LOC107359221 cDNA ORF clone, Tetranychus urticae(two-spotted spider mite)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC107359221
Entrez Gene ID	107359221
Full Name	putative pre-mRNA-splicing factor ATP-dependent RNA helicase DHX16
Gene Type	protein-coding
Organism	Tetranychus urticae(two-spotted spider mite)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015925693.1	XP_015781179.1	putative pre-mRNA-splicing factor ATP-dependent RNA helicase DHX16
XM_015925693.2	XP_015781179.1	pre-mRNA-splicing factor ATP-dependent RNA helicase DHX16-like

Pathways from BioSystems
Gene Ontology

The following LOC107359221 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC107359221 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OTe15710	XM_015925693.2 Latest version!	Tetranychus urticae pre-mRNA-splicing factor ATP-dependent RNA helicase DHX16-like (LOC107359221), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
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OTe15710	XM_015925693.1	Tetranychus urticae putative pre-mRNA-splicing factor ATP-dependent RNA helicase DHX16 (LOC107359221), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC107359221 ( XM_015925693.1 ) cDNA ORF clone, Tetranychus urticae(two-spotted spider mite) -> XP_015781179.1 Tetranychus urticae putative pre-mRNA-splicing factor ATP-dependent RNA helicase DHX16 (LOC107359221), mRNA.

Price & Availability↑

CloneID	OTe15710
Clone ID Related Accession (Same CDS sequence)	XM_015925693.1 , XM_015925693.2
Accession Version	XM_015925693.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2652bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-03-15
Organism	Tetranychus urticae(two-spotted spider mite)
Product	putative pre-mRNA-splicing factor ATP-dependent RNA helicase DHX16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015449932.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Tetranychus urticae Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCATCGA GTAAACAAAC TTCATCAAAT CATGATGAAG AAGAAAGACT CAAAGATATT 
GCAGAAAGAG ATGCTTATGC AGAGAGACTC AGAGAAAAGG ACCAAAAAAG GACTCGTAAT 
GTGGCCACCA AATCAGATGA AAGGATGTAC GCTGAAGCAG CGAAAAGACT AAAACTGGAA 
AATGAGGATA GAAAAGCTAT AGTACCAAAA CTGCGAGAGG AATCGAGAAA AAACTATCTA 
ATCAAACGAA AGGCCGATCA ATTGGTTTTG TTAGAAGGGG AAATTAAGGA TGAAGAACAG 
ATTTTCAGTG ATGTACCCTT AACTAAAGCT GAAAAGGCTG AATATGAATA CAAGAAAAAG 
GTTCTTACTT TGGCTAAGGA ACATGAACAG GCTAAAGAAT TGGAAAGTAT TAGAAGATAT 
CACATTCCAG AAGAAAGTAA ACCAAGCAAA GTTTATGAAG AAATCGATGA AAGGGAGAAA 
CAACCAAACG CTGAAGCTAG AAAATGGGAA GAAGAAAGGT TAAATACTGC CAAATTATCA 
TTCGGAGCAA AAAATAAATC CAAAAAGGTT GAAGAATATG ATATTTTAAT TGATGCTTCA 
ATCGACTTCA TCAAAGCACT AACGGTTGGT GGACAAAATA CTGACGAAAA AGATATAGTT 
GACGAAGAAA CAGCAAAAAG AATGACAATT GCTGAAACAA GAAGATCATT ACCGATTTAT 
GCTTACAAAA GAGAACTCAT CAAAGCAGTA AAAGAGCATC AAATTTTAAT CATTGAAGGA 
GAGACAGGAT CTGGAAAGAC AACACAAATT CCCCAATATT TACATGAAGC AGGTTTCACC 
AAAGGAGGTA AAAAAGTTGG TTGCACACAG CCAAGAAGAG TAGCTGCTAT GAGCGTAGCG 
GCCCGTGTTG CTCAAGAAAT GAATGTTAAA CTTGGCCATG AAGTCGGTTA TAGTATTCGT 
TTTGAAGATT GTACTAGTGA GCGAACTGTG ATTAAATACA TGACTGATGG TATGTTACTC 
CGAGAATTCC TCTCTGAACC TGATTTAGCA TCTTATTCAT GCTTGATTGT TGACGAAGCA 
CACGAGAGAA CCCTTCATAC AGACATTTTG TTTGGCTTAG TTAAAGATAT AGCTCGATTT 
AGACCTGACT TTAAGCTTCT CATCTCCAGT GCCACTCTGG ATGCCGAAAA ATTCAGCGTC 
TTTTTTGATG AAGCTCCAGT TTTTCGTATA CCAGGTCGTC GGTTTCCAGT TGATATTTAT 
TACACCTCAG CACCTGAAGC TGATTACATA GATGCCTGTG TAGTTACTAT TTTACAAATA 
CACATCACAC AACCTTTAGG TGATATTCTC GTCTTTCTTC CCGGTCAAGA GGAAATCGAA 
CAATGTTCTG AAGTTTTGAT GGAACGACAA CGAAAGTTAG GATCAAAAAT CAGGGAACTT 
ATAATCAGAC CAATTTACGC TAACTTGCCT CAAGAATTAC AAGCTCAAGT TTTCGAACCA 
ACTCCACCGG GTGCTCGGAA AGTTGTAATT GCTACCAACA TTGCTGAAAC TTCATTAACA 
ATAGATGGTA TTATTTATGT CATCGATCCT GGTTATTGTA AATTAAATAT TTATAATCCG 
AGATCTCGTA TGGAATCTTT AGTTGTCCAA CCAGTCTCTA AAGCTAGTGC TAATCAAAGA 
GCCGGTAGAG CTGGTCGTGT TGCTGCCGGT AAATGTTTTA GACTGTTCAC TGCATGGGCA 
TATCAGAATG AGCTTGAGGA TAATACTGTT CCTGAAATTC AAAGGGTTCA TCTTGGTAAC 
GCCGTTTTAC TTTTGAAAAG TTTAGGAATC AATGACATTA TTCATTTTGA CTTTTTGGAT 
CCACCGCCAT CTGAAACGCT TGTACTTGCC TTAGAACAAT TATATGCATT GGGAGCTCTT 
AATCAATTTG GTGAACTTAC TCGACTTGGT CGTAAAATGG CTGAATTTCC TATTGACCCA 
ATGATGTCCA AAGCTTTAAT TGCATCCGAA AAATATAACT GTAGTGTTGA GATAATGACC 
ATTTGCGCCA TGCTCAGTGT TAATTCCAAT ATTTTTTATC GGCCAAAAGA CAAAGCTATT 
CATGCAGATA CGGCAAGGAA AAACTTTTTT CAACCTGGAG GGGATCATCT GATGCTGTTG 
AATGTTTATA ATCAATGGGT TGAAATGGGA TATACTAAAC AATGGTGTGC TGAAAATTTT 
ATTCAATATC GAGCGCTAGT TAGAGCTCGA GATATACGAG ATCAACTAGA AAGTCTAATG 
GATCGAGCCG AAGTAGAAAT TAAATCAAAT CCAACTGAAT TTGTACTCAT CCGTAAAGCC 
ATCTTAACAG GTTATTTTTA TCATGTTGCC CGTTTAGGAG GTGGTGGAGG CTCATATCGT 
ACTTTAAAGC AACATCAACC CGTTTCAATT CATCCTAATT CATCATTGTT TGATGAAGAA 
CCTCGATGGG TTCTCTATCA TGAACTAGTT CTTACAACCA AAGAGTACAT GAGACAGGTT 
ATTGAAATTG AACCTAAATG GCTTGTAGAA ATTGCTCCAC ATTTTTATAA ATCCGAAGAG 
GTTGAAGATT TATGTAAGAA AATGCCGAAA AAAGTAGGAA AAGCAAAGGA AGAACTGGAA 
CGAAATTATT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015781179.1
CDS	200..2851
Translation	MSSSKQTSSNHDEEERLKDIAERDAYAERLREKDQKRTRNVATKSDERMYAEAAKRLKLENEDRKAIVPKLREESRKNYLIKRKADQLVLLEGEIKDEEQIFSDVPLTKAEKAEYEYKKKVLTLAKEHEQAKELESIRRYHIPEESKPSKVYEEIDEREKQPNAEARKWEEERLNTAKLSFGAKNKSKKVEEYDILIDASIDFIKALTVGGQNTDEKDIVDEETAKRMTIAETRRSLPIYAYKRELIKAVKEHQILIIEGETGSGKTTQIPQYLHEAGFTKGGKKVGCTQPRRVAAMSVAARVAQEMNVKLGHEVGYSIRFEDCTSERTVIKYMTDGMLLREFLSEPDLASYSCLIVDEAHERTLHTDILFGLVKDIARFRPDFKLLISSATLDAEKFSVFFDEAPVFRIPGRRFPVDIYYTSAPEADYIDACVVTILQIHITQPLGDILVFLPGQEEIEQCSEVLMERQRKLGSKIRELIIRPIYANLPQELQAQVFEPTPPGARKVVIATNIAETSLTIDGIIYVIDPGYCKLNIYNPRSRMESLVVQPVSKASANQRAGRAGRVAAGKCFRLFTAWAYQNELEDNTVPEIQRVHLGNAVLLLKSLGINDIIHFDFLDPPPSETLVLALEQLYALGALNQFGELTRLGRKMAEFPIDPMMSKALIASEKYNCSVEIMTICAMLSVNSNIFYRPKDKAIHADTARKNFFQPGGDHLMLLNVYNQWVEMGYTKQWCAENFIQYRALVRARDIRDQLESLMDRAEVEIKSNPTEFVLIRKAILTGYFYHVARLGGGGGSYRTLKQHQPVSIHPNSSLFDEEPRWVLYHELVLTTKEYMRQVIEIEPKWLVEIAPHFYKSEEVEDLCKKMPKKVGKAKEELERNY

Target ORF information:

RefSeq Version	XM_015925693.1
Organism	Tetranychus urticae(two-spotted spider mite)
Definition	Tetranychus urticae putative pre-mRNA-splicing factor ATP-dependent RNA helicase DHX16 (LOC107359221), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015925693.1

ORF Insert Sequence:

ATGTCATCGA GTAAACAAAC TTCATCAAAT CATGATGAAG AAGAAAGACT CAAAGATATT 
GCAGAAAGAG ATGCTTATGC AGAGAGACTC AGAGAAAAGG ACCAAAAAAG GACTCGTAAT 
GTGGCCACCA AATCAGATGA AAGGATGTAC GCTGAAGCAG CGAAAAGACT AAAACTGGAA 
AATGAGGATA GAAAAGCTAT AGTACCAAAA CTGCGAGAGG AATCGAGAAA AAACTATCTA 
ATCAAACGAA AGGCCGATCA ATTGGTTTTG TTAGAAGGGG AAATTAAGGA TGAAGAACAG 
ATTTTCAGTG ATGTACCCTT AACTAAAGCT GAAAAGGCTG AATATGAATA CAAGAAAAAG 
GTTCTTACTT TGGCTAAGGA ACATGAACAG GCTAAAGAAT TGGAAAGTAT TAGAAGATAT 
CACATTCCAG AAGAAAGTAA ACCAAGCAAA GTTTATGAAG AAATCGATGA AAGGGAGAAA 
CAACCAAACG CTGAAGCTAG AAAATGGGAA GAAGAAAGGT TAAATACTGC CAAATTATCA 
TTCGGAGCAA AAAATAAATC CAAAAAGGTT GAAGAATATG ATATTTTAAT TGATGCTTCA 
ATCGACTTCA TCAAAGCACT AACGGTTGGT GGACAAAATA CTGACGAAAA AGATATAGTT 
GACGAAGAAA CAGCAAAAAG AATGACAATT GCTGAAACAA GAAGATCATT ACCGATTTAT 
GCTTACAAAA GAGAACTCAT CAAAGCAGTA AAAGAGCATC AAATTTTAAT CATTGAAGGA 
GAGACAGGAT CTGGAAAGAC AACACAAATT CCCCAATATT TACATGAAGC AGGTTTCACC 
AAAGGAGGTA AAAAAGTTGG TTGCACACAG CCAAGAAGAG TAGCTGCTAT GAGCGTAGCG 
GCCCGTGTTG CTCAAGAAAT GAATGTTAAA CTTGGCCATG AAGTCGGTTA TAGTATTCGT 
TTTGAAGATT GTACTAGTGA GCGAACTGTG ATTAAATACA TGACTGATGG TATGTTACTC 
CGAGAATTCC TCTCTGAACC TGATTTAGCA TCTTATTCAT GCTTGATTGT TGACGAAGCA 
CACGAGAGAA CCCTTCATAC AGACATTTTG TTTGGCTTAG TTAAAGATAT AGCTCGATTT 
AGACCTGACT TTAAGCTTCT CATCTCCAGT GCCACTCTGG ATGCCGAAAA ATTCAGCGTC 
TTTTTTGATG AAGCTCCAGT TTTTCGTATA CCAGGTCGTC GGTTTCCAGT TGATATTTAT 
TACACCTCAG CACCTGAAGC TGATTACATA GATGCCTGTG TAGTTACTAT TTTACAAATA 
CACATCACAC AACCTTTAGG TGATATTCTC GTCTTTCTTC CCGGTCAAGA GGAAATCGAA 
CAATGTTCTG AAGTTTTGAT GGAACGACAA CGAAAGTTAG GATCAAAAAT CAGGGAACTT 
ATAATCAGAC CAATTTACGC TAACTTGCCT CAAGAATTAC AAGCTCAAGT TTTCGAACCA 
ACTCCACCGG GTGCTCGGAA AGTTGTAATT GCTACCAACA TTGCTGAAAC TTCATTAACA 
ATAGATGGTA TTATTTATGT CATCGATCCT GGTTATTGTA AATTAAATAT TTATAATCCG 
AGATCTCGTA TGGAATCTTT AGTTGTCCAA CCAGTCTCTA AAGCTAGTGC TAATCAAAGA 
GCCGGTAGAG CTGGTCGTGT TGCTGCCGGT AAATGTTTTA GACTGTTCAC TGCATGGGCA 
TATCAGAATG AGCTTGAGGA TAATACTGTT CCTGAAATTC AAAGGGTTCA TCTTGGTAAC 
GCCGTTTTAC TTTTGAAAAG TTTAGGAATC AATGACATTA TTCATTTTGA CTTTTTGGAT 
CCACCGCCAT CTGAAACGCT TGTACTTGCC TTAGAACAAT TATATGCATT GGGAGCTCTT 
AATCAATTTG GTGAACTTAC TCGACTTGGT CGTAAAATGG CTGAATTTCC TATTGACCCA 
ATGATGTCCA AAGCTTTAAT TGCATCCGAA AAATATAACT GTAGTGTTGA GATAATGACC 
ATTTGCGCCA TGCTCAGTGT TAATTCCAAT ATTTTTTATC GGCCAAAAGA CAAAGCTATT 
CATGCAGATA CGGCAAGGAA AAACTTTTTT CAACCTGGAG GGGATCATCT GATGCTGTTG 
AATGTTTATA ATCAATGGGT TGAAATGGGA TATACTAAAC AATGGTGTGC TGAAAATTTT 
ATTCAATATC GAGCGCTAGT TAGAGCTCGA GATATACGAG ATCAACTAGA AAGTCTAATG 
GATCGAGCCG AAGTAGAAAT TAAATCAAAT CCAACTGAAT TTGTACTCAT CCGTAAAGCC 
ATCTTAACAG GTTATTTTTA TCATGTTGCC CGTTTAGGAG GTGGTGGAGG CTCATATCGT 
ACTTTAAAGC AACATCAACC CGTTTCAATT CATCCTAATT CATCATTGTT TGATGAAGAA 
CCTCGATGGG TTCTCTATCA TGAACTAGTT CTTACAACCA AAGAGTACAT GAGACAGGTT 
ATTGAAATTG AACCTAAATG GCTTGTAGAA ATTGCTCCAC ATTTTTATAA ATCCGAAGAG 
GTTGAAGATT TATGTAAGAA AATGCCGAAA AAAGTAGGAA AAGCAAAGGA AGAACTGGAA 
CGAAATTATT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC107359221 ( XM_015925693.2 ) cDNA ORF clone, Tetranychus urticae(two-spotted spider mite) -> XP_015781179.1 Tetranychus urticae pre-mRNA-splicing factor ATP-dependent RNA helicase DHX16-like (LOC107359221), mRNA.

Price & Availability↑

CloneID	OTe15710
Clone ID Related Accession (Same CDS sequence)	XM_015925693.1 , XM_015925693.2
Accession Version	XM_015925693.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2652bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-21
Organism	Tetranychus urticae(two-spotted spider mite)
Product	pre-mRNA-splicing factor ATP-dependent RNA helicase DHX16-like
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015449932.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 22, 2018 this sequence version replaced XM_015925693.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Tetranychus urticae Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCATCGA GTAAACAAAC TTCATCAAAT CATGATGAAG AAGAAAGACT CAAAGATATT 
GCAGAAAGAG ATGCTTATGC AGAGAGACTC AGAGAAAAGG ACCAAAAAAG GACTCGTAAT 
GTGGCCACCA AATCAGATGA AAGGATGTAC GCTGAAGCAG CGAAAAGACT AAAACTGGAA 
AATGAGGATA GAAAAGCTAT AGTACCAAAA CTGCGAGAGG AATCGAGAAA AAACTATCTA 
ATCAAACGAA AGGCCGATCA ATTGGTTTTG TTAGAAGGGG AAATTAAGGA TGAAGAACAG 
ATTTTCAGTG ATGTACCCTT AACTAAAGCT GAAAAGGCTG AATATGAATA CAAGAAAAAG 
GTTCTTACTT TGGCTAAGGA ACATGAACAG GCTAAAGAAT TGGAAAGTAT TAGAAGATAT 
CACATTCCAG AAGAAAGTAA ACCAAGCAAA GTTTATGAAG AAATCGATGA AAGGGAGAAA 
CAACCAAACG CTGAAGCTAG AAAATGGGAA GAAGAAAGGT TAAATACTGC CAAATTATCA 
TTCGGAGCAA AAAATAAATC CAAAAAGGTT GAAGAATATG ATATTTTAAT TGATGCTTCA 
ATCGACTTCA TCAAAGCACT AACGGTTGGT GGACAAAATA CTGACGAAAA AGATATAGTT 
GACGAAGAAA CAGCAAAAAG AATGACAATT GCTGAAACAA GAAGATCATT ACCGATTTAT 
GCTTACAAAA GAGAACTCAT CAAAGCAGTA AAAGAGCATC AAATTTTAAT CATTGAAGGA 
GAGACAGGAT CTGGAAAGAC AACACAAATT CCCCAATATT TACATGAAGC AGGTTTCACC 
AAAGGAGGTA AAAAAGTTGG TTGCACACAG CCAAGAAGAG TAGCTGCTAT GAGCGTAGCG 
GCCCGTGTTG CTCAAGAAAT GAATGTTAAA CTTGGCCATG AAGTCGGTTA TAGTATTCGT 
TTTGAAGATT GTACTAGTGA GCGAACTGTG ATTAAATACA TGACTGATGG TATGTTACTC 
CGAGAATTCC TCTCTGAACC TGATTTAGCA TCTTATTCAT GCTTGATTGT TGACGAAGCA 
CACGAGAGAA CCCTTCATAC AGACATTTTG TTTGGCTTAG TTAAAGATAT AGCTCGATTT 
AGACCTGACT TTAAGCTTCT CATCTCCAGT GCCACTCTGG ATGCCGAAAA ATTCAGCGTC 
TTTTTTGATG AAGCTCCAGT TTTTCGTATA CCAGGTCGTC GGTTTCCAGT TGATATTTAT 
TACACCTCAG CACCTGAAGC TGATTACATA GATGCCTGTG TAGTTACTAT TTTACAAATA 
CACATCACAC AACCTTTAGG TGATATTCTC GTCTTTCTTC CCGGTCAAGA GGAAATCGAA 
CAATGTTCTG AAGTTTTGAT GGAACGACAA CGAAAGTTAG GATCAAAAAT CAGGGAACTT 
ATAATCAGAC CAATTTACGC TAACTTGCCT CAAGAATTAC AAGCTCAAGT TTTCGAACCA 
ACTCCACCGG GTGCTCGGAA AGTTGTAATT GCTACCAACA TTGCTGAAAC TTCATTAACA 
ATAGATGGTA TTATTTATGT CATCGATCCT GGTTATTGTA AATTAAATAT TTATAATCCG 
AGATCTCGTA TGGAATCTTT AGTTGTCCAA CCAGTCTCTA AAGCTAGTGC TAATCAAAGA 
GCCGGTAGAG CTGGTCGTGT TGCTGCCGGT AAATGTTTTA GACTGTTCAC TGCATGGGCA 
TATCAGAATG AGCTTGAGGA TAATACTGTT CCTGAAATTC AAAGGGTTCA TCTTGGTAAC 
GCCGTTTTAC TTTTGAAAAG TTTAGGAATC AATGACATTA TTCATTTTGA CTTTTTGGAT 
CCACCGCCAT CTGAAACGCT TGTACTTGCC TTAGAACAAT TATATGCATT GGGAGCTCTT 
AATCAATTTG GTGAACTTAC TCGACTTGGT CGTAAAATGG CTGAATTTCC TATTGACCCA 
ATGATGTCCA AAGCTTTAAT TGCATCCGAA AAATATAACT GTAGTGTTGA GATAATGACC 
ATTTGCGCCA TGCTCAGTGT TAATTCCAAT ATTTTTTATC GGCCAAAAGA CAAAGCTATT 
CATGCAGATA CGGCAAGGAA AAACTTTTTT CAACCTGGAG GGGATCATCT GATGCTGTTG 
AATGTTTATA ATCAATGGGT TGAAATGGGA TATACTAAAC AATGGTGTGC TGAAAATTTT 
ATTCAATATC GAGCGCTAGT TAGAGCTCGA GATATACGAG ATCAACTAGA AAGTCTAATG 
GATCGAGCCG AAGTAGAAAT TAAATCAAAT CCAACTGAAT TTGTACTCAT CCGTAAAGCC 
ATCTTAACAG GTTATTTTTA TCATGTTGCC CGTTTAGGAG GTGGTGGAGG CTCATATCGT 
ACTTTAAAGC AACATCAACC CGTTTCAATT CATCCTAATT CATCATTGTT TGATGAAGAA 
CCTCGATGGG TTCTCTATCA TGAACTAGTT CTTACAACCA AAGAGTACAT GAGACAGGTT 
ATTGAAATTG AACCTAAATG GCTTGTAGAA ATTGCTCCAC ATTTTTATAA ATCCGAAGAG 
GTTGAAGATT TATGTAAGAA AATGCCGAAA AAAGTAGGAA AAGCAAAGGA AGAACTGGAA 
CGAAATTATT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015781179.1
CDS	202..2853
Translation	MSSSKQTSSNHDEEERLKDIAERDAYAERLREKDQKRTRNVATKSDERMYAEAAKRLKLENEDRKAIVPKLREESRKNYLIKRKADQLVLLEGEIKDEEQIFSDVPLTKAEKAEYEYKKKVLTLAKEHEQAKELESIRRYHIPEESKPSKVYEEIDEREKQPNAEARKWEEERLNTAKLSFGAKNKSKKVEEYDILIDASIDFIKALTVGGQNTDEKDIVDEETAKRMTIAETRRSLPIYAYKRELIKAVKEHQILIIEGETGSGKTTQIPQYLHEAGFTKGGKKVGCTQPRRVAAMSVAARVAQEMNVKLGHEVGYSIRFEDCTSERTVIKYMTDGMLLREFLSEPDLASYSCLIVDEAHERTLHTDILFGLVKDIARFRPDFKLLISSATLDAEKFSVFFDEAPVFRIPGRRFPVDIYYTSAPEADYIDACVVTILQIHITQPLGDILVFLPGQEEIEQCSEVLMERQRKLGSKIRELIIRPIYANLPQELQAQVFEPTPPGARKVVIATNIAETSLTIDGIIYVIDPGYCKLNIYNPRSRMESLVVQPVSKASANQRAGRAGRVAAGKCFRLFTAWAYQNELEDNTVPEIQRVHLGNAVLLLKSLGINDIIHFDFLDPPPSETLVLALEQLYALGALNQFGELTRLGRKMAEFPIDPMMSKALIASEKYNCSVEIMTICAMLSVNSNIFYRPKDKAIHADTARKNFFQPGGDHLMLLNVYNQWVEMGYTKQWCAENFIQYRALVRARDIRDQLESLMDRAEVEIKSNPTEFVLIRKAILTGYFYHVARLGGGGGSYRTLKQHQPVSIHPNSSLFDEEPRWVLYHELVLTTKEYMRQVIEIEPKWLVEIAPHFYKSEEVEDLCKKMPKKVGKAKEELERNY

Target ORF information:

RefSeq Version	XM_015925693.2
Organism	Tetranychus urticae(two-spotted spider mite)
Definition	Tetranychus urticae pre-mRNA-splicing factor ATP-dependent RNA helicase DHX16-like (LOC107359221), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015925693.2

ORF Insert Sequence:

ATGTCATCGA GTAAACAAAC TTCATCAAAT CATGATGAAG AAGAAAGACT CAAAGATATT 
GCAGAAAGAG ATGCTTATGC AGAGAGACTC AGAGAAAAGG ACCAAAAAAG GACTCGTAAT 
GTGGCCACCA AATCAGATGA AAGGATGTAC GCTGAAGCAG CGAAAAGACT AAAACTGGAA 
AATGAGGATA GAAAAGCTAT AGTACCAAAA CTGCGAGAGG AATCGAGAAA AAACTATCTA 
ATCAAACGAA AGGCCGATCA ATTGGTTTTG TTAGAAGGGG AAATTAAGGA TGAAGAACAG 
ATTTTCAGTG ATGTACCCTT AACTAAAGCT GAAAAGGCTG AATATGAATA CAAGAAAAAG 
GTTCTTACTT TGGCTAAGGA ACATGAACAG GCTAAAGAAT TGGAAAGTAT TAGAAGATAT 
CACATTCCAG AAGAAAGTAA ACCAAGCAAA GTTTATGAAG AAATCGATGA AAGGGAGAAA 
CAACCAAACG CTGAAGCTAG AAAATGGGAA GAAGAAAGGT TAAATACTGC CAAATTATCA 
TTCGGAGCAA AAAATAAATC CAAAAAGGTT GAAGAATATG ATATTTTAAT TGATGCTTCA 
ATCGACTTCA TCAAAGCACT AACGGTTGGT GGACAAAATA CTGACGAAAA AGATATAGTT 
GACGAAGAAA CAGCAAAAAG AATGACAATT GCTGAAACAA GAAGATCATT ACCGATTTAT 
GCTTACAAAA GAGAACTCAT CAAAGCAGTA AAAGAGCATC AAATTTTAAT CATTGAAGGA 
GAGACAGGAT CTGGAAAGAC AACACAAATT CCCCAATATT TACATGAAGC AGGTTTCACC 
AAAGGAGGTA AAAAAGTTGG TTGCACACAG CCAAGAAGAG TAGCTGCTAT GAGCGTAGCG 
GCCCGTGTTG CTCAAGAAAT GAATGTTAAA CTTGGCCATG AAGTCGGTTA TAGTATTCGT 
TTTGAAGATT GTACTAGTGA GCGAACTGTG ATTAAATACA TGACTGATGG TATGTTACTC 
CGAGAATTCC TCTCTGAACC TGATTTAGCA TCTTATTCAT GCTTGATTGT TGACGAAGCA 
CACGAGAGAA CCCTTCATAC AGACATTTTG TTTGGCTTAG TTAAAGATAT AGCTCGATTT 
AGACCTGACT TTAAGCTTCT CATCTCCAGT GCCACTCTGG ATGCCGAAAA ATTCAGCGTC 
TTTTTTGATG AAGCTCCAGT TTTTCGTATA CCAGGTCGTC GGTTTCCAGT TGATATTTAT 
TACACCTCAG CACCTGAAGC TGATTACATA GATGCCTGTG TAGTTACTAT TTTACAAATA 
CACATCACAC AACCTTTAGG TGATATTCTC GTCTTTCTTC CCGGTCAAGA GGAAATCGAA 
CAATGTTCTG AAGTTTTGAT GGAACGACAA CGAAAGTTAG GATCAAAAAT CAGGGAACTT 
ATAATCAGAC CAATTTACGC TAACTTGCCT CAAGAATTAC AAGCTCAAGT TTTCGAACCA 
ACTCCACCGG GTGCTCGGAA AGTTGTAATT GCTACCAACA TTGCTGAAAC TTCATTAACA 
ATAGATGGTA TTATTTATGT CATCGATCCT GGTTATTGTA AATTAAATAT TTATAATCCG 
AGATCTCGTA TGGAATCTTT AGTTGTCCAA CCAGTCTCTA AAGCTAGTGC TAATCAAAGA 
GCCGGTAGAG CTGGTCGTGT TGCTGCCGGT AAATGTTTTA GACTGTTCAC TGCATGGGCA 
TATCAGAATG AGCTTGAGGA TAATACTGTT CCTGAAATTC AAAGGGTTCA TCTTGGTAAC 
GCCGTTTTAC TTTTGAAAAG TTTAGGAATC AATGACATTA TTCATTTTGA CTTTTTGGAT 
CCACCGCCAT CTGAAACGCT TGTACTTGCC TTAGAACAAT TATATGCATT GGGAGCTCTT 
AATCAATTTG GTGAACTTAC TCGACTTGGT CGTAAAATGG CTGAATTTCC TATTGACCCA 
ATGATGTCCA AAGCTTTAAT TGCATCCGAA AAATATAACT GTAGTGTTGA GATAATGACC 
ATTTGCGCCA TGCTCAGTGT TAATTCCAAT ATTTTTTATC GGCCAAAAGA CAAAGCTATT 
CATGCAGATA CGGCAAGGAA AAACTTTTTT CAACCTGGAG GGGATCATCT GATGCTGTTG 
AATGTTTATA ATCAATGGGT TGAAATGGGA TATACTAAAC AATGGTGTGC TGAAAATTTT 
ATTCAATATC GAGCGCTAGT TAGAGCTCGA GATATACGAG ATCAACTAGA AAGTCTAATG 
GATCGAGCCG AAGTAGAAAT TAAATCAAAT CCAACTGAAT TTGTACTCAT CCGTAAAGCC 
ATCTTAACAG GTTATTTTTA TCATGTTGCC CGTTTAGGAG GTGGTGGAGG CTCATATCGT 
ACTTTAAAGC AACATCAACC CGTTTCAATT CATCCTAATT CATCATTGTT TGATGAAGAA 
CCTCGATGGG TTCTCTATCA TGAACTAGTT CTTACAACCA AAGAGTACAT GAGACAGGTT 
ATTGAAATTG AACCTAAATG GCTTGTAGAA ATTGCTCCAC ATTTTTATAA ATCCGAAGAG 
GTTGAAGATT TATGTAAGAA AATGCCGAAA AAAGTAGGAA AAGCAAAGGA AGAACTGGAA 
CGAAATTATT GA