGGG AGCCCTGGGT CGGACAAGGG AAAGGCCCTG 
GACAGATCTG TAGAGGACCT CAGCAGAAGC AATTGCTCTC CCACACAGGG TTCCATCAAA 
GCGAGGCACT TGACTGTCAG GAAGGCATTA CGGAACTCAC GCATCGTCAG CCAGAAGGAT 
GATGTTCATG TGTGCATCAT GTGTCTCCGT GCCATTATGA ATTATCAGTC TGGATTCAGC 
CTTGTGATGA ACCACCCTGC CTGTGTAAAT GAGATCACAC TCAGCCTCAA CAATAAGAAT 
CCCAGAACTA AAGCTCTTGT CCTTGAGCTA TTGGCTGCTG TTTGCTTGGT CCGAGGAGGA 
CACGACATTA TTCTGGCTGC CTTTGACAAC TTCAAAGAGG TTTGTGGTGA GAAGAACCGT 
TTTGAGAAGC TGATGGAGTA TTTCCGAAAT GAAGACAGCA ACATAGATTT CATGGTGGCT 
TGTATGCAGT TCATCAATAT TGTGGTTCAT TCTGTGGAGA ACATGAACTT CCGTGCCTTC 
TTACAATATG AGTTTACACA GTTGGGACTT GATGAGTACC TACAGAAGCT GCGTTGTACA 
GAAAGTGATA AACTGCAAGT GCAGATCCAA GCCTATCTGG ATAATATCTT TGATGTTGGT 
GCCTTACTGG AGGATACCGA GACCAAAAAT GCCATCCTGG AACACATAGA GGATTTGCAA 
GAAGAGGTTG GACAGCTCAC TGAGAAACTC CAGGATGCAG AAAACGAATC CGAAGCCAAA 
ATTGCTGAGC TAGAGAAACA GCTGTATCAG TCACGCAATG AGCTGGAGAT CCTGAGAGCA 
AGAACTAGTG ATGAGCCGGA GAAGCTAGAT GAAATCAGCA AAGTTAACAA GCCAGAAAGT 
CCTTCTTGGT TCCCAATGCA GCTTCCACCA GAGTCAAAAA TAACTCCACA ATACTCTGCC 
TTGGAGCACA ATCTGGAAGA ACTAGAAGAC AGAGGTTTTA TCCGGATCCA GCGTGGGCCT 
GGCAGTGATA GCATCATTGG CATTGAGGTC CTCCCTGTTA TTAATATTGA AGCATCTTCG 
TCAACTAGCA CAGATTATCC CTCCCCATCT ACATCTCCTG TCCGCAGTCT TGCTCCAGAA 
ATACCACCAG CACCACCTCT ACCTTCCACG AAAGGCAGTG ATTCTGGGGC TTTCTATTCT 
TCTCAAGAGG AGGGGTTGCC ACCTATTCCA ACTTCTCTTC CACCACCACC ACCACCACCC 
TTACCAGGGA TCAATGAGCC TCCACCTGCC CCACCACTGC CTGTGATTGT AACTACATCT 
CAAGCTTCAT CAACGCCTCC TCCTCCTCCG CCACCACCCC TCTTAACATT TGGTGGCCCA 
GTTGCTCCTC CTCCTCCACC ACCGCCTCCC ATGATTGATG GAGCAACTTT GCCAGCCAGT 
ACAGTTACTG CAGCTGTCAA GATCAAGAAG CCTATACAGA CCAAATTCCG TATGCCTGTC 
TTCAATTGGG TTGCTTTGAA GCCTAACCAG ATCAATGGGA CCATCTTCAA TGAACTCAAT 
GATGAAAAAG TCCTTCAGGA GCTGGACATG AATGACTTTG AAGAACAATT CAAAACCAAA 
GCTCAGGGTC CAGGCAAAGA ATTCAGTTCC ATAAAGACCA AGACTTTACA GAAAGCTCCC 
ACTAAGGTTT CACTGATTGA ATGCAACCGG GCCAAGAACC TGGCCATCAC CCTCCGTAAA 
GGGGGGCTCA GTATCGACAG CATTTGCAAA GCCATTCAGA CATACGACTT ACAAACCCTC 
AATCTGGATT TTCTGGAGTT ACTGATGCGC TTCATCCCAA CGGAATATGA GATGACACTA 
ATCCGCAAAT ATGAAAAAGA GCAACGTCCA CTGGATGAGC TTTCAGATGA GGACCAATTC 
ATGATCAAAT TCAGCAAAAT TCCCCGCCTT ACAGAACGCA TGAACATCAT GACATTCTTA 
GGCAGTTTTG CAGATACCGT ACAACTCCTT CTGCCACAAC TAAATTTTGT GATTGTTGCT 
TCAATGTCAG TCAAGTCATC TACCAAACTG CGTCAGATTT TAGAGATTGT CCTGGCATTT 
GGGAATTATT TGAACAGCAG CAAACGGGGA GCGGCTTATG GATTCCGGTT GCAGAGCCTT 
GATGCGCTTC TGGATATGAA ATCAACAGAC CGGAAACAGA CACTACTTCA TTATATAGTG 
CGGGTGATCC AAGAAAAATA CCCTGAGTTC CTGAACTTTT CCAGTGAGCT GCATTTTCTG 
GACAAAGCAG GGGCAGTGTC ACTGGACAGT GTGCTTCAAG ATGTGAGAAG CCTACAGCGG 
GGCATGGAGC TAACACGCAA AGAATTCATG CGCCAGGATG ACAGTATCAT ACTCAAGGAT 
TTCCTAAAGA CAAACATAGA AATGATGGAA AAGCTTCAGG CAGACAGTAA AACTGCACAG 
GAGGCATACG AGTCAACAGT GGAATACTTT GGGGAGAATC CCAAGACTAC TCCTCCAACT 
ATGTTCTTCC CTACATTTGT ACGCTTTATT AAAGCATACC AGAAAGCAGA GGAAGATATA 
AAAGTGTGGA AGAAGCAAGA AGCAGCTGCC AAAGAAGAGG AGCAAGTTTC CCCCGGCAAA 
GGGGAACAGG CAGTACCCAA GCAATCTCCC ACCCATAAGG CCAAGCGACA ACAGATGGAC 
CTCATAACTG AGCTGAAGAA AAAGCAGATG GTGAAAGAGC CTCTCATTTA TGAAGAAAAA 
GATGGTGCCA TTGAAGACAT TATTTCAGGT CTACGTATCC AGCCATACCG GCGGGGGGAC 
ACTGGTCGGC ACAGTGGCAA AAAACGAACC ACTGGGCAGA TTCTCCAGGC AACCTCCGAG 
ATCTCACTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013913775.1
CDS	810..4181
Translation	MGNAASSLEMSPAKEIKTQPTASSPLPPKQPAPPKLPMPNAEELEERFGHVLNSMNLPPDKMKLLSQYDNEKKWELICDQERFQVKNPPSAYIQKLKSYLDTGGFSGKFKRRVQESTQILRELEISLRTNHIGWVQEFLNEENRGLDVLVDYLAFAQCSVTYDMDSTDNGSPGSDKGKALDRSVEDLSRSNCSPTQGSIKARHLTVRKALRNSRIVSQKDDVHVCIMCLRAIMNYQSGFSLVMNHPACVNEITLSLNNKNPRTKALVLELLAAVCLVRGGHDIILAAFDNFKEVCGEKNRFEKLMEYFRNEDSNIDFMVACMQFINIVVHSVENMNFRAFLQYEFTQLGLDEYLQKLRCTESDKLQVQIQAYLDNIFDVGALLEDTETKNAILEHIEDLQEEVGQLTEKLQDAENESEAKIAELEKQLYQSRNELEILRARTSDEPEKLDEISKVNKPESPSWFPMQLPPESKITPQYSALEHNLEELEDRGFIRIQRGPGSDSIIGIEVLPVINIEASSSTSTDYPSPSTSPVRSLAPEIPPAPPLPSTKGSDSGAFYSSQEEGLPPIPTSLPPPPPPPLPGINEPPPAPPLPVIVTTSQASSTPPPPPPPPLLTFGGPVAPPPPPPPPMIDGATLPASTVTAAVKIKKPIQTKFRMPVFNWVALKPNQINGTIFNELNDEKVLQELDMNDFEEQFKTKAQGPGKEFSSIKTKTLQKAPTKVSLIECNRAKNLAITLRKGGLSIDSICKAIQTYDLQTLNLDFLELLMRFIPTEYEMTLIRKYEKEQRPLDELSDEDQFMIKFSKIPRLTERMNIMTFLGSFADTVQLLLPQLNFVIVASMSVKSSTKLRQILEIVLAFGNYLNSSKRGAAYGFRLQSLDALLDMKSTDRKQTLLHYIVRVIQEKYPEFLNFSSELHFLDKAGAVSLDSVLQDVRSLQRGMELTRKEFMRQDDSIILKDFLKTNIEMMEKLQADSKTAQEAYESTVEYFGENPKTTPPTMFFPTFVRFIKAYQKAEEDIKVWKKQEAAAKEEEQVSPGKGEQAVPKQSPTHKAKRQQMDLITELKKKQMVKEPLIYEEKDGAIEDIISGLRIQPYRRGDTGRHSGKKRTTGQILQATSEISL

Target ORF information:

RefSeq Version	XM_014058300.1
Organism	Thamnophis sirtalis
Definition	Thamnophis sirtalis formin-like 1 (FMNL1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014058300.1

ORF Insert Sequence:

ATGGGTAATG CAGCCAGCAG CCTGGAAATG TCTCCAGCGA AGGAGATTAA AACCCAACCG 
ACTGCTTCTT CGCCTTTACC CCCTAAACAG CCTGCACCCC CCAAACTACC GATGCCTAAT 
GCTGAGGAAT TGGAAGAACG ATTCGGACAT GTTCTGAATT CAATGAATCT GCCACCAGAC 
AAAATGAAGC TTCTGAGCCA GTATGATAAT GAAAAGAAAT GGGAATTAAT ATGTGACCAG 
GAACGATTCC AGGTCAAAAA CCCACCATCT GCCTATATCC AGAAACTGAA AAGCTATCTA 
GACACAGGTG GATTCAGTGG AAAGTTTAAG AGACGAGTTC AAGAATCTAC CCAGATTCTT 
CGGGAGTTAG AGATATCCCT GAGAACAAAC CACATTGGTT GGGTCCAGGA GTTCCTAAAT 
GAAGAGAATA GAGGTCTTGA TGTGTTGGTG GATTATCTTG CTTTTGCCCA GTGCTCTGTC 
ACGTATGATA TGGACAGCAC TGACAATGGG AGCCCTGGGT CGGACAAGGG AAAGGCCCTG 
GACAGATCTG TAGAGGACCT CAGCAGAAGC AATTGCTCTC CCACACAGGG TTCCATCAAA 
GCGAGGCACT TGACTGTCAG GAAGGCATTA CGGAACTCAC GCATCGTCAG CCAGAAGGAT 
GATGTTCATG TGTGCATCAT GTGTCTCCGT GCCATTATGA ATTATCAGTC TGGATTCAGC 
CTTGTGATGA ACCACCCTGC CTGTGTAAAT GAGATCACAC TCAGCCTCAA CAATAAGAAT 
CCCAGAACTA AAGCTCTTGT CCTTGAGCTA TTGGCTGCTG TTTGCTTGGT CCGAGGAGGA 
CACGACATTA TTCTGGCTGC CTTTGACAAC TTCAAAGAGG TTTGTGGTGA GAAGAACCGT 
TTTGAGAAGC TGATGGAGTA TTTCCGAAAT GAAGACAGCA ACATAGATTT CATGGTGGCT 
TGTATGCAGT TCATCAATAT TGTGGTTCAT TCTGTGGAGA ACATGAACTT CCGTGCCTTC 
TTACAATATG AGTTTACACA GTTGGGACTT GATGAGTACC TACAGAAGCT GCGTTGTACA 
GAAAGTGATA AACTGCAAGT GCAGATCCAA GCCTATCTGG ATAATATCTT TGATGTTGGT 
GCCTTACTGG AGGATACCGA GACCAAAAAT GCCATCCTGG AACACATAGA GGATTTGCAA 
GAAGAGGTTG GACAGCTCAC TGAGAAACTC CAGGATGCAG AAAACGAATC CGAAGCCAAA 
ATTGCTGAGC TAGAGAAACA GCTGTATCAG TCACGCAATG AGCTGGAGAT CCTGAGAGCA 
AGAACTAGTG ATGAGCCGGA GAAGCTAGAT GAAATCAGCA AAGTTAACAA GCCAGAAAGT 
CCTTCTTGGT TCCCAATGCA GCTTCCACCA GAGTCAAAAA TAACTCCACA ATACTCTGCC 
TTGGAGCACA ATCTGGAAGA ACTAGAAGAC AGAGGTTTTA TCCGGATCCA GCGTGGGCCT 
GGCAGTGATA GCATCATTGG CATTGAGGTC CTCCCTGTTA TTAATATTGA AGCATCTTCG 
TCAACTAGCA CAGATTATCC CTCCCCATCT ACATCTCCTG TCCGCAGTCT TGCTCCAGAA 
ATACCACCAG CACCACCTCT ACCTTCCACG AAAGGCAGTG ATTCTGGGGC TTTCTATTCT 
TCTCAAGAGG AGGGGTTGCC ACCTATTCCA ACTTCTCTTC CACCACCACC ACCACCACCC 
TTACCAGGGA TCAATGAGCC TCCACCTGCC CCACCACTGC CTGTGATTGT AACTACATCT 
CAAGCTTCAT CAACGCCTCC TCCTCCTCCG CCACCACCCC TCTTAACATT TGGTGGCCCA 
GTTGCTCCTC CTCCTCCACC ACCGCCTCCC ATGATTGATG GAGCAACTTT GCCAGCCAGT 
ACAGTTACTG CAGCTGTCAA GATCAAGAAG CCTATACAGA CCAAATTCCG TATGCCTGTC 
TTCAATTGGG TTGCTTTGAA GCCTAACCAG ATCAATGGGA CCATCTTCAA TGAACTCAAT 
GATGAAAAAG TCCTTCAGGA GCTGGACATG AATGACTTTG AAGAACAATT CAAAACCAAA 
GCTCAGGGTC CAGGCAAAGA ATTCAGTTCC ATAAAGACCA AGACTTTACA GAAAGCTCCC 
ACTAAGGTTT CACTGATTGA ATGCAACCGG GCCAAGAACC TGGCCATCAC CCTCCGTAAA 
GGGGGGCTCA GTATCGACAG CATTTGCAAA GCCATTCAGA CATACGACTT ACAAACCCTC 
AATCTGGATT TTCTGGAGTT ACTGATGCGC TTCATCCCAA CGGAATATGA GATGACACTA 
ATCCGCAAAT ATGAAAAAGA GCAACGTCCA CTGGATGAGC TTTCAGATGA GGACCAATTC 
ATGATCAAAT TCAGCAAAAT TCCCCGCCTT ACAGAACGCA TGAACATCAT GACATTCTTA 
GGCAGTTTTG CAGATACCGT ACAACTCCTT CTGCCACAAC TAAATTTTGT GATTGTTGCT 
TCAATGTCAG TCAAGTCATC TACCAAACTG CGTCAGATTT TAGAGATTGT CCTGGCATTT 
GGGAATTATT TGAACAGCAG CAAACGGGGA GCGGCTTATG GATTCCGGTT GCAGAGCCTT 
GATGCGCTTC TGGATATGAA ATCAACAGAC CGGAAACAGA CACTACTTCA TTATATAGTG 
CGGGTGATCC AAGAAAAATA CCCTGAGTTC CTGAACTTTT CCAGTGAGCT GCATTTTCTG 
GACAAAGCAG GGGCAGTGTC ACTGGACAGT GTGCTTCAAG ATGTGAGAAG CCTACAGCGG 
GGCATGGAGC TAACACGCAA AGAATTCATG CGCCAGGATG ACAGTATCAT ACTCAAGGAT 
TTCCTAAAGA CAAACATAGA AATGATGGAA AAGCTTCAGG CAGACAGTAA AACTGCACAG 
GAGGCATACG AGTCAACAGT GGAATACTTT GGGGAGAATC CCAAGACTAC TCCTCCAACT 
ATGTTCTTCC CTACATTTGT ACGCTTTATT AAAGCATACC AGAAAGCAGA GGAAGATATA 
AAAGTGTGGA AGAAGCAAGA AGCAGCTGCC AAAGAAGAGG AGCAAGTTTC CCCCGGCAAA 
GGGGAACAGG CAGTACCCAA GCAATCTCCC ACCCATAAGG CCAAGCGACA ACAGATGGAC 
CTCATAACTG AGCTGAAGAA AAAGCAGATG GTGAAAGAGC CTCTCATTTA TGAAGAAAAA 
GATGGTGCCA TTGAAGACAT TATTTCAGGT CTACGTATCC AGCCATACCG GCGGGGGGAC 
ACTGGTCGGC ACAGTGGCAA AAAACGAACC ACTGGGCAGA TTCTCCAGGC AACCTCCGAG 
ATCTCACTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FMNL1 ( XM_014058301.1 ) cDNA ORF clone, Thamnophis sirtalis -> XP_013913776.1 Thamnophis sirtalis formin-like 1 (FMNL1), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OTh18455
Clone ID Related Accession (Same CDS sequence)	XM_014058301.1
Accession Version	XM_014058301.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3255bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-10
Organism	Thamnophis sirtalis
Product	formin-like protein 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_013658128.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Thamnophis sirtalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTAATG CAGCCAGCAG CCTGGAAATG TCTCCAGCGA AGGAGATTAA AACCCAACCG 
ACTGCTTCTT CGCCTTTACC CCCTAAACAG CCTGCACCCC CCAAACTACC GATGCCTAAT 
GCTGAGGAAT TGGAAGAACG ATTCGGACAT GTTCTGAATT CAATGAATCT GCCACCAGAC 
AAAATGAAGC TTCTGAGCCA GTATGATAAT GAAAAGAAAT GGGAATTAAT ATGTGACCAG 
GAACGATTCC AGGTCAAAAA CCCACCATCT GCCTATATCC AGAAACTGAA AAGCTATCTA 
GACACAGGTG GATTCAGTGG AAAGTTTAAG AGACGAGTTC AAGAATCTAC CCAGATTCTT 
CGGGAGTTAG AGATATCCCT GAGAACAAAC CACATTGGTT GGGTCCAGGA GTTCCTAAAT 
GAAGAGAATA GAGGTCTTGA TGTGTTGGTG GATTATCTTG CTTTTGCCCA GTGCTCTGTC 
ACATTCAATC CGGCACACAG CAGGAAGGCA TTACGGAACT CACGCATCGT CAGCCAGAAG 
GATGATGTTC ATGTGTGCAT CATGTGTCTC CGTGCCATTA TGAATTATCA GTCTGGATTC 
AGCCTTGTGA TGAACCACCC TGCCTGTGTA AATGAGATCA CACTCAGCCT CAACAATAAG 
AATCCCAGAA CTAAAGCTCT TGTCCTTGAG CTATTGGCTG CTGTTTGCTT GGTCCGAGGA 
GGACACGACA TTATTCTGGC TGCCTTTGAC AACTTCAAAG AGGTTTGTGG TGAGAAGAAC 
CGTTTTGAGA AGCTGATGGA GTATTTCCGA AATGAAGACA GCAACATAGA TTTCATGGTG 
GCTTGTATGC AGTTCATCAA TATTGTGGTT CATTCTGTGG AGAACATGAA CTTCCGTGCC 
TTCTTACAAT ATGAGTTTAC ACAGTTGGGA CTTGATGAGT ACCTACAGAA GCTGCGTTGT 
ACAGAAAGTG ATAAACTGCA AGTGCAGATC CAAGCCTATC TGGATAATAT CTTTGATGTT 
GGTGCCTTAC TGGAGGATAC CGAGACCAAA AATGCCATCC TGGAACACAT AGAGGATTTG 
CAAGAAGAGG TTGGACAGCT CACTGAGAAA CTCCAGGATG CAGAAAACGA ATCCGAAGCC 
AAAATTGCTG AGCTAGAGAA ACAGCTGTAT CAGTCACGCA ATGAGCTGGA GATCCTGAGA 
GCAAGAACTA GTGATGAGCC GGAGAAGCTA GATGAAATCA GCAAAGTTAA CAAGCCAGAA 
AGTCCTTCTT GGTTCCCAAT GCAGCTTCCA CCAGAGTCAA AAATAACTCC ACAATACTCT 
GCCTTGGAGC ACAATCTGGA AGAACTAGAA GACAGAGGTT TTATCCGGAT CCAGCGTGGG 
CCTGGCAGTG ATAGCATCAT TGGCATTGAG GTCCTCCCTG TTATTAATAT TGAAGCATCT 
TCGTCAACTA GCACAGATTA TCCCTCCCCA TCTACATCTC CTGTCCGCAG TCTTGCTCCA 
GAAATACCAC CAGCACCACC TCTACCTTCC ACGAAAGGCA GTGATTCTGG GGCTTTCTAT 
TCTTCTCAAG AGGAGGGGTT GCCACCTATT CCAACTTCTC TTCCACCACC ACCACCACCA 
CCCTTACCAG GGATCAATGA GCCTCCACCT GCCCCACCAC TGCCTGTGAT TGTAACTACA 
TCTCAAGCTT CATCAACGCC TCCTCCTCCT CCGCCACCAC CCCTCTTAAC ATTTGGTGGC 
CCAGTTGCTC CTCCTCCTCC ACCACCGCCT CCCATGATTG ATGGAGCAAC TTTGCCAGCC 
AGTACAGTTA CTGCAGCTGT CAAGATCAAG AAGCCTATAC AGACCAAATT CCGTATGCCT 
GTCTTCAATT GGGTTGCTTT GAAGCCTAAC CAGATCAATG GGACCATCTT CAATGAACTC 
AATGATGAAA AAGTCCTTCA GGAGCTGGAC ATGAATGACT TTGAAGAACA ATTCAAAACC 
AAAGCTCAGG GTCCAGGCAA AGAATTCAGT TCCATAAAGA CCAAGACTTT ACAGAAAGCT 
CCCACTAAGG TTTCACTGAT TGAATGCAAC CGGGCCAAGA ACCTGGCCAT CACCCTCCGT 
AAAGGGGGGC TCAGTATCGA CAGCATTTGC AAAGCCATTC AGACATACGA CTTACAAACC 
CTCAATCTGG ATTTTCTGGA GTTACTGATG CGCTTCATCC CAACGGAATA TGAGATGACA 
CTAATCCGCA AATATGAAAA AGAGCAACGT CCACTGGATG AGCTTTCAGA TGAGGACCAA 
TTCATGATCA AATTCAGCAA AATTCCCCGC CTTACAGAAC GCATGAACAT CATGACATTC 
TTAGGCAGTT TTGCAGATAC CGTACAACTC CTTCTGCCAC AACTAAATTT TGTGATTGTT 
GCTTCAATGT CAGTCAAGTC ATCTACCAAA CTGCGTCAGA TTTTAGAGAT TGTCCTGGCA 
TTTGGGAATT ATTTGAACAG CAGCAAACGG GGAGCGGCTT ATGGATTCCG GTTGCAGAGC 
CTTGATGCGC TTCTGGATAT GAAATCAACA GACCGGAAAC AGACACTACT TCATTATATA 
GTGCGGGTGA TCCAAGAAAA ATACCCTGAG TTCCTGAACT TTTCCAGTGA GCTGCATTTT 
CTGGACAAAG CAGGGGCAGT GTCACTGGAC AGTGTGCTTC AAGATGTGAG AAGCCTACAG 
CGGGGCATGG AGCTAACACG CAAAGAATTC ATGCGCCAGG ATGACAGTAT CATACTCAAG 
GATTTCCTAA AGACAAACAT AGAAATGATG GAAAAGCTTC AGGCAGACAG TAAAACTGCA 
CAGGAGGCAT ACGAGTCAAC AGTGGAATAC TTTGGGGAGA ATCCCAAGAC TACTCCTCCA 
ACTATGTTCT TCCCTACATT TGTACGCTTT ATTAAAGCAT ACCAGAAAGC AGAGGAAGAT 
ATAAAAGTGT GGAAGAAGCA AGAAGCAGCT GCCAAAGAAG AGGAGCAAGT TTCCCCCGGC 
AAAGGGGAAC AGGCAGTACC CAAGCAATCT CCCACCCATA AGGCCAAGCG ACAACAGATG 
GACCTCATAA CTGAGCTGAA GAAAAAGCAG ATGGTGAAAG AGCCTCTCAT TTATGAAGAA 
AAAGATGGTG CCATTGAAGA CATTATTTCA GGTCTACGTA TCCAGCCATA CCGGCGGGGG 
GACACTGGTC GGCACAGTGG CAAAAAACGA ACCACTGGGC AGATTCTCCA GGCAACCTCC 
GAGATCTCAC TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013913776.1
CDS	810..4064
Translation	MGNAASSLEMSPAKEIKTQPTASSPLPPKQPAPPKLPMPNAEELEERFGHVLNSMNLPPDKMKLLSQYDNEKKWELICDQERFQVKNPPSAYIQKLKSYLDTGGFSGKFKRRVQESTQILRELEISLRTNHIGWVQEFLNEENRGLDVLVDYLAFAQCSVTFNPAHSRKALRNSRIVSQKDDVHVCIMCLRAIMNYQSGFSLVMNHPACVNEITLSLNNKNPRTKALVLELLAAVCLVRGGHDIILAAFDNFKEVCGEKNRFEKLMEYFRNEDSNIDFMVACMQFINIVVHSVENMNFRAFLQYEFTQLGLDEYLQKLRCTESDKLQVQIQAYLDNIFDVGALLEDTETKNAILEHIEDLQEEVGQLTEKLQDAENESEAKIAELEKQLYQSRNELEILRARTSDEPEKLDEISKVNKPESPSWFPMQLPPESKITPQYSALEHNLEELEDRGFIRIQRGPGSDSIIGIEVLPVINIEASSSTSTDYPSPSTSPVRSLAPEIPPAPPLPSTKGSDSGAFYSSQEEGLPPIPTSLPPPPPPPLPGINEPPPAPPLPVIVTTSQASSTPPPPPPPPLLTFGGPVAPPPPPPPPMIDGATLPASTVTAAVKIKKPIQTKFRMPVFNWVALKPNQINGTIFNELNDEKVLQELDMNDFEEQFKTKAQGPGKEFSSIKTKTLQKAPTKVSLIECNRAKNLAITLRKGGLSIDSICKAIQTYDLQTLNLDFLELLMRFIPTEYEMTLIRKYEKEQRPLDELSDEDQFMIKFSKIPRLTERMNIMTFLGSFADTVQLLLPQLNFVIVASMSVKSSTKLRQILEIVLAFGNYLNSSKRGAAYGFRLQSLDALLDMKSTDRKQTLLHYIVRVIQEKYPEFLNFSSELHFLDKAGAVSLDSVLQDVRSLQRGMELTRKEFMRQDDSIILKDFLKTNIEMMEKLQADSKTAQEAYESTVEYFGENPKTTPPTMFFPTFVRFIKAYQKAEEDIKVWKKQEAAAKEEEQVSPGKGEQAVPKQSPTHKAKRQQMDLITELKKKQMVKEPLIYEEKDGAIEDIISGLRIQPYRRGDTGRHSGKKRTTGQILQATSEISL

Target ORF information:

RefSeq Version	XM_014058301.1
Organism	Thamnophis sirtalis
Definition	Thamnophis sirtalis formin-like 1 (FMNL1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014058301.1

ORF Insert Sequence:

ATGGGTAATG CAGCCAGCAG CCTGGAAATG TCTCCAGCGA AGGAGATTAA AACCCAACCG 
ACTGCTTCTT CGCCTTTACC CCCTAAACAG CCTGCACCCC CCAAACTACC GATGCCTAAT 
GCTGAGGAAT TGGAAGAACG ATTCGGACAT GTTCTGAATT CAATGAATCT GCCACCAGAC 
AAAATGAAGC TTCTGAGCCA GTATGATAAT GAAAAGAAAT GGGAATTAAT ATGTGACCAG 
GAACGATTCC AGGTCAAAAA CCCACCATCT GCCTATATCC AGAAACTGAA AAGCTATCTA 
GACACAGGTG GATTCAGTGG AAAGTTTAAG AGACGAGTTC AAGAATCTAC CCAGATTCTT 
CGGGAGTTAG AGATATCCCT GAGAACAAAC CACATTGGTT GGGTCCAGGA GTTCCTAAAT 
GAAGAGAATA GAGGTCTTGA TGTGTTGGTG GATTATCTTG CTTTTGCCCA GTGCTCTGTC 
ACATTCAATC CGGCACACAG CAGGAAGGCA TTACGGAACT CACGCATCGT CAGCCAGAAG 
GATGATGTTC ATGTGTGCAT CATGTGTCTC CGTGCCATTA TGAATTATCA GTCTGGATTC 
AGCCTTGTGA TGAACCACCC TGCCTGTGTA AATGAGATCA CACTCAGCCT CAACAATAAG 
AATCCCAGAA CTAAAGCTCT TGTCCTTGAG CTATTGGCTG CTGTTTGCTT GGTCCGAGGA 
GGACACGACA TTATTCTGGC TGCCTTTGAC AACTTCAAAG AGGTTTGTGG TGAGAAGAAC 
CGTTTTGAGA AGCTGATGGA GTATTTCCGA AATGAAGACA GCAACATAGA TTTCATGGTG 
GCTTGTATGC AGTTCATCAA TATTGTGGTT CATTCTGTGG AGAACATGAA CTTCCGTGCC 
TTCTTACAAT ATGAGTTTAC ACAGTTGGGA CTTGATGAGT ACCTACAGAA GCTGCGTTGT 
ACAGAAAGTG ATAAACTGCA AGTGCAGATC CAAGCCTATC TGGATAATAT CTTTGATGTT 
GGTGCCTTAC TGGAGGATAC CGAGACCAAA AATGCCATCC TGGAACACAT AGAGGATTTG 
CAAGAAGAGG TTGGACAGCT CACTGAGAAA CTCCAGGATG CAGAAAACGA ATCCGAAGCC 
AAAATTGCTG AGCTAGAGAA ACAGCTGTAT CAGTCACGCA ATGAGCTGGA GATCCTGAGA 
GCAAGAACTA GTGATGAGCC GGAGAAGCTA GATGAAATCA GCAAAGTTAA CAAGCCAGAA 
AGTCCTTCTT GGTTCCCAAT GCAGCTTCCA CCAGAGTCAA AAATAACTCC ACAATACTCT 
GCCTTGGAGC ACAATCTGGA AGAACTAGAA GACAGAGGTT TTATCCGGAT CCAGCGTGGG 
CCTGGCAGTG ATAGCATCAT TGGCATTGAG GTCCTCCCTG TTATTAATAT TGAAGCATCT 
TCGTCAACTA GCACAGATTA TCCCTCCCCA TCTACATCTC CTGTCCGCAG TCTTGCTCCA 
GAAATACCAC CAGCACCACC TCTACCTTCC ACGAAAGGCA GTGATTCTGG GGCTTTCTAT 
TCTTCTCAAG AGGAGGGGTT GCCACCTATT CCAACTTCTC TTCCACCACC ACCACCACCA 
CCCTTACCAG GGATCAATGA GCCTCCACCT GCCCCACCAC TGCCTGTGAT TGTAACTACA 
TCTCAAGCTT CATCAACGCC TCCTCCTCCT CCGCCACCAC CCCTCTTAAC ATTTGGTGGC 
CCAGTTGCTC CTCCTCCTCC ACCACCGCCT CCCATGATTG ATGGAGCAAC TTTGCCAGCC 
AGTACAGTTA CTGCAGCTGT CAAGATCAAG AAGCCTATAC AGACCAAATT CCGTATGCCT 
GTCTTCAATT GGGTTGCTTT GAAGCCTAAC CAGATCAATG GGACCATCTT CAATGAACTC 
AATGATGAAA AAGTCCTTCA GGAGCTGGAC ATGAATGACT TTGAAGAACA ATTCAAAACC 
AAAGCTCAGG GTCCAGGCAA AGAATTCAGT TCCATAAAGA CCAAGACTTT ACAGAAAGCT 
CCCACTAAGG TTTCACTGAT TGAATGCAAC CGGGCCAAGA ACCTGGCCAT CACCCTCCGT 
AAAGGGGGGC TCAGTATCGA CAGCATTTGC AAAGCCATTC AGACATACGA CTTACAAACC 
CTCAATCTGG ATTTTCTGGA GTTACTGATG CGCTTCATCC CAACGGAATA TGAGATGACA 
CTAATCCGCA AATATGAAAA AGAGCAACGT CCACTGGATG AGCTTTCAGA TGAGGACCAA 
TTCATGATCA AATTCAGCAA AATTCCCCGC CTTACAGAAC GCATGAACAT CATGACATTC 
TTAGGCAGTT TTGCAGATAC CGTACAACTC CTTCTGCCAC AACTAAATTT TGTGATTGTT 
GCTTCAATGT CAGTCAAGTC ATCTACCAAA CTGCGTCAGA TTTTAGAGAT TGTCCTGGCA 
TTTGGGAATT ATTTGAACAG CAGCAAACGG GGAGCGGCTT ATGGATTCCG GTTGCAGAGC 
CTTGATGCGC TTCTGGATAT GAAATCAACA GACCGGAAAC AGACACTACT TCATTATATA 
GTGCGGGTGA TCCAAGAAAA ATACCCTGAG TTCCTGAACT TTTCCAGTGA GCTGCATTTT 
CTGGACAAAG CAGGGGCAGT GTCACTGGAC AGTGTGCTTC AAGATGTGAG AAGCCTACAG 
CGGGGCATGG AGCTAACACG CAAAGAATTC ATGCGCCAGG ATGACAGTAT CATACTCAAG 
GATTTCCTAA AGACAAACAT AGAAATGATG GAAAAGCTTC AGGCAGACAG TAAAACTGCA 
CAGGAGGCAT ACGAGTCAAC AGTGGAATAC TTTGGGGAGA ATCCCAAGAC TACTCCTCCA 
ACTATGTTCT TCCCTACATT TGTACGCTTT ATTAAAGCAT ACCAGAAAGC AGAGGAAGAT 
ATAAAAGTGT GGAAGAAGCA AGAAGCAGCT GCCAAAGAAG AGGAGCAAGT TTCCCCCGGC 
AAAGGGGAAC AGGCAGTACC CAAGCAATCT CCCACCCATA AGGCCAAGCG ACAACAGATG 
GACCTCATAA CTGAGCTGAA GAAAAAGCAG ATGGTGAAAG AGCCTCTCAT TTATGAAGAA 
AAAGATGGTG CCATTGAAGA CATTATTTCA GGTCTACGTA TCCAGCCATA CCGGCGGGGG 
GACACTGGTC GGCACAGTGG CAAAAAACGA ACCACTGGGC AGATTCTCCA GGCAACCTCC 
GAGATCTCAC TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FMNL1 ( XM_014058303.1 ) cDNA ORF clone, Thamnophis sirtalis -> XP_013913778.1 Thamnophis sirtalis formin-like 1 (FMNL1), transcript variant X8, mRNA.

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CloneID	OTh18457
Clone ID Related Accession (Same CDS sequence)	XM_014058303.1
Accession Version	XM_014058303.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3381bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-10
Organism	Thamnophis sirtalis
Product	formin-like protein 1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_013658128.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Thamnophis sirtalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTAATG CAGCCAGCAG CCTGGAAATG TCTCCAGCGA AGGAGATTAA AACCCAACCG 
ACTGCTTCTT CGCCTTTACC CCCTAAACAG CCTGCACCCC CCAAACTACC GATGCCTAAT 
GCTGAGGAAT TGGAAGAACG ATTCGGACAT GTTCTGAATT CAATGAATCT GCCACCAGAC 
AAAATGAAGC TTCTGAGCCA GTATGATAAT GAAAAGAAAT GGGAATTAAT ATGTGACCAG 
GAACGATTCC AGGTCAAAAA CCCACCATCT GCCTATATCC AGAAACTGAA AAGCTATCTA 
GACACAGGTG GATTCAGTGG AAAGTTTAAG AGACGAGTTC AAGAATCTAC CCAGATTCTT 
CGGGAGTTAG AGATATCCCT GAGAACAAAC CACATTGGTT GGGTCCAGGA GTTCCTAAAT 
GAAGAGAATA GAGGTCTTGA TGTGTTGGTG GATTATCTTG CTTTTGCCCA GTGCTCTGTC 
ACGTATGATA TGGACAGCAC TGACAATGGG AGCCCTGGGT CGGACAAGGG AAAGGCCCTG 
GACAGATCTG TAGAGGACCT CAGCAGAAGC AATTGCTCTC CCACACAGGG TTCCATCAAA 
GCGAGGCACT TGACTGTCAG ATTCAATCCG GCACACAGCA GGAAGGCATT ACGGAACTCA 
CGCATCGTCA GCCAGAAGGA TGATGTTCAT GTGTGCATCA TGTGTCTCCG TGCCATTATG 
AATTATCAGT CTGGATTCAG CCTTGTGATG AACCACCCTG CCTGTGTAAA TGAGATCACA 
CTCAGCCTCA ACAATAAGAA TCCCAGAACT AAAGCTCTTG TCCTTGAGCT ATTGGCTGCT 
GTTTGCTTGG TCCGAGGAGG ACACGACATT ATTCTGGCTG CCTTTGACAA CTTCAAAGAG 
GTTTGTGGTG AGAAGAACCG TTTTGAGAAG CTGATGGAGT ATTTCCGAAA TGAAGACAGC 
AACATAGATT TCATGGTGGC TTGTATGCAG TTCATCAATA TTGTGGTTCA TTCTGTGGAG 
AACATGAACT TCCGTGCCTT CTTACAATAT GAGTTTACAC AGTTGGGACT TGATGAGTAC 
CTACAGAAGC TGCGTTGTAC AGAAAGTGAT AAACTGCAAG TGCAGATCCA AGCCTATCTG 
GATAATATCT TTGATGTTGG TGCCTTACTG GAGGATACCG AGACCAAAAA TGCCATCCTG 
GAACACATAG AGGATTTGCA AGAAGAGGTT GGACAGCTCA CTGAGAAACT CCAGGATGCA 
GAAAACGAAT CCGAAGCCAA AATTGCTGAG CTAGAGAAAC AGCTGTATCA GTCACGCAAT 
GAGCTGGAGA TCCTGAGAGC AAGAACTAGT GATGAGCCGG AGAAGCTAGA TGAAATCAGC 
AAAGTTAACA AGCCAGAAAG TCCTTCTTGG TTCCCAATGC AGCTTCCACC AGAGTCAAAA 
ATAACTCCAC AATACTCTGC CTTGGAGCAC AATCTGGAAG AACTAGAAGA CAGAGGTTTT 
ATCCGGATCC AGCGTGGGCC TGGCAGTGAT AGCATCATTG GCATTGAGGT CCTCCCTGTT 
ATTAATATTG AAGCATCTTC GTCAACTAGC ACAGATTATC CCTCCCCATC TACATCTCCT 
GTCCGCAGTC TTGCTCCAGA AATACCACCA GCACCACCTC TACCTTCCAC GAAAGGCAGT 
GATTCTGGGG CTTTCTATTC TTCTCAAGAG GAGGGGTTGC CACCTATTCC AACTTCTCTT 
CCACCACCAC CACCACCACC CTTACCAGGG ATCAATGAGC CTCCACCTGC CCCACCACTG 
CCTGTGATTG TAACTACATC TCAAGCTTCA TCAACGCCTC CTCCTCCTCC GCCACCACCC 
CTCTTAACAT TTGGTGGCCC AGTTGCTCCT CCTCCTCCAC CACCGCCTCC CATGATTGAT 
GGAGCAACTT TGCCAGCCAG TACAGTTACT GCAGCTGTCA AGATCAAGAA GCCTATACAG 
ACCAAATTCC GTATGCCTGT CTTCAATTGG GTTGCTTTGA AGCCTAACCA GATCAATGGG 
ACCATCTTCA ATGAACTCAA TGATGAAAAA GTCCTTCAGG AGCTGGACAT GAATGACTTT 
GAAGAACAAT TCAAAACCAA AGCTCAGGGT CCAGGCAAAG AATTCAGTTC CATAAAGACC 
AAGACTTTAC AGAAAGCTCC CACTAAGGTT TCACTGATTG AATGCAACCG GGCCAAGAAC 
CTGGCCATCA CCCTCCGTAA AGGGGGGCTC AGTATCGACA GCATTTGCAA AGCCATTCAG 
ACATACGACT TACAAACCCT CAATCTGGAT TTTCTGGAGT TACTGATGCG CTTCATCCCA 
ACGGAATATG AGATGACACT AATCCGCAAA TATGAAAAAG AGCAACGTCC ACTGGATGAG 
CTTTCAGATG AGGACCAATT CATGATCAAA TTCAGCAAAA TTCCCCGCCT TACAGAACGC 
ATGAACATCA TGACATTCTT AGGCAGTTTT GCAGATACCG TACAACTCCT TCTGCCACAA 
CTAAATTTTG TGATTGTTGC TTCAATGTCA GTCAAGTCAT CTACCAAACT GCGTCAGATT 
TTAGAGATTG TCCTGGCATT TGGGAATTAT TTGAACAGCA GCAAACGGGG AGCGGCTTAT 
GGATTCCGGT TGCAGAGCCT TGATGCGCTT CTGGATATGA AATCAACAGA CCGGAAACAG 
ACACTACTTC ATTATATAGT GCGGGTGATC CAAGAAAAAT ACCCTGAGTT CCTGAACTTT 
TCCAGTGAGC TGCATTTTCT GGACAAAGCA GGGGCAGTGT CACTGGACAG TGTGCTTCAA 
GATGTGAGAA GCCTACAGCG GGGCATGGAG CTAACACGCA AAGAATTCAT GCGCCAGGAT 
GACAGTATCA TACTCAAGGA TTTCCTAAAG ACAAACATAG AAATGATGGA AAAGCTTCAG 
GCAGACAGTA AAACTGCACA GGAGGCATAC GAGTCAACAG TGGAATACTT TGGGGAGAAT 
CCCAAGACTA CTCCTCCAAC TATGTTCTTC CCTACATTTG TACGCTTTAT TAAAGCATAC 
CAGAAAGCAG AGGAAGATAT AAAAGTGTGG AAGAAGCAAG AAGCAGCTGC CAAAGAAGAG 
GAGCAAGTTT CCCCCGGCAA AGGGGAACAG GCAGTACCCA AGCAATCTCC CACCCATAAG 
GCCAAGCGAC AACAGATGGA CCTCATAACT GAGCTGAAGA AAAAGCAGAT GGTGAAAGAG 
CCTCTCATTT ATGAAGAAAA AGATGGTGCC ATTGAAGACA TTATTTCAGC TTTGAAAACT 
ATTCCTTTTA CTGCTCGGAC GGGAAAACGC TCATCTCGCC TCTTCTGCGA TGCCAGCTTC 
AATGAAGAAA GCCCACTGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013913778.1
CDS	809..4189
Translation	MGNAASSLEMSPAKEIKTQPTASSPLPPKQPAPPKLPMPNAEELEERFGHVLNSMNLPPDKMKLLSQYDNEKKWELICDQERFQVKNPPSAYIQKLKSYLDTGGFSGKFKRRVQESTQILRELEISLRTNHIGWVQEFLNEENRGLDVLVDYLAFAQCSVTYDMDSTDNGSPGSDKGKALDRSVEDLSRSNCSPTQGSIKARHLTVRFNPAHSRKALRNSRIVSQKDDVHVCIMCLRAIMNYQSGFSLVMNHPACVNEITLSLNNKNPRTKALVLELLAAVCLVRGGHDIILAAFDNFKEVCGEKNRFEKLMEYFRNEDSNIDFMVACMQFINIVVHSVENMNFRAFLQYEFTQLGLDEYLQKLRCTESDKLQVQIQAYLDNIFDVGALLEDTETKNAILEHIEDLQEEVGQLTEKLQDAENESEAKIAELEKQLYQSRNELEILRARTSDEPEKLDEISKVNKPESPSWFPMQLPPESKITPQYSALEHNLEELEDRGFIRIQRGPGSDSIIGIEVLPVINIEASSSTSTDYPSPSTSPVRSLAPEIPPAPPLPSTKGSDSGAFYSSQEEGLPPIPTSLPPPPPPPLPGINEPPPAPPLPVIVTTSQASSTPPPPPPPPLLTFGGPVAPPPPPPPPMIDGATLPASTVTAAVKIKKPIQTKFRMPVFNWVALKPNQINGTIFNELNDEKVLQELDMNDFEEQFKTKAQGPGKEFSSIKTKTLQKAPTKVSLIECNRAKNLAITLRKGGLSIDSICKAIQTYDLQTLNLDFLELLMRFIPTEYEMTLIRKYEKEQRPLDELSDEDQFMIKFSKIPRLTERMNIMTFLGSFADTVQLLLPQLNFVIVASMSVKSSTKLRQILEIVLAFGNYLNSSKRGAAYGFRLQSLDALLDMKSTDRKQTLLHYIVRVIQEKYPEFLNFSSELHFLDKAGAVSLDSVLQDVRSLQRGMELTRKEFMRQDDSIILKDFLKTNIEMMEKLQADSKTAQEAYESTVEYFGENPKTTPPTMFFPTFVRFIKAYQKAEEDIKVWKKQEAAAKEEEQVSPGKGEQAVPKQSPTHKAKRQQMDLITELKKKQMVKEPLIYEEKDGAIEDIISALKTIPFTARTGKRSSRLFCDASFNEESPL

Target ORF information:

RefSeq Version	XM_014058303.1
Organism	Thamnophis sirtalis
Definition	Thamnophis sirtalis formin-like 1 (FMNL1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014058303.1

ORF Insert Sequence:

ATGGGTAATG CAGCCAGCAG CCTGGAAATG TCTCCAGCGA AGGAGATTAA AACCCAACCG 
ACTGCTTCTT CGCCTTTACC CCCTAAACAG CCTGCACCCC CCAAACTACC GATGCCTAAT 
GCTGAGGAAT TGGAAGAACG ATTCGGACAT GTTCTGAATT CAATGAATCT GCCACCAGAC 
AAAATGAAGC TTCTGAGCCA GTATGATAAT GAAAAGAAAT GGGAATTAAT ATGTGACCAG 
GAACGATTCC AGGTCAAAAA CCCACCATCT GCCTATATCC AGAAACTGAA AAGCTATCTA 
GACACAGGTG GATTCAGTGG AAAGTTTAAG AGACGAGTTC AAGAATCTAC CCAGATTCTT 
CGGGAGTTAG AGATATCCCT GAGAACAAAC CACATTGGTT GGGTCCAGGA GTTCCTAAAT 
GAAGAGAATA GAGGTCTTGA TGTGTTGGTG GATTATCTTG CTTTTGCCCA GTGCTCTGTC 
ACGTATGATA TGGACAGCAC TGACAATGGG AGCCCTGGGT CGGACAAGGG AAAGGCCCTG 
GACAGATCTG TAGAGGACCT CAGCAGAAGC AATTGCTCTC CCACACAGGG TTCCATCAAA 
GCGAGGCACT TGACTGTCAG ATTCAATCCG GCACACAGCA GGAAGGCATT ACGGAACTCA 
CGCATCGTCA GCCAGAAGGA TGATGTTCAT GTGTGCATCA TGTGTCTCCG TGCCATTATG 
AATTATCAGT CTGGATTCAG CCTTGTGATG AACCACCCTG CCTGTGTAAA TGAGATCACA 
CTCAGCCTCA ACAATAAGAA TCCCAGAACT AAAGCTCTTG TCCTTGAGCT ATTGGCTGCT 
GTTTGCTTGG TCCGAGGAGG ACACGACATT ATTCTGGCTG CCTTTGACAA CTTCAAAGAG 
GTTTGTGGTG AGAAGAACCG TTTTGAGAAG CTGATGGAGT ATTTCCGAAA TGAAGACAGC 
AACATAGATT TCATGGTGGC TTGTATGCAG TTCATCAATA TTGTGGTTCA TTCTGTGGAG 
AACATGAACT TCCGTGCCTT CTTACAATAT GAGTTTACAC AGTTGGGACT TGATGAGTAC 
CTACAGAAGC TGCGTTGTAC AGAAAGTGAT AAACTGCAAG TGCAGATCCA AGCCTATCTG 
GATAATATCT TTGATGTTGG TGCCTTACTG GAGGATACCG AGACCAAAAA TGCCATCCTG 
GAACACATAG AGGATTTGCA AGAAGAGGTT GGACAGCTCA CTGAGAAACT CCAGGATGCA 
GAAAACGAAT CCGAAGCCAA AATTGCTGAG CTAGAGAAAC AGCTGTATCA GTCACGCAAT 
GAGCTGGAGA TCCTGAGAGC AAGAACTAGT GATGAGCCGG AGAAGCTAGA TGAAATCAGC 
AAAGTTAACA AGCCAGAAAG TCCTTCTTGG TTCCCAATGC AGCTTCCACC AGAGTCAAAA 
ATAACTCCAC AATACTCTGC CTTGGAGCAC AATCTGGAAG AACTAGAAGA CAGAGGTTTT 
ATCCGGATCC AGCGTGGGCC TGGCAGTGAT AGCATCATTG GCATTGAGGT CCTCCCTGTT 
ATTAATATTG AAGCATCTTC GTCAACTAGC ACAGATTATC CCTCCCCATC TACATCTCCT 
GTCCGCAGTC TTGCTCCAGA AATACCACCA GCACCACCTC TACCTTCCAC GAAAGGCAGT 
GATTCTGGGG CTTTCTATTC TTCTCAAGAG GAGGGGTTGC CACCTATTCC AACTTCTCTT 
CCACCACCAC CACCACCACC CTTACCAGGG ATCAATGAGC CTCCACCTGC CCCACCACTG 
CCTGTGATTG TAACTACATC TCAAGCTTCA TCAACGCCTC CTCCTCCTCC GCCACCACCC 
CTCTTAACAT TTGGTGGCCC AGTTGCTCCT CCTCCTCCAC CACCGCCTCC CATGATTGAT 
GGAGCAACTT TGCCAGCCAG TACAGTTACT GCAGCTGTCA AGATCAAGAA GCCTATACAG 
ACCAAATTCC GTATGCCTGT CTTCAATTGG GTTGCTTTGA AGCCTAACCA GATCAATGGG 
ACCATCTTCA ATGAACTCAA TGATGAAAAA GTCCTTCAGG AGCTGGACAT GAATGACTTT 
GAAGAACAAT TCAAAACCAA AGCTCAGGGT CCAGGCAAAG AATTCAGTTC CATAAAGACC 
AAGACTTTAC AGAAAGCTCC CACTAAGGTT TCACTGATTG AATGCAACCG GGCCAAGAAC 
CTGGCCATCA CCCTCCGTAA AGGGGGGCTC AGTATCGACA GCATTTGCAA AGCCATTCAG 
ACATACGACT TACAAACCCT CAATCTGGAT TTTCTGGAGT TACTGATGCG CTTCATCCCA 
ACGGAATATG AGATGACACT AATCCGCAAA TATGAAAAAG AGCAACGTCC ACTGGATGAG 
CTTTCAGATG AGGACCAATT CATGATCAAA TTCAGCAAAA TTCCCCGCCT TACAGAACGC 
ATGAACATCA TGACATTCTT AGGCAGTTTT GCAGATACCG TACAACTCCT TCTGCCACAA 
CTAAATTTTG TGATTGTTGC TTCAATGTCA GTCAAGTCAT CTACCAAACT GCGTCAGATT 
TTAGAGATTG TCCTGGCATT TGGGAATTAT TTGAACAGCA GCAAACGGGG AGCGGCTTAT 
GGATTCCGGT TGCAGAGCCT TGATGCGCTT CTGGATATGA AATCAACAGA CCGGAAACAG 
ACACTACTTC ATTATATAGT GCGGGTGATC CAAGAAAAAT ACCCTGAGTT CCTGAACTTT 
TCCAGTGAGC TGCATTTTCT GGACAAAGCA GGGGCAGTGT CACTGGACAG TGTGCTTCAA 
GATGTGAGAA GCCTACAGCG GGGCATGGAG CTAACACGCA AAGAATTCAT GCGCCAGGAT 
GACAGTATCA TACTCAAGGA TTTCCTAAAG ACAAACATAG AAATGATGGA AAAGCTTCAG 
GCAGACAGTA AAACTGCACA GGAGGCATAC GAGTCAACAG TGGAATACTT TGGGGAGAAT 
CCCAAGACTA CTCCTCCAAC TATGTTCTTC CCTACATTTG TACGCTTTAT TAAAGCATAC 
CAGAAAGCAG AGGAAGATAT AAAAGTGTGG AAGAAGCAAG AAGCAGCTGC CAAAGAAGAG 
GAGCAAGTTT CCCCCGGCAA AGGGGAACAG GCAGTACCCA AGCAATCTCC CACCCATAAG 
GCCAAGCGAC AACAGATGGA CCTCATAACT GAGCTGAAGA AAAAGCAGAT GGTGAAAGAG 
CCTCTCATTT ATGAAGAAAA AGATGGTGCC ATTGAAGACA TTATTTCAGC TTTGAAAACT 
ATTCCTTTTA CTGCTCGGAC GGGAAAACGC TCATCTCGCC TCTTCTGCGA TGCCAGCTTC 
AATGAAGAAA GCCCACTGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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03-6811-6572

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FMNL1 cDNA ORF clone, Thamnophis sirtalis

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム